CN114789027A - Simple methane gas preparation and collection device and method for laboratory - Google Patents
Simple methane gas preparation and collection device and method for laboratory Download PDFInfo
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- CN114789027A CN114789027A CN202210493639.2A CN202210493639A CN114789027A CN 114789027 A CN114789027 A CN 114789027A CN 202210493639 A CN202210493639 A CN 202210493639A CN 114789027 A CN114789027 A CN 114789027A
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J7/00—Apparatus for generating gases
- B01J7/02—Apparatus for generating gases by wet methods
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
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- B01L5/02—Gas collection apparatus, e.g. by bubbling under water
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Abstract
The invention belongs to the technical field of laboratory gas preparation, and discloses a simple methane gas preparation and collection device and method for a laboratory, wherein the simple methane gas preparation and collection device for the laboratory is provided with: a gas preparation structure for generating gas through the reaction of a liquid experimental sample in the conical flask; the gas transition structure realizes the flow transition of gas through the two glass guide pipes and the elastic rubber pipe; and the gas collecting structure collects gas in the sample by a drainage and gas collection method through the vacuum aluminum foil gas bag. The gas preparation structure is provided with a conical flask and a silica gel plug, and the silica gel plug is plugged in the mouth of the conical flask. The invention is used for researching the concentration change of gas such as methane generated by collecting a bacteria liquid sample in a laboratory. The invention can realize the self-control of the gas preparation and collection device by utilizing conventional laboratory equipment, has simple operation and low cost, and can effectively realize the preparation and collection of the required gas sample.
Description
Technical Field
The invention belongs to the technical field of laboratory gas preparation, and particularly relates to a simple methane gas preparation and collection device and method for a laboratory.
Background
Microorganisms can act on certain specific metabolic substrates such as methylphosphonic acid and the like to produce methane gas. Methane is an important greenhouse gas in the atmosphere, has 28 times of greenhouse effect as carbon dioxide, can directly or indirectly affect the global climate, and contributes to the global warming by about 20 percent. The research on the concentration and the generation mechanism of the methane gas is of great significance for the research on the greenhouse effect and the global climate change condition. In order to detect the concentration of methane gas, it is a necessary prerequisite to collect the gas generated in the bacterial liquid sample.
A conventional laboratory gas preparation apparatus includes: a solid-liquid normal temperature device, a solid-liquid heating device, a solid-solid heating device and the like. Methods of laboratory gas collection include an upward air evacuation method, a downward air evacuation method, and a water evacuation method. The above apparatus and method are liable to cause leakage or contamination of the sample gas and it is difficult to control the content of the collected gas.
The traditional gas collecting method and equipment comprise the steps of liquefying or adsorbing and pressurizing gas by using a gas storage tank, an adsorption box and the like for collection, are not easy to operate, have overlarge specifications, are high in cost and are complicated in manufacturing and inspection processes.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a simple methane gas preparation and collection device and method for a laboratory.
The invention is realized in such a way that a laboratory is with simple and easy methane gas preparation and collection device, the laboratory is with simple and easy methane gas preparation and collection device is provided with:
a gas preparation structure for generating gas through the reaction of a liquid experimental sample in the conical flask;
the gas transition structure realizes the flow transition of gas through the two glass guide pipes and the elastic rubber pipe;
and collecting the gas in the sample by a drainage and gas collection method through a vacuum aluminum foil air bag.
Further, the gas preparation structure is provided with a conical flask and a silica gel plug, and the silica gel plug is plugged in the mouth of the conical flask.
Further, the conical flask is a 250mL conical flask; the silica gel stopper is a diplopore silica gel stopper, and the external diameter is 42mm, internal diameter 32mm, and high 30mm, diplopore aperture is 7 mm.
Further, the gas transition structure is provided with a first glass guide pipe, a first elastic rubber pipe, a water stop clamp, a second glass guide pipe and a second elastic rubber pipe;
the first glass guide pipe and the second glass guide pipe are plugged in the silica gel plug, the first glass guide pipe is connected with the first elastic rubber pipe, the end part of the first elastic rubber pipe is provided with a water stop clamp, and the second glass guide pipe is connected with the second elastic rubber pipe.
