CN212270116U

CN212270116U - Molecular detection system

Info

Publication number: CN212270116U
Application number: CN201921681143.8U
Authority: CN
Inventors: 胡军荣; 徐强; 崔相民
Original assignee: Shenyi Biotech Hangzhou Co Ltd
Current assignee: Shenyi Biotech Hangzhou Co Ltd
Priority date: 2019-07-01
Filing date: 2019-10-09
Publication date: 2021-01-01
Anticipated expiration: 2029-10-09
Also published as: CN112175817A

Abstract

The utility model discloses a molecular detection system. The molecular detection system includes: the device comprises a base body, wherein a fixing device, a driving device, a detection device and a temperature control device are arranged on the base body; wherein the fixing device is adapted to fix a container containing a sample to be tested; the driving device is suitable for driving the container to pretreat the sample to be detected; the detection device is suitable for detecting the preprocessed sample to be detected; the temperature control device is suitable for regulating and controlling the temperature of the sample to be detected. The molecular detection system can perform full-automatic rapid detection on a sample to be detected.

Description

Molecular detection system

Technical Field

The utility model relates to a molecular biology field, particularly, the utility model relates to a molecular detection system.

Background

Biomolecules and other analytes can be detected by selective or specific probes that react with them. With the development of molecular biology, the detection of biomolecules such as proteins, DNA, and RNA has become an effective means for the diagnosis and prediction of diseases. The advantages of molecular diagnosis technology, such as less sample consumption, accurate diagnosis result, high sensitivity, high flux and the like, enable the technology to develop rapidly in modern medicine.

Currently, the most widely used detection method in molecular diagnostic technology is fluorescence labeling detection technology. The technology takes a fluorescent molecular group as a marker, and connects the fluorescent marker to a probe molecule or a target molecule through a chemical modification way. The detection instrument irradiates laser with specific wavelength on a sample to be detected, and quantitative detection of specific molecules can be realized by detecting the intensity of corresponding fluorescent signals. However, the existing molecular detection means still need to be improved.

SUMMERY OF THE UTILITY MODEL

The present invention is proposed based on the discovery by the inventors of the following facts and problems:

the existing molecular detection instrument generally does not directly detect a sample, but needs a great deal of auxiliary work before detection. For example, according to the specific situation of the sample, the nucleic acid in the sample needs to be purified, the desired molecular fragment is extracted, the corresponding detection reagent is prepared, and finally the sample and the detection reagent are mixed and placed in the detection instrument for detection. The auxiliary work operation is complicated and needs to be completed through different instruments or tools, so that the molecular detection must be performed by professional personnel in a standard laboratory environment, the application scene is limited, the detection time is long, and the efficiency is low.

In view of this, the utility model provides a molecular detection system. The molecular detection system can perform full-automatic rapid detection on a sample to be detected.

In one aspect of the present invention, the utility model provides a molecular detection system. According to the utility model discloses an embodiment, this molecule detecting system includes: the device comprises a base body, wherein a fixing device, a driving device, a detection device and a temperature control device are arranged on the base body; wherein the fixing device is adapted to fix a container containing a sample to be tested; the driving device is suitable for driving the container to pretreat the sample to be detected; the detection device is suitable for detecting the preprocessed sample to be detected; the temperature control device is suitable for regulating and controlling the temperature of the sample to be detected.

According to the utility model discloses molecular detection system, on will containing the container that detects the sample and be fixed in fixing device, under drive arrangement's effect, detect the sample and accomplish extraction and the PCR reaction of nucleic acid in the sample when flowing in the container to the acquisition is suitable for detection device to carry out the sample that detects. In the process that the driving device drives the sample to be detected to flow in the container, the temperature control device can heat the local part in the fixing device, so that the extraction of nucleic acid in the sample and the PCR reaction are realized. While the PCR reaction is carried out, the detection device can carry out real-time fluorescence measurement on the amplified product, and a detection result is obtained according to the data of the change of the fluorescence signal. Therefore, the utility model discloses a molecular detection system integrates the sample nucleic acid's supplementary work such as purification, extraction in an equipment to realized that molecular detection's full automatization is accomplished, solved traditional molecular detection means and restricted and applied the limited, inefficiency scheduling problem of scene by experimental condition and staff.

Optionally, the base includes a base, a support, and a sleeve coupled thereto.

Optionally, the fixing device comprises a clamping groove, a first motor, a first nut, a first clamping member, a second clamping member and a connecting rod; the first motor is connected with the first clamping component through the first nut, the first clamping component is connected with the second clamping component through the connecting rod, and the clamping groove is formed between the first clamping component and the second clamping component.

Optionally, the molecular detection system further comprises: and the pressure spring is arranged between the clamping groove and the second clamping component.

Optionally, the molecular detection system further comprises: a first heat dissipation assembly; the first heat dissipation assembly is arranged below the clamping groove.

Optionally, the driving device comprises a second motor, a third motor, a thimble and a push rod; the second motor is connected with the ejector pin, and the third motor is connected with the push rod.

Optionally, the molecular detection system further comprises: the push rod is arranged on the linear guide rail through the sliding block.

Optionally, the detection device includes a light path detection unit, and the temperature control device includes a chip temperature cycle control.

Optionally, one end of the detection device is connected to the card slot through the chip temperature circulation control.

Optionally, the molecular detection system further comprises: and the control system is respectively connected with the fixing device, the driving device, the detection device and the temperature control device and is suitable for controlling the fixing device, the driving device, the detection device and the temperature control device to work cooperatively so as to detect the sample to be detected.

Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.

Drawings

The above and/or additional aspects and advantages of the present invention will become apparent and readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings of which:

FIG. 1 is a schematic diagram of a molecular detection system according to one embodiment of the present invention;

FIG. 2 is a schematic diagram of a molecular detection system according to yet another embodiment of the present invention;

FIG. 3 is a schematic view of another perspective of a molecular detection system according to another embodiment of the present invention;

FIG. 4 is a schematic view of another perspective of a molecular detection system according to another embodiment of the present invention;

FIG. 5 is a schematic view of another perspective of a molecular detection system according to another embodiment of the present invention;

FIG. 6 is a schematic structural diagram of a middle subassembly of a fixing device, a driving device and a temperature control device in a molecular detection system according to an embodiment of the present invention;

FIG. 7 is a schematic structural diagram of a middle subassembly of a detection device in a molecular detection system according to an embodiment of the present invention;

FIG. 8 is a schematic view of a middle subassembly of a detection device in a molecular detection system according to an embodiment of the present invention;

FIG. 9 is a schematic structural view of a central subassembly of the fixture and the actuator in the molecular detection system according to an embodiment of the present invention;

FIG. 10 is a schematic view of an alternative perspective structure of the central subassembly of the fixture and the actuator in the molecular detection system of an embodiment of the present invention;

FIG. 11 is a schematic view of another perspective structure of a central subassembly of a fixture and a driver in a molecular detection system according to an embodiment of the present invention;

fig. 12 is a schematic structural diagram of a microfluidic cartridge according to an embodiment of the present invention.

Reference numerals:

100: a substrate; 200: a fixing device; 300: a drive device; 400: a detection device; 500: a temperature control device;

1: a base; 2: a support; 3: a shaft sleeve; 4: a first motor; 5: a first nut;

6: a card slot; 61: a first heat dissipation assembly; 6-1: a sample chamber; 6-2: a dilution chamber; 6-3: an injection chamber; 6-4: a piston; 6-5: a PCR chamber; 6-6: a buffer chamber; 6-7: a sample sealing film; 6-8: diluting the sealing film; 6-9: a microfluidic circuit; 6-10: a sample control valve; 6-11: a dilution control valve; 6-12: a first PCR control valve; 6-13: a second PCR control valve;

7: a temperature cycling control;

8: a clamping device; 81: a first clamping member; 82: a second clamping member; 83: a connecting rod;

9: a screw; 10: a pressure spring; 11: a second motor; 12: a thimble; 13: a third motor; 14: a motor bracket;

15: a second nut; 16: a push rod; 17: a linear guide rail; 18: a slider; 19: an optical path detection unit;

20: and (5) controlling the system.

Detailed Description

Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are exemplary and intended to be used for explaining the present invention, and should not be construed as limiting the present invention.

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", and the like, indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.

In the present invention, unless otherwise expressly specified or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly, e.g., as meaning permanently connected, detachably connected, or integral to one another; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.

In the present application, unless expressly stated or limited otherwise, the first feature may be directly on or directly under the second feature or indirectly via intermediate members. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature.

The inventor finds that the existing molecular detection instrument generally does not directly detect a sample, but needs a great deal of auxiliary work before detection in the research of the molecular detection instrument. For example, according to the specific situation of the sample, the nucleic acid in the sample needs to be purified, the desired molecular fragment is extracted, the corresponding detection reagent is prepared, and finally the sample and the detection reagent are mixed and placed in the detection instrument for detection. The auxiliary work operation is complicated and needs to be completed through different instruments or tools, so that the molecular detection must be performed by professional personnel in a standard laboratory environment, the application scene is limited, the detection time is long, and the efficiency is low.

In view of this, in one aspect of the present invention, the present invention provides a molecular detection system. According to an embodiment of the present invention, referring to fig. 1, the molecular detection system includes: a base body 100, and a fixing device 200, a driving device 300, a detecting device 400, and a temperature control device 500 provided on the base body 100. Wherein the holding device 200 is adapted to hold a container containing a sample to be tested; the driving device 300 is adapted to drive the container to pre-process the sample to be tested; the detection device 400 is suitable for detecting the pretreated sample to be detected, and the temperature control device 500 is suitable for regulating and controlling the temperature of the sample to be detected.

The molecular detection system of the present invention is further described in detail with reference to FIGS. 1 to 12. FIGS. 2 to 5 are schematic structural views of the molecular detection system at different viewing angles. Fig. 6 shows the relative positional relationship of the central sub-assemblies of the fixing means 200, the driving means 300 and the temperature control means 500 in detail. Fig. 7 and 8 show the relative positions of the subassemblies in the middle of the inspection device 400 in detail from different perspectives, respectively. FIGS. 9 to 11 show in detail the relative positions of the subassemblies in the center of the fixture 200 and the drive device 300, respectively, from different perspectives. Fig. 12 is a schematic view of the structure of a container containing a sample to be tested.

The specific structure of the base 100 is not particularly limited as long as it can provide support for the fixing device 200, the driving device 300, the detecting device 400 and the temperature control device 500, so that each device can stably operate. According to an embodiment of the present invention, referring to fig. 2 to 6, the base body 100 includes a base 1, a bracket 2, and a shaft sleeve 3 connected to each other. The base 1, the support 2 and the shaft sleeve 3 together form a support structure of the molecular detection system, and provide support for the fixing device 200, the driving device 300, the detection device 400 and the temperature control device 500.

The fixing device 200 is suitable for fixing a container containing a sample to be detected, and can ensure that the position of the container containing the sample to be detected is relatively fixed in the detection process, so that the pretreatment and the detection process of the sample to be detected are stably carried out. According to an embodiment of the present invention, referring to fig. 2 to 6, the fixing device comprises a clamping groove 6, a first motor 4, a first nut 5 and a clamping device 8, wherein the clamping device 8 comprises a first clamping member 81, a second clamping member 82 and a connecting rod 83; the first motor 4 is connected to the first clamping member 81 through the first nut 5, the first clamping member 81 is connected to the second clamping member 82 through the connecting rod 83, and the engaging groove 6 is provided between the first clamping member 81 and the second clamping member 82. As shown in fig. 2 to 6, the engaging groove 6 is provided on the base 1, the first motor 4 is provided on the bracket 2, and the connecting rod 83 of the clamping device 8 passes through the shaft sleeve 3 to connect the first clamping member 81 and the second clamping member 82. In the detection process, a container containing a sample to be detected is placed in the clamping groove 6, and along with the movement of the first nut 5 driven by the first motor 4, the first clamping member 81 and the second clamping member 82 in the clamping device 8 can clamp the container in the clamping groove 6, so that the position of the container is ensured to be relatively fixed in the detection process.

According to the utility model discloses an embodiment, this molecule detecting system can still further include: a compression spring 10. The compression spring 10 is arranged between the catch 6 and the second clamping member 82 and is loosely connected by means of a screw 9. Thus, during the movement of the holding device 8, the container in the locking groove 6 is clamped by the action of the compression spring 10.

According to the utility model discloses an embodiment, this molecule detecting system can still further include: a first heat dissipation assembly 61. The first heat sink 61 is disposed below the card slot 6. Specifically, the first heat dissipation assembly 61 may be mounted on the base 1 and disposed below the card slot 6. Through set up first radiator unit 61 in the below of draw-in groove 6, can be more favorable to containing the heat dissipation of the container that waits to detect the sample to be convenient for temperature control device regulation and control container temperature. The specific kind of the first heat dissipation assembly 61 is not particularly limited, and for example, a fin type heat sink, a plate type heat sink, or the like may be employed.

The driving device 300 is adapted to drive the container containing the sample to be tested to pre-process the second sample to be tested. The pretreatment comprises the extraction of nucleic acid in a sample to be detected and PCR reaction. For the convenience of understanding, the structure and the operation principle of the container containing the sample to be measured will be described in detail first. It should be noted that, herein, the "container containing the sample to be tested" is also referred to as "kit" or "microfluidic kit".

Referring to fig. 12, the kit comprises a sample chamber 6-1, a dilution chamber 6-2, an injection chamber 6-3, a piston 6-4, a PCR chamber 6-5, a buffer chamber 6-6, a sample sealing film 6-7, a dilution sealing film 6-8, a microfluidic pipeline 6-9, a sample control valve 6-10, a dilution control valve 6-11, a first PCR control valve 6-12 and a second PCR control valve 6-13, wherein the