CN114796287A - Probiotic powder capable of preventing decayed teeth and refreshing oral cavity and application thereof - Google Patents
Probiotic powder capable of preventing decayed teeth and refreshing oral cavity and application thereof Download PDFInfo
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- CN114796287A CN114796287A CN202210648958.6A CN202210648958A CN114796287A CN 114796287 A CN114796287 A CN 114796287A CN 202210648958 A CN202210648958 A CN 202210648958A CN 114796287 A CN114796287 A CN 114796287A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
- A61K35/747—Lactobacilli, e.g. L. acidophilus or L. brevis
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/702—Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
- A61K35/745—Bifidobacteria
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/26—Carbohydrates, e.g. sugar alcohols, amino sugars, nucleic acids, mono-, di- or oligo-saccharides; Derivatives thereof, e.g. polysorbates, sorbitan fatty acid esters or glycyrrhizin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/19—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles lyophilised, i.e. freeze-dried, solutions or dispersions
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/02—Stomatological preparations, e.g. drugs for caries, aphtae, periodontitis
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
Abstract
The invention discloses probiotic powder capable of preventing decayed teeth and refreshing oral cavity and application thereof, belonging to the technical field of microorganisms and the technical field of medicines. The probiotic powder disclosed by the invention consists of lactobacillus gasseri HHuMin D, lactobacillus fermentum OK, bifidobacterium bifidum BGN4, bifidobacterium lactis AD011, sugar alcohol sweetening agents and prebiotics, can improve oral problems, and is specifically embodied in that: the probiotic powder can inhibit the growth of oral bacteria and improve fresh oral smell; inhibiting the formation of streptococcus mutans, and preventing dental caries; it can effectively bind with toxin and Streptococcus sanguis to inhibit formation of dental plaque. Therefore, the probiotic powder containing lactobacillus gasseri HHuMin D, lactobacillus fermentum OK, bifidobacterium bifidum BGN4 and bifidobacterium lactis AD011 has great application prospect in improving oral problems.
Description
Technical Field
The invention relates to probiotic powder capable of preventing decayed teeth and refreshing oral cavity and application thereof, belonging to the technical field of microorganisms and the technical field of medicines.
Background
In the oral cavity of a human body, there are over 700 kinds of microorganisms, and because of the environments such as moisture, pH, nutrients, etc. suitable for the propagation of the various microorganisms, it is very desirable that there are a variety of indigenous bacteria colonies in various environments such as saliva, tooth surface dental plaque, tongue back, mucous membrane of both cheeks and gingival crevice.
In the oral cavity, as in our intestinal tract, it is important that the beneficial and harmful bacteria remain in a balanced steady state. Imbalance of oral flora can cause various oral diseases. Therefore, to fundamentally solve the oral problems, it is essential to maintain a balanced steady state between beneficial bacteria and harmful bacteria for a long period of time by using the indigenous flora in the oral cavity.
Oral health and human health are closely related. The data reported show that people with oral diseases have a 3.4 times higher probability of having systemic disease than those with healthy mouth; and harmful bacteria which easily cause periodontal inflammation and peri-implant periodontal inflammation in the oral cavity easily permeate to important organs of the whole body through blood and cause secondary infection. Harmful bacteria permeate into blood to cause adverse effects on various parts of the body, and the disease condition is worsened as metabolites of the harmful bacteria permeate into the whole body. In addition, various harmful bacteria reach the intestinal tract, which also causes intestinal flora disorder and various diseases. In daily life, when people have oral diseases, most of people adopt an artificial method, a physical mechanical method and a medicinal chemical method to relieve pain, and most of people choose to hide peculiar smell of oral cavity by invisible or using a breath freshener.
