CN114751354A - Pathogenic microorganism transfer liquid preparation device and preparation method of transfer liquid - Google Patents
Pathogenic microorganism transfer liquid preparation device and preparation method of transfer liquid Download PDFInfo
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- CN114751354A CN114751354A CN202210416958.3A CN202210416958A CN114751354A CN 114751354 A CN114751354 A CN 114751354A CN 202210416958 A CN202210416958 A CN 202210416958A CN 114751354 A CN114751354 A CN 114751354A
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- 239000007788 liquid Substances 0.000 title claims abstract description 110
- 238000012546 transfer Methods 0.000 title claims abstract description 56
- 244000000010 microbial pathogen Species 0.000 title claims abstract description 36
- 238000002360 preparation method Methods 0.000 title claims abstract description 27
- 238000011049 filling Methods 0.000 claims abstract description 69
- 238000003756 stirring Methods 0.000 claims abstract description 55
- 238000003860 storage Methods 0.000 claims abstract description 30
- 238000002156 mixing Methods 0.000 claims abstract description 14
- 239000007921 spray Substances 0.000 claims description 24
- 230000005540 biological transmission Effects 0.000 claims description 23
- 230000002779 inactivation Effects 0.000 claims description 21
- 150000002357 guanidines Chemical class 0.000 claims description 19
- 230000000415 inactivating effect Effects 0.000 claims description 12
- 239000003795 chemical substances by application Substances 0.000 claims description 11
- 230000000937 inactivator Effects 0.000 claims description 10
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 9
- 239000012530 fluid Substances 0.000 claims description 9
- 238000005192 partition Methods 0.000 claims description 8
- 239000003755 preservative agent Substances 0.000 claims description 6
- 230000002335 preservative effect Effects 0.000 claims description 6
- 239000004094 surface-active agent Substances 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 5
- 239000010902 straw Substances 0.000 claims description 5
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims description 3
- 239000003109 Disodium ethylene diamine tetraacetate Substances 0.000 claims description 3
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 claims description 3
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims description 3
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims description 3
- 235000019301 disodium ethylene diamine tetraacetate Nutrition 0.000 claims description 3
- YQOKLYTXVFAUCW-UHFFFAOYSA-N guanidine;isothiocyanic acid Chemical compound N=C=S.NC(N)=N YQOKLYTXVFAUCW-UHFFFAOYSA-N 0.000 claims description 3
- 239000003112 inhibitor Substances 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 238000012544 monitoring process Methods 0.000 claims description 3
- 239000002736 nonionic surfactant Substances 0.000 claims description 3
- 239000003161 ribonuclease inhibitor Substances 0.000 claims description 3
- 239000008363 phosphate buffer Substances 0.000 claims description 2
- 238000012545 processing Methods 0.000 claims description 2
- 244000052769 pathogen Species 0.000 abstract description 30
- 238000001514 detection method Methods 0.000 abstract description 15
- 241000700605 Viruses Species 0.000 abstract description 11
- 230000000694 effects Effects 0.000 abstract description 7
- 238000005070 sampling Methods 0.000 abstract description 4
- 241001493065 dsRNA viruses Species 0.000 abstract description 3
- 238000000926 separation method Methods 0.000 abstract description 3
- 241000894006 Bacteria Species 0.000 abstract description 2
- 239000003814 drug Substances 0.000 abstract 2
- 239000000243 solution Substances 0.000 description 40
- 230000001717 pathogenic effect Effects 0.000 description 21
- 108020004707 nucleic acids Proteins 0.000 description 6
- 150000007523 nucleic acids Chemical class 0.000 description 6
- 102000039446 nucleic acids Human genes 0.000 description 6
- 239000011324 bead Substances 0.000 description 4
- 238000005422 blasting Methods 0.000 description 4
- 230000009849 deactivation Effects 0.000 description 3
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 2
- 208000036142 Viral infection Diseases 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 238000009833 condensation Methods 0.000 description 2
- 230000005494 condensation Effects 0.000 description 2
- ZRALSGWEFCBTJO-UHFFFAOYSA-O guanidinium Chemical compound NC(N)=[NH2+] ZRALSGWEFCBTJO-UHFFFAOYSA-O 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 230000009385 viral infection Effects 0.000 description 2
- 102000016911 Deoxyribonucleases Human genes 0.000 description 1
- 108010053770 Deoxyribonucleases Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- ZJYYHGLJYGJLLN-UHFFFAOYSA-N guanidinium thiocyanate Chemical compound SC#N.NC(N)=N ZJYYHGLJYGJLLN-UHFFFAOYSA-N 0.000 description 1
- 239000008055 phosphate buffer solution Substances 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 108091092562 ribozyme Proteins 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Images
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B67—OPENING, CLOSING OR CLEANING BOTTLES, JARS OR SIMILAR CONTAINERS; LIQUID HANDLING
- B67C—CLEANING, FILLING WITH LIQUIDS OR SEMILIQUIDS, OR EMPTYING, OF BOTTLES, JARS, CANS, CASKS, BARRELS, OR SIMILAR CONTAINERS, NOT OTHERWISE PROVIDED FOR; FUNNELS
- B67C3/00—Bottling liquids or semiliquids; Filling jars or cans with liquids or semiliquids using bottling or like apparatus; Filling casks or barrels with liquids or semiliquids
- B67C3/02—Bottling liquids or semiliquids; Filling jars or cans with liquids or semiliquids using bottling or like apparatus
- B67C3/22—Details
- B67C3/24—Devices for supporting or handling bottles
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B67—OPENING, CLOSING OR CLEANING BOTTLES, JARS OR SIMILAR CONTAINERS; LIQUID HANDLING
- B67C—CLEANING, FILLING WITH LIQUIDS OR SEMILIQUIDS, OR EMPTYING, OF BOTTLES, JARS, CANS, CASKS, BARRELS, OR SIMILAR CONTAINERS, NOT OTHERWISE PROVIDED FOR; FUNNELS
- B67C3/00—Bottling liquids or semiliquids; Filling jars or cans with liquids or semiliquids using bottling or like apparatus; Filling casks or barrels with liquids or semiliquids
- B67C3/02—Bottling liquids or semiliquids; Filling jars or cans with liquids or semiliquids using bottling or like apparatus
- B67C3/16—Bottling liquids or semiliquids; Filling jars or cans with liquids or semiliquids using bottling or like apparatus using suction
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B67—OPENING, CLOSING OR CLEANING BOTTLES, JARS OR SIMILAR CONTAINERS; LIQUID HANDLING
- B67C—CLEANING, FILLING WITH LIQUIDS OR SEMILIQUIDS, OR EMPTYING, OF BOTTLES, JARS, CANS, CASKS, BARRELS, OR SIMILAR CONTAINERS, NOT OTHERWISE PROVIDED FOR; FUNNELS
- B67C3/00—Bottling liquids or semiliquids; Filling jars or cans with liquids or semiliquids using bottling or like apparatus; Filling casks or barrels with liquids or semiliquids
- B67C3/02—Bottling liquids or semiliquids; Filling jars or cans with liquids or semiliquids using bottling or like apparatus
- B67C3/22—Details
- B67C3/26—Filling-heads; Means for engaging filling-heads with bottle necks
- B67C3/2634—Filling-heads; Means for engaging filling-heads with bottle necks specially adapted for vacuum or suction filling
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B67—OPENING, CLOSING OR CLEANING BOTTLES, JARS OR SIMILAR CONTAINERS; LIQUID HANDLING
- B67C—CLEANING, FILLING WITH LIQUIDS OR SEMILIQUIDS, OR EMPTYING, OF BOTTLES, JARS, CANS, CASKS, BARRELS, OR SIMILAR CONTAINERS, NOT OTHERWISE PROVIDED FOR; FUNNELS
- B67C3/00—Bottling liquids or semiliquids; Filling jars or cans with liquids or semiliquids using bottling or like apparatus; Filling casks or barrels with liquids or semiliquids
- B67C3/02—Bottling liquids or semiliquids; Filling jars or cans with liquids or semiliquids using bottling or like apparatus
- B67C3/22—Details
- B67C3/28—Flow-control devices, e.g. using valves
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a preparation device and a preparation method of a pathogenic microorganism transfer liquid, and the preparation device comprises an equipment compartment, a liquid mixing mechanism, a filling mechanism and a control terminal, wherein the liquid mixing mechanism is positioned at one end in the equipment compartment, the liquid mixing mechanism comprises a first storage tank, a second storage tank, a third conveying pump and a stirring tank, and the filling mechanism is positioned at the other end in the equipment compartment and comprises a lower conveying belt, an upper conveying belt and a lifting mechanism; the preparation method of the transfer liquid comprises medicament mixing and medicament filling. The preparation device of the pathogenic microorganism transfer liquid realizes the automatic filling of the transfer liquid, is light and convenient to move, meets the sudden and large-scale detection requirements of pathogenic microorganisms in local areas, is suitable for the transfer of bacteria samples and RNA virus samples or DNA virus samples after sampling, can furthest maintain the activity of pathogens by using the non-inactivated transfer liquid, and can be subsequently used for the detection of the pathogens as well as the culture and separation of the pathogens.
Description
Technical Field
The invention relates to the technical field of pathogenic microorganism transfer liquid, in particular to a preparation device and a preparation method of pathogenic microorganism transfer liquid.
Background
In order to fully analyze the cause of viral infection, it is often necessary to collect and extract the virus from the patient and to detect the virus by analysis to determine the type of viral infection. The living virus can maintain the originality of the sample to the maximum extent, and can be used for culturing and separating the virus and various molecular biological tests of the virus. The pathogenic microorganism transfer liquid on the market at present cannot be simultaneously suitable for transfer after sampling of a bacterial sample and a virus sample (the virus sample comprises an RNA virus sample and a DNA virus sample), and the transfer liquid is difficult to store the activity of pathogens or has poor survival capability of the pathogens stored in the transfer liquid, so that the subsequent detection of pathogenic microorganisms is inconvenient; and the existing transfer liquid is manufactured by merchants in factories, and the convenience of use is influenced by the transportation timeliness, so that the detection requirements of sudden and large-scale pathogenic microorganisms are particularly difficult to meet.
Therefore, a preparation device and a preparation method of the pathogenic microorganism transport solution are designed to solve the problems.
Disclosure of Invention
The invention aims to solve the defects that a pathogenic microorganism transfer liquid in the prior art cannot be simultaneously suitable for transferring a bacteria sample and a virus sample after sampling, the activity of a pathogen is difficult to store in the transfer liquid, and the subsequent detection of pathogenic microorganisms is inconvenient.
In order to achieve the purpose, the invention adopts the following technical scheme:
a pathogenic microorganism transfer liquid preparation device comprises an equipment compartment, a liquid mixing mechanism, a filling mechanism and a control terminal, wherein the liquid mixing mechanism is positioned at one end in the equipment compartment and comprises a first storage box, a second storage box, a third delivery pump and a stirring tank, the filling mechanism is positioned at the other end in the equipment compartment and comprises a lower conveying belt, an upper conveying belt and a lifting mechanism, a first solution tank is stored in the first storage box and is connected with the input end of the first delivery pump through a first suction pipe, the output end of the first delivery pump is connected with the first conveying pipe, a second solution tank is stored in the second storage box and is connected with the input end of the second delivery pump through a second suction pipe, the output end of the second delivery pump is connected with the second conveying pipe, the first conveying pipe and the second conveying pipe are respectively communicated with a first third branch pipe, the first three branch pipes are divided into three guide pipes and are respectively connected with the stirring tank, the lower end part of the stirring tank is respectively led out of the output end to be connected with a second three branch pipe, the second three branch pipe is connected with a third delivery pump through a third suction pipe, the output end of the third delivery pump is connected with a third delivery pipe, the third delivery pipe is connected with a spray pipe, and a plurality of nozzles are uniformly distributed on the spray pipe.
Furthermore, the equipment compartment is of a compartment-type movable structure, a bottom cushion plate is arranged at the bottom of the equipment compartment, four corners of the lower part of the bottom cushion plate are respectively provided with universal wheels, the first conveying pump, the second conveying pump, the first flow meter, the second flow meter and the third conveying pump are respectively and fixedly arranged on a bearing plate, the bearing plate is of a strip-shaped plate-shaped structure, two ends of the bearing plate are respectively fixed on two sides of the inner wall of the compartment body of the equipment compartment, the top of one side of the spray pipe is provided with a placing platform, the other side of the spray pipe is provided with a collecting port, two side surfaces of the equipment compartment, which correspond to the first storage box and the second storage box, are respectively provided with a liquid storage port, one side of the equipment compartment, which corresponds to the lower conveying belt, is provided with a conveying port, the lower port of which is lower than the upper surface of the lower conveying belt, the upper end surface of which is not higher than the lower surface of the upper conveying belt, the upper surface of the upper conveying belt is positioned in the collecting port, and two sides of the collecting port are respectively provided with a limit baffle plate for limiting the movement of the filling support, and the lower bottom end of the limit baffle plate is supported on the bottom cushion plate.
Further, a straight-through pipe is arranged on a pipeline, positioned between the first flowmeter and the first three branch pipes, on the first conveying pipe, the straight-through pipe is communicated with the spray pipe and the first conveying pipe, a first electromagnetic valve is arranged on the straight-through pipe, a second electromagnetic valve is arranged between the straight-through pipe and the first three branch pipes, a screwing switch is arranged on the nozzle, the nozzle corresponds to the solution bottle on the filling support, an upper conveying belt and a lower conveying belt are respectively arranged on the lower side of the nozzle at one time, the lower conveying belt and the upper conveying belt are used for conveying the filling support, a lifting mechanism is arranged on the same side of the upper conveying belt and the lower conveying belt, the lifting mechanism comprises a frame and a motor, the frame is of an Jiong-shaped structure, a partition plate is embedded in the frame, the spray pipe is arranged at the top end of the frame, motors are respectively arranged on two sides of the upper part of the frame, and a belt is arranged at the output end of the motor, and a plurality of lifting plates are uniformly and fixedly arranged on the belt.
Furthermore, the filling support is of a cuboid structure, the upper half part of the filling support is uniformly provided with containing cavities along the axial direction, solution bottles are inserted into the containing cavities, the number of the containing cavities is not less than that of the nozzles, the length of the filling support is less than the width of the lower conveying belt, the length of the filling support is the same as that between the outer sides of the lifting plates at two sides, the lower conveying belt is internally provided with not less than three lower transmission rods, the upper conveying belt is internally provided with not less than three upper transmission rods, two ends of the lower transmission rods and the upper transmission rods are fixed on the limit baffle, one sides of the lower conveying belt and the upper conveying belt, which are close to the lifting mechanism, are respectively provided with a lower guide slide block and an upper guide slide block, the lower guide slide block and the upper guide slide block are both positioned between the lifting plates, and the included angle between the lifting plates and the belt ranges from eighty degrees to eighty-fifty degrees, the cross section of leading the slider down and leading the slider on is the right angled triangle structure, the right angle side of leading the slider down is close to conveyer belt one side down, the right angle side of leading the slider on is close to one side of baffle, the top of baffle is provided with fretwork portion, the lower surface of fretwork portion is provided with the push pedal, the push pedal is platelike structure, its one side with the filling holds in the palm correspondingly, and the middle part of opposite side is connected with the output conduction pole of servo electric jar, servo electric jar is fixed in through bearing plate on the baffle.
Further, the agitator tank sets up threely, arranges in proper order respectively, and is three the honeycomb duct stretches into in the agitator tank, just the bottom of honeycomb duct is located between the centre to the top of agitator tank, the output that the lower tip of agitator tank was drawn forth with be provided with the third solenoid valve between the third branch pipe of second respectively, the agitator tank is including a jar body, base and stirring rod, the base is fixed in on the backing plate, the jar body is cylindric cavity structure, and is fixed in the base upper surface, its top adopt uncovered or closed form, and inside bottom surface is provided with the stirring rod.
Furthermore, the control terminal is a programmable logic controller and comprises a central processing unit, a storage unit, an input unit and an output unit, and the control terminal is electrically connected with the first delivery pump, the first flowmeter, the first electromagnetic valve, the second delivery pump, the second flowmeter, the third delivery pump, the stirring tank, the third electromagnetic valve, the lower transmission rod, the upper transmission rod and the servo electric cylinder respectively.
A preparation method of a transport solution of a pathogenic microorganism transport solution preparation device comprises the following steps:
firstly, injecting a non-inactivated transfer liquid into a first solution tank, injecting a guanidine salt inactivating agent into a second solution tank, storing, sequentially placing filling supports on a lower conveying belt through conveying ports on an equipment compartment, starting a first conveying pump and a second conveying pump through a control terminal to inject the non-inactivated transfer liquid in the first solution tank and the guanidine salt inactivating agent in the second solution tank into a stirring tank, and monitoring the conveying amount of liquid in the first conveying pipe and the second conveying pipe in real time through a first flowmeter and a second flowmeter during liquid conveying, wherein the first electromagnetic valve is in a closed state and the second electromagnetic valve is in an open state;
step two, after the liquid in the first conveying pipe and the second conveying pipe reaches the stirring tank, starting the stirring tank to drive the stirring rod inside to rotate, stirring the injected mixed liquid of the non-inactivation transfer liquid and the guanidine salt inactivator to fully mix the liquid, wherein the mixed liquid is the inactivation transfer liquid;
step three, after the non-inactivated transfer liquid and the guanidine salt inactivating agent are mixed to obtain inactivated transfer liquid in the step two, starting a lower conveying belt, an upper conveying belt and a lifting mechanism, conveying the filling support from the lower conveying belt to the top of the lifting mechanism through the lifting mechanism, starting a third conveying pump, gradually starting a third electromagnetic valve, enabling the inactivated transfer liquid in the stirring tank to sequentially pass through a third suction pipe, a third conveying pipe and a spray pipe, and injecting the inactivated transfer liquid into a solution bottle in the filling support through nozzles uniformly distributed on the spray pipe to finish filling work;
and step four, finally, pushing a push plate through an output end of the servo electric cylinder to push out the filling support after filling is finished, sliding the filling support onto an upper conveying belt along an upper guide slide block, and conveying the filling support to a collecting port on one side of the equipment compartment through the upper conveying belt for collecting.
Further, the non-inactivating transport solution comprises a phosphate buffer, an rnase inhibitor, a dnase inhibitor, disodium EDTA, a biological preservative, a surfactant, and trehalose or glycerol, and the guanidine salt inactivator comprises guanidine isothiocyanate.
Further, the surfactant is a nonionic surfactant, and the biological preservative is Proclin 300.
Furthermore, in the first step, the second delivery pump and the second electromagnetic valve are closed, the first delivery pump and the first electromagnetic valve are opened, and the non-inactivated transport liquid in the first solution tank is injected into the solution bottle in the filling support through the nozzle.
Compared with the prior art, the invention has the beneficial effects that:
1. according to the pathogenic microorganism transport liquid preparation device, the first delivery pump, the second delivery pump, the third delivery pump and the connecting pipeline are respectively arranged, so that the non-inactivated transport liquid in the first solution tank can be directly filled into the solution bottle of the filling support through the first delivery pump and the third delivery pump; when liquid is transported in needs use inactivation, through the cooperation of first delivery pump, second delivery pump and third delivery pump, transport liquid and guanidine salt inactivator stirring misce bene in the agitator tank with non-inactivation, it uses to form the inactivation transport liquid, through setting up down the conveyer belt, go up the automatic filling that conveyer belt and lifting mechanism realized transporting the liquid, the preparation facilities is light and handy to be removed conveniently, do not receive the space region restriction, satisfy local area proruption, large-scale pathogenic microorganism's detection demand.
2. The pathogenic microorganism transfer liquid is simultaneously suitable for transferring after sampling a bacterial sample and an RNA virus sample or a DNA virus sample; the inactivated transfer solution can maintain the activity of the pathogen to the maximum extent, and can be subsequently used for not only the detection of the pathogen, but also the culture and separation of the pathogen.
3. The inactivated transport fluid for pathogenic microorganisms provided by the invention can inhibit the activities of RNA enzyme and DNA enzyme, preserve the integrity of RNA and DNA, and realize normal-temperature preservation and transportation of samples, wherein the inactivated transport fluid can rapidly inactivate pathogens at room temperature, reduce the infection risk of operators, reduce the false negative of detection, and can directly crack the pathogens to release nucleic acid, thereby being convenient for subsequent nucleic acid detection.
Drawings
FIG. 1 is a schematic view showing the overall structure of an equipment compartment according to the present invention;
FIG. 2 is a schematic view of the interior of the equipment compartment of the present invention from a first perspective;
FIG. 3 is a schematic view of a second perspective of the interior structure of the equipment compartment of the present invention;
FIG. 4 is a schematic structural view of a liquid mixing mechanism according to the present invention;
FIG. 5 is a schematic diagram of the filling mechanism of the present invention;
FIG. 6 is a schematic view of a piping connection structure according to the present invention;
FIG. 7 is an enlarged view of the nozzle structure of the present invention;
FIG. 8 is an enlarged view of the structure of the servo electric cylinder according to the present invention;
FIG. 9 is a schematic view of the construction of the stirring tank of the present invention;
FIG. 10a is a schematic view of an exploded assembly of the bead-type collection set according to the fourth embodiment of the present invention;
fig. 10b is a schematic view of a pathogen collecting tube and a bead blasting structure disclosed in the fourth embodiment of the present invention.
The reference numbers in the figures: 1. a transfer pipe cold plate; 2. a pathogen collecting tube; 3. bead blasting; 4. accommodating a tank; 10. equipment compartments, 11, a placing table; 12. a liquid storage port; 13. a delivery port; 14. a collection port; 15. a bottom bolster plate; 16. a support plate; 17. filling and supporting; 18. a partition plate; 19. a limiting baffle plate; 20. a first storage tank; 21. a first delivery pump; 22. a first straw; 23. a first delivery pipe; 24. a first flow meter; 25. a first solution tank; 26. a straight-through pipe; 27. a first solenoid valve; 28. a second solenoid valve; 30. a second storage tank; 31. a second delivery pump; 32. a second straw; 33. a second delivery pipe; 34. a second flow meter; 35. a second solution tank; 40. a third delivery pump; 41. a third straw; 42. a third delivery pipe; 43. a nozzle; 44. a nozzle; 45. screwing a switch; 46. a first third branch pipe; 47. a flow guide pipe; 48. a second third branch pipe; 50. a stirring tank; 51. a third electromagnetic valve; 52. a tank body; 53. a base; 54. a stirring rod; 60. a lower conveyor belt; 61. a lower transmission rod; 62. a lower guide slide block; 70. an upper conveying belt; 71. a transmission rod is arranged; 72. an upper guide slide block; 80. a lifting mechanism; 81. a frame; 82. a motor; 83. a belt; 84. lifting the plate; 90. a servo electric cylinder; 91. an output conduction rod; 92. pushing a plate; 93. the bearing plate is loaded.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
The first embodiment is as follows:
referring to fig. 1 to 3, a preparation device for pathogenic microorganism transportation liquid includes an equipment compartment 10, a liquid mixing mechanism, a filling mechanism and a control terminal, wherein the equipment compartment 10 is a compartment-type movable structure, a bottom pad 15 is arranged at the bottom of the equipment compartment, universal wheels are arranged at four corners of the lower portion of the bottom pad 15, a placing platform 11 is arranged at the top of one side of a spray pipe 43, a collecting port 14 is arranged at the other side of the spray pipe, liquid storage ports 12 are arranged at two side surfaces of the equipment compartment 10 corresponding to a first storage box 20 and a second storage box 30, a conveying port 13 is arranged at one side corresponding to a lower conveying belt 60, the lower port of the conveying port 13 is lower than the upper surface of the lower conveying belt 60, the upper end surface of the conveying belt is not higher than the lower surface of an upper conveying belt 70, the upper surface of the upper conveying belt 70 is located in the collecting port 14, two sides of the collecting port 14 are respectively provided with a limiting baffle 19 for limiting the movement of a filling support 17, the lower bottom end of the limit baffle 19 is supported on the bottom cushion plate 15, the first delivery pump 21, the second delivery pump 31, the first flowmeter 24, the second flowmeter 34 and the third delivery pump 40 are respectively and fixedly arranged on the support plate 16, the support plate 16 is a strip-shaped plate-shaped structure, and two ends of the support plate are respectively fixed on two sides of the inner wall of the equipment compartment 10.
As shown in fig. 3, 4 and 6, the liquid mixing mechanism is located at one end of the equipment compartment 10 and includes a first storage tank 20, a second storage tank 30, a third delivery pump 40 and a stirring tank 50, the filling mechanism is located at the other end of the equipment compartment 10 and includes a lower conveyor belt 60, an upper conveyor belt 70 and a lifting mechanism 80, the first storage tank 20 stores a first solution tank 25, the first solution tank 25 is connected to an input end of a first delivery pump 21 through a first suction pipe 22, an output end of the first delivery pump 21 is connected to a first delivery pipe 23, the second storage tank 30 stores a second solution tank 35, the second solution tank 35 is connected to an input end of a second delivery pump 31 through a second suction pipe 32, an output end of the second delivery pump 31 is connected to a second delivery pipe 33, the first storage tank 20 and the second storage tank 30 are temperature control tanks, a heating wire is arranged in the box body plate, a temperature sensor is arranged in the box, and the temperature sensor is connected with the control terminal; the first delivery pipe 23 and the second delivery pipe 33 are respectively communicated with a first three branch pipe 46, the first three branch pipe 46 is divided into three flow guide pipes 47 and is respectively connected with a stirring tank 50, the lower end part of the stirring tank 50 is respectively led out to be connected with an output end and a second three branch pipe 48, the second three branch pipe 48 is connected with a third delivery pump 40 through a third suction pipe 41, the output end of the third delivery pump 40 is connected with a third delivery pipe 42, the third delivery pipe 42 is connected with a spray pipe 43, a plurality of nozzles 44 are uniformly distributed on the spray pipe 43, a straight-through pipe 26 is arranged on the first delivery pipe 23 and positioned between the first flowmeter 24 and the first three branch pipe 46, the straight-through pipe 26 is communicated with the spray pipe 43 and the first delivery pipe 23, a first electromagnetic valve 27 is arranged on the straight-through pipe 26, and a second electromagnetic valve 28 is arranged between the straight-through pipe 26 and the first three branch pipe 46, the nozzle 44 is provided with a screw switch 45, and the nozzle 44 corresponds to the solution bottle on the filling support 17.
As shown in fig. 5, 7 and 8, an upper conveyor belt 70 and a lower conveyor belt 60 are respectively disposed on the lower side of the nozzle 44 at one time, the lower conveyor belt 60 and the upper conveyor belt 70 are used for conveying the filling support 17, a lifting mechanism 80 is disposed on the same side of the lower conveyor belt 60 and the upper conveyor belt 70, the lifting mechanism 80 includes a frame 81 and a motor 82, the frame 81 is in an Jiong-shaped structure, a partition plate 18 is embedded in the frame, the spray pipe 43 is disposed at the top end of the frame 81, the motors 82 are disposed on two sides of the upper portion of the frame 81, a belt 83 is disposed at the output end of the motor 82, and a plurality of lifting plates 84 are uniformly and fixedly disposed on the belt 83.
The filling support 17 is of a cuboid structure, the upper half part of the filling support 17 is uniformly provided with containing cavities along the axis direction, solution bottles are inserted into the containing cavities, the solution bottles are used for storing transfer liquid when filling, the number of the containing cavities is not less than the number of the nozzles 44, the length of the filling support 17 is smaller than the width of the lower conveying belt 60, the length of the filling support 17 is the same as the length between the outer sides of the lifting plates 84 on two sides, the lower conveying belt 60 is internally provided with at least three lower transmission rods 61, the upper conveying belt 70 is internally provided with at least three upper transmission rods 71, two ends of the lower transmission rods 61 and the upper transmission rods 71 are fixed on the limit baffle plate 19, one sides of the lower conveying belt 60 and the upper conveying belt 70, which are close to the lifting mechanism 80, are respectively provided with a lower guide slide block 62 and an upper guide slide block 72, and the lower guide slide block 62 and the upper guide slide block 72 are both positioned between the lifting plates 84, the included angle range of the lifting plate 84 and the belt 83 is eighty-five degrees, the cross sections of the lower guide slide block 62 and the upper guide slide block 72 are of right-angled triangle structures, the right-angled edge of the lower guide slide block 62 is close to one side of the lower conveying belt 60, the right-angled edge of the upper guide slide block 72 is close to one side of the partition plate 18, a hollow part is arranged at the top of the partition plate 18, a push plate 92 is arranged on the lower surface of the hollow part, the push plate 92 is of a plate-shaped structure, one side of the push plate 92 corresponds to the filling support 17, the middle part of the other side of the push plate 92 is connected with an output transmission rod 91 of a servo electric cylinder 90, and the servo electric cylinder 90 is fixed on the partition plate 18 through a bearing support plate 93.
As shown in fig. 6 and 9, three stirring tanks 50 are provided, which are respectively arranged in sequence, three flow guide pipes 47 extend into the stirring tank 50, the bottom of each flow guide pipe 47 is located between the middle and the top of the stirring tank 50, third electromagnetic valves 51 are respectively provided between the output end led out from the lower end of the stirring tank 50 and the second third branch pipe 48, the stirring tank 50 includes a tank body 52, a base 53 and a stirring rod 54, the base 53 is fixed on the bottom plate 15, the tank body 52 is a cylindrical cavity structure and is fixed on the upper surface of the base 53, the top end of the tank body adopts an open or closed form, the stirring rod 54 is provided on the inner bottom surface, the stirring tank 50 utilizes the characteristic that like poles of magnetic substances repel each other, the polarities at the two ends of the base 53 are continuously changed to push the stirring rod 54 to rotate, and the stirring rod 54 rotates to drive the liquid in the tank body 52 to rotate, the liquid is uniformly mixed, and when the stirring rod 54 rotates, the base 53 can perform a heating function to heat the mixed liquid in the tank 52, so that the liquid mixing speed is increased.
The preparation device of the pathogenic microorganism transfer liquid is respectively provided with a first delivery pump 21, a second delivery pump 31, a third delivery pump 40 and a connecting pipeline, so that the non-inactivated transfer liquid in the first solution tank 25 can be directly filled into the solution bottle of the filling support 17 through the first delivery pump 21 and the third delivery pump 40; when liquid is transported in the deactivation of needs use, through first delivery pump 21, second delivery pump 31 and third delivery pump 40's cooperation, transport liquid and guanidine salt inactivator stirring misce bene in agitator tank 50 with non-deactivation, it uses to form the deactivation transport liquid, through setting up down conveyer belt 60, go up the automatic filling that conveyer belt 70 and lifting mechanism 80 realized transporting the liquid, the preparation facilities is light and handy to be removed conveniently, do not receive the space region restriction, satisfy local area proruption, large-scale pathogenic microorganism's detection demand.
The second embodiment:
in this embodiment, the preparation method of the transport solution by using the apparatus for preparing a pathogenic microorganism transport solution in the first embodiment includes the following steps:
firstly, injecting non-inactivated transfer liquid into a first solution tank 25, injecting a guanidine salt inactivating agent into a second solution tank 35, storing, sequentially placing a filling support 17 on a lower conveying belt 60 through a conveying port 13 on an equipment compartment 10, starting a first conveying pump 21 and a second conveying pump 31 through a control terminal to inject the non-inactivated transfer liquid in the first solution tank 25 and the guanidine salt inactivating agent in the second solution tank 35 into a stirring tank 50, and monitoring the conveying amount of liquid in a first conveying pipe 23 and a second conveying pipe 33 in real time through a first flow meter 24 and a second flow meter 34 during liquid conveying, wherein the first electromagnetic valve 27 is in a closed state, and the second electromagnetic valve 28 is in an open state;
step two, after the liquid in the first delivery pipe 23 and the second delivery pipe 33 reaches the stirring tank 50, starting the stirring tank 50 to drive the stirring rod 54 inside to rotate, stirring the injected mixed liquid of the non-inactivation transfer liquid and the guanidine salt inactivator to fully mix the liquid, wherein the mixed liquid is the inactivation transfer liquid;
step three, after the non-inactivated transport liquid and the guanidine salt inactivating agent are mixed to obtain the inactivated transport liquid in the step two, starting a lower conveyer belt 60, an upper conveyer belt 70 and a lifting mechanism 80, conveying the filling support 17 from the lower conveyer belt 60 to the top of the lifting mechanism 80 through the lifting mechanism 80, then starting a third conveyer pump 40, then gradually starting a third electromagnetic valve 51, enabling the inactivated transport liquid in the stirring tank 50 to sequentially pass through a third suction pipe 41, a third conveyer pipe 42 and a spray pipe 43, and injecting the inactivated transport liquid into a solution bottle in the filling support 17 through nozzles 44 uniformly distributed on the spray pipe 43 to complete filling work;
and step four, finally, pushing a push plate 92 through the output end of the servo electric cylinder 90 to push out the filling support 17 after filling is completed, wherein the filling support 17 slides onto the upper conveying belt 70 along the upper guide slide block 72 and is conveyed to the collection port 14 at one side of the equipment compartment 10 through the upper conveying belt 70 for collection.
The pathogenic microorganism transfer liquid comprises an inactivated transfer liquid and a non-inactivated transfer liquid, wherein the non-inactivated transfer liquid comprises a phosphate buffer solution, an RNase inhibitor, a DNase inhibitor, disodium EDTA, a biological preservative, a surfactant and trehalose or glycerol, and the guanidine salt inactivator comprises guanidine isothiocyanate; the surfactant is a nonionic surfactant, the biological preservative is Proclin 300, the inactivated transfer liquid is obtained by fully mixing and reacting the inactivated transfer liquid and a guanidine salt inactivating agent, and the inactivated transfer liquid can directly crack pathogens to release nucleic acid, so that the method is convenient for subsequent nucleic acid detection.
Example three:
in this embodiment, when using pathogenic microorganism transportation liquid preparation facilities to carry out the transportation liquid preparation, through closing second delivery pump 31 and second solenoid valve 28, open first delivery pump 21 and first solenoid valve 27, inject the non-inactivation transportation liquid in the first solution jar 25 into the solution bottle in the filling support 17 through nozzle 44, directly carry out the filling use to the non-inactivation transportation liquid, the activity of pathogen can be kept to the at utmost to the non-inactivation transportation liquid, it not only can be used for the detection of pathogen subsequently, also can be used for the cultivation and the separation of pathogen, the non-inactivation transportation liquid of pathogenic microorganism can restrain rnase and dnase activity, save RNA and DNA integrality, realize that the sample is preserved the transportation at normal temperature, wherein the inactivation transportation liquid can inactivate the pathogen fast at room temperature, reduce operating personnel's infection risk, reduce the false negative of detection.
Example four:
the embodiment also discloses a suit is gathered to pathogenic microorganism formula of exploding pearl, including pathogen collecting pipe 2, transport pipe condensation plate 1, explode pearl 3 and gather the swab, pathogen collecting pipe 2 places in holding tank 4 of transport pipe condensation plate 1, hold pathogen non-inactivation transfer liquid and explode pearl 3 in the pathogen collecting pipe 2, there is the guanidinium inactivator in the explode pearl 3, the guanidinium inactivator contains guanidinium isothiocyanate, pathogen collecting pipe 2 forms for the plastics preparation of variability, be convenient for pinch the deformation, guarantee to break explode pearl 3 in the pathogen collecting pipe 2. When detecting and collecting, the collection swab is used for collecting pathogens and then is put into a pathogen collection tube 2 filled with pathogen non-inactivation transfer liquid, the bead blasting 3 can be broken through according to detection requirements, and the guanidine salt inactivating agent flows out of the bead blasting 3 and is mixed with the pathogen non-inactivation transfer liquid, so that the pathogen non-inactivation liquid is changed into an inactivation reagent, the pathogen can be directly cracked to release nucleic acid, the subsequent nucleic acid detection is convenient, and the biological safety is ensured.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (10)
1. The preparation device of the pathogenic microorganism transport fluid comprises an equipment compartment (10), a fluid mixing mechanism, a filling mechanism and a control terminal, and is characterized in that the fluid mixing mechanism is positioned at one end in the equipment compartment (10) and comprises a first storage tank (20), a second storage tank (30), a third delivery pump (40) and a stirring tank (50), the filling mechanism is positioned at the other end in the equipment compartment (10) and comprises a lower conveying belt (60), an upper conveying belt (70) and a lifting mechanism (80), a first solution tank (25) is stored in the first storage tank (20), the first solution tank (25) is connected with the input end of the first delivery pump (21) through a first suction pipe (22), the output end of the first delivery pump (21) is connected with a first conveying pipe (23), a second solution tank (35) is arranged in the second storage tank (30), second solution jar (35) are through the input of second straw (32) connection second delivery pump (31), second conveyer pipe (33) is connected to the output of second delivery pump (31), first conveyer pipe (23) and second conveyer pipe (33) communicate first three branch pipe (46) respectively, first three branch pipe (46) divide three honeycomb duct (47) and are connected with agitator tank (50) respectively, the lower tip of agitator tank (50) is drawn forth the output respectively and is connected with second three branch pipe (48), second three branch pipe (48) are connected with third delivery pump (40) through third straw (41), the output of third delivery pump (40) is connected with third conveyer pipe (42), third conveyer pipe (42) are connected with spray tube (43), evenly distributed has a plurality of nozzle (44) on spray tube (43).
2. The device for preparing the pathogenic microorganism transfer liquid according to claim 1, wherein the equipment compartment (10) is of a compartment-type movable structure, a bottom cushion plate (15) is arranged at the bottom of the equipment compartment, universal wheels are arranged at four corners of the lower portion of the bottom cushion plate (15), the first conveying pump (21), the second conveying pump (31), the first flowmeter (24), the second flowmeter (34) and the third conveying pump (40) are respectively and fixedly arranged on a bearing plate (16), the bearing plate (16) is of a strip-shaped plate-shaped structure, two ends of the bearing plate are respectively and fixedly arranged at two sides of the inner wall of the compartment body of the equipment compartment (10), a placing platform (11) is arranged at the top of one side of the spray pipe (43), a collecting port (14) is arranged at the other side of the spray pipe, and liquid storage ports (12) are arranged on two side faces of the equipment compartment (10) corresponding to the first storage tank (20) and the second storage tank (30), a conveying opening (13) is formed in one side corresponding to the lower conveying belt (60), the lower end opening of the conveying opening (13) is lower than the upper surface of the lower conveying belt (60), the upper end surface of the conveying opening is not higher than the lower surface of the upper conveying belt (70), the upper surface of the upper conveying belt (70) is located in the collecting opening (14), limiting baffles (19) used for limiting the movement of the filling support (17) are arranged on two sides of the collecting opening (14) respectively, and the lower bottom end of each limiting baffle (19) is supported on the bottom cushion plate (15).
3. The device for preparing the pathogenic microorganism transfer liquid according to claim 2, wherein a straight pipe (26) is arranged on the first delivery pipe (23) between the first flowmeter (24) and the first third branch pipe (46), the straight pipe (26) is communicated with the spray pipe (43) and the first delivery pipe (23), a first electromagnetic valve (27) is arranged on the straight pipe (26), a second electromagnetic valve (28) is arranged between the straight pipe (26) and the first third branch pipe (46), a screwing switch (45) is arranged on the nozzle (44), the nozzle (44) corresponds to the solution bottle on the filling support (17), an upper conveyor belt (70) and a lower conveyor belt (60) are respectively arranged on the lower side of the nozzle (44), and the lower conveyor belt (60) and the upper conveyor belt (70) are used for conveying the filling support (17), the lifting mechanism (80) is arranged on the same side of the frame and the frame, the lifting mechanism (80) comprises a frame (81) and a motor (82), the frame (81) is of an Jiong-shaped structure, a partition plate (18) is embedded in the frame, the spray pipe (43) is arranged at the top end of the frame (81), the motors (82) are arranged on two sides of the upper portion of the frame (81) respectively, a belt (83) is arranged at the output end of each motor (82), and a plurality of lifting plates (84) are uniformly and fixedly arranged on the belt (83).
4. The device for preparing the pathogenic microorganism transfer liquid as claimed in claim 3, wherein the filling support (17) is of a cuboid structure, the upper half part of the filling support is uniformly provided with accommodating cavities along the axial direction, solution bottles are inserted in the accommodating cavities, the number of the accommodating cavities is not less than that of the nozzles (44), the length of the filling support (17) is less than that of the lower conveying belt (60), the length of the filling support (17) is the same as that between the outer sides of the lifting plates (84) at the two sides, the lower conveying belt (60) is provided with not less than three lower transmission rods (61), the upper conveying belt (70) is provided with not less than three upper transmission rods (71), the two ends of the lower transmission rods (61) and the upper transmission rods (71) are fixed on the limiting baffle (19), and one sides of the lower conveying belt (60) and the upper conveying belt (70) close to the lifting mechanism (80) are respectively provided with not less than three upper transmission rods (71), and the two ends of the lower transmission rods (61) and the upper transmission rods (71) are fixed on the limiting baffle (19) A lower guide slide block (62) and an upper guide slide block (72), wherein the lower guide slide block (62) and the upper guide slide block (72) are both positioned between the lifting plates (84), the included angle range of the lifting plate (84) and the belt (83) is eighty-fifty degrees, the cross sections of the lower guide sliding block (62) and the upper guide sliding block (72) are both in a right-angled triangle structure, the right-angle edge of the lower guide sliding block (62) is close to one side of the lower conveying belt (60), the right-angle edge of the upper guide sliding block (72) is close to one side of the clapboard (18), a hollow part is arranged at the top of the clapboard (18), a push plate (92) is arranged on the lower surface of the hollow part, the push plate (92) is of a plate-shaped structure, one side of the filling support corresponds to the filling support (17), the middle part of the other side is connected with an output transmission rod (91) of a servo electric cylinder (90), the servo electric cylinder (90) is fixed on the partition plate (18) through a bearing supporting plate (93).
5. The apparatus of claim 2, wherein the apparatus for preparing a transport solution for pathogenic microorganisms, the three stirring tanks (50) are arranged in turn, the three guide pipes (47) extend into the stirring tanks (50), and the bottom of the draft tube (47) is positioned between the middle and the top of the stirring tank (50), third electromagnetic valves (51) are respectively arranged between output ends led out from the lower end part of the stirring tank (50) and the second third branch pipes (48), the stirring tank (50) comprises a tank body (52), a base (53) and a stirring rod (54), the base (53) is fixed on the base plate (15), the tank body (52) is of a cylindrical cavity structure and is fixed on the upper surface of the base (53), the top end of the stirring rod is in an open or closed form, and the inner bottom surface of the stirring rod is provided with a stirring rod (54).
6. The device for preparing the pathogenic microorganism transfer liquid according to claim 1, wherein the control terminal is a programmable logic controller and comprises a central processing unit, a storage unit, an input unit and an output unit, and the control terminal is electrically connected with the first delivery pump (21), the first flow meter (24), the first electromagnetic valve (27), the second electromagnetic valve (28), the second delivery pump (31), the second flow meter (34), the third delivery pump (40), the stirring tank (50), the third electromagnetic valve (51), the lower transmission rod (61), the upper transmission rod (71) and the servo electric cylinder (90) respectively.
7. A method for preparing a transport fluid using the apparatus for preparing a transport fluid for pathogenic microorganisms according to any one of claims 1 to 6, comprising the steps of:
firstly, injecting a non-inactivation transfer liquid into a first solution tank (25), injecting a guanidine salt inactivating agent into a second solution tank (35), storing, sequentially placing a filling support (17) on a lower conveying belt (60) through a conveying port (13) on an equipment compartment (10), starting a first conveying pump (21) and a second conveying pump (31) through a control terminal to inject the non-inactivation transfer liquid in the first solution tank (25) and the guanidine salt inactivating agent in the second solution tank (35) into a stirring tank (50), and monitoring the conveying amounts of the liquid in a first conveying pipe (23) and a second conveying pipe (33) in real time through a first flowmeter (24) and a second flowmeter (34) during liquid conveying, wherein the first electromagnetic valve (27) is in a closed state, and the second electromagnetic valve (28) is in an open state;
step two, after the liquid in the first conveying pipe (23) and the second conveying pipe (33) reaches the stirring tank (50), starting the stirring tank (50) to drive the stirring rod (54) inside to rotate, stirring the injected mixed liquid of the non-inactivation transfer liquid and the guanidine salt inactivator to fully mix the liquid, wherein the mixed liquid is the inactivation transfer liquid;
step three, after the inactivated transport solution is obtained by mixing the non-inactivated transport solution and the guanidine salt inactivating agent in the step two, starting a lower conveyer belt (60), an upper conveyer belt (70) and a lifting mechanism (80), conveying the filling support (17) from the lower conveyer belt (60) to the top of the lifting mechanism (80) through the lifting mechanism (80), then starting a third conveyer pump (40), then sequentially starting a third electromagnetic valve (51), enabling the inactivated transport solution in the stirring tank (50) to sequentially pass through a third suction pipe (41), a third conveyer pipe (42) and a spray pipe (43), and injecting the inactivated transport solution into a solution bottle in the filling support (17) through nozzles (44) uniformly distributed on the spray pipe (43) to complete filling work;
and fourthly, finally, pushing a push plate (92) through an output end of the servo electric cylinder (90), pushing out the filling support (17) after filling is finished, sliding the filling support (17) onto an upper conveying belt (70) along an upper guide slide block (72), and conveying the filling support to a collection port (14) at one side of the equipment compartment (10) through the upper conveying belt (70) for collection.
8. The method of claim 7, wherein the inactivated transport solution includes phosphate buffer, rnase inhibitor, dnase inhibitor, disodium EDTA, a biological preservative, a surfactant, and trehalose or glycerol, and the guanidine salt inactivator includes guanidine isothiocyanate.
9. The method for preparing a transport fluid for a pathogenic microorganism transport fluid preparation device according to claim 8, wherein the surfactant is a nonionic surfactant, and the biological preservative is Proclin 300.
10. The method for preparing the transport solution of the device for preparing the transport solution of pathogenic microorganisms according to claim 7, wherein in the first step, the non-inactivated transport solution in the first solution tank (25) is injected into the solution bottle in the filling tray (17) through the nozzle (44) by closing the second delivery pump (31) and the second solenoid valve (28) and opening the first delivery pump (21) and the first solenoid valve (27).
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| CN202210416958.3A CN114751354A (en) | 2022-04-20 | 2022-04-20 | Pathogenic microorganism transfer liquid preparation device and preparation method of transfer liquid |
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