CN114790426A - Application of Desmodium hirsutum strain in production of gamma aminobutyric acid and cultivation method thereof - Google Patents
Application of Desmodium hirsutum strain in production of gamma aminobutyric acid and cultivation method thereof Download PDFInfo
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Abstract
The invention discloses an application of a Curculregion congguls strain in the production of gamma-aminobutyric acid and a cultivation method thereof, wherein the Curculregion congguls strain is named as Fv-HL.
Description
Technical Field
The invention belongs to the technical field of breeding of a eurotium cristatum strain, and particularly relates to application of the eurotium cristatum strain in production of gamma aminobutyric acid and a culture method thereof.
Background
Gamma-aminobutyric acid (GABA) is a naturally occurring four-carbon non-protein amino acid, widely distributed in nature. Researches prove that GABA is a main amino acid neurotransmitter involved in inhibitory synaptic transmission, and has wide physiological functions such as blood pressure reduction, blood sugar reduction, arrhythmia regulation, anxiety resistance, liver protection and the like. In human brain, GABA is converted from brain glutamate under the action of glutamate decarboxylase with strong specificity. But with the increase of age and mental stress, the accumulation of GABA becomes abnormally difficult. The health of human body can be improved by supplementing daily diet.
GABA content is found and measured in almost all important commercial and model crops except bacteria, fungi and animals. The GABA of common crops such as tomatoes, spinach, broccoli and the like is 300-400 mg/kg, and the GABA content in wheat germs is accumulated to 1630 mg/kg. GABA in edible fungi is less studied.
At present, common GABA preparation methods include a chemical synthesis method, a plant enrichment method and a microbial fermentation method. The chemical synthesis method has the defects of high cost, low yield, harsh reaction conditions, easy chemical component residue, easy environmental pollution and the like, and is not suitable for the aspects of food, medicines and the like. The plant enrichment method has high cost, low yield and better safety; the microbial fermentation method has low cost and high yield, and is not limited by space, environment and resources, so the plant enrichment method and the microbial fermentation method are more selected to enrich GABA. At present, no literature report exists on a breeding method of high-yield gamma-aminobutyric acid malpighia and the content of gamma-aminobutyric acid enriched by utilizing a microbial fermentation method.
Disclosure of Invention
This section is for the purpose of summarizing some aspects of embodiments of the invention and to briefly introduce some preferred embodiments. In this section, as well as in the abstract and title of the application, simplifications or omissions may be made to avoid obscuring the purpose of the section, the abstract and the title, and such simplifications or omissions are not intended to limit the scope of the invention.
As one aspect of the invention, the invention provides an application of a Collybia bacteria strain in the production of gamma aminobutyric acid, which is characterized in that: the strain name of the Desmodium hirsutum strain is Fv-HL.
As another aspect of the present invention, the present invention provides the cultivation method of the Collybia bacteria species, which comprises the following steps:
(1) inoculating the desmodium hirsutum strain to a potato dextrose agar solid culture medium PDA, and collecting hyphae after culturing;
(2) selecting the desmodium hirsutum cultured in the step (1), inoculating the desmodium hirsutum into a potato glucose broth liquid culture medium PDB, and culturing in a dark place to obtain the mycelial of the desmodium hirsutum;
(3) inoculating the hypha obtained in the step (1) into a sterile culture bottle for culture, performing fruiting management, and culturing in a dark place to obtain the monilia peduncularis sporocarp.
As a preferred scheme of the cultivation method of the desmodium hirsutum strain, the method comprises the following steps: the potato glucose agar solid culture medium PDA comprises 3.9-4.5% of potato glucose agar by mass.
As a preferred scheme of the cultivation method of the Tricholoma hirsutum strain, the method comprises the following steps: the weight percentage content of the potato dextrose broth liquid culture medium PDB is 2.4-3.0%.
As a preferred scheme of the cultivation method of the Tricholoma hirsutum strain, the method comprises the following steps: in the step (1), hyphae are collected after the cultivation, and the cultivation time is 10-12 days.
