CN114747683B - Feed for improving birth weight of piglets and reducing stillbirth rate - Google Patents
Feed for improving birth weight of piglets and reducing stillbirth rate Download PDFInfo
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- CN114747683B CN114747683B CN202210439794.6A CN202210439794A CN114747683B CN 114747683 B CN114747683 B CN 114747683B CN 202210439794 A CN202210439794 A CN 202210439794A CN 114747683 B CN114747683 B CN 114747683B
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- vitamin
- feed
- adenosine
- sow
- piglets
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Abstract
The invention discloses a feed for improving the birth weight of piglets and reducing the stillbirth rate, which belongs to the technical field of livestock breeding and comprises the following components: corn, rice bran meal, broken rice, soybean meal, triticale, adenosine or adenylic acid, stone powder, calcium bicarbonate, lysine, threonine, salt, choline chloride, a mold remover, a preservative and a premix; wherein the content of adenosine or adenosine monophosphate is 0.1%. The feed provided by the invention can obviously promote the angiogenesis of the placenta, obviously improve the birth weight of piglets, reduce the stillbirth rate and improve the reproductive performance of sows.
Description
Technical Field
The invention relates to the technical field of livestock and poultry breeding, in particular to a feed for improving the birth weight of piglets and reducing the stillbirth rate.
Background
The number of weaned piglets per year (PSY) of each sow is a common index for measuring the productivity of sows in a pig farm. According to an InterPIG global pig raising report jointly made by the pig raising associations of countries in the world in 2018, PSY of Denmark and Netherlands is more than 30, PSY of developed countries such as America, brazil, germany is more than 25, and the big data of the pig industry in the whole country show that the average level of PSY of the industries in 2019 in China is only 19.49, and the space from the world level still exists. With the rapid development of the pig industry in China, genetic breeding technology and feeding management optimization become more mature, the total litter size of sows is increased continuously, but the efficiency is still very low compared with that of developed countries.
In the actual breeding production process in the prior art, the energy intake of sows in the gestation period is controlled by adopting limited feeding, so that the condition that the fat deposition of sows is too much to be beneficial to the breeding efficiency is prevented. However, in the current breeding environment where PSY is pursued, the limitation of feeding sows in the gestation period usually results in the serious shortage of nutrients taken by sows in the inner tubes of the sows through the placenta, and then a large number of low-birth-weight piglets are generated, even the stillbirth rate is greatly increased, and the reproductive performance of the sows is finally influenced.
Meanwhile, as the total litter size of sows increases, the number of Low Birth Weight piglets (LBW) also increases, and researches show that the Low Birth Weight piglets are accompanied by higher stillbirth rate and pre-weaning mortality rate, and the Birth rate of the Low Birth Weight piglets can even reach 25% in the breeding process.
In order to increase the PSY index in pig raising production and improve the birth weight of piglets, thereby improving the pig raising efficiency, adding a proper nutrition regulator in the daily ration of pregnant sows is a more ideal choice. However, the effect of different types and levels of nutritional regulators on the reproductive performance of sows in the prior art is also different. Therefore, screening and determining the daily ration nutrition regulator with the appropriate level has important practical significance for improving the birth weight of piglets, reducing the stillbirth rate and improving the reproductive performance.
Disclosure of Invention
The invention aims to provide a feed for improving the birth weight and reducing the stillbirth rate of piglets, which aims to solve the technical problems of low birth weight and high stillbirth rate of piglets and provides the following scheme for realizing the aim:
the first technical scheme is as follows: a feed for improving the birth weight and reducing the stillbirth rate of piglets is a complete ration with the mass percentage of 99.9 percent and adenosine or adenylic acid with the mass percentage of 0.1 percent.
Further, the complete ration comprises the following components: corn, rice bran meal, broken rice, soybean meal, triticale, stone powder, calcium bicarbonate, lysine, threonine, salt, choline chloride, a mildew removing agent, a preservative and a premix.
Further, the proportion of the complete ration components is as follows according to mass percentage: 34.68% of corn, 10% of rice bran meal, 16.67% of broken rice, 14.5% of soybean meal, 20% of triticale, 0.1% of adenosine or adenylic acid, 1.1% of stone powder, 0.96% of calcium bicarbonate, 0.1% of lysine, 0.05% of threonine, 0.4% of salt, 0.13% of choline chloride, 0.08% of mildew remover, 0.05% of preservative and 1.18% of premix.
Further, the premix comprises the following components: vitamin A, vitamin D3, vitamin C, vitamin E, vitamin K, vitamin B1, vitamin B2, vitamin B6, vitamin B12, pantothenic acid, nicotinic acid, folic acid, biotin, copper, iron, zinc, manganese, iodine and cobalt.
Further, when the total ration is 1kg, the premix comprises the following components in parts by weight: vitamin A12000 IU and vitamin D 3 4800IU, vitamin C200mg, vitamin E205 mg, vitamin K3.6 mg, and vitamin B 1 3.6mg of vitamin B 2 12mg of vitamin B 6 7.2mg of vitamin B 12 0.048mg, pantothenic acid 30.0mg, nicotinic acid 48.0mg, folic acid 8.6mg, biotin 0.6mg, copper 10.0mg, iron 130mg, zinc 60mg, manganese 45mg, iodine 0.3mg, and cobalt 0.1mg. .
The second technical scheme is as follows: the feed is applied to improving the birth weight of piglets and reducing the stillbirth rate.
The third technical scheme is as follows: the matched feeding method of the feed is characterized by comprising the following steps:
(1) The feed intake of each sow is 2kg in 65-100 days of gestation;
(2) The daily feed intake of each sow from 100 days of gestation to 3 days before delivery is 2.5kg;
(3) The daily feed intake of each sow from 2 days before delivery to delivery was 2kg.
Further, the daily feed in the step (1) and the step (2) is fed at one time, and the daily feed in the step (3) is fed by 2 times.
Furthermore, the sow is a Du-Dao-Dazhao ternary hybridization first-birth sow.
The invention discloses the following technical effects:
the sow gestation feed feeding method for improving the birth weight of piglets and reducing the stillbirth rate provided by the invention is used for dynamically feeding sows in the gestation stage, meets the nutritional energy requirement from the late gestation stage to delivery of the sows, and can be used for feeding the sows in two days before delivery in a grading manner, so that the difficulty in delivery of the sows can be relieved, and the stillbirth rate can be effectively reduced.
Compared with a control group, the sow pregnancy feed for improving piglet birth weight and reducing the stillbirth rate provided by the invention can improve the content of adenosine, adenylic acid and metabolites thereof in placenta, obviously promote the angiogenesis of placenta, obviously improve piglet birth weight, reduce the stillbirth rate and improve the reproductive performance of sows.
The invention researches the influence of 0.1 percent of adenosine or adenylic acid added into pregnant ration on the reproductive performance of sows, and provides data support and theoretical guidance for actually producing the feed for improving the reproductive performance of the sows.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.
FIG. 1 shows the influence of addition of 0.1% adenosine or adenylic acid to the pregnant ration on the reproductive performance of sows, wherein A is the newborn weight of piglets; b is the dead tyre rate; c is placenta efficiency; the dead fetus rate is checked by chi-square, and the rest data are expressed by mean value plus standard error; * P <0.05, P < 0.01;
FIG. 2 is a graph showing the analysis of the effect of addition of 0.1% of adenosine or adenylic acid to the placenta tissues of sows in the pregnancy diet, and the influence of adenylic acid and its metabolites, wherein A is adenosine, B is adenine, C is xanthine, and D is hypoxanthine; all data are expressed as mean + standard error, n =6; * P <0.05, P <0.01, P < 0.001, P < 0.0001;
FIG. 3 is a graph showing the analysis of the effect of adenosine, adenylic acid and its metabolites in the placental tissues of sows by adding 0.1% of adenosine or adenylic acid to the pregnant ration, wherein A is adenylic acid, B is S-adenosine-homocysteine, C is inosine, and D is uric acid; all data are expressed as mean + standard error, n =6; * P <0.05, P <0.01, P < 0.001, P < 0.0001;
FIG. 4 is a graph of H & E staining analysis of control group, added 0.1% adenosine and added 0.1% adenylate sow placental vascular density;
FIG. 5 is a graph showing the effect of pregnancy diet adding 0.1% adenosine or adenylic acid on the placental angiogenesis of sows; all data are expressed as mean ± sem, n =6; * P <0.05, P < 0.01;
FIG. 6 is a graph showing the analysis of the effect of addition of adenosine or adenylic acid 0.1% to the pregnant diet on the placental angiogenesis of sows; wherein a is the mRNA relative expression amount of PIGF; b is the mRNA relative expression quantity of VEGF-A; c is the mRNA relative expression quantity of VEGF-B; d is the mRNA relative expression quantity of VEGF-C; e is the mRNA relative expression amount of VEGF-D; all data are expressed as mean ± sem, n =6; * P <0.05, P < 0.01;
FIG. 7 is Sub>A graph showing the change in the expression level of VEGF-A protein in the group of 0.1% adenosine and adenylate in placental angiogenesis in sows;
fig. 8 is a graph of the analysis of the growth factors in blood vessels of control group, 0.1% adenosine added and 0.1% adenylate added sows, all data expressed as mean ± standard error, n =6; * P <0.05, P < 0.01.
Detailed Description
Reference will now be made in detail to various exemplary embodiments of the invention, the detailed description should not be construed as limiting the invention but as a more detailed description of certain aspects, features and embodiments of the invention.
It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. Further, for numerical ranges in this disclosure, it is understood that each intervening value, between the upper and lower limit of that range, is also specifically disclosed. Every smaller range between any stated value or intervening value in a stated range and any other stated or intervening value in a stated range is encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference herein for the purpose of disclosing and describing the methods and/or materials associated with the documents. In case of conflict with any incorporated document, the present specification will control.
It will be apparent to those skilled in the art that various modifications and variations can be made in the specific embodiments of the present disclosure without departing from the scope or spirit of the disclosure. Other embodiments will be apparent to those skilled in the art from consideration of the specification. The description and examples are intended to be illustrative only.
As used herein, the terms "comprising," "including," "having," "containing," and the like are open-ended terms that mean including but not limited to.
Reagents, methods and apparatus used in the present invention are conventional in the art unless otherwise indicated.
Unless otherwise specified, reagents and materials used in the present invention are commercially available, with 0.1% adenosine and adenosine being purchased from Kyoto Keppen, bio Inc. The preservative is sodium benzoate, and the mildew removing agent is: sodium diacetate.
The feed components in the embodiment are 'premix', and when the feed is 1kg of complete ration, the premix comprises the following components in mass:
vitamin A12000 IU and vitamin D 3 4800IU, vitamin C200mg, vitamin E205 mg, vitamin K3.6 mg, and vitamin B 1 3.6mg of vitamin B 2 12mg of vitamin B 6 7.2mg of vitamin B 12 0.048mg, pantothenic acid 30.0mg, nicotinic acid 48.0mg, folic acid 8.6mg, biotin 0.6mg, copper 10.0mg, iron 130mg, zinc 60mg, manganese 45mg, iodine 0.3mg, and cobalt 0.1mg. .
The protein content of the soybean meal used in the embodiment of the application is more than or equal to 43 percent.
Example 1
Sow pregnancy feed formula
According to the daily ration formula of the pregnant sow in the following embodiment of the invention, common commodity daily ration is used as a control, 0.1% of adenosine or 0.1% of adenylic acid in percentage by mass is respectively added into the common commodity daily ration, the specific feed intake and the nutrient components are shown in tables 2 and 3, and the influence of the addition of 0.1% of adenosine or adenylic acid into the pregnant daily ration on the stillbirth rate of the sow, the birth weight of piglets, the placenta angiogenesis and the reproductive performance is deeply researched.
Table 1 shows the effect of pregnancy diets supplemented with 0.1% adenosine or adenylic acid on sow body weight, backfat, all data are presented as mean standard error.
TABLE 1 Effect of pregnancy ration addition of 0.1% adenosine or adenylic acid on sow body weight, backfat
TABLE 2 sow gestation period feed intake (kg/d)
TABLE 3 pregnant sow feed ingredients and nutritional levels
Example 2
The influence of 0.1% of adenosine or adenylic acid added into the daily ration during the gestation period on the reproductive performance of the sows and the growth performance of the piglets is verified, and in order to verify the effect of the invention, the reproductive performance of the sows in the group with 0.1% of adenosine or adenylic acid and the growth performance of the piglets are compared and analyzed.
1. Test animal
The test animal is a healthy Du long and large ternary miscellaneous first-birth sow, and the test place is Shanjiazhai breeding farm of Xinxing agriculture and animal husbandry GmbH company in Wannian county of Shanxi province.
2. Test processing and grouping
In 65 days of gestation, 135 sows are selected according to body weight and backfat and divided into 3 groups randomly, namely a blank control group, a 0.1% adenosine group and a 0.1% adenosine group. Each group of 45 sows, each sow 1 repeat, and the feed is fed in a single column. The feeding period of the test daily ration is 65 days of pregnancy-delivery (the composition of feed raw materials is shown in table 3, and the feeding mode is shown in table 2).
3. Data processing and analysis
135 sows were enrolled for data analysis. Statistical analysis was performed for one replicate per sow. After the test data are arranged by Excel software, single-factor variance analysis is carried out by an SPSS program, wherein the piglet growth performance adopts mixed model variance for data analysis. All data were examined for normality and were analyzed by Kruskal-wallis for test analysis if they did not conform to normal distribution. If the difference is significant, duncan's multiple comparison is performed, and the dead fetus rate is tested by chi-square method. Results are expressed as mean ± sem, 0.05 < P < 0.10 indicates statistically varying trends, P <0.05 indicates significant differences, and P <0.01 indicates very significant differences. Mapping was done by GraphPad Prism 8 software.
4. Test results
The effective sample size statistics of the sow at each stage are shown in the table 4. From 65 days to 109 days of pregnancy, 1, 0 and 2 sows are respectively eliminated in each group due to sow diseases, nonpregnant abortion and the like.
TABLE 4 statistical information table of effective sample amount of each stage of sow
As can be seen from Table 5, compared with the control group, the group added with 0.1% of adenosine in the pregnant diet significantly improves the birth weight of piglets and reduces the stillbirth rate (P < 0.05); the group added with 0.1% adenosine and 0.1% adenosine in the pregnant diet remarkably improves the vitality and the placenta efficiency of the piglets (P is less than 0.01). However, the intervallic mutation coefficient of the group added with 0.1% of adenosine in the pregnant diet is extremely remarkably increased (P < 0.01).
TABLE 5 influence of the addition of 0.1% adenosine or adenylic acid to the pregnancy ration on the reproductive performance of sows
Example 3
Pregnant sow feed nutrient content added with 0.1% adenosine or adenylic acid
1. Test animal
The test animals are healthy and grown-up ternary hybrid first-farrowing sows in example 2, and the test site and method (feed and feeding amount) are the same as those in example 2.
2. Sample collection and testing method
A representative original sample was selected to be not less than 1000g. Dividing into 250g by quartering method, air drying or oven drying at 60 deg.C, grinding with plant sample pulverizer, and sieving with 0.42mm test sieve. Sealing in a sample bag, and storing in shade for determination.
The content of Crude Protein (CP), crude Fiber (CF) and Neutral Detergent Fiber (NDF) in the feed samples was determined analytically by the method referred to feed analysis and feed quality testing techniques (3 rd edition).
3. Data processing and analysis
The experimental data were collated with Excel software and analyzed for one-way variance using SPSS program. All data were examined for normality and were examined for non-conformity to normal distribution using Kruskal-wallis. The subjects with significant differences were subjected to Duncan's multiple comparisons. Results are expressed as mean ± sd, with 0.05 < P < 0.10 indicating a statistically variable trend, P <0.05 indicating significant differences, and P <0.01 indicating very significant differences.
4. Test results
As can be seen from table 6, the addition of 0.1% adenosine or adenylic acid during gestation had no significant effect on feed Crude Protein (CP), crude Fiber (CF) and Neutral Detergent Fiber (NDF) levels (P < 0.05).
TABLE 6 daily ration nutrient content of pregnant sows
Example 4
Influence of 0.1% adenosine or adenylic acid added in gestation period on adenosine, adenylic acid and metabolite thereof in sow placenta tissue
1. Test animal
The test animals were healthy and large three-way-breed primiparous sows from example 2, and the test site and method (feed and feeding amount) were the same as example 2.
2. Sample collection and testing method
During the sow's labor, the newborn piglets' umbilical cords are tied with a short cotton string, each cotton string labeled with a numbering label to ensure that each piglet matches their placenta. After the placenta of the sow is discharged and weighed, collecting a part of placenta and putting the part of placenta into liquid nitrogen for quick freezing.
Shearing the collected placenta tissue sample on dry ice or ice, weighing 40mg, wherein the mass deviation is less than 10%; the tissue sample, the magnetic beads and 400 mu L of ultrapure water are homogenized for three times, wherein the homogenization frequency is 30 times/second and 2 min; sucking 200 μ L of homogenate into a sterile enzyme-free centrifuge tube, adding 800 μ L of MeOH methanol/CAN acetonitrile (v: v, 1:1) (precooling for 30 min-20 in advance), vortexing for 30s, and performing water bath ultrasound at 4 ℃ for 10min; incubating at-20 deg.C for 1h, centrifuging at 14500rpm and 4 deg.C for 15min, collecting supernatant 1000 μ L, and blowing with nitrogen gas; adding 200 mu L of methanol/H 2 O water (v: v, 1:1) redissolved. Vortex 30s, water bath ultrasound at 4 ℃ for 10min,14500rpm, and centrifugation at 4 ℃ for 15min. The supernatant was transferred through a 0.22 μm organic filter into a sample vial (with a lined tube) and stored at-80 ℃.
3. Data processing and analysis
The experimental data were collated using Excel software and analyzed for one-way variance using the SPSS program. All data were examined for normality and were analyzed by Kruskal-wallis for test analysis if they did not conform to normal distribution. Duncan's multiple comparisons were performed for those with significant differences. Results are expressed as mean ± sem, × P <0.05, × P <0.01, × P < 0.001, × P < 0.0001. Mapping was done with GraphPad Prism 8 software.
4. Test results
As shown in fig. 2 and 3, adenosine, adenylic acid and its metabolites were significantly increased in the placenta tissue of the 0.1% adenosine and adenylic acid group as compared to the control group.
Example 5
Effect of 0.1% adenosine or adenylic acid addition during gestation on placental angiogenesis of sows
1. Test animal
The test animals were healthy and large three-way-breed primiparous sows from example 2, and the test site and method (feed and feeding amount) were the same as example 2.
2. Sample collection and testing method
During the sow's parturition, the umbilical cords of the newborn piglets are tied with a short cotton thread, each of which is labeled with a number label, to ensure that each piglet matches their placenta. After the placenta is discharged from the sow, weighing the placenta, collecting a part of the placenta, and quickly freezing the part of the placenta in liquid nitrogen. The remaining fresh placenta tissue was immediately fixed with 4% paraformaldehyde.
(1) Placental vascular density
Fresh placenta tissue is fixed by 4% paraformaldehyde, embedded in paraffin to make 5 μm thick section, and then stained with hematoxylin-eosin. The area occupied by the placental tissue was followed and the placental blood vessels in these areas were followed using a projection microscope (olympus CX41, japan). Next, placental blood vessel area was quantified by Image J Image analysis and the relative number of placental blood vessels per unit tissue area was assessed.
(2) Expression level of placenta angiogenic factor mRNA
Total RNA was extracted from placental tissue using EZB reagent (Invitgen, carlsad, USA). cDNA synthesis was performed using PrimeScript RT kit (Dalian Kara, china). PCR reactions were performed using ABI QuantStudio 6Flex System (applied biosystems, calsbards, calif.) and SYBR Green kit (Genstar, china, beijing). The expression of the relevant gene was expressed as the ratio of the target gene to the control gene using 18S rRNA as the control gene.
(3) Expression level of placental angiogenic factor protein
The placenta was lysed with RIPA buffer (Beyotime, china, beijing) containing protease and phosphatase inhibitors. Protein concentrations were determined using BCA protein assay kit (Beyotime, china, beijing) according to manufacturer's instructions, then separated by SDS-PAGE and blotted onto PVDF membranes. Next, the blot was incubated overnight at 4 ℃ with the following primary antibody: VEGF-A (Proteintech, USA), betSub>A-Actin (Cst, USA). The band density was quantified using Image J software (national institute of health, usa, bessel, MD) and then normalized to β -Actin.
3. Data processing and analysis
After the test data are arranged by Excel software, single-factor variance analysis is carried out by an SPSS program, wherein the piglet growth performance adopts mixed model variance for data analysis. All data were examined for normality and were examined for non-conformity to normal distribution using Kruskal-wallis. Duncan's multiple comparisons were performed for those with significant differences. Results are expressed as mean ± sd, with 0.05 < P < 0.10 indicating a statistically variable trend, P <0.05 indicating significant differences, and P <0.01 indicating very significant differences. Mapping was done by GraphPad Prism 8 software.
4. Test results
(1) As shown in fig. 4 and 5, placental vascular density was significantly increased in the 0.1% adenosine group and the 0.1% adenylate group (P < 0.05) compared to the control group.
(2) As shown in FIG. 6, the mRNA relative expression levels of VEGF-A and VEGF-D were significantly increased in the 0.1% adenosine group compared to the control group; the mRNA relative expression of PIGF and VEGF-D in the 0.1% adenylate group is obviously improved.
(3) As shown in FIGS. 7 and 8, the VEGF-A protein expression level was significantly increased in the 0.1% adenosine and adenylate groups compared to the control group (P < 0.05).
The results of the above examples show that the addition of 0.1% adenosine or adenylic acid to the pregnant diet significantly increases the content of adenosine, adenylic acid and metabolites thereof in the placenta, significantly promotes the angiogenesis of the placenta, significantly increases the birth weight of piglets, reduces the stillbirth rate, and improves the reproductive performance of sows.
The above-described embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solutions of the present invention can be made by those skilled in the art without departing from the spirit of the present invention, and the technical solutions of the present invention are within the scope of the present invention defined by the claims.
Claims (6)
1. The feed for improving the birth weight of piglets and reducing the stillbirth rate is characterized in that the feed is a complete ration with the mass percentage of 99.9 percent and adenosine or adenosine monophosphate with the mass percentage of 0.1 percent; the total daily ration comprises the following components in percentage by weight: 34.68% of corn, 10% of rice bran meal, 16.67% of broken rice, 14.5% of soybean meal, 20% of triticale, 1.1% of stone powder, 0.96% of calcium bicarbonate, 0.1% of lysine, 0.05% of threonine, 0.4% of salt, 0.13% of choline chloride, 0.08% of mold remover, 0.05% of preservative and 1.18% of premix; the premix comprises the following components:vitamin A and vitamin D 3 Vitamin C, vitamin E, vitamin K, vitamin B 1 Vitamin B 2 Vitamin B 6 Vitamin B 12 Pantothenic acid, nicotinic acid, folic acid, biotin, copper, iron, zinc, manganese, iodine, and cobalt.
2. The feed according to claim 1, wherein the premix comprises the following components in an amount of 1kg of the full ration: vitamin A12000 IU and vitamin D 3 4800IU, vitamin C200mg, vitamin E205 mg, vitamin K3.6 mg, and vitamin B 1 3.6mg, vitamin B 2 12mg of vitamin B 6 7.2mg of vitamin B 12 0.048mg, pantothenic acid 30.0mg, nicotinic acid 48.0mg, folic acid 8.6mg, biotin 0.6mg, copper 10.0mg, iron 130mg, zinc 60mg, manganese 45mg, iodine 0.3mg, and cobalt 0.1mg.
3. Use of a feed according to any of claims 1-2 for increasing the birth weight of piglets.
4. The matched feeding method of the feed according to any one of claims 1-2, which is characterized by comprising the following steps:
(1) The feed intake of each sow is 2kg in 65-100 days of gestation;
(2) The daily feed intake of each sow from 100 days of gestation to 3 days before delivery is 2.5kg;
(3) The daily feed intake of each sow from 2 days before delivery to delivery was 2kg.
5. The matched feeding method according to claim 4, wherein the daily feed in the step (1) and the step (2) is fed at one time, and the daily feed in the step (3) is fed in 2 times.
6. The matched feeding method as claimed in claim 4, wherein the sow is a Du-Dagao three-way-breed primiparous sow.
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