CN114807207A - Trichlorocarban metabolic plasmid pDCA-TCC and application thereof - Google Patents

Trichlorocarban metabolic plasmid pDCA-TCC and application thereof Download PDF

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CN114807207A
CN114807207A CN202210413124.7A CN202210413124A CN114807207A CN 114807207 A CN114807207 A CN 114807207A CN 202210413124 A CN202210413124 A CN 202210413124A CN 114807207 A CN114807207 A CN 114807207A
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pdca
triclocarban
tcc
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陈凯
乔文静
朱闻起
董浩然
张乐宁
罗子睿
柯壮
蒋建东
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Nanjing Agricultural University
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Abstract

The invention discloses a triclocarban metabolic plasmid pDCA-TCC and application thereof. The invention provides a plasmid pDCA-TCC for coding a triclocarban degradation pathway. The strain Pseudomonas putida KT2440 carrying the metabolism plasmid pDCA-TCC has a wider chloroaniline degradation substrate spectrum, can efficiently degrade antibacterial triclocarban, and can degrade toxic and harmful compounds such as herbicide anilazine, chlorpropham, plant regulator forchlorfenuron, insecticide diflubenzuron and the like. In urban domestic wastewater, 50 mu M trichlorocarban can be completely degraded by degrading bacteria KT2440(pDCA-TCC) within 3 days. The invention has good application potential and value in the biological strengthening restoration of the residual pollution of the triclocarban, and has important significance in the aspects of protecting the safety of the ecological environment, the health of human beings and the like.

Description

Trichlorocarban metabolic plasmid pDCA-TCC and application thereof
Technical Field
The invention belongs to the field of application environment microorganisms, and relates to a triclocarban metabolic plasmid pDCA-TCC and application thereof.
Technical Field
Triclocarban (3,4,4' -trichlorocarbanilide) is a synthetic broad-spectrum halogenated aromatic antibacterial agent that primarily inhibits gram-positive bacteria and has partial antibacterial activity against gram-negative bacteria and fungi. Over the past 60 years, triclocarban has been widely used in pharmaceutical and personal care products such as cosmetics, body washes, detergents, deodorants, and the like. A large amount of triclocarban is consumed worldwide every year and discharged to sewage treatment plants. Although the U.S. Food and Drug Administration (FDA) prohibited the use of triclocarban in daily necessities such as hand sanitizers and toilet soaps in 2013, triclocarban still remains a great market in china and india. Due to the poor water solubility and the intractable nature, 84-11,400 ng L of water is detected in the influent water of sewage treatment plants in the United states, Canada and China respectively -1 、4～270ng L -1 And 4.7-170.0 ng L -1 The triclocarban of (1). Due to insufficient treatment, about 3% of triclocarban remains in the effluent of the sewage treatment plant and 75% of triclocarban is adsorbed in the dewatered sludge. In China, triclocarban is often presentDetected in various aquatic environments, ranging from a few nanograms to a few nanograms per liter. Research has shown that triclocarban residues in water are toxic to some aquatic organisms, such as algae, crustaceans, and fish. In addition, triclocarban can accumulate through the food chain in mammalian and human tissues, leading to endocrine dysregulation, potential carcinogenic and genotoxic effects. Triclocarban has been listed as one of the ten emerging environmental pollutants in the world. Therefore, there is an urgent need to take measures to eliminate the potential environmental problems and health risks of triclocarban and its toxic intermediates. Based on chloroaniline metabolic plasmids which are wide in host range and can be autonomously transferred, a metabolic plasmid capable of completely mineralizing triclocarban is constructed by inserting key enzyme genes of the first step of triclocarban degradation, and the plasmid is introduced into a safe and harmless model microorganism to obtain a recombinant strain capable of degrading triclocarban. The recombinant strain is inoculated into the water body polluted by the triclocarban, and the residual triclocarban in the wastewater can be effectively removed through the genetic biological enhancement mediated by plasmids. The genetic biological enhancement mediated by the triclocarban metabolic plasmid can be widely applied to the biological repair of triclocarban pollution in the environment, and has wide application prospect.
Disclosure of Invention
The invention aims to provide a trichlorocarban metabolic plasmid pDCA-TCC.
Another object of the present invention is to provide a recombinant strain Pseudomonas putida KT2440(pDCA-TCC) that degrades triclocarban.
It is still another object of the present invention to provide the use of the triclocarban metabolic plasmid pDCA-TCC.
The purpose of the invention can be realized by the following technical scheme:
the nucleotide sequence of the triclocarban metabolic plasmid pDCA-TCC is SEQ ID NO.1, the total length is 88534bp, and the pDCA-TCC contains 93 predicted ORFs which are responsible for plasmid replication, maintenance, conjugal transfer, recombination and catabolism. The gene for coding the trichlorocarban degrading enzyme mainly comprises a trichlorocarban hydrolase gene tccA2, a chloroaniline dioxygenase gene cluster dca and a chlorocatechol dioxygenase gene ccdC.
The total length (from the start codon to the stop codon) of the trichlorocarban hydrolase gene tccA2 contained in the pDCA-TCC is 1425bp, the gene is located from 75155bp to 76579bp of SEQ ID NO.1, the content of G + C is 68%, 474 amino acids are coded, and the molecular weight is 50 kDa.
The amino acid sequence of the hydrolase TccA2 coded by the trichlorocarban hydrolase gene tccA2 is SEQ ID NO. 2.
The chloroaniline dioxygenase gene cluster dca contained in the pDCA-TCC is located at 5273bp to 8284bp of SEQ ID No.1, the total length of the gene cluster is 3012bp, and the gene cluster mainly comprises a dioxygenase gene dca 1A2 and a reductase gene dca B.
The chloroaniline dioxygenase gene dcaA1A2 contained in the pDCA-TCC, wherein the alpha subunit gene dcaA1 is located at 6938 bp-8284 bp of SEQ ID NO.1, the full length of the gene (from a start codon to a stop codon) is 1347bp, the content of G + C is 65%, 448 amino acids are coded, and the molecular weight is 50.6 kDa; the beta subunit gene dcaA2 is located in 6297bp to 6941bp of SEQ ID NO.1, the full length (from the start codon to the stop codon) of the gene is 645bp, the content of G + C is 67%, 214 amino acids are coded, and the molecular weight is 23.9 kDa.
The chloroaniline reductase gene dcaB contained in the pDCA-TCC is located at 5273bp to 6280bp of SEQ ID NO.1, the full length (from a start codon to a stop codon) of the gene is 1008bp, the content of G + C is 69%, 335 amino acids are coded, and the molecular weight is 35.6 kDa.
The chlorocatechol dioxygenase gene ccdC contained in pDCA-TCC has the total length (from the start codon to the stop codon) of 726bp from 82531bp to 83256bp of SEQ ID No.1, the G + C content of 60%, 241 amino acids in the code and the molecular weight of 27.2 kDa.
The invention relates to a triclocarban degrading bacterium containing the triclocarban metabolic plasmid pDCA-TCC.
Preferably, the host bacterium is Pseudomonas putida KT 2440.
Advantageous effects
The invention provides a metabolic plasmid pDCA-TCC for coding trichlorocarban with high efficiency and rapid degradation. Anb-1, a primary host containing multiple antibiotic resistance, is a wild-type plasmid pDCA, which is not only difficult to genetically engineer, but also has a significant ecological risk. The well-known safe and harmless model strain Pseudomonas putida KT2440 in a laboratory is selected as a new host of the plasmid pDCA, so that the genetic modification of the plasmid is easy, and the new host bacteria are safe and friendly to the environment. The wild type plasmid pDCA encodes a whole set of enzyme system for chloroaniline degradation, but cannot act on triclocarban; after introducing the trichlorocarban hydrolase gene tccA2 on the plasmid, the trichlorocarban can be converted into chloroaniline, which is then continuously degraded completely by the chloroaniline-degrading enzyme system. The trichlorocarban hydrolase gene tccA2 is inserted into a non-coding region (75123-75364) of an accessory region of a wild plasmid pDCA, the structure of a plasmid skeleton gene is not damaged, and the normal physiological functions of plasmid replication, conjugation, transfer and the like are not influenced. The strain Pseudomonas putida KT2440 carrying the metabolism plasmid pDCA-TCC has a wide degradation substrate spectrum, can efficiently degrade triclocarban, and can also degrade a series of chloroaniline derivatives, including toxic and harmful compounds such as herbicide anilazine, chlorpropham, plant regulator forchlorfenuron, insecticide diflubenzuron and the like. The recombinant bacterium KT2440(pDCA-TCC) can be used for pollution remediation of triclocarban residues in urban domestic wastewater, and can completely degrade 50 mu M triclocarban within 3 days. The metabolic plasmid pDCA-TCC provided by the invention can also be used for biologically and intensively repairing the pollution of benazolin, chlorpropham, forchlorfenuron, diflubenzuron and the like in the environment. The microbial inoculum carrying the metabolic plasmid is used for biologically strengthening polluted environment, has the advantages of low production and use cost, convenient use, good restoration effect and the like, has good application potential and value, and has important significance for protecting ecological environment and maintaining human health.
Drawings
FIG. 1 Gene map of plasmid pDCA-TCC
FIG. 2 Strain KT2440(pDCA-TCC) generates hydrolysis circles on LB plates containing 0.6mM triclocarban
FIG. 3 degradation curves of Strain KT2440(pDCA-TCC) against Trichlorocarban
FIG. 4 degradation substrate spectra of Strain KT2440(pDCA-TCC)
FIG. 5 biological enhanced repair of trichlorocarban polluted water body by strain KT2440(pDCA-TCC)
Detailed Description
Example 1 construction of Trichlorocarban metabolism plasmid
1.1 plasmid pDCA extraction
Achromobacter sp.ANB-1 (Zulong, separation of herbicide linuron and degradation strain of metabolite thereof, degradation enzyme gene and flora cooperative metabolism research [ D ]. Nanjing: Nanjing agricultural university, 2017) as an original host of plasmid pDCA is cultured in an LB culture medium in large quantity, DNA of the plasmid pDCA with higher purity is extracted by an alkaline lysis method and dissolved in Tris-EDTA (pH 8.0) buffer solution, and the specific method refers to the 'finely compiled molecular biology experimental guidance' compiled by F.Osber and the like. The quality and concentration of the DNA samples were determined by agarose gel electrophoresis and NanoDrop 2000(Thermo Fisher Scientific, Shanghai, China), respectively.
1.2 construction of recombinant Strain Pseudomonas putida KT2440(pDCA)
Anb-1 contains multiple antibiotic resistance as the original host of plasmid pDCA, and it is difficult to genetically engineer the plasmid pDCA that it carries. Therefore, pDCA must be transferred to a new host that is easy to genetically engineer. ANB-1 as donor, selecting model strain Pseudomonas putida KT2440 which is common in laboratories and safe and harmless as recipient, transferring plasmid pDCA into strain Pseudomonas putida KT2440 by two-parent conjugation, and obtaining recombinant strain Pseudomonas putida KT2440 (pDCA).
1.3 cloning of Trichlorocabam amid hydrolase Gene tccA2
After the strain LR2014-1 (deposited in China center for type culture Collection, the preservation number of the strain is CCTCC M2015236) is cultured in a large quantity, genome total DNA with high purity and large fragment is extracted by adopting a CTAB method, is dissolved in Tris-EDTA (pH 8.0), and is preserved at the temperature of-20 ℃ for subsequent experiments, and the specific method refers to the 'finely compiled molecular biology experimental guidance' compiled by F.Osber and the like.
1.4 PCR amplification of amidase Gene tccA2 and upstream and downstream homology arms
The following primers were used:
Kn-1：GATAAGCTTGTGATCGGCGGCGTCTAT(Hind III)
Kn-2：CCCGTTCCTCTCGGTTTATGAACAGGCACTGACTAACGTG
Kn-3：CACGTTAGTCAGTGCCTGTTCATAAACCGAGAGGAACGGG
Kn-4：TATTTTCAACCACGACAGGAGGGGTTGGTCGAGGCTCACAG
Kn-5：CTGTGAGCCTCGACCAACCCCTCCTGTCGTGGTTGAAAATA
Kn-6：CAATCTAGAGTCATAGATAGCCGCGTTCA(Xba I)
wherein, the extracted DNA of the plasmid pDCA is used as a template, and primers Kn-1/Kn-2 and Kn-5/Kn-6 are used for respectively amplifying an upstream homology arm and a downstream homology arm. The trichlorocarban hydrolase gene tccA2 was amplified using the extracted genomic DNA of strain LR2014-1 as a template and primers Kn-3 and Kn-4.
An amplification system:
Figure BDA0003603435810000041
PCR amplification procedure:
denaturation at a.98 ℃ for 2min
b.98 ℃ denaturation for 15s, 60 ℃ annealing for 15s, 68 ℃ extension for 120s, 30 cycles
c, extending for 10min at the temperature of 68 ℃,
d.10 ℃ for 5min, and cooling to room temperature.
1.5 construction of Trichlorocarban metabolism plasmid pDCA-TCC
The primers Kn-1 and Kn-6 are used, the upstream homology arm, the downstream homology arm and the tccA2 gene fragment amplified in 1.4 are used as templates, the three fragments are connected by overlap extension PCR, and are cloned to a suicide plasmid pK18mobsacB after enzyme digestion and enzyme connection, so that the recombinant suicide plasmid pK18-tccA2 is obtained. The suicide plasmid pK18-tccA2 was transferred by electrotransformation into the strain Pseudomonas putida KT2440(pDCA) for homologous recombination. After double crossover, positive clones were finally picked on 10% sucrose-containing plates and colony PCR verified for positive clone KT2440(pDCA-TCC) (FIG. 1). The picked positive clones were spotted on LB plates containing 0.6mM triclocarban. The production of hydrolysis circles was observed around the positive clones after 3 days of culture at 30 ℃, but no hydrolysis circles were produced around strain KT2440 which did not carry the metabolic plasmid pDCA-TCC (fig. 2), indicating that the positive clones carrying pDCA-TCC can hydrolyze triclocarban.
2. Function of trichlorocarban metabolic plasmid pDCA-TCC
2.1 seed liquid preparation
The strain KT2440(pDCA-TCC) is inoculated into 100mL LB culture medium containing 0.1mM trichlorocarban, shaking culture is carried out at 30 ℃ and 150rpm, the thalli is collected by 6000rpm centrifugation after 24 hours, the thalli is washed twice by using inorganic salt culture medium (MSM), and finally, 10mL MSM is used for re-suspension to be used as seed liquid for standby.
2.2 degradation of Trichlorocarban by Strain KT2440(pDCA-TCC)
The triclocarban in the aqueous phase was extracted with an equal volume of dichloromethane. In N 2 The organic phase was blown dry down and the residue was dissolved in methanol for HPLC analysis. Detection conditions are as follows: the HPLC is a Dionex Ultimate 3000SD HPLC system equipped with a C18 reverse phase column (4.6X 250mm, 5-. mu.m particle size; Dionex). The mobile phase consists of 60% methanol and 40% water, and the flow rate is 1.0mL min at 30 deg.C -1 The amount of the sample was 20. mu.L. The detection wavelength of the triclocarban is 275nm, and the detection wavelength of the 4-chloroaniline and the 3, 4-dichloroaniline is 250 nm.
Strains KT2440(pDCA-TCC) and KT2440(pDCA) were inoculated at 5% inoculum size to 100mL MSM containing 50. mu.M triclocarban, shake-cultured at 30 ℃ and 150rpm, and 5mL was sampled every 6 hours and taken up to 48 hours. And detecting the residual amount of the triclocarban in the sample, calculating the degradation rate, and drawing a time-degradation curve of the strain to the triclocarban. As shown in FIG. 3, the strain KT2440(pDCA-TCC) was able to completely degrade 50. mu.M of triclocarban in 36 hours, and during the degradation, the produced intermediate metabolites, 4-chloroaniline and 3, 4-dichloroaniline, were completely degraded in 48 hours, whereas the control group KT2440(pDCA) was unable to degrade triclocarban.
2.3 Effect of temperature on degradation of Trichlorocarban by Strain KT2440(pDCA-TCC)
Inoculating seed liquid of a strain KT2440(pDCA-TCC) into an MSM culture medium added with trichlorocarban with the final concentration of 50 mu M according to the inoculation amount of 5 percent, carrying out shake culture at 150rpm under the conditions of 4, 16, 30, 37 and 42 ℃, sampling after 48 hours, detecting the residual quantity of the trichlorocarban, calculating the degradation rate, and determining the influence of the temperature on the degradation of the trichlorocarban by the strain KT2440 (pDCA-TCC). The result shows that the KT2440(pDCA-TCC) has the highest relative degradation rate to the triclocarban at 30 ℃, can well degrade the triclocarban at the temperature of 30-37 ℃, and has the relative degradation rate of over 90 percent; when the temperature is lower than 16 ℃, the relative degradation rate of the strain KT2440(pDCA-TCC) to the triclocarban is reduced to be below 60 percent.
2.4 Effect of inoculum size on degradation of Trichlorocarban by Strain KT2440(pDCA-TCC)
Inoculating the mixture into MSM culture medium containing 50 μ M triclocarban according to the inoculum sizes of 2%, 5%, 10%, 15% and 20%, performing shake culture at 30 deg.C and 150rpm, and measuring the content of triclocarban after culturing for 24 hr. The determination result shows that the triclocarban is completely degraded in the culture medium with the inoculum sizes of 10%, 15% and 20%; in the culture medium with the inoculum size of 2 percent and 5 percent, the degradation rate of the triclocarban is 84 percent and 93 percent respectively. Therefore, the inoculation amount has a direct relation with the degradation efficiency of the triclocarban, and the larger the inoculation amount is, the higher the degradation efficiency of the triclocarban is.
2.5 degradation substrate Profile of Strain KT2440(pDCA-TCC)
Inoculating strain KT2440(pDCA-TCC) into MSM culture solution containing anilazine, chlorpropham, forchlorfenuron and diflubenzuron at 5% of initial inoculation amount, and performing shake culture at 30 deg.C and 150 rpm; after culturing for 48 hours, adding dichloromethane with the same volume into the culture solution, shaking and extracting for 1 hour by a shaking table at 200rpm, standing for 30 minutes, absorbing 3mL of organic phase when the aqueous phase and the organic phase are separated, and adding a small amount of anhydrous sodium sulfate to dehydrate the organic phase; accurately sucking 1mL of organic phase in a quartz cuvette, and detecting the metabolite by using an ultraviolet spectrophotometer. As shown in FIG. 4, the strain KT2440(pDCA-TCC) had hydrolytic activity against all of anilazine, chlorpropham, forchlorfenuron and diflubenzuron.
3. Application of pDCA-TCC (polyvinyl pyrrolidone-beta-cyclodextrin) in biologically enhanced repair of triclocarban in sewage
Strain KT2440 carrying trichlorocarban-metabolizing plasmid pDCA-TCC (pDCA-TCC) was used) The wastewater collected from the Tokyo sewage treatment plant (WWTP) is subjected to bioaugmentation treatment. Strain KT2440(pDCA) carrying the wild-type plasmid pDCA was used as a control. The collected wastewater naturally settles at room temperature. After removal of the supernatant, the sludge was inoculated at 10% (w/w) into a 250mL Erlenmeyer flask containing 90mL wastewater, and 50. mu.M triclocarban was added. The cultured cells of strains KT2440(pDCA-TCC) and KT2440(pDCA) were harvested, washed twice and inoculated into wastewater to a final cell density of about 2X 10 7 mL -1 . Treatments not inoculated with both strains served as abiotic controls. All treatments were performed in triplicate. Each treated culture was incubated on a shaker at 25 ℃. To simply simulate the aeration and sedimentation processes in the wastewater treatment process, the vibrator was set to vibrate at 160rpm for 12 hours, and left to stand for 12 hours, followed by 5 days of circulation. Taking 2mL of mixed solution sample every 24 hours, centrifuging at 12,000rpm for 5 minutes to collect supernatant, adding 2mL of dichloromethane, violently shaking for 5 minutes, standing for 10 minutes, separating an aqueous phase from an organic phase, absorbing the organic phase, adding a small amount of anhydrous sodium sulfate to dehydrate the organic phase, accurately absorbing 800 mu L of the organic phase into a 1.5mL centrifuge tube, drying by using a nitrogen blowing instrument, adding 400 mu L of methanol, carrying out ultrasonic treatment in an ultrasonic cleaner for 10 minutes, filtering impurities by using a 0.22 mu m organic phase filter, and detecting by HPLC. As shown in FIG. 5, in the inoculated strain KT2440(pDCA-TCC) bioaugmentation group, the addition of 50. mu.M triclocarban was completely degraded within 3 days, and the accumulation of the intermediate metabolites 3, 4-dichloroaniline and 4-chloroaniline was detected by HPLC. After 5 days, the accumulated 3,4-DCA and 4-CA degraded to undetectable levels. While the triclocarban concentration in the inoculated strain KT2440(pDCA) and the uninoculated control group also decreased slightly, but no accumulation of intermediates was observed, indicating that triclocarban was not degraded, and a small reduction in triclocarban could be adsorbed by the sediment in the wastewater. The research result shows that the inoculation of the host strain Pseudomonas putida KT2440 carrying the trichlorocarban metabolic plasmid pDCA-TCC can effectively remove the trichlorocarban residue in the wastewater.