Disclosure of Invention
Aims to solve the problems of oral diseases such as periodontitis, decayed tooth, dental plaque, halitosis and the like caused by the proliferation of pathogenic bacteria or the problem of bad breath odor caused by dyspepsia. According to the discovery results of the existing clinic and researchers, the probiotic powder capable of preventing decayed teeth and refreshing the oral cavity and the application thereof are provided, and the probiotic powder comprises the following components:
the invention provides probiotic powder capable of preventing decayed teeth and refreshing oral cavity, which consists of lactobacillus gasseri HHuMin D, lactobacillus fermentum OK, bifidobacterium bifidum BGN4, bifidobacterium lactis AD011, sugar alcohol sweetening agent and prebiotics; the lactobacillus gasseri HHuMin D, the lactobacillus fermentum OK, the bifidobacterium bifidum BGN4 and the bifidobacterium lactis AD011 are main adjusting components, and the prebiotics and the sugar alcohol sweetening agent are auxiliary adjusting components; wherein: the prebiotics comprise galacto-oligosaccharide, fructo-oligosaccharide and xylo-oligosaccharide, and the sugar alcohol sweetener comprises erythritol and xylitol.
In one embodiment of the present invention, the amount of each component of the probiotic powder added is as follows, in terms of 2g per bag:
lactobacillus gasseri HHuMin D50 hundred million CFU/bag (counting according to viable count)
Lactobacillus fermentum OK 50 hundred million CFU/bag (in terms of viable count)
Bifidobacterium bifidum BGN 450 hundred million CFU/bag (counting live bacteria)
Bifidobacterium lactis AD 01150 hundred million CFU/bag (in terms of viable count)
Erythritol 100 mg/bag
Xylitol 100 mg/bag
Galacto-oligosaccharide 48 mg/bag
108 mg/bag of fructo-oligosaccharide
Xylo-oligosaccharide 16 mg/bag
In an embodiment of the present invention, the preparation method of the lyophilized powder of probiotic bacteria includes the following steps:
1) respectively streaking Lactobacillus gasseri HHuMin D, Lactobacillus fermentum OK, Bifidobacterium bifidum BGN4 and Bifidobacterium lactis AD011 on MRS solid culture medium, and culturing at 37 deg.C for 48h to obtain single colony.
2) And selecting single colonies, respectively inoculating the single colonies in an MRS liquid culture medium, and culturing for 24-48 h at 37 ℃ for activation to obtain a first-level seed solution.
3) And respectively transferring the primary seed liquid into a seed culture medium according to the inoculation amount of 3% (v/v) to obtain a secondary seed liquid, and culturing for 24-48 h under the culture condition of 37 ℃.
4) And respectively inoculating the secondary seed liquid into a fermentation culture medium for high-density fermentation, wherein the inoculation amount is 3% (v/v), the culture condition is 35-37 ℃, and the culture is carried out for 24-48 h.
5) When the density of the thallus in the fermentation liquid reaches 10 8 ～10 9 CFU/mL, respectively collecting fermentation liquor; and respectively centrifuging the fermentation liquor to collect thalli.
6) And after the thalli are collected, respectively using a freeze-drying protective agent to carry out heavy suspension to obtain heavy suspensions.
7) And freeze-drying the heavy suspension by adopting a vacuum freezing method to obtain freeze-dried powder of lactobacillus gasseri HHuMin D, lactobacillus fermentum OK, bifidobacterium bifidum BGN4 and bifidobacterium lactis AD 011.
In one embodiment of the invention, the medium is MRS medium: 10g of peptone, 10g of beef extract powder, 20g of glucose, 2g of sodium acetate, 5g of yeast extract powder, 2g of diammonium hydrogen citrate and K 2 PO 4 ·3H 2 O 0.4g、MgSO 4 ·7H 2 O 0.2g、MnSO 4 0.05g, Tween 801 mL and agar 15g, and adjusting the pH value to 6.2-6.3.
In one embodiment of the invention, the lyoprotectant is prepared from the following components in percentage by weight: 15% of skimmed milk powder, 6% of trehalose, 4% of glycerol and 1.5% of xylitol.
In an embodiment of the present invention, the preparation method of the probiotic powder comprises the following steps:
1) mixing Lactobacillus gasseri HHuMin D50 hundred million CFU, Lactobacillus fermentum OK 50 hundred million CFU, Bifidobacterium bifidum BGN 450 hundred million CFU and Bifidobacterium lactis AD011 lyophilized powder 50 hundred million CFU.
2) Sequentially adding 100mg of erythritol, 100mg of xylitol, 48mg of galacto-oligosaccharide, 108mg of fructo-oligosaccharide and 16mg of xylo-oligosaccharide into the probiotic freeze-dried powder mixture, uniformly stirring, fully mixing, and subpackaging into bags to obtain the probiotic powder, wherein the bags are made of food-grade PE packaging materials with good sealability and safety, and the specification is 2 g.
In an embodiment of the present invention, the probiotic powder is used in an amount of:
the eating proposal for people of all ages (including all people after long teeth) is to drink with water with the temperature lower than 37 ℃ after meal, after tooth brushing and before sleeping or directly eat.
For severe oral diseases, the food is taken for 1 month in 2 bags per day, and after improvement, the food is taken for two months in 1 bag per day.
For the patients with normal oral health, the food is taken for 3 months in 1 bag per day.
If the antibiotics are taken, the antibiotics are recommended to be taken firstly, and then the probiotic powder is taken at an interval of 2-3 hours.
The invention also provides application of the probiotic powder capable of preventing decayed teeth and refreshing the oral cavity in improving the oral cavity problems caused by pathogenic bacteria proliferation and the oral odor problems caused by dyspepsia.
The invention has the beneficial effects that:
1) the invention provides probiotic powder capable of preventing decayed teeth and refreshing oral cavity, which consists of lactobacillus gasseri HHuMin D, lactobacillus fermentum OK, bifidobacterium bifidum BGN4, bifidobacterium lactis AD011, sugar alcohol sweetening agent and prebiotics. The probiotic powder can improve the oral problems caused by pathogenic bacteria proliferation and the bad breath odor caused by dyspepsia, and is specifically embodied in that: the probiotic powder can inhibit the growth of oral bacteria and improve fresh oral smell; inhibiting the formation of streptococcus mutans, and preventing dental caries; it can effectively bind with toxin and Streptococcus sanguis to inhibit formation of dental plaque. Therefore, the probiotic powder containing lactobacillus gasseri HHuMin D, lactobacillus fermentum OK, bifidobacterium bifidum BGN4 and bifidobacterium lactis AD011 has great application prospect in improving oral cavity problems caused by pathogenic bacteria proliferation and mouth odor problems caused by dyspepsia.
2) The lactobacillus gasseri HHuMin D and the lactobacillus fermentum OK are separated from saliva of healthy people; bifidobacterium bifidum BGN4 and Bifidobacterium lactis AD011 were isolated from the feces of healthy breast-fed infants. The sugar alcohol sweetening agent and the prebiotics are added in a matching way, and the allowable adding amount of the sugar alcohol sweetening agent and the prebiotics in the invention is in accordance with the allowable adding amount of GB/T29602-2014 and GB 2760-2014. Therefore, the probiotic powder is healthy and has no toxic or side effect on human bodies.
Detailed Description
The invention provides probiotic powder capable of preventing decayed teeth and refreshing oral cavity, which consists of lactobacillus gasseri HHuMin D, lactobacillus fermentum OK, bifidobacterium bifidum BGN4, bifidobacterium lactis AD011, sugar alcohol sweetening agent and prebiotics. The lactobacillus gasseri HHuMin D, the lactobacillus fermentum OK, the bifidobacterium bifidum BGN4 and the bifidobacterium lactis AD011 are main adjusting components, and the prebiotics and the sugar alcohol sweetening agent are auxiliary adjusting components; wherein: the prebiotics comprise galacto-oligosaccharide, fructo-oligosaccharide and xylo-oligosaccharide, and the sugar alcohol sweetener comprises erythritol and xylitol.
The following further describes the embodiments of the present invention. It should be noted that the description of the embodiments is provided to help understanding of the present invention, but the present invention is not limited thereto. In addition, the technical features related to the embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other.
