CN114796302A - Extraction method of active ingredients of jatropha - Google Patents
Extraction method of active ingredients of jatropha Download PDFInfo
- Publication number
- CN114796302A CN114796302A CN202210385963.2A CN202210385963A CN114796302A CN 114796302 A CN114796302 A CN 114796302A CN 202210385963 A CN202210385963 A CN 202210385963A CN 114796302 A CN114796302 A CN 114796302A
- Authority
- CN
- China
- Prior art keywords
- acetic acid
- acid aqueous
- aqueous solution
- solution
- extracting
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/45—Ericaceae or Vacciniaceae (Heath or Blueberry family), e.g. blueberry, cranberry or bilberry
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention relates to the technical field of biochemistry, in particular to a method for extracting active ingredients of druvain, which comprises the steps of weighing druvain leaf powder and acetic acid aqueous solution, wherein the proportion range of the material-liquid ratio between the druvain leaf powder and the acetic acid aqueous solution is 0.025 g/mL-0.1 g/mL; pouring the weighed Lolium kava leaf powder and acetic acid aqueous solution into a round-bottom flask, and adding an enzyme catalyst, wherein the weight ratio of the enzyme catalyst to the Lolium kava leaf powder is 0.2-0.6%; stirring a mixed solution formed by the jatropha leaves powder, the acetic acid aqueous solution and the enzyme catalyst, controlling the temperature of the mixed solution to be 50 ℃, and controlling the reaction time of the mixed solution to be between 6 and 24 hours; after the mixed solution is stirred, filtering the mixed solution by using gauze to obtain a primary extract; filtering the primary extract with Buchner funnel to obtain the effective components of Piper methysticum. The method uses the semi-bionic extraction method to extract the effective components of the jaboticaba, and greatly improves the extraction efficiency of the jaboticamin compared with the traditional extraction method.
Description
Technical Field
The invention relates to the technical field of biochemistry, and particularly relates to a method for extracting an effective component of drupelagia.
Background
Piper hancei is a plant of Ericaceae, belonging to evergreen shrub or arbory, as high as 4 m, and is distributed in Anhui, Zhejiang, Fujian, etc. of China, and its leaves are toxic and can be used as pesticide.
The extraction method of the effective components of the traditional Chinese medicine of the Okava comprises a water extraction method, a methanol extraction method and an ethanol extraction method. The water extraction method has long extraction time and low extraction efficiency, while the methanol extraction method and the ethanol extraction method need to be accurately operated on a water bath, the accuracy is not easy to be controlled, and meanwhile, the methanol and the ethanol are easy to volatilize, so that a large amount of resources are wasted, and the methanol and the ethanol belong to flammable liquid and have high dangerousness.
In conclusion, the extraction method of the active ingredients of the drupelagia zizanoides in the prior art has the technical problems of low extraction efficiency and high extraction risk.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides a method for extracting the active ingredients of drupelagia, which solves the technical problems of low extraction efficiency and high extraction risk of the method for extracting the active ingredients of drupelagia in the prior art.
(II) technical scheme
In order to solve the technical problem, the invention provides a method for extracting effective components of drupelagia, which comprises the following steps: weighing Chinese jatropha leaf powder and an acetic acid aqueous solution, wherein the ratio of the Chinese jatropha leaf powder to the acetic acid aqueous solution is in the range of 0.025g/mL to 0.1 g/mL; pouring the weighed drupelagia leaf powder and the acetic acid aqueous solution into a round-bottom flask, and adding an enzyme catalyst, wherein the weight ratio of the enzyme catalyst to the drupelagia leaf powder is 0.2-0.6%; stirring a mixed solution formed by the jaboticaba leaf powder, the acetic acid aqueous solution and the enzyme catalyst, controlling the temperature of the mixed solution to be 50 ℃, and controlling the reaction time of the mixed solution to be between 6 and 24 hours; after the mixed solution is stirred, filtering the mixed solution by using gauze to obtain a primary extract; filtering the primary extract with Buchner funnel to obtain the effective components of Piper methysticum.
