CN114686353A - Detection module and detection device - Google Patents

Abstract

The invention discloses a detection module, which comprises a temperature control component and a power component for driving reaction liquid to move in a reagent card; the temperature control assembly comprises a temperature control seat, wherein a mounting groove for mounting the reagent card is formed in the temperature control seat, and a heating zone for keeping the temperature of the corresponding area of the reagent card at a set temperature is formed in the side wall of the mounting groove at intervals. The invention also provides a detection device which comprises a base substrate and an optical detection assembly, wherein the base substrate is provided with at least one detection module. The detection module and the detection device can effectively improve the temperature rise and fall rate of the reaction liquid, shorten the reaction time and improve the detection efficiency.

Description

Detection module and detection device

Technical Field

The invention belongs to the technical field of medical instruments, and particularly relates to a detection module and a detection device.

Background

Polymerase Chain Reaction (PCR) (polymerase Chain reaction) is short, PCR is a method for synthesizing specific DNA fragments in vitro enzymatically, the denaturation and renaturation of DNA are controlled by temperature change, a designed primer is added, DNA polymerase and dNTP can finish in vitro replication of specific genes, and periodic cycle treatment is carried out by heating at different temperatures and other steps at different stages, so that the target DNA can be rapidly amplified. The PCR has the characteristics of strong specificity, high sensitivity, simple and convenient operation, time saving and the like; it can be used not only for basic research of gene separation, cloning and nucleic acid sequence analysis, but also for diagnosis of diseases.

The PCR is composed of a plurality of basic reaction steps such as denaturation, annealing and extension, each step needs specific temperature control, and the current PCR instruments on the market mainly have the following problems:

1) the fluorescence quantitative PCR detection in the prior art comprises the steps of sample pretreatment, nucleic acid extraction, PCR reaction liquid preparation, loading and the like, the detection process needs to be finished in a strict PCR partition laboratory, professional operators and multiple manual pipetting operations are needed, the whole detection needs 3-4 hours to finish the report, and the time consumption is long; most of the fluorescent quantitative PCR instruments are high-flux large instruments, are expensive and are not suitable for primary hospitals or rapid field nucleic acid detection;

2) the temperature control system of the fluorescence quantitative PCR instrument in the prior art mostly adopts a semiconductor refrigerating sheet or an air bath, the reaction block repeatedly raises and lowers the temperature and then transfers the heat to a PCR reaction tube, the temperature raising and lowering speed of the reaction liquid is slow, dozens of amplification cycles need repeated temperature changing, and the time for raising and lowering the temperature in the middle is long, so that the whole amplification time is mostly 60-90 minutes, the time is long, and the time for completing the report of the whole detection is prolonged;

3) the PCR reaction system adopted by the fluorescent quantitative PCR instrument in the prior art is small (20-50ul), the repeated temperature rise and fall can continuously evaporate liquid in the tube into the tube cover to further influence the detection, so a hot cover with the temperature of 105 ℃ is added at the top of the PCR tube to prevent the evaporation of the liquid in the amplification process, the hot cover can integrally heat the PCR tube, and the risk of scalding hands when a user takes out the PCR tube after the detection is finished is avoided;

4) the optical structure of the fluorescence quantitative PCR instrument in the prior art mostly adopts a mode of leading out optical fibers and adding a filter wheel, the structure is large, the cost is high, the whole detection time is also prolonged because multicolor fluorescence polling excitation, receiving and high flux share one optical system, and the difference between channels possibly exists because the time difference exists at the fluorescence acquisition time of each sample channel.

Disclosure of Invention

In view of the above, the present invention provides a detection module and a detection apparatus, which can effectively increase the temperature increase and decrease rate of the reaction solution, shorten the reaction time, and increase the detection efficiency.

In order to achieve the purpose, the invention provides the following technical scheme:

the invention firstly provides a detection module, which comprises a temperature control component and a power component for driving reaction liquid to move in a reagent card;

the temperature control subassembly includes the control by temperature change seat, be equipped with the mount pad on the control by temperature change seat, be equipped with the mounting groove that is used for installing the reagent card in the mount pad, the interval is equipped with and is used for making in the mounting groove the temperature that the reagent card corresponds the region keeps in the zone of heating of settlement temperature.

Further, be equipped with in the mounting groove and be used for making the laminating pressure mechanism of reagent card and its one side lateral wall laminating, just the mounting groove with be equipped with on the lateral wall of reagent card laminating the zone of heating.

Further, the fitting pressure mechanism comprises a pressure mechanism for applying pressure to the reagent card; or the fit pressure mechanism comprises a tension mechanism for applying tension to the reagent card.

Further, the pressure mechanism comprises a plurality of pressure elastic elements.

Further, the number of the pressure elastic elements is at least three, and all the pressure elastic elements are not on the same straight line.

Furthermore, the pressure elastic element adopts a pressure spring or a pressure elastic sheet.

Furthermore, a temperature-changing transition mechanism for enabling the temperature of the corresponding area of the reagent card to be between the heating temperatures of the two heating areas is arranged between the two adjacent heating areas.

Further, the temperature-changing transition mechanism comprises a heat dissipation air duct arranged at the bottom or on the side wall of the mounting groove; or the temperature-changing transition mechanism comprises a transition heating zone arranged on the side wall of the mounting groove.

Further, the reagent card comprises a liquid position detection mechanism for detecting the position change of the reaction liquid in the flow channel of the reagent card.

Further, the liquid position detection mechanism comprises an LED transmitting end and a PD receiving end, and the LED transmitting end and the PD receiving end are respectively and correspondingly arranged on the side walls of the two sides of the mounting groove.

Further, the mounting seat comprises a first mounting seat (37) and a second mounting seat (38) which are arranged oppositely, a groove group is correspondingly arranged between the first mounting seat (37) and the second mounting seat (38) respectively, the groove group comprises a first groove arranged in the first mounting seat (37) and a second groove arranged in the second mounting seat (38), and the first groove and the second groove jointly form the mounting groove (33).

Further, the first mounting seat (37) and the second mounting seat (38) are made of heat conducting materials; the heating device is in one-to-one correspondence with the first installation seat (37) and the second installation seat (38), heats the corresponding first installation seat (37) or the second installation seat (38), and forms the heating area (34) in the installation groove (33).

Further, a top bead (39); the top bead (39) is arranged on the first mounting seat (37) and tightly presses the reagent card (32) on the second mounting seat (38), and/or the top bead (39) is arranged on the second mounting seat (38) and tightly presses the reagent card (32) on the first mounting seat (37).

Further, the power assembly comprises a piston rod and a movement driving mechanism for driving the piston rod to move along the axial direction of the piston rod; the piston rod is coaxial with the piston groove of the reagent card, and the front end of the piston rod is provided with a piston I matched with the piston groove of the reagent card; or the front end of the piston rod is connected with a piston II arranged in a piston groove of the reagent card.

Further, the front end of the piston rod (61) is detachably connected and matched with the piston II (18).

Furthermore, the moving driving mechanism comprises a sliding seat which is fixedly installed, a piston rod seat which is in sliding fit with the sliding seat and a power mechanism which is used for driving the piston rod seat to move along the axis of the piston rod seat are arranged on the sliding seat, and the piston rod is connected with the piston rod seat.

Furthermore, the power mechanism comprises a driving motor and a threaded screw rod in transmission connection with the driving motor, and the threaded screw rod is in threaded fit with the piston rod seat.

Furthermore, a position detection sensor for detecting the position of the piston rod seat is arranged on the sliding seat.

Furthermore, be equipped with at least one mounting groove that is used for installing the reagent card in the control by temperature change seat, the piston rod with the mounting groove one-to-one sets up, and all the piston rod all with the piston rod seat is connected.

Furthermore, the front end of the piston rod is detachably connected and matched with a piston II arranged in a piston groove of the reagent card; still include with the piston groove of reagent card corresponds the stopping hole that sets up, stopping hole with correspond the coaxial setting of piston groove, just the internal diameter more than or equal to in stopping hole the external diameter of piston rod is less than the external diameter of piston II.

The invention also provides a detection device which comprises a base substrate and an optical detection assembly, wherein the base substrate is provided with at least one detection module.

Further, the optical detection component comprises an optical module and an optical probe which is correspondingly arranged in the optical detection area of the reagent card.

Furthermore, the optical modules and the mounting grooves are arranged in a one-to-one correspondence manner; or, be equipped with on the base plate and detect the slide rail, optical module sliding fit installs detect on the slide rail, be equipped with on the base plate and be used for the drive optical module along detect the detection actuating mechanism that the slide rail removed.

Further, detect actuating mechanism including detect the motor and with detect the detection lead screw that the motor drive is connected, the last connection of optical module is equipped with the support, the support with detect lead screw thread fit.

Furthermore, a detection positioning assembly for positioning the optical module is further arranged on the base substrate.

Furthermore, the detection positioning assembly comprises a positioning plate parallel to the detection slide rail, positioning grooves or positioning holes are formed in the positioning plate and the mounting groove in a one-to-one correspondence mode, and a positioning sensor used for detecting the positions of the positioning grooves or the positioning holes is arranged on the support.

Further, still include the shell, base plate and all detection module all set up in the shell.

Furthermore, the shell is provided with a flip cover which can expose or close the corresponding detection module in one-to-one correspondence with each detection module.

The invention has the beneficial effects that:

according to the detection device, the mounting groove is formed in the temperature control seat and used for mounting the reagent card, the heating zone is arranged on the side wall of the mounting groove and used for directly heating the constant temperature zone of the reagent card, and when the reaction liquid flows into the corresponding constant temperature zone in the flowing process of the reaction liquid in the flow channel of the reagent card, the reaction liquid can be kept at the set temperature under the heating action of the heating zone, so that the reaction liquid can be quickly heated or cooled between different constant temperature zones to meet the reaction requirement, the heating and cooling rates of the reaction liquid can be effectively improved, the reaction time is shortened, and the reaction efficiency is improved.

The detection device of the invention also has the following technical effects:

1. the reagent card is arranged in the mounting groove, namely in the detection process, the mounting groove and the reagent card are relatively static, namely a movement gap is not required to be reserved between the reagent card and the mounting groove, so that the reagent card can be attached to the heating area, the heating area directly heats the corresponding constant-temperature area of the reagent card, the mode that air heat transfer is required in the prior art is abandoned, the heating is faster, and the heat transfer efficiency is higher;

2. by arranging the variable-temperature transition mechanism, on one hand, two adjacent heating zones can be isolated, so that the problem of unstable temperature caused by heat transfer between the two adjacent heating zones is solved, and on the other hand, the reaction liquid in the reagent card can be preheated or precooled, so that the reaction liquid can be heated or cooled to a set temperature more quickly after reaching the next constant-temperature zone;

3. the detection modules are mutually independent and support the simultaneous detection of a plurality of samples, so that the problem that only one sample can be detected at the same time by the conventional fluorescent quantitative PCR instrument is solved; meanwhile, the use requirement of plug and play can be met, namely, the samples can be detected by utilizing mutually independent detection modules at any time, waiting is not needed, and the method is applicable to on-site rapid nucleic acid detection.

Drawings

In order to make the object, technical scheme and beneficial effect of the invention more clear, the invention provides the following drawings for explanation:

FIG. 1 is a schematic diagram of a reagent card;

FIG. 2 is a schematic structural diagram of the reagent card after the sampling tube is inserted into the sampling tube placement slot and then pierced by the piercing needle;

FIG. 3 is a schematic diagram of another configuration of a reagent card;

FIG. 4 is a schematic structural diagram of an embodiment of the detecting device of the present invention;

FIG. 5 is a schematic structural diagram of a base substrate and a detection module mounted on the base bottom plate;

FIG. 6 is a schematic structural diagram of a temperature control assembly;

FIG. 7 is a front view of the temperature control assembly;

FIG. 8 is a schematic structural view of the bonding pressure mechanism;

FIG. 9 is a schematic view of the structure of the liquid position detection mechanism;

FIG. 10 is a schematic structural view of a power assembly;

FIG. 11 is a schematic structural view before the piston rod is connected with the piston II;

FIG. 12 is a schematic structural view when the piston rod is separated from the piston II;

fig. 13 is a schematic structural diagram of an optical detection assembly.

Description of reference numerals:

10-reagent card body; 11-a flow channel; 11 a-bending section; 11 b-a reaction section; 11 c-a rectifying section; 12-a sample entry zone; 13-a sampling tube; 14-a sampling tube placement slot; 15-puncture needle; 16-a piston groove; 17-the airway; 18-piston II; 18 a-an annular projection; 19-constant temperature area; 20-a temperature-changing transition zone; 21-heat dissipation holes; 22-a reactive agent; 23-sealing the plug; 24-an optical detection zone; 25-positioning holes.

30-a temperature control component; 31-a temperature control seat; 32-reagent card; 33-mounting grooves; 34-a heating zone; 35-a pressure elastic element; 36-a heat dissipation air duct; 37-a retaining wall; 38-a retaining wall; 39-top bead; 40-LED emitting end; 41-PD receiving end.

50-an optical detection assembly; 51-an optical module; 52-an optical probe;

60-a power assembly; 61-a piston rod; 62-a sliding seat; 63-piston rod seat; 64-a drive motor; 65-threaded lead screw; 66-a position detection sensor; 67-stop hole; 68-anti-regressive tablets; 69-a connector; 69 a-large diameter head section; 69 b-reducing head section; 81-an embedding cavity; 81 a-a large diameter cavity section; 81 b-a reduced diameter cavity section;

70-a detection module; 71-a base substrate; 72-detecting a slide rail; 73-detection motor; 74-detection screw rod; 75-a scaffold; 76-a positioning plate; 77-positioning groove; 78-a positioning sensor; 79-a housing; 80-a flip cover.

Detailed Description

The present invention is further described with reference to the following drawings and specific examples so that those skilled in the art can better understand the present invention and can practice the present invention, but the examples are not intended to limit the present invention.

Fig. 1 is a schematic diagram of a reagent card. The reagent card comprises a reagent card body 10, wherein a flow channel 11 for reaction liquid to flow through and a reaction liquid flow driving mechanism for driving the reaction liquid to flow in the flow channel 11 are arranged in the reagent card body 10. One end of the flow channel 11 is provided with a sample injection area 12 for injecting reaction liquid, and a sample injection mechanism is arranged in the sample injection area 12. Specifically, the sample injection mechanism includes a sampling tube placement groove 14 provided in the reagent card body 10 and used for inserting the sampling tube 13, and a liquid injection mechanism for injecting the reaction liquid into the flow channel 11 is provided in the sampling tube placement groove 14. The liquid injection mechanism comprises a puncture needle 15, the needle tip of the puncture needle 15 extends into the sampling tube placing groove 14 from the groove bottom of the sampling tube placing groove 14, and the needle tail of the puncture needle 15 is communicated with the flow channel 11. Preferably, the puncture needle 15 and the sampling tube placing groove 14 are coaxially arranged, the axis of the sampling tube placing groove 14 is located in the vertical direction, the upper end of the sampling tube placing groove 14 is opened, and the needle tip of the puncture needle 15 extends into the sampling tube placing groove 14 along the direction from the bottom to the top. When the device is used, the sampling tube 13 is inserted into the sampling tube placement groove 14, the sampling tube placement groove 14 plays a role in positioning the sampling tube 13, so that the axis of the sampling tube 13 is parallel to or coaxial with the axis of the sampling tube placement groove 14, and the puncture needle 15 can puncture the tube bottom of the sampling tube 13, so that the reaction liquid can be injected into the runner 11, as shown in fig. 2.

Specifically, the reaction liquid flow driving mechanism may be implemented in various conventional manners, as shown in fig. 2, the reaction liquid flow driving mechanism includes a piston groove 16, an air passage 17 communicated with the flow passage 11 is provided at a bottom of the piston groove 16, and a piston 18 and a piston driving mechanism for driving the piston 18 to move in the piston groove 16 are provided in the piston groove 16. The piston 18 is driven by the piston driving mechanism to move in the piston groove 16, so that the reaction liquid can be driven to move in the flow channel 11. By directly arranging the reaction liquid flow driving mechanism in the reagent card body 10, the problem of a power source of the existing microfluidic product is solved, the volume of the whole detection device can be reduced, and the detection device can be made smaller. Preferably, at least one narrow flow passage section 17a is provided in the air passage 17 for preventing the reaction liquid from passing through, and the narrow flow passage section 17a can prevent water vapor, liquid droplets and the like from passing through, thereby preventing the reaction liquid from entering the piston groove 16. The narrow flow path section 17a of the present embodiment is set to 2 sections, wherein one narrow flow path section 17a is directly connected to the flow path 11.

Further, at least two constant temperature zones 19 for maintaining the reaction solution in the flow channel 11 at a predetermined temperature are provided at intervals on the reagent card body 10. As shown in fig. 1, two constant temperature regions 19 are provided, and the temperatures of the two constant temperature regions 19 are not equal. Specifically, in the PCR reaction process, a constant temperature area 19 close to one side of the sample injection area 12 is set as a high temperature area, and the temperature of the constant temperature area is set to be 85-105 ℃; another thermostatic zone 19 is set as a low temperature zone, the temperature of which is set to 50-72 ℃. Of course, the number of the constant temperature areas 19 may also be set to three or more according to actual reaction requirements, as shown in fig. 3, the number of the constant temperature areas 19 is set to three, one constant temperature area 19 located in the middle is a low temperature area, and two constant temperature areas 19 located at two sides are high temperature areas. Specifically, the flow channel 11 sequentially passes through all the constant temperature regions 19, as shown in fig. 2, at this time, the reaction liquid can be driven by the reaction liquid flow driving mechanism to reciprocate in the flow channel 11, so as to achieve the technical purpose of circularly heating and cooling; or, at least two turn-back sections 11a are arranged on the flow channel 11, and of the two adjacent turn-back sections 11a, one of the turn-back sections 11a sequentially passes through all the constant temperature areas 19 along one direction, and the other turn-back section 11a sequentially passes through all the constant temperature areas 19 along the opposite direction, as shown in fig. 3, at this time, the reaction liquid flow driving mechanism can be used to drive the reaction liquid to flow in the flow channel 11 and sequentially enter each constant temperature area 19, so as to achieve the technical purpose of circularly increasing and decreasing the temperature.

Further, a variable temperature transition zone 20 for transitionally changing the temperature of the reaction liquid between the temperatures of the two constant temperature zones 19 is provided between the two adjacent constant temperature zones 19. Through setting up alternating temperature transition zone 20, can keep apart two adjacent constant temperature district 19 on the one hand to prevent to lead to the unstable problem of temperature between the adjacent constant temperature district 19 heat transfer, on the other hand can make and preheat or precool reaction liquid, can raise the temperature more fast or cool down to the settlement temperature after making reaction liquid reach next constant temperature district. As shown in fig. 1, a variable temperature transition zone 20 is disposed between the two constant temperature zones 19, and a plurality of variable temperature heat dissipation holes 21 are disposed in the variable temperature transition zone 20. Preferably, the flow channel 11 is bent in the constant temperature region 19 and the variable temperature transition region 20 in a serpentine shape, so that the length of the flow channel 11 in the corresponding constant temperature region 19 and variable temperature transition region 20 can be effectively increased, the flow channel 11 can be made thinner under the condition of accommodating reaction liquids with the same volume, the heating area and the heat utilization rate can be improved, and meanwhile, the areas of the constant temperature region 19 and the variable temperature transition region 20 can be made smaller, so that the volume of the equipment is reduced.

Further, a reaction section 11b is provided on the flow channel 11, and a reaction reagent 22 is stored in the reaction section 11 b. Specifically, the inner diameter of the reaction section 11b is larger than the inner diameter of other areas of the flow channel 11, and the liquid inlet end of the reaction section 11b is provided with the rectifying section 11c coaxial with the reaction section 11b, so that bubbles in the reaction liquid can float upwards more easily due to the fact that the inner diameter of the reaction section 11b is gradually increased from the rectifying section 11c upwards, and the generation of bubbles in the reaction liquid can be reduced. The reagent card body 10 is provided with a feed inlet which is communicated with the reaction section 11b and used for adding the reaction reagent 22 into the reaction section 11b, so that the reaction reagent 22 can be conveniently added, and the feed inlet is provided with a sealing plug 23. Specifically, the reaction reagent 22 is a plurality of lyophilized pellets stored in the reaction section 11 b. Preferably, the reaction section 11b is located in one of the constant temperature zones 19, and allows the reaction solution and the reaction reagent 22 to react in a set temperature environment. The reaction section 11b is arranged in the low-temperature region, and the other end of the reaction section 11b opposite to the liquid inlet end thereof is connected with the air flue 17.

Further, the reagent card body 10 is provided with an optical detection area 24 corresponding to the reaction section 11b, and detection light enters the flow channel 11 through the reagent card body 10, and liquid in the flow channel 11 is excited to generate fluorescence, so that optical detection is realized.

Further, the reagent card body 10 is further provided with at least two positioning holes 25, which is convenient for installing and positioning the reagent card.

The following is a detailed description of the specific embodiment of the detecting device of this embodiment.

Fig. 4 is a schematic structural diagram of an embodiment of the detecting device of the present invention. The detection device of the embodiment includes a base substrate 71 and an optical detection assembly 50, wherein at least one detection module 70 is disposed on the base substrate 71. The detection module of the present embodiment includes a temperature control assembly 30 and a power assembly 60 for driving the reaction solution to move in the reagent card 32. The temperature control assembly of the embodiment comprises a temperature control seat 31, wherein a mounting groove 33 for mounting a reagent card 32 is arranged in the temperature control seat 31, and a heating zone 34 for keeping the temperature of a corresponding area of the reagent card at a set temperature is arranged on the side wall of the mounting groove at intervals. Specifically, after the reagent card 32 is installed in the installation slot 33, the heating zones 34 of the present embodiment are disposed in one-to-one correspondence with the constant temperature zones 19 of the reagent card 32, and the temperature of each constant temperature zone 19 can be controlled to be at the set temperature by using the heating zones 34, so that the reaction solution in the flow channel 11 of the reagent card 31 can be controlled to be maintained at the set temperature in each constant temperature zone 19. The mounting groove 32 is provided in the temperature control socket 31. In the present embodiment, two temperature control units are provided in parallel on the temperature control holder 31, and two reagent cards 32 can be simultaneously mounted. The heating zone 34 of the present embodiment is made of a heat conductive material, and will not be described in detail.

Further, a fitting pressure mechanism for fitting the reagent card 32 to a side wall of one side thereof is provided in the mounting groove 33 of the present embodiment, and a heating area 34 is provided on a side wall of the mounting groove 33 to which the reagent card 32 is fitted. The bonding pressure mechanism comprises a pressure mechanism arranged to apply pressure to the reagent card 32; alternatively, the contact pressure mechanism includes a tension mechanism for applying a tension to the reagent card 32, and the tension mechanism may include at least three tension elastic elements that are not in the same straight line, and the application point of the tension elastic element on the reagent card 32 is located on the side of the reagent card 32 facing away from the heating region 34. The attaching pressure mechanism of the present embodiment employs a pressure mechanism for applying pressure to the reagent card 32, which is provided on the other side wall of the mounting groove 33. Specifically, the pressure mechanism includes a plurality of pressure elastic elements 35, the number of the pressure elastic elements 35 in this embodiment is at least three, all the pressure elastic elements 35 are not in the same straight line, and the three pressure elastic elements 35 which are not in the same straight line simultaneously act on one side surface of the reagent card 32, so that the reagent card can be attached to the heating region 34 more stably. The pressure elastic element 35 is a pressure spring or a pressure spring sheet, and the pressure elastic element 35 of the embodiment is a pressure spring sheet, so that the reagent card 32 can be conveniently inserted into the mounting groove 33 or pulled out from the mounting groove 33.

Furthermore, a temperature-changing transition mechanism for enabling the temperature of the corresponding area of the reagent card 32 to be between the heating temperatures of the two heating areas 34 is arranged between the two adjacent heating areas 34. Specifically, the temperature-changing transition mechanism is arranged corresponding to the temperature-changing transition area 20 of the reagent card 32. The temperature-changing transition mechanism can be realized in various ways, for example, the temperature-changing transition mechanism can include a heat dissipation air duct 36 disposed at the bottom or on the side wall of the installation groove 33; or, the temperature-changing transition mechanism may include a transition heating zone disposed on the side wall of the mounting groove 33, and the temperature of the reaction solution in the flow channel 11 of the reagent card 32 in the temperature-changing transition zone 20 is controlled by the transition heating zone to be located between the two constant temperature zones 19 on both sides thereof, so as to realize rapid temperature rise or temperature reduction of the reaction solution and improve efficiency. The temperature-varying transition mechanism of the present embodiment employs a heat dissipation air duct 36 disposed at the bottom or on the side wall of the mounting groove 33, and specifically, a plurality of temperature-varying heat dissipation holes 21 are disposed in the temperature-varying transition region 20 of the reagent card 32.

Further, the temperature control unit of the present embodiment further includes a liquid position detection mechanism for detecting a change in the position of the reaction liquid in the flow channel 11 of the reagent card 32. The liquid position detecting mechanism of the present embodiment includes an LED emitting end 40 and a PD receiving end 41, and the LED emitting end 40 and the PD receiving end 41 are respectively and correspondingly disposed on the two side walls of the mounting groove 33.

Further, the mounting seat of the present embodiment includes a first mounting seat 37 and a second mounting seat 38 that are disposed oppositely, a groove group is correspondingly disposed between the first mounting seat 37 and the second mounting seat 38, each groove group includes a first groove disposed in the first mounting seat 37 and a second groove disposed in the second mounting seat 38, and the first groove and the second groove belonging to the same groove group jointly form the mounting groove 33. Specifically, the first mounting seat 37 and the second mounting seat 38 of the present embodiment are made of a heat conductive material, and the first mounting seat 37 and the second mounting seat 38 of the present embodiment are made of metal aluminum. The temperature control unit of this embodiment further includes a heating device corresponding to the first mounting seat 37 and the second mounting seat 38, and the heating device heats the corresponding first mounting seat 37 or the second mounting seat 38 and forms the heating region 34 in the mounting groove 33. Specifically, the heating device may be directly disposed in the first and second mounting seats 37 and 38, or may be disposed outside the first and second mounting seats 37 and 38 and control the temperature of the first or second mounting seat 37 or 38, thereby controlling the temperature of each heating region 34. The temperature control cell of this embodiment also includes a top bead 39. A top bead 39 is disposed on the first mount 37 and secures the reagent card 32 against the second mount 38, and/or a top bead 39 is disposed on the second mount 38 and secures the reagent card 32 against the first mount 37. The top bead 39 of the present embodiment is disposed on the first mounting seat 37 to ensure that the mounting position of the reagent card 32 in the mounting groove 33 is accurate, so as to facilitate the smooth proceeding of the subsequent measurement operation.

Further, the power assembly 60 includes a piston rod 61 and a movement driving mechanism for driving the piston rod 61 to move in the axial direction thereof; the piston rod 61 is coaxial with the piston groove 16 of the reagent card 32, and the front end of the piston rod 61 is provided with a piston I matched with the piston groove 16 of the reagent card 32; or the front end of the piston rod 61 is connected with the piston II 18 arranged in the piston groove 16 of the reagent card 32. The front end of the piston rod 61 of the embodiment is detachably connected and matched with the piston II 18 arranged in the piston groove 16 of the reagent card 32. The front end of the piston rod 61 of the embodiment is provided with a connector 69, the rear end face of the piston II 18 is provided with an embedding cavity 81 matched with the connector 69, and the piston rod 61 and the piston II 18 are detachably connected and matched through the connector 69 and the embedding cavity 81. The power assembly of the embodiment further comprises a retaining hole 67 which is arranged corresponding to the piston groove 16 of the reagent card 32, the retaining hole 67 is arranged coaxially with the corresponding piston groove 16, and the inner diameter of the retaining hole 67 is larger than or equal to the outer diameter of the piston rod 61 and smaller than the outer diameter of the piston II 18. Specifically, the notch of the piston groove 16 of the present embodiment is provided with a retaining piece 68, and the retaining hole 67 is provided in the retaining piece 68. Through setting up stopping hole 67, when piston rod 61 withdraws from piston groove 16, under the effect of stopping hole 67, piston rod 61 separates with piston II 18, makes piston II 18 stay in piston groove 16, plays the effect of sealed piston groove 16, prevents that reaction solution from revealing and polluting detection device.

Further, the connector 69 includes a large-diameter head section 69a located at the front end and a reducing head section 69b located at the rear side of the large-diameter head section 69a, and the outer diameter of the reducing head section 69b is smaller than that of the large-diameter head section 69 a; the insertion cavity 81 includes a large-diameter cavity section 81a fitted with the large-diameter head section 69a and a reduced-diameter cavity section 81b fitted with the reduced-diameter head section 69b, the inner diameter of the reduced-diameter cavity section 81b is smaller than the inner diameter of the large-diameter cavity section 81a and the outer diameter of the large-diameter head section 69a, respectively, and the large-diameter head section 69a and the large-diameter cavity section 81a are in interference fit. After the connector 69 is inserted into the insertion cavity 81, the piston II 18 and the piston rod 61 can synchronously move in the piston groove 16, and the piston II 18 and the piston rod 61 can be prevented from being separated under the action of small external force. Preferably, the outer diameter of the front end surface of the large-diameter head section 69a gradually increases in the front-to-rear direction, and serves as a guide for expanding and contracting the cavity section 81 b. The front end surface of the large-diameter head section 69a in the present embodiment is a spherical surface, but of course, the front end surface of the large-diameter head section 69a may also be a conical surface, which will not be described in detail.

Further, the movement driving mechanism comprises a sliding seat 62 fixedly installed, a piston rod seat 63 in sliding fit with the sliding seat 62 and a power mechanism for driving the piston rod seat 63 to move along the axis of the piston rod seat 63 are arranged on the sliding seat 62, and the piston rod 61 is connected with the piston rod seat 63. The power mechanism of the embodiment comprises a driving motor 64 and a threaded screw rod 65 in transmission connection with the driving motor 64, wherein the threaded screw rod 65 is in threaded fit with the piston rod seat 63. The slide holder 62 of the present embodiment is provided with a position detection sensor 66 for detecting the position of the piston rod holder 63. Specifically, the position detection sensors 66 of the present embodiment are provided in two, and the two position detection sensors 66 are respectively located at the stroke initial position and the stroke end position of the piston rod base 63, and are used for controlling the stroke range of the piston rod 61 during the reciprocating movement in the piston groove 16. The position detection sensor 66 of the present embodiment employs a photoelectric switch.

Further, be equipped with at least one mounting groove 33 that is used for installing reagent card 32 in the temperature control seat 31, piston rod 61 and mounting groove 33 one-to-one set up, and all piston rods 61 all are connected with piston rod seat 63. The temperature control seat 31 of the present embodiment is provided with 2 mounting grooves 33 for mounting the reagent card 32 therein, i.e. the number of the piston rods 61 fixedly connected to the piston rod seat 63 is two.

Further, the piston ii 18 of the present embodiment is preset at the bottom of the piston groove 16, and when the piston rod 61 extends into the piston groove 16 and is connected to the piston ii 18, it is necessary to apply an axial pressure action to the piston ii 18, and under the action of the pressure, if the piston ii 18 is not located at the bottom of the piston groove 16, the piston ii 18 will also move to the bottom of the piston groove 16, so that the fluid position in other flow passages connected to the piston groove 16 will be changed, and it is not convenient for accurate control.

Furthermore, the outer peripheral wall of the piston II 18 is provided with at least one annular protrusion 18a which is in interference fit with the piston groove 16, and the annular protrusions 18a of the embodiment are arranged at intervals of 3, so that the sealing contact performance between the piston II 18 and the piston groove 16 is ensured.

Further, the optical detection assembly 50 includes an optical module 51 and an optical probe 52 disposed in correspondence with the optical detection zone 24 of the reagent card 32. The optical modules 51 may be disposed in one-to-one correspondence with the mounting grooves 33; the base substrate 71 of this embodiment is provided with a detection slide rail 72, the optical module 51 is installed on the detection slide rail 72 in a sliding fit manner, the base substrate 71 is provided with a detection driving mechanism for driving the optical module 51 to move along the detection slide rail 72, that is, the optical module 51 is installed on the detection slide rail 72 in a sliding fit manner, and the same optical module 51 can meet the detection requirements of a plurality of detection modules and a plurality of reagent cards 32. The detection driving mechanism of the embodiment comprises a detection motor 73 and a detection screw rod 74 in transmission connection with the detection motor 73, a bracket 75 is connected to the optical module 51, and the bracket 75 is in threaded fit with the detection screw rod 74.

Further, a detection positioning component for positioning the optical module 51 is disposed on the base substrate 71. The detecting and positioning assembly of the present embodiment includes a positioning plate 76 parallel to the detecting slide rail 72, the positioning plate 76 is provided with a positioning slot 77 or a positioning hole in one-to-one correspondence with the mounting groove 33, and the bracket 75 is provided with a positioning sensor 78 for detecting the position of the positioning slot 77 or the positioning hole. The position sensor 78 of the present embodiment employs a photoelectric switch.

Further, the detection apparatus of the present embodiment further includes a housing 79, and the base substrate 71 and all the detection modules 70 are disposed in the housing 79. The housing 79 of the present embodiment is provided with a flip 80 corresponding to each detection module 70, so that the corresponding detection module 70 can be exposed or closed. The number of the flip covers 80 is 4 in the present embodiment, and one detection module 70 is provided in each flip cover 80.

The above-mentioned embodiments are merely preferred embodiments for fully illustrating the present invention, and the scope of the present invention is not limited thereto. The equivalent substitution or change made by the technical personnel in the technical field on the basis of the invention is all within the protection scope of the invention. The protection scope of the invention is subject to the claims.

Claims (24)

1. A detection module, characterized by: comprises a temperature control component (30) and a power component (60) for driving reaction liquid to move in a reagent card (32);

the temperature control assembly comprises a temperature control seat (31), a mounting seat is arranged on the temperature control seat (31), a mounting groove (33) for mounting a reagent card (32) is formed in the mounting seat, and heating zones (34) for keeping the temperature of a corresponding area of the reagent card (32) at a set temperature are arranged in the mounting groove (33) at intervals; the heating zone (34) is made of heat conducting materials;

the power assembly (60) comprises a piston rod (61) and a movement driving mechanism for driving the piston rod (61) to move along the axial direction thereof.

2. The detection module of claim 1, wherein: a bonding pressure mechanism used for bonding the reagent card (32) with the side wall of one side of the reagent card is arranged in the mounting groove (33); the mounting groove (33) and the side wall attached to the reagent card (32) are provided with the heating zone (34).

3. The detection module of claim 2, wherein: the fitting pressure mechanism comprises a pressure mechanism for applying pressure to the reagent card (32); or, the fitting pressure mechanism comprises a tension mechanism for applying tension to the reagent card (32).

4. The detection module of claim 3, wherein: the pressure mechanism comprises a plurality of pressure elastic elements (35).

5. The detection module of claim 4, wherein: the number of the pressure elastic elements (35) is at least three, and all the pressure elastic elements (35) are not on the same straight line.

6. The detection module according to any one of claims 1 to 5, characterized in that: a temperature-changing transition mechanism for enabling the temperature of the corresponding area of the reagent card (32) to be between the heating temperatures of the two heating areas (32) is arranged between the two adjacent heating areas (34).

7. The detection module of claim 6, wherein: the temperature-changing transition mechanism comprises a heat dissipation air duct (36) arranged at the bottom or on the side wall of the mounting groove (33); or the temperature-changing transition mechanism comprises a transition heating zone arranged on the side wall of the mounting groove (33).

8. The detection module according to any one of claims 1 to 5, characterized in that: and a liquid position detection mechanism for detecting the position change of the reaction liquid in the flow channel (11) of the reagent card (32).

9. The detection module of claim 8, wherein: the liquid position detection mechanism comprises an LED emitting end and a PD receiving end, and the LED emitting end (40) and the PD receiving end (41) are respectively and correspondingly arranged on the side walls of the two sides of the mounting groove (33).

10. The detection module according to any one of claims 1 to 5, characterized in that: the mounting seat comprises a first mounting seat (37) and a second mounting seat (38) which are arranged oppositely, a groove group is correspondingly arranged between the first mounting seat (37) and the second mounting seat (38) respectively, the groove group comprises a first groove arranged in the first mounting seat (37) and a second groove arranged in the second mounting seat (38), and the first groove and the second groove jointly form the mounting groove (33).

11. The detection module of claim 10, wherein: the first mounting seat (37) and the second mounting seat (38) are made of heat conducting materials; the heating device is in one-to-one correspondence with the first installation seat (37) and the second installation seat (38), heats the corresponding first installation seat (37) or the second installation seat (38), and forms the heating area (34) in the installation groove (33).

12. The detection module of claim 10, wherein: also comprises a top bead (39); the top bead (39) is arranged on the first mounting seat (37) and tightly presses the reagent card (32) on the second mounting seat (38), and/or the top bead (39) is arranged on the second mounting seat (38) and tightly presses the reagent card (32) on the first mounting seat (37).

13. The detection module of claim 1, wherein: the piston rod (61) is coaxial with the piston groove (16) of the reagent card (32), and the front end of the piston rod (61) is provided with a piston I matched with the piston groove (16) of the reagent card (32); or the front end of the piston rod (61) is connected with a piston II (18) arranged in a piston groove (16) of the reagent card (32).

14. The detection module of claim 13, wherein: the front end of the piston rod (61) is detachably connected and matched with the piston II (18).

15. The detection module of claim 13, wherein: the movable driving mechanism comprises a sliding seat (62) fixedly installed, a piston rod seat (63) in sliding fit with the sliding seat (62) and a power mechanism used for driving the piston rod seat (63) to move along the axis of the piston rod seat, and the piston rod (61) is connected with the piston rod seat (63).

16. The detection module of claim 15, wherein: the power mechanism comprises a driving motor (64) and a threaded screw rod (65) in transmission connection with the driving motor (64), and the threaded screw rod (65) is in threaded fit with the piston rod seat (63).

17. The detection module of claim 16, wherein: and a position detection sensor (66) for detecting the position of the piston rod seat (63) is arranged on the sliding seat (62).

18. The detection module of claim 15, wherein: the mounting seat is internally provided with at least one mounting groove (33) for mounting a reagent card (32), the piston rods (61) are arranged in one-to-one correspondence with the mounting grooves (33), and all the piston rods (61) are connected with the piston rod seats (63).

19. The detection module according to any one of claims 13-18, wherein: the front end of the piston rod (61) is detachably connected and matched with a piston II (18) arranged in a piston groove (16) of the reagent card (32); still include with piston groove (16) of reagent card (32) correspond backstop hole (67) that sets up, backstop hole (67) with correspond piston groove (16) coaxial arrangement, just the internal diameter more than or equal to of backstop hole (67) the external diameter of piston rod (61) is less than the external diameter of piston II (18).

20. A detection device, characterized by: comprising a base substrate (71) and an optical detection assembly (50), said base substrate (71) being provided with at least one detection module (70) according to any one of claims 1-19.

21. The detection device according to claim 20, wherein: the optical detection assembly (50) comprises an optical module (51) and an optical probe (52) which is arranged corresponding to the optical detection area (24) of the reagent card (32).

22. The detection device according to claim 21, wherein: the optical modules (51) are arranged in one-to-one correspondence with the mounting grooves (33); or, a detection slide rail (72) is arranged on the base substrate (71), the optical module (51) is installed on the detection slide rail (72) in a sliding fit manner, and a detection driving mechanism for driving the optical module (51) to move along the detection slide rail (72) is arranged on the base substrate (71); the detection driving mechanism comprises a detection motor (73) and a detection screw rod (74) in transmission connection with the detection motor (73), a support (75) is connected to the optical module (51), and the support (75) is in threaded fit with the detection screw rod (74).

23. The detection device according to claim 22, wherein: the base substrate (71) is also provided with a detection positioning component for positioning the optical module (51); the detection positioning assembly comprises a positioning plate (76) parallel to the detection sliding rail (72), positioning grooves (77) or positioning holes are formed in the positioning plate (76) and the mounting groove (33) in a one-to-one correspondence mode, and a positioning sensor (78) used for detecting the positions of the positioning grooves (77) or the positioning holes is arranged on the support (75).

24. The detection device according to any one of claims 20 to 23, wherein: the base plate (71) and all the detection modules (70) are arranged in the shell (79); the shell (79) is provided with a flip (80) which is in one-to-one correspondence with each detection module (70) and can enable the corresponding detection module (70) to be exposed or closed.

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