The media involved in the following examples are as follows:
MRS culture medium: 10g of peptone, 10g of beef extract powder, 20g of glucose, 2g of sodium acetate, 5g of yeast extract powder, 2g of diammonium hydrogen citrate and K 2 PO 4 ·3H 2 O 0.4g、MgSO 4 ·7H 2 O 0.2g、MnSO 4 0.05g, 801 mL of Tween and 15g of agar, and adjusting the pH value to 6.2-6.3.
Example 1: preparation of bifidobacterium bifidum BGN4 freeze-dried powder
1) Bifidobacterium bifidum BGN4 was streaked on MRS solid medium and cultured at 37 ℃ for 48 hours to obtain single colonies.
2) And selecting a single colony, inoculating the single colony in an MRS liquid culture medium, and culturing for 24-48 h at 37 ℃ for activation to obtain a first-level seed solution.
3) And respectively transferring the primary seed liquid into a seed culture medium according to the inoculation amount of 3% (v/v) to obtain a secondary seed liquid, and culturing for 24-48 h under the culture condition of 37 ℃.
4) And respectively inoculating the secondary seed liquid into a fermentation culture medium for high-density fermentation, wherein the inoculation amount is 3% (v/v), the culture condition is 35-37 ℃, and the culture is carried out for 24-48 h.
5) When the density of the thallus in the fermentation liquid reaches 10 8 ～10 9 CFU/mL, respectively collecting fermentation liquor; and respectively centrifuging the fermentation liquor to collect thalli.
6) And (3) collecting the thallus, and then re-suspending the thallus by using a freeze-drying protective agent (the freeze-drying protective agent is prepared from the following components in percentage by weight: skimmed milk powder 15%, trehalose 6%, glycerol 4% and xylitol 1.5%) to obtain a resuspension.
7) And (3) freeze-drying the heavy suspension by adopting a vacuum freezing method to obtain bifidobacterium bifidum BGN4 freeze-dried powder.
EXAMPLE 2 preparation of freeze-dried powder of Lactobacillus gasseri HHuMin D
1) Lactobacillus gasseri HHuMin D is streaked on MRS solid culture medium and cultured for 48h at 37 ℃ to obtain single colony.
2) And selecting a single colony, inoculating the single colony in an MRS liquid culture medium, and culturing for 24-48 h at 37 ℃ for activation to obtain a first-level seed solution.
3) And respectively transferring the primary seed liquid into a seed culture medium according to the inoculation amount of 3% (v/v) to obtain a secondary seed liquid, and culturing for 24-48 h under the culture condition of 37 ℃.
4) And respectively inoculating the secondary seed liquid into a fermentation culture medium for high-density fermentation, wherein the inoculation amount is 3% (v/v), the culture condition is 35-37 ℃, and the culture is carried out for 24-48 h.
5) When the density of the thallus in the fermentation liquid reaches 10 8 ～10 9 CFU/mL, respectively collecting fermentation liquor; and respectively centrifuging the fermentation liquor to collect thalli.
6) And (3) collecting the thallus, and then re-suspending the thallus by using a freeze-drying protective agent (the freeze-drying protective agent is prepared from the following components in percentage by weight: skimmed milk powder 15%, trehalose 6%, glycerol 4% and xylitol 1.5%) to obtain a resuspension.
7) And (3) freeze-drying the heavy suspension by adopting a vacuum freezing method to obtain freeze-dried powder of lactobacillus gasseri HHuMin D.
Example 3 preparation of lyophilized powder of Bifidobacterium lactis AD011
1) The bifidobacterium lactis AD011 is streaked on an MRS solid culture medium and cultured for 48 hours at 37 ℃ to obtain a single colony.
2) And selecting a single colony, inoculating the single colony in an MRS liquid culture medium, and culturing for 24-48 h at 37 ℃ for activation to obtain a first-level seed solution.
3) And respectively transferring the primary seed liquid into a seed culture medium according to the inoculation amount of 3% (v/v) to obtain a secondary seed liquid, and culturing for 24-48 h under the culture condition of 37 ℃.
4) And respectively inoculating the secondary seed liquid into a fermentation culture medium for high-density fermentation, wherein the inoculation amount is 3% (v/v), the culture condition is 35-37 ℃, and the culture is carried out for 24-48 h.
5) When the density of the thallus in the fermentation liquid reaches 10 8 ～10 9 CFU/mL, respectively collecting fermentation liquor; and respectively centrifuging the fermentation liquor to collect thalli.
6) And (3) collecting the thallus, and then re-suspending the thallus by using a freeze-drying protective agent (the freeze-drying protective agent is prepared from the following components in percentage by weight: skimmed milk powder 15%, trehalose 6%, glycerol 4% and xylitol 1.5%) to obtain a resuspension.
7) And (3) freeze-drying the heavy suspension by adopting a vacuum freezing method to obtain freeze-dried powder of the bifidobacterium lactis AD 011.
Example 4 preparation of Lactobacillus fermentum OK lyophilized powder
1) Lactobacillus fermentum is streaked on MRS solid medium with OK, and cultured for 48h at 37 deg.C to obtain single colony.
2) And selecting a single colony, inoculating the single colony in an MRS liquid culture medium, and culturing for 24-48 h at 37 ℃ for activation to obtain a first-level seed solution.
3) And respectively transferring the primary seed liquid into a seed culture medium according to the inoculation amount of 3% (v/v) to obtain a secondary seed liquid, and culturing for 24-48 h under the culture condition of 37 ℃.
4) And respectively inoculating the secondary seed liquid into a fermentation culture medium for high-density fermentation, wherein the inoculation amount is 3% (v/v), the culture condition is 35-37 ℃, and the culture is carried out for 24-48 h.
5) When the density of the thallus in the fermentation liquid reaches 10 8 ～10 9 CFU/mL, respectively collecting fermentation liquor; and respectively centrifuging the fermentation liquor to collect thalli.
6) And (3) collecting the thallus, and then re-suspending the thallus by using a freeze-drying protective agent (the freeze-drying protective agent is prepared from the following components in percentage by weight: skimmed milk powder 15%, trehalose 6%, glycerol 4% and xylitol 1.5%) to obtain a resuspension.
7) And (4) freeze-drying the heavy suspension by adopting a vacuum freezing method to obtain lactobacillus fermentum OK freeze-dried powder.
Example 5 preparation of probiotic powder
The addition amount of each component of the probiotic powder is as follows according to the addition amount of 2g per bag:
lactobacillus gasseri HHuMin D50 hundred million CFU/bag (counting according to viable count)
Lactobacillus fermentum OK 50 hundred million CFU/bag (in terms of viable count)
Bifidobacterium bifidum BGN 450 hundred million CFU/bag (counting live bacteria)
Bifidobacterium lactis AD 01150 hundred million CFU/bag (in terms of viable count)
Erythritol 100 mg/bag
Xylitol 100 mg/bag
Galacto-oligosaccharide 48 mg/bag
108 mg/bag of fructo-oligosaccharide
Xylo-oligosaccharide 16 mg/bag
The preparation method of the probiotic powder comprises the following steps:
1) mixing Lactobacillus gasseri HHuMin D50 hundred million CFU, Lactobacillus fermentum OK 50 hundred million CFU, Bifidobacterium bifidum BGN 450 hundred million CFU and Bifidobacterium lactis AD 01150 hundred million CFU.
2) Sequentially adding 100mg of erythritol, 100mg of xylitol, 48mg of galacto-oligosaccharide, 108mg of fructo-oligosaccharide and 16mg of xylo-oligosaccharide into the probiotic freeze-dried powder mixture, uniformly stirring, fully mixing, and subpackaging into bags to obtain the probiotic powder, wherein the bags are made of food-grade PE packaging materials with good sealability and safety, and the specification is 2 g.
