CN114794025B - Device and method for testing hypoxia stress of stored-grain insects - Google Patents
Device and method for testing hypoxia stress of stored-grain insects Download PDFInfo
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- CN114794025B CN114794025B CN202210486115.0A CN202210486115A CN114794025B CN 114794025 B CN114794025 B CN 114794025B CN 202210486115 A CN202210486115 A CN 202210486115A CN 114794025 B CN114794025 B CN 114794025B
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- 238000012360 testing method Methods 0.000 title claims abstract description 102
- 241000238631 Hexapoda Species 0.000 title claims abstract description 68
- 206010021143 Hypoxia Diseases 0.000 title claims abstract description 23
- 230000007954 hypoxia Effects 0.000 title claims abstract description 23
- 238000000034 method Methods 0.000 title claims abstract description 18
- 230000007246 mechanism Effects 0.000 claims abstract description 61
- 238000012545 processing Methods 0.000 claims abstract description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 24
- 230000005540 biological transmission Effects 0.000 claims description 13
- 230000001105 regulatory effect Effects 0.000 claims description 13
- 238000007789 sealing Methods 0.000 claims description 13
- 238000002347 injection Methods 0.000 claims description 10
- 239000007924 injection Substances 0.000 claims description 10
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical class [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 7
- 238000009662 stress testing Methods 0.000 claims 1
- 239000007789 gas Substances 0.000 abstract description 45
- 239000001301 oxygen Substances 0.000 abstract description 9
- 229910052760 oxygen Inorganic materials 0.000 abstract description 9
- 241000607479 Yersinia pestis Species 0.000 description 7
- 230000008569 process Effects 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 239000000243 solution Substances 0.000 description 3
- 230000006978 adaptation Effects 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000003938 response to stress Effects 0.000 description 2
- FGRBYDKOBBBPOI-UHFFFAOYSA-N 10,10-dioxo-2-[4-(N-phenylanilino)phenyl]thioxanthen-9-one Chemical compound O=C1c2ccccc2S(=O)(=O)c2ccc(cc12)-c1ccc(cc1)N(c1ccccc1)c1ccccc1 FGRBYDKOBBBPOI-UHFFFAOYSA-N 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/51—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture specially adapted for storing agricultural or horticultural products
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Testing Resistance To Weather, Investigating Materials By Mechanical Methods (AREA)
Abstract
The invention discloses a grain storage insect hypoxia stress test device and method, comprising a test box body, wherein a transverse plate is fixedly connected in the test box body, the transverse plate divides the test box body into a processing cavity and an operating cavity, the processing cavity is positioned above the operating cavity, a plurality of through holes are formed in the transverse plate, the processing cavity is communicated with the operating cavity through the through holes, a plurality of insect observation mechanisms are arranged in the processing cavity and are respectively and correspondingly arranged with the through holes, a humidity control mechanism, a gas control mechanism and a pressure control mechanism are arranged on the test box body, the humidity control mechanism, the gas control mechanism and the pressure control mechanism are respectively and correspondingly arranged in the test box body, a box cover is hinged to the top end of the test box body, a pressing mechanism is arranged on the box cover, and the box cover is in limit fit with the test box body through the pressing mechanism. The invention is suitable for researching the reaction of the grain storage insect under the normal pressure condition to the low-oxygen environment under the conditions of different humidity and gas concentration, and the sample can be accessed and checked at any time, thereby being convenient for the low-oxygen stress test of the grain storage insect.
Description
Technical Field
The invention relates to the technical field of insect tests, in particular to a device and a method for testing hypoxia stress of stored-grain insects.
Background
The pest in stored grain is one of the important factors for damaging the safety of stored grain in China, the pest in stored grain is controlled by using a low-oxygen regulating technology, so that the pest in stored grain is gradually promoted and applied in China, but if the gas regulating is once out of the full-automatic grain regulating technology, the pest in stored grain can cause great adverse effects, the pest in stored grain is lost due to missing of the pest killing time, the gas regulating resistance of the pest in stored grain can be caused, and the difficulty of gas regulating application is increased. In the prior art, when the low oxygen stress test of the stored grain insects is carried out, the sample access inspection needs a plurality of switch devices, the sample is inconvenient to take, the test efficiency is influenced, the observation of the low oxygen stress test insect sample of the stored grain insects is inconvenient to carry out, and meanwhile, the insect stress response is larger during the test, so that the test accuracy is influenced. Therefore, there is a need for a device and a method for testing the hypoxia stress of stored-grain insects, which are used for testing and researching the survivability of different stored-grain insects in a hypoxia environment.
Disclosure of Invention
The invention aims to provide a device and a method for testing hypoxia stress of stored-grain insects, which are used for solving the problems in the prior art.
In order to achieve the above object, the present invention provides the following solutions: the invention provides a grain storage insect hypoxia stress test device which comprises a test box body, wherein a transverse plate is fixedly connected in the test box body, the transverse plate divides the test box body into a processing cavity and an operating cavity, the processing cavity is positioned above the operating cavity, a plurality of through holes are formed in the transverse plate, the processing cavity and the operating cavity are communicated through the through holes, a plurality of insect observation mechanisms are arranged in the processing cavity and are respectively and correspondingly arranged with the through holes, a humidity control mechanism, a gas control mechanism and a pressure control mechanism are arranged on the test box body, the humidity control mechanism, the gas control mechanism and the pressure control mechanism are respectively and correspondingly arranged in the test box body, a box cover is hinged to the top end of the test box body, a pressing mechanism is arranged on the box cover, and the box cover is in limit fit with the test box body through the pressing mechanism.
Preferably, the insect observation mechanism comprises a disc fixedly connected to the inner wall of the processing cavity, an annular chute is formed in the front end of the disc, the center point of the annular chute is staggered with the center point of the disc, a transmission shaft is rotationally connected to the middle of the disc, one end of the transmission shaft extends out of the outer fixedly connected with a crank of the test box body, the other end of the transmission shaft is fixedly connected with a bearing piece, the bearing piece is correspondingly arranged with the annular chute, a plurality of observation box placement frames are arranged on the bearing piece, an observation box is detachably connected to the observation box placement frames, and the observation boxes are correspondingly arranged with the through holes.
Preferably, the bearing piece comprises a bearing disc fixedly connected to the transmission shaft, the center point of the bearing disc is coaxially arranged with the center point of the disc, six supporting rods are fixedly connected to the outer wall of the bearing disc at equal intervals along the circumferential direction, the supporting rods are far away from one end of the bearing disc and are rotationally connected with a supporting shaft, the observing box placing frame is fixedly connected to one end of the supporting shaft, one end of the connecting plate is fixedly connected to the other end of the supporting shaft, the other end of the connecting plate is hinged to a sliding block, the sliding block is matched with the annular sliding groove, a connecting rod is arranged on the observing box placing frame, and two ends of the connecting rod are respectively hinged to the adjacent two observing box placing frames.
Preferably, the top end of the observation box is detachably connected with a box cover, the bottom end of the observation box is slidably connected with a bottom plate, and a plurality of vent holes are formed in the box cover, the bottom plate and the side wall of the observation box.
Preferably, the humidity control mechanism comprises a humidity control box fixedly connected to the inner wall of the processing cavity, a water injection hole is formed in the outer side wall of the test box, the humidity control box is communicated with the water injection hole, and a sealing plug is arranged on the water injection hole.
Preferably, the gas control mechanism comprises a gas inlet and a gas outlet which are formed in the outer side wall of the test box body, the gas inlet and the gas outlet are communicated with the processing cavity, the gas inlet is fixedly connected with an external gas cylinder through a pipeline, the gas outlet is fixedly connected with a gas concentration detector through a pipeline, and gas switching valves are arranged on the gas inlet and the gas outlet.
Preferably, the pressure control mechanism comprises a U-shaped pressure gauge fixedly connected to the test box body, one end of the U-shaped pressure gauge is communicated with the air outlet through a pipeline, and the other end of the U-shaped pressure gauge is open.
Preferably, the pressing mechanism comprises two fixing seats fixedly connected to the front end of the box cover, two hooks are fixedly connected to the front end of the test box body, and the hooks are matched with the fixing seats.
Preferably, two holes are formed in the front end of the test box body, the holes are communicated with the operation cavity, sealing gloves are fixedly connected to the holes, a threaded opening is formed in the outer side wall of the test box body, the threaded opening is communicated with the operation cavity, and a threaded cover plate is connected to the threaded opening in a threaded mode.
The method is suitable for a grain storage insect hypoxia stress test device, and comprises the following steps:
a. placing insects to be tested: injecting clear water or saturated saline water into the humidity control mechanism, and placing insects with consistent growth states and numbers into the insect observation mechanism;
b. adjusting the gas concentration: regulating the gas concentration in the processing cavity through a gas control mechanism;
c. and (3) performing sealing observation: step a, volatilizing clear water or saturated saline water to a target value, and sealing a test box body for observation;
d. adjusting the pressure: the internal pressure of the test box body is regulated by a pressure control mechanism;
e. taking out a sample and observing: insect samples were taken from the test chamber for observation.
The invention discloses the following technical effects:
the invention is suitable for researching the reaction of the grain storage insect to the low-oxygen environment under the conditions of different humidity and gas concentration under the normal pressure condition, the sample can be accessed and checked at any time, and the observation test can be carried out on a plurality of groups of insects, meanwhile, the stress reaction of the insects is reduced in the observation process, the accuracy of test data is ensured, the low-oxygen stress test of the grain storage insect is convenient to carry out, the test effect is ensured, and the test efficiency is improved.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions of the prior art, the drawings that are needed in the embodiments will be briefly described below, it being obvious that the drawings in the following description are only some embodiments of the present invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of the front structure of the device of the present invention;
FIG. 2 is a schematic side view of the apparatus of the present invention;
FIG. 3 is a schematic view of the internal structure of the device of the present invention;
FIG. 4 is an enlarged view of a portion of FIG. 3A in accordance with the present invention;
FIG. 5 is a schematic view of the structure of the annular chute of the present invention;
FIG. 6 is a schematic view of a carrier structure according to the present invention;
FIG. 7 is a schematic view of the structure of the observation box of the present invention;
FIG. 8 is a schematic view of the connection state structure of the connecting rod of the present invention;
1, a test box body; 2. a cross plate; 3. a processing chamber; 4. an operating chamber; 5. a through hole; 6. a case cover; 7. a disc; 8. an annular chute; 9. a transmission shaft; 10. a crank; 11. an observation box placement frame; 12. a carrying tray; 13. a support rod; 14. a support shaft; 15. a connecting plate; 16. a slide block; 17. a box cover; 18. a bottom plate; 19. a humidity control box; 20. a water injection hole; 21. a sealing plug; 22. an air inlet; 23. an air outlet; 24. an external gas cylinder; 25. a gas concentration detector; 26. a gas switching valve; 27. a U-shaped pressure gauge; 28. a fixing seat; 29. a hook; 30. a hole; 31. a threaded opening; 32. a threaded cover plate; 33. and (5) connecting a rod.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
In order that the above-recited objects, features and advantages of the present invention will become more readily apparent, a more particular description of the invention will be rendered by reference to the appended drawings and appended detailed description.
The invention provides a grain storage insect hypoxia stress test device which comprises a test box body 1, wherein a transverse plate 2 is fixedly connected in the test box body 1, the transverse plate 2 divides the test box body 1 into a processing cavity 3 and an operation cavity 4, the processing cavity 3 is positioned above the operation cavity 4, a plurality of through holes 5 are formed in the transverse plate 2, the processing cavity 3 and the operation cavity 4 are communicated through the through holes 5, through hole covers are connected to the through holes 5 in a threaded manner and are used for dividing the processing cavity 3 and the operation cavity 4 into two sealed cavities, a plurality of insect observation mechanisms are arranged in the processing cavity 3 and are respectively arranged corresponding to the through holes, a humidity control mechanism, a gas control mechanism and a pressure control mechanism are arranged on the test box body 1, the humidity control mechanism, the gas control mechanism and the pressure control mechanism are all arranged corresponding to the inside of the test box body 1, a box cover 6 is hinged to the top end of the test box body 1, a pressing mechanism is arranged on the box cover 6, and the box cover 6 is in limit fit with the test box body 1 through the pressing mechanism.
Further optimizing scheme, in order to be convenient for observe the insect, the insect observation mechanism includes disc 7 of fixed connection on the inner wall of process chamber 3, annular spout 8 has been seted up to disc 7 front end, the central point of annular spout 8 and the crisscross setting of central point of disc 7, the middle part rotation of disc 7 is connected with transmission shaft 9, transmission shaft 9 one end stretches out test box 1 external fixation and is connected with crank 10, transmission shaft 9 other end fixedly connected with carrier, carrier and annular spout 8 correspond the setting, be provided with a plurality of observation box on the carrier and place frame 11, it is connected with the observation box to dismantle on the observation box place frame 11, it corresponds the setting with through-hole 5 to observe the box, simultaneously in order to be convenient for observe, test box 1, case lid 6, observe the box etc. and be transparent material, place the insect in the observation box, then will observe the box and place on the carrier, be convenient for test observation is carried out to the different group insects in later stage.
Further optimizing scheme, in order to realize the observation to the insect in the different observation boxes, the carrier includes the loading tray 12 of fixed connection on transmission shaft 9, loading tray 12 central point and the coaxial setting of central point of disc 7, it is six bracing pieces 13 to follow circumference equidistant fixedly connected with on the loading tray 12 outer wall, the one end rotation that loading tray 12 was kept away from to bracing piece 13 is connected with back shaft 14, the frame 11 is placed to the observation box fixed connection in back shaft 14 one end, the one end of back shaft 14 other end fixedly connected with connecting plate 15, the other end of connecting plate 15 articulates there is slider 16, slider 16 and annular spout 8 looks adaptation, it is connected with connecting rod 33 to rotate on the frame 11 to observe the box, the rotation point of connecting rod 33 is located the frame 11 middle part one side is placed to the observation box, six observation boxes are placed the frame 11 and divide into three groups, two observation boxes of every group place the frame 11, set up a connecting rod 33 between two observation boxes of every group place the frame 11, when the slider 16 slides in annular spout 8, through the connecting rod 33 between two boxes place the frame 11 guarantee that the observation box is placed the frame 11 is in the horizontal direction forever, the observation box is placed in the horizontal direction, the box that makes the box that sets up is always when rotating and observe the steady, the observation box is in the state when observing, the observation box is kept in the horizontal stress, when observing, the insect is observed.
Further optimizing scheme, for the access insect of being convenient for, survey the box top and can dismantle and be connected with lid 17, put into the insect through opening lid 17, survey box bottom sliding connection and have bottom plate 18, accessible whole observation box of taking off also accessible slip bottom plate 18 takes out inside insect when taking a sample, lid 17, bottom plate 18 and survey all offer a plurality of air vents on box placement frame 11 lateral wall and guarantee to survey the circulation of box and outside air.
Further optimizing scheme, in order to realize the regulation and the record to inside humidity, humidity control mechanism includes humidity control case 19 of fixed connection on the inner wall of process chamber 3, has seted up water injection hole 20 on the test box 1 lateral wall, humidity control case 19 and water injection hole 20 intercommunication are provided with sealing plug 21 on the water injection hole 20, fixedly connected with humidity detector on the test box 1 lateral wall for the humidity data in the record test box 1.
Further optimizing scheme, in order to realize the control to gas, gas control mechanism is including seting up air inlet 22 and gas outlet 23 on the lateral wall of test box 1, and air inlet 22 and gas outlet 23 all communicate with process chamber 3, and air inlet 22 passes through pipeline fixedly connected with outside gas cylinder 24, and gas outlet 23 passes through pipeline fixedly connected with gas concentration detector 25, all is provided with gas switch valve 26 on air inlet 22 and the gas outlet 23.
In order to realize the control of the internal pressure, the pressure control mechanism comprises a U-shaped pressure gauge 27 fixedly connected to the test box body 1, one end of the U-shaped pressure gauge 27 is communicated with the air outlet 23 through a pipeline, and the other end of the U-shaped pressure gauge 27 is open.
Further optimizing scheme, hold-down mechanism includes two fixing bases 28 of fixed connection at case lid 6 front end, and test box 1 front end fixedly connected with two couples 29, couple 29 and fixing base 28 looks adaptation.
Further optimizing scheme, in order to be convenient for test operation, two holes 30 have been seted up to test box 1 front end, and hole 30 and operation chamber 4 intercommunication all fixedly connected with sealed gloves on two holes 30, set up threaded opening 31 on test box 1 lateral wall, threaded opening 31 and operation chamber 4 intercommunication, threaded opening 31 go up threaded connection has screw cap 32.
The method is suitable for a grain storage insect hypoxia stress test device, and comprises the following steps:
a. placing insects to be tested: adding a certain amount of clear water or saturated saline water into the humidity control box 19, placing insects with the same growth state and number into the observation box, placing the observation box on the observation box placing frame 11, and closing the box cover 6;
b. adjusting the gas concentration: the gas concentration in the processing cavity 3 is regulated through an external gas cylinder 24, and the value of a gas concentration detector 25 is observed to be a required value;
c. and (3) performing sealing observation: the internal humidity is regulated by volatilizing clear water or saturated saline in the humidity control box 19, the humidity value is observed by a humidity detector, insects in the sealed test box body 1 are observed after the humidity value is regulated to a required value, and meanwhile, the insects in different observation boxes can be observed by rotating the crank 10;
d. adjusting the pressure: the air outlet 23 is opened to adjust the internal pressure of the test box body 1 through the U-shaped pressure gauge 27;
e. taking out a sample and observing: when the sample needs to be taken out, the observation box is taken out from the observation box placement frame 11 through the sealing glove or the insect sample is directly taken out from the observation box, and the screw cover plate 32 is opened to take out the insect sample.
According to the low-oxygen stress test device and method for the grain storage insects, when the low-oxygen stress test device and method are used, a certain amount of clear water or saturated saline water is added into a humidity control box 19, insects to be tested with the same growth state and the same number are placed in an observation box, the observation box is placed on an observation box placing frame 11, a box cover 6 is covered, an external air bottle 24 is connected, an air inlet 22 and an air outlet 23 are opened, the numerical value of a gas concentration detector 25 is observed, when the concentration obtains the target concentration, the air inlet 22 and the air outlet 23 are closed, the humidity in the test box 1 is regulated, the surface area of water in the humidity control box 19 is controlled, when the concentration of the humidity gas reaches the target value, the air outlet 23 is opened, the pressure in the test box 1 is controlled to be equal to the atmospheric pressure, when the samples are required to be taken out for observation, the covers in the middle of a transverse plate 2 are opened, the samples are taken out from a processing cavity 3 through a sealing glove, the samples are taken out from the processing cavity 4 through a threaded cover plate 32.
According to the device and the method for the hypoxia stress test of the stored grain insects, provided by the invention, a certain number of stored grain insects are raised, the reaction of the stored grain insects to the hypoxia environment under the conditions of different humidity and gas concentration can be studied, the sample can be checked at any time, meanwhile, the stress response of the insects is reduced in the observation process, the accuracy of test data is ensured, the hypoxia stress test of the stored grain insects is facilitated, the test effect is ensured, and the test efficiency is improved.
In the description of the present invention, it should be understood that the terms "longitudinal," "transverse," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like indicate or are based on the orientation or positional relationship shown in the drawings, merely to facilitate description of the present invention, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be construed as limiting the present invention.
The above embodiments are only illustrative of the preferred embodiments of the present invention and are not intended to limit the scope of the present invention, and various modifications and improvements made by those skilled in the art to the technical solutions of the present invention should fall within the protection scope defined by the claims of the present invention without departing from the design spirit of the present invention.
Claims (8)
1. The utility model provides a grain storage insect hypoxia stress test device, its characterized in that includes test box (1), fixedly connected with diaphragm (2) in test box (1), diaphragm (2) will test box (1) divide into treatment chamber (3) and operating chamber (4), treatment chamber (3) are located operating chamber (4) top, a plurality of through-holes (5) have been seted up on diaphragm (2), treatment chamber (3) with operating chamber (4) are through-hole (5) intercommunication, be provided with a plurality of insect observation mechanism in treatment chamber (3), a plurality of insect observation mechanism respectively with a plurality of through-holes correspond the setting, be provided with humidity control mechanism, gas control mechanism and pressure control mechanism on test box (1), humidity control mechanism, gas control mechanism and pressure control mechanism all with test box (1) are inside to be set up correspondingly, test box (1) top articulates there is case lid (6), be provided with on case lid (6) hold-down mechanism hold down mechanism (6) hold down mechanism hold down case (1) pass through limit device;
the insect observation mechanism comprises a disc (7) fixedly connected to the inner wall of the processing cavity (3), an annular chute (8) is formed in the front end of the disc (7), the center point of the annular chute (8) and the center point of the disc (7) are arranged in a staggered mode, the middle of the disc (7) is rotationally connected with a transmission shaft (9), one end of the transmission shaft (9) extends out of the test box body (1) and is fixedly connected with a crank (10), the other end of the transmission shaft (9) is fixedly connected with a bearing piece, the bearing piece is correspondingly arranged with the annular chute (8), a plurality of observation box placement frames (11) are arranged on the bearing piece, an observation box is detachably connected to the observation box placement frames (11), and the observation boxes are correspondingly arranged with the through holes (5);
the bearing piece comprises a bearing disc (12) fixedly connected to the transmission shaft (9), the center point of the bearing disc (12) is coaxially arranged with the center point of the disc (7), six supporting rods (13) are fixedly connected to the outer wall of the bearing disc (12) at equal intervals along the circumferential direction, one end of each supporting rod (13) away from the bearing disc (12) is rotationally connected with a supporting shaft (14), an observation box placement frame (11) is fixedly connected to one end of each supporting shaft (14), one end of each supporting shaft (14) is fixedly connected with a connecting plate (15), the other end of each connecting plate (15) is hinged to one end of each connecting plate (16), the sliding blocks (16) are matched with the annular sliding grooves (8), connecting rods (33) are arranged on the observation box placement frame (11), and two ends of each connecting rod (33) are hinged to two adjacent observation box placement frames (11).
2. The grain storage insect hypoxia stress test device according to claim 1, wherein: the novel portable observation box is characterized in that the top end of the observation box is detachably connected with a box cover (17), the bottom end of the observation box is slidably connected with a bottom plate (18), and a plurality of vent holes are formed in the box cover (17), the bottom plate (18) and the side wall of the observation box.
3. The grain storage insect hypoxia stress test device according to claim 1, wherein: the humidity control mechanism comprises a humidity control box (19) fixedly connected to the inner wall of the processing cavity (3), a water injection hole (20) is formed in the outer side wall of the test box body (1), the humidity control box (19) is communicated with the water injection hole (20), and a sealing plug (21) is arranged on the water injection hole (20).
4. The grain storage insect hypoxia stress test device according to claim 1, wherein: the gas control mechanism comprises a gas inlet (22) and a gas outlet (23) which are formed in the outer side wall of the test box body (1), the gas inlet (22) and the gas outlet (23) are communicated with the treatment cavity (3), the gas inlet (22) is fixedly connected with an external gas cylinder (24) through a pipeline, the gas outlet (23) is fixedly connected with a gas concentration detector (25) through a pipeline, and gas switch valves (26) are arranged on the gas inlet (22) and the gas outlet (23).
5. The grain storage insect hypoxia stress test device according to claim 4, wherein: the pressure control mechanism comprises a U-shaped pressure gauge (27) fixedly connected to the test box body (1), one end of the U-shaped pressure gauge (27) is communicated with the air outlet (23) through a pipeline, and the other end of the U-shaped pressure gauge (27) is open.
6. The grain storage insect hypoxia stress test device according to claim 1, wherein: the compressing mechanism comprises two fixing seats (28) fixedly connected to the front end of the box cover (6), two hooks (29) are fixedly connected to the front end of the test box body (1), and the hooks (29) are matched with the fixing seats (28).
7. The grain storage insect hypoxia stress test device according to claim 2, wherein: two holes (30) are formed in the front end of the test box body (1), the holes (30) are communicated with the operation cavity (4), sealing gloves are fixedly connected to the holes (30), threaded openings (31) are formed in the outer side wall of the test box body (1), the threaded openings (31) are communicated with the operation cavity (4), and threaded cover plates (32) are connected to the threaded openings (31) in a threaded mode.
8. A method for testing hypoxia stress of stored-grain insects, which is suitable for the hypoxia stress testing device of stored-grain insects according to claim 1, and is characterized in that: the method comprises the following steps:
a. placing insects to be tested: injecting clear water or saturated saline water into the humidity control mechanism, and placing insects with consistent growth states and numbers into the insect observation mechanism;
b. adjusting the gas concentration: regulating the gas concentration in the processing cavity (3) through a gas control mechanism;
c. and (3) performing sealing observation: c, volatilizing clear water or saturated saline water in the step a to a target value, and sealing the test box body (1) for observation;
d. adjusting the pressure: the internal pressure of the test box body (1) is regulated by a pressure control mechanism;
e. taking out a sample and observing: insect samples were taken from the test chamber (1) and observed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210486115.0A CN114794025B (en) | 2022-05-06 | 2022-05-06 | Device and method for testing hypoxia stress of stored-grain insects |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210486115.0A CN114794025B (en) | 2022-05-06 | 2022-05-06 | Device and method for testing hypoxia stress of stored-grain insects |
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| Publication Number | Publication Date |
|---|---|
| CN114794025A CN114794025A (en) | 2022-07-29 |
| CN114794025B true CN114794025B (en) | 2023-08-29 |
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| CN202210486115.0A Active CN114794025B (en) | 2022-05-06 | 2022-05-06 | Device and method for testing hypoxia stress of stored-grain insects |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN204594963U (en) * | 2015-05-08 | 2015-08-26 | 河南工业大学 | Circulating carbon dioxide detector |
| CN205093389U (en) * | 2015-10-10 | 2016-03-23 | 华南农业大学 | Observe simple and easy device that stores up grain pest horizontal proliferation activity |
| CN106258633A (en) * | 2016-10-21 | 2017-01-04 | 山西大学 | A kind of animals and plants incubator of real-time Simulation wild environment |
| CN109490364A (en) * | 2018-12-29 | 2019-03-19 | 河南工业大学 | A kind of peanut pod water transport variation test device |
| CN209931261U (en) * | 2019-05-29 | 2020-01-14 | 河南工业大学 | Device for observing motion behaviors of stored grain insects |
| CN211832469U (en) * | 2020-03-19 | 2020-11-03 | 山东省蚕业研究所 | Black soldier fly egg incubator |
| CN216314924U (en) * | 2021-10-25 | 2022-04-19 | 王健力 | Biological information observation incubator for insects |
-
2022
- 2022-05-06 CN CN202210486115.0A patent/CN114794025B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN204594963U (en) * | 2015-05-08 | 2015-08-26 | 河南工业大学 | Circulating carbon dioxide detector |
| CN205093389U (en) * | 2015-10-10 | 2016-03-23 | 华南农业大学 | Observe simple and easy device that stores up grain pest horizontal proliferation activity |
| CN106258633A (en) * | 2016-10-21 | 2017-01-04 | 山西大学 | A kind of animals and plants incubator of real-time Simulation wild environment |
| CN109490364A (en) * | 2018-12-29 | 2019-03-19 | 河南工业大学 | A kind of peanut pod water transport variation test device |
| CN209931261U (en) * | 2019-05-29 | 2020-01-14 | 河南工业大学 | Device for observing motion behaviors of stored grain insects |
| CN211832469U (en) * | 2020-03-19 | 2020-11-03 | 山东省蚕业研究所 | Black soldier fly egg incubator |
| CN216314924U (en) * | 2021-10-25 | 2022-04-19 | 王健力 | Biological information observation incubator for insects |
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| CN114794025A (en) | 2022-07-29 |
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