Further, the first glass conduit is an L-shaped right-angle elbow with the size of 5 x 15 cm; the lower end of the first glass conduit extends to a position below the liquid level of the solution sample and above the bottom of the conical flask.
Further, the second glass conduit is an L-shaped right-angle elbow with the size of 5 x 5 cm.
Further, the inner diameter of the first elastic rubber tube is 5mm, and the outer diameter of the first elastic rubber tube is 7 mm.
Furthermore, the gas collecting structure is provided with an adapter, a vacuum aluminum foil air bag and an injector, the adapter is connected with the second elastic rubber tube, and the adapter is installed on the vacuum aluminum foil air bag.
Further, the syringe is a 50mL syringe, the vacuum aluminum foil air bag is 12 x 14cm in size, and the adapter is a single valve switch.
Another object of the present invention is to provide a laboratory simple gas preparing and collecting method using the laboratory simple methane gas preparing and collecting apparatus, the laboratory simple gas preparing and collecting method including:
the liquid experimental sample is placed at the bottom of the conical flask, and the volume is 50 mL; after the bottle mouth is blocked by the silica gel plug, sealing and reinforcing the joint of the bottle mouth by using a sealing film in a laboratory;
a first glass conduit and a second glass conduit in the gas transition structure are dipped with a small amount of ultrapure water and then are plugged into the silica gel plug double holes, and then elastic rubber tubes with proper lengths are respectively connected;
a first elastic rubber tube connected with a first glass guide tube in the gas collecting structure is clamped by a water stop clamp in the gas preparation process, and the water stop clamp is opened in the gas collecting process;
the second glass catheter is connected with the adapter through a second elastic rubber tube and then connected with the injector, saturated saline is injected, the injection is carried out for 4 times, 200mL of liquid is counted in total, and gas in 200mL of samples is collected by a drainage and gas collection method; after the gas is collected, the adapter is closed, and the opening of the adapter is tightly wrapped and sealed by a laboratory sealing film so as to prevent the gas from overflowing or other gases in the air from mixing into the polluted sample.
In combination with the technical solutions and the technical problems to be solved, please analyze the advantages and positive effects of the technical solutions to be protected in the present invention from the following aspects:
first, aiming at the technical problems existing in the prior art and the difficulty in solving the problems, the technical problems to be solved by the technical scheme of the present invention are closely combined with results, data and the like in the research and development process, and some creative technical effects are brought after the problems are solved. The specific description is as follows:
the invention can be used for collecting gas generated in a bacterial liquid sample in a laboratory, and further used for the concentration change research of methane gas generated by the action of microorganisms on special metabolic substrates such as methylphosphonic acid and the like. The invention can realize the self-control of the gas preparation and collection device by utilizing conventional laboratory equipment, has simple operation and low cost, and can effectively realize the preparation and collection of the required gas sample.
Secondly, considering the technical scheme as a whole or from the perspective of products, the technical effect and advantages of the technical scheme to be protected by the invention are specifically described as follows:
the simple gas preparation and collection device is manufactured by utilizing laboratory conventional conical bottles, gas collection bags, injectors and other experimental articles, can be used for gas preparation and collection experiments, provides help for subsequent gas detection and research, and is simple and convenient to operate, low in cost and simple and easy to obtain required equipment.
Thirdly, the inventive auxiliary proof as the claimed invention is also embodied in the following important aspects:
the expected income and commercial value after the technical scheme of the invention is converted are as follows: the related instruments and equipment of the technical scheme can be assembled to form a complete device for preparing and collecting gas in a laboratory, and the device can be commercially sold in batch production.
The technical scheme of the invention solves the technical problem that people are eager to solve but can not succeed all the time: the problem of laboratory gas difficult collection is solved, the preparation is collected integratively, and the whole is sealed, and sample gas is difficult for leaking or polluting, and it is convenient to collect, can supply further experimental research.
The technical scheme of the invention overcomes the technical prejudice whether: the method overcomes the prejudice of the traditional technology for generating methane in a laboratory, and can be generated by the aerobic action of microorganisms on methyl phosphonic acid besides chemical reactions such as heating; the device overcomes the defects of the traditional technology bias of laboratory materials such as conical bottles, syringes, water-stop clamps and the like, and can be used for preparing and collecting laboratory gases through an assembly technology.
Drawings
FIG. 1 is a schematic structural diagram of a simple methane gas preparing and collecting device for a laboratory according to an embodiment of the present invention;
FIG. 2 is a schematic diagram of a gas preparation structure provided by an embodiment of the present invention;
FIG. 3 is a schematic view of a gas transition structure provided by an embodiment of the present invention;
FIG. 4 is a schematic view of a gas collection configuration provided by an embodiment of the present invention;
in the figure: 1. a conical flask; 2. a first glass conduit; 3. a silica gel plug; 4. a water stop clip; 5. a first elastic rubber tube; 6. a second glass conduit; 7. a second elastic rubber tube; 8. an adapter; 9. vacuum aluminum foil air bag.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
First, an embodiment is explained. This section is an illustrative example developed to explain the claims in order to enable those skilled in the art to fully understand how to implement the present invention.
As shown in fig. 1 to 4, the simple methane gas preparing and collecting device for a laboratory provided in an embodiment of the present invention includes:
and the gas preparation structure is used for generating gas through the reaction of the liquid experimental sample in the conical flask.
And the gas transition structure realizes gas circulation transition through the two glass guide pipes and the elastic rubber pipe, wherein the water stop clamp is used for controlling the gas circulation.
And the gas collection structure collects gas in the sample by a drainage and gas collection method through the vacuum aluminum foil air bag, the injector and the water stop clamp.
The gas preparation structure is provided with a conical flask 1 and a silica gel plug 3, and the silica gel plug 3 is plugged in the mouth of the conical flask 1.
The gas transition structure is provided with a first glass conduit 2, a first elastic rubber tube 5, a water stop clamp 4, a second glass conduit 6 and a second elastic rubber tube 7; the first glass guide tube 2 and the second glass guide tube 6 are plugged in the silica gel plug 3, the first glass guide tube 2 is connected with the first elastic rubber tube 5, the water stop clamp 4 is installed at the end part of the first elastic rubber tube 5, and the second glass guide tube 6 is connected with the second elastic rubber tube 7.
The gas collecting structure is provided with an adapter 8, a vacuum aluminum foil air bag 9 and an injector, the adapter 8 is connected with the second elastic rubber tube 7, and the adapter 8 is installed on the vacuum aluminum foil air bag 9.
Wherein the erlenmeyer flask 1 is a 250mL erlenmeyer flask of Shuniu; the silica gel plug 3 is a double-hole silica gel plug, the outer diameter is 42mm, the inner diameter is 32mm, the height is 30mm, and the hole diameters of the double holes are 7 mm; the syringe was a 50mL syringe. The first glass conduit 2 is an "L" shaped right angle bend with dimensions of 5 x 15 cm. The lower end of the first glass conduit 2 extends to a position below the liquid level of the solution sample and above the bottom of the conical flask; the second glass conduit 6 is an L-shaped right-angle elbow with the size of 5cm and the lower end slightly extending into the bottle mouth; the inner diameter of the first elastic rubber tube 5 is 5mm, and the outer diameter is 7 mm; the vacuum aluminum foil air bag 9 is an aluminum foil product, is opaque, has good light shading performance, and can prevent the change of gas properties which are easily influenced by light factors; the vacuum aluminum foil air bag 9 is 12 cm by 14cm in size and has a maximum collectable gas volume of 200 mL. The adapter 8 is a single valve switch and can be controlled in a rotating way. The adapter 8 is always in an open state in the gas preparation and collection process, so that the device is prevented from being damaged by large pressure change in the gas generation process, and the adapter 8 is closed after the gas is collected.
The working principle of the invention is as follows: the liquid experimental sample is placed at the bottom of the conical flask 1, and the volume is 50 mL. And after the bottle mouth is blocked by the rear silica gel plug 3, sealing and reinforcing the joint of the bottle mouth by using a sealing film in a laboratory. In the gas transition structure first glass pipe 2 and second glass pipe 6 dip in and get behind a small amount of ultrapure water fill in 3 diplopores of silica gel plug in, after each extend suitable elasticity rubber tube (first elasticity rubber tube 5 and second elasticity rubber tube), the effect of water makes to connect more smoothly and the fastness is better. The first elastic rubber tube 5 connected with the first glass conduit 2 in the gas collecting structure is clamped by the water stop clamp 4 in the gas preparation process, and the water stop clamp 4 is opened in the gas collecting process. The second glass conduit 6 is connected with the adapter 8 through the second elastic rubber tube 7 and then connected with the injector, saturated saline is injected into the injector, the injection is carried out for 4 times, and 200mL of liquid is counted, namely, the gas in 200mL of samples is collected by utilizing the principle of drainage and gas collection. After the gas is collected, the adapter 8 is closed, and a laboratory sealing film is used for tightly wrapping and sealing the opening of the adapter 8, so that the gas is prevented from overflowing or other gases in the air are prevented from being mixed into the polluted sample.
And II, application embodiment. In order to prove the creativity and the technical value of the technical scheme of the invention, the part is the application example of the technical scheme of the claims on specific products or related technologies.
The method is particularly applied to the preparation and collection experiments of methane gas in a laboratory and is used for further researching the concentration change condition of methane generated by the action of microorganisms on special metabolic substrates such as methyl phosphonic acid and the like; the method is used for collecting gas generated by a liquid bacterium liquid sample and further detecting and analyzing the proportion of each component of the gas.
And thirdly, evidence of relevant effects of the embodiment. The embodiment of the invention achieves some positive effects in the process of research and development or use, and has great advantages compared with the prior art, and the following contents are described by combining data, diagrams and the like in the test process.
The gas preparation and collection device utilizing the technology is applied to the preparation and collection experiment of gas in a bacterial liquid sample in a laboratory, and the change condition of the concentration of methane generated by the action of microorganisms on methyl phosphonic acid along with time and CO in the methane are further detected and researched 2 And NO 2 The composition varied.
The above description is only for the purpose of illustrating the present invention and the appended claims are not to be construed as limiting the scope of the invention, which is intended to cover all modifications, equivalents and improvements that are within the spirit and scope of the invention as defined by the appended claims.
Claims (10)
1. The utility model provides a laboratory is with simple and easy methane gas preparation and collection device which characterized in that, laboratory is with simple and easy methane gas preparation and collection device is provided with:
the gas preparation structure generates gas through the reaction of a liquid experimental sample in the conical flask;
the gas transition structure realizes gas circulation transition through the two glass guide pipes and the elastic rubber pipe, and the water stop clamp is used for controlling gas circulation;
and the gas collecting structure collects gas in the sample by a drainage and gas collection method through the vacuum aluminum foil air bag, the injector and the water stop clamp.
2. The simple methane gas preparing and collecting device for the laboratory according to claim 1, wherein the gas preparing structure is provided with a conical flask and a silica gel plug, and the silica gel plug is plugged in the mouth of the conical flask.
3. The laboratory simple methane gas producing and collecting device of claim 2, wherein the erlenmeyer flask is a 250mL erlenmeyer flask; the silica gel stopper is a double-hole silica gel stopper, the outer diameter is 42mm, the inner diameter is 32mm, the height is 30mm, and the diameter of the double-hole is 7 mm.
4. The laboratory simple methane gas preparation and collection device according to claim 1, wherein the gas transition structure is provided with a first glass conduit, a first elastic rubber tube, a water stop clip, a second glass conduit, and a second elastic rubber tube;
the first glass guide pipe and the second glass guide pipe are plugged in the silica gel plug, the first glass guide pipe is connected with the first elastic rubber pipe, the end part of the first elastic rubber pipe is provided with a water stop clamp, and the second glass guide pipe is connected with the second elastic rubber pipe.
5. The laboratory simple methane gas producing and collecting device of claim 4, wherein the first glass conduit is an L-shaped right angle elbow having dimensions of 5 x 15 cm; the lower end of the first glass conduit extends to a position below the liquid level of the solution sample and above the bottom of the conical flask.
6. The laboratory simple methane gas producing and collecting device of claim 4, wherein the second glass conduit is an L-shaped right angle elbow having dimensions of 5 x 5 cm.
7. The laboratory simple methane gas producing and collecting device according to claim 4, wherein the first resilient rubber tube has an inner diameter of 5mm and an outer diameter of 7 mm.
8. The laboratory simple methane gas producing and collecting apparatus as set forth in claim 1, wherein the gas collecting structure is provided with an adapter, a vacuum aluminum foil air bag, and an injector, the adapter being connected to the second elastic rubber tube, the adapter being mounted on the vacuum aluminum foil air bag.
9. The laboratory simple methane gas production and collection device of claim 8, wherein the syringe is a 50mL syringe, the vacuum aluminum foil bladder is 12 x 14cm in size, and the adapter is a single valve switch.
10. A laboratory simple gas preparation and collection method using the laboratory simple methane gas preparation and collection device according to any one of claims 1 to 9, the laboratory simple gas preparation and collection method comprising:
the liquid experimental sample is placed at the bottom of the conical flask, and the volume is 50 mL; after the bottle mouth is blocked by the silica gel plug, sealing and reinforcing the joint of the bottle mouth by using a sealing film in a laboratory;
the first glass conduit and the second glass conduit in the gas transition structure are dipped with a small amount of ultrapure water and then are plugged into the double holes of the silica gel plug, and then elastic rubber pipes with proper lengths are respectively connected;
a first elastic rubber tube connected with a first glass guide tube in the gas collecting structure is clamped by a water stop clamp in the gas preparation process, and the water stop clamp is opened in the gas collecting process;
the second glass conduit is connected with the adapter through a second elastic rubber tube and then connected with the injector, saturated saline is injected, and gas in 200mL of samples is collected by a drainage and gas collection method; after the gas is collected, the adapter is closed, and the opening of the adapter is tightly wrapped and sealed by a sealing film of a laboratory.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202210493639.2A CN114789027A (en) | 2022-05-07 | 2022-05-07 | Simple methane gas preparation and collection device and method for laboratory |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202210493639.2A CN114789027A (en) | 2022-05-07 | 2022-05-07 | Simple methane gas preparation and collection device and method for laboratory |
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| CN215048857U (en) * | 2021-03-23 | 2021-12-07 | 北京师范大学 | Photocatalysis water treatment experimental device |
| CN114814086A (en) * | 2022-03-30 | 2022-07-29 | 长沙标朗住工科技有限公司 | White smoke collecting device for experiment and application thereof |
| CN217277191U (en) * | 2022-01-25 | 2022-08-23 | 聊城地震监测中心站 | Novel gaseous sample of groundwater solution gas device |
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2022
- 2022-05-07 CN CN202210493639.2A patent/CN114789027A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203552565U (en) * | 2013-10-16 | 2014-04-16 | 杨奕杰 | Experimental device for active metal reacting with water |
| CN105903504A (en) * | 2016-06-21 | 2016-08-31 | 夏攸阳 | Storage device for classroom lab gas |
| CN215048857U (en) * | 2021-03-23 | 2021-12-07 | 北京师范大学 | Photocatalysis water treatment experimental device |
| CN217277191U (en) * | 2022-01-25 | 2022-08-23 | 聊城地震监测中心站 | Novel gaseous sample of groundwater solution gas device |
| CN114814086A (en) * | 2022-03-30 | 2022-07-29 | 长沙标朗住工科技有限公司 | White smoke collecting device for experiment and application thereof |
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