Further, the enzyme catalyst is one of the following: plant extraction enzyme, cellulase, alpha-amylase or pectinase.
Further, the enzyme catalyst is cellulase, and the method further comprises: the weight of the above Lolium kawachii leaf powder is 10g, the weight of the above cellulase is 20mg, the volume of the above acetic acid aqueous solution is 100mL, the pH value of the above acetic acid aqueous solution is 5, and the above reaction time is 12 h.
Further, the enzyme catalyst is an alpha-amylase, and the method further comprises: the weight of the above Lolium kawachii leaf powder is 10g, the weight of the above alpha-amylase is 20mg, the volume of the above acetic acid aqueous solution is 100mL, the pH value of the above acetic acid aqueous solution is 5, and the reaction time is 12 h.
Further, the enzyme catalyst is pectinase, and the method further comprises: the weight of the above Lolium kawachii leaf powder is 10g, the weight of the above pectinase is 20mg, the volume of the above acetic acid aqueous solution is 100mL, the pH of the above acetic acid aqueous solution is 5, and the reaction time is 12 h.
Further, the rotating speed value of the rotating stirring of the mixed solution is between 35r/min and 60 r/min.
Further, the pH value of the acetic acid aqueous solution ranges from 4.8 to 5.2.
(III) advantageous effects
The invention has the following beneficial effects:
the method uses the semi-bionic extraction method to extract the effective components of the drupelagia, compared with a water extraction method, the processing time is integrally shortened, and the extraction efficiency of the effective components of the drupelagia is greatly improved.
The method uses the semi-bionic extraction method to extract the effective components of the drupelia kadsura, has low cost of the whole raw materials compared with a methanol extraction method and an ethanol extraction method, is easy to carry out accurate quantitative operation, and is safer compared with the methanol extraction method and the ethanol extraction method.
Drawings
FIG. 1 is a schematic flow chart of the extraction method of the active ingredients of Piper methysticum in the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Fig. 1 is a schematic flow chart of a method for extracting effective components from kava, as shown in fig. 1, the method comprises:
s101, weighing the jatropha powder and an acetic acid aqueous solution, wherein the ratio of the jatropha powder to the acetic acid aqueous solution is 0.025g/mL to 0.1 g/mL;
s103, pouring the weighed Lolium kava leaf powder and an acetic acid aqueous solution into a round-bottom flask, and adding an enzyme catalyst, wherein the weight ratio of the enzyme catalyst to the Lolium kava leaf powder is 0.2-0.6%;
s105, stirring a mixed solution formed by the jaboticaba leaf powder, the acetic acid aqueous solution and the enzyme catalyst, controlling the temperature of the mixed solution to be 50 ℃, and controlling the reaction time of the mixed solution to be between 6 and 24 hours;
s107, after the mixed solution is stirred, filtering the mixed solution by using gauze to obtain a primary extract;
s109, filtering the primary extract by using a Buchner funnel to obtain the active ingredients of the drupelia kadsura.
The method uses the semi-bionic extraction method to extract the effective components of the drupelagia, compared with a water extraction method, the processing time is integrally shortened, and the extraction efficiency of the effective components of the drupelagia is greatly improved. The method uses the semi-bionic extraction method to extract the effective components of the drupelia kadsura, has low cost of the whole raw materials compared with a methanol extraction method and an ethanol extraction method, is easy to carry out accurate quantitative operation, and is safer compared with the methanol extraction method and the ethanol extraction method.
The semi-bionic extraction method combines the traditional method of oral administration of traditional Chinese medicines with the modern biological pharmacy theory, not only accords with the traditional theory and experience that the traditional Chinese medicine and pharmacy pay attention to the prescription to comprehensively exert the drug effect, but also is in line with the modern science and technology that the western medicine pays attention to the effective components of the monomers and uses animal test indexes to evaluate the drug effect and the safety, so the semi-bionic extraction method has obvious technical effect when being used for the effective components of the drumstick tree.
