CN114690370B

CN114690370B - Imaging lens assembly, microscopic device and optical detection equipment

Info

Publication number: CN114690370B
Application number: CN202011582981.7A
Authority: CN
Inventors: 冯子寅; 季敏标
Original assignee: Shanghai Junzhen Life Science Co ltd
Current assignee: Shanghai Junzhen Life Science Co ltd
Priority date: 2020-12-28
Filing date: 2020-12-28
Publication date: 2023-06-23
Anticipated expiration: 2040-12-28
Also published as: CN114690370A

Abstract

The present disclosure relates to an imaging mirror assembly, a microscopy apparatus, and an optical detection apparatus. The imaging mirror assembly includes: a first Gaussian lens group; a second Gaussian lens group; and a diaphragm disposed between the first Gaussian lens group and the second Gaussian lens group, the diaphragm being configured to pass light within a preset distance from an optical axis of the imaging lens assembly and to block light outside the preset distance.

Description

Imaging lens assembly, microscopic device and optical detection equipment

Technical Field

The disclosure relates to the technical field of optical detection, and in particular relates to an imaging lens assembly, a microscopic device and optical detection equipment.

Background

In the fields of chemistry, biology, etc., optical detection is increasingly used. In optical detection, bright field transmission imaging and/or dark field scattering imaging can be implemented to perform particle counting and morphology observation (including size measurement, diameter distribution measurement, morphology observation, etc.) of biological particles (e.g., cells, cell debris, yeast, algae, etc.) and the like in a sample. In addition, a signal (for example, a fluorescent signal) of the sample may be excited by using excitation light having a certain wavelength, so that the relevant property of the sample is obtained, and the biological particles and the like are identified and analyzed. However, in the current imaging technology, under the condition of low magnification, the obtained field of view is smaller, the imaging focal length is larger, so that the imaging effect of related equipment is poor, the related equipment has larger size, and inconvenience is brought to detection.

Disclosure of Invention

It is an object of the present disclosure to provide an imaging mirror assembly, a microscopy apparatus and an optical detection apparatus.

According to a first aspect of the present disclosure, there is provided an imaging mirror assembly comprising:

a first Gaussian lens group;

a second Gaussian lens group; and

and the diaphragm is arranged between the first Gaussian lens group and the second Gaussian lens group, and is configured to enable light which is within a preset distance from the optical axis of the imaging lens assembly to pass through and block light which is outside the preset distance.

In some embodiments, the stop is disposed at a central location between the first and second gaussian lens sets.

In some embodiments, the first gaussian lens group and the second gaussian lens group are symmetrically disposed about the stop.

In some embodiments, the first gaussian lens group comprises a first positive lens and a first negative lens group disposed adjacent to each other, wherein the first negative lens group is located between the first positive lens and the stop; and

the second Gaussian lens group includes a second positive lens and a second negative lens group disposed adjacent to each other, wherein the second negative lens group is located between the diaphragm and the second positive lens.

In some embodiments, the first focal length of the first positive lens is equal to the second focal length of the second positive lens.

In some embodiments, the third focal length of the first negative lens group is equal to the fourth focal length of the second negative lens group.

In some embodiments, the first negative lens group includes a first negative lens; and

the second negative lens group includes a second negative lens.

In some embodiments, the fifth focal length of the first negative lens is equal to the sixth focal length of the second negative lens.

In some embodiments, the first negative lens group includes a third positive lens and a third negative lens disposed adjacent to each other; and

the second negative lens group includes a fourth positive lens and a fourth negative lens disposed adjacent to each other.

In some embodiments, the third positive lens is disposed between the first positive lens and the third negative lens; and

the fourth positive lens is arranged between the fourth negative lens and the second positive lens.

In some embodiments, the seventh focal length of the third positive lens is equal to the eighth focal length of the fourth positive lens.

In some embodiments, the ninth focal length of the third negative lens is equal to the tenth focal length of the fourth negative lens.

In some embodiments, the focal length of the imaging mirror assembly is 50-80 mm.

According to a second aspect of the present disclosure there is provided a microscopy apparatus comprising an imaging mirror assembly as described above.

In some embodiments, the microscopy apparatus further comprises:

a light source assembly configured to generate light for illuminating a sample;

the objective lens assembly is arranged between the light source assembly and the imaging lens assembly; and

the imaging component is arranged on an emergent light path of the imaging mirror component;

wherein the objective lens assembly is configured to focus light generated by interaction with the sample into parallel light, and the imaging lens assembly is configured to focus the parallel light onto the imaging assembly.

In some embodiments, the distance between the imaging mirror assembly and the objective lens assembly is 50-120 mm.

According to a third aspect of the present disclosure, there is provided an optical detection apparatus comprising an imaging mirror assembly as described above or a microscopy device as described above.

Other features of the present disclosure and its advantages will become apparent from the following detailed description of exemplary embodiments of the disclosure, which proceeds with reference to the accompanying drawings.

Drawings

The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments of the disclosure and together with the description, serve to explain the principles of the disclosure.

The disclosure may be more clearly understood from the following detailed description taken in conjunction with the accompanying drawings in which:

FIG. 1 shows a schematic structural view of an imaging mirror assembly;

FIG. 2 shows a photograph of an imaging field of view on a 2/3 inch imaging assembly using a 5 x objective lens assembly and the imaging lens assembly of FIG. 1;

FIG. 3 shows a photograph of an imaging field of view on a 1 inch imaging assembly using a 5 x objective lens assembly and the imaging lens assembly of FIG. 1;

FIG. 4 illustrates a schematic structural diagram of an imaging mirror assembly according to an exemplary embodiment of the present disclosure;

FIG. 5 illustrates a schematic structural view of an imaging mirror assembly according to another exemplary embodiment of the present disclosure;

FIG. 6 illustrates an optical path diagram of the imaging mirror assembly of FIG. 5;

FIG. 7 shows a photograph of an imaging field of view on a 2/3 inch imaging assembly using a 5 x objective lens assembly and the imaging lens assembly of FIG. 5;

FIG. 8 shows a photograph of an imaging field of view on a 1 inch imaging assembly using a 5 x objective lens assembly and the imaging lens assembly of FIG. 5;

fig. 9 shows a schematic structural view of a microscopy apparatus according to an exemplary embodiment of the disclosure.

Note that in the embodiments described below, the same reference numerals are used in common between different drawings to denote the same parts or parts having the same functions, and a repetitive description thereof may be omitted. In this specification, like reference numerals and letters are used to designate like items, and thus once an item is defined in one drawing, no further discussion thereof is necessary in subsequent drawings.

For ease of understanding, the positions, dimensions, ranges, etc. of the respective structures shown in the drawings and the like may not represent actual positions, dimensions, ranges, etc. Accordingly, the disclosed invention is not limited to the disclosed positions, dimensions, ranges, etc. as illustrated in the drawings. Moreover, the figures are not necessarily to scale, some features may be exaggerated to show details of particular components.

Detailed Description

Various exemplary embodiments of the present disclosure will now be described in detail with reference to the accompanying drawings. It should be noted that: the relative arrangement of the components and steps, numerical expressions and numerical values set forth in these embodiments do not limit the scope of the present disclosure unless it is specifically stated otherwise.

The following description of at least one exemplary embodiment is merely illustrative in nature and is in no way intended to limit the disclosure, its application, or uses. That is, the chip test methods and computing chips herein are shown by way of example to illustrate different embodiments of the circuits or methods in this disclosure, and are not intended to be limiting. Those skilled in the art will appreciate that they are merely illustrative of exemplary ways in which the invention may be practiced, and not exhaustive.

Techniques, methods, and apparatus known to one of ordinary skill in the relevant art may not be discussed in detail, but should be considered part of the specification where appropriate.

Fig. 1 is a schematic diagram of a conventional imaging mirror assembly 100'. The image mirror assembly 100' includes a doublet lens, wherein the doublet lens may include, in particular, a convex lens 110', a concave lens 120', a convex lens 130', and a concave lens 140' arranged in that order. In the image mirror assembly 100' shown in fig. 1, the convex lens 110' and the concave lens 120' are disposed to be attached to each other, and the convex lens 130' and the concave lens 140' are disposed to be attached to each other. The curvature of the surface of the convex lens 110' that is fitted with the concave lens 120' may be consistent with the curvature of the corresponding surface of the concave lens 120' to facilitate the gluing. Similarly, the curvature of the surface of the convex lens 130' that conforms to the concave lens 140' may be consistent with the curvature of the corresponding surface of the concave lens 140' to facilitate bonding. The concave lens 120 'and the convex lens 130' may be separately provided to obtain a desired imaging effect. By selecting the parameters of the convex lens 110', the concave lens 120', the convex lens 130 'and the concave lens 140', the image lens assembly 100 'can have smaller spherical aberration and chromatic aberration, so that the image lens assembly 100' can be matched with an objective lens assembly for use, and has better imaging effect. As shown in fig. 2 and 3, photographs of the imaging field of view on a 2/3 inch imaging assembly using a 5 x objective lens assembly and the imaging lens assembly of fig. 1, respectively. As can be seen from fig. 2 and 3, when such an imaging mirror assembly 100' is used, the corresponding imaging field of view is small, which may cause a lot of inconvenience to the detection process. In addition, such an imaging lens assembly 100' typically has a long focal length, e.g., 180-200 mm, resulting in a large volume of the imaging lens assembly 100' and other devices, equipment, etc. in which the imaging lens assembly 100' is used, which is not convenient.

According to the exemplary embodiments of the present disclosure, a new imaging mirror assembly is provided, which adopts a double gaussian structure, and can achieve a larger imaging field of view and a shorter focal length, thereby helping to reduce the volume of the imaging mirror assembly and other devices, apparatuses, etc. using the imaging mirror assembly, improving the imaging effect, and facilitating optical detection under low magnification.

As shown in fig. 4 and 5, in an exemplary embodiment of the present disclosure, the imaging lens assembly 100 may include a first gaussian lens group 110, a second gaussian lens group 120, and a diaphragm 130 disposed between the first gaussian lens group 110 and the second gaussian lens group 120. The imaging mirror assembly 100 may converge parallel or near-parallel imaging beams generated by an objective lens assembly (not shown) at a predetermined location (e.g., an imaging location of the imaging assembly) to achieve imaging.

The double gaussian structure may have a number of different arrangements to achieve the desired imaging effect.

In some embodiments, as shown in fig. 4 and 5, the first gaussian lens group 110 and the second gaussian lens group 120 may be symmetrically disposed about the stop 130. In one aspect, the distance between the first gaussian lens set 110 and the stop 130 may be equal to the distance between the second gaussian lens set 120 and the stop 130. In other words, the diaphragm 130 may be disposed at a central position between the first gaussian lens group 110 and the second gaussian lens group 120. The plane of the shading of the diaphragm 130 is generally perpendicular to the optical axis. On the other hand, the corresponding geometric and optical parameters between the first and second gaussian lens sets 110, 120 may be identical or substantially identical. For example, the focal length of the first gaussian lens set 110 may be equal to the focal length of the second gaussian lens set 120. In the first gaussian lens group 110, the refractive index, the abbe number, the size of the lens, the focal length of the lens, the positional relationship between different lenses, and the like of the material forming each lens therein may also be the same as or substantially the same as the refractive index, the abbe number, the size of the lens, the focal length of the lens, the positional relationship between different lenses, and the like of the material of each lens in the second gaussian lens group 120.

In one gaussian structure, a first positive lens group and a second positive lens group are generally included that are disposed adjacent to each other to achieve a desired imaging effect. As shown in fig. 4 and 5, the first gaussian lens group 110 may include a first positive lens 111 and a first negative lens group disposed adjacent to each other, wherein the first negative lens group may be located between the first positive lens 111 and the stop 130.

As shown in fig. 6, the diaphragm 130 disposed between the first and second

gaussian lens groups

110 and 120 may be configured to pass light within a preset distance from the optical axis of the imaging lens assembly and block light outside the preset distance, thereby eliminating stray light and improving imaging effects. A beam passing through the double gaussian structure can form a larger imaging field of view at the imaging location. The size of the imaging field of view can also be adjusted to some extent by changing the size of the preset distance, i.e., the size of the clear aperture of the stop 130.

In some embodiments, the first positive lens 111 may be a meniscus positive lens. In other embodiments, the first positive lens 111 may also be a plano-convex lens or a positive lens that approximates a plano-convex lens.

The first negative lens group may include one or more lenses. For example, in an exemplary embodiment shown in fig. 4, the first negative lens group may include only the first negative lens 112, and the first negative lens 112 may be a crescent-shaped negative lens, or may be a plano-concave lens or a negative lens close to a plano-concave lens. In an exemplary embodiment shown in fig. 5, the first negative lens group may include a third positive lens 113 and a third negative lens 114 disposed adjacent to each other. Wherein the third positive lens 113 may be disposed between the first positive lens 111 and the third negative lens 114. The focal lengths of the third positive lens 113 and the third negative lens 114 are selected so that the whole thereof has a negative lens effect on the light beam. In some embodiments, the third positive lens 113 may be a biconvex lens or a crescent-type convex lens, the third negative lens 114 may be a biconcave lens or a crescent-type concave lens, and one curved surface of the third positive lens 113 and one curved surface of the third negative lens 114 are attached to each other. In other embodiments, the third positive lens 113 may be a plano-convex lens, the third negative lens 114 may be a plano-concave lens, and the plane of the third positive lens 113 and the plane of the third negative lens 114 may be attached to each other.

The six-piece double-gauss structure shown in fig. 5 can reduce a larger interval between the first positive lens 111 and the lenses of the first negative lens group, thereby reducing the corresponding aberration, compared to the first negative lens group including only one negative lens shown in fig. 4. In some embodiments, the third positive lens 113 and the third negative lens 114 may also be made of transparent materials having different abbe numbers and substantially the same refractive index, thereby further eliminating aberrations, which may also have a high quality imaging effect in the case of a large aperture.

Similarly, as shown in fig. 4 and 5, the second gaussian lens group 120 may include a second positive lens 121 and a second negative lens group disposed adjacent to each other, wherein the second negative lens group is located between the stop 130 and the second positive lens 121.

In some embodiments, the second positive lens 121 may be a meniscus positive lens. In other embodiments, the second positive lens 121 may also be a plano-convex lens or a positive lens that approximates a plano-convex lens.

The second negative lens group may also include one or more lenses. For example, in an exemplary embodiment shown in fig. 4, the second negative lens group may include only the second negative lens 122, and the second negative lens 122 may be a crescent-shaped negative lens, or may be a plano-concave lens or a negative lens close to a plano-concave lens. In an exemplary embodiment shown in fig. 5, the second negative lens group may include a fourth positive lens 123 and a fourth negative lens 124 disposed adjacent to each other. Wherein the fourth positive lens 123 may be disposed between the fourth negative lens 124 and the second positive lens 121. The focal lengths of the fourth positive lens 123 and the fourth negative lens 124 are selected so that the whole thereof has a negative lens effect on the light beam. In some embodiments, the fourth positive lens 123 may be a biconvex lens or a crescent-shaped convex lens, the fourth negative lens 124 may be a biconcave lens or a crescent-shaped concave lens, and one curved surface of the fourth positive lens 123 and one curved surface of the fourth negative lens 124 are attached to each other. In other embodiments, the fourth positive lens 123 may be a plano-convex lens, the fourth negative lens 124 may be a plano-concave lens, and the plane of the fourth positive lens 123 and the plane of the fourth negative lens 124 may be attached to each other.

Also, the six-piece double-gauss structure shown in fig. 5 can reduce a larger interval between the second positive lens 121 and the lenses of the second negative lens group, thereby reducing the corresponding aberration, compared to the first negative lens group including only one negative lens shown in fig. 4. In some embodiments, the fourth positive lens 123 and the fourth negative lens 124 may also be made of transparent materials having different abbe numbers and substantially the same refractive index, thereby further eliminating aberrations, which may also have a high quality imaging effect in the case of a large aperture.

As can be seen from the above description, the first gaussian lens unit 110 and the second gaussian lens unit 120 may be symmetrically arranged. That is, in the embodiment shown in fig. 4, the refractive index, the dispersion coefficient, the size of the lens, the focal length of the lens, and the like of the materials of the first positive lens 111 and the second positive lens 121 may be correspondingly equal, the refractive index, the dispersion coefficient, the size of the lens, the focal length of the lens, and the like of the materials of the first negative lens 112 and the first negative lens 122 may be correspondingly equal, and the positions of the first positive lens 111 and the second positive lens 121 are symmetrical with respect to the diaphragm 130, and the positions of the first negative lens 112 and the second negative lens 122 are symmetrical with respect to the diaphragm 130. In the embodiment shown in fig. 5, the refractive index, the dispersion coefficient, the size of the lens, the focal length of the lens, and the like of the materials of the first positive lens 111 and the second positive lens 121 may be correspondingly equal, the refractive index, the dispersion coefficient, the size of the lens, the focal length of the lens, and the like of the materials of the third positive lens 113 and the fourth positive lens 123 may be correspondingly equal, the refractive index, the dispersion coefficient, the size of the lens, the focal length of the lens, and the like of the materials of the third negative lens 114 and the fourth negative lens 124 may be correspondingly equal, and the positions of the first positive lens 111 and the second positive lens 121 are symmetrical with respect to the aperture 130, the positions of the third positive lens 113 and the fourth positive lens 123 are symmetrical with respect to the aperture 130, and the positions of the third negative lens 114 and the fourth negative lens 124 are symmetrical with respect to the aperture 130.

Of course, in other embodiments, the first gaussian lens set and the second gaussian lens set may be asymmetrically disposed. In the asymmetric double gaussian structure, aberrations in the symmetric double gaussian structure can be further corrected.

Photographs of imaging fields of view on 2/3 inch and 1 inch imaging assemblies using a 5 x objective lens assembly and the imaging lens assembly of fig. 5 are shown in fig. 7 and 8, respectively. The imaging field of view in fig. 7 and 8 is significantly increased compared to the imaging field of view in fig. 2 and 3 using the imaging mirror assembly 100' of fig. 1, e.g., the diameter of the imaging field of view may be increased above 150% of the original.

In addition, by selecting the relevant parameters of the first and second

gaussian lens groups

110 and 120, the focal length of the image mirror assembly 100 can be in the range of 50 to 80mm, which is greatly reduced compared to the image mirror assembly 100' shown in fig. 1, so that the volumes of the image mirror assembly and the devices, apparatuses, etc. using the same can be reduced for convenience of use.

The present disclosure also contemplates a microscopy apparatus, as shown in fig. 9, that may include an imaging mirror assembly 100 as described above.

In addition, as shown in fig. 9, the microscopic device may further include a light source assembly 200, an objective lens assembly 300, and an imaging assembly 400, the arrows shown in the drawing indicate the propagation directions of the light beams. Wherein the light source assembly 200 may be configured to generate light for illuminating the sample 900. The objective lens assembly 300 may be disposed between the light source assembly 200 and the image mirror assembly 100 so as to condense light generated by interaction with the sample 900 into parallel light or nearly parallel light. The imaging assembly 400 may be disposed on an outgoing light path of the imaging mirror assembly 100, so that the imaging mirror assembly 100 may collect parallel light or near-parallel light onto the imaging assembly 400. The imaging assembly 400 may include a Charge Coupled Device (CCD) or a complementary metal oxide semiconductor device (CMOS). The distance between the image mirror assembly 100 and the objective lens assembly 300 may be calculated primarily based on the Numerical Aperture (NA) of the objective lens assembly 300, the stop diameter of the exit pupil, the exit pupil angle, and the diameter of the image mirror assembly 100. In some embodiments, the distance between the imaging mirror assembly 100 and the objective lens assembly 300 may be 50-120 mm.

Furthermore, the present disclosure also proposes an optical detection apparatus, which may comprise an imaging mirror assembly or a microscopic device as described above. In the optical detection device, illumination light or excitation light generated by the light source assembly can be projected onto a sample at the sample stage to realize illumination of the sample so as to perform optical observation; or a signal such as fluorescence of the sample is excited, so that the relevant properties of the sample are analyzed based on the acquired signal. In addition, in some embodiments, the optical inspection device may further include a memory, processor, or the like communicatively coupled to the imaging assembly to automatically store and process image information formed by the imaging assembly to further facilitate inspection.

In all examples shown and discussed herein, any specific values should be construed as merely illustrative, and not a limitation. Thus, other examples of the exemplary embodiments may have different values.

The words "front," "back," "top," "bottom," "over," "under," and the like in the description and in the claims, if any, are used for descriptive purposes and not necessarily for describing permanent relative positions. It is to be understood that the terms so used are interchangeable under appropriate circumstances such that the embodiments of the disclosure described herein are, for example, capable of operation in other orientations than those illustrated or otherwise described herein.

As used herein, the word "exemplary" means "serving as an example, instance, or illustration," and not as a "model" to be replicated accurately. Any implementation described herein by way of example is not necessarily to be construed as preferred or advantageous over other implementations. Furthermore, this disclosure is not limited by any expressed or implied theory presented in the preceding technical field, background, brief summary or the detailed description.

As used herein, the term "substantially" is intended to encompass any minor variation due to design or manufacturing imperfections, tolerances of the device or element, environmental effects and/or other factors. The word "substantially" also allows for differences from perfect or ideal situations due to parasitics, noise, and other practical considerations that may be present in a practical implementation.

The foregoing description may indicate elements or nodes or features that are "connected" or "coupled" together. As used herein, unless expressly stated otherwise, "connected" means that one element/node/feature is directly connected (or in direct communication) electrically, mechanically, logically, or otherwise with another element/node/feature. Similarly, unless expressly stated otherwise, "coupled" means that one element/node/feature may be directly or indirectly joined to another element/node/feature in a mechanical, electrical, logical, or other manner to permit interactions, even though not directly connected. That is, "coupled" is intended to encompass both direct and indirect coupling of elements or other features, including connections utilizing one or more intermediate elements.

It will be further understood that the terms "comprises" and/or "comprising," when used herein, specify the presence of stated features, integers, steps, operations, elements, and/or components, but do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, and/or components, and/or groups thereof.

Those skilled in the art will recognize that the boundaries between the above described operations are merely illustrative. The operations may be combined into a single operation, the single operation may be distributed among additional operations, and the operations may be performed at least partially overlapping in time. Moreover, alternative embodiments may include multiple instances of a particular operation, and the order of operations may be altered in other various embodiments. However, other modifications, variations, and alternatives are also possible. The specification and drawings are, accordingly, to be regarded in an illustrative rather than a restrictive sense.

Although some specific embodiments of the present disclosure have been described in detail by way of example, it should be understood by those skilled in the art that the above examples are for illustration only and are not intended to limit the scope of the present disclosure. The embodiments disclosed herein may be combined in any desired manner without departing from the spirit and scope of the present disclosure. Those skilled in the art will also appreciate that various modifications might be made to the embodiments without departing from the scope and spirit of the present disclosure. The scope of the present disclosure is defined by the appended claims.

Claims

1. An imaging mirror assembly for a microscopy apparatus, the imaging mirror assembly configured to converge parallel imaging beams for imaging at an imaging location, the imaging mirror assembly comprising:

a first gaussian lens group, wherein the first gaussian lens group includes a first positive lens and a first negative lens group disposed adjacent to each other, the first negative lens group includes a third positive lens and a third negative lens disposed adjacent to each other, the third positive lens is disposed between the first positive lens and the third negative lens;

a second gaussian lens group, wherein the second gaussian lens group includes a second positive lens and a second negative lens group disposed adjacent to each other, the second negative lens group includes a fourth positive lens and a fourth negative lens disposed adjacent to each other, the fourth positive lens is disposed between the fourth negative lens and the second positive lens; and

a diaphragm provided between the first gaussian lens group and the second gaussian lens group, the first negative lens group being located between the first positive lens and the diaphragm, the second negative lens group being located between the diaphragm and the second positive lens, and the diaphragm being configured to pass light within a preset distance from an optical axis of the imaging lens assembly and block light outside the preset distance;

wherein the third positive lens and the third negative lens are made of transparent materials having different dispersion coefficients and substantially the same refractive index, and the fourth positive lens and the fourth negative lens are made of transparent materials having different dispersion coefficients and substantially the same refractive index, so that the phase difference is eliminated.

2. The imaging lens assembly of claim 1, wherein the stop is disposed at a central location between the first gaussian lens set and the second gaussian lens set.

3. The imaging lens assembly of claim 1, wherein the first gaussian lens set and the second gaussian lens set are symmetrically disposed about the stop.

4. The imaging lens assembly of claim 1, wherein a first focal length of the first positive lens is equal to a second focal length of the second positive lens.

5. The imaging lens assembly of claim 1, wherein a third focal length of the first negative lens group is equal to a fourth focal length of the second negative lens group.

6. The imaging lens assembly of claim 1, wherein a seventh focal length of the third positive lens is equal to an eighth focal length of the fourth positive lens.

7. The imaging lens assembly of claim 1, wherein a ninth focal length of the third negative lens is equal to a tenth focal length of the fourth negative lens.

8. The imaging mirror assembly of claim 1, wherein the focal length of the imaging mirror assembly is 50-80 mm.

9. A microscopy apparatus comprising an imaging mirror assembly according to any one of claims 1 to 8.

10. The microscopy device of claim 9, further comprising:

a light source assembly configured to generate light for illuminating a sample;

11. The microscopy device of claim 10, wherein the distance between the imaging mirror assembly and the objective lens assembly is 50-120 mm.

12. An optical detection apparatus, characterized in that it comprises an imaging mirror assembly according to any one of claims 1 to 8 or a microscopy device according to any one of claims 9 to 11.