As a preferred scheme of the cultivation method of the desmodium hirsutum strain, the method comprises the following steps: in the step (1), hyphae are collected after the culture, and the culture temperature is 22-25 ℃.
As a preferred scheme of the cultivation method of the Tricholoma hirsutum strain, the method comprises the following steps: in the step (2), the light-tight culture is carried out at 22-25 ℃ for 10-12 days, and then hyphae are collected.
As a preferred scheme of the cultivation method of the Tricholoma hirsutum strain, the method comprises the following steps: in the step (3), the light-shielding culture is carried out for 50-70 days at 25 ℃.
As a preferred scheme of the cultivation method of the Tricholoma hirsutum strain, the method comprises the following steps: in the step (2), 0.5-1 g/L of L-glutamic acid is added into the potato glucose broth liquid culture medium PDB.
As a preferred scheme of the cultivation method of the desmodium hirsutum strain, the method comprises the following steps: in the step (3), 2.4-3.2 g/L of L-glutamic acid is added into the cultivation bottle.
The invention has the beneficial effects that: the obtained high-yield strain Fv-HL of the aureobasidium hirsutum gamma-aminobutyric acid has the GABA content in mycelium of 28431.47mg/kg (figure 4) and the GABA content in fruiting body of 4303.63mg/kg (figure 5).
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In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required to be used in the description of the embodiments are briefly introduced below, and it is obvious that the drawings in the description below are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without inventive labor. Wherein:
FIG. 1 is a morphological diagram of mycelium and fruiting body of Lysimachia pilifera.
FIG. 2 is the change in GABA content during the growth of C.hirsutum.
FIG. 3 is a graph of the different growth phases of the proton entities of the species Desmodium hirsutum.
FIG. 4 is a peak diagram showing GABA content measured by culturing the Fv-HL mycelium of the C.hirsutum strain.
FIG. 5 is a peak diagram of GABA content obtained by culturing and detecting the Fv-HL fruit body of the Lysimachia christinae strain.
Detailed Description
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with examples are described in detail below.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, but the present invention may be practiced in other ways than those specifically described and will be readily apparent to those of ordinary skill in the art without departing from the spirit of the present invention, and therefore the present invention is not limited to the specific embodiments disclosed below.
Furthermore, the references herein to "one embodiment" or "an embodiment" refer to a particular feature, structure, or characteristic that may be included in at least one implementation of the present invention. The appearances of the phrase "in one embodiment" in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments.
Example 1:
the main reagents and culture medium are configured as follows:
PDA solid medium: potato dextrose agar powder 39g, 1000mL distilled water, natural pH, used for hypha culture.
PDB liquid medium: potato dextrose broth powder 24g, 1000ml distilled water, natural pH, used for hypha culture.
Seed liquid culture medium: 20g of white granulated sugar, 3.3g of puffed soybean meal, 0.66g of magnesium sulfate, 0.51g of monopotassium phosphate, 0.15g of dipotassium phosphate, and double distilled water with the constant volume of 1L, wherein the pH value is adjusted to 5.8-6.2, and the mixture is used for hypha culture.
0.1M hydrochloric acid: 860 mu L of hydrochloric acid is added with distilled water to be constant volume to 100mL, and the mixture is stored in a sealed container after being mixed evenly.
10% trichloroacetic acid solution: 10g of trichloroacetic acid is weighed, dissolved in 100mL of distilled water, mixed uniformly and stored in a sealed container.
Amino acid extracting solution: preparing a solution from 0.1M hydrochloric acid and a 10% trichloroacetic acid solution according to a volume ratio of 1:2, uniformly mixing, sealing and storing in a dark place.