Further, the enzyme catalyst is one of the following: plant extraction enzyme, cellulase, alpha-amylase or pectinase.
Further, the enzyme catalyst is cellulase, and the method further comprises the following steps: the weight of the jatropha leaves powder is 10g, the weight of the cellulase is 20mg, the volume of the acetic acid aqueous solution is 100mL, the pH value of the acetic acid aqueous solution is 5, and the reaction time is 12 h.
Further, the enzyme catalyst is an alpha-amylase, and the method further comprises: the weight of the jatropha leaves powder is 10g, the weight of the alpha-amylase is 20mg, the volume of the acetic acid aqueous solution is 100mL, the pH value of the acetic acid aqueous solution is 5, and the reaction time is 12 h.
Further, the enzyme catalyst is pectinase, and the method further comprises: the weight of the jatropha leaves powder is 10g, the weight of the pectinase is 20mg, the volume of the acetic acid aqueous solution is 100mL, the pH value of the acetic acid aqueous solution is 5, and the reaction time is 12 h.
Further, the rotating speed value of the rotating stirring mixed solution ranges from 35r/min to 60 r/min.
Further, the pH value of the acetic acid aqueous solution ranges from 4.8 to 5.2.
The technical scheme of the invention also comprises the following multiple embodiments:
example one
A method for extracting effective components from Piper methysticum comprises adding powder of 10g Piper methysticum leaves, 20mg plant extraction enzyme, 100mL acetic acid water solution (pH 5), stirring at 50 deg.C for 12h, filtering with gauze, and further filtering with Buchner funnel to obtain clear brown extractive solution, which is effective components of Piper methysticum.
Example two
A method for extracting effective components from Piper methysticum comprises adding 10g of Piper methysticum leaf powder, 20mg of cellulase and 100mL of acetic acid water solution (pH 5) into round bottom flask, and stirring at 50 deg.C for reaction for 12 hr. After the reaction is finished, carrying out primary filtration on the reaction solution by using gauze, and then further filtering by using a Buchner funnel to obtain a clear tan extracting solution, wherein the tan extracting solution is the active ingredient of the jaboticaba.
EXAMPLE III
A method for extracting effective components from Piper methysticum comprises adding 10g of Piper methysticum leaf powder, 20mg of alpha-amylase, and 100mL of acetic acid water solution (pH 5) into round bottom flask, and stirring at 50 deg.C for 12 h. After the reaction is finished, carrying out primary filtration on the reaction solution by using gauze, and then further filtering by using a Buchner funnel to obtain a clear tan extracting solution, wherein the tan extracting solution is the active ingredient of the jaboticaba.
Example four
A method for extracting effective components from Piper methysticum comprises adding 10g of Piper methysticum leaf powder, 20mg of pectinase and 100mL of acetic acid aqueous solution (pH 5) into round-bottom flask, and reacting at 50 deg.C under stirring for 12 h. After the reaction is finished, carrying out primary filtration on the reaction solution by using gauze, and then further filtering by using a Buchner funnel to obtain a clear tan extracting solution, wherein the tan extracting solution is the active ingredient of the jaboticaba.
EXAMPLE five
A method for extracting effective components from Piper methysticum comprises adding 10g of Piper methysticum leaf powder, 60mg of one of plant extraction enzyme, cellulase, alpha-amylase, and pectinase, and 100mL of acetic acid water solution (pH 5, stirring at 50 deg.C, reacting for 12h, filtering with gauze, and further filtering with Buchner funnel to obtain clear brown extractive solution, which is effective components of Piper methysticum.
EXAMPLE six
A method for extracting effective components from Piper methysticum comprises adding 10g of Piper methysticum leaf powder, 40mg of plant extraction enzyme, and 100mL of acetic acid water solution (pH 4.8-5.2) into round bottom flask, and stirring at 50 deg.C for 12 hr. After the reaction is finished, carrying out primary filtration on the reaction solution by using gauze, and then further filtering by using a Buchner funnel to obtain a clear tan extracting solution, wherein the tan extracting solution is the active ingredient of the jaboticaba.