Sequence listing
<110> Nanjing university of agriculture
<120> trichlorocarban metabolic plasmid pDCA-TCC and application thereof
<160> 2
<170> SIPOSequenceListing 1.0
<210> 1
<211> 88534
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 1
ggtacggttg ctttccggca gccccgaagg gtgcaaggag aagcaatgaa gaggtcgaga 60
ttttcggaag agcagatcgc ctacgcgctg cgactggccg agtcgggcac acccgtcatc 120
gatgtgtgtc gccagatcgg gcgtgtccga agcgacgtac tacacgtgga agaagaagtc 180
ggcgacctgg gcgtcaccga gctcaagcgg ctcaagatgc tcgaggacga gaacgcccgc 240
ttgaagcgca tcgtggctga cctgacgctg gacaagcaga tcctgcagga ggtcgttaga 300
aaaaagctct gaaggctgcc aagcggcgcg agctggcagc ctggatgcag gagcgctacg 360
aggtgagtgt gcggcggtcg tgccgtctgg cgttgctgcg cccgtcggtt tggtatcaga 420
agagcgaggc acgcgatcaa agcgcgctgc gcctgcgcat tcgggagatt gcgatggatc 480
gtccacgttt cgggtatttg cgcgtgctgg tgatgctcaa gcgcgagggt tggtcggtcg 540
gcaagaagcg cgtctatcgc ctgtaccggc tggaaggctt gcagcttcgc atgaaggtca 600
aacgccgcaa gcgcatcagc ctgcagcgcg gtcgagcgac gccagccaca ggaccgaacc 660
agcactggag catggacttt gtacacgacc agatgctcga tggccgggca ttccgtgtgc 720
tcacggtgat cgatcagtgg agtcgcgaga gcgtgtctct agaggccgac ttccggctca 780
caggtcgatg cgtcgggaag gtcctgggac gaggctgcgc tgcagcgtgg atggcccaag 840
gcgatcacgg tggacaacgg cacggagttc acctccaagg cgctcgatga atgggcatgg 900
cgcagaggcg tgaaactcga ctacacgaga cccggaaagc ccacggacaa tgggctgatc 960
gagtcattca acggtcgctt gcgcgacgag ttcctgaacg tcaacgagtt cattacgatg 1020
caggatgcgc gtgagaagtt gaaggcttgg cagcatgact acaaccatca ccggccgcat 1080
ggctctctcg gccacctgac cccgagtgag ttcgtcaaaa agaggtcaga tcaacaagcc 1140
ggaagccgcc cgattccagt ttagaagtga ccggataagg gggagacgtc acttgcttgt 1200
attcaaacac tcagcaaggg atacgttttt cgggggcaag gtcactgaag ttgcgttgcg 1260
acatgcaagc gtgagacacg cggtgacgta ggctaaggaa cgcgggtgct cgaagagatg 1320
cgccccgtca ccgcttgcgc cgtcgctttc aacaattttg tcagcgctag cttcttgggc 1380
tcgaagatgc ttgtcggaac agctaggccg agacaggcga tcattttgag atggacattg 1440
aataccggtg cagagaaacc gacaacacca ttcgtaaact cgtcctgcga gtacgccacc 1500
ccctccgccc tcacactcag cacctgccga tgcagctctc ccaccgatct caccgaccgc 1560
cttgcaatcg gtcgcaactc gacgcgcgcc aagtattcct ccagctcctt cgcatcgaga 1620
aacgctaata cgagtttgcc ggaggagctt gcgtacagcg gcgcacgcac cccctcgtgc 1680
atcgcgagtc ctcgcgacgc cgtcacaccg tgcacacgct ccacaacatc ccccacgcga 1740
acattcaaac cgcacgtgtt aatttccatg caaccctgag ccggcatttc catcgaatct 1800
tgatccaccc tttttgcaag cctggaaggc tcacggtgtg gataagttct tggttttctc 1860
cttcttggat tgtgccggca gcgccgagct gttcttgaat cggaagctgt cgttgccagt 1920
ttccaggata tggcagtggt gcgtaagccg gtccaacagc gccgtcgtca tcttcgcatc 1980
accgaacacc gacgcccatt cgctgaagct caggttggtc gtgatcacga cgctggtgcg 2040
ttcgtaaagc ttggacagca ggtggaacag caaggcccca ccggacgcgc tgaatggcag 2100
ataacccagt tcgtcgagga tcaccagatc ggcgtacatg agccgggtgg cgatctgtcc 2160
cgacttgccc tgcgccttct cgacctccag cgcattgacc agttcgaccg tggagaagaa 2220
ccggactcgc cggtgatgat gctcgatggc ctgcacaccg agcgccgtgg ccaggtgggt 2280
cttaccagtg cccgggcccc cgaccaacac cacattgttg gcctcctcca gaaactcgca 2340
acggtgcaac tgccgcgcca gcgcctcatt gacttcgctg tgggcgaagt cgaaaccggc 2400
caagtcccgg tacacaggga aacgggccac cttcaattgg taggccacgg agcgcacctc 2460
gcgctcggcg gtttccgcct tgagcagttg ggccaggatg ggttgtgccg cctcgaacgc 2520
cggtgcccct tgtgcggtca gctcggccac ggcctgggcc atgccataca tcttgagctc 2580
tcgcagcatg atcacgatgg cgccgctggc agggtcatga cgcatgacgc acctcctggg 2640
ctgtgcgggc agccgcgggg cggcgcaact ggtcgtagcg cagcacgttg gcttgaggtt 2700
cgttgaccaa gcgcagggcc tgtggtgccg tcaccggcgc cgcaggcgcc ggctcccctt 2760
ccagcaaacg atgcagcacg ttcagcacat gggtcttggt cggcacgcca gcttccaagg 2820
ccagctccac ggcggccagc acggcctgtt catcgtggtg cagaaccagg gccaggatct 2880
ccaccatctc ccggtcgcca cccgattggc gcagcaacat ggcctgcagc cgtttgaagg 2940
ctggcggcag ttcggcgaac ggagcaccgt tgcgcaaggc accgggcttg cgctgcagca 3000
ccgccaggta gtgacgccag tcgtagacgg tccgaccacc gacgtcgtgg cgccggtcga 3060
tgaggcgcgt gtgctcgcac aagatctgac cttcggcggc gaccaccagc cgctcggcgt 3120
agacccgcag actcaccggc cggttggcgt aggaggccgg cacgctgtag cggttgcgct 3180
cgaagtgcac caggcaggtg ggcgagaccc gcttggtgtg ttcgacgaag ccgtcgaagg 3240
ggcgcggcat cggcatcagg gtgggccgct cctgctccca gacatcggcc accgtgcctg 3300
gcagcttgcc gtggacgatc tcgcgccaca aggtcttgca gcggttctcc agccactcgt 3360
tgagttcggc cagggttgga aacgccggca cgggttgcca cagccggtga cgcgcatcac 3420
gcacgttctt ctccacctgc cctttctccc agcccgaggc cggattgcag aactcggcct 3480
cgaacaggta gtggctgacc atggcgctga aacgggcgtt gacgtcgcgc tccttgcccc 3540
ggcgcacctt gtccacggcg gtgcgcatgt tgtcgtagat accgcgccgg ggaatgccac 3600
ccaacaccgc gaaggcatgg ttgtgcgcat cgaacagcat ctcgtgggtt tgcagcggat 3660
aagcccgcac ggtgaaggcg cgactgtggc tgagcttgaa gtgggctacc tgcagcttgg 3720
tgcgctcgcc gccgatgacg gcccagtctt cgctccagtc gaattggaac gcttcgccag 3780
gaccgaaggc cagcggcacg aaggtgcctc ggccggtggt ctgctgcgct tcctgacgtt 3840
gctgctgcca gactcgggcg aaggcggcta cccggccgta tgagcctggg tagcccaggg 3900
cggacaggtc ggcgtggagt tgcttcacgg tgcgccgctg cttgcgcgac ttgcccgctt 3960
cgctcttgag ccagcctgag agcttctcgg cgtagggatc gagctggctc ggactgatgc 4020
gccgagggta gtgcggctcc gccacacccg cgcgcaggta cttcttgatg gtgttgcgag 4080
acaggccggt gcgccgggcg atctctcgga tggacaactg ctcgcgcagg gcccagcgtc 4140
tgatgacact taatgttgcc acgtcaatca ctcctatgct cctgccgaaa aaatcagcag 4200
gttagggttg ttacgtggat cagttttaga cggaaattac cgggttaagc tgaggccaac 4260
gttggcgata gtgaccggca gcgccgccaa gcgcgggctg gtggtcagga cagggagcga 4320
ggtcattgag gccatgtcgc catttcttcc tgcatcctgt cggccaattc gcgctgccaa 4380
gctgccagca agtcaaagcg gctgaccagc cacagctccg tttcgaagaa gagcttttcg 4440
gggtacggca ccagttcggg atagtcagcg gcaacgaact tgggcagcag cgccacgccg 4500
tggcccagcg cgcacaggtg cgccatgggc acgaagctgt ccgactgcac gcgcaccttg 4560
cccgtcaggc cgaacttggc ccagtccagg ttggcgtagg tgaagttgtg atcctggtgg 4620
aagaactgca agttcatggc cagaaggtcg tggtcgtcga caaacgcggg atttgacgca 4680
ctcgtgtcgt ggcgcgccag gtacgcgcgg ctggcgtaag cgcccacggc caccgcaccg 4740
atcttgcgta cgcgcaggga attgctgttg cgcacaggcc gtgctagccg cacggcgatg 4800
tccacctggt tttgctccag attgacgaag tgcgtctcgg tggtgatgtt gagcagcagc 4860
cgaggaaatt tctcccccag cgcgacgaat actggcgcaa agcgcttggc gaacgcgtcc 4920
atcaggctga tgcgcaccac gcccgcgcct tcctggtgat tctgagagaa gctgcgcgcc 4980
gcctcttcca tgtgctgggc agcgctgacc agcgcgtgca ggtcggccga ggctttccag 5040
ccggcctcgc tgcgcacgaa cagagtgcga ccgaagcgct gctccagcac ccggatgcgc 5100
cggcgcaggg tggagtcgtc gatgccttcc atgtcggctg caacttcgta gctcccgtgg 5160
cgctccagcg cgaggaaggc gcggatcagg tcccatggag gtgtggtggt gccgtctcgg 5220
gtcatggtgc atgctttcat cgatggcttc aggctttctg gggcttgcag gttcaggaaa 5280
agcgcagccg cacctgggcg ctggcggcgc gcgtgcggca ggccagcagc cagccttctg 5340
cagcatcgtc ttcgtccagc accgagctgt cgagccgcag cacctcgccg tccaccaggc 5400
ggcacatgca ggaggcgcat tcgccaaccc ggcaagcgtg cgggacggcc agcccggcgt 5460
cgagcatggc gcccagcagg aattgcccgc cgcgcaccga cacggtatgc gtctgcccct 5520
tgagctgaac cgtcagctcg gcgtcgggac cctcggcgcc cgtttccacc gcgccctcca 5580
gcgcagcgcc gaagtcctcg cggtagacgc gactgggctc gatcccagcc gctgccagcg 5640
cagccagaac ggagtgcatg aaggcctcgg ggccgcaaag gtaggcgtca gcagcctcaa 5700
ggccctgggt ctgcgcctcc agcagcgcct gcgtgggcag gccctgttcg gcgtcatacc 5760
agtggcgtat ctccagccgc tgcccgctac ccgcctgtag cgcctgcaac tcagccagta 5820
gcatggccgt ggctcggtcg cggcaggcgt agaacagccg tacctggggc gcgccttgca 5880
gcaacgcttc gcgcgccagc gcgaagatgg gcgcaatgcc actgccgccg gcatacaaca 5940
gcactggttg gccatgccgc gccagtgtga agcgcccggc gggcggaagc acctgcaatc 6000
gttggcccac tcgcgcgtgg tcgcacagcc aattggagac gcgcccaccc gccacgcggc 6060
gcaccagcac gctgatcgcc tgcccatcct cgggcgcact ggtgatcgaa tagcagcgcc 6120
aatgttcatc gccttgcacg gggacggcaa gcgtcaggta ctggccagac tggaaggaaa 6180
aacgctgccg ctgcccgccg tccacgccca ggctcagcag gatggcgtcg ctgccttgcg 6240
gtgaaacagc ggtgacggta agagaatgtg ccagtttcat gcgctgccac ctcccctcac 6300
aaaaagaacg agttgttgcc ctgcaggcag tcgctgtcga tcaggttgac ctgcttgagc 6360
acgatgacta gcccctgggc gctctggcgc agcacgaacc cgttccagcc cgcgagcaca 6420
cggttgtggc cctcgcgtga ctcggccaga gtgaagctgt agcgcgcgcg ccattcattg 6480
ccacggtccg ggctgggcca cacttccagt ccgccccagt gccgcgccgt gcgcgaagtc 6540
gggaactgcg agaaggccag cccggtgccc aggcggctca cacggtcgag cagacggcgc 6600
cggtcgtgga attccaaggt gacgtattgg cgcgggtcgg gcgcgggcgc gcccgccggc 6660
acccaatagg cgcattcctc gccatacaac gccagccagc gctcgtaggc ggtcttgtcc 6720
atcgagccct gatcgagcag gcgagcttcc agtgtcagta gttgtgccac ctgctgcgtt 6780
tgcgcggcgt cagcgccctg ggtgcgcacg gcggcctggg ccaggtcgtc cgaagcggcg 6840
agaagccagt cgtagaacgc cggcaccacg tagagcgagt gaggctggtg gcggtaggcc 6900
ggaacggcgg cagcttgggc cagatcctgg gcggcgttca tggcagcttc cccttgttca 6960
tttgcagcgt cgggcgggct tgcatcagcc gcttccactg agcgtagtag ttgcgcatgt 7020
gaatctctgt ggtcaccggg ccgctgacca cgccatcggg accgggcaca tccttgccgg 7080
tctcccagtg gcgacccatg tccacccatt cgtcctcggg gctgttcata aggccgcgtt 7140
ggcactcttc gaagttggcg gcgtcgtcca tctcgcccat gacggggaaa tcctcctgcg 7200
tgcgcaggcg catggtgttg acctctgcgt ccacgtctct gcgccgggtg gcatgccagc 7260
ttaacgaggt ttcgcccacc gccagaggct ggatcacctg cacctggttg ccgatcagcg 7320
ccagattggg aaagatgttg aggttcatgc ccgccccgac agtggtgtcg agcacgcgtt 7380
gggcctgatc gcggctgtgg gtggcggcca cgtgggcctc gtagggctca cgccccggct 7440
gcgggcgctg ctcgcgccag gcggagttgc ggtgcatctc gggacgctgg tcgaccaccg 7500
tatgcccgtt gtcgagcgcg ttgttgctca tgcgggtgcg gtcggcgtcg gcgaagtagg 7560
ccatgtcgcc gccaccgtag cgttccttgg tcatcaccag cagcgactgg tgcgagaacg 7620
gcacgtggta gccgtcgccc gcgttgtccc agaccagttt ccagttgcag ccgacgcgaa 7680
agcgctgagc gccggccaag acgatgttct gagcgccccc attgtgctcg atccaggcgt 7740
ccagatagcg tgtggcattg ccgaggtgct cggccagggg cggcgcctcg gagttcaggg 7800
tagcgaagat gaagcccttg tacgacgcca cctgcgcagg ccgcgcaagg tgcatgtcgc 7860
gcgccttgaa ggccgggcca taggcgttct tgccgggaat gccgaacaac tgacctcggt 7920
tgtcgaaacg ccagccgtgg taggggcagg tgaagttggc ggcgtttcct tggtgctcgc 7980
ggcagacggt tgagccgcgg tgcgagcaac ggttggagaa cacgcgaatt tgcccctctt 8040
cgtcgcgagt cacgatgacc tcgcgggtgc ccagccaggc ctgccggaaa tcgttgggct 8100
cggggatctc gctttcatgg gccaagtaca cccagctggc ggcgtacaca tggtccattt 8160
cgcgggcaaa gatggcgggg tcggtataga cgcggcggtg tacacggtct ggctgcacca 8220
ggtcgtccca ttcatcggcg gcggattccc cggccggaac ggcggcaata tgaattactt 8280
tcatcgtttt ctcgcaattc ggttgaatct gaaaatcgcg gcttcagttc atgttgtttc 8340
ggtaagcgtg ggcacagccg cgggagccag ggcgccttcg tccagcttgc aggtcgtttt 8400
ctcgcaattc ggttgaatct gaaaatcgcg gcttcagttc atgttgtttc ggtaagcgtg 8460
ggcacagccg cgggagccag ggcgccttcg tccagtaagc ggcggcccag cgcgatgacg 8520
gcgtcgggag acagaggctg gctggcttcc tgttctacgt gcgcccactg ctcgtcgtcg 8580
aacagcgcgc ggtggtaggc catgaaattg tctttcagga actgacggtc cacctcgggg 8640
tgcccctgga tgcccaagat gcggccctgc accaagaaca cctcgtgggg gatggtctgc 8700
gacgacgcca gcaggacgct gcccggcggc agtgccgcca cgcattcgcc gtggctttgc 8760
accagcacgc tgggcgcttg cgccagcgcc gcgcccagca gctcaacggc gacggggtcc 8820
cagcgcaggg catcgacccc cagcttgaag cgccctgacg ggttcctggc aacccgtcca 8880
cctagggccg ccgcgatggc ctgcgagcca aagcacagcc cgatcagggg tgagcctgcc 8940
tcgtcgctaa cgcggcgaat cagcgccagc aggttgctca ccaggagcga atcggcatcg 9000
tccactaccg acattgggct gccgctgatg acgtagccgt cgtagcccat cgcccgctcc 9060
aggaagccgt cgcgcgcggg ttccagcacg tcccactgct cgcccgcgcg accgaaagtg 9120
gcgatcattt tttccttcaa gatctcgtct tcgggtactt cagtgcacca gagcacggca 9180
tatttcttct tcacggtgtc catgagcgtt acctggttca gttcagaaca gttcgaagta 9240
ctcgcgatgc tcccagtcgg tgacggggtc gcccgccatg tccacgtcgg cgccttcggc 9300
ggccacgaag cgcgcccatt cgctgcgccg caggtgcagc cagtagtgga tgaactcttc 9360
gccgaaggct tcgcggaaga agctggagcc ctccagcacg tccagcgcct cggccaggtt 9420
gcgcggcagg atcggaacgt cggcggcata gggcgtttcc tgcagagggc cgggatcgag 9480
ttggcgacga atgccatcga tccccgagaa gagctgcgag gccagataca ggtaggggtt 9540
ggccgccggc tcgccgaccc ggttttccac acggctggct gagtcttctg gggcggcgat 9600
cacgcgcgcc atggcggcgc ggttgtcctc ggcccaggtc acccgatccg gggccagaga 9660
gaacgggcgt cggcgtcggt agccgttaac cgtgggggtg gtgaagctgg aggccgcaca 9720
ggcatgtttg agcaggccac cgacataggc ccggcccagc gccgacagcg gctcgcccgt 9780
ctgcggaacg aaggcattga cgccggagtc gcgcgccgcc agcgactggt gcagatgcca 9840
gcccgaggca acgaaaccct caatcttggg tttgcacatg aaactggcaa ggtaaccgcg 9900
ccgccgacag atttgcttga ctgcgttgcg gaacaacacc atggtgtcgg ccatgtccag 9960
gccggacatg acgtcgaagg tggtctccat ctggctgggc ccaaattcat cctcgatgct 10020
gcgcaacggc atgcccagcg tcagcaggtg ctggcgcacc tcggccatga tgggttcgac 10080
ctcgtccaag tgattttcga gcagatagga gtagcccttg gcgaccggca tcaccttggg 10140
cggtgccgcc ggtgtgccgg ggccacccag cgtctcgggc tccaggcagg ggtcgacgat 10200
gcgcgtcaag taccactcca cctccaggcc accatggaag tcatagccca gatccgccaa 10260
ctgccccagc gctttcttca acagggcacg cgggctcagc gggaagggcc gaccgctacg 10320
catgtacaga tcggccagca tccagcccgt gcgatcagcc cagggcagca cgcggaaggt 10380
ggtggggtcg ggcaccatca ccacgttagg gctgcccgcc aactccgggg tgccaaaccc 10440
gccatccgag gaaaagggat tgaacacgaa ggtgctggcc gtgtcgaaga aaaagggcgc 10500
catggtgatt tccgagccgc tgccgaatgc ggagcgcaag gctgccaccg acagcatttt 10560
cccgcgcagc agaccatatt gatccggcca tgagaggcga atcatctgaa tgccgtcctg 10620
ctcgattttc ttcagcactt caacggccgc cgccttctgg gcgtcggacc acaagccgtg 10680
ccgatcaatg aatttctggc tcatttttgt ctccgggttt tccggcatag atgccggttg 10740
tttagaatta acgaattacc caaccatctg ccaccagatc agttcagcac gcattaaatt 10800
tgccaatcaa aattatgttg atttcctgag aacctgaaag gaaaaacatg cgaaacctat 10860
tcgcaaaaat gcgagtgatg caatggtttt ttcaaccacc ccccgccact tcgagcccta 10920
cggcaacgct aggagccacg cggcaggcta ggagcgtggg cggcagaagc agggaatccc 10980
ctgatgacct gcgcacatga gtgagcgttc aagctgcatg gccataagtt accgccccta 11040
cgaaccgcaa caagagatgc tgctgtccgc atcgctgcag gactggctgc ctgcggggca 11100
cctggcctac ttcatcagcg acacggtcga tgcgctgtaa gcgttccgcg aacccatctt 11160
tcatctcacc gttgaagctg cgaacctcgg gtcttcgcaa tcaacgaacg agacgaggtg 11220
gacatggatc gacagcagat ggaacggtgc ctggagcaag tggcggcgta tcgtgcctct 11280
ggccagaagg cgcaggtatg ggccgaggcc aacggggtgc cagcgggaac gctgcaaagc 11340
tggtgcgcac atgcgcggcg ctggcaggcg cggctcgacg gcgtcagccc tgagccctcg 11400
ccagccgcca ggccgagcgg cttcgtggcc gcccgcgtgg cgcctggtgc cgcatcgaca 11460
tcggtgcgcg tcgaattgaa cgtgggaggc acccggctcg atctgcattg gccgctggcg 11520
cacacgcgtg aactcgcttc gctgctgcgg gagttcggcc gatgattcgc atcgacgtca 11580
tctggctggc gcttggcgcg gccgacctgc gcgggggcat cgacacgctg ttggcccaag 11640
tggtgcgcgg cttcgcgctg ggcgcgcaag cccaccacgc ctacgtgttt gccaaccgcc 11700
gcgccgaccg gctcaaggtg ctggtctacg acggcgcggg catgtggctg tgcacccggc 11760
ggctgcaagc gggcagcttc gcctggccgc gccaggacac ggggtcactt caactcacgc 11820
gcgagcagtt cgactggctc gtcgcgggcc tgccgtggca gcgcctgggc agcgcgcagc 11880
cgcagtcgat cacggtggtc tgaatgcgct cgattcgagg tcgggatttg cgcgtttgtc 11940
tattggagaa agtcatgcct cgcgcgcacg cgcgcgcgag gcatgatggc ggccatgctc 12000
gaaggcccgg acacccacac gatcgatgcg ccgggcgcac tggattcgat gcaggagctg 12060
cgcgatgtga tcgggcgcat gcaaggcgag ctgaagttca agcaaaccag gatcgaggcg 12120
ctgaacttcg agatcgcgcg gctgaagcgc tggcgcttcg gctcctccag cgagagcctg 12180
gagacgagca cgcaggcggt gctgttcgat cagatcctgg ccgacacggc gctggaagac 12240
cgcgccgccc agcaagacca gaagccaccg gtagcgccac cccgggccaa aggccaggcg 12300
gtgcgccagg cgctgccggc gagcctgccg cgcatcgatc accatcacga gatcgagcag 12360
acccactgcg agtgcggcca gcccttcaag cgcatcggcc aagaagtcag cgagcagctc 12420
gattgcgtgc cggcccaatt cttcgtgctg cgccatatcc gcggcaagta cgcctgcacg 12480
tgctgccaga cgatccaggc cgcgccgatg cccgcgcaga tcatcgacaa aggcatcccc 12540
gcgccgggcc tgctcgcgca ggtggtggtg gccaagcacg acgatcactt gccgctgtac 12600
cggcaggaag agatctacgc gcgctctggc gtgcacatcc cgcgctcgag catggcccag 12660
tggatcggca tctgcggggt gcgtctggca ccgctggccg atgcactgaa gggcttcatc 12720
ctcagccacg gtgtgatcca cgccgacgag acgccggtgt cgctgctggc gccggggcgc 12780
ggcaagacca aacgggccta cgtctgggtc taccgcacga ccaacttcgt ggcccagcgc 12840
gcggtgctgt tcgacttcac cgccagccgc gccggcgaac atccgcggcg cgtgctgcga 12900
ggcttcggcg gcacgctggt cagcgacgat tacagcggct attttgggct tcaggcgcaa 12960
ggcgtcagcg ccgcactatg ctgggcccac gcgcggcgca agctgttcga ggcgcacgag 13020
ttcaacgcca gccagatcgc cggccaggcg gtggcgctga tcgcaaagct gtacgaggtc 13080
gagcgcgagg cgcgcgaact cgaaccccag gcacggtggc tgctgcgcca gcaacgctcc 13140
aggcccatcg tccaggccct gcacctctgg ctgaccgggc agcgccagaa gctggccaat 13200