In order to explore the difference of GABA content of different strains of Desmodium hirsutum, 26 factory production strains are screened from a germplasm resource library for cultivation test, wherein the serial numbers are respectively Fv-WC, ENOKI-J, J54-3, x 3E, J5011, WH25, SCY1-2, Fv-SY, ENOKI-H, WX01, ENOKI-I, W1638, XH, FM, Fv-FM, 2345, Fv-GF, Fv-RYJ, Fv-KL, Fv-BY, Fv-HTC, ENOKI-G, Fv-GR, Fv-CYS, Fv-YH and Fv-HL, as shown in figure 1. Finally, 22 strains successfully grow. FIG. 1 a shows the growth pattern of the mycelium of C.hirsutum, and FIG. 1 b shows the growth pattern of the sporophore of C.hirsutum.
The cultivating and detecting method of the hirsutella sinensis comprises the following steps:
(1) inoculating the strain of the desmodium hirsutum into a Potato Dextrose Agar (PDA) solid culture medium, culturing at 25 ℃ for 10 days, and collecting hyphae.
(2) Picking 10 blocks of 8mm cultured on the PDA plate in the step (1) 2 The Tricholoma hirsutum (Fr.) Sing is inoculated into potato glucose broth liquid culture medium (PDB), and cultured at 25 deg.C in dark for 10 days to collect mycelium.
(3) Accurately weighing 1g of mycelium obtained in the step (2) after filtration, adding 5mL of amino acid extracting solution (preparing solution by 0.1M hydrochloric acid and 10% trichloroacetic acid solution according to the volume ratio of 1: 2), carrying out ice bath ultrasound for 10min, wherein the working condition is that the ultrasonic power is 200W, the working time is 3.0s, and the time is 3.0 s. Centrifuging at 8000rpm at 4 deg.C for 10min after the ultrasonic treatment is completed, collecting supernatant, storing in-20 deg.C refrigerator, and measuring gamma-aminobutyric acid content with Hitachi 835-50 type amino acid automatic analyzer.
(4) And (3) plating the activated monilia peduncularis in the step (1) into a sterile cultivation bottle for cultivation, and performing fruiting management. Culturing at 25 deg.C in dark for 50-70 days to obtain fresh Lysimachia pedunculata fruiting body.
(5) Weighing 150g of fresh monilia peduncularis fruiting body in mature period, placing at 55 deg.C, and hot air drying to constant weight. Crushing by a high-speed crusher, sieving by a 60-mesh sieve, and sealing and storing sample powder in a cool and dry place.
(6) 70-80mg of sample powder to be measured is accurately weighed, 6mol/L hydrochloric acid solution is added for acid hydrolysis, and the content of gamma-aminobutyric acid is measured by a Hitachi 835-50 type amino acid automatic analyzer.
The GABA content (DW) of 22 M.hirsutella sporophores was determined by amino acid analyzer, and the GABA content of M.hirsutella was significantly different between different varieties, as shown in Table 1.
Table 122 GABA content (DW) of Lysimachia pilotiana sporophore
Spatiotemporal variation of GABA content during fruiting body development: the GABA content of the stipe and pileus of the monilia pedunculata in different growth periods is determined by an amino acid analyzer, and the result is shown in figure 2, and the GABA content in the stipe and pileus of the monilia pedunculata sporocarp shows a trend of descending firstly and then ascending secondly, and the GABA content accumulation reaches the maximum value in the third stage. During the whole fruiting body development process, whether in high-yield or low-yield GABA strains, GABA content in pileus is higher than GABA content in stipe. Wherein the GABA content in the fruiting body pileus of Fv-HL mature period is up to 5056.88mg/kg DW, which is about 1.9 times of that of Fv-HL stipe at the same period. FIG. 3 shows different growth periods of the proton entity of Desmodium hirsutum, FIGS. 3 (a) - (c) are respectively the first stage, the second stage and the third stage of the growth of Desmodium hirsutum, 1 and 2 represent Desmodium hirsutum proton Enoki-J, Fv-HL respectively.
The Fv-HL strain is a disclosed strain which is described in the core germplasm group and the molecular identity card for constructing the flammulina velutipes based on genetic diversity, the journal of bacteriology, the Gaolihui and the like, 2021.