EXAMPLE seven
A method for extracting effective components from Piper methysticum comprises adding 10g of Piper methysticum leaf powder, 40mg of plant extraction enzyme, and 100mL of acetic acid water solution (pH 4.8-5.2) into round bottom flask, and stirring at 50 deg.C for 18 h. After the reaction is finished, carrying out primary filtration on the reaction solution by using gauze, and then further filtering by using a Buchner funnel to obtain a clear tan extracting solution, wherein the tan extracting solution is the active ingredient of the jaboticaba.
Example eight
A method for extracting effective components from Piper methysticum comprises adding 10g of Piper methysticum leaf powder, 20mg of plant extraction enzyme, and 100mL of acetic acid water solution (pH 5) into round bottom flask, and stirring at 50 deg.C for reaction for 6 h. After the reaction is finished, carrying out primary filtration on the reaction solution by using gauze, and then further filtering by using a Buchner funnel to obtain a clear tan extracting solution, wherein the tan extracting solution is the active ingredient of the jaboticaba.
Example nine
A method for extracting effective components from Piper methysticum comprises adding 10g of Piper methysticum leaf powder, 20mg of plant extraction enzyme, and 100mL of acetic acid water solution (pH 5) into round bottom flask, and stirring at 50 deg.C for 18 h. After the reaction is finished, carrying out primary filtration on the reaction solution by using gauze, and then further filtering by using a Buchner funnel to obtain a clear tan extracting solution, wherein the tan extracting solution is the active ingredient of the jaboticaba.
Example ten
A method for extracting effective components from Piper methysticum comprises adding 10g of Piper methysticum leaf powder, 20mg of plant extraction enzyme, and 100mL of acetic acid water solution (pH 5) into round bottom flask, and stirring at 50 deg.C for 24 hr. After the reaction is finished, carrying out primary filtration on the reaction solution by using gauze, and then further filtering by using a Buchner funnel to obtain a clear tan extracting solution, wherein the tan extracting solution is the active ingredient of the jaboticaba.
EXAMPLE eleven
A method for extracting effective components from Piper methysticum comprises adding 10g of Piper methysticum leaf powder, 40mg of plant extraction enzyme, and 100mL of acetic acid water solution (pH 5.2) into round bottom flask, and stirring at 50 deg.C for 12 h. After the reaction is finished, carrying out primary filtration on the reaction solution by using gauze, and then further filtering by using a Buchner funnel to obtain a clear tan extracting solution, wherein the tan extracting solution is the active ingredient of the jaboticaba.
Example twelve
A method for extracting effective components from Piper methysticum comprises adding 10g of Piper methysticum leaf powder, 40mg of plant extraction enzyme, and 200mL of acetic acid water solution (pH 5) into round bottom flask, and stirring at 50 deg.C for 12 h. After the reaction is finished, carrying out primary filtration on the reaction solution by using gauze, and then further filtering by using a Buchner funnel to obtain a clear tan extracting solution, wherein the tan extracting solution is the active ingredient of the jaboticaba.
EXAMPLE thirteen
A method for extracting effective components from Piper methysticum comprises adding 10g of Piper methysticum leaf powder, 40mg of plant extraction enzyme, and 300mL of acetic acid water solution (pH 5) into round bottom flask, and stirring at 50 deg.C for 12 h. After the reaction is finished, carrying out primary filtration on the reaction solution by using gauze, and then further filtering by using a Buchner funnel to obtain a clear tan extracting solution, wherein the tan extracting solution is the active ingredient of the jaboticaba.
Example fourteen
A method for extracting effective components from Piper methysticum comprises adding 10g of Piper methysticum leaf powder, 40mg of plant extraction enzyme, and 400mL of acetic acid water solution (pH 5) into round bottom flask, and stirring at 50 deg.C for 12 h. After the reaction is finished, carrying out primary filtration on the reaction solution by using gauze, and then further filtering by using a Buchner funnel to obtain a clear tan extracting solution, wherein the tan extracting solution is the active ingredient of the jaboticaba.
The high performance liquid chromatography experiment proves that the embodiment is feasible.
| Extraction method | Extraction time | Purity of the extract |
| The method of the present application | 6-24h | 78.7％ |
| Water extraction method | 12-36h | 72.4％ |
TABLE 1
As shown in table 1, the conventional water extraction method takes much time compared with the present application, and the present invention greatly improves the extraction efficiency.
The method uses the semi-bionic extraction method to extract the effective components of the drupelagia, compared with a water extraction method, the processing time is integrally shortened, and the extraction efficiency of the effective components of the drupelagia is greatly improved. The method uses the semi-bionic extraction method to extract the effective components of the drupelia kadsura, has low cost of the whole raw materials compared with a methanol extraction method and an ethanol extraction method, is easy to carry out accurate quantitative operation, and is safer compared with the methanol extraction method and the ethanol extraction method.
While there have been shown and described what are at present considered the fundamental principles and essential features of the invention and its advantages, it will be apparent to those skilled in the art that the invention is not limited to the details of the foregoing exemplary embodiments, but is capable of other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (7)
1. A method for extracting active ingredients of drupelagia is characterized by comprising the following steps:
weighing Chinese jatropha leaf powder and an acetic acid aqueous solution, wherein the ratio of the Chinese jatropha leaf powder to the acetic acid aqueous solution is in the range of 0.025g/mL to 0.1 g/mL;
pouring the weighed drupelagia leaf powder and the acetic acid aqueous solution into a round-bottom flask, and adding an enzyme catalyst, wherein the weight ratio of the enzyme catalyst to the drupelagia leaf powder is 0.2-0.6%;
stirring a mixed solution formed by the jatropha leaves powder, the acetic acid aqueous solution and the enzyme catalyst, controlling the temperature of the mixed solution to be 50 ℃, and controlling the reaction time of the mixed solution to be between 6 and 24 hours;
after the mixed solution is stirred, filtering the mixed solution by using gauze to obtain a primary extract;
filtering the primary extract with Buchner funnel to obtain the effective components of Piper methysticum.
2. The method for extracting the active ingredients of drumstick tree according to claim 1, wherein the enzyme catalyst is one of the following: plant extraction enzyme, cellulase, alpha-amylase or pectinase.
3. The method for extracting the active ingredients of drupelia kawai as claimed in claim 2, wherein the enzyme catalyst is cellulase, and the method further comprises: the weight of the drupelia leaves powder is 10g, the weight of the cellulase is 20mg, the volume of the acetic acid aqueous solution is 100mL, the pH value of the acetic acid aqueous solution is 5, and the reaction time is 12 h.
4. The method for extracting the active ingredients of kava according to claim 2, wherein the enzyme catalyst is alpha-amylase, and the method further comprises: the weight of the jatropha leaves powder is 10g, the weight of the alpha-amylase is 20mg, the volume of the acetic acid aqueous solution is 100mL, the pH value of the acetic acid aqueous solution is 5, and the reaction time is 12 h.
5. The method for extracting the active ingredients of kava according to claim 2, wherein the enzyme catalyst is pectinase, and the method further comprises: the weight of the jatropha leaves powder is 10g, the weight of the pectinase is 20mg, the volume of the acetic acid aqueous solution is 100mL, the pH value of the acetic acid aqueous solution is 5, and the reaction time is 12 h.
6. The method of claim 1, wherein the rotational speed of the rotational agitation of the mixed solution is in a range of 35r/min to 60 r/min.