gccgacgtga cggccaaggc gatcgattat tcgctgagca attggcgcgc gctcacgcgc 13260
tacctggacg acggcgacgt cccaatcgac aacaacgcgg cggagaacgc ggtgcgcccg 13320
ctgtgcgttg gccgcaagaa ttggcttttt gtaggctcgc agcaggccgg cgagcgcgct 13380
ggcgtggtca tgagcctgat cgagtcggcc aaactaaacg ggcacgatcc ctgggcctac 13440
ctcaaggacg tcttcgagcg tctgcccacg ctcaagcagc gcgacctcgc gcagctgctg 13500
ccgcacaact ggcggcctgc caccgacatc gccgtgccaa acgcggcgct cgccgccgcc 13560
gcgtagaacc atccttcaac acgccgtcat cgacggcgca agggtgtcgt tcgcggaacg 13620
cttacggtgt gccggcggcg ccggcgcagt tgctgccggt gcgaatcgag ccgaacctgg 13680
cgaccgtgcc gcagcgcgaa ggcggcctga ttgaagtgca gatccacggg gtcgatttgt 13740
tttcgttgtc aaaagtgacg gtaggagatc gagcactggc gtaggggtgg cctgccaagc 13800
cattgatttc attgggtttc ctgttgattt ttttgatcgc cgtttgaggg cgcaaaagcg 13860
tgctcggttt caaccgccgg tcccggtttt cggcgtggtc tgtgacggca ggatttcact 13920
ggcataacgc tccactggaa agcggaatac gcccctcaaa ttgatacctt gcagccgcgt 13980
cggcgcaatt ttccccgtga gctccgccgg gatgacctga cgccggttgg cccagcggtc 14040
cagaacggcc tgcatctggg ttgtgttcca ggccatgatg atgttggcca acagactcaa 14100
ggcgtcagcc accgcctgca tttcctcggc ccgcttggcc tgagccgggc tcactctgcc 14160
ggtataaatc gcccgcttca aggcattgac cgcctcaccc cggttgagta cccgacgcag 14220
ctcattcctg aaggcgtctt tgacaaagta gtcggccagg aacgcagttc gcagcaagcg 14280
ccccaactga acacccgctt cgtagatggg gtctccccgc gccgccgatc caaatctggc 14340
cagcgctgca actgcgctgg cgttgccgct catcaccgag gctgccaaat ggaccaaggt 14400
gtcccagtgc ttctcaatca aagcggtatc gacggtagcc tcgcaaactg ctgcgatctc 14460
cggtggaatg atcgtgccac gcggcacgta cagatgacgc tgcttcaatt ccttcagtcg 14520
tgggcataga tcgaacccca gtagccgggc caacgccatg gcaaagtcgg tgtagccgtg 14580
ggtgtccacc gccaactggc tggtctcgat ggattcttgg cgcacgacac cctcaatggc 14640
gacccctgcc tggcgctcat tgagcacaaa aggctgcgcg tagaagatcc cccatcggtc 14700
acgcacatgg gagtagatgc caatcgagga cgtgttgcgt cttggatcct gtcgggcctg 14760
ccacacccgt tgggtggttt ccatgctcat catgtcggac gacgccaggt ccgtgcgacc 14820
ccatgtcgct gcaatggggt gccgttgcat gaactccagc accgcgtggc aggcttgact 14880
caagcgacgc tcgtccccgg cccaacgcat ggcctgacgg atgctggtgg cagacaactg 14940
cggaatcata cgggcgcatt cggccgccgt cagactggtg ccgtgggcca tgatgccggc 15000
gtagaccatc agcagctctt ctgtcgaacg tggctcgcgg ccaagcatga tccagctgaa 15060
acgtacctga gcgtcaacgg ccaggatcac ttctgggagt tgcacctcac cgatcctcag 15120
gtcaagtttg gccctcaact taatcacctc tggatcttca tcctctgccg gcaatactga 15180
aaggtggagt tcttcgtcca cgcgcagcac accgctgcgc gcggcgacgg ccactgcctc 15240
cacgccggcg cgtaccttgg ccagcaacgg cttcaaaagt gtggatgcat tggctggcag 15300
tgacagtcgg gcatagtgcc gtttggcttc ttcctgccag cgcgcatcag tgaagaacag 15360
gcgtgcgcgg ccacgaaagc tcaggctgtg ctcaatccac accgaaccgt tgcgcacggc 15420
acgtcgcaat gaaaacaggg tggccacctc caaggcccga aacgcgcgct cccggtcggc 15480
acccgcaatc gcgccgctcc agaccgaacc caagcgaggg gcgctgacat cagcaggtag 15540
ttcgcgaagt ttgcccgcgt acagtttcgt caattgggcc aaggctgtgg tcaccgggtg 15600
ctcgctattg gcctgccagg gcagcttggt gatttcggcc agcaaagagc gcacgggacg 15660
tatggcgtca aacaaacgct cacgcaccac cgaggcccgg ctgggtggtt tgccttgctg 15720
gctggcactg agcagggcaa caattcgagc ccgcaactcc gcgtcttgaa gttcaccctg 15780
ggcgaccaaa ccagccagct ctgccagcaa tgtcttgtac aggtcagccc aattgaccgt 15840
ctccgtgaca cctgctgcga ccgtgcgcca aagatcggca acgcgccgct gaaccatcaa 15900
gatagcttgg tctgtgctgg taaacaggca gtgccgtaaa aagcagccaa cctccactgt 15960
gcgagcgggt tctttgatcc tggcccccac cgagggaggc cttgaagcca ggcggcgtgc 16020
atagcgccgc acaatgagac cggacaggtc gtccaggtgt ttgtggacat tcagggcgta 16080
cagcagttcg attcgctcga acacctcgcc aatttgtttg gttgagtgtt tggccggtgc 16140
cgaccacaac cagctctgct gggtttgccc atccgggcgc aatgcggcca tggcatcccg 16200
ccagcgcacc agttgttcgg gcagcacaga tgccgcaatc gttttggctg tttcttcttc 16260
gagttgtccc agcgccgctg tgaccaggac acggatgtca cggtcgcgca ggatcaggat 16320
cttgctttga tacaaccagc gccgagcgaa tacgagcaat tgatcccgat cggcgcagcg 16380
ggccacctca tcgcgcaggg tgcgtaccag ggcgcgacgt tggtgctccg tcatccaatg 16440
aaaccccagg acatcacagg ccagttgttg atggtcaaag agggttcgac cgcgaacata 16500
cagggctcgc agggatgcca gttcaacagc agtgatgccg atttcgtcac ccagatggcg 16560
ccacagggtt gtcggaacga tgcgcaccga attcagcagg cgcccgctca agcgcaagaa 16620
acctatgtgc agtgccagac ccagcttgtg ggcgttgctt cggcgggcgt tgatgagctc 16680
gcgctcagta cggctgtacg tgaaaaacga ctgaagctcg aaggtgctga tttcccttgg 16740
cagctctcgc aggccaaggt aggttgtgtg ccagctttgc atcttgtgtc tccggtgatt 16800
ggggcagaca caatacgcct gaaaaagtga acaggaaaac caatgaaatc aagggcttat 16860
cagaccaccc ctacgccagt gctcgatctc ctaccgtcac ttttgacaac gaaaacaaat 16920
cgacccctgt caaggaaaac gggcctgcag catcgccagg aacggcgatg gaggcccgct 16980
aggggacgcc agcagcgcag ttaaaacgtc gcgtctttca gcgagatcga acacccgttg 17040
tagtcgtagc tcacgtccga cacgacaccc gacacccgag cggttttccc ggttgaaagc 17100
tgggtgacgt tgagcttgtt gtctgtaccg gcatgcaccg aaacctggcc ggagcgcagg 17160
aacacccgat agcgaggctg gaagccttcg gtgacggagc gcacttcacc gctgaccgca 17220
aggcccttgt tggcatagag gccgtttgct cgcacctggt tgtctttcgc ggcctggcag 17280
atttgcggca gggattccgt gagcatcacc ggggcggtgg ccttctgcac atccttggcg 17340
acttgatcca acgcagcgca gcccgcgagg gcgagcaatg gaataacgag ggctagacac 17400
ttcttggttg attgcataga cacgaccttg agggttggtt aggaaattgg gtgtcgggat 17460
gcccccgact gctcaccact aggcaggtga cgcagccgct agaccgaagc gagcggcttt 17520
tagtacgtag gcacgtagat gacttcaatg tccacgcgct ggttctggtg ccgcccttct 17580
ggcgtcgagt tgtcggccac gaagtcggat gccgaagcga agttcaccat gatcttgagc 17640
ggcgacacgc cgcgcgcaat caggtactgg cgggccgaca aggcccggcg caaggccagg 17700
gcctcatcgg ccgcgctggg gcgtagcgtg ctggtgcgac cgctgacgta gatgatcgtg 17760
gcccccttcg cctcggccag cagctcggcc gtggaagcgt ccggccggaa cgtggtgctg 17820
ctgtcggaga aggacaccga aacagcggtc aagaccggct cgccatagct cacggccgcc 17880
atgccggcag cgccggcggc catcgcgcca ggcgcgaagg caagggacgc caaggagagg 17940
gtcagaaaaa gggctttgcg catggtgcgg actccaaatc agaagaacag gtagccggcc 18000
ttgctcatgg ggatgcggat caaccacaga gcggccaggt gcagcgggta ataggcgtag 18060
aacgcccagc gcaggcgcgg catgggcaga tcgacgcggg aagcgaccag gagcagcggc 18120
agcgaggcca gcgcccacag gttgcgattg atgaaccaca gcgccgcgca ggcgagcagc 18180
gcgacggccg cggcggccca ggtgggccgc ttgcggtacg accacaccgc caggccgaag 18240
gccacggccg gccaccagta ttcgaccagg ccgccaccga ccaggaacac caggccggcg 18300
ccgaccaggt gcagcgggcc gcccttttcc accaggtagg cgcacgccgt cacgaccagg 18360
agcgtgaaca agacattgag gggccaccac ccggcggaca ggccacccag ggcgacgaag 18420
ggcaccgagg ccacagcgcc gaacaccgcg aggcgcttca tcgtgcgcgt gtacgcgccg 18480
cgctcgaatg caccaggtcg agccaggttg taggccagca cgaaaacgaa gatcggcagc 18540
gccaggcggc cggcctcgaa gagaaacggc agcgtgccat tgaacaggta cttgttgacg 18600
tgatcgccgg tcatcagggc cagcgcgagc cacttcaacg cttcgaccgt cccatccggc 18660
acgtgcaagg gcttcatccg cgcttgcccc cgcgtgaggc ataggcgcgc agcaccagga 18720
agatgatgta cagcgcagga agcgacagca cgaaggcaat gggcttgccg atccatgccg 18780
gcagcgccca cagcagcaga cccagcgcga agatcgggcc gaggcccagc aagagcagat 18840
cgaattgccc cttggcctgc gattcggccc agttgcaata ggcggtcagc acccggccga 18900
aaccgagcgt gtagcgcatc atgaaggcgt ggaatttgtc catgttttag ccgctaaatc 18960
ggaaggcccg ccctactccg gcgagccttc gcttctgttg ttgaaggaga tcgagcgcgg 19020
cacgtagccg cccgcgacgt tgatggatgg agctgctgcc ggcctggccg ccggcgcggc 19080
cgctggggcc gctgccgtgg cctgctgggc ggcttgcacc gccggcgctg gcccggtcgg 19140
catcgagggc gtggcccccg ccttcgtcac gtccagcgtg acgaaccgag cttcgagcca 19200
atccgcccac ttggcggcct tgcgcatcag gtcggcgcga tacaccgcgt tgtactgcgg 19260
cgtgcgtgag tggtaattcg cggccttcgt ccagagatcg cccttgtcgt tgcgaatgtg 19320
catccgcaag cgccaggcgg cgaggtcgaa cgagtagcag ccagcggccg ctacatcgtc 19380
cgccgtgatg ccgtagcggg ccaggtcgcg caggtaagcc gtgttgaact gcatcggccc 19440
cacgtcatga gtgccgttcg agttgcgcac ccactggccc ggcttgccgc cttccttctc 19500
cgcgacggcc aacacgatgt tggccggcac ttcgtacttc acggccgccg aaacggcgca 19560
cacgacgcgc tcctggagct gcggcggaag gtcggcagca aagggcatgt cagtaacccc 19620
cccaggaagc gcggcggcgc tcttcgtcct cgcgccggat gcgttcgttg tactcggcca 19680
gcgcccggtc atagtcggcc gcttcgaccc aatacccgcc cctttctggc gtgccgacat 19740
agcgcggctt ggtgctgctg aaatcggtgt aagcgtggtt ggtgtaggcc gcgcagtagt 19800
cgggcatctg gttgctgatg tacgtcgtcc cgtcctcgta gccctgccag aacaccagcg 19860
tttgattcag ggcctgcgca tcgcaacggc cagcgccgcg cgacatggcc gacaccagcg 19920
cggacatttc cggcgtctgg ttcgacaccg ggcacagatc gaggaagttc ttgcgcttct 19980
tgatcgtgtc ggacattttc cgcgccgtga tgctgaaata cttgcgaagc gacggcgcgc 20040
attcgctcgg gcgggtgccg cttgcgaggc acaggatcgc ctcgcaagcc aggcgggtgt 20100
cgccggtcag cacgtcttgc gcgctggccg tgccgaccga gcagagggcc gccacagcgg 20160
ccacgatgga aagcaacttc ttcttcatga tgaacactcc tttgggttac gcagatttgt 20220
cgttgcggcc gccgtcacgg aacgcggcta cttcggactc gggatcggca gagctgtcag 20280
ctccggccag gctgttgtcg ccgaaggtcg gcgagggttg ttgatcgggc acgtcgatgc 20340
tcgtgtcggc cgagctgccg ccctgcgcct tgatcgcggc cgcgatcttt ccgccggtgg 20400
tgtcggcgat gcgttccatc gccgagtcgc gcatgctggc ggccttcgcg gccgccaccg 20460
cacctacacc cttcgccaag ttcgcgccgg cgtcggccgt gacgcggccg gccttgccaa 20520
gcgcggaagc ggccgaggcc agcatgccgg ggccggtact accaggtgcg tcctgctggc 20580
cgccctgccc cgctccctgg ccctggccgc caccctggcc ggccttcgcg ccgccggcgt 20640
caccgccgcc gctggccttc tggccgccgc cttcgccacc cttcgagctg gagccggcct 20700
tatcgccgcc ggccttgtcg ccgcctttgt cgccgccctt cgactcgccg ccgctgctgc 20760
cgccgccgcc agagtcgcca ccgccgccac cgaagccggc ggcttggccc aggggcgtgc 20820
tggtgccggc ttcaccgcca ccaccaccgc cagagtcgcc gccaccacca ccgcccagca 20880
tggacatgac atccgacccg gacgagacgt tttcattggc cttcgagaac gcggccatga 20940
ccgcagaagc accgccggcg gccgaggccg cgccggccgc cagagcagcg ccgccggttg 21000
ccgctgctgc ggccgccatg ccagcggcac cgagggccgc gcctgcgccg aagttgccga 21060
tgccggcacc gcccacgctc gcgccggtga tgacgccggc gatgagcgcc ggaatcttgt 21120
tgacgagtgc cagcaggatc acgcagacga tgagcatcac gcccatttcc ttgaggttga 21180
tcccctcgct catgcgcgcg tagtagtcat ccagaaaggt cttgccgatg ccgaccagca 21240
gcaccattgc catgagctgc gcggccacac ccagcacggt cttgtagtag ttgatcgcca 21300
tgtcggaagt ccaccgcgac ccaccgaagc cgaggaagaa cacgccgcca taggcgagca 21360
cccaaccaga cgccaggaga agaagcatgt tcacgccgat gagcgcgagc acgatcaaga 21420
tggcaacggc catgaggatg ccggccatgc tgtccaccgg cgaccacacc gaggactgat 21480
ccatcacctt gtagaagatg gcaaagccaa tatcgacaat gccggaaggc gacaaggctt 21540
gacccaagcc cgtcgcgttg ccggcaattt ggcgtagcga cgcatagatg gtcgaggcga 21600
agttagggcc gttcgtcagc aaccaccaga agaagccggt gaagatggtg aagcggacaa 21660
gctccgcgaa gaactcccca atgtcggcct tgcgcaaggc catcatgccg aacgtccaga 21720
ccatcgagat cgtcaccagc agccagaaca accaggatgc ggcgttggtg atgacaccgg 21780
cccagccgct ggcggcggcg ttgtagcggt tcagaacgtc atcgaagagg cccgagctgt 21840
tcatttgggc ggaagcgtcc gtagaaaaga acgccagcca aacgagcagc ggcagggcga 21900
gtttcaagct ggctggcatt ctcattcgtc agtacctcac caggacttag gttcgctagg 21960
tttgaacgag ccggcccgca ggcactgcga tgaaaacgcc tcttgcacgc tcttatcgag 22020
cttggcgatg ttctcgggtt tgcagttctc ggcattcact tcgggcagcg tcaccggctc 22080
gggctttttc tcgcagccgg ccagcagggc cgccacaagg gcggccgctg ccaggatcga 22140
gggtttggat aggcgcatgt atccccctta ccaattacgg gccggactgg ccctgtactc 22200
gcccttgcgc agcgcctcgc cggcggcttg ctgctgcgct tcacggtcgg cttcgacctg 22260
catccgcgtg gcgatggcgt tctgctgcgc gaccatgagg ccccggattt gcagaagctg 22320
gtttgcctgc tgggccgcga gctggttggc gaagccgatg gcctgcattt gcccgttcgc 22380
gccctgcgcg gccgactgga gccgttcgag ctggcgcgcg tcggacttca aggcgtcctg 22440
ctggcgatcc aggcccttga acaaggcatc gttggccttc ttctggctct cgctcgccag 22500
gcggcggttc tcggccattg cggcctgttc ggcgggcgtg cagccgccgg tgccgttgaa 22560
gcacggcgag ctgcggtagt aggccacgtc ctggaacttg cccaggtacg agtcgatgct 22620
gccgagctgg gtcttgtagt agttcagcgt atcgaccgca ttcatcagcc cgttgatcgt 22680
ggactgcgcc tgatcccaga tataggccgc cggcgcgacc gtgttctgga tcatgttttc 22740
gtactgctgg agctgggttt ggtactgctc aatttgcttg agcgtctgcg ccaccgactc 22800
gatggccgtc atgacgttct gcaccaggtt cccgccgtcg atgacgggaa tgccggcctg 22860
cgcgggcgac atggtggtga tcgacccggc catcagagtg acggcgagag cgactttagc 22920
ggctaaaaac ttctgcttca tttgctttct cctggcgtta gtagtcaaac gattggtacg 22980
gcttcgagaa cgtcatgtcc ttggtcacga tgacgttgaa ccgatagccg gggcggattt 23040
ccaaggtcgg ggcaatgttc agattcttgg aaacgagttg ggcgatggtg ttgcccagca 23100
cttgcccaag tgcttcgctc aaagcatcgc tggcgcgctg ctgattgccg gtggaaccgc 23160
tgttgtcctg gctcatgctc acggcggcga tgacgcccga catgaggaag gccgagccga 23220
acacgcgcag gtagtggtta ttcacctggt cattgaagcc cgcgtacccg gcgctatccg 23280
cgcccggcat cgtgccaatg tccatcgcct tgccatcggg gaacacgatg cgctgccaag 23340
ccacaagcac gcgcgactga ccgtaggcaa catcgctcga atacgacccg accaggcgcg 23400
agccttgcgg aatcagcaga tgcttgccgg tcggcgtgtc gtacacgttc tgcgcgacct 23460
gggccatgat ctggcccggc aagtcggagt tgatgcccga aatgagcgtg gccggcacca 23520
cgaagccggc gcgcagctcg aagggcgagc gcggcgcttc gggctgggag tccagccgcc 23580
aacggtcgcc ctggccgctt cccgcgaact gcgcgtagtt cttgccctgg ccgccaccgg 23640
cggtttgcaa gagctgcggc gcaccaccac ccacgccgcc gccggcctgc tggccgccga 23700
cgcccgaggc gcgaagctgc gcgagccggg cctgataggc ggcggtcgga tcgcccgatg 23760
cttgcgcacc gatttgctgc cgcacagccg ccaggcggga cagcatttcg tcgcgcgtct 23820
gcggcgtgcc cggctgcgcg cccgatgacc cgctgctgcg cggcgcatcg acacgaacgc 23880
ccgtcttggc cttgaccgct tcctggaaca tttgcagctt cgccatgcgg atgcggtcgg 23940
cttcatcgtc atgcaccggc tggcccaggt tgccgctggg cggtggcggc ggcgcgtcca 24000
ggtcggggcg cgcgacaagc accggcgcag cctctggccc ggtcgccagc tccggcggct 24060
tgagcggcga ggccgcttct accgtgccgc catccctgtc gccgacgatc tccttggcga 24120
acatcgacgt gttacccgcc ttctcggccg cgccttgcgc cggtgcgttc tgcttcgcag 24180
cgcgatccgc cgcgaccagc atcatcacca gcaggaaggc acccagcgcg gccgcaagga 24240
tgtacatcgg catgttgttg acacgccgca cgccggactt cttcgatacc gcgcccggcg 24300
aggcgtccgg tgccatttga tcggcttggt cactcatagg tcactccttg cgcacccaat 24360
acccagccgg cgcaaagctg ccgtcttgca ccaggtacgg gcgggtgatg gattgatggc 24420
cgaccaacag agtcaggcgg tacaggttcg agtcgccggc ctggtcgagc acgtaccgca 24480
gcggcagggc cgccggcgat gccggctcac tggccgccgt ggcctgcgcc ttcgcgctgg 24540
ccgggttgaa ctccagtagg gcatagccgc gctcgcgcaa cgtcagcacc agggcctggc 24600
cgaaggcatc aggcgtggcc tgttgcagct ccaggcgcgt cttggccggc gggtacagca 24660
cagcgagctg ctgcaccgct tcgcgggcga gctgctgctg atcgaccgtt gcggtcggcg 24720
ggacgaagtt gccgtattgg ccggtggtcg cgcagccgcc cagggcgagg acgaacagcg 24780
cagcaaagat gatcttgcgc atggtcactt cctccgggag atagtcacgc ggtcttggct 24840
tccaccaaca ccggcaatca ggatcgcgcg gtcaaacacc gtgtccacga tgtagcgatt 24900
gccctgcacg cgatagttca ccatcacggt ttcctcgtcg cggaagatgc cgccctcgcg 24960
gcgcacgacc aggagcgtcg gcgcttcggt ttgctccatc gttccgggca tttcgatgat 25020
ggtcttgcgg ccatcgttga acacacgcac cggcttccag ctcgccgagc cggacaggtc 25080
atagtcgaac ttgagatcgc ccaggtactc gccggtctgc gggatggtgc ggtcggtgcg 25140
ttcttgcgtt tcgcggcgct ggatcgcgtc ccacttcgca agcgcgtcct cggggtaggt 25200
gaacgagact tgcggcatgt actcggtgcg atgcgagcgc aggcgcaggt gataggcgcg 25260
gcggttcgtc gtcacgatga ggctggtttc caggccaaca tccatcggct tgatgatgag 25320
gtgctgcact tcgttggggc cgctgccggt gatggccggc tcgaccgtcc accgggccgt 25380
gtcgcccagg ttgatcgagt tgacttgctc gcctggctgc aaggccacgt cgcacacctg 25440
gagcaccgcg cacacgatgc tgggctgctg cgcgccgaac aggaacttga tcgcgccatt 25500
ggagccggcg acgggcttca tgccggtggc agcgcccgcg ttccatcgct tcgcaatgtc 25560
gatggccgcg cgctcttgag gcgtcagcgt cgggttcttg ccggtgaaat acttgtcggc 25620
caggtcatcg gcggccaggg ccagggtggg cgacagcagc gcagcgccca aggcgacaac 25680
ggaaaagatt ttcttcatgg tgaatgtcct tgggttcatt gaacgcgcga ccaggagtag 25740
tcccgcacgt acacgcttag ggggttgttc cgaagttgct catccgtggt ttgcggcgtg 25800
acttccgcga cgtacacagt gaccagcgca cgccagatcg cgggcttgcc cttcggtgtg 25860
ccttgacggt cgcgggcggt ttcttcccac tcgacttgcc aggtgtccgg cgtctgcggg 25920
atggccgtct tgatctcgac gttcaccatt tccttttcag cgcgacggaa cgggctggca 25980
tccggggtgc cgttcaacca ctcattcatc tttggcgttg ccggatcgtt gggcgacaac 26040
ttggcgtaca cgcggaacac agagcggcgc tgcaaggcca cgtccggcgt gaccgtgcgg 26100
gcgtcggtga tgaactcggc gaccgtcgca tggatgacgc gggcatcggc cttcgaggcc 26160
gcttgcaccg ggccggcggc cactgtctgg ccgagcttgt ccacttccac cacgtagggg 26220
atgaacttcg actggctgcc gatgtggatc acgccgccga cgccggcgag cgcgatcaac 26280
agcgacaaga tgccgatcac ttgccaggtc tggcgagacg acacgacgcc gcccacgtga 26340
tcgttccagg tgcgacgagc ggccagatag gggttctcgg cctcgccctg gcgtcggccg 26400
ccttccagcg gttcatcggt ctgccgaggg tcgcgcttgt cgcgtcctgc ggccgtggcg 26460