EXAMPLE 6 safety test
1. The safety of Lactobacillus gasseri HHuMin D, Lactobacillus fermentum OK, Bifidobacterium bifidum BGN4 and Bifidobacterium lactis AD011 is evaluated by evaluating hemolysis, mucin degradation, ammonia production, biogenic amine production, antimicrobial susceptibility analysis, transferability of antibiotic resistance, PCR data of antibiotic resistance genes, genome stability and whether virulence factors/virulence genes exist in blood cells.
2. Test results
1) None of the four strains showed hemolytic activity nor mucin-degrading activity, and no ammonia production was found.
2) The four strains do not produce 5-pentanediamine, histamine, tyramine, and produce small amounts of putrescine, similar to or less than other foods (e.g., spinach, tomato sauce, green beans, sauerkraut, and sausage).
3) The drug resistance (sensitivity) of the four strains to gentamicin and tetracycline is higher than the critical value of European Food Safety Agency (EFSA).
4) In the PCR analysis study, no resistance gene was found in the strain.
5) The resistance of the four strains to antibiotics was not transferred by binding to the gene fragment. .
6) The entire genomic sequence of the four strains has been published in GenBank.
7) The four strains do not contain known toxin-producing genes and pathogenic genes.
Example 7 safety testing of probiotic powder
1. Detection basis and method
Safety detection is carried out on the probiotic powder according to standard methods such as pollutant limit GB 2762-2017 in food safety national standard food, food microbiological inspection (total colony count determination GB 4789.2-2016, Escherichia coli count GB 4789.3-2016, Salmonella inspection GB 4789.4-2016 and mold inspection GB 4789.15-2016).
2. The result of the detection
The probiotic powder capable of preventing decayed teeth and refreshing the oral cavity is prepared by adopting a freeze-dried microcapsule three-layer embedding technology, and has the color of beige powder, no visible foreign matters in normal vision, no agglomeration, no peculiar smell and no odor. And the detection results of the pollutants of lead and tin are inactive, and the total number of bacterial colonies, coliform bacteria, salmonella and mould detection results are all passed.
EXAMPLE 8 concrete cases
Case 1: patient female, 32 years old, appealed: bad self-digestion, frequent bitter taste and bad smell. Eating the probiotic powder prepared by the invention, one bag is eaten within half an hour after meals, and the probiotic powder is rinsed with warm water for 10 seconds and swallowed, 2 times a day and persisted for 3 months. During the period, the oral cavity odor is improved and the breath becomes fresh by one return visit at an interval of 1 month.
Case 2: patient female, 26 years old, appealed: when the pressure is high, the patient will catch fire, and the gum will occasionally become inflamed and painful. Eating the probiotic powder prepared by the invention, one bag is eaten within half an hour after meals, the mouth is rinsed with warm water for 10 seconds, and the probiotic powder is swallowed 2 times a day and is insisted on eating for 1 month. Then, the food is continuously eaten for 6 months, and the return visit is carried out at intervals of 1 month, when the pressure is exerted on the internal heat, the gingival inflammation does not occur any more, and the internal heat and the peculiar smell in the oral cavity are improved to some extent.
Case 3: male patient, age 35, appealed: the alcohol administration often causes irregular diet and bad breath caused by dyspepsia. Eating the probiotic powder prepared by the invention, one bag is eaten within half an hour after meals, and the bag is rinsed with warm water for 10 seconds and swallowed, 2 times a day and kept for 15 days. The problem of bad breath is improved after 2 months of follow-up visit, and the method is repeatedly used when needed.
Case 4: patient girl, 6 years old, appeal: after the teeth are replaced, some teeth have black spots, namely decayed teeth, and the teeth often have sour odor in the mouth. Eating the probiotic powder prepared by the invention, one bag is eaten within half an hour after meals, and the probiotic powder is rinsed with warm water for 10 seconds and swallowed, 2 times a day and kept for 1 month. After 6 months of follow-up, the black spots on the teeth were not enlarged and spread, and the acid odor in the mouth was reduced to normal breath.
Case 5: male patient, 10 years old, appealed: yellow, rough and visible dirt adheres to the surfaces of teeth, halitosis is unpleasant, bleeding gums and swelling gums are often accompanied, and the teeth are diagnosed as being careless for cleaning 'dental plaque' accumulated for a long time. Eating the probiotic powder prepared by the invention, one bag is eaten within half an hour after meals, warm water is gargled for 10 seconds and spitted out, 2 times a day and lasts for 2 months, and patients are informed to remove tartar on the surface in time. After 6 months of follow-up visit, no obvious attachments are left on the surfaces of the teeth, bad breath smell is reduced, and the frequency of bleeding and swelling problems of the gum is reduced.
Example 9 clinical trial
1. Study object
34 patients with oral symptoms of bad breath (bitter taste and halitosis), small black spots on teeth (decayed teeth) and gingival inflammation in the age of 8-20 years old are selected. 17 patients were assigned to the treatment group and the remaining 17 patients were assigned to the placebo group.
2. Method of treatment
The treatment group was 2 bags of probiotic powder prepared according to the invention and the placebo group was an equivalent amount of skimmed milk powder without probiotics (placebo bag) visually indistinguishable from the probiotic bag. Each patient should eat the food within 30 minutes after meals, twice a day, 1 bag per time, and 20 days in total. The change of oral malodor, the area of small black spots on the teeth, and the number of times of gum inflammation before and after the treatment period were recorded.
3. Effect judgment standard
The effect is judged according to the change of the bad breath before and after the treatment period, the area of small black spots on the teeth and the inflammation times of the gum. The method has the following advantages: healing or better transformation of 3 problems than before treatment; and (4) invalidation: 3 problems before and after treatment continuously occur without obvious improvement trend and change.
4. Statistical method
Paired t-tests were performed to assess quantitative changes in oral symptoms in both groups: oral malodour changes before and after the study period, the area of small black spots on teeth, and the number of times of gum inflammation. When the p-value was <0.05, the results were considered statistically significant.
5. Test results
The results of 2 groups of effect evaluations are shown in Table 1.
TABLE 12 evaluation of the effects
As can be seen from table 1, the use of the probiotic powder of the present invention has a significant effect in improving the suppression of oral symptoms.
The invention provides a probiotic powder capable of preventing decayed teeth and refreshing oral cavity and application thereof, the probiotic powder comprises lactobacillus gasseri HHuMin D, lactobacillus fermentum OK, bifidobacterium bifidum BGN4, bifidobacterium lactis AD011, sugar alcohol sweetening agent and prebiotics, can improve the oral cavity problem caused by pathogenic bacteria proliferation and the halitosis problem caused by dyspepsia, and is specifically represented as follows: the probiotic powder can inhibit the growth of oral bacteria and improve fresh oral smell; inhibiting Streptococcus mutans formation, and preventing dental caries; it can effectively bind with toxin and Streptococcus sanguis to inhibit formation of dental plaque. Wherein, the application directions of the lactobacillus gasseri HHuMin D and the lactobacillus fermentum OK are verified in the aspects of inhibiting the growth of oral bacteria, improving fresh oral smell, preventing the occurrence of dental caries and inhibiting the formation of dental plaque. Bifidobacterium bifidum BGN4 and Bifidobacterium lactis AD011 reduce the generation of oral odor by regulating the problems of food retention in intestinal tract, dyspepsia, etc. Therefore, the probiotic powder containing Lactobacillus gasseri HHuMin D, Lactobacillus fermentum OK, Bifidobacterium bifidum BGN4 and Bifidobacterium lactis AD011 has great application prospect in improving oral cavity problems caused by pathogenic bacteria proliferation and halitosis problems caused by dyspepsia.
The foregoing is merely illustrative of the technical concepts and features of the present invention, which are intended to be known and practiced by those of ordinary skill in the art, and the scope of the present application is not limited by these specific embodiments. Various modifications and changes may occur to those skilled in the art. Any modification, equivalent change, equivalent replacement, improvement and the like made within the technical idea and principle of the present application should be included in the protection scope of the present application.
Claims (9)
1. The probiotic powder capable of preventing decayed teeth and refreshing oral cavity is characterized by comprising lactobacillus gasseri HHuMin D, lactobacillus fermentum OK, bifidobacterium bifidum BGN4, bifidobacterium lactis AD011, sugar alcohol sweetening agent and prebiotics; the lactobacillus gasseri HHuMin D, the lactobacillus fermentum OK, the bifidobacterium bifidum BGN4 and the bifidobacterium lactis AD011 are main adjusting components, and the prebiotics and the sugar alcohol sweetening agent are auxiliary adjusting components; wherein: the prebiotics comprise galacto-oligosaccharide, fructo-oligosaccharide and xylo-oligosaccharide, and the sugar alcohol sweetener comprises erythritol and xylitol.
2. The probiotic powder for preventing dental caries and refreshing oral cavity according to claim 1, wherein the probiotic powder comprises the following components in an addition amount of 2g per bag:
lactobacillus gasseri HHuMin D50 hundred million CFU/bag (counting according to viable count)
Lactobacillus fermentum OK 50 hundred million CFU/bag (in terms of viable count)
Bifidobacterium bifidum BGN 450 hundred million CFU/bag (counting live bacteria)
Bifidobacterium lactis AD 01150 hundred million CFU/bag (in terms of viable count)
Erythritol 100 mg/bag
Xylitol 100 mg/bag
Galacto-oligosaccharide 48 mg/bag
108 mg/bag of fructo-oligosaccharide
Xylo-oligosaccharide 16 mg/bag
The preparation method of the probiotic powder comprises the following steps:
1) preparation of freeze-dried powder
The protective agent is respectively mixed with the activated single pure bacterial strain and the bacterial liquid, and the mixture is poured into a container and placed in a vacuum freeze dryer to prepare 4 kinds of single probiotic freeze-dried bacterial powder.
2) Preparation of formula bacteria powder
Respectively taking 4 single probiotic freeze-dried powder according to the formula amount, mixing, sequentially adding erythritol, xylitol, galacto-oligosaccharide, fructo-oligosaccharide and xylo-oligosaccharide according to the formula amount, mixing, uniformly stirring, and subpackaging in bags to obtain the probiotic powder.
3. The probiotic powder for preventing dental caries and freshening oral cavity according to claim 2, wherein the protective agent is made up of the following ingredients in percentage by weight: 15% of skimmed milk powder, 6% of trehalose, 4% of glycerol and 1.5% of xylitol.
4. The probiotic powder for preventing dental caries and refreshing oral cavity according to claim 2, wherein the probiotic powder packaging bag is made of food-grade PE packaging material with good sealing performance and safety.
5. The probiotic powder for preventing caries and freshening the oral cavity according to claim 2, wherein said probiotic powder is administered in an amount of: after meal, after brushing teeth and before sleeping, the tea is drunk with water with the temperature lower than 37 ℃ or directly eaten.
6. The probiotic powder for preventing caries and freshening oral cavity according to claim 5, wherein said probiotic powder has a dietary recommendation of: for severe oral diseases, the food is taken for 1 month in 2 bags per day, and after improvement, the food is taken for two months in 1 bag per day.
7. The probiotic powder for preventing caries and freshening oral cavity according to claim 5, wherein the dietary recommendation for the probiotic powder is: for the patients with normal oral health, the food is taken for 3 months in 1 bag per day.
8. The probiotic powder for preventing caries and freshening oral cavity according to claim 5, wherein said probiotic powder has a dietary recommendation of: during the period of taking the antibiotics, the antibiotics are used firstly, and the probiotic powder is used at intervals of 2-3 hours.
9. Use of probiotic powder according to claim 1 or 2, for ameliorating the manifestations of oral problems due to pathogenic bacteria proliferation and bad breath problems due to dyspepsia.
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