CN114797696B - Extreme manufacturing equipment of three-dimensional sphere structure of microdroplet and use method - Google Patents

Extreme manufacturing equipment of three-dimensional sphere structure of microdroplet and use method Download PDF

Info

Publication number
CN114797696B
CN114797696B CN202210202943.7A CN202210202943A CN114797696B CN 114797696 B CN114797696 B CN 114797696B CN 202210202943 A CN202210202943 A CN 202210202943A CN 114797696 B CN114797696 B CN 114797696B
Authority
CN
China
Prior art keywords
droplet
target
micro
laser
droplets
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202210202943.7A
Other languages
Chinese (zh)
Other versions
CN114797696A (en
Inventor
郭金坤
凌进中
袁影
王晓蕊
刘鑫
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Xidian University
Original Assignee
Xidian University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Xidian University filed Critical Xidian University
Priority to CN202210202943.7A priority Critical patent/CN114797696B/en
Publication of CN114797696A publication Critical patent/CN114797696A/en
Application granted granted Critical
Publication of CN114797696B publication Critical patent/CN114797696B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J13/00—Colloid chemistry, e.g. the production of colloidal materials or their solutions, not otherwise provided for; Making microcapsules or microballoons
    • B01J13/02—Making microcapsules or microballoons
    • B01J13/04—Making microcapsules or microballoons by physical processes, e.g. drying, spraying
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0241—Drop counters; Drop formers
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P10/00—Technologies related to metal processing
    • Y02P10/25—Process efficiency

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dispersion Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)

Abstract

The invention belongs to the technical field of soft condensed state substance manufacturing, and particularly relates to extreme manufacturing equipment of a three-dimensional sphere structure of microdroplets and a using method thereof. The system comprises a laser micro-nano processing system, a micro-fluidic chip system and a real-time observation system. The using method comprises the following steps: step 1: obtaining target microdroplets, and enabling a tunable laser to emit laser spots; step 2: striking the focused laser spot to the boundary of the target droplet; step 3: exposing for a plurality of seconds by laser, injecting the aqueous solution into the target droplet to form sub-droplets, and completing the sphere structure assembly of the target droplet in the interlayer cavity; step 4: synchronizing with the step 3, illuminating a light source, and observing by a CCD camera; step 5: the injector moves the processed soft matter droplets into a droplet collector. The invention can control the micro-nano particles to be self-assembled into different three-dimensional morphological structures in the micro-droplets, and has important scientific and technical values for promoting the progress of extreme manufacturing technology of the micro-droplet sphere structure.

Description

Extreme manufacturing equipment of three-dimensional sphere structure of microdroplet and use method
Technical Field
The invention belongs to the technical field of soft condensed state substance manufacturing, and particularly relates to extreme manufacturing equipment of a three-dimensional sphere structure of microdroplets and a using method thereof.
Background
Soft materials, also known as soft condensed materials, include liquid crystals, colloids, polymers, particulate materials, living system materials (e.g., DNA, proteins, cell membranes), emulsions, and the like, are widely present in nature, life, daily life, and production and have profound effects. In recent decades, micro-nano processing technology for solid materials has realized integration of complex electronic functions on micrometer-scale electronic devices, contributing to rapid development of modern electronic industry, particularly semiconductor industry. Because the macroscopic characteristics of the intelligent flexible material depend on the chemical components and the microstructure, the micro-nano processing technology for liquid and soft material has important scientific and technical values in the fields of photonics, optoelectronics, flexible electronics, intelligent sensing, chemical industry, medicine, biopharmaceuticals and the like. But in comparison its development is rather delayed.
However, at present, droplet microfluidic technology is generally adopted for micro-nano processing of fluids and soft substances, and the structural design of a microfluidic chip is limited by the special physical properties of fluidity, interfacial tension, diffusion action and the like of the materials, so that only a few simpler droplet structures can be assembled: such as the nesting of sub-droplets of one material in droplets of another material, does not allow for flexible assembly of the droplet-sphere structure.
The laser injection technology is an emerging micro-nano processing technology for fluids and soft substances in recent years, and the main principle of the technology is to inject an aqueous solution into liquid crystal droplets by utilizing the interaction of light and substances and utilizing Gaussian beams to irradiate the surfaces of the liquid crystal (soft substance) droplets in the aqueous solution to form mechanical force so as to form sub-droplets. The technology expands the laser micro-nano manufacturing to the extreme manufacturing field of fluid and soft substances, and shows the flexibility and complexity of the far-ultra-traditional microfluidic technology.
However, the droplet-sphere structure assembled by this technique is now relatively simple: the micro-nano particles are distributed in the liquid crystal microdroplet along the radial direction of the sphere, so that the possibility of subsequent application and development is greatly restricted. If the potential of self-organization of the material can be further developed, the micro-nano particles are controlled to be self-assembled into different three-dimensional morphological structures in the micro-droplets, and the method has important scientific and technical values for promoting the progress of the extreme manufacturing technology of the microsphere structure and the development of potential application.
Disclosure of Invention
Aiming at the problems existing in the prior art, the invention constructs an extreme manufacturing system of a three-dimensional spherical structure of microdroplets, carries out micro-nano processing on soft material microdroplets with different topological defect structures through a laser injection system, and self-organizes injected micro-nano particles into a pre-designed three-dimensional crystal structure in the soft material microdroplets by virtue of the diversity of the topological defect structures of the soft material microdroplets, thereby realizing the purpose of assembling the complex spherical structure of the soft material microdroplets; the extreme manufacturing system of the microsphere structure can be used for processing polynuclear soft material microspheres (water-in-oil-in-water), and can also be used for simply assembling the polynuclear oil-in-water (O/W/O) microspheres by using an aqueous solution and soft material (oil phase) in the system.
In order to achieve the above purpose, the technical scheme adopted by the invention is as follows:
the utility model provides an extreme manufacturing equipment of three-dimensional spheroid structure of microdroplet, includes laser micro-nano processing system, micro-fluidic chip system, real-time observation system, laser micro-nano processing system includes tunable laser, aperture diaphragm, dichroscope and automatically controlled translation platform, aperture diaphragm sets up in tunable laser's left side, the dichroscope sets up in aperture diaphragm's left side, automatically controlled translation platform sets up in the top of dichroscope, micro-fluidic chip system sets up on automatically controlled translation platform, real-time observation system includes light source subsystem and camera subsystem, light source subsystem sets up in micro-fluidic chip system's top, camera subsystem communicates with each other with the dichroscope.
Preferably, the microfluidic chip system comprises an upper substrate and a lower substrate, a cavity interlayer channel is arranged between the upper substrate and the lower substrate, one end of the cavity interlayer channel is an injection port of soft material droplet aqueous solution, the injection port is connected with an injector through a connecting pipe, the other end of the cavity interlayer channel is an outlet end, and the outlet end is connected with a droplet collector through a connecting pipe.
Preferably, the camera subsystem includes a light source disposed at an uppermost end of the extreme manufacturing system and a condenser lens disposed between the light source and the microfluidic chip system.
Preferably, the camera subsystem comprises a microscope objective, a reflecting mirror and a CCD camera, wherein the microscope objective is arranged between the electric control type translation stage and the dichroic mirror, the reflecting mirror is arranged below the dichroic mirror, and the CCD camera is arranged on the positive side of the reflecting mirror.
A method of using an extreme manufacturing apparatus for a three-dimensional sphere structure of droplets, comprising the steps of:
step 1: the soft material droplets are E7 liquid crystals doped with R811 with different concentrations to obtain target droplets, a micro-fluid peristaltic injection pump is used for controlling an injector to send a micro-drip solution containing the target into a micro-fluid control chip for processing, and then a tunable laser emits a continuous laser beam with a large diameter, and the continuous laser beam is focused by an aperture diaphragm, a dichroic mirror and a micro-objective lens to obtain a small-diameter laser spot;
step 2: the focused laser facula is beaten to the boundary of the target droplet of the interlayer cavity of the micro-fluidic chip;
step 3: exposing the target droplets to the laser for several seconds to generate mechanical forces at the interface of the target droplets, the forces injecting aqueous solution around the target droplets into the droplets to form sub-droplets, the injected sub-droplets spontaneously align along a topology within the droplets, thereby processing the spherical structure of the target droplets within the sandwich cavity;
step 4: during the processing of the step 3, the dynamic structure morphology of the target microdroplet in the microfluidic chip can be observed in real time through the illumination of the light source by the CCD camera;
step 5: the syringe is driven by a microfluidic peristaltic syringe pump and the processed soft matter droplets are moved into a droplet collector and the processing proceeds to the next.
Preferably, in the step 1, the target droplet is doped with 0.1wt% of r811, the doping concentration reaches the first target soft material droplet in a steady state, and the aqueous solution in the step three is doped with 0.2wt% of the surfactant SDS, so that the first target soft material droplet is stably suspended in water, the topology structure of the first target soft material droplet reaches the steady state is a topology line uniformly distributed near the surface of the first target soft material droplet, and the topology lines are connected end to form a ring;
in the third step, the aqueous solution is injected into the first target soft substance droplet by a laser injection system to form a first sub-droplet, the first sub-droplet is captured by a topological line and moves along the space geometric form of the first sub-droplet, and along with the progress of laser injection, the topological line is completely occupied by the sub-droplet particles to form a particle necklace-shaped structure.
Preferably, in the step 1, the target droplet is doped with 0.05wt% of r811, the doping concentration reaches a steady state to be the second target soft material droplet, the topological structure is a topological point, and the aqueous solution in the step three is doped with 0.2wt% of surfactant SDS, so that the second target soft material droplet can be stably suspended in water by sodium lauryl sulfate;
in the third step, when the laser exposure is 5s, the aqueous solution is injected into the second target soft matter droplet to form a second sub-droplet, the second sub-droplet is influenced by the long-range elastic interaction force of the topological point, the first injected second sub-droplet is captured by the topological point and attracts the rest of sub-droplets to form a droplet chain, meanwhile, the liquid crystal molecules in the second target soft matter droplet are arranged along the topological point to be perpendicular to the surface of the second target soft matter droplet, the injected second sub-droplet spontaneously forms a droplet chain under the elastic action of the liquid crystal molecules, and the arrangement direction of the droplet chain in the sphere space in the second target soft matter droplet is consistent with the arrangement direction of the liquid crystal molecules, and the injected second sub-droplet forms a spiral micro-nano particle chain along the arrangement direction of the molecules in the second target soft matter droplet along with the progress of laser injection.
Preferably, the E7 liquid crystal is doped with a heat absorbing material.
Compared with the prior art, the invention has the beneficial effects that:
1. injecting surrounding aqueous solution into the closed soft material droplets by controlling parameters such as exposure position, exposure power, time and the like of laser to form a specific number of sub-droplets (micro-nano size), controlling and inducing the sub-droplets to be arranged into a predesigned ordered structure in a three-dimensional space in the soft material droplets by the elastic action of soft material molecules, so as to achieve the aim of processing the sphere structure of the soft material droplets;
2. the topology of the soft matter droplets in steady state is stable, by means of which the induced and controlled sub-droplets (micro-nano scale) are distributed in three dimensions along a pre-designed crystal structure (aligned with topology and soft matter molecular direction);
3. the diversity of soft material microdroplet topological defect structures (topological points and topological lines of various forms) under different doping concentrations is effectively utilized, and the soft material microdroplet topological defect structures are used as templates to process microdroplet complex sphere structures;
4. the geometric form of the microsphere structure assembled by means of the self-organization of the materials is greatly expanded, and because each microsphere can be independently observed, controlled and execute specific functions, the various microsphere structures provide more possibilities for subsequent application development.
Drawings
The accompanying drawings are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate the invention and together with the embodiments of the invention, serve to explain the invention.
In the drawings:
FIG. 1 is a schematic diagram of the overall optical path structure of an extreme manufacturing system for a three-dimensional sphere structure of droplets according to the present invention;
FIG. 2 is a side view of a portion of a microfluidic chip in an extreme manufacturing system of a three-dimensional sphere structure of droplets according to the present invention;
FIG. 3 is a schematic diagram of several exemplary topological defects exhibited by a soft-material droplet and its corresponding microstructure texture map;
FIG. 4 is a schematic diagram of a topology line assembled droplet sphere within a soft matter droplet of example 2;
FIG. 5 is a schematic diagram of a topology line assembled droplet sphere within a soft matter droplet of example 3;
FIG. 6 is a flow chart of a method of using the droplet three-dimensional sphere structure extremity manufacturing system of the present invention;
in the figure: 1. a tunable laser; 2. an aperture stop; 3. a dichroic mirror; 4. a microobjective; 5. an electronically controlled translation stage; 6. a microfluidic chip; 7. a target droplet; 8. a condenser; 9. observing a light source; 10. a reflecting mirror; 11. a CCD camera; 12. an upper substrate; 13. a lower substrate; 14. a microfluidic chip cavity; 15. an injection port; 16. an outlet end; 17. droplets of soft material to be processed; 18. droplets of the target soft matter in process; 19. droplets of processed soft matter; 20. a microfluidic peristaltic syringe pump; 21. a connecting pipe; 22. a syringe; 23. a droplet collector; 24. target soft matter droplet one; 25. target droplet-topology defect structure; 26. a topological line; 27. first droplet after laser injection processing; 28. sub-drop one; 29. a sphere structure after the first laser processing of the droplet; 30. target soft matter droplet two; 31. topology points; 32. a target droplet two topology defect structure; 33. a second droplet after laser injection processing; 34. a micro-nanoparticle chain; 35. and sub-droplets two.
Detailed Description
The preferred embodiments of the present invention will be described below with reference to the accompanying drawings, it being understood that the preferred embodiments described herein are for illustration and explanation of the present invention only, and are not intended to limit the present invention.
Example 1:
referring to fig. 1 and 2, an extreme manufacturing device of a three-dimensional sphere structure of a droplet comprises a laser micro-nano processing system, a micro-fluidic chip system 6 and a real-time observation system, wherein the laser micro-nano processing system comprises a tunable laser 1, an aperture diaphragm 2, a dichroic mirror 3 and an electric control translation table 5, the aperture diaphragm 2 is arranged on the left side of the tunable laser 1, the dichroic mirror 3 is arranged on the left side of the aperture diaphragm 2, the electric control translation table 5 is arranged above the dichroic mirror 3, the micro-fluidic chip system 6 is arranged on the electric control translation table 5, the soft material droplet is positioned in the micro-fluidic chip system 6, the real-time observation system comprises a light source subsystem and a camera subsystem, and the light source subsystem is arranged above the micro-fluidic chip system 6 and is communicated with the dichroic mirror 3.
The microfluidic chip system 6 comprises an upper substrate 12 and a lower substrate 13, a cavity interlayer channel 14 is arranged between the upper substrate 12 and the lower substrate 13, as shown in fig. 1, a soft substance droplet 17 to be processed, a target soft substance droplet 18 to be processed and a processed soft substance droplet 19 are arranged in the cavity interlayer channel 14, one end of the cavity interlayer channel 14 is an injection port 15 of soft substance micro-drip solution, the injection port 15 is connected with an injector 22 through a connecting pipe 21, the inner diameter of the connecting pipe 21 is 0.1mm-4mm, the injector 22 is connected with a microfluidic peristaltic injection pump 20, the on-off and the flow rate of an aqueous solution in the injector entering the microfluidic chip cavity are precisely controlled by the aid of the cavity interlayer channel, the on-off and the flow rate of the aqueous solution in the injector 22 entering the microfluidic chip system 6 are precisely controlled by the Harvardpump 33, the other end of the cavity interlayer channel 14 is an outlet end 16, the outlet end 16 is connected with a droplet collector 23 through the connecting pipe 21, and the embodiment adopts a glass sample YPP model of Hunan glass to achieve the purpose of processing the droplet.
The thickness of the cavity sandwich channel 14, injection port 15 and outlet port 16 is 0.3-2mm, which is greater than the diameter of the soft matter droplets 18 (typically 0.01-1 mm).
The camera subsystem comprises a light source 9 and a condenser lens 8, the light source 9 is arranged at the uppermost end of the extreme manufacturing system, and the condenser lens 8 is arranged between the light source 9 and the microfluidic chip system 6.
The camera subsystem comprises a microscope objective 4, a reflecting mirror 10 and a CCD camera 11, wherein the microscope objective 4 is arranged between the electric control type translation stage 5 and the dichroic mirror 3, the reflecting mirror 10 is arranged below the dichroic mirror 3, and the CCD camera 11 is arranged on the positive side of the reflecting mirror 10.
Example 2:
a method for assembling a droplet sphere by topological lines within a droplet of a soft material, as shown in figures 1, 2, 3, 4, 6, comprising the steps of:
step 1: the material of the soft material microdroplet is E7 (merck company) liquid crystal doped with R811 (4- (4-hexyloxybenzoyloxy) benzoic acid-R- (+) -2-octyl ester with different concentrations and liquid crystal chiral additives) (sigma-delta-sigma), the target microdroplet 7 is processed by controlling an injector 22 through a micro-fluid peristaltic injection pump 20 to send an aqueous solution containing the target microdroplet 7 into a micro-fluidic chip 6, and then a tunable laser 1 emits a continuous laser beam with large diameter, and after focusing through an aperture diaphragm 2, a dichroic mirror 3 and a microscope objective 4, a laser spot with small diameter is obtained, wherein the typical spot diameter is 5 microns;
step 2: the focused laser light spots are beaten to the boundary of the target droplet 7 of the interlayer cavity 14 of the micro-fluidic chip 6;
step 3: laser exposure for several seconds produces mechanical forces at the interface of the target droplet 7 that inject aqueous solution around the target droplet 7 into it to form sub-droplets that spontaneously align along the topology within the droplet 7, thereby processing the spherical structure of the target droplet 7 within the interlayer cavity 14;
step 4: during the processing in the step 3, the dynamic structural morphology of the target micro-droplet 7 in the microfluidic chip 6 can be observed through the CCD camera 11 by illumination of the light source 9;
in step 1 of this example 1, the target droplet 7 is doped with 0.1wt% of r811, and the doping concentration reaches the first target soft matter droplet 24 at steady state, and the aqueous solution of the third step is doped with 0.2wt% of the surfactant SDS (sodium dodecyl sulfate), so that the first target soft matter droplet 24 is stably suspended in water, the topology of the first target soft matter droplet 24 reaches the steady state is a topology line 26 uniformly distributed near the surface of the first target soft matter droplet, and the topology line 26 is connected end to form a ring and uniformly distributed near the surface of the sphere of the first target soft matter droplet structure 25;
the E7 liquid crystal is doped with 0.1wt% of heat absorbing material (coumarin-6), and the laser spot can be observed when the laser spot works in the laser working area.
In step three of this embodiment 1, the aqueous solution is injected into the first target soft material droplet 24 by the laser injection system to form the first sub-droplet 28, the first sub-droplet 28 is captured by the topological line 26, and moves along the space geometry of the first sub-droplet 28, and as the laser injection proceeds, more and more of the first sub-droplet 28 is injected into the first target droplet 24 until the topological line 26 is completely occupied by the first sub-droplet 28 to form a particle necklace-like sub-structure, and the first target droplet 24 becomes the first droplet laser processed sphere structure 29.
Example 3:
referring to fig. 1, 2, 3, 5, and 6, unlike example 2, in this example 3, the target droplet 7 is doped with 0.05wt% R811 (4- (4-hexyloxybenzoyloxy) benzoic acid-R- (+) -2-octyl ester, a liquid crystal chiral additive) in a manner that the target soft droplet 30 is reached at a steady state, and the topology is a topological point 31, and the aqueous solution of the third step is doped with 0.2wt% of a surfactant SDS (sodium dodecyl sulfate), SDS (sodium dodecyl sulfate) to stably suspend the target soft droplet 30 in water.
The E7 liquid crystal is doped with 0.1wt% of heat absorbing material (coumarin-6), and the laser spot can be observed when the laser spot works in the laser working area.
In the third step, when the laser exposure is 5s, the aqueous solution is injected into the second target soft material droplet 30 to form a second sub-droplet 35, the second sub-droplet 35 is affected by the long-range elastic interaction force of the topological point 31, the first second injected sub-droplet 35 is captured by the topological point 31 and attracts the rest of the sub-droplets to form a droplet chain, meanwhile, the liquid crystal molecules in the second target soft material droplet 30 are arranged along the topological point 31 to be perpendicular to the sphere surface of the second target soft material droplet 30 (as shown by the black line in the circle of the topological defect structure 32 of the second target droplet), the injected sub-droplet 35 spontaneously forms a droplet chain under the elastic action of the liquid crystal molecules, and the arrangement direction of the droplet chain in the sphere space in the second target soft material droplet 30 is consistent with the arrangement direction of the liquid crystal molecules.
As the laser injection proceeds, the injected sub-droplets 35 form a helical chain 34 of micro-nano particles along the direction of molecular alignment of the target soft matter droplets 30.
In the invention, the topology structure of the closed soft matter microdroplet is fixed, sub-droplets are injected into the microdroplet at different high boundary positions in a rhythmic mode by using laser, the loaded microdroplet is arranged along the topology structure of the soft matter microdroplet (the topology points and the topology lines refer to 2 types, not 2 types, and have various distributed topology points and various forms of topology lines), so that the purpose of assembling the microsphere structure of the soft matter microdroplet is realized (each sub-droplet is just the sub-structure in the microdroplet, and the purpose of assembling the microsphere structure is realized by controlling the space distribution of the sub-droplets).
In summary, the present invention controls the injection position and rhythm of the micro-nano particles by controlling the laser, and injects the micro-nano particles into the closed soft material droplets with different topological defect structures in a steady state, so that the micro-nano particles spontaneously organize into a pre-designed particle crystal structure along with the geometric structure of the topological defect structure (topological line or molecular arrangement), thereby realizing the extreme manufacturing of the soft material droplets with complex sphere structures.
The foregoing has shown and described the basic principles, principal features and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, and that the above embodiments and descriptions are merely illustrative of the principles of the present invention, and various changes and modifications may be made without departing from the spirit and scope of the invention, which is defined in the appended claims. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims (4)

1. A method of using an extreme manufacturing apparatus for a three-dimensional sphere structure of droplets, characterized by: the extreme manufacturing equipment comprises a laser micro-nano processing system, a micro-fluidic chip (6) and a real-time observation system, wherein the laser micro-nano processing system comprises a tunable laser (1), an aperture diaphragm (2), a dichroic mirror (3) and an electric control translation table (5), the aperture diaphragm (2) is arranged on the left side of the tunable laser (1), the dichroic mirror (3) is arranged on the left side of the aperture diaphragm (2), the electric control translation table (5) is arranged above the dichroic mirror (3), the micro-fluidic chip (6) is arranged on the electric control translation table (5), the real-time observation system comprises a light source subsystem and a camera subsystem, the light source subsystem is arranged above the micro-fluidic chip (6), and the camera subsystem is communicated with the dichroic mirror (3);
the microfluidic chip (6) comprises an upper substrate (12) and a lower substrate (13), a cavity interlayer channel (14) is arranged between the upper substrate (12) and the lower substrate (13), one end of the cavity interlayer channel (14) is an injection port (15) of soft material droplet aqueous solution, the injection port (15) is connected with an injector (22) through a connecting pipe (21), the other end of the cavity interlayer channel (14) is an outlet end (16), and the outlet end (16) is connected with a droplet collector (23) through the connecting pipe (21);
the camera subsystem comprises a light source (9) and a condenser lens (8), wherein the light source (9) is arranged at the uppermost end of the extreme manufacturing system, and the condenser lens (8) is arranged between the light source (9) and the microfluidic chip (6);
the camera subsystem comprises a microscope objective (4), a reflecting mirror (10) and a CCD (charge coupled device) camera (11), wherein the microscope objective (4) is arranged between the electric control type translation table (5) and the dichroic mirror (3), the reflecting mirror (10) is arranged below the dichroic mirror (3), and the CCD camera (11) is arranged on the positive side of the reflecting mirror (10);
the method for using the extreme manufacturing equipment of the three-dimensional sphere structure of the microdroplet comprises the following steps:
step 1: the soft material droplets are E7 liquid crystals doped with R811 with different concentrations to obtain target droplets (7), an injector (22) is controlled by a micro-fluid peristaltic injection pump (20) to send an aqueous solution containing the target droplets (7) into a micro-fluidic chip (6) for processing, and then a tunable laser (1) emits a continuous laser beam with a large diameter, and the continuous laser beam is focused by an aperture diaphragm (2), a dichroic mirror (3) and a micro-objective lens (4) to obtain a laser spot with a small diameter;
step 2: the focused laser light spots are beaten to the boundary of a target droplet (7) of a cavity interlayer channel (14) of the microfluidic chip (6);
step 3: laser exposure for several seconds generates mechanical forces at the interface of the target droplet (7) that inject an aqueous solution around the target droplet into it to form sub-droplets that spontaneously align along the topology within the target droplet (7) to process the spherical structure of the target droplet (7) within the cavity interlayer channel (14);
step 4: during the processing of the step 3, the dynamic structure morphology of the target microdroplet (7) in the microfluidic chip (6) can be observed in real time through the illumination of the light source (9) and the CCD camera (11);
step 5: the injector (22) is driven by a microfluidic peristaltic syringe pump (20) and the processed soft matter droplets (19) are moved into a droplet collector (23) and processing continues with the next.
2. A method of using an extreme manufacturing apparatus for three-dimensional sphere structures of microdroplets according to claim 1, characterized in that: in the step 1, the target droplet (7) is doped with 0.1wt% of R811, the target soft material droplet (24) reaches a steady state at the doping concentration, the aqueous solution of the step three is doped with 0.2wt% of surfactant SDS, so that the target soft material droplet (24) can stably suspend in water, the topological structure of the target soft material droplet (24) reaches a steady state is a topological line (26) uniformly distributed near the surface of the target soft material droplet, and the topological line (26) is connected end to form a circular ring;
in the third step, the aqueous solution is injected into the first target soft substance droplet (24) by the laser injection system to form a first sub-droplet (28), the first sub-droplet (28) is captured by the topological line (26) and moves along the space geometric form of the topological line, and the topological line (26) is completely occupied by the first sub-droplet (28) to form a particle necklace-shaped structure along with the laser injection.
3. A method of using an extreme manufacturing apparatus for three-dimensional sphere structures of microdroplets according to claim 1, characterized in that: in the step 1, the target droplet (7) is doped with 0.05wt% of R811, the target soft material droplet (30) is obtained when the doping concentration reaches a steady state, the topological structure is a topological point (31), and the aqueous solution in the step three is doped with 0.2wt% of surfactant SDS, so that the target soft material droplet (30) can be stably suspended in water;
in the third step, when the laser exposure is 5s, the aqueous solution is injected into the second target soft material droplet (30) to form a second sub-droplet (35), the second sub-droplet (35) is affected by the long-range elastic interaction force of the topological point (31), the first injected second sub-droplet (35) is captured by the topological point (31) and attracts the rest of the sub-droplets to form a droplet chain, meanwhile, the liquid crystal molecules in the second target soft material droplet (30) are arranged along the topological point (31) to be perpendicular to the sphere surface of the second target soft material droplet (30), spontaneous tissues of the second injected sub-droplet (35) form a droplet chain under the elastic action of the liquid crystal molecules, and the alignment direction of the droplet chain in the sphere space of the second target soft material droplet (30) is consistent with the alignment direction of the liquid crystal molecules, and along with the progress of laser injection, the injected second sub-droplet (35) forms a spiral micro-nanoparticle chain (34) along the sub-alignment direction in the second target soft material droplet (30).
4. A method of using an extreme manufacturing apparatus for three-dimensional sphere structures of microdroplets according to claim 3, characterized in that: the E7 liquid crystal is doped with a heat absorbing material.
CN202210202943.7A 2022-03-02 2022-03-02 Extreme manufacturing equipment of three-dimensional sphere structure of microdroplet and use method Active CN114797696B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202210202943.7A CN114797696B (en) 2022-03-02 2022-03-02 Extreme manufacturing equipment of three-dimensional sphere structure of microdroplet and use method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202210202943.7A CN114797696B (en) 2022-03-02 2022-03-02 Extreme manufacturing equipment of three-dimensional sphere structure of microdroplet and use method

Publications (2)

Publication Number Publication Date
CN114797696A CN114797696A (en) 2022-07-29
CN114797696B true CN114797696B (en) 2023-04-25

Family

ID=82529530

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202210202943.7A Active CN114797696B (en) 2022-03-02 2022-03-02 Extreme manufacturing equipment of three-dimensional sphere structure of microdroplet and use method

Country Status (1)

Country Link
CN (1) CN114797696B (en)

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH1078583A (en) * 1996-09-03 1998-03-24 Sekisui Chem Co Ltd Production of optical functional element
US20100204459A1 (en) * 2007-08-27 2010-08-12 The Regents Of The University Of California Systems and methods for producing multi-component colloidal structures
EP2691676B1 (en) * 2011-03-30 2019-03-27 Bio-Rad Laboratories, Inc. Injection of multiple volumes into or out of droplets
CN102435148A (en) * 2011-09-09 2012-05-02 上海理工大学 Laser feedback effect-based microscopic three-dimensional profile measurement system
EP3063529A2 (en) * 2013-10-30 2016-09-07 Premium Genetics (UK) Ltd. Microfluidic system and method with focused energy apparatus
CN108872084B (en) * 2018-04-24 2022-05-17 河北工业大学 All-optical micro-droplet separation method based on asymmetric lithium niobate interlayer structure

Also Published As

Publication number Publication date
CN114797696A (en) 2022-07-29

Similar Documents

Publication Publication Date Title
CN104324769B (en) Generation method based on the drop of microchannel
Le Gac et al. Sonoporation of suspension cells with a single cavitation bubble in a microfluidic confinement
CN106093443B (en) It is a kind of that lithium niobate chip microlayer model transportation method controllable in real time is cut based on C
CN109201130A (en) A kind of double emulsions capillary glass tube micro-fluidic chip and its manufactured phase-change microcapsule
WO2019144894A1 (en) Motion control mechanism, liquid spitting pipette tip, micro-droplet generating device, fluid driving mechanism and fluid driving method, micro-droplet generating method, and surface treatment method for liquid spitting pipette tip
CN105921355A (en) Real-time controllable micro-droplet jetting device and method based on lithium niobate crystal
CN114797696B (en) Extreme manufacturing equipment of three-dimensional sphere structure of microdroplet and use method
Huang et al. Coaxial oblique interface shearing: tunable generation and sorting of double emulsions for spatial gradient drug release
US7534598B2 (en) Apparatus and method for injecting substance into cell
Lin et al. Directional transport of underwater bubbles on solid substrates: principles and applications
Doppler et al. Co-flow injection for serial crystallography at X-ray free-electron lasers
CN113773521B (en) Method for preparing emulsion and polymer particles with size less than 10 nanometers based on droplet self-breaking phenomenon
CN111307714B (en) Droplet control chip based on optical flow control thermal capillary micro-flow vortex and control method thereof
CN108031499B (en) Light-induced micro-droplet continuous generation and transfer method
CN111391303B (en) Preparation method of three-dimensional super-smooth fabric based on microfluidic 3D printing technology
CN112452251B (en) Crescent-shaped and deformed ceramic microparticle, preparation method, application and preparation device thereof
CN211246358U (en) Ultrasonic auxiliary circulating type membrane dispersing device
CN111504598B (en) Experimental device for promoting liquid drop impact splashing and application thereof
CN114345429A (en) Double-emulsion drop directional rapid breaking and releasing method based on near-infrared thermal effect
CN105057013A (en) Method for producing three-dimensional connected micro-channel based on spatially selective induction of glass phase separation
JP5185527B2 (en) Gene transfer device
US20200190452A1 (en) Three-dimensional silicon scaffold for tissue engineering
CN114225977A (en) Multi-core multi-component micro-droplet processing system
CN113477283B (en) Method for driving fluid to move by non-plasma metal photoinduced ultrasound and capturing device
CN116059890B (en) Micro-hybrid robot device based on sound-dispelling bubble oscillation and hybrid control method

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant