CN114690370A

CN114690370A - Imaging lens assembly, microscope device and optical detection apparatus

Info

Publication number: CN114690370A
Application number: CN202011582981.7A
Authority: CN
Inventors: 冯子寅; 季敏标
Original assignee: Shanghai Junzhen Life Science Co ltd
Current assignee: Shanghai Junzhen Life Science Co ltd
Priority date: 2020-12-28
Filing date: 2020-12-28
Publication date: 2022-07-01
Anticipated expiration: 2040-12-28
Also published as: CN114690370B

Abstract

The present disclosure relates to an imaging mirror assembly, a microscope arrangement and an optical detection device. The imaging mirror assembly comprises: a first Gaussian lens group; a second Gaussian lens group; and the diaphragm is arranged between the first Gaussian lens group and the second Gaussian lens group and is configured to enable light within a preset distance from the optical axis of the image capturing lens component to pass through and block light outside the preset distance.

Description

Imaging lens assembly, microscope device and optical detection apparatus

Technical Field

The disclosure relates to the technical field of optical detection, in particular to an imaging lens assembly, a microscope device and optical detection equipment.

Background

Optical detection is increasingly used in the fields of chemistry, biology, and the like. In optical detection, bright field transmission imaging and/or dark field scattering imaging can be implemented to count particles and observe morphology (including size measurement, diameter distribution measurement, morphology observation, etc.) of biological particles (e.g., cells, cell debris, yeast, algae, etc.) in a sample. In addition, excitation light with a certain wavelength can be used for exciting a signal (such as a fluorescence signal) of the sample, so as to acquire related properties of the sample, and identify and analyze biological particles and the like. However, in the current imaging technology, under a low magnification, the obtained field of view is small, and the imaging focal length is large, so that the imaging effect of the related equipment is poor, and the related equipment has a large size, which brings inconvenience to detection.

Disclosure of Invention

It is an object of the present disclosure to provide an imaging mirror assembly, a microscopy apparatus and an optical detection device.

According to a first aspect of the present disclosure, there is provided an imaging mirror assembly comprising:

a first Gaussian lens group;

a second Gaussian lens group; and

a stop disposed between the first and second Gaussian lens groups and configured to pass light within a predetermined distance from an optical axis of the image capturing lens assembly and to block light outside the predetermined distance.

In some embodiments, the stop is disposed at a central position between the first and second gauss lens sets.

In some embodiments, the first and second gauss lens sets are symmetrically disposed about the stop.

In some embodiments, the first gaussian lens group comprises a first positive lens and a first negative lens group disposed adjacent to each other, wherein the first negative lens group is located between the first positive lens and the stop; and

the second gauss lens set comprises a second positive lens and a second negative lens set disposed adjacent to each other, wherein the second negative lens set is located between the diaphragm and the second positive lens.

In some embodiments, the first focal length of the first positive lens is equal to the second focal length of the second positive lens.

In some embodiments, the third focal length of the first negative lens group is equal to the fourth focal length of the second negative lens group.

In some embodiments, the first negative lens group comprises a first negative lens; and

the second negative lens group includes a second negative lens.

In some embodiments, the fifth focal length of the first negative lens is equal to the sixth focal length of the second negative lens.

In some embodiments, the first negative lens group includes a third positive lens and a third negative lens disposed adjacent to each other; and

the second negative lens group includes a fourth positive lens and a fourth negative lens disposed adjacent to each other.

In some embodiments, the third positive lens is disposed between the first positive lens and the third negative lens; and

the fourth positive lens is disposed between the fourth negative lens and the second positive lens.

In some embodiments, the seventh focal length of the third positive lens is equal to the eighth focal length of the fourth positive lens.

In some embodiments, the ninth focal length of the third negative lens is equal to the tenth focal length of the fourth negative lens.

In some embodiments, the focal length of the imaging mirror assembly is 50-80 mm.

According to a second aspect of the present disclosure, there is provided a microscopy apparatus comprising an imaging mirror assembly as described above.