ggcttcttga agatcaagcc cttgagggtg tcggcaaagc tcatgcggcc tccaggtagt 26520
cattgagatt cagattgcgg ccggccagcc agtgatcgac ccactcatgg ccgaacttcg 26580
cttgcaggct cttgatggcg gcgaccgatt ccttgtcgga cgcgccgacg aaggccaggg 26640
ccagcggccc cagggcgagg tcatagaggc ggcggccgtt ctcggagacg tagtagtact 26700
gccgcttcgg aaccgccgtg gcgaggattt cgatttgccg cttgttgagg cccatgcggc 26760
gatagagcgc cgccgtgtcc tcgtcgcggg catagacgtt cggcaggaaa atcttggtcg 26820
cggtcgattc cacgatcaca tccaagatgc ccgagttggc cgcgtcggaa aggctttgcg 26880
tagccatgag caccaggcag ttcgccttgc gcaagacctt gagccattcc cgaatcttgg 26940
cgcggaacgc cgggtggccc agcatcaacc aggcttcgtc caggatgatg acggccggct 27000
ggcctttaag gccgcgctcg atgcggcgga acaggtacag cagcaccggc agcgcgaact 27060
tctcgccgag gttcatcagc tcttcgatct cgaacaccgt gaagtcggag agcgacaggc 27120
catcctcttc ggcatcgagc aggtggccca tcgagccgtc caccgtgtat tgcttgatgg 27180
cctcgcggat cgcttcgtcc tggatcgtca ccgagaactc ggagagcgtg cgcgcgccgc 27240
tggcgtgcat gctcatgatc gcgtggccga tctcgttgcg ctgccctggc gtggtgtcca 27300
cgccgttgag cgcaaggatc gtgtcgatcc actccatcgc ccaagcccga tccgccttcg 27360
tttcgaggaa ctggagcggg cagaaggcca ggcggtcatc gtcggcggcc accgtgaagt 27420
gcaggccagt cttgcccttc gagccggcat tgattgcggc cgcgagcggg tacatcgaca 27480
tgcccttgtc gaaggcgtag atcgacatgc cggcatagcg ccggagctgc gcggcgatga 27540
tgcccaggtg cgtcgatttg cccgcgccgg tcgggccgaa catgaaggtg tggcccaggt 27600
cgcgcacgtg caggttcagc cggaagggcg tagcgccgac cgtgacgcag tgcatgagcg 27660
ccggggccag cggcgggtac atcgggcacg gcgcggtggc gctgcccgtc cagatggagc 27720
ttgtcggcag caggtcggcc aggttcatcg tgttgatgag cggccggcgc acgttctcga 27780
cgccgtggcc gggcaagctg cccaggtagg cgtccatcgt gttgatcgtc tcgatgcgcg 27840
ccgcgaaggc cagccggttg atggccttct cgaccgacag cgcggcggcc gccaggcgct 27900
cccgatcctc atccatcaac acgaccacgg acgtgaagta gccggcggcc accaggccgc 27960
tattgacctc ggcgatggcc gcctcggcat cggccaccat cgttgccgcg tcctggttga 28020
cgctgccggt gttggtgttg aaaacctggt cgaagaagcc gcgaatcttc tgcttccact 28080
tcttgcggaa cttgtccagg tgcttcaagg actcgtgctg atccatgaag atgaagcggc 28140
tcgaccagcg gtattcactc ggcagctcgg ccagggccga cagcaggcca ggcgttgact 28200
ccaaggggaa gccttcgagg gacaccacct ggatgaactt gcggccgatc ttcggaacga 28260
cgccgcccca cagctcttgc ccgccgatca cggcgtccaa gtacatcggg ttgctgggga 28320
gctgcaccgg gtgatagttg ccggtcacgc agaactgcaa ccagctcagg aagtcgtcat 28380
gcgtgacttc cctgccctct tccgtggcga tcttgtggcc cttcaagcgc gtgagcttga 28440
cggccgagga catgcgcgat tccaggctgc ggatttcgcg cttgaactgc tcgatgaggc 28500
ccacggtgcg ggccgtgtgg tcgggcgtca ccgcgtcatc gtcaaacatc agctcaacga 28560
acttgcgctg ggccagcact ggcggaaacc aggtcacggc gaatacgaag tgcccttcgt 28620
acatcgtgcc caggccctcg aagagccgcc ggcgctcttc atcaatggcg gccgacaccg 28680
gatcggtgaa ggtggagacg ccgcgctcgg agtagttggg tgccggccgg cgcacggcat 28740
cgacatgaac catccagccg ctgcccaggc ccgccagggc ctggttgatg cggaaggaca 28800
ccatttcccg ctgctcttcg gtgctgctgg cgttgtcatc cccttggtac agccaggcgg 28860
ccataaagga gccgttcttg cccacgatca cgccgtcatc gaccacggcg gcatagttga 28920
gcaagtcggc caggcccgcg tccttcgacc gatgcttctt gagcttcagc tcggcatcga 28980
cctgacggat acgggcaaag aggatgagca acagaaccgc accgagtaca gcaatgccaa 29040
ttgcgattgc ttcgatcatt tgtattgctt cccttgactc ggcgtgtttt cacggaaggg 29100
cgtgctgcga gccgggtagt acggcttgta ccggcggtgc cgcaggtaca cgtgtcgcag 29160
cttcgggtcg gacttcgcca tgaggcgcag cacgaataac gcgccgaacc acaaggcgat 29220
gccgaacacc gttgccctca cttcttgggc gctgaaaatc agcgccccgg ccagcaggcc 29280
cgagaacatc accagctcgc ggtcgccacc catgaacagg ttttctcggt tgccggccct 29340
acggatgggg atcgcgcgca gtgccatgac ttaccccgcg atgtggccgc gttcggccca 29400
gcgtgcgatg gcttcaccag ggaagccggc catgtccagc gccgcgacct tggcctggtg 29460
cagcacgcca tcggtcaggg cggcgatttc cgcgccacgg ccgaagaagg tgctcatcat 29520
gttctgcgcg ccgaccagca gggccatgac caggacgatg aagatcaacg tgcggaagaa 29580
gccgttgagt tcgccgccga agatcaagat gccgccggcg acaacgatgc cgatgatcga 29640
cagcgcgaag gccaccgggc cggtgacgct gttgcgcagg ttggtcaacc agccttcata 29700
cggcagggaa ccgccggtgc cttcggaggc gaaagcgtgc tggggggcca gcaggaagaa 29760
cgccaagagg gcgaacatgc ccaggtagaa cacggcgcgg ctgtcgaagc ggaacgcggg 29820
aagggtcgat tgcatgggtc taagctcctt acagggttac agggttacag ggttttggtt 29880
gcgtactggc cgttggcgta gccggaaacc tcaaggattt cctggatgcg ccgcgagccg 29940
tcaggggtgc gggcgatatg aacgaccacg tgaaccgcct cgcctatcag cggttcaatg 30000
ggtttgggtg aatcggggtg catgctgatg agcatggcga gccgatcaag gcccgctttg 30060
gcgttgtttg cgtgcagggt tgcggctcct ccttcgtgcc cggtgttcca ggccatcaac 30120
agatcaagtg cttcggggcc gcgcacttcg ccgaccagga tgcggtcggg ccgcatgcgc 30180
agcgtggtct tgagcagcgc cgtcatggtc acgtcgatgc tggtgtggta ctggacgtag 30240
ttctcggcgg cgcactggat ttcgccggtg tcctcgatga tgaaaacccg ttcggtcggg 30300
tcttgaagca ccatctcgtt gatgatcgcg ttcaccagcg tggtcttgcc cgagccggtg 30360
ccgccgatca ccaggatgtt ccggtgggca cgcacggcgg caatcaagga atcgcgctgc 30420
tgcgcggtca tgattccgcg ctcgacgtac tgcgccaggg tgaagatggc gaccgccttc 30480
ttgcggatcg caaaggtcgg tgcgggcacg accgggggga gctggccggc aaagcggcta 30540
ccgtctagcg ggaactcgcc ttccaggatc ggccgctgcc gcgtgacttc cttgccgtgg 30600
tagccggcaa tggtttcgat catggcttgg gcctgcgcga agcgaagggt gccgatgcac 30660
ttcatcggct cgcccagctt ctcctgccag actttgccgt ccgcgttgag catgatttca 30720
acggtcttgg gatcgttcag agctgccaac agctccggcc ccatgtcgcg ttcgagcttc 30780
ttcttcgccc gttccttgat cgtgttgtgt tccagcttct gttcggtcat tccgtgtatc 30840
ccatgtatgt gatttgcgtg ctgccctgac cgcacgcatt ctacacaggt tcatacgata 30900
taccgaagtc ttttgacgag caagcaaacg gaatgacgga ttgagtgttt tcaatcgtgt 30960
cccgagctga tcgcaataga aacaacgcgg cgcatgctcc gtgcgcctcg caccgaacct 31020
atggcttgcg ttgtgggtgt tccgggggtt gagccacccc ggcgggcttt agcggctaaa 31080
aagtgctggc ttctgctggc tcagttcctt gccgcgtccc ttccctgctt tctttcttgg 31140
cgtcctaccc ccggagcttc ttgcgggcgg cctcgcccca cttcttgacg atgaatgcct 31200
gatgcgacgg tagcaccgcc gccactcgct cgaaaccggc aggaaggctc tgcggagcct 31260
tgccgccggc cagggcatcg agcgtgtcct tgtccaagtc ggttgattca agcagaaggg 31320
gcaacggcgc atcaagggcc ttggcaatgt cctgcatgac cttgagggac ggattggcct 31380
tgcccgtagt gagatcggac aggaaggaga tcgaaacccc cgacctgtcc gatagctcct 31440
ttttcgtcat gtggcgctca tcaagcagcc gcagcacgtt cgtgaagaag atgtagttat 31500
acaaaggcgt cgccttgcgc atgggcggct cgaaatttta gccgctaaag ttcttgacct 31560
cggaattagg tttagctaaa ctctaggctt catcacagcc tggagagcta cgcatgagcc 31620
ataaccaatt ccagtttatc ggcaatctta cccgtgatac tgatgttcgc catagcgaaa 31680
acagcgcccg cgccgtattc gacctggccg tgaaccgcgt gtggcgcgac cgcgccggcg 31740
agaagaaaga gcaaaccgac ttcttccgca tcaagtcgtt tgccggcctg gcagagaacg 31800
cgggcaagta ccttggcaaa ggctccaagg tgttcgtcca gggccgcatc gagccgacga 31860
agttcgagaa ggacggccag accgagtacg gctttgactt catcgccgac gtgatcgagt 31920
acctggatac caaggaacca ggcggcaaat gagaaacgcg cccggccaca ggccgggcgt 31980
tatgctagga ggacgaagat atgccggaca gtttcgacga gcagggctac cgccgcaagc 32040
tcatcgacgg cgggttgagt gaggccgacg cgacggcgct tgcccgccgc tacgcggccg 32100
agcgcaacgc gcaggccggc accgccggcg gggctttccg ccccgccggt tccctcttcg 32160
agctgccgga cgggccggca gcgccggagc caccgccacc gccgcccagc gcggccgtgg 32220
cgcgctccga gcggatggcg gccgagtcgg ccgagctggc gcggactatg ggccttccgc 32280
ccgagccgcg aaccgtggtc aacaccttgg ccgagaaagt cagcggcgtg accgccgaac 32340
ggagaacgca acagatggcg accaagaaaa cgaccgagaa gccggccagt gccaccggcg 32400
agctggccga gcaggtcagc caggccaagc aggccgcctt gctcaagcac acgaagcagc 32460
agatcaagga catgcagctt tccttgttcg acctcgcgcc gtggcccgac cacatgcggg 32520
ccatgcccaa cgacttcggc cggtcggcga tcttcacgac caggaacaag aaggtgcccc 32580
gcgccgcctt gcagcgcgag gcgatctacc acatcagcaa ggacgtggag atcaccttta 32640
ccggcatcga gctacgcgcc gacagcgacg agctggtgtt tgcccaggtg ctcgaatacg 32700
cgaagcgcac gccgctgggc gagccggtca cgttcacgtt ctacgagatt tgccaggact 32760
tgggctggtc gatcaatggc cgctactaca agcaggccga ggactgcctt tcgcggctgc 32820
aagcgtccgc gatgcagttc tcttcccagc ggctgggccg cctggaatcc gtgtcgctga 32880
tccggcgctt ccgcatcctg gatcgcggca agcgcacgtc gcgctgccag gtcgagatcg 32940
acaccgaaat ggtggtgctg ttcgccggcg accactacac gaaattcgtg tgggagaagt 33000
accgcaagct gtcgcccacc gcgcggcgga tgttcgacta ctttgccacg cacaaggagc 33060
cgtacccgct caagctggag acgttccggc tcatgtgcgg ttcggattcc acccgcgtga 33120
agaagtggcg cgagcaggtc agcgaggcat gcgacgagct gcgcgaaaac ggcctggtgg 33180
acagcgcctg gatcaatgac gacctggtgc attgcaagcg atgaccgccg ccctgcccct 33240
ggagagccgc ccgagatcga ggcggctttt tttctcgagc atcgccgctt ctgacgaggg 33300
agttccggcc acctggtcgc gcgcacgtcg agctgcagca tcgtcatttc cggcgaggct 33360
ggccacgtcc aggccacggt cgaccatcgc cacctttgac gatgaaccga gggccccatc 33420
gccagttccg acgatgacgg ccgacgccgg cgctggatct cgcatcgccg cttctgacga 33480
tgagatccac cctttgacac ttgaggggcc gagctgcggg ggattctccc ggccggcggc 33540
cgcgagaacc ggctatgtcg tgctgcgcat cgagccaggc ggcagagccg cccgtctcgc 33600
ccctggcggg cttccagcgc cttcccgacg ctcaccgggc cggtggccct cgccgctggg 33660
ctttgcggcc ctgacgggcc ttcctggccc gtttccgggg ccttccctgc cctgccccac 33720
cggcgcggcc gttgtggaca gtttcgggac aagtcggccg aggccggccg gcgcagccct 33780
gacgttgaca cttgaggggc agcggggccg gcgcgatgtt gacacttggg gggcagcggc 33840
cgatttcggc ctggtcgagc cgtccaggcg gccgccgagg cggcctaagt gcctgatcgg 33900
catggagtta tccaccgaag ttgtggataa ccctgttagg aaccctgccc cggtattgac 33960
acttgagggg cggccctact gacacttgag gggcgcggtt cttgacactt gaggggcagc 34020
gtattgacac atggggggct tatccattga cacttgaggg gcggcctctt cgcgcttttc 34080
cttggcgtga caacggttta cgcgcgtttt tcggtcatgc taaccaggtt tttaaccgtc 34140
tttataacct ttttatataa ccaggtagtt ttaaccagag gctggcgaaa aaaacggcaa 34200
cacggcccaa gctaccgcgc agcatgaccg gccgccgaag gggggaaacc ccccttctcg 34260
atcccacccg gccgcaagcg gcctcccatc cccccagggg ctacgcccct cggccgcctt 34320
gcggcctcac cccacaatgg agcgccttcg gcgctacggc ttaggagagc caggcgcaag 34380
cgcctggcgg aaggcagtaa gttcgagagc ttgcgagggg gcaaagcccc ctcacactcc 34440
ccaaccaggt cgccgcggtt cgaccggccg cgcgatcctc aagcatcgca gcagctccac 34500
cgccgcaggt tttacgccaa ggaagggggt cggccagctc ggccggtggc gcggaaagcg 34560
cccctcaagt gtcaatgaaa cgtgagacgc ggggccaggc ccgcgccgat cacaagccga 34620
gttcggagtg agagccgagg cgcaccaggc gcagcgtgtc ggcgtcgggc ttctggtaga 34680
tcagcaccag gtcgggcttg acgtggcaat cccggtgatc cttccagtcc ccggtcagcg 34740
cgtggtcgcg gtgccgaggt tccagcggct ggtcgtcggc gagcgcgacc aggacgggca 34800
ccaggtcggc gtcgagcgtg gcccggtgct gccctttcgc ctcgcgcttg tagtcgcgct 34860
tgaactggcc ggtgcgctca atctgccgca tgcagatcgg ccatcagatc gttgacggtg 34920
gcgaagcgcg gcagcttgcc cttgcgggct tccttcatcg cctcgatggt ggtggcgttc 34980
ggaatcagcg gctcgaaggg cagcgccttg tcctgggcca ccttcgtgag cagcagccgc 35040
accgcgtcgg acacggtaag gcccatcgcg gccaggacgg ccgccgcttc ttccttgatc 35100
gcgccgtcga tgcgcgtttg tacgagctgg tttgcagcca tgattggttc tccggttggg 35160
ttcgtgaatt acattgtaat gcacgccaag ggaaagggca aggtttagcg gctaaaacgt 35220
ttagctaaag tgcttgacga ttaggacatt ttgccctaat atagcacttg aaggccgggc 35280
atttcgccca ggtcaactac cggagaaagt ccgatgaaca cccaagagca acccgttacc 35340
gcttccctgg tcgccgaggc ccagcgcctc gacttcctgc ccgcctactt cggcccgcgt 35400
ctgatgatgc gcggcgaggc cctggtgtat gcctggcttc gccggctctg cgaacgctac 35460
agcggcgcgt attggcacta ctacaccttg tcggacggcg gtttttacat ggcccccgac 35520
ctggccgacc gcctggagat cgaggtggac ggcaacggct tccgaggcga gttgtcggcc 35580
gacgccgccg gcattgtcgc aaccctgttc gcgctgggtc agctcgcggc cgaggccgcc 35640
gacacggacg ccggcgatgc cctgatcgac cgctatcact tcctgcgcgg cttcgcagcc 35700
agccacgccg aggcggccgc gatctaccgg gctattgact gatgaggggc ggccccggct 35760
tcggccgggg cttccaggag gcgaccatga ccaggaacaa cgctccccag gcaccgacga 35820
acagctacgc gacggacggc aagtgccaca acgccgagcc gggcacctac ggccacgaat 35880
gcggcaagcc ggccgcatgg atcggcacga accacaaggg gttcagctcg ggattctgcg 35940
acgactgcaa gcgccacggc tacgaagccc gcgacgtgat cgcgtggcgg cgtgctgaca 36000
cttgaggggc cggccgatgg cgaagaaacg caagacgccg gcccagccgg gccaggactg 36060
gaacgcgccg gcggccgagc tgctggccga gctgcccacc gaccgcgacg agctgctggc 36120
cgccgccgtg gcggccgtgg tggagatcga cgcggcgatc atgcgcggtg acggtgcggc 36180
cgccgaggcg gccagcgacc gatacgaggc cgtcatctgg aagatgaacg gcggaacgca 36240
cgtgggcagc atggccgacc acgacgcgcc cggccaggtc atcgagcgcc attgcgcggc 36300
cggtgcctgg cgacgtgccc ttgtggggcc agcgcggcca gttcctggtc gcggatggcg 36360
acatgcgcgc cctggtggag tacgaggccg gctacggcgg gccgctgggt gcccacttcc 36420
agttccacgt catcgacctg gacaggccgt ttatctccga aacgggctac cgctcgcact 36480
tcgacacggc gcagggctgc atgacggtgg acgaggttgc gcgcggcatc ctggctgcgc 36540
agcgagccga gaagaagcgc ccggtgatgg tcgaggccag ctaccgcgac cgcctggccg 36600
atgctcccct gcccgcttgg ctggccggct tggagccgcc ggcgcggcgc gcaccggcga 36660
ccgtcctggt gccgccgggg ttcgtcctgg tcgatgtggt gctgccctcg catcgggcct 36720
tcatcgccag gcgctgggcg gccgaggccg cgaccaggat caaggcggcc agggccgccg 36780
ggttggacgc caagggaaaa atcaccggcg caggcccgga gcgagatccc gcatcgccag 36840
aaacgacgat gacctgcagc atcgtcaaag ccgacgatgc cgagccggtc gagggcgggc 36900
ctgtatcgcc ggaaacgacg atgccgggag cgagcgcggc aacctgcagc atcgccagga 36960
atggcgatgg aggcccggcc gaccgggtgg agttcaagcc cggccagcgg tgcgagatcg 37020
tgagcgtcca tcacccggtt ttcgaccgct acatcggcaa gcgcatcatc atcgtgaagg 37080
tgcatcccga cacccgccag gtgtgggcgc acgacgacag gccggtaacg tacaagacca 37140
atcgcgcagg ccggcgcgtg gtcgattcag acccgagctg catccagtcg atctatggct 37200
tcgaccagtt gcggcttatc acttgaccag gagaacgaac gaatgacgaa cgacgccaat 37260
atccggctgg agtgcttgaa gccggccgaa cgctgggcgc agccgaccgg ggaggaagtc 37320
cgcgaggtgc tgcgcctggc cgggttcagc ggcagcaagg ccgcgaaggc gctggggctg 37380
ggcgcggcgg gcgaccgcac gatccgccgc tggatcggcg aggacacccc gattccctat 37440
gcggcatggg cgatcttgtg cgacctggcc gggctgggcg tgatctggaa agaggattga 37500
cgccaaggga aaggttttag cggctaaagt ttagccttcg gattccggta aaacgaaaaa 37560
agtttagcta aagtgcttga caggctaggg cattttgccc taatatagca cttgaaggcc 37620
gggcatatcg cccaggtcaa ctaccggaga aagtccgatg agcgacaaca agatcatgcc 37680
ctggattgac gagctggggg gcgcggcagc gaccgacttc ccggcccgcc gtgacgagat 37740
cgcggccatg atggccgagg cggccgagct ggtgcgcaag gccgaggaac tgcgcggcaa 37800
ggcgtacttc gccgggtgct ccctggaagg ccaggccaag ggccattggt cgatggaagc 37860
ggtcgagcag gccaagcgcc gggtcggctg gtaagagcac atggcccggt cggcgacggc 37920
cgggctgatc gaggacgcca agaaggagta ttgcgtgagc cgaggcaaga tcgagggcgg 37980
ccccaggtgg aggaacgccc cggagtgatc cggggcgctt ggcctggtgc tggcacaccg 38040
taaccgtgct tccgagtggg agcacccagc attcaacacg taagcgcgtg gagggtcaag 38100
ctatgtccaa catcatcaag tttcccaagg agatcgagca gcccgccgcg cccgcgccgg 38160
tggagcctgc cgctgcgcct gctgcccctg cgaaggcccc aggtgctggc ctgctggccg 38220
gcctggtcaa gttcgtatgg gtggcgaccg tgctggtctg gccggtgctc aagtgggtgc 38280
tgtcgatcat caccttcttc cagttcgtgc ggatgctcta ccactggaac acgcccggcg 38340
tgtatgccgg ctggtctttc ctggcgtact tcgcggcact gtccgcgatc acctacttcg 38400
tttcgattta caagccgaag gggctttgac atgaagaagc agaccatgcc ctacccgccg 38460
gggttcgtgg agccgaacac cggccgggtg gccgtcctgg tgcgcgacta cgcggccagc 38520
gacttgaacg gcgatgcgcc ggcctactgg tacagcgcgc aatccgaaga atggggcctt 38580
gacccgtggc ggctggtgga aggcgtcgat ccgcacacgt ccggcgggca gttcgacgtg 38640
tgctttgcca gcggatcgag tcgcacggtc ggcccgctga tgacgttctt cttgagtgcc 38700
gctgacgcgg ccaggttgaa cgccaaggaa ggagatcacg cgcccatttt tagccgctaa 38760
aaggcttgac gcgggcggca agtttagcta aacttctgca catggtcggc ggctggcacc 38820
gccttttgtt caacaaccgg gtgtggagtt tatatgggtg ttctccatga agaaacggct 38880
aaccgaagcc caattccagg cggcgatcaa gggcctggag atcgggcaac agaccatcga 38940
catagcgcgc ggcgtgctgg tcgaggggag gccccaggcc gagtttgtgg cctcgctggg 39000
gctgtccaag ggggcggtat cgcaagcggt cagtcgcgtg tgggcagcag cgggggaagt 39060
gctccccgag ggcttcgagc gggtgacggc ggtactgccg gagcatcaag cgttcatcgt 39120
caagcgttgg gaagccgacg ccaagggaaa gaggaaacag gaacccaact catgaaaaca 39180
ctggtcacgg caattcagaa aggcggtcaa ggcaagacgt tcgcaacctg ccacctggcg 39240
ttcgacttcc aggagcgcgg ccttcgggtt gcagtgatcg acctggacac ccagggcaat 39300
gcgagctgga cgctggccgg ccacgactcg ggctatcccg ccagtcgcat gttcaccgcc 39360
ggcggcgacg agctgcgcgc ctggttcgct gaccgggaag atgacggcct ggcgctgatc 39420
gcggccgacg ccagcctggc gaatctggac aagatggact tggcccaggc cgccggtgcg 39480
ctgcgggcca gcatcgaggc gctgggcgag ttcttcgacg tgtgcctgat cgacaccgcc 39540
ccttcccttg gcgtcgccat gacggcggcc gtgctggccg ccgactacat gctttcgccg 39600
gtcgaaatgg aggcgtacag cttgcagggc atgaagaaga tggttgccgt catcggcaac 39660
ctgcgcaagc agaacccgaa gctgcgcttc ctcgggatgg tgcccaacaa ggtggacgcg 39720
cggaagccgc gccatgtcag caacctggcg accttgcagc aggcataccc gcaactgatc 39780
ttgccgttca gtgtcggcgc gcgagacagc atcgcggagg cgctgggcga gcagatgccg 39840
gtgtggaaga tcaagaaaac cgccgcgcgc aaggccacgc aggaagtgcg cgccctggcg 39900
gattacgtgt acacgaagat ggagatcgcg caatgaccag cgccgtaaag accaacgcca 39960
agaagaagac cccgcaagcg gccgcagaga agcccaaggg cggcgggctg ggcctggatg 40020
gcctcggcga cctgtcgagc ctgctggacg agccacaggc cgcgaatgcg gcccctggcg 40080
gcggccccca ggagttgccg cttgacctga tcgacgaaga tccacaccag ccacgcacgg 40140
ccgataatcc cggcttctcg ccggagtcca tcgccgagat cggcgagacg atcaaggcgc 40200
gcggcgtgaa gtcgcccatc agcgtgcgcg agaacccgga cgcaccgggc cgctacctca 40260
tcaaccacgg cgcacggcgc taccgtggct cgaagtgggc cgaaaagagc accatcccgg 40320
ccttcatcga caacgactac aacgaggccg accaggtaat cgagaacctg caacgcaacg 40380
agctgacggc ccgcgagatc gcggacttca tcggccgcga gctggcgaag ggcaagaaga 40440
agtcggagat cgccaaggag atcggcaagt cgccggcatt cgtcacgcag cacgtcacgc 40500
tgctggactt gccggagccg attgccaagg cgttcaacgc tggtcgcagc aaggacgtga 40560
ccgttatcaa cgagcttgcc acggcgtaca agaagaaccc cgaggaagtg cgcgtatggc 40620
tggaggacga cagccaggag ctgacgcgcg gatcggtcaa gatgctgcgc gagtacctgg 40680
aggacaagcg cagccaggac ggcgagcgcg accccaacac cgtcgatgcc ttcaccggca 40740
agacggacgg cgaggccgag ggcgacggcg acgggcaagg cccggatgat gacgccaaga 40800
agaaggagcc gaaggaaccc gacccggaca agctcaagaa ggcgatcatc caggtcaagc 40860
acgatgaccg cccggcccgc ctgatcctca accgccggcc gccggccgag ggctgggcct 40920
ggctcaagta cgaggatgac ggccaggagt tcgaggccga ccttggcacc gtgcagcttg 40980
tcgcgctgct ggagggctag acgcccctgc accagacccc gccgaccccc ccgcacccct 41040
gcgccccgcc atcgagcggg gctttttttt tgcctgcgcg cgccgcgccg ggctggccgc 41100
gttttagccg ctaaaaaagt tcagctaaac ccttgcgccc tgttgtatgt tgtagtatgc 41160
tacacgctac aacatacaac accggccaaa aggaggccca atcatggcaa tcacgaaaga 41220
gcagatattt gcggtcgccg acgacctgga cgccgccggc cagaacccca ccctggcgaa 41280
cgtgcgcaag cagctcggcg gcggaagctt cacgaccatt tccgaggcga tgaacgaatg 41340
gcgggcgcgc aaggccagcc aggcggcccc gatccgcgag ccggccccgg cttcgatcac 41400
cgagaagctg gccgagctgg gcggcgactt gtgggccgtg gccctagaca tggcgaacaa 41460
ccggctggcc gccgagcgcg aggcgctgga agctgtccgc caggaaaccg aggcagcgcg 41520
ccaggaggcc gcagagctgg ccgaccagct caccgtcgag ctggacgagg ccaagggccg 41580
tatcgcggcg ctggaggccg cagaagccac cgccaagggc gaggccgagg cgctacgcga 41640
gaagctggca gcggccggcg agaaggcggc cacggccgag gcccgcgccg gcgagctgcg 41700
cacggagctg gatcacgccc accaggaagc gcgccaggtg cgcagcgagc gcgacaaggc 41760
ccaggagaag gcggcggcca gcgccgacca ggcggaggcc ctgcgggccg acctggcggc 41820
cgcgaacagc cgggcaacgg agatcgagcg ccgggccggc gagctgcgcg ccgacctgga 41880
gcgagccaac caggccaccg accaggcgcg ggcggcgctg gccgagcagc agaaggccac 41940
cgaggcggcg gccgggcagc ttgaccaggt gcggggcgag ctggcaaagg tgcaggccaa 42000
ggccgaagcc gagcgcgagg cgcaccagga gcagcgcaag gcagcagcgg ccgaagcgca 42060
ccgccaggcc gaacgcttga cggccaccca ggccgagcgc gatgccgcgc gcaaggaggc 42120
cagccaggcc cgcgaggacg ccgcgaccct gcgcggccgg ctcgaagccc tggaaaccgt 42180
catggcccag gagaagggca agacgggcgg cgcgaaaaag acctgatatt tcggtcggtt 42240
ttcaataaaa tcgagggtgt aacctggaac tacacatccg gccacgagag gaaagccggc 42300
ctcgcgcatg ccgaggtcgg cttttttctt gctggactgg caaggaggcc catctattcc 42360
acattttgaa atgaccacac cgacgataag gagcgcccga tgaaacagcg cctcttggtc 42420
atgaacggcc aacgcatcgt tcagaccgag caagaagggg catgggccaa ccagaaggtt 42480
gacaaggctg gcgcattgaa gcccggcatc tacaacctgt acatggccca gcaggccgac 42540
aagaagcaga cccatgacgg cgtgatcgtt cacgccgaca acaaccaggt gtaccagcag 42600
gtaggcaaga actttgtcat gcacgcccgg tcagatttcg ataaagtacc tgaaatcggg 42660
agcgcaaaga gcatcagcta cagcgcgcaa ggcaaagcaa ccgttgccgc cgaggccccg 42720
aaactcacgc gaggccggtc ccgatagctt ttagcggcta aaaggagggc cacgccttga 42780
gacgactcac acccaccgcc ggccgggcag acgcccgccg catccagccg gcatccgccg 42840
gcttcgcaac aagcacgacc agcagaaccg acgccgccgg cgacggcctg ctggccgctg 42900
ccgaagccac agaggccgag cagcaagcgg ccctggaggc cgccccgctc gaccagacct 42960
accaggaagc ccttgccctg tacgttcagg ccaagcacga ccaggtggag cgcatcgagg 43020
atcggcttga aaacctgatc gaccgtcagc aggcgcgctt gcagcagacc caggccaacc 43080
agccgggcct actgtcccgg cctggcgcga aacgcgcttg gcagaaccag cagatgcagc 43140
agcaggcgcg cttgcagtcc ctgcacgtgc gcctggaagc cgtccgcgag atcaaggaag 43200
gcatgggcct gcactcaccg aaggtcgaag agctggcgac ccgcaagatg cgcgccgaga 43260
aacccgagct ggcggccgat tgggatgcca tgcgcgaggc ggcacgccgg caccaggcgc 43320
tgcaacgccg cgaagagcag gagcgaaagc aggcgcaggc cctggagcga ccaggccggt 43380
cgcagagctt gggcctcacg cggcccgtct aaaacgaaag cccggcactg ccgggctttt 43440
tcctcgagcc tcgccgtttc cggcgatgca ggcccgccgc tggcggcatc ctgcagcacc 43500
gtcattcccg acgatgcaag gggctggcca gcctcgccag ttccggcgat gccgccggcg 43560
cggtcgacca tcgccatttc tgacgatgag atcccgcatc gccatctaca gcgatgccca 43620
caacgcgagg gcggccgcga aaaccgccat gccggccgcc acgatgttca tgtacgacac 43680
gcgccgcgct tcgcggatcg gcgcggcgag ctgcgcggcg gccgcttcca gctcgcgccg 43740
caccgcctcg gcggcggcct tgccgccgtc ctgcatcccc ttcgccatcg cctctttgga 43800
ggccgccagg gccgcgttga gcgtcctttc ggccttcccc ttggcgtcat ccccccaacg 43860
gtgtgcgatg gcttccagct cttccttgaa gcgatccagg atttcttgct gcgcggcctg 43920
gctgtcctgc atcagtcggg tgttgatcgt ctggaggatc aagatcggat cgtcgcggcc 43980
aacggcgatg ccgtgcttga ccgcgatttc ccgtatcagc tcttcgatct tgtcatcgct 44040
cgccatcaga gcaccgccgc gttgtcgagc tgctggaaga gttgcgactt cacgatcttg 44100
agccgctgcc gcgtcatgat cgtgagcgcc gagtcggcca gggcctcatc gaacgtccgg 44160
cgctcctgga gcatttccga gaagtcgcgg ccgtaggttt cttccttgag cgccggaatc 44220
tggatgatgg ccgacacccg cgccttgttg tcggtgtacg ccttcatctt ctcgaagccc 44280
ttgccttcgt gctcgattgg cccccaatac gggttcagcc acaccacgaa cacgcattcg 44340
gccgggaatt gccgggccag cgtgctaaag ccgttcaggg tgtccaggaa ggcttgcccg 44400
ccggtgacga ccgtatgcac caccagttcg tgccccatgt cctggagcag cgccggaacc 44460
tggttgccga tcaagtagct ggacagcggc acgaacgagc tggccccgtt gtcgatgatg 44520
acgtcgccct tggtcgaggc gatttgctcc accagggcat cgaagttccg ggtgttgatc 44580
tcgtcgccgt ccatgatgtt caggcgctgc acgttcaggg ccttgtaacc ctcaaacgtg 44640
ctgttgaccg gatcggtgtc gatgcaaagc ggcgtctgcc ccttgctcgc tttgtactgc 44700
gcaatggtgg ccgcgatcat cgacttgccg acgccgccct tgccctgcaa gaccatgtga 44760
attttcgcca ttacagtaaa tcctctttat tcggtttcgg gttgtagttg aagccggcga 44820
tccctgccga ctcgggtttc ttcggctcgg gtgccttcgg cttcctcgcc ttcgaccccc 44880
cttcctgttg cgccggcttc tcccctttgg cctggttggc cgccggggga ggcaccggcg 44940
cgggtgctgc tggtgcggcc ttgatgtacc gctggacgtg cttgcggaac gtctcgtagc 45000
tgcacttgac ccggccgatc tcgcgcatgt cctcgtagat cgtcgtcact gcatagccag 45060
catccatcgc ggctttcacg tccacgcgca cggccaggaa tgcaacggcc gtcgcgtcct 45120
gtcggcgctt cttggcggcc cgtttctcga tccgcgcggc cagctcttgg gggtagctgc 45180
tttctgccat cgcctcgctt ccgtggtttg taaatagccg tcattttcat gtatcgaccc 45240
gtggaaagtc tatgtaaagc gcgtggatag cagcttgaca ccgctgcatc tagcgcgctt 45300
tccgggcatt gtacgccttc attcgatata tccgccatct tttcgcgcta aatgcagaca 45360
gatgcgaagg tttcggtata gactttcctt ggtgttatca acgccgtaag gcgggcagga 45420
tgtgcgaagt tagctaaccg gcgggccggt tatctatctt cgctgtccct tattcgcgcc 45480
tgcggcactc aacgggcatc ctgctctgcg aggctgccgg ctaccgccgg ccaagaagcg 45540
agagacgaac gacatgggct acatcaatcc gctgctggag cttccagcag gccgcgaact 45600
ccaggcgctt ccggttgccg accggcagcg ccttgcccgc gtgctgcgcg agctgcgcac 45660
gcaggccaac gacgaggcgg aaaaggcatg ggcacgccgc aaggggccga tggccgccta 45720
ctggcgagcc gtggcgacct acgcgcgaca cacggcgcac gcgctcaagg ggtagcgaca 45780
tggaagccga cgaacagccg accgcgaagc ggcggcagca cctacgggtg ccggtgttcc 45840
ccgacgagaa ggaacagatc gaggcgaacg ccaagagggc cggggtcagc gtggcgcgct 45900
acctgcgcga cgtgggccag ggctaccaga tcaggggcgt catggattac gagtacgtgc 45960
gcgagctggt gcgggtgaat ggcgacctcg gccggctggg cggtttgctc aagctatggc 46020
tcaccgacga cccgcgcacg gcccgcttcg gagacgccac gatccttgcc ctgctgggcc 46080
ggatcgaagc cacccaggac gaaatgagcc gactcatgaa gtccgtggtg cagccgaggg 46140
ccgagccttg agctttttag cggctaaaaa gccaggggct ggccgatgat cgccaagcac 46200
gtacccatgc gctcgctggg caagtccgac ttcgccggcc tggtgaacta cgtcaccgac 46260
gcgcagagca aggatcaccg gctgggccag gtgcagatca cgaactgcga tgccgtgtcc 46320
gtgcaggacg ccatcaccga ggttctggcg acccagcaca cgaacacgcg ggccaagggc 46380
gacaagacct atcacctgat cgtcagcttc cgggccggcg agcagcccag cgccgacacc 46440
ctgcgggcga ttgaggaacg tatctgcgcc gggctgggtt acggcgaaca ccagcgcgtc 46500
agtgccgttc acaacgacac cgacaacctg cacatccaca tcgccatcaa caagattcac 46560
ccgacccgga acacgatgca cgagccgtac tactcgcatc gggcgctggc cgagctttgc 46620
acggccctgg agcgcgacta cgggctggag cgggacaacc acgaaccacg ccagcgcggt 46680
gccgagggca gggcggccga catggagcag cacgcgggcg tggaaagcct ggtcggctgg 46740
atcaagcggg aatgcctgga cgagatcaag ggcgcgcagt cctggcagga gctgcaccag 46800
gtcatgcgcg acaacggcct ggaactgcgc gagcgtgcga acggcctggt gatcgaggcc 46860
ggcgacggaa cgatggtcaa ggcgagcacc ctagcccgcg acctttccaa gccgaagctg 46920
gaggcgcggc ttggcccgtt cgaggcttcg cccgaacggc agacacgaac caaagcgaag 46980
cggcagtacc agaaagaccc gatccgcttg cgcgtgaaca cggtcgagct gtacgcccgg 47040
tacaaggccg agcagcagac cttgaccgcg acacgtgggc aggcgctgga gcaggcccgc 47100
cggcgcaagg atcggctggt cgaggccgcg aagcgttccg gccgcctgcg ccgcgcgacg 47160
atcaaggtga tcggcgagag cggcccgaac aagaagctgc tgtatgccca ggccagcaag 47220
gccctgcgcg acgaaatcca ggcgatcaac aagcagtacc agcaagaccg ccaggccctc 47280
tacgacagcc acagccgccg cacgtgggcc gactggctca agaaggaagc gacccacggc 47340
aacgcggagg cgttgaccgc cctgcgcgcc cgcgaggcgg cgcagggctt gaaaggcaac 47400
accatccagg gccggggcga tccgcgcccc ggccatgccc cggtgacgga caacatcacc 47460
aagaagggca ccatcatctt ccgcgccggc ctgacggccg ttcgggacga tggcgacaag 47520
ctgcaagtct cgcgcgaggc cacgcgcgag ggcttgcaag aagctctacg gctggcgatg 47580
gaacgctacg gcagccgcat caccgtcaac ggcaccacgc agtttcaggc gcagatcatt 47640
cgcgcggccg ttgattccca gctacccatc acgttcgccg atccggccct ggagagccgg 47700
cggcttgcgc tattgaagaa ggagaacacc catgagcgac ccgaccgaac cgagcgagcc 47760
gaccagcgac ccgcagagca tcgaggacga gctggacgcg gccctggcgg ccctggacag 47820
cgcgccgcta cccacgacga cgctgcccga cccatcgccg tcgcccgagc aggcttcggg 47880
cgaccaggtg gcgggcgagc cggcaccgcc ggcgttcacg ccggagccgt ccaccggaag 47940
cccgacattg gacgcattgg acgagtcccg ccgccccaaa gccagcaccg tttgcgaaca 48000
ctgtcccaac tcggtgtggt tcgcatcgcc ggcggaagtg aagtgctatt gccgcgtgat 48060
gttcctcgtc acctggagca gcaaggaacc caaccagatc accaactgcg acgggatatt 48120
tctcggccag gatcaggagt agcagcggcc ccgccgggcc tcgcggcggc ggataaatac 48180
atcgccgaac gcgagtcgaa acgctcgaaa ggtttcgata taccgaagca cgcgcgatat 48240
actgccggtg aaggtggcct tgctttcgag ggtgtccgca acatcgaggg ccaggcgctg 48300
gccctgttga aacgaggcga tgaggtcatg gtgatgccca ttgaccaggc cacggcccgg 48360
cgcatgtcgc gcatcgcggt cggcgatgcc gtgacgatca cgccaaaagg ctccatcaaa 48420
acgtcgaaag gtaggagtag atgaaaataa agatgaacaa cgccgtgggg ccgcaggttc 48480
gcagcgcgaa gccgaagccc agcaagttgc tgccgatcct cggcggggtg tccctggtcg 48540
gcggcctcca ggccgcgaca cagttcttcg cctacacgtt ccagtatcac gcgagcctcg 48600
gggccaacct cgggcacgtg tacgcgccct ggtcgatcct gaactggtcg gcgaagtggt 48660
acagccagta ccccgacgag atcatgcggg ccggcagcat cggcatggtg actgccaccg 48720
tgggcctgct gggtgtggcc gtggcgaaag tcgtcacgtc caacagctcg aaggcgaacg 48780
aatacctgca cggttcggcc cgctgggccg aaaagaagga cattcaggcg gccggcctgc 48840
tgccgcgcga gcggaacgtc ctggagatcg tgaccggcaa ggacgcaccc accgccaccg 48900
gcgtctatgt cggcggctgg caggacaagg acggcaattt ctactacctg cggcacagcg 48960
gccccgagca cgtgctgacc tacgcgccga cgcgaagcgg caagggcgtc ggcctggtgg 49020
tgccgacgct gctttcgtgg ggcgcaagca gcgtcatcac cgacttgaag ggcgagttgt 49080
gggcgctgac cgccggctgg cggcagaagc acgcgaagaa caaggtgctg cgcttcgagc 49140
cggccagcag ctcgggcggc gtgtgctgga acccgctgga cgaaatccgc gtcggcaccg 49200
aggccgaagt cggcgacgtg cagaacctag caactttgat agttgacccg gacggcaagg 49260
gcctggattc ccactggcaa aagaccgcct tcgcgctcct ggtcggcgtc atcctgcacg 49320
cgctctacaa ggccaggaac gacggcggca cggccacgct gccatcggtc gatgccatgc 49380
tggccgaccc gaaccgggac attggcgagc tatggatgga aatgaccacc tacgggcacg 49440
tggacggtca gaaccaccac gccatcggat cggccgcccg cgacatgatg gatcggccag 49500
aggaagaagc cggatcggtg ctgtccacgg ccaaatccta tttggccctg taccgcgatc 49560
cagtggtggc gcgcagcgtg agccggtcgg acttccgcat caaagacctg atgcactccg 49620
acgacccggt gagcctgtac atcgtcacgc agcccaacga caaggcccgc ctgcggccgc 49680
tggtgcgcgt catggtgaac atgatcgtgc gcctgctggc cgacaagatg gacttcgaga 49740
acggccggcc ggtggcccac tacaagcacc ggctgctgat gatgctggac gagttcccga 49800
gcctcgggaa gctcgaaatc atgcaagagt cgctggcctt cgtcgccggc tacggcatca 49860
agtgctacct gatttgccag gacattaacc agctccgaag ccgcgagacg ggctacggcc 49920
acgacgaaag catcacgtcg aactgccacg tgcagaacgc ctacccgccc aaccgcgtcg 49980
agacagccga acacctgtcc cgactgaccg ggcagaccac cgtggtgaag gagcagatca 50040
cgaccagcgg ccgccgcacg gcggcgatgc tgggccaggt gtcgcgcacc taccaggaag 50100
tgcagcgccc cttgctgacg cccgatgaat gcctgcggat gccggggccg aagaagaacg 50160
acaagggcga gatcgaggaa gccggcgaca tggtgatcta cgtcgcgggc taccccgcca 50220
tctacggcaa gcagccgctg tacttcaaag acccggtgtt tcaggcgcgg gcctcgatcc 50280
ccgcgccgaa ggccaccgac cggctgcgcc aggttgtcga ggcaggggag ggcatcacga 50340
tatgagccgc atcctgaaac gcatcacgaa aggcgtagcc gtcgccggcc tggccgccct 50400
ggtgctggcc gccggcagct atgccgccgg tgcccgcatc aacaccacca agagcattcc 50460
ggtcggcctg tactggacga gcagcgcgcc ggtggagcgg ggcgcttacg tgctttggtg 50520
cccgccgaag gtcggcgtgt tcgatgacgc caaggaaagg ggctacatcg gggccgggtt 50580
ctgcccaggc gactacggct acatgatgaa gcgcgtttta gccgctaaag acgatgccgt 50640
atcggtggcc gatgacggcg tgcgcgtcaa tggcgagctg ctgccacata gcaagcccat 50700
ccaggccgac aagtccggtc ggcccttgcc gcgcttccag gccagcacct acacgctggg 50760
caatgccgag ctgctgctta tgtcggacgt gagcgacacg tctttcgacg gccgctactt 50820
cggcccgatc aatcgttctc aagttcagac cgtgatccgt ccggtcatca cctggtaggg 50880
gaggaaacca ccatgcgact gttcatcgcg gaaaaaccat ccgtcgcaaa agccatcgcc 50940
ggcgagctgg gcatcaccgg caagggcgac ggcttcatcg agtgcggcgg cgacaaagtg 51000
acctggtgct ttgggcacat gctggagcag gccgacccgg acgagtacac gcccgatgac 51060
gtgccgaccg gcaaatcagg caagaagcta tggcgcgtcg atgagctgcc catcatcccg 51120
cagacgtggg ttcttcaccc gaaggacgat gccaagaagc agctcgccgt gatcggcaag 51180
ctgctgaaag aagccaagga gatcgtcaac gccggcgacc ccgaccgtga aggccagttg 51240
ctggtcgatg aagtgctgga gcacttcaag agcagcaagc cggcgcgccg cttttgggtg 51300
tctgcccagg attcggtttc cgtcaagcgc ggcctggccg ccttgaagga aaacaccacg 51360
tacaagggct gggccgatgc cgcacgcggg cggcagcggg ccgactggct gatcggcatg 51420
aacctaagtc gcgcctacac cctgcgggcg cagcgcggcg gttcgcgcgc cctgctgacc 51480
gtgggccgcg tgcagacccc gacgctcgcg cttgttgtcg gccgcgaccg cgagatcgag 51540
gcgttcaagc cggtgccgta ccacaccatc aaggccgtgg tcgagcacgc cggcggcagc 51600
ttcgccgccg cctggaaagc gaaggaagat caagccggcc tggacagcga aggccgcctt 51660
gttgataccg ccgtcgcgga tgccctggtg gccgccgtca agggccagcc gggcaccatc 51720
gccacctaca agcaggaagc caagaagaag aaccagccgc tggccttcgc gctgtccgac 51780
attaccgcgc tggcgtccgc gcgctacggg tacagcgccg aggacgtgct gaacacctgc 51840
caggcgctct acgagacgca caagctgacc agctacccgc gcaccgactg cgcctacctg 51900
ccggagtcgc agcacgccga cgcgccgcgc gtcctggagg ccgtcaagca cgtgaacccc 51960
gagctggccg gcctggtcga tgctgccgac ccgcgcatca agtccaagac gtgggacgac 52020
tcgaagatca ccgcccacca cggcatcgtg ccgaccatgc agaaaggcag caaggccggc 52080
ttgagcgagc gcgagcgcaa catctacgag ctgatcgtcc gcgcctacct ggcgcagttc 52140
tacccgctgc acgagtacat gcaaaccacg gtcggcgtgg agatcgccgg cgagaacttc 52200
gccgcgtccg gcaaggtggt cacgcgcaac ggctggcgtg acgtgttcga gcaggccgac 52260
gaagaggacg ccaaggaagg cgacgacgag aacggcgcgc agaccctgcc accgatgaag 52320
caaggcgacg gtgtgacctg caccgaggcc acccgacgcg atgcgaaaac caagccgccg 52380
gcgcgcttca ccgagggcac gctgattcgc gcgatggaga acatccacaa gttcgtgagc 52440
gacccggagc acaagaagat gctgcgcgaa ggcgatggca tcggcacgtc cgccacgcgc 52500
gcatccatca tttccgagct gaaacgccgc gagttcctgg aaaccaaggg caagcagatc 52560
gtcagcacga cgctgggccg cagcgtcatc gacgcgctgc ccgaggtggt gcgaagcccg 52620
gtgctgaccg ccctttacga acgcatgttg aagggcgtcg agctgggcac ggcggccctg 52680
gacgccttca ttacgaagca agaggcgttc atccgcgacc aggtggcgaa ggccaacagc 52740
ggagccgtga gcatcgccgg cggcaaggaa gccacgccgg tttcgtccct gcacaagtgc 52800
atggcttgcg gcaatggctt gtcgcgcagg ccggccaagc gcaaggggca attctggtgg 52860
ggatgcagca acttccccac gtgcaagcag acctatcccg acctgaaagg ccggcccgac 52920
tacagcaagg gccgcaatgg cccagccgag taaccaccaa gacgaaggag catgaccatg 52980
aaccagcaaa acaccaccca ggccgatgac gtgctgcccg agctgcccgc cattgacgac 53040
aacgcggccg ccgccggcgc gctgcgcgag aagctggccg acgcggcgac gcccggctac 53100
caggccgagt tcgaccccga ggaagccgag cgcgccggcg cgttcgtgga ggacgcattg 53160
agcgagcagg acgccgcaga gagcggcgac gacctggcgg ccctggcctc gcagggcgac 53220
gaccaggccc cggccttcat caccgaaggc ggcccgagcg acgaccttcc gcccttcgtc 53280
aatacgacca acatccgcga gctgtacgac tggaagccgg gcgaatcgct ggacgaggcc 53340
atcgcgcgca agaaggcgca ggagggctaa cccatgccga tcaaccaggc cgaggcgcaa 53400
gccgtcaccc tggcgttcgt ccgagactat cccggcgcgc tggagctggc ctatcggttc 53460
cgcgacaaca ccggcgagct gtacggccac cgggcagcag aagtcccggc agacctcaag 53520
ggcggctatg tcccgaagga gacgatccac aacggccggg cctaccgtgg ccgcgtggac 53580
gtgcccctgg cgaacatgga cgacgccgcc gacctggtgc tgacgctgcg gcatgaagtg 53640
ctgggccact acggggccaa caccttcaag ccggccgaga aacgcgccct gctggacggc 53700
ctgatcgccg cccgcgagga accgagcctg aaaccacgct gggaggacat caaccgccgc 53760
tatgccggcg cttcgatgga cgtgcgcgcg gaagaggttt gggcgctgca ttgcgaaagc 53820
atcgcgcccg gccagcacgc gggccaggcc caggtgctgg agcgcggcca gcagtcattc 53880
atggaaacct gcatcgcccg cgtgcggccc atgcaagtcg ccgacctgca caacatcgtg 53940
tgcatggtcg cggatggcct tcacgaccgt tccaggacgc agcagacctt tccgcagatc 54000
aacgaactat tccgaaggga tgaagcaatg gaacccaaga agccatttca tgaagtcgtt 54060
gccgaaaaac tgatcgagca gctcaaggca ggcaccgcgc cgtggcaacg gccgtgggag 54120
cctggcgagc cgaacgccta cctgccgatg aacccgacca ccggcaagcg gtacaagggc 54180
atcaacgcca ttcacttgat ggcccagggc cgcagcgatg gccgttggat gacctacaag 54240
caggccgccg ccgtgggcgc gcaggttcgc aagggcgaga agggaacgcc tgtccaatac 54300
tggaagttca gcgaggaaca gaacaaggtc gatgagagcg gccggcccgt tctcaacgcg 54360
aagggcgagc cggtcaagga aaccgtgcag ctcgaacgcc cgcgcgtgtt cttcgccacg 54420
gtgttcaacg ccgagcagat cgacggcctg ccgccgatcc agaagaagga acagacctgg 54480
agcgccgtcg agcgcgccga gcacatcctg aaagcgtccg gtgcgtcgat cacgcacgcg 54540
cctggtgatc gcgcgttcta ccggcccgcg accgacagca tccaccttcc cgaccggggc 54600
cagttcccca ccgccgacaa ctactacgcg accgccctgc atgagctggg ccactggacg 54660
ggccacccgt cccgccttga ccgcgacctg gcccatccct tcggttccga ggggtacgcc 54720
aaggaagagc tgcgcgccga gatcgcttcc atgatcgtcg gcgacgagct gggcatcggc 54780
cacgatccgg gccagcacgc cgcctatgtc ggttcctgga tcaaggccct gcaagacgag 54840
ccgctggaag tgttccgcgc cgctgccgac gccgagaaga ttcacgacta cgtgctggcc 54900
ttcgagcaga agcaggttca ggaacaagag caggcccagg tgcagcagcc cgagcagcac 54960
tactccgatt tgacgctcca ggccctggtc gaaaaacacg gctgggaggt tgcctacagc 55020
ggcttgcaag agcctggccg tgtggattcc gtccatcgca cctttgaagg tgtcgggccg 55080
ctgggcacca tgaccacccc gaacggcgag cggcgcttgt cggccggcta ccacgacgac 55140
gccgagcgcc ggcgctacgt cacgttgaag ctgggcgacc aggtgatcgg cgatgccgat 55200
ggccgcgacc agaagccgga agagatcgcc cgccagatca acgccaaggc cgagcagtac 55260
gccgacgagc ggcgcgtcaa gaacggcctg gagccgatct acagcatcgc ctcgcagcag 55320
gccgaggccc agcagctcaa tgatgagctg cgcgagcacg gccaggagat cgtttctcac 55380
ctggacgcgc aatcgcggtg ggcggatggc gagcgcatct ttgccttcca cgaacaagac 55440
gaacaaccgc accaggtcag aagcctggcc gagctggaga gctacgcgcc cgatcaactg 55500
ctggccctgc cggcgctggc ccaggagcaa gaggcaaagc ccttgtccgt ggccgaacgc 55560
acggccgagc agcacgaggc catgaaggct gccgacgatg ctttccagcg cgagctggtg 55620
cgcgcctatg gcgagaacaa cgccggcgat gcccgctaca agacccagca cgacgatgag 55680
gccgtgcaga aggccggcga tgccttcgtg gccgcgtcga acgcctggcg tgaggccgtg 55740
cgcgatgccc gtcaaaccgt tgcgagtcag gaggccacga tgcaagtacc ccagcagacc 55800
gaccaggccg aagcctggat gttgaagcac gtcgagctgg gcacggtggg ccgtgcgctc 55860
gaaggcgcga ccctggagca gatcgaccgc gccctggacg tgctggatcg catgcagccg 55920
atgaacacgc aaaacgagtt ctggacgcgg cacgagctgc cctacgacct ggagccgctg 55980
gaagcgaaga tcaacgacgc aatagatcac ctggtcgaag agcgccggcc ggatgccgta 56040
gtcgctgcaa cgcggcttga cctggcaacc ggcaacacca attcacgcga gcgcgaccgc 56100
caagccttcc acctggcggc cgacgatgcg ctgggcttcc ccctgcccca cgactggacg 56160
ggtgaagtgc gggttgtcgg cgtggtcgag cgtgacggcg agacgcgggc cgccgacctg 56220
gccacggaga cgccggaggc gtaccacctg tacgcccgga agggcgaagc gcagttcggc 56280
gaggatgcct ttgcctacct caccgccacc cgcacgctgg gcgaggccga cgagctggcc 56340
gaccgcctgg cgctgatcga cgccaattcg cagacggacg agtacgagaa ggcgacccgg 56400
cttgcccgcg tgcaagagga tcgcgtccgc cgcgacccga acagcaccga cgaggacatt 56460
tcagcggcta aagaagcccg gaaggcgctg gaggccaaag cgttcgtcgc ggccgaggaa 56520
gccaagaact cccagcgggt cgatgacgag cgcggcgact ccgtggagca ccaggcaccg 56580
caggaacagc gcgtggctgc ggccgaccag cagccggccc aggccagcca ggcaaccgaa 56640
cggcagtaca tcagcgtgcc gttcaaagag aaggacgagg ccaagcagct cggagcgcgc 56700
tgggatcgca aggaacagtc ctggtacgtg ccggcgggag tggatgcggc ccccttcgcc 56760
aaatggtcgc agggagccgc tacgccggcc gtagaggcca agccgaccca gcaggcaccc 56820
gaggcccagg acggcggcca gaaggccgca caggccgtcc agcaggcccg gcagtacctt 56880
gccgtgccct acgagcagcg caacgccgcc aaggccgccg gcgcgctttg ggacaaggcc 56940
gcgaagtcct ggtatgccgg ccctcgcgcc gacatggcga agctggaaca gtggaagccc 57000
gagaacgtgc aggcccagca ggggccagcg atgacgccaa gggaagagtt cgccgaggcc 57060
atgcgcagcg ccggcctgtt caccggcagc aacgcccagg gcgatcaccc gatcatggac 57120
ggcaagcggc atcgcgtgcc ggtcgagggc ggcaagaagg gcacgctcga tggcttctat 57180
gtcggccacc tggacggcca cccggccggc cggatcatca acaacaagac cggcacggac 57240
attacctgga agagcaaggg ctacgccttg agcgaccagg agaaggccaa gctccaggcc 57300
gaggcggccg agaagctggc ccagcgtgcc gccgagcaag acaagctcca ggaagccacc 57360
gcgcagcgcg tcggccgcca gatgcccgac ctggtgccgg tcgagcagcc gacgccctac 57420
ctgcaagcca agggcatcga ggcccacgcc ggcgtgttca ccgaccgcga gggccagaaa 57480
acctacatcc ccgccttcga cgtggacggc aagcagtgga cgatgcagta catccaggag 57540
gacggcacca agcggttcgc caaggacagc aagaaggaag gatgcttcca cccggtcggc 57600
ggcatggatg cgctggccgc agcgccggcg ctggtgatcg ccgagggcta tgccacggcc 57660
gcgagcctgg ccgaagggct ggggcatgcc accgtggccg cgttcgactc gggcaacctg 57720
ccgcacgtgg cccgcgccct gcgcgagaag ttccccgaca agcccatcgt gatcgccggc 57780
gacgacgaca aggcccagga gatcgagcgc ggccacaacc ccggccgcgc caaggccgag 57840
gaagccgcga aggccgtagg cggcaaggcc atcttcccga tcttcgcgcc gggcgagcag 57900
caggcgaacc ccaagggctt caccgacttc aacgacctgg cgaacaagag cgagctgggc 57960
cgcgacggcc tcaagcgcca ggtcggcgca gccgtgggcc aggtgctgat cgaggaaggc 58020
cgccgacagc agcaggagca gcgccaggag cgtgcggaga agcaacagca gcagccggaa 58080
cgcccgcgcc gcgcggcacg catcggctaa agtcccggag ggctgcaacc acacgcgcgg 58140
tttcctccct ccctggccgc cggcagtcat cgccgcctcg caccccgagg cggctttttt 58200
ttgctcgagc atcgccgcat ccgacgatgc caccaggccc gcgcacgtcg agctgcagca 58260
tcgccatttc cggcgatgca cgccggcgcg gtcgaccatc gccagttccg acgatggcgg 58320
ccgccctgcc cgcctggatc tcgcatcgcc atttcggggt ctcctcgttt tcagtgcaat 58380
aagtgacggt acgcaaagct agcactggcg cgggggtggt ctgggtagac cgttgatttc 58440
attgactttc ctgttcgctt tgtaaacggg tatggtggcc tcccactttt gaggttcacg 58500
atgcagggtt ggcacacaac gtttttgggg atgcgtgggc tcccccgcga tatcagcgac 58560
ttcgagatga aggcattttt caccttcgat ggtgccgagc gcgacgcaat caatgcacgc 58620
cgaggtgatt cccacaagct tggtctggcg ctccatattg gtttcctgcg catgagtggg 58680
cgtttgctcg gtgcctttcg ggtaattcca gtagccttgt ggcgccacct tggcaacgag 58740
cttggcattg cagcaccaga agtcgcctcg ctgagagcca tgtatgaacg cgggcgcacg 58800
ctattcgatc accaacaagt agcctgcacg gtccttggat tccagtggat gagcgagcac 58860
cagcgccgct cactggtacg tgaactgcgc gacgaagtgg cgcgctgcgc cgaccgcgat 58920
cagctactcg tgcgggcgcg tcaatggctg tacaagaaca agctggtgat cgtgcacgag 58980
cgggcaattc ggacactgat tgcggcggca cttgcccagc ttgaagttga aacaggcacc 59040
gccatcgccg ccagcgttga tccagcaaca cttgatcgct ggcgagcctc agtttcagag 59100
ctgcgcccag atggacaaac ccagcagagt tggctatggg ctgcaccggc gaaacactca 59160
acccgccaaa tcagcgaggt actggagcgc atcgacctgc tttacacgct ggacgttcat 59220
aagcacctgg cagacatccc cgatctcatc ttgcgccgct acgcgcgccg acttgtctcc 59280
aggccgccct cagccggagc caagatcaaa gagccagcgc gcaccgtgga ggtcgcatgc 59340
tttcttcggt attgcctgtt caccaccaca gaccagttga tccttatggt gcagcgccgg 59400
atcgccgatc tgtggcgtca ggctgccgcc gatgtccccg ctaccgtcaa ttgggccgca 59460
atgtacaaaa cgctgctcgg cgaacttgtt gccttgagcg cgcaaggtgc ggtgccagat 59520
gctgagttgc gtgcccgtct tgaagccttg atcaccgaaa cccagaaacg caaaccaccg 59580
agcagggcct ccctggtccg cgagggattg attgatggaa ttcgccccgt gcggtcgttg 59640
ctcgtcgcca ttgcaaagct gccctggcag gccaccggcg agcatcctgc catcgagtac 59700
cttgccaagc tgcaagcttt atatctcaaa ggatccagaa agctgccagt tgaagtggtg 59760
gcaccaagtc tgggaatgat ctggcaggtt tcgatctcca gcccagaccg ggaacgggcg 59820
tttcaggcgt tggaggtggc caccctgttt gccctgcgcc gcgcggtgcg caatggctcg 59880
gtctggattg agcacagcct gagctttcgg ggtcgtgcgc gcttgttctt cacggacgag 59940
cgttggcagg cagagtccaa gaaacactat gcccgtctat cgttacccag caaggctgcc 60000
actttcttga agcctttgct ggccagagta actgccggtg tcgatgcggt ggccgctgca 60060
gcccgcagtg gcgtactgcg cgtggatgat gaactccatt tgtcgccatt gcccgcagag 60120
gacgaagacc cagaagtgac caagctgcgc gcggctttgg atcaccgcat cggtgaggtt 60180
caattgccgg aagtgattct ggccgttgac gcccaggtgc gctttagctg gatcatgctc 60240
ggacgtgagc cgcgctctac cgacgagctg ctgatggtct atgccggcat catggcccac 60300
ggcaccagtc tgactgcggt cgaatgcgcg cgcatgattc cgcaattgtc tgccaccagc 60360
attcgccagg ccatgcgctg ggcgcgggac gaacggcgtc tgagccaggc ctgccaggct 60420
gtgctggaat tcatgcagcg acacccgatt gccgccacct gggggcggtc cgatttggca 60480
tcttctgaca tgatgaccat ggagaccacc aaacgggtgt ggcaagcccg gcttgatcct 60540
cggcgcaaca caccttccat tggaatctac tcccatgtaa aagaccggtg gggcatcttc 60600
catgcgcagc cctttgtgct caatgagcgc caggcgggcg tggccattga aggtgtcatc 60660
cgccaagaaa agctggagac cagccagctt gctgtggata cccatggcta caccgacttt 60720
gccatgtcac atgcccgttt gcttggtttt gatctttgcc cgcggttgaa ggaactcaaa 60780
cagcgccacc tctttgtgcc acgcggcacc aaagtgcccg cagaaatcgc tgcggtgtgc 60840
gaagccaatg tcgacgtcgc tttgatcgaa aagcattggg atagtctggt gcacctggca 60900
gcctcggtca tgagcggaca tgccagtgcg gtggcagctc ttgcgcggtt cggttctgcc 60960
gcccagggcg atccaatcta tgaggctggc gtgcaattgg ggcggttgct gcgtacggcg 61020
tttttggctg actactttgt caaggacgct ttcaggaacg agttgcgccg ggtgctcaat 61080
cggggcgagg ctgttaacgc cctcaagcgc gccatttata ccggccggat cagcccggcg 61140
caggccaaac gtgtcgatga aatgcaggct gtggccgatg cgttgagcct gatggccaac 61200
atcgtgatgg cgtggaatac ctcacagatg caggcggtcc tggatcgctg gtcgaaccgc 61260
cgccaggtca ttccaccgga actgatcggg aagattgcgc ccaccaggct ggagagcatc 61320
aacttgcggg gtgtgtttcg cttcccggtt gaccgctatg ctgaccaaat cctgccttcg 61380
cggccaaatg catcgataac tggcaccaat ggatgaaacc gaccacggtt tgacgccacg 61440
aatcgcagat ttgaaagtga acaggaaagt caatgaaatc aacgatctac caacaccacc 61500
tccgcgccag tgctagcttt tcgtaccgtc acttattgca ctgaaaacga ggagacccca 61560
tggcacacga acgctgaccg tcggactcag ctgggacctg ccctaccgat ggcagctggg 61620
accgggcgag ctgagcagct tcctggaggc ttcctacgcg tactggcaga tccagtcggc 61680
cgaccgtgcc gggctttcgc acctctcgca gctggcgctg gttccggtgc ttcgctaccg 61740
ccctgcggat ggcgcttcgc cctggttctt cgaaacgggg ttgggggtga ccgccacctc 61800
ttcggtctac aagacccggc agaagagctt ttcgacgagc ttcaacttca gcacccatct 61860
ggccgtgggt cgaagcttcg gcgagcagcg ccggcacgag atcgcgctgc gtctcgaaca 61920
cttttcgaac gcaggcatca agcatccgaa cccgggtgag aacttcgtcc agatccgata 61980
tgcccgccgg ttctagcgcc gttgcgcaag gtagcgtgcc ggtggctggc ccatggcctt 62040
cctgaacatc gtgatgaagg cactcaccga gtcgtagccc agctcatcgg ccacccgttg 62100
caccgagagg cccgagttca gcctttgcag cgccacgatc aggcgcaggc gctggctcca 62160
acgtccgaag gacatgcccg tttcgagtcg aacgaggcgg gtcagggtcc gctcgctcat 62220
ggccaggcgc gacgcccatt gcgctgccgt agtccggtct gccggatcgc gcatcagcgc 62280
atcgatcagc cggcgcagac gcggctcctc cgacaccggc aagtgcatgg cctcggttgg 62340
catgcgcacc agttggtcga gcagcacggc gtgcagccgt tgcgcctgcg ggtcgcgctg 62400
cgcgccctct ggcaaagcgg ccacgtgaag aatgagttcg cgcagcaagg ggctgatgct 62460
caatgagcag caggtctgcg gcatgacggt ggcggcgtct tgcacaaaaa gaaaacaggc 62520
acttccgtcc gccgaaacgc tcaggctgtg cggcacgccc ccgggaatcc agaagccacc 62580
gtgcaacggc gccatccaca gcgcaccggg cacttcacag gtcacggtgc cgcgcagcag 62640
catcgccaac tgaccttgcg gatgccggtg cgtcggcaat tcctcggcca gcgcccgggt 62700
gcggatccgc acggcggcca cgcgggcatc catcttttcg aagtcgaaag tccaaggggc 62760
tcggctgcgt gtgcctcgcg gttcatcttg accggatttg ctcatattta gtccgcccgc 62820
aggcattcga acccgcgctt cgtcactata ttcttctgtg gcatcccgca tcacttcgga 62880
cctccatgca ttcctcacaa ccggcagatt cttcgccccg cgccgcggtt ctcatcgctg 62940
gcatcctgct catcgcggcc agtctcaggg caccgatcac cgcgctcggg cccttgctcg 63000
aacccgtgcg cgagaacttc gcgctcaacg cttcgcaggc cggcctgctg acgacgctgc 63060
cacttctcgc gttcgccttg gtgtctccgc ttgcagcacc cattgcacac cgattcggtc 63120
tggagcgcgt gctctttgca tcgctggtcc tgctcgtcgc tggcatcgcc gtgcgctcgg 63180
gcggcacagc ttcggcgctc tatgcgggaa cctgcgtgat cggcggcgcg attgccattg 63240
ccaacgtctt gttgccgagc ttgctgaaaa gggactttcc tcatcatttg gcgaagctca 63300
cggccgcgta tgcactcgcg atgatctctg ccgcgggcct cgcttcggcc gtggcggtgc 63360
cgctggacca cgcgcttggc gccggatggc cggcctcgct gggcgccgtc gccgtgctgc 63420
cgctcgctgc cgcattgctg tggctgccgc aactgcgctt tcgaaccgtc tcggcaccgg 63480
gccttgccac gggtgtgcac ggagcctcgg tgggggcctc ggtgtggaag gcctctctgg 63540
cctggcaggt ggccgggtac ctgggcctca cctgcttcat ctactttgcg gcgattgcat 63600
ggctgccttc gattctccag gaggccggct tctcgagcgc ccgcgccggc acactgcatg 63660
ggtggatgca gcttgcaggg gccgtgcccg cgctgctgct gatgccgctc ctgcaccgca 63720
tgaccgatca acgctggatt gccttcgctt cgccggcgct gagtgcctgt ggcctggccg 63780
gcctgctggc cctgcccggg ttgacaccgc tgtggctctt tgccttcggc atggggatgg 63840
ggtcggcttt gatcctgagc ctggccttcg tcgggctgcg tgccgggagc caacagatcg 63900
ccgcatcgct gtccggcatg tcgcaatgca tcggttacct gctggcagct gtcggtccca 63960
cgctggtggg ctacctgcac gaggccgcag gcaactggac gatgccgctg gcggcctgcc 64020
tgctcctgtg cctggcgatg tgcgcgcttg ggctgtcggt gggccgcgcc gtgcacatcg 64080
gcaaatgaac ggtgcagatg tccgagcggc tcgaggcatg cgtgctttat gtatcagatt 64140
cacgcggatg gtaggcgcgg ccagcgggcg gcggtaggtg ccatggcgtg accacggtgt 64200
tgccttgtcc acaccaatgc accaatgcgt ctatgcaccg gcaccgcgat tggtgcgccg 64260
gtgacaacac ggtgcacccc gacacatgcc ttccgccgcc gcgcacgcct gcctagcatt 64320
gaatcgttcg caagcctctc ctgcacaacc cgatgccggc cgtcccgcca aagcgaacgg 64380
cccgcctcaa cccaacggat atcccatgaa gctctatcac atgcccggcg catgctccct 64440
cgccgacctg atcgtcctcc agtggatcgg cgcagaccac gaaccggtgc gcatgagcct 64500
ggaaagcatc aagtcgcccg actaccttgc catcaatgcg ggtggcaccg tgccgctgct 64560
ggctcacggc gacctgtcgc ttaccgagaa cgtcgccatc ctcggctacc tggccgacct 64620
gtaccccgaa gggcgcctgc tgggcgacgg gtcgccgcgc gcccgcgccg aagtcatgcg 64680
ctggctcggc tttctcaact cggatgtaca caaggcgttc aagccgatct tcgtgccgca 64740
gcgtttcctg aacgacgcgg cgatggcgcc aatcctcgcg gacaacgcgc gcggccatgt 64800
gcgcgagtac ctcgcgcggc tggacgatca actcgacgga agggactggc tgaccggcga 64860
acgctccatc gcggacccgt acctcttcgt gctctcgcgc tggtcggcag ccaggaaggt 64920
cgacatgcaa ggcctggaca acctcgcgcg attcgtacgg atgatggacg ccgacgccgg 64980
cgtgcaagcc gcgctgcgtg ccgagagcgg catgccgcag taaccccggg gcacgccatg 65040
agactgtcgt tgacctctgc tctcaaagcc ctcgaacccg tctgcgccac gtcgactgca 65100
ctggcccgct ggtacgccga gcagcccgcc gttcggcggc tgtgggccat ccaggcgacg 65160
gacgggcccg cgctgcgggt catcgtgatg cttgaaccct cgctcgacgg cgatgaaatc 65220
agtcccgcct ggatggcgca cggcagccga tgggcgcgcg acctgcgcga acggctgacc 65280
ggtaccgtgc aactggaacg catcgacggg ccgctgcccg aagagttcga gatcgacggc 65340
gaaggcaccg tgctctccgc gctgtgctgg cgcgactcga ccgcgcccca agcgtgagcg 65400
caccggcgat cgtcttcccc caactccctt aaccatcaca tgagcaaagc cctcgttcag 65460
gcgtacggcg caccgagtcc cgccacgacc actcgccaga gcggggtcgc tggtgtcgag 65520
accgtcatcc tgccgccgac gcgcgacctc ggagacggct tcaaggttcg aagggcgttg 65580
ccgtctgcgc gccggcagat ggtcgggccg ttcgtctttt tcgaccacat ggggcccgcg 65640
accttcacgg cgggccaagc tttcgacgtg aggccgcatc cgcacatcgg cctggccacg 65700
gttacttacc tgcgtattcc acgccatcgc gggcaccgtt ccacgctgat ggcgggcagc 65760
gttccacgtg atcgcgggca gcagtccaga cgatcacggg cagcgttcca tttgatcgcg 65820
ggcacgattc cattctgatg gcgggcagcc gccgcggccc cggggtgacc tatcgcaacc 65880
gggcatagcc tcgcgcgctt tgtgttgaac gaagcgagag cgaggcgatg cccacaccga 65940
aggtacccat gagcaagatc agaagagttc tccaactgct gcacgaggcc gggttgagcc 66000
agcgcgaggc ggcccgcgcc gctggcgtag gcaagacctg tgtgagcgac atcgccagcc 66060
gcgcgcgggc cgccgggctg tcctgggcgc aggcccgcga gttggacgac gatgccctgc 66120
aggcgcggct gcaccccgcg cccgtggccc gcagcgcccg ccacctggag cccgattggt 66180
ccgagctgca ccagcagctc aagcgccccg gcgtgacgct gcagctgctg tgggaggagt 66240
accagctcgc ccacgacggt cgggcgtaca agtacagcgc cttctgcgag aagtacgcgc 66300
gctttgcacg aggcctcaag cgctccatgc gccaggtgca caaggccggc gagcggctgt 66360
tcgtggacta cgctggccag acggtggagc tggtggacat gttcagcggg gagatcacgc 66420
gcgcgcagat cttcgtggcc gtgctgggcg cctccaacct cacctatgcc tgcgccacgc 66480
gcacccagca ggccgaggac tgggtgcgct cgatcatcgc ggccctgcag ttcatcggcg 66540
gcgtgccgcg cctgctggtg cctgaccagc cgcgcgcgct gatggcccgg cccgaccgct 66600
acgagcccac ggcgcaccgg ctgctggccg agctgggcga acactacggc gtggcgatcc 66660
tgccggcgcg tcctgcacac cccaaggaca aggccaaggt ggaagtcggc gtgcaggtcg 66720
tggagcgctg gatcctggcg cggctgcgcg gtcggcgctt catcaacctg gcggaactga 66780
accacgccat cgcgccgctg ctcacgcaat tgaacgagcg gcccttcaag aagctgcccg 66840
gctgccggcg cagcgccttc gaggccatgg agcgcgccgc cctggcgccg ttgccggcca 66900
tgcccatgca ggtggcccgc ttcaagctgg cgcgcgtgaa catcgactac cacgtggagt 66960
acgaggggca ctactacagc gtgccgcacc gcctggttgg ccagcaggtg gagctgcgca 67020
tcaccgacaa gacgctggag gcgctgcaca agacccagcg cgtggccgcc catgtgctca 67080
atggccggcg cggtggccac agcaccacgg ccgagcacat gcccgcctcg caccgcgcgc 67140
acgcgcagtg gagcccggcc aagttgctcg gctggggcca gggcatcggc gaggcctgcg 67200
cggccgtggt gcgctggcag ctggagcacc gcccgcatcc cgagcagggc taccgcgcct 67260
gccttggcct gatgcgcctg gggcgcgagt acgggtccga acggctggag gcggcctgcg 67320
cccgggcact gtccatccgc gcaccgcact acaagagcat cagctccatc ctggcctcgg 67380
gcctggaccg tcatggcacg tcccaacacg acctgccggc catgccggtg cacgagaacg 67440
tgcgcggcgc gggctactac cactgacccc atgccgaccg aaggaacaca ccccatgctc 67500
gctcaaaaca ccctggatca actgcgcggc ctgcgcctgg acggcctggt ggccgcactg 67560
caggatccgg ccaccacgca cgcggcggcc gatctggact tccacgcccg gctggcgctg 67620
ctggtgcagc gcgaggtcga ctggcgcgac gacaagcggc tctgcaggct gctcaaggcc 67680
gcgcagctga aggtctcggg cgcctgcatg caggacatcg actggcgcgc ctcgcgtggg 67740
ctgaaccgtg cgctgatggc cgagctggcc acggcccagt gggtgcgcga tgggcgcagc 67800
gtgctgctga ccggggccac tggctgcgga aagacctggc tggcttgcgc gctggcgcgc 67860
caggccgcgc gcgccggcca cgccgtgctg tacacccgca ccgcgcggct gctgcaggag 67920
ctgcacgtgg cccatggcga cggcagcttc aagcgccggc tcggccaact ggcgcgcgtg 67980
caagtgctgg tgctggacga cttcgcgctc acgcccattg cggcccacga gcgcggcgac 68040
ctgctggagt tgctggacga ccgcgtgggc agccgggcga ccatcatcac cagccagttg 68100
ccgccggcgg cctggcacgc atggttgggc gaacccacgc tggccgatgc gatcctggac 68160
cgcgtggtgc acggggcgca caagatcgcg ctgaagggcg agtcgatgcg acgcagtcgc 68220
gccaacgcca gcgctaccac cgaggccgcc atggccgagg acaccagcca gggcggctga 68280
tacctgcgct gtcgcgcagc aggccgtgcc atgccagcca tgcgcgcggc ctgcaccgcc 68340
ttgcccaccg cggcgctcgg ttcgtcgcag gcgccgaccg ccgccgtgga caaggctgcc 68400
aagcgatcca tcaaagcgtg ttcatcgcct tcggcgcacc ggttacccac ccggcgctgc 68460
gcgccgcgtg ggcaaccggc caaagccgcg ttccacgctg atcgcgggca cctgcactac 68520
catcgccgcc gtcactccgg gccgcgcgcg gctgcccgcc atcgcgtgga acgctgcccg 68580
cgatcagcgt ggactgctgc ccgccatcgt ctggaatggg tgcccgcgat cagtggaatg 68640
cgcacttacc tgatcgctgg cgagatcatg catcgcgacg gcctgggcaa cgtgcaggcg 68700
atccgtcctg gcgaggtcaa ctggatgacc gccggttcgg gcatcgtgca ctccgagcga 68760
acgcccgaag ccgagcgccg gcccggcgcg tcgctgttcg gcattcaggc ctgggtggcg 68820
ctgccgaagg cgcacgaaga ggccgaaccg gccttcttcc atcacgccgc ggcggccatt 68880
cccaagaccg aatcggacgg cgctgcactg acgctgatcg cggggcgaag cgatggactg 68940
gtgtcgccag tgcgcacgta ctcggacatg gtctacgccg acatcgtgct cgaagacgcc 69000
gcgcgctacc aggtgaaggc ggagcacgtc gaacgtgcgg tctacgtggt gtcgggagcg 69060
ctcgaggtac ttggtcaggc cggccgcttc gaagccggcg aactcgtggt cttcaagccc 69120
ggcgccgaac tcgtgctgcg cggggccggg gcgacccgcc tgatgctgat cggtggcgaa 69180
ccgcttgccg agccacggca tatctagggg tctcctcgtt ttcagtgcaa taagtgacgg 69240
tacgcaaagc tagcactggc gcgggggtgg tctgggtaga ccgttgattt cattgacttt 69300
cctgttcgct ttgtaaacgg gtatggtggc ctcccacttt tgaggttcac gatgcagggt 69360
tggcacacaa cgtttttggg gatgcgtggg ctcccccgcg atatcagcga cttcgagatg 69420
aaggcatttt tcaccttcga tggtgccgag cgcgacgcaa tcaatgcacg ccgaggtgat 69480
tcccacaagc ttggtctggc gctccatatt ggtttcctgc gcatgagtgg gcgtttgctc 69540
ggtgcctttc gggtaattcc agtagccttg tggcgccacc ttggcaacga gcttggcatt 69600
gcagcaccag aagtcgcctc gctgagagcc atgtatgaac gcgggcgcac gctattcgat 69660
caccaacaag tagcctgcac ggtccttgga ttccagtgga tgagcgagca ccagcgccgc 69720
tcactggtac gtgaactgcg cgacgaagtg gcgcgctgcg ccgaccgcga tcagctactc 69780
gtgcgggcgc gtcaatggct gtacaagaac aagctggtga tcgtgcacga gcgggcaatt 69840
cggacactga ttgcggcggc acttgcccag cttgaagttg aaacaggcac cgccatcgcc 69900
gccagcgttg atccagcaac acttgatcgc tggcgagcct cagtttcaga gctgcgccca 69960
gatggacaaa cccagcagag ttggctatgg gctgcaccgg cgaaacactc aacccgccaa 70020
atcagcgagg tactggagcg catcgacctg ctttacacgc tggacgttca taagcacctg 70080
gcagacatcc ccgatctcat cttgcgccgc tacgcgcgcc gacttgtctc caggccgccc 70140
tcagccggag ccaagatcaa agagccagcg cgcaccgtgg aggtcgcatg ctttcttcgg 70200
tattgcctgt tcaccaccac agaccagttg atccttatgg tgcagcgccg gatcgccgat 70260
ctgtggcgtc aggctgccgc cgatgtcccc gctaccgtca attgggccgc aatgtacaaa 70320
acgctgctcg gcgaacttgt tgccttgagc gcgcaaggtg cggtgccaga tgctgagttg 70380
cgtgcccgtc ttgaagcctt gatcaccgaa acccagaaac gcaaaccacc gagcagggcc 70440
tccctggtcc gcgagggatt gattgatgga attcgccccg tgcggtcgtt gctcgtcgcc 70500
attgcaaagc tgccctggca ggccaccggc gagcatcctg ccatcgagta ccttgccaag 70560
ctgcaagctt tatatctcaa aggatccaga aagctgccag ttgaagtggt ggcaccaagt 70620
ctgggaatga tctggcaggt ttcaatctcc agcccagacc gggaacgggc gtttcaggcg 70680
ttggaggtgg ccaccctgtt tgccctgcgc cgcgcggtgc gcaatggctc ggtctggatt 70740
gagcacagcc tgagctttcg gggtcgtgcg cgcttgttct tcacggacga gcgttggcag 70800
gcagagtcca agaaacacta tgcccgtcta tcgttaccca gcaaggctgc cactttcttg 70860
aagcctttgc tggccagagt aactgccggt gtcgatgcgg tggccgctgc agcccgcagt 70920
ggcgtactgc gcgtggatga tgaactccat ttgtcgccat tgcccgcaga ggacgaagac 70980
ccagaagtga ccaagctgcg cgcggctttg gatcaccgca tcggtgaggt tcaattgccg 71040
gaagtgattc tggccgttga cgcccaggtg cgctttagct ggatcatgct cggacgtgag 71100
ccgcgctcta ccgacgagct gctgatggtc tatgccggca tcatggccca cggcaccagt 71160
ctgactgcgg tcgaatgcgc gcgcatgatt ccgcaattgt ctgccaccag cattcgccag 71220
gccatgcgct gggcgcggga cgaacggcgt ctgagccagg cctgccaggc tgtgctggaa 71280
ttcatgcagc gacacccgat tgccgccacc tgggggcggt ccgatttggc atcttctgac 71340
atgatgacca tggagaccac caaacgggtg tggcaagccc ggcttgatcc tcggcgcaac 71400
acaccttcca ttggaatcta ctcccatgta aaagaccggt ggggcatctt ccatgcgcag 71460
ccctttgtgc tcaatgagcg ccaggcgggc gtggccattg aaggtgtcat ccgccaagaa 71520
aagctggaga ccagccagct tgctgtggat acccatggct acaccgactt tgccatgtca 71580
catgcccgtt tgcttggttt tgatctttgc ccgcggttga aggaactcaa acagcgccac 71640
ctctttgtgc cacgcggcac caaagtgccc gcagaaatcg ctgcggtgtg cgaagccaat 71700
gtcgacgtcg ctttgatcga aaagcattgg gatagtctgg tgcacctggc agcctcggtc 71760
atgagcggac atgccagtgc ggtggcagct cttgcgcggt tcggttctgc cgcccagggc 71820
gatccaatct atgaggctgg cgtgcaattg gggcggttgc tgcgtacggc gtttttggct 71880
gactactttg tcaaggacgc tttcaggaac gagttgcgcc gggtgctcaa tcggggcgag 71940
gctgttaacg ccctcaagcg cgccatttat accggccgga tcagcccggc gcaggccaaa 72000
cgtgtcgatg aaatgcaggc tgtggccgat gcgttgagcc tgatggccaa catcgtgatg 72060
gcgtggaata cctcacagat gcaggcggtc ctggatcgct ggtcgaaccg ccgccaggtc 72120
attccaccgg aactgatcgg gaagattgcg cccaccaggg gtcgtctcag aaaacggaga 72180
ataaagcacg ttaagccgat tgcagaggtc gcaacggcct gaacttgccc gcgccgatct 72240
tggcgctgct gcgccagagg taatcgccgg tcagattgat gtgttcccag cccaacggcg 72300
acaggtactg caatagcgtt tcatcgacgg catggccgtt gctacgcaac gcatgcgctg 72360
cgcgctccag gtagaccgtg ttccacagca cgacagccgc cgtcaccaga ttgaggccgc 72420
tggcccggta gcgctgctgc tcaaagctgc tgtcgcggat ttcgcccagc cggttgaaga 72480
acacggcgcg ggccagtgca ttgcgtgctt cgcctttatt cagcccggca tggacgcggc 72540
ggcgcagctc aacgctttgc aaccagccca ggatgaacaa cgtccgctcg atgcgcccca 72600
gctcacgcag ggcgacagcc aggccgtttt ggcgcggata gctgccgagt ttcctaagca 72660
tcagcgaggc cgtcaccgtg ccttgcttga tcgaggtggc caggcgcaga atttcgtccc 72720
aatgggcgcg gacgtgcttg atgttgagag tgccgccgat catcggtttc agcgcgtcat 72780
aggcggcttc gcctttcggg gtgtagagct tggtgtcgcc caggtctcgg atgcgcggtg 72840
cgaagcggaa gcccagcagg tgcatcaagg caaagacgtg atcggtgaag cccgccgtgt 72900
ccgtgtaatg ttcctcgatg cgcaggtcgg actcgtggta cagcaggccg tcaagcacgt 72960
aggtggaatc gcgcacgccg acattgacca ccttgttgtg gaacggcgcg tactggtcag 73020
agatgtgggt gtagaacgtc ctgcctgggc tgctgccact ttgacctcaa aaagcatttc 73080
ggcatggacc gtcggcacgt gagccatgaa gacctccagg ccgtcatcgc ggaggatgcg 73140
atcgacgtgc ggcctggcga catagtggtg ttccgcacgg gcttcacgga agcggttatg 73200
gcgatgaaca ggcagcccga caaggaaatt ctcgacgcca cgggcagtgt cctcgatggg 73260
cgggacacac ggctgctgaa ttggataacc gacagcggca tcgcggcact gtgcgccgac 73320
aactacgcgg tcgagggcct gcccgcgcgc gaggcgcttg gccggcggcc ctcgcttccc 73380
cttcaccagc attgcctgtt caagctgggc gtgccgctgg ccgaactgtg gtgggtgaag 73440
gatctggccg actacctcgg gcaggagggc cgcaccgcgt tcctgctgac cgcgcccccc 73500
ctgcggctgc ccggcgccgt cggctcgccc accacaccga tcgcgaccac gtgatgccga 73560
cgcccgatcg aactccgccc gtgcgcctcg ggcaaggccg ccattggcag gacatccaag 73620
tgggcgatcg attcgacacc ttcagtcgta ccgtgaccga ggcggacctc gttaatttcg 73680
tgtcggtcac gggcatgctg gaggccatct tcatcgaagc caactacccg gacggggcca 73740
ttgccggccg gccggtaccg gccgcgctga cctacaccct gatcgagggg ttcattctgc 73800
agaccatgat ccaggggacc gggctcgcca tgctcgagtt gacgcagaag atcctcgcgc 73860
ccgtgctggt gggcgacacg atccgggccg agatcgaaat catgtcgatc cagcccaaga 73920
agaccggccg cgccgtagtc gcatcggcga tcaccgtcct gaaccagcgc ggggtaacgg 73980
tcatgacgta cacagcggta cggttgctgg ccggccgccc gccccattcg aacccaaccg 74040
gaaaacaagg agatacccca tgaaatttcg tgccagcggc gtcattccag cggcgctgat 74100
gcctttcact caagacatgg agttggacct gaccacgttc aggtctcact tgcgtgatct 74160
cgcctccgtc aagggagtca cgggaatctg cgtcaacgga cacgcgtcgg aagtcatgtc 74220
ttgcacggag caggaacagg cccggatctt aacggtggct ctggaagaag taggcaacgc 74280
gctgccggtg atcggcggcg tctattcgga aagcagcatc gtcgcagccc agcatgccaa 74340
gcaatggcag cagctcggcg ccaacgcgct cttggtggtg ccaccggctg ctttcggcaa 74400
gggtgcccag cttcggcctg agatggtcat ggagcattac cagcggatcg ccgatgcatg 74460
cgacctgcca ctgatcatct ttcaatatgc gggcgcgcaa atgatggcga tccagacgct 74520
caccaatctg gctgacacca tccccagcat caaggcgatc aaagactact gcggtgaccc 74580
cgttgcgcac gaagagacgg tcagacagct ccagaatagc cacaggaagg tgaacgtgct 74640
gaccgcccac agctcgtggc ttctgcaatc acttgcgctc gggtgcgaag gcatcttgtc 74700
gggtgcaggc agcactgtcg ccgcgcttca ggccgagctg ttcgaggcga tgaaagacag 74760
cgacctgcca cgtgctcggg aaattaatga gcggctggac attccgaaca aggccttcta 74820
ccacgccccg atggccgacc agcacaaccg gatgaaagag gctcaagtcc tcatgggacg 74880
ctttccccat gccgtggtga ggccgccgtt gatgaagcta tcgacagcgg agatcgacgg 74940
catccgggcg gccttggcca aggccgtcat gttgaatcac taaggagacc agtatgacca 75000
tggaattcgc ttccggactc gtaaagcgct cggcatgcct gtcttgtaaa ccgctcaatg 75060
atcgacatca tgcgcaagct ctgattcctt tcttttccga gatcaactca cgttagtcag 75120
tgcctgttca taaaccgaga ggaacgggcc cctaatgact gctaccgacg agttgtacta 75180
cctgccggtg accgagctgt ctgagctcat cgccgagcga cgactggctc cgtccgaact 75240
gatgtcggcc gtgattgcgc gagccgagga ggtgaatccg aagctgaacg cattggtagc 75300
ccagcgcttc gaggcggcta cccgcgaggc agccgctgcc gacaacgagc catccagagg 75360
agtgctgcac ggtataccca tcacgctgaa ggatctggcc tatgagacgc cggatctgcc 75420
gtcgacctat ggctcgcgcg ccttcgcggg ctacgagccg ggcttcgaga cagtggtcgg 75480
acagcggttg cgcgcggctg gaacgatcgc gatcggccgg acgaactccc cggagttcgg 75540
gctcaccaac acctgcgaga gcgcgcagtt cggcccgacc tcgaatccgt ggcggccgga 75600
gcacacaccg ggcggatcca gcggtggcgc cggagctgcg gtggccgctg ggatcgcgcc 75660
gctggctgcc gccaacgacg gcggcgggtc gtgtcgcgtc ccggcgtcca gctgcggtgt 75720
ggtcggtctg aagccgagcc ggggccgcgt cccgtgggcg cccacctcgt acgagtattg 75780
ggccggcttc gccaccaatg gcccgatcgc gcggaccgtt gaggacgtcg cgctgttgct 75840
cgatgcgatg tcgggccccg tcgtcggcga gccgtacggc ttacccgcgc cgagcgagtc 75900
gttcctgacg gcctcgcgtc ggcgtccagg ccccctccgc atcgccttct cctgcactcc 75960
gcccaagcct cacgaccggc tgaacgccga ggtcaagcag acgttcctgg ctgcggtcgc 76020
caacttcgag gcgctcgggc acacggtcac cgagatcgac catggtctgg acggcatctt 76080
cgacagcttc atccgagtca tcgcggccaa taccgcattg tcggtgacgc agaccgtccc 76140
gttggggagc ctgaacctgc tggaacccaa cacgctcgga ctggcccagc gcgggtgggg 76200
gctcagcgcg atggactact gcgaggcgat caaccacctg cgtaccaccg ccgcactgag 76260
catggcccga tggaccgagg acttcgacgt cctgctcacg cccaccctca cggacctgcc 76320
gccgttgacg ggacagatgc cgtcgtacga cggtgacttg gacgcctgct atctgcacat 76380
gctcggacac aatgccttca cgtatccgtt caacgtcacc ggccagccgg cgctctccat 76440
cccgtgcgga tggagcacct ccggcttgcc catcggcttg cagatcatcg ggggcatggg 76500
ccaagaggcc cgcgtgctgg cgttggccgc cgcctatgag gaggcacatc cctgggcggc 76560
gcgcaagccg ccgctgtgag cctcgaccaa cccctcctgt cgtggttgaa aataagcccg 76620
gtgccggtgg catgattgct ggcgatctag tggcgcgcgc gaagcctgat gggaagacat 76680
tcttcgtcgg tggcaatggc ccactagcta ccaaaaggag gccccccggc tctgccgggg 76740
aggcagtaga agtttgactt ttcaggaggt tgtccaccgt agagactttc aatcgtgagc 76800
cgccaagcaa acgaaaggaa gaaacacgat ggacaagtat gaaagtttga gtcacacggc 76860
ctgggattgc aaatatcacg tggtcttcat ccccaagtgt cgaaggcgaa cgctgtacaa 76920
gcagctacgc gaacacctgg gtgaggtatt cagaaagttg gccgcgcaaa aggaatgcag 76980
ggttgaagag gggcatctga tgcccgatca cgtgcacatg atgctgtcga tcccaccgaa 77040
gtactcggtg tcgcaggtgg tgggctttat caaaggcaag agcgcaatcc accttgcgcg 77100
ggtgtacgga gagcgaaagc gcaacttcgt gggtcagcac ttctgggcgc gagggtactt 77160
cgtctcgacg gtgggacgcg acgaagcggt gatacgggag tacatccgaa accaggagaa 77220
ggaagacgag aggttggacc aactcggtct atggaggtga gtagccaccg ataggtggcc 77280
ccaagaatga ggggccgcgt tagcgacccc ttacagccgc tttgagcggc tcacatcccg 77340
aatgcccccg gctttgccgg gggatggtta cttctgaacg cggctatcta tgacaagatg 77400
ccatatgatc ccgcgaagtc ctttgcgcca gtagctgggt tgggaaaatc accgcttctc 77460
ttggtcaccc gtaatggcct tggcgtcgac agcgtcgcgg agttggtgtc ggtgatgaag 77520
acccggcaac tgtctatggc ctcagccgga atcggcaatg tgacccatct tgccggcgag 77580
tatgtggcgg ctctgatggg gtacaaggtc acgcacgtac cgtttagcgg aagcgcacca 77640
gctatcttgg gcctgatcgg cgataacgtt gacgtcatgt tcgacgcgct gccgtcttca 77700
atgcatcaag ccaaagctgg aaaaatccgc cccttggcca ttcttgatga ccgacggttt 77760
ccgcttttgc cggacgtgcc cacactaaaa gaatcgggat acgccaatgg cgagaccagc 77820
gcgtggattg cgatcgtggc gccggcgcag acgccgcgtt ctgctgtatt ggaaattaac 77880
aaggctgtga atgatgcgtt gctcaaacca gaagtagcgc agaaatttca ctcgattgga 77940
gaacaaccga tggcagggac acccgaagac cttggcaact tcatggcggc cgagcgtgcg 78000
cgttggttgc cacttgtgaa atcgcttggc gtgaagctcg actagctacc aaaaggaggc 78060
cccccggctc tgccggggag gcagtagaag tttgactttt caggaggttg tccaccgtag 78120
agactttcaa tcgtgagccg ccaagcaaac gaaaggaaga aacacgatgg acaagtatga 78180
aagtttgagt cacacggcct gggattgcaa atatcacgtg gtcttcatcc ccaagtgtcg 78240
aaggcgaacg ctgtacaagc agctacgcga acacctgggt gaggtattca gaaagttggc 78300
cgcgcaaaag gaatgcaggg ttgaagaggg gcatctgatg cccgatcacg tgcacatgat 78360
gctgtcgatc ccaccgaagt actcggtgtc gcaggtggtg ggctttatca aaggcaagag 78420
cgcaatccac cttgcgcggg tgtacggaga gcgaaagcgc aacttcgtgg gtcagcactt 78480
ctgggcgcga gggtacttcg tctcgacggt gggacgcgac gaagcggtga tacgggagta 78540
catccgaaac caggagaagg aagacgagag gttggaccaa ctcggtctat ggaggtgagt 78600
agccaccgat aggtggcccc aagaatgagg ggccgcgtta gcgacccctt acagccgctt 78660
tgagcggctc acatcccgaa tgcccccggc tttgccgggg gatggttact atccaggcca 78720
acaatccact ccaaaaaagg cgcaggctac ggcgacttct ggagcccatt ggaatggtgt 78780
cgcctaagaa actcgaccga tcgcgagagt gccagtttgg cggcttcagg cgcgaacccg 78840
aatgaacgga ccgagttgaa gaacccgtgg ccagcctttt cataggtaaa gaactcatgc 78900
aaacctggag ggatagatag ggctcgcatc aaaatttcct gcacctccgc tggaatatgc 78960
gcatccgaat ccccaatgtg aacttgcgtt gggcacttca gggccgcgag atccgcttga 79020
tagtcctgca ccttgactgg ataaaaagac acgctgctgt cgatgccgcc gatcgccgaa 79080
agctgaaggg cgtacttacc tcccaagcag aagccgagga cgctgacgct tccgtcgcag 79140
cgtggatcac ttgcgagcgc ctcgaccgcc accctcgcgt cgtgcagtgc gacttgatca 79200
tccattcgtt gccaaaggga gatggctatc tcgcggtccg actcggagta cgtcaattcg 79260
acgccgggct cgatctggct gaaaatatcc ggggccagca ctacaaaacc ttcacgagcg 79320
aactggtcgg ctgccaatct catggcgtta ttgatcccga agatctcggg cagcaggacg 79380
acgccttgtt gcttctccgg tgagccggac gagatgtagc cgcccatcgc accgtgggag 79440
gttttcattt cgaagttgaa gtttttcatt gtttccgcga ataaaagttg atcttgtcca 79500
agtcgggctc gacaccaagc ccgacacctt gcgggactac gacttcgtat tccctgtact 79560
gtgtagcggt cgtagccaag tcgtcggcca gcaaaagcgg accgaccagt tcgcaatccc 79620
aggcgagcgt tggaagcgcg ctgaacaact gaagcgctgc ggccgtgcca atcgagcttt 79680
cgagcgccat tccgccgtaa atgggaatcc cggcggcctc tgcaatggcg gcagccttgt 79740
acgcccgccg gattcctccg cccttgccga ctttcacgcc gtaaatcccc gtagcaccag 79800
ccttaaagca cgtaaaggca tcgcggaagt cccataggct ttcatccgcc gcaatgggta 79860
tgttcagcct catcgaaagg tttgcaaggc cctgcagctg ccacctgggc aaaggctgct 79920
cgatcatggc gacgccggcg tcctgcagtc gaggcaaata ctgcgtacat gttgggaagt 79980
cccacgcctg gttcagatcg accgtgacgg tcgcccgagg atccatcgca tgaatcgctt 80040
tgacggtttg gactacccgc tccgtttcag aacgggcgtc actgagcccc atctttatct 80100
tgaaggtgcg gtgtcgacgg cgctcgatct gacgctcggc atcttcaatg tccacgctgg 80160
cgttgcccga aacaagcacc cacaagaccg gcatcgattc ccgaacccgc ccgccgtaaa 80220
acgcactgac gggcagaccc aggatccggc ccacggcatc gtgcaagaca acgtccacgg 80280
ctgccttggc aaagaaattg gcggacgcca cgcgatccat caagtgcagt aaagtttcgt 80340
gcccgaagag attcgcgcct atcagcgcgg gggccagata tcgatccacg acctccttca 80400
tggtttcgat cgactccccc ccccaaaacg gtgttcccgc gggagtgccc ccttccccgt 80460
acgcgacgac tccgctggaa gtacgcaacg tcataaagag gagggactgg tgcgcatgcg 80520
aagagttcga atggttgtgt accttcttga gaggcaggtc gatgatgcga gtctcgatac 80580
tctcaatggt ttcaataatt tgcgcgttca ttgtgtactc cggcgatata gcgatttgag 80640
ctatgtcgaa tgatctggcc tcgtcccgcc ccacgcattg gcaatcaggc gtctgataat 80700
ttgggcgtcc agagggacgg ggtttgggta gggcttggcc attactgcgg aggtgacgac 80760
gtcgatgtcc gtctccttca tccccagctc ccgcaagccc acgggtacgc cgctgttttt 80820
tgctatgtcg taaatgcgcc cggccggatc acccccgccc atggcccgct tcatgacgct 80880
catggccttg tcgatcttgg aagcgttgta agcgagcgca tgcggtagca ccactgcatg 80940
tgtctcggcg tggggtaggt tgagtgctcc tccgagcgtg tggcaaagtt tgtggtgaag 81000
gcccatggag gtggatgcca ggcaaatggc actcaaccac gatccataaa gtgcctcgac 81060
ccgaacctct ggatcggcaa ggtcgtcacg aagcttcggc aggcttctaa acaacgcggc 81120
gatccctaac tccgcgaacg tgtcgataat tggatttgcg tcgcttgcgt aaagcgcctc 81180
taccgcatga gccatggcat taatgccact tgaaacggac atcgtcagag gaagcgagaa 81240
cgtgagatct acgtcgtaga tcacggtttc cggtagcact ttggccgagc gctgcgtggt 81300
tttgacgccg cgctcgctct ggccaagaat gggggtcatc tccgagcccg cataggtagt 81360
cggtatcacc aactgcggca ggtccgtatg cagtgcgatg gccttggaaa gcccgatcgc 81420
cgatcccccg cccaacgcca ccaccccatc cgcccctaac ctgtgcacat gcgccagagc 81480
tgtctcggtc acttcgacgg gggtgtgcat gacggcgccg gcgaaaacgc caacgcaagc 81540
ggagccaaca agcgaccgta tttgctcagc ttgctctgga acgcttgcca cgatcaggac 81600
tcgtttgcac cccagccgtt cgacctcggc agcgacctcg gaaacagtgc ctcggccgaa 81660
gcggacccgg ctcggcatgc cctggtagag gaaatctttc atcatctgct cgcctacgcg 81720
accgagttcg catagagcgg actgtcgtcg gttggaatat tgaacttgcc gtcaaataca 81780
gctttaatcc agggctggat ggtcacgaag gaacgcagtc cggctcgcgt gaacggatcg 81840
ttctccacga actggcgggc ctcctctaag tcgctcacct caacgatgta gctgccaccc 81900
cgaactgatt tcccgtcgtc ggtaaaagtt gctcccgccg ctagtacttt ggccgcaagg 81960
ccttccatgt atttgcggtg ctcggacacg accgcttggc gtttcgcaac tgtgcccggc 82020
aagtcttgaa aaaaaatcgc tacatacatt gagatatctc cttaaaaaac ggctgcatcg 82080
ctgataactt gtagaccgga atcacgcgcg tggaatcgat tgatctggat cctccagcac 82140
tttgcgacag accgccgcgc cgcggtgcgc gcagcggttg acccagacgc tgagctttcc 82200
ctcttttgtg cgcgttacaa ctaccggcgt gtcgcccacg aacgtggact tgaaatctcc 82260
gggctgcggg atctcggcct ccaaggccac gaaactccag gttggcccgc gatagatgcg 82320
ttgtagttcg agttcgtaaa cctcttggga acggtatacg tccaaaggca cacgggtccc 82380
atcatgtgtt gggaaggtca cgggcttgat atccatctct acgttcctca aatggcgatc 82440
cagcgaggga tggatcgcgt ctccacagct tcttgccaac gcggcaggcc ggcgagcctt 82500
gcgcgtcggg cttcagtgat gcgcggccgt tcaggccgga tcgagaacga aatccttctt 82560
gaggaccttc acgcccccgc gctcctgcaa cttgtggacc aaggcggggc gcacggcctc 82620
gacgcagtcg ctgtcgaccc acttctcgcc cgcgaagtag gcctgcgtcg tgtgctccaa 82680
aaagcccggc gcgcgcacct tgaagtgcac gtgcgcgggt cgccacggat ggcggcccat 82740
cagctccagc atcttgccgc tagggccctg gtggggaatc atgtagggca ccggcaccac 82800
ggtacggatg ctgaaggcgc cggtcgaatc gatgatcttc ttgccgcgat agaagtccgg 82860
tgaaatctca gggttgaatc cactgtattc cccttcgtgg tcggagtgcc agatgtacag 82920
ctcggcacct tcgaccggct tgcccgccaa atcgacaacc ttgccctcga taatcagctc 82980
ctcgccgcca tcgccacgtg tcttgatcct gtccgtgatc gtgggaatgg actccaggaa 83040
atagggtccc tccacgttgg tcacggagcc cttccggtgg ttcatctcgt tgtcggtgat 83100
ggttgcgttg aaccagagat cgcacaccaa gggaatttcg tattccggag ccttggcgta 83160
ttgcatgagg aagcccacgc cagcgcggta ctcttcaaac gtcacgtcgt ggcgcttcag 83220
aacttcgcga accgccttga cgatttcgct gatcactgtc tctgcccggt ttgccatatt 83280
cttgtctcct gtcgtggtgg atggaacgaa tgcagaatct accgggcggg gacgattcaa 83340
tccaatacca tctttggcat cgggatgacc agaaaggtat cgcggcagcg gcgctggggt 83400
acaattgggt atggagctac gccaccttcg ttatttcgta accgtcgcag aggagcgcag 83460
cttccgtcgc gcctcgctga gacttcatat ctctcagccg ccgctgtcgc gcctaataaa 83520
gcagcttgag gagcaagttg gtgctcagct attcgatcga accacgaccg gggtcgaact 83580
caccgcggca ggagaggtct ttttggaggg cgcaaacaag gctctgcgga taacaagtga 83640
cgcggtcgaa agatcgcgtc gtgctgcaaa gggcgacttt ggccacctcg aagtcgccta 83700
ttacggctcc gtgatcttcg gcaccatccc tcatctgatc ggcgcctatc gtgacgcaca 83760
accccgcgtc acagttcgtc tgtcgcacat gacgaaggat tggcaaatcc gagctctgcg 83820
cgatgggtgg cttgacatcg ggtttgcccg ctattatcga gacgaaccgg acatcgcatc 83880
agaggtggtg gtgagcgagc cggtggtact tgcggtgccg tcgacgcacc cattggccaa 83940
ccgcaccgtc gtgcccatgt cggccctgcg agatgagtgc atgatcgtct accctgcggc 84000
gtctcgtcca agctttgccg atgaggtcat gctcttttgc gatcaggcgg ggttcacacc 84060
tcgcatcgtt caggaaacgg aagatcccat ggcttgcctc gctctagtat ccggcggagt 84120
aggcatcgca ctagtcccaa tatccacagt caatatccgt ctaccgcggg ttgcttttct 84180
caaagtcact gatcctgagc ccacaagtag cctgtgctgc gtctaccgaa ggaacgacca 84240
gaggccactt ctctccggtt ttctagctgt ggtgcgcgcc ttgaatctga acgcactgaa 84300
taacaacgcg ttcgaggagc aaagtcgatt ggtcgagctc ggtcacgaca gtcgaggccg 84360
tcgtaaggcc aacatttgac ctctgcggcg tatgttcaac gccccattcg ccggtattga 84420
atgtagattt tcaatgccgg ccgactgcta gagcgtcgga cccgaagggc tgcacgcgtg 84480
gcagggcatg ggtcgcgtcg ctgttttaga gggcgagcga ccctctcgcc acgagagccc 84540
actctgagcg ccgaatctca aggcagccgg acgcagtgaa gtccagctgc gcgccccggt 84600
cggatgcgct tggatcgaag ccagtggcgt catccgatct tcgaacctgt ggggctctgc 84660
acgtctcgag atgcattcga ccgctgccgt tccagctgcc gccgtcgagc gatttcagtc 84720
cgccacgtag acctgcgtac ctcagtagtt ccggcggaac gactgtcccc gtgggtgccg 84780
gcctccggta tggcgccgtg cctgaaaagg tattggcctg cgcattgcgg agagcctaaa 84840
gtcgggtgga ccctgatggc cacccaaact cccccactta tggccacctc aaactccccc 84900
acctgaactg atcggggata ggggttaaac gccggcgccg tcggcacctg ttggcaggac 84960
attgatccgg tcttcctcga gggaaggcca aggagtgaat gtcttgaagt ccaaccaacg 85020
cgccaccata gagacactgc tggagcgcaa tacatcgcaa cgcgagatcg cccgcatcac 85080
gggcatcgac cgcaagacgg tcaggagcta tcaccaacgc tggctggagc aactgcagtc 85140
aaattccccc ggggtggcca ccggctcggc ccagcaaatt cccccaccct ggccaccggc 85200
tgctgtgccg gtggccacct ccctgtgcga accctggcgc gagttcatcg aagcccagtt 85260
gcgtctgaag cgcaacgcca tggccatctt ccaggacctg gtcgaccagc acggatttac 85320
tggtcagtac aactcggtca aacgcttctg tgcaaagctg cgccacaagg agcccgagca 85380
gttcgaccgc ctgtcctttc tgcctgggga ggagatgcag gtggactacg gcgagggcgc 85440
gcccacccgc gtgccgggca gcgagcggta ccgcaagccc cgcctgttcg tggccacgct 85500
gcgctactcg cgcgccagtt tccgctgcgt ggtctggaag tccagccagc aaatctgggc 85560
cgagttgcac gagcaggctc tgcggtactt cggtggctgc ccgcagtacg tggttctgga 85620
caatctgaag gaaggcgtct tcaagcccga cctgtacgag cccgaactca acaaagtcta 85680
cgccgccacc ctggcgcact atggcgtggt ggccgacccg gcgcgggtgc gagaccccaa 85740
ccgcaagggc acggtggagc atgccattgg ccacacccag gccacggcct tgaagggccg 85800
gcgctttgag tccatcgagg cccagaacga gttcctggcg cactgggaga agagctgggc 85860
agccaagcgc atccacggca ccgagcggcg ccaggtgcag gccatgttcg aggaggagcg 85920
cagccacctc aaacctctgc cgctcctggg catgcagtat ttcgaggagg cggtgcgcac 85980
cgtctgcgac gacagctgcg tgcgggtgga tcacagcagc tacgccgctc gcccggcgaa 86040
catcggctcc aaggtgctgg tgcgcatcta tgcccagcgc atcgagatcc gcgatctgca 86100
aaccggcgcc ctgctgcgca cccacgccaa agccgagcgc cccggcacgg tggtgctgcc 86160
catggaggag cgggtattca atccttcgcg cgagacccgt ctgatcctgc gtcaggctgg 86220
cgagatcggt gagcacgcca aacggctgtg tgagctgctc tttgccatcg agggtcgggt 86280
gggccagcgc aagctctggg gcatcgtcag cctggccagg cgatacccag cgcactgcgt 86340
cgacaccgcc tgcgcacagg ccctggagca ggggatttac agctacaagc gcgtgctggc 86400
tttgaccgag gccatctttg cccaggcgct caacgccatc gagacccagt ccagcggtgc 86460
gcccgccggc ggcgggcgca cgctgaccca gcagcacgag ctcatccgcg acgccgatga 86520
gtacgccgat ctgttcgcgc acgctgcggc cgtcacagcc acgagcacgc tcgagtccgc 86580
cacggcgaac accaccacca acaacaccaa caacgcacct ggaattcagc catgaacatg 86640
atcgagatcg aacgcgcgct gcgcgagctg cgtctgtccg gcattgccga gaccctgtcc 86700
acccgcgtga tgcaggccca ggccgcgcag gagcccttcc tggagacctt tggggccatg 86760
ctgcaagacg aactggaccg ccggcgctcg cgtctgaccg agcgccgctt caagcgctcg 86820
ggcctggatg agcggccctc gctggctgac ttcgactggc gtttcaaccc gaagctgccg 86880
cgcagcgcct gcttcgagtt gcataccctg aagttcatcg gcgagggcgc caatgcgctg 86940
atcatcggca agcccggtac cggcaagagc catgtggcca aggccgtggc ctaccaggca 87000
acgctgcagg gatacgacgc gcgttacctg gaagccgaca ccgagtttgc tcgctacgcg 87060
ttggccagcg actcagagcg caccgagctg ctcaaggact gggtctcacc ggacctgatc 87120
atcctcgatg acctgttcct ggccagacgc atcagcgagc acgcagccga ggtgctgcag 87180
gccatcgtgc accagcgcta caagctgcgc cgctccatcg tcatcacatc caaccgcgtg 87240
gtgcaggact ggggcaaata tctgggcgac gccaccatgg ccaccaccat cctggaccgc 87300
ctcatgcacc gctgcgcaat gctggagttc gagggcaaga gctaccgcct caaggaggct 87360
gccgcacgca tcgccatcac acccgagtcg tcataatctg gtcgtcctgc ctgggggaat 87420
ttggggtggc caagggtggg ggaatttgac ctggccatcg gggggtggat cgttgtttgg 87480
agaacgtctt gaatccaccc agctcaatgc gccgccgttc cgtgctcgca ggtctttctt 87540
tcgcctgcat ggccaacggc gcctcatggg cgcaggcctc ctatccgagc cgatccatca 87600
aaatcatcgt cccgtttgga gccggcgcat cagctgacct cataacgcgc cgcgtcgcag 87660
aggctatggc gacctcactc gggcaagccg tgatcgtaga aaaccgcccc ggcgctacgg 87720
gcaatctggg tacgggagta gctgctaagg cggagcctga cggctacacc cttgttgttg 87780
cttcgactcc caacttcgcc atcaacacgg cactctatcg ccaactcccc ttcgacgtcg 87840
acgctgactt cagcccgatc tctctgatgg ccgaagcgcc caacgtgatt gcggtaaacc 87900
cctctacagg catcacgtcc atgggggatc tggtcgttgc ggcccgcagg gcccctggtg 87960
ggcttgcata cacctcagcc acagtcggta gcacgggaca cctgcttggt gagctactag 88020
ctgcccgcgc cggaatcagg ctgatacatg tgcccgacaa ggacccgatt acgctggttc 88080
tcggcggcca cgccccgatg acaattttca ccactgcttc agtccttcca caccttcggt 88140
cggggcgact gaaggcattg gcggttacct ccgcgagccg gcttgccgtc gcaccagacg 88200
ttccaacgtt gctcgaaatg aagggaatcg acatcagtgg gacggcttgg tacgggctgg 88260
tcgccccacg cgcgacgcca gcagaagccc agacgaagct ccgctctgca gtcaagagcg 88320
cactgcaaac cccgcgtgtg ctaagcgcgc tggtggattc gggacacgag gtgcgcttgt 88380
tggaaggatc ggatttcgga gcgtttatgc gcgccgaacg cgttaagtgg gcggaggctg 88440
tgaggctctc cggagcgaga cccgagtgac cccagtcggg cggaacggag gcccagtgac 88500
gttccccccg atttccggac tttttaaaac taga 88534
<210> 2
<211> 474
<212> PRT
<213> LR2014-1(Diaphorobacter sp. LR2014-1)
<400> 2
Met Thr Ala Thr Asp Glu Leu Tyr Tyr Leu Pro Val Thr Glu Leu Ser
1 5 10 15
Glu Leu Ile Ala Glu Arg Arg Leu Ala Pro Ser Glu Leu Met Ser Ala
20 25 30
Val Ile Ala Arg Ala Glu Glu Val Asn Pro Lys Leu Asn Ala Leu Val
35 40 45
Ala Gln Arg Phe Glu Ala Ala Thr Arg Glu Ala Ala Ala Ala Asp Asn
50 55 60
Glu Pro Ser Arg Gly Val Leu His Gly Ile Pro Ile Thr Leu Lys Asp
65 70 75 80
Leu Ala Tyr Glu Thr Pro Asp Leu Pro Ser Thr Tyr Gly Ser Arg Ala
85 90 95
Phe Ala Gly Tyr Glu Pro Gly Phe Glu Thr Val Val Gly Gln Arg Leu
100 105 110
Arg Ala Ala Gly Thr Ile Ala Ile Gly Arg Thr Asn Ser Pro Glu Phe
115 120 125
Gly Leu Thr Asn Thr Cys Glu Ser Ala Gln Phe Gly Pro Thr Ser Asn
130 135 140
Pro Trp Arg Pro Glu His Thr Pro Gly Gly Ser Ser Gly Gly Ala Gly
145 150 155 160
Ala Ala Val Ala Ala Gly Ile Ala Pro Leu Ala Ala Ala Asn Asp Gly
165 170 175
Gly Gly Ser Cys Arg Val Pro Ala Ser Ser Cys Gly Val Val Gly Leu
180 185 190
Lys Pro Ser Arg Gly Arg Val Pro Trp Ala Pro Thr Ser Tyr Glu Tyr
195 200 205
Trp Ala Gly Phe Ala Thr Asn Gly Pro Ile Ala Arg Thr Val Glu Asp
210 215 220
Val Ala Leu Leu Leu Asp Ala Met Ser Gly Pro Val Val Gly Glu Pro
225 230 235 240
Tyr Gly Leu Pro Ala Pro Ser Glu Ser Phe Leu Thr Ala Ser Arg Arg
245 250 255
Arg Pro Gly Pro Leu Arg Ile Ala Phe Ser Cys Thr Pro Pro Lys Pro
260 265 270
His Asp Arg Leu Asn Ala Glu Val Lys Gln Thr Phe Leu Ala Ala Val
275 280 285
Ala Asn Phe Glu Ala Leu Gly His Thr Val Thr Glu Ile Asp His Gly
290 295 300
Leu Asp Gly Ile Phe Asp Ser Phe Ile Arg Val Ile Ala Ala Asn Thr
305 310 315 320
Ala Leu Ser Val Thr Gln Thr Val Pro Leu Gly Ser Leu Asn Leu Leu
325 330 335
Glu Pro Asn Thr Leu Gly Leu Ala Gln Arg Gly Trp Gly Leu Ser Ala
340 345 350
Met Asp Tyr Cys Glu Ala Ile Asn His Leu Arg Thr Thr Ala Ala Leu
355 360 365
Ser Met Ala Arg Trp Thr Glu Asp Phe Asp Val Leu Leu Thr Pro Thr
370 375 380
Leu Thr Asp Leu Pro Pro Leu Thr Gly Gln Met Pro Ser Tyr Asp Gly
385 390 395 400
Asp Leu Asp Ala Cys Tyr Leu His Met Leu Gly His Asn Ala Phe Thr
405 410 415
Tyr Pro Phe Asn Val Thr Gly Gln Pro Ala Leu Ser Ile Pro Cys Gly
420 425 430
Trp Ser Thr Ser Gly Leu Pro Ile Gly Leu Gln Ile Ile Gly Gly Met
435 440 445
Gly Gln Glu Ala Arg Val Leu Ala Leu Ala Ala Ala Tyr Glu Glu Ala
450 455 460
His Pro Trp Ala Ala Arg Lys Pro Pro Leu
465 470

Claims (7)

1. A trichlorocarban metabolism plasmid pDCA-TCC is characterized in that the nucleotide sequence is SEQ ID NO. 1.
2. A triclocarban-degrading bacterium containing the triclocarban metabolic plasmid pDCA-TCC of claim 1.
3. The triclocarban-degrading bacterium according to claim 2, characterized in that the host bacterium is Pseudomonas putida KT 2440.
4. Use of the triclocarban metabolic plasmid pDCA-TCC of claim 1 for degrading chloroaniline derivatives.
5. The use according to claim 4, wherein said chloroaniline derivative is selected from any one of triclocarban, anilazine, chlorpropham, forchlorfenuron and diflubenzuron.
6. Use of the triclocarban-degrading bacterium according to claim 2 or 3 for degrading a chloroaniline derivative.
7. The use according to claim 6, wherein said chloroaniline derivative is selected from any one of triclocarban, anilazine, chlorpropham, forchlorfenuron and diflubenzuron.
CN202210413124.7A 2022-04-19 2022-04-19 Trichlorocarban metabolic plasmid pDCA-TCC and application thereof Pending CN114807207A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115537366A (en) * 2022-12-05 2022-12-30 东北农业大学 Microbial agent for biochar coupling synergistic degradation of TCC (TCC) and preparation method thereof

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Publication number Priority date Publication date Assignee Title
CN107312731A (en) * 2017-07-13 2017-11-03 中国科学院生态环境研究中心 One plant of triclocarban degradation bacteria and its application
CN109337877A (en) * 2018-10-31 2019-02-15 安徽农业大学 The application of chlorophenesic acid degrading enzyme TcpA and its encoding gene and production bacterium
CN112625985A (en) * 2020-12-04 2021-04-09 浙江工业大学 Genetically engineered bacterium for high yield of beta-alanine and co-culture preparation of D-pantothenic acid

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CN107312731A (en) * 2017-07-13 2017-11-03 中国科学院生态环境研究中心 One plant of triclocarban degradation bacteria and its application
CN109337877A (en) * 2018-10-31 2019-02-15 安徽农业大学 The application of chlorophenesic acid degrading enzyme TcpA and its encoding gene and production bacterium
CN112625985A (en) * 2020-12-04 2021-04-09 浙江工业大学 Genetically engineered bacterium for high yield of beta-alanine and co-culture preparation of D-pantothenic acid

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Cited By (1)

* Cited by examiner, † Cited by third party
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CN115537366A (en) * 2022-12-05 2022-12-30 东北农业大学 Microbial agent for biochar coupling synergistic degradation of TCC (TCC) and preparation method thereof

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