Example 2:
(1) inoculating Lysimachia hirsuta Fv-HL strain to potato dextrose agar solid medium (PDA), culturing at 25 deg.C for 10d, and collecting mycelium.
(2) Picking 10 blocks of 8mm cultured on the PDA plate in the step (1) 2 The myceliophthora hirsutella mass is inoculated in potato glucose broth liquid culture medium (PDB), and is cultured in the dark at 25 ℃ for 10 days, and then hyphae are collected.
(3) Accurately weighing 1g of mycelium obtained in the step (2) after filtration, adding 5mL of amino acid extracting solution (preparing solution by 0.1M hydrochloric acid and 10% trichloroacetic acid solution according to the volume ratio of 1: 2), carrying out ice bath ultrasound for 10min, wherein the working condition is that the ultrasonic power is 200W, the working time is 3.0s, and the time is 3.0 s. Centrifuging at 8000rpm for 10min at 4 deg.C after the ultrasonic treatment, collecting supernatant, storing in a refrigerator at-20 deg.C, and measuring gamma-aminobutyric acid content with Hitachi 835-50 type amino acid automatic analyzer.
(4) And (2) plating the activated monitopsis hirsutella in the step (1) into a sterile culture bottle for culture, and performing fruiting management. Culturing at 25 deg.C in dark for 50-70 days to obtain fresh Lysimachia christinae Hance fruiting body.
(5) Weighing 150g of fresh monilia peduncularis fruiting body in mature period, placing at 55 deg.C, and hot air drying to constant weight. Crushing by a high-speed crusher, sieving by a 60-mesh sieve, and sealing and storing sample powder in a cool and dry place.
(6) 70-80mg of sample powder to be measured is accurately weighed, 6mol/L hydrochloric acid solution is added for acid hydrolysis, and the content of gamma-aminobutyric acid is measured by a Hitachi 835-50 type amino acid automatic analyzer.
And (4) counting results: amino acid analyzer determined GABA content obtained by culturing Fv-HL mycelium of Desmodium hirsutum strain 28431.47mg/kg (figure 4), GABA content obtained by culturing fruiting body 4303.63mg/kg dry weight (figure 5), and GABA content obtained by culturing fruiting body 645.54mg/kg fresh weight.
Study example:
comparing different strains, respectively carrying out laboratory liquid culture in culture media with different components, and determining GABA content in different culture media. GABA content data showed that the GABA content obtained by culturing the mycelia in the seed broth was 1018.72mg/kg, whereas the GABA content obtained by culturing the mycelia in the PDB broth was 28431.47mg/kg, which is much higher than that of the seed broth. Therefore, PDB medium was selected as the laboratory mycelium liquid medium.
The method is characterized in that L-glutamic acid is added into a PDB culture medium, and the influence of the L-glutamic acid on the GABA content of hyphae is observed, and the result shows that the GABA producing capability of a strain can be improved by adding 0.5-1 g/L of low-concentration L-glutamic acid, and the GABA synthesis of the mycelial bodies of the Desmodium hirsutum is inhibited by adding high-concentration glutamic acid. When the glutamic acid addition concentration was 0.5g/L, the GABA content in mycelia was the highest, which was 41.9% higher than that in the control group (example 2, using PDB medium without addition of nutrients). However, when L-glutamic acid was added at a concentration of 1.5g/L, GABA production in the mycelia was lower than that of the control group. The GABA enriching ability of the mycelium is reduced on the contrary along with the continuous increase of the adding concentration of the L-glutamic acid, which is probably because the GABA enriching ability of the mycelium is limited by utilizing the L-glutamic acid to carry out biotransformation, and the osmotic pressure of cell membranes is enhanced by high concentration of a substrate, so that the growth of thalli is inhibited, and the accumulation of GABA is not facilitated.
The L-glutamic acid is added into the solid culture medium of the Desmodium hirsutum culture bottle, the influence of the L-glutamic acid on the GABA content of the sporophore level is observed by taking the solid culture medium without the L-glutamic acid as a control, and the result shows that when the addition concentration of the L-glutamic acid is 3.2g/L, the GABA enriched yield of the Desmodium hirsutum sporophore reaches the maximum and is 1.95 times of that of the control group. The GABA yield is reduced instead of increasing continuously because the GABA synthesizing capacity of GAD is limited in the GABA synthesizing path, and the excessive high concentration of L-glutamic acid may saturate the L-glutamic acid in the fruiting body and can not promote the GABA decarboxylation enriching reaction continuously.
It should be noted that the above-mentioned embodiments are only for illustrating the technical solutions of the present invention and not for limiting, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions may be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, which should be covered by the claims of the present invention.
Claims (10)
1. The application of the Desmodium hirsutum strain in the production of gamma aminobutyric acid is characterized in that: the strain name of the Desmodium hirsutum strain is Fv-HL.
2. A method of cultivating a species of Lysimachia capillipes according to claim 1, characterized in that: the method comprises the following steps:
(1) inoculating the desmodium hirsutum strain to a potato dextrose agar solid culture medium PDA, and collecting hyphae after culturing;
(2) selecting the desmodium hirsutum cultured in the step (1), inoculating the desmodium hirsutum into a potato glucose broth liquid culture medium PDB, and culturing in a dark place to obtain the mycelial of the desmodium hirsutum;
(3) inoculating the hyphae obtained in the step (1) into a sterile cultivation bottle for cultivation, performing fruiting management, and performing light-resistant cultivation to obtain the stipe demersal fungus sporocarp.
3. A method of cultivating a species of Collybia pilosellus according to claim 2, characterized in that: the potato glucose agar solid culture medium PDA comprises 3.9-4.5% of potato glucose agar by mass.
4. A method of cultivating a species of Collybia pilosellus according to claim 2 or 3, characterized in that: the weight percentage content of the potato dextrose broth liquid culture medium PDB is 2.4-3.0%.
5. A method of cultivating a species of Collybia trichinae as claimed in claim 2 or 3, characterized in that: in the step (1), hyphae are collected after the culture, and the culture time is 10-12 days.
6. A method of cultivating a species of Collybia pilosellus according to claim 2 or 3, characterized in that: in the step (1), hyphae are collected after the culture, and the culture temperature is 22-25 ℃.
7. A method of cultivating a species of Collybia pilosellus according to claim 2 or 3, characterized in that: and (3) in the step (2), performing light-shielding culture at 22-25 ℃ for 10-12 days, and collecting hyphae.
8. A method of cultivating a species of Collybia pilosellus according to claim 2 or 3, characterized in that: in the step (3), the light-shielding culture is carried out for 50-70 days at 25 ℃.
9. A method of cultivating a species of Collybia pilosellus according to claim 2 or 3, characterized in that: in the step (2), 0.5-1 g/L of L-glutamic acid is added into the potato glucose broth liquid culture medium PDB.
10. A method of cultivating a species of Collybia pilosellus according to claim 2 or 3, characterized in that: in the step (3), 2.4-3.2 g/L of L-glutamic acid is added into the cultivation bottle.
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009039082A (en) * | 2007-08-13 | 2009-02-26 | Acera:Kk | METHOD FOR PRODUCING MUSHROOM CONTAINING MUCH gamma-AMINOBUTYRIC ACID, MUSHROOM CONTAINING MUCH gamma-AMINOBUTYRIC ACID, FOOD RAW MATERIAL CONTAINING MUCH gamma-AMINOBUTYRIC ACID AND FOODSTUFF ENRICHED WITH gamma-AMINOBUTYRIC ACID |
| JP2012187068A (en) * | 2011-03-11 | 2012-10-04 | Hokkaido Research Organization | γ-AMINO BUTYRIC ACID-CONTAINING FOOD, AND METHOD FOR PRODUCING THE SAME |
| CN102986536A (en) * | 2012-12-02 | 2013-03-27 | 中华全国供销合作总社昆明食用菌研究所 | Flammulina velutipes strain and preparation method |
| KR20170024370A (en) * | 2015-08-25 | 2017-03-07 | 농업회사법인 주식회사 생생초 | Lactic acid fermentation broth containing GABA method of producing components using the mushroom extract |
| CN108719764A (en) * | 2018-05-23 | 2018-11-02 | 广东医科大学 | A kind of production method of compound bio enzyme process high-content GABA functionality rice flour |
| CN112941223A (en) * | 2021-03-15 | 2021-06-11 | 上海市农业科学院 | Golden needle mushroom 6168 strain and identification method and construction method and application of SSR marker fingerprint spectrum thereof |
| CN113186327A (en) * | 2021-04-08 | 2021-07-30 | 上海市农业科学院 | Identification method of microsatellite DNA marker fingerprint of flammulina velutipes FC89 strain, construction method and application thereof |
| CN113229494A (en) * | 2021-04-14 | 2021-08-10 | 四川省农业科学院土壤肥料研究所 | Needle mushroom water extract with high gamma-aminobutyric acid content and preparation method thereof |
-
2022
- 2022-05-19 CN CN202210554434.0A patent/CN114790426B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009039082A (en) * | 2007-08-13 | 2009-02-26 | Acera:Kk | METHOD FOR PRODUCING MUSHROOM CONTAINING MUCH gamma-AMINOBUTYRIC ACID, MUSHROOM CONTAINING MUCH gamma-AMINOBUTYRIC ACID, FOOD RAW MATERIAL CONTAINING MUCH gamma-AMINOBUTYRIC ACID AND FOODSTUFF ENRICHED WITH gamma-AMINOBUTYRIC ACID |
| JP2012187068A (en) * | 2011-03-11 | 2012-10-04 | Hokkaido Research Organization | γ-AMINO BUTYRIC ACID-CONTAINING FOOD, AND METHOD FOR PRODUCING THE SAME |
| CN102986536A (en) * | 2012-12-02 | 2013-03-27 | 中华全国供销合作总社昆明食用菌研究所 | Flammulina velutipes strain and preparation method |
| KR20170024370A (en) * | 2015-08-25 | 2017-03-07 | 농업회사법인 주식회사 생생초 | Lactic acid fermentation broth containing GABA method of producing components using the mushroom extract |
| CN108719764A (en) * | 2018-05-23 | 2018-11-02 | 广东医科大学 | A kind of production method of compound bio enzyme process high-content GABA functionality rice flour |
| CN112941223A (en) * | 2021-03-15 | 2021-06-11 | 上海市农业科学院 | Golden needle mushroom 6168 strain and identification method and construction method and application of SSR marker fingerprint spectrum thereof |
| CN113186327A (en) * | 2021-04-08 | 2021-07-30 | 上海市农业科学院 | Identification method of microsatellite DNA marker fingerprint of flammulina velutipes FC89 strain, construction method and application thereof |
| CN113229494A (en) * | 2021-04-14 | 2021-08-10 | 四川省农业科学院土壤肥料研究所 | Needle mushroom water extract with high gamma-aminobutyric acid content and preparation method thereof |
Non-Patent Citations (4)
| Title |
|---|
| AKIRA HARADA等: "Production of GABA-Enriched Powder by a Brown Variety of Flammulina velutipes (Enokitake) and Its Antihypertensive Effects in Spontaneously Hypertensive Rats", 《NIPPON SHOKUHIN KAGAKU KOGAKU KAISHI》 * |
| SHIN-YU CHEN AND KUNG-JUI HO AND YUN-JUNG HSIEH AND LI-TING WANG AND JENG-LEUN MAU: "Contents of lovastatin, γ-aminobutyric acid and ergothioneine in mushroom fruiting bodies and mycelia", 《LWT - FOOD SCIENCE & TECHNOLOGY》 * |
| 方芳等: "大豆γ-氨基丁酸的富集及产品开发研究进展", 《食品工业科技》 * |
| 高士友等: "毛柄金钱菌优质高产栽培技术的研究", 《陕西农业科学》 * |
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| CN114790426B (en) | 2023-06-06 |
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