In some embodiments, the microscopy apparatus further comprises:

a light source assembly configured to generate light for illuminating a sample;

the objective lens assembly is arranged between the light source assembly and the image forming lens assembly; and

the imaging assembly is arranged on an emergent light path of the imaging assembly;

wherein the objective lens assembly is configured to converge light generated by interaction with the sample into collimated light, and the imaging lens assembly is configured to converge the collimated light onto the imaging assembly.

In some embodiments, the distance between the image capture mirror assembly and the objective lens assembly is 50-120 mm.

According to a third aspect of the present disclosure, there is provided an optical detection apparatus comprising an imaging mirror assembly as described above or a microscopy device as described above.

Other features of the present disclosure and advantages thereof will become apparent from the following detailed description of exemplary embodiments thereof, which proceeds with reference to the accompanying drawings.

Drawings

The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments of the disclosure and together with the description, serve to explain the principles of the disclosure.

The present disclosure may be more clearly understood from the following detailed description, taken with reference to the accompanying drawings, in which:

FIG. 1 shows a schematic structural view of an imaging mirror assembly;

FIG. 2 shows an imaging field-of-view photograph on an 2/3 inch imaging assembly using a 5-fold objective lens assembly and the imaging lens assembly of FIG. 1;

FIG. 3 shows an imaging field of view photograph on a 1 inch imaging assembly using a 5-fold objective lens assembly and the imaging lens assembly of FIG. 1;

fig. 4 shows a schematic structural diagram of an imaging mirror assembly according to an exemplary embodiment of the present disclosure;

fig. 5 shows a schematic structural view of an imaging mirror assembly according to another exemplary embodiment of the present disclosure;

FIG. 6 shows an optical path diagram of the imaging mirror assembly of FIG. 5;

FIG. 7 shows an imaging field-of-view photograph on an 2/3 inch imaging assembly using a 5-fold objective lens assembly and the imaging lens assembly of FIG. 5;

FIG. 8 shows an imaging field of view photograph on a 1 inch imaging assembly using a 5-fold objective lens assembly and the imaging lens assembly of FIG. 5;

fig. 9 shows a schematic structural diagram of a microscopy apparatus according to an exemplary embodiment of the present disclosure.

Note that in the embodiments described below, the same reference numerals are used in common between different drawings to denote the same portions or portions having the same functions, and a repetitive description thereof will be omitted. In this specification, like reference numerals and letters are used to designate like items, and therefore, once an item is defined in one drawing, further discussion thereof is not required in subsequent drawings.

For convenience of understanding, the positions, sizes, ranges, and the like of the respective structures shown in the drawings and the like do not sometimes indicate actual positions, sizes, ranges, and the like. Therefore, the disclosed invention is not limited to the positions, dimensions, ranges, etc., disclosed in the drawings and the like. Furthermore, the figures are not necessarily to scale, some features may be exaggerated to show details of particular components.

Detailed Description

Various exemplary embodiments of the present disclosure will now be described in detail with reference to the accompanying drawings. It should be noted that: the relative arrangement of the components and steps, the numerical expressions, and numerical values set forth in these embodiments do not limit the scope of the present disclosure unless specifically stated otherwise.

The following description of at least one exemplary embodiment is merely illustrative in nature and is in no way intended to limit the disclosure, its application, or uses. That is, the chip testing method and the computing chip herein are shown by way of example to illustrate different embodiments of the circuit or method in the present disclosure and are not intended to be limiting. Those skilled in the art will appreciate that they are merely illustrative of ways that the invention may be practiced, not exhaustive.

Techniques, methods, and apparatus known to those of ordinary skill in the relevant art may not be discussed in detail but are intended to be part of the specification where appropriate.

Fig. 1 is a schematic diagram of a conventional image capture mirror assembly 100'. The imaging mirror assembly 100 ' includes a double cemented lens, wherein the double cemented lens may specifically include a convex lens 110 ', a concave lens 120 ', a convex lens 130 ', and a concave lens 140 ' arranged in sequence. In the imaging mirror assembly 100 ' shown in fig. 1, the convex lens 110 ' and the concave lens 120 ' are disposed in close contact with each other, and the convex lens 130 ' and the concave lens 140 ' are disposed in close contact with each other. The curvature of the surface of the convex lens 110 ' that conforms to the concave lens 120 ' may be matched to the curvature of the corresponding surface of the concave lens 120 ' to facilitate the gluing. Similarly, the curvature of the surface of the convex lens 130 ' that conforms to the concave lens 140 ' may be matched to the curvature of the corresponding surface of the concave lens 140 ' to facilitate the gluing. The concave lens 120 'and the convex lens 130' may be separately provided to obtain a desired imaging effect. By selecting the relevant parameters of the convex lens 110 ', the concave lens 120', the convex lens 130 'and the concave lens 140', the image capturing mirror assembly 100 'can have smaller spherical aberration and chromatic aberration, so that the image capturing mirror assembly 100' can have better imaging effect when used with an objective lens assembly. As shown in fig. 2 and 3, are photographs of the imaging field of view on an 2/3 inch imaging assembly using a 5-fold objective lens assembly and the imaging lens assembly of fig. 1, respectively. As can be seen in fig. 2 and 3, when such a junction mirror assembly 100' is used, the corresponding imaging field of view is small and may cause much inconvenience to the inspection process. In addition, such an imaging lens assembly 100 ' generally has a longer focal length, for example, 180-200 mm, resulting in a larger volume of the imaging lens assembly 100 ' and other devices, apparatuses, etc. using the imaging lens assembly 100 ', which is not convenient to use.

According to the exemplary embodiment of the present disclosure, a new imaging mirror assembly is provided, which adopts a double-gauss structure, and can achieve a larger imaging field of view and a shorter focal length, thereby facilitating a reduction in the volume of the imaging mirror assembly and other devices, apparatuses, etc. using the imaging mirror assembly, improving the imaging effect, and facilitating optical detection at low magnification.

As shown in fig. 4 and 5, in an exemplary embodiment of the present disclosure, the objective lens assembly 100 may include a first gauss lens group 110, a second gauss lens group 120, and a stop 130 disposed between the first gauss lens group 110 and the second gauss lens group 120. The imaging mirror assembly 100 may converge parallel or nearly parallel imaging beams generated by an objective lens assembly (not shown) at a predetermined position (e.g., an imaging position of the imaging assembly) to achieve imaging.

The double-gauss structure can have a variety of different arrangements to achieve the desired imaging effect.

In some embodiments, as shown in fig. 4 and 5, the first and second

gauss lens groups

110 and 120 may be symmetrically disposed about the stop 130. In one aspect, the distance between the first gauss lens set 110 and the stop 130 may be equal to the distance between the second gauss lens set 120 and the stop 130. In other words, the stop 130 may be disposed at a central position between the first and second

gauss lens groups

110 and 120. The plane in which the shading screen of the diaphragm 130 lies is generally perpendicular to the optical axis. On the other hand, the corresponding geometric parameters and optical parameters between the first and second gauss lens sets 110 and 120 may be the same or substantially the same. For example, the focal length of the first gauss lens set 110 may be equal to the focal length of the second gauss lens set 120. In the first gauss lens group 110, the refractive index, the abbe number, the size of the lens, the focal length of the lens, the positional relationship between different lenses, etc. of the material forming each lens therein may also be correspondingly the same or substantially the same as the refractive index, the abbe number, the size of the lens, the focal length of the lens, the positional relationship between different lenses, etc. of the material forming each lens in the second gauss lens group 120.

In a gaussian structure, a first positive lens group and a second positive lens group are generally included, which are disposed adjacent to each other, to achieve a desired imaging effect. As shown in fig. 4 and 5, the first gaussian lens group 110 may include a first positive lens 111 and a first negative lens group disposed adjacent to each other, wherein the first negative lens group may be located between the first positive lens 111 and the stop 130.

As shown in fig. 6, the stop 130 disposed between the first and second

gauss lens groups

110 and 120 may be configured to pass light within a preset distance from the optical axis of the image capturing mirror assembly and block light outside the preset distance, thereby eliminating stray light and improving imaging effect. A beam passing through a double gaussian structure can form a larger imaging field of view at the imaging location. The size of the imaging field of view can also be adjusted by changing the size of the preset distance, i.e. changing the size of the clear aperture of the diaphragm 130.

In some embodiments, the first positive lens 111 may be a meniscus type positive lens. In other embodiments, the first positive lens 111 may also be a plano-convex lens or a positive lens close to a plano-convex lens.

The first negative lens group may include one or more lenses. For example, in an exemplary embodiment shown in fig. 4, the first negative lens group may include only the first negative lens 112, and the first negative lens 112 may be a meniscus negative lens, a plano-concave lens, or a negative lens close to a plano-concave lens. In an exemplary embodiment shown in fig. 5, the first negative lens group may include a third positive lens 113 and a third negative lens 114 disposed adjacent to each other. Among them, the third positive lens 113 may be disposed between the first positive lens 111 and the third negative lens 114. The focal lengths of the third positive lens 113 and the third negative lens 114 are selected so that the entirety thereof has a negative lens action on the light beam. In some embodiments, the third positive lens 113 may be a double convex lens or a meniscus convex lens, the third negative lens 114 may be a double concave lens or a meniscus concave lens, and one curved surface of the third positive lens 113 and one curved surface of the third negative lens 114 are attached to each other. In other embodiments, the third positive lens 113 may be a plano-convex lens, the third negative lens 114 may be a plano-concave lens, and the plane of the third positive lens 113 and the plane of the third negative lens 114 may be attached to each other.

The six-piece double-gauss structure shown in fig. 5 can reduce a larger interval between the first positive lens 111 and the lens of the first negative lens group, thereby reducing corresponding aberrations, as compared to the first negative lens group including only one negative lens shown in fig. 4. In some embodiments, the third positive lens 113 and the third negative lens 114 can be made of transparent materials with different abbe numbers and substantially the same refractive index, so as to further eliminate aberration, and thus, have high-quality imaging effect under the condition of large aperture.

Similarly, as shown in fig. 4 and 5, the second gauss lens set 120 may include a second positive lens 121 and a second negative lens set disposed adjacent to each other, wherein the second negative lens set is located between the diaphragm 130 and the second positive lens 121.

In some embodiments, the second positive lens 121 may be a meniscus-type positive lens. In other embodiments, the second positive lens 121 may also be a plano-convex lens or a positive lens close to a plano-convex lens.

The second negative lens group may also include one or more lenses. For example, in an exemplary embodiment shown in fig. 4, the second negative lens group may include only the second negative lens 122, and the second negative lens 122 may be a meniscus negative lens, a plano-concave lens, or a negative lens close to a plano-concave lens. In an exemplary embodiment shown in fig. 5, the second negative lens group may include a fourth positive lens 123 and a fourth negative lens 124 disposed adjacent to each other. Wherein the fourth positive lens 123 may be disposed between the fourth negative lens 124 and the second positive lens 121. The focal lengths of the fourth positive lens 123 and the fourth negative lens 124 are selected such that the entirety thereof has a negative lens action on the light beam. In some embodiments, the fourth positive lens 123 may be a double convex lens or a meniscus convex lens, the fourth negative lens 124 may be a double concave lens or a meniscus concave lens, and one curved surface of the fourth positive lens 123 and one curved surface of the fourth negative lens 124 are attached to each other. In other embodiments, the fourth positive lens 123 may be a plano-convex lens, the fourth negative lens 124 may be a plano-concave lens, and the plane of the fourth positive lens 123 and the plane of the fourth negative lens 124 may be attached to each other.

Also, the six-piece double-gauss structure shown in fig. 5 can reduce a larger interval between the second positive lens 121 and the lens pieces of the second negative lens group, as compared to the first negative lens group including only one negative lens shown in fig. 4, thereby reducing the corresponding aberrations. In some embodiments, the fourth positive lens 123 and the fourth negative lens 124 can be made of transparent materials with different abbe numbers and substantially the same refractive index, so as to further eliminate aberration, and thus, have high-quality imaging effect even under a large aperture.

As can be seen from the above description, the first and second gauss lens sets 110 and 120 can be symmetrically disposed. That is, in the embodiment shown in fig. 4, the refractive index, the abbe number, the size of the lens, the focal length of the lens, and other parameters of the materials of the first positive lens 111 and the second positive lens 121 may be correspondingly equal, the refractive index, the abbe number, the size of the lens, the focal length of the lens, and other parameters of the materials of the first negative lens 112 and the first negative lens 122 may be correspondingly equal, and the positions of the first positive lens 111 and the second positive lens 121 are symmetrical with respect to the diaphragm 130, and the positions of the first negative lens 112 and the second negative lens 122 are symmetrical with respect to the diaphragm 130. In the embodiment shown in fig. 5, the refractive index, the abbe number, the size of the lens, the focal length of the lens, and other parameters of the materials of the first positive lens 111 and the second positive lens 121 may be correspondingly equal, the refractive index, the abbe number, the size of the lens, the focal length of the lens, and other parameters of the materials of the third positive lens 113 and the fourth positive lens 123 may be correspondingly equal, the refractive index, the abbe number, the size of the lens, the focal length of the lens, and other parameters of the materials of the third negative lens 114 and the fourth negative lens 124 may be correspondingly equal, and the positions of the first positive lens 111 and the second positive lens 121 are symmetrical with respect to the diaphragm 130, the positions of the third positive lens 113 and the fourth positive lens 123 are symmetrical with respect to the diaphragm 130, and the positions of the third negative lens 114 and the fourth negative lens 124 are symmetrical with respect to the diaphragm 130.

Of course, in other embodiments, the first and second gauss lens sets can also be asymmetrically disposed. In the asymmetrically arranged double-gaussian structure, the aberration in the symmetrical double-gaussian structure can be further corrected.

As shown in fig. 7 and 8 are photographs of the imaging field on 2/3 inch and 1 inch imaging assemblies using a 5-fold objective lens assembly and the imaging lens assembly of fig. 5, respectively. The imaging field of view in fig. 7 and 8 is significantly increased, e.g., the diameter of the imaging field of view may be increased to more than 150% of the original diameter, as compared to the imaging field of view in fig. 2 and 3 using the imaging mirror assembly 100' of fig. 1.

In addition, by selecting the relevant parameters of the first gauss lens set 110 and the second gauss lens set 120, the focal length of the image capturing mirror assembly 100 can be in the range of 50-80 mm, which is greatly reduced compared with the image capturing mirror assembly 100' shown in fig. 1, so that the volume of the image capturing mirror assembly and the device, equipment and the like using the image capturing mirror assembly can be reduced for convenient use.

The present disclosure also contemplates a microscopy apparatus, as shown in fig. 9, which may include an imaging mirror assembly 100 as described above.

In addition, as shown in fig. 9, the microscope apparatus may further include a light source assembly 200, an objective lens assembly 300, and an imaging assembly 400, wherein arrows indicate the propagation direction of light beams. Therein, the light source assembly 200 may be configured to generate light for illuminating the sample 900. The objective lens assembly 300 may be disposed between the light source assembly 200 and the image forming lens assembly 100 to converge light generated by interaction with the sample 900 into parallel or near-parallel light. The imaging assembly 400 may be positioned in the path of the exit light of the imaging mirror assembly 100 such that the imaging mirror assembly 100 may focus collimated or nearly collimated light onto the imaging assembly 400. The imaging assembly 400 may include a Charge Coupled Device (CCD) or a complementary metal oxide semiconductor device (CMOS). The distance between the objective lens assembly 100 and the objective lens assembly 300 may be calculated primarily from the Numerical Aperture (NA) of the objective lens assembly 300, the stop diameter of the exit pupil, the exit pupil angle, and the diameter of the objective lens assembly 100. In some embodiments, the distance between the objective lens assembly 300 and the image capture lens assembly 100 may be 50-120 mm.

Furthermore, the present disclosure also proposes an optical detection device, which may comprise an imaging mirror assembly or a microscopy apparatus as described above. In the optical detection device, illumination light or excitation light generated by the light source assembly can be projected onto a sample at the sample stage to realize illumination of the sample so as to perform optical observation; or exciting a signal such as fluorescence of the sample, and analyzing the relevant property of the sample according to the acquired signal. In addition, in some embodiments, the optical inspection device may further include a memory, processor, or the like communicatively coupled to the imaging assembly to automatically store and process image information formed by the imaging assembly to further facilitate inspection.

In all examples shown and discussed herein, any particular value should be construed as exemplary only and not as limiting. Thus, other examples of the exemplary embodiments may have different values.

The terms "front," "back," "top," "bottom," "over," "under," and the like in the description and in the claims, if any, are used for descriptive purposes and not necessarily for describing permanent relative positions. It is to be understood that the terms so used are interchangeable under appropriate circumstances such that the embodiments of the disclosure described herein are, for example, capable of operation in other orientations than those illustrated or otherwise described herein.

As used herein, the word "exemplary" means "serving as an example, instance, or illustration," and not as a "model" that is to be replicated accurately. Any implementation exemplarily described herein is not necessarily to be construed as preferred or advantageous over other implementations. Furthermore, the disclosure is not limited by any expressed or implied theory presented in the preceding technical field, background, brief summary or the detailed description.

As used herein, the term "substantially" is intended to encompass any minor variation resulting from design or manufacturing imperfections, device or component tolerances, environmental influences, and/or other factors. The word "substantially" also allows for differences from a perfect or ideal situation due to parasitic effects, noise, and other practical considerations that may exist in a practical implementation.

The above description may indicate elements or nodes or features being "connected" or "coupled" together. As used herein, unless expressly stated otherwise, "connected" means that one element/node/feature is directly connected to (or directly communicates with) another element/node/feature, either electrically, mechanically, logically, or otherwise. Similarly, unless expressly stated otherwise, "coupled" means that one element/node/feature may be mechanically, electrically, logically or otherwise joined to another element/node/feature in a direct or indirect manner to allow interaction, even though the two features may not be directly connected. That is, coupled is intended to include both direct and indirect joining of elements or other features, including connection with one or more intermediate elements.

It will be further understood that the terms "comprises/comprising," "includes" and/or "including," when used herein, specify the presence of stated features, integers, steps, operations, elements, and/or components, but do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, and/or groups thereof.

Those skilled in the art will appreciate that the boundaries between the above described operations merely illustrative. Multiple operations may be combined into a single operation, single operations may be distributed in additional operations, and operations may be performed at least partially overlapping in time. Moreover, alternative embodiments may include multiple instances of a particular operation, and the order of operations may be altered in various other embodiments. However, other modifications, variations, and alternatives are also possible. The specification and drawings are, accordingly, to be regarded in an illustrative rather than a restrictive sense.

Although some specific embodiments of the present disclosure have been described in detail by way of example, it should be understood by those skilled in the art that the foregoing examples are for purposes of illustration only and are not intended to limit the scope of the present disclosure. The various embodiments disclosed herein may be combined in any combination without departing from the spirit and scope of the present disclosure. It will also be appreciated by those skilled in the art that various modifications may be made to the embodiments without departing from the scope and spirit of the disclosure. The scope of the present disclosure is defined by the appended claims.

Claims

1. An imaging mirror assembly, comprising:

a first Gaussian lens group;

a second Gaussian lens group; and

2. An image capture mirror assembly according to claim 1, wherein said stop is disposed centrally between said first and second gauss lens sets.

3. The imaging mirror assembly of claim 1, wherein said first and second gauss lens sets are symmetrically disposed about said stop.

4. An imaging mirror assembly according to claim 1, wherein said first gauss lens set comprises a first positive lens and a first negative lens set disposed adjacent to each other, wherein said first negative lens set is located between said first positive lens and said diaphragm; and

5. The imaging mirror assembly of claim 4, wherein said first positive lens has a first focal length equal to a second focal length of said second positive lens.

6. The imaging mirror assembly of claim 4, wherein said third focal length of said first negative lens group is equal to said fourth focal length of said second negative lens group.

7. The imaging mirror assembly of claim 4, wherein said first negative lens group comprises a first negative lens; and

the second negative lens group includes a second negative lens.

8. The imaging mirror assembly of claim 7, wherein said first negative lens has a fifth focal length equal to a sixth focal length of said second negative lens.

9. A microscopy apparatus comprising an imaging mirror assembly according to any one of claims 1 to 8.

10. An optical detection device, characterized in that it comprises an imaging mirror assembly according to any one of claims 1 to 8 or a microscopy apparatus according to claim 9.