7. The method for extracting the active ingredients of kava according to claim 1, wherein the pH of the aqueous acetic acid solution is in the range of 4.8 to 5.2.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210385963.2A CN114796302A (en) | 2022-04-13 | 2022-04-13 | Extraction method of active ingredients of jatropha |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210385963.2A CN114796302A (en) | 2022-04-13 | 2022-04-13 | Extraction method of active ingredients of jatropha |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114796302A true CN114796302A (en) | 2022-07-29 |
Family
ID=82536268
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210385963.2A Pending CN114796302A (en) | 2022-04-13 | 2022-04-13 | Extraction method of active ingredients of jatropha |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114796302A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101130887A (en) * | 2007-09-27 | 2008-02-27 | 江苏紫荆花纺织科技股份有限公司 | Method for degumming jute by using complex enzyme |
| CN102060696A (en) * | 2010-12-20 | 2011-05-18 | 昆明理工大学 | 9,10-open-ring multi-acylation leucothoe alkane diterpenoid compounds and preparation method thereof |
| CN107011179A (en) * | 2017-03-20 | 2017-08-04 | 陕西金冠牧业有限公司 | A kind of extraction separation method of Achnatherum inebrians toxin |
-
2022
- 2022-04-13 CN CN202210385963.2A patent/CN114796302A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101130887A (en) * | 2007-09-27 | 2008-02-27 | 江苏紫荆花纺织科技股份有限公司 | Method for degumming jute by using complex enzyme |
| CN102060696A (en) * | 2010-12-20 | 2011-05-18 | 昆明理工大学 | 9,10-open-ring multi-acylation leucothoe alkane diterpenoid compounds and preparation method thereof |
| CN107011179A (en) * | 2017-03-20 | 2017-08-04 | 陕西金冠牧业有限公司 | A kind of extraction separation method of Achnatherum inebrians toxin |
Non-Patent Citations (1)
| Title |
|---|
| 姚广民等: "马醉木Pieris japonica叶中的三萜类化学成分" * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR102229831B1 (en) | A Composition for extracting effective ingredients from plant comprising deep eutectic solvent and a method for extracting effective ingredients from plant using the same | |
| Pepato et al. | Cissus sicyoides (princess vine) in the long‐term treatment of streptozotocin‐diabetic rats | |
| CN108392500B (en) | Method for preparing ganoderma triterpene | |
| CN109157454A (en) | A kind of Camellia combined extracts and its preparation and the application in cosmetics | |
| CN101181354A (en) | Fructus schizandrae chemical composition group extract and preparation technique | |
| CN114796302A (en) | Extraction method of active ingredients of jatropha | |
| CN102550828A (en) | Fattening and weighting agent for beefs | |
| CN111937748B (en) | Culture method for improving content of ginsenoside Rg1 and Re in ginseng adventitious roots | |
| CN111758854A (en) | Feed additive and chicken feed containing same | |
| CN101550125B (en) | Hazelnut element B, extracting method and pharmaceutical use thereof | |
| CN109771469A (en) | A kind of vinegar toast root of gansui and its concocting method | |
| CN105106267A (en) | Common sowthistle herb tannin extracting process | |
| CN112156123B (en) | Viburnum edulis extract and preparation method and application thereof | |
| CN111440186B (en) | Coumarin norisoflavone compound extracted from small horse blebs and having liver protecting effect, and preparation method and application thereof | |
| CN108836994B (en) | Method for extracting saponins active ingredients from bark of artificial forest | |
| CN102838882A (en) | Extraction method of chestnut shell coloring matters | |
| CN112724115A (en) | Firecracker spark cream and preparation method thereof | |
| CN101633616B (en) | Preparation method and medicinal application of tanshinol | |
| CN110075154A (en) | A method of saponin(e active component is extracted from black wattle bark based on depth congruent melting solvent | |
| CN108997280A (en) | A kind of benzofuran type compound and preparation method thereof and treatment heart disease purposes | |
| CN113041309A (en) | Green extraction solvent for natural antioxidant in betel nut seeds, preparation method and application | |
| Baek et al. | Comparative Study of Bioactivity of Leaves and Stems of Pleurospermum camtschaticum Hoffm | |
| RU2465000C1 (en) | Method for preparing agent showing cholagogue action | |
| CN108794438A (en) | A kind of benzofuran type compound and preparation method thereof and treat cardiopathic purposes | |
| CN106867682A (en) | A kind of method that biodiesel is prepared with idesia polycarpa oil |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |