CN107582573A - A kind of probiotics drops and preparation method thereof - Google Patents
A kind of probiotics drops and preparation method thereof Download PDFInfo
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- CN107582573A CN107582573A CN201710726835.9A CN201710726835A CN107582573A CN 107582573 A CN107582573 A CN 107582573A CN 201710726835 A CN201710726835 A CN 201710726835A CN 107582573 A CN107582573 A CN 107582573A
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Abstract
The present invention relates to probiotics preparation, more particularly to a kind of probiotics drops and preparation method thereof；Probiotics drops is in terms of mass fraction, including 1 2.5% unrighted acid, 85 90% vegetable oil, 1 7% phosphatide oil and 2 8% probiotics suspensions, and the probiotics suspension includes glycerine, Tween 80 and probio thalline；Preparation method comprises the following steps：S1, by probio thalline by a certain percentage, carry out combined balance system with glycerine, Tween 80, stand and reach system poised state in 15 30 minutes, probiotics suspension is made；S2, by probiotics suspension by a certain percentage, with unrighted acid, vegetable oil, phosphatide oil carry out it is fully emulsified uniformly, obtain probiotics drops；The probiotics drops of the present invention, can improve the Tiny ecosystem in smoking population oral cavity and nose, improve the resistance of smoking population.
Description
Technical field
The present invention relates to probiotics preparation, more particularly to a kind of probiotics drops and preparation method thereof.
Background technology
Smoke from cigarette adversely affects to health, long-term smoking can cause people suffer from lung cancer, COPD,
The risk increase of the diseases such as angiocardiopathy, periodontitis.In addition, microbiologic population in oral cavity and nose due to smoke from cigarette
Directly contact, influenceed by smog kind noxious material, its Tiny ecosystem ecology can also be a greater impact.
Probiotics is mainly formed as most lactic acid bacteria, small part bacillus and yeast, in the premise of certain intake
Under, host can be produced and improve numerous beneficial functionals such as immunity, regulation enteron aisle, balance body fat.In oral cavity field of probiotic bacteria, benefit
Raw bacterium can compete oral mucosa binding site with oral cavity pathogen, viscous on mouth and nose chamber hard and soft tissue so as to suppress pathogenic bacteria
Attached breeding, antibacterial substance, such as bacteriocin and hydrogen peroxide etc. can be also secreted to suppress the bacteria growing that causes a disease, finally adjusts respiratory tract
Colony balance, maintain normal microenvironment.
However, probiotics is strict to extraneous environmental requirement, significantly affected as Bifidobacterium has for oxygen stress, normal
High activity is kept when being difficult to long under temperature or hot environment, thus how to keep probiotic active for a long time, is preferably applied to improve and inhales
Microecosystem balance in cigarette crowd oral cavity and nose, improves the resistance of smoking population, turns into a problem.
The content of the invention
In order to solve the above technical problems, it is an object of the invention to provide micro- in a kind of improvement smoking population oral cavity and nose
The ecological balance, smoking population is improved to probiotics drops of common mouth and nose chamber disease resistance and preparation method thereof.
First aspect present invention provides a kind of probiotics drops, in terms of mass fraction, includes 1-2.5% unsaturated fat
Acid, 85-90% vegetable oil, 1-7% phosphatide oils and 2-8% probiotics suspensions, the probiotics suspension include the third three
Alcohol, Tween-80 and probio thalline.
Specifically, the one kind or more of the unrighted acid in oleic acid, linoleic acid, leukotrienes, arachidonic acid
Kind.
Specifically, the vegetable oil is selected from soybean oil, sesame oil, peanut oil, olive oil, sunflower oil, coconut oil, palm
One or more in oil.
Specifically, the phosphatide oil is selected from soyabean lecithin oil, modified soy bean lipoid oil, sunflower phosphatide oil, lecithin oil, flower
One or more in raw phosphatide oil.
Second aspect of the present invention provides a kind of preparation method of probiotics drops, comprises the following steps：
S1, by probio thalline by a certain percentage, with glycerine, Tween-80 carry out combined balance system, stand 15-30 minutes
System poised state is reached, probiotics suspension is made；
S2, by probiotics suspension by a certain percentage, with unrighted acid, vegetable oil, phosphatide oil carry out it is fully emulsified
Uniformly, probiotics drops is obtained.
Specifically, in the step S1, by glycerine and 0.001-0.5% Tween-80 that concentration is 2-50% with 1:1
Ratio uniform be mixed into mixed liquor, then by probio thalline and mixed liquor according to mass ratio 1:1-1:10, which carry out mixing, puts down
Weighing apparatus.
Preferably, in the step S1, by concentration be 10-20% glycerine and 0.05-0.5% Tween-80 with 1:1
Ratio uniform be mixed into mixed liquor, then by probio thalline and mixed liquor according to mass ratio 1:1-1:10, which carry out mixing, puts down
Weighing apparatus.
Further, the preparation method of the probio thalline, comprises the following steps：
S1, microorganism collection：Probiotics fermention liquid is aseptically cooled centrifugal treating, collects thalline；
S2, thalline washing purifying：Purifying is rinsed to thalline using phosphate buffer, removes and is metabolized in fermentation process
The residual of product and substrate, delay and close thalline pH and viscosity.
Further, the probiotics uses the probiotics separated from healthy human body oral cavity.
Specifically, the probiotics include streptococcus, lactobacillus, Bifidobacterium, gemma Pseudomonas, saccharomyces it is thin
Bacterium.
Specifically, the bacterium of the streptococcus includes streptococcus thermophilus, streptococcus salivarius, streptococcus fecalis, lactic acid sheet ball
Bacterium, Pediococcus pentosaceus and its mixture.
Done specifically, the bacterium of the lactobacillus includes Lactobacillus plantarum, Lactobacillus rhamnosus, Lactobacillus casei, pair
Lactobacillus paracasei, lactobacillus reuteri, lactobacillus acidophilus, lactobacillus bulgaricus, Lactobacillus salivarius, lactobacillus fermenti, grignard breast
Bacillus, Yue Shi lactobacillus, Lactobacillus Jensenii and its mixture.
Specifically, the bacterium of the Bifidobacterium includes bifidobacterium lactis, bifidobacterium longum, bifidobacterium breve, youth
Bifidobacterium, bifidobacterium infantis, bifidobacterium bifidum and their mixture.
Specifically, the bacterium of the bacillus includes bacillus coagulans.
Specifically, the cell of the Blastocystis includes Bu Ladi yeast.
Further, the unrighted acid, vegetable oil, phosphatide oil are sterilized using high pressure-temperature moist heat sterilization is preceding needed
Condition is 118-121 DEG C, and the time is 15-30 minutes.
Further, in the step S2, probiotics bacterial suspension need to control pH scope pH5.5-6.5, and emulsification balances laggard
The mesh of row 100 sieves.
By such scheme, the present invention at least has advantages below：
1) prevented to smoke product used by crowd as one kind, a variety of formulations be present in the market, such as：Tablet, pulvis,
Spray, granule, drops etc., probiotics drops involved in the present invention belong to oral suspensions, and this formulation main advantage is body
Product little Yi storages, it is convenient to take, and is applicable different age group crowd.
2) glycerine (glycerine), mixed phosphatide oil are used as Main System part in the present invention, and function is to be used as to have concurrently
Liposoluble and water-soluble and integral compounding substances；First by thalline mixing glycerine so that thalline temporary dispersion is in glycerol system,
Even scattered, equilibrated rear glycerine reaches good intersolubility with thalline；Transparent mixing class phosphatide is added, the thalline of balance is carried out
Parcel, it is fully emulsified after addition vegetable oil as drops carrier so that phosphatide and vegetable oil are dispersed, thalline is embedded,
Starvation, temperature；Glycerine and phosphatide oil are adhered in thalline outer surface, accelerate and are obviously improved dissolubility of the thalline in oil-based solvent
And the unit rate of settling；Probiotics has high resistance to cold and diseases and stability in drops, can adapt to the rings such as high oxygen concentration, high temperature
Border, deposit under room temperature or refrigeration (4-37 degrees Celsius), viable count can reach 10 in shelf life8Cfu/g, the shelf-life is up to 12-
18 months.
3) probiotics drops of the invention, directly orally or nose drop can be carried out, the caused mouth and nose chamber that can prevent to smoke is common
Persistent ailment, its main mechanism are to compete oral mucosa binding site using probiotics and oral cavity pathogen, suppress pathogenic bacteria and exist
Adhesion breeding on mouth and nose chamber hard and soft tissue, secondly Major Secretory antibacterial substance, such as bacteriocin and hydrogen peroxide etc. suppress
Cause a disease bacteria growing, final to adjust Respiratory tract microbial population balance, maintains normal microenvironment；As oiliness drops when in use more
It is easy to the adhesiveness and cultivation effect of probiotics counterpart nasal epithelia.
4) probiotics drops of the invention, outward appearance are translucent shape, oleaginous system, free from extraneous odour and conglomeration precipitation, are guaranteeing the quality
Occurred without in phase it is any it is mouldy, become sour, change colour, foreign matter, produce gas and other denaturalization phenomenons.Suspension probiotic ingredient uniformly divides
Dissipate, sedimentation volume ratio meets national Specification.
Described above is only the general introduction of technical solution of the present invention, in order to better understand the technological means of the present invention,
And can be practiced according to the content of specification, below with presently preferred embodiments of the present invention and coordinate accompanying drawing describe in detail as after.
Brief description of the drawings
Fig. 1 be in probiotics drops active thalline in 12 months stability diagrams of normal temperature；
Fig. 2 is inhibition figure of the probiotic cell constituent to oral cavity pathogen adhesion function.
Embodiment
With reference to the accompanying drawings and examples, the embodiment of the present invention is described in further detail.Implement below
Example is used to illustrate the present invention, but is not limited to the scope of the present invention.
Terminology used in the present invention " probiotics ", main composition is lactic acid bacteria, Gram-positive, anaerobism or amphimicrobian are thin
Bacterium, can ferment different carbohydrate production acids, and the main acids type that produces is lactic acid, acetic acid, propionic acid.The conventional probiotics of industry has newborn bar
Bacterium, streptococcus, leukonid, Lactococcus lactis, brevibacterium.Bifidobacterium belongs to strict anaerobes, is also commonly used for and lactic acid bacteria
Compounding use is as probiotics preparation, included in lactic acid bacteria flora.
Term " thalline ", is referred mainly to after probiotics fermention terminates, and zymotic fluid carries out solid-liquid through bio-separation extractive technique
Separation is collected to be obtained, and conventional centrifugation or membrane filtration are separated and collected, white in uneven thick liquid or solid form
Or faint yellow, free from admixture.
Embodiment 1：
The present embodiment provides a kind of probiotics drops with the crowd's mouth nasal cavity disease that prevents to smoke, per 1000g probiotics
In drops, required addition each component dosage is：Lactobacillus salivarius Lactobacillus salivarius bacteria suspensions：20-80g
(comprising glycerine 7.5-30g, Tween-80 0.075-0.3g, remaining be thalline), palm oil 300g, sunflower oil 550-600g,
Non-transgenic soybean phosphatidase 1 0-70g, linoleic acid 10-25g.
Wherein, the addition of cell composition milkiness thing is mainly influenceed in terms of using effect, fixed to suppressing pathogen adhesion
The effect of growing effect.Sunflower oil mainly influences the sedimentation speed of cell composition milkiness thing in the product with linoleic addition
Rate, dispersed homogeneous degree and odor impact.The addition of soybean lecithin can cause to change to product mobility and color and luster, high dose
Addition can cause product colour deepen and viscosity improve, conversely, its color and luster depth of low dosage and viscosity relative reduction.
Lactobacillus salivarius Lactobacillus salivarius are probiotics, Gram-positive, do not generate spore, do not have
Catalase, oxidizing ferment and motility, it can be grown in aerobic and anaerobic environment, belong to facultative heterolactic turning sour property bacterial strain, glucose metabolism
When do not produce gas.Salivarius means that " body of gland type " is initially by being found in human glandular, is in human body alimentary canal
Primary strain, also it is that food and medicine Surveillance Authority of the U.S. (FDA) table arranges one of safe strain.
The probiotics drops of this implementation, specific preparation method are as follows：
S1, using from separation screening Lactobacillus salivarius in healthy human body of gland, carry out purifies and separates, and pass through 16s
RDNA complete sequences are accredited as Lactobacillus salivarius Lactobacillus salivarius, and carry out glycerol tube preservation.
S2, freezing glycerol tube carried out MRS medium optimizations, continuous squamous subculture 3 times and carry out being amplified to more than 10L and send out
Ferment volume.
S3, control fermentation key factor carry out High Density Cultivation in fermentation tank to Lactobacillus salivarius
S4, it will ferment and complete that bacterium solution is collected, collection mode can be used and is collected by centrifugation or membrane filtration, micro-filtration, nanofiltration
Deng collection mode.
S5, gained thalline carry out purifying rinse using phosphate buffer PBS, remove soluble remaining substrate and ferment generation
Thank to product.
S6, by 50% glycerine and Tween-80 with 1:1 ratio mixing, then by thalline after purification and mixed liquor with 1:3
Mixing, it is sufficiently stirred emulsification and controls temperature to be less than 25 degrees Celsius in emulsion process.It is Celsius that 4 are positioned over after emulsification
Degree insulation 30 minutes, makes its glycerol system reach complete equipilibrium.
S7, gained bacteria suspension are added in mixed vegetable oil according to addition 2.0-8.0%, described according to application claims
Vegetable oil is palm oil and sunflower oil mixture and is modified oily sunflower phosphatide comprising 0.5%-1.5%, and specific formula is visible
Table 1.
The probiotics drops formula of table 1
Smoking population disease, human body is referred mainly in smoking process, a large amount of intakes contain nicotine, tar, benzene class and ammonia
Class compound, its most materials have carcinogenesis, and long-term suction can cause many places such as respiratory tract, esophagus, intestines and stomach, heart to damage
Wound even suffers from the diseases such as rhinitis, pharyngitis and cancer of the esophagus, lung cancer, causes physical function significantly to damage.
The probiotics drops of the present embodiment can be adhered in respiratory tract, esophagus as viable type drops product first,
And carrying out colonizing adhesion in the environment of air, forming epithelial cell external shield, carrying out metabolism synthesis, suppress cause of disease
Bacterium and pathogenic bacteria colonize and grown.Secondly dispersed system, somatic cells are formed in product storage process in unsaturated lipid
Microcapsules cluster is formed under fat acid and oil components, there is the high stability under normal temperature environment.
Embodiment 2：
A kind of potential allergy derived components such as no soybean of the present embodiment offer have the crowd's mouth nasal cavity disease that prevents to smoke
Probiotics drops, specific formula can be shown in Table 2；Preparation method repeats no more with reference to the operation of the preparation method of embodiment 1.
Second of the probiotics drops formula of table 2
The probiotics drops of the present embodiment, take 25 degrees Celsius of normal temperature and 37 celsius temperatures carry out ordinary temperature stability with
Track, data acquisition results are as shown in figure 1, normal temperature keeps viable count to can reach 10 in lower 18 months8Cfu/g, also can be 8 under high temperature
Viable count is kept to can reach 10 in month8cfu/g。
Cell composition milkiness thing is compound probiotic formula in the probiotics drops of the present embodiment, normal for mouth nasal cavity disease
See that pathogen adhesion is effectively reduced, reduce pathogenic bacteria colonization ability, improve epithelial cell and colonize drag, as shown in Fig. 2
Add a certain proportion of cell composition milkiness thing can effect reduce the streptococcic adhesive capacity of beads in oral cavity, oral candidiasis chain
Coccus has compared with high abundance in the mouth epithelial cells of common disease crowd as a kind of pathogenic bacteria, reduces its adhesive capacity
It can effectively reduce and colonize effect, wherein Lactobacillus rhamnosus LGG is as reference group.
The probiotics drops of the present embodiment, wherein formula components are free of allergy derived components, are directed to special population allergy people
Group carries out improvement upgrading, there is provided extensively using field and applicable crowd.
Described above is only the preferred embodiment of the present invention, is not intended to limit the invention, it is noted that for this skill
For the those of ordinary skill in art field, without departing from the technical principles of the invention, can also make it is some improvement and
Modification, these improvement and modification also should be regarded as protection scope of the present invention.
Claims (10)
- A kind of 1. probiotics drops, it is characterised in that：In terms of mass fraction, including 1-2.5% unrighted acid, 85- 90% vegetable oil, 1-7% phosphatide oils and 2-8% probiotics suspensions, the probiotics suspension include glycerine, tween- 80 and probio thalline.
- 2. probiotics drops according to claim 1, it is characterised in that：The unrighted acid is selected from oleic acid, sub- oil One or more in acid, leukotrienes, arachidonic acid.
- 3. probiotics drops according to claim 1, it is characterised in that：The vegetable oil is selected from soybean oil, sesame oil, flower One or more in oil generation, olive oil, sunflower oil, coconut oil, palm oil.
- 4. probiotics drops according to claim 1, it is characterised in that：The phosphatide oil is selected from soyabean lecithin oil, modification One or more in soyabean lecithin oil, sunflower phosphatide oil, lecithin oil, peanut phosphatide oil.
- 5. the preparation method of the probiotics drops according to any one of Claims 1-4, it is characterised in that：Including following step Suddenly：S1, by probio thalline by a certain percentage, with glycerine, Tween-80 carry out combined balance system, stand 15-30 minutes make it Reach system poised state, probiotics suspension is made；S2, by probiotics suspension by a certain percentage, with unrighted acid, vegetable oil, phosphatide oil carry out it is fully emulsified uniformly, Obtain probiotics drops.
- 6. the preparation method of the probiotics drops according to any one of claim 5, it is characterised in that：In the step S1, By concentration be 2-50% glycerine and 0.001-0.5% Tween-80 with 1:1 ratio uniform is mixed into mixed liquor, then By probio thalline and mixed liquor according to mass ratio 1:1-1:10 carry out combined balance system.
- 7. the preparation method of the probiotics drops according to any one of claim 5, it is characterised in that：The probio thalline Preparation method, comprise the following steps：S1, microorganism collection：Probiotics fermention liquid is aseptically cooled centrifugal treating, collects thalline；S2, thalline washing purifying：Purifying is rinsed to thalline using phosphate buffer, removes metabolite in fermentation process And the residual of substrate, delay and close thalline pH and viscosity.
- 8. the preparation method of the probiotics drops according to any one of claim 5, it is characterised in that：The probiotics uses The probiotics separated from healthy human body oral cavity.
- 9. the preparation method of the probiotics drops according to any one of claim 5, it is characterised in that：The unsaturated fat Acid, vegetable oil, phosphatide oil are using HTHP moist heat sterilization is preceding needed, and sterilising conditions are 118-121 DEG C, and the time is 15-30 minutes.
- 10. the preparation method of the probiotics drops according to any one of claim 5, it is characterised in that：In the step S2, Probiotics bacterial suspension need to control pH scope pH5.5-6.5, and 100 mesh sievings are carried out after emulsification balance.
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108524552A (en) * | 2018-03-08 | 2018-09-14 | 江苏微康生物科技有限公司 | A kind of preparation method of probiotics controlled release dripping pill |
| CN109619214A (en) * | 2019-01-15 | 2019-04-16 | 广州富诺健康科技股份有限公司 | A kind of mushroom oil droplet agent and preparation method thereof rich in vegetalitas VD2 |
| CN110037127A (en) * | 2019-04-23 | 2019-07-23 | 彤博士健康产业河北有限公司 | A kind of probiotics preparation and preparation method thereof improving infant's enteron aisle |
| CN111110704A (en) * | 2020-02-14 | 2020-05-08 | 上海城建职业学院 | Probiotic agent and drop as well as preparation method and application thereof |
| CN112056399A (en) * | 2020-06-28 | 2020-12-11 | 武汉康复得生物科技股份有限公司 | Probiotic composition for enhancing immunity and application thereof |
| CN112263597A (en) * | 2020-11-06 | 2021-01-26 | 拉格比(广东)健康科技有限公司 | Probiotic drop for improving allergic constitution |
| WO2021212108A1 (en) * | 2020-04-17 | 2021-10-21 | Advanced Biological Marketing, Inc. | Method of stable formulation of microbial and microbe derived products and use of same |
| CN114796287A (en) * | 2022-06-09 | 2022-07-29 | 比菲德(北京)生物科技有限公司 | Probiotic powder capable of preventing decayed teeth and refreshing oral cavity and application thereof |
| CN115088844A (en) * | 2022-07-14 | 2022-09-23 | 河北益存生物科技有限公司 | Lactic acid bacteria oil dropping liquid and preparation method thereof |
| CN115531424A (en) * | 2021-06-30 | 2022-12-30 | 锦乔生物科技有限公司 | Probiotic drops |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104922157A (en) * | 2014-09-11 | 2015-09-23 | 柳志信 | Microbial ecological preparation nasal drops |
| CN105707897A (en) * | 2016-02-02 | 2016-06-29 | 上海交大昂立股份有限公司 | Probiotic lipid suspension drop and preparation method thereof |
| CN106497854A (en) * | 2017-01-19 | 2017-03-15 | 南京农业大学 | Lactobacilluss D8 and its application |
| CN106794137A (en) * | 2014-06-10 | 2017-05-31 | 诺舒易专利有限公司 | The improvement of nasal composition and its application method |
| CN106822007A (en) * | 2015-09-11 | 2017-06-13 | 西姆莱斯股份公司 | Oral formulations |
-
2017
- 2017-08-23 CN CN201710726835.9A patent/CN107582573A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106794137A (en) * | 2014-06-10 | 2017-05-31 | 诺舒易专利有限公司 | The improvement of nasal composition and its application method |
| CN104922157A (en) * | 2014-09-11 | 2015-09-23 | 柳志信 | Microbial ecological preparation nasal drops |
| CN106822007A (en) * | 2015-09-11 | 2017-06-13 | 西姆莱斯股份公司 | Oral formulations |
| CN105707897A (en) * | 2016-02-02 | 2016-06-29 | 上海交大昂立股份有限公司 | Probiotic lipid suspension drop and preparation method thereof |
| CN106497854A (en) * | 2017-01-19 | 2017-03-15 | 南京农业大学 | Lactobacilluss D8 and its application |
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108524552A (en) * | 2018-03-08 | 2018-09-14 | 江苏微康生物科技有限公司 | A kind of preparation method of probiotics controlled release dripping pill |
| CN109619214A (en) * | 2019-01-15 | 2019-04-16 | 广州富诺健康科技股份有限公司 | A kind of mushroom oil droplet agent and preparation method thereof rich in vegetalitas VD2 |
| CN110037127A (en) * | 2019-04-23 | 2019-07-23 | 彤博士健康产业河北有限公司 | A kind of probiotics preparation and preparation method thereof improving infant's enteron aisle |
| CN111110704A (en) * | 2020-02-14 | 2020-05-08 | 上海城建职业学院 | Probiotic agent and drop as well as preparation method and application thereof |
| CN111110704B (en) * | 2020-02-14 | 2023-03-28 | 上海城建职业学院 | Probiotic agent and drop as well as preparation method and application thereof |
| WO2021212108A1 (en) * | 2020-04-17 | 2021-10-21 | Advanced Biological Marketing, Inc. | Method of stable formulation of microbial and microbe derived products and use of same |
| CN112056399A (en) * | 2020-06-28 | 2020-12-11 | 武汉康复得生物科技股份有限公司 | Probiotic composition for enhancing immunity and application thereof |
| CN112263597A (en) * | 2020-11-06 | 2021-01-26 | 拉格比(广东)健康科技有限公司 | Probiotic drop for improving allergic constitution |
| WO2022095287A1 (en) * | 2020-11-06 | 2022-05-12 | 拉格比(广东)健康科技有限公司 | Probiotic drops for improving allergic constitution |
| CN115531424A (en) * | 2021-06-30 | 2022-12-30 | 锦乔生物科技有限公司 | Probiotic drops |
| TWI800860B (en) * | 2021-06-30 | 2023-05-01 | 豐華生物科技股份有限公司 | Probiotic drop |
| CN114796287A (en) * | 2022-06-09 | 2022-07-29 | 比菲德(北京)生物科技有限公司 | Probiotic powder capable of preventing decayed teeth and refreshing oral cavity and application thereof |
| CN115088844A (en) * | 2022-07-14 | 2022-09-23 | 河北益存生物科技有限公司 | Lactic acid bacteria oil dropping liquid and preparation method thereof |
| CN115088844B (en) * | 2022-07-14 | 2024-05-24 | 河北益存生物科技有限公司 | Lactic acid bacteria oil drop and preparation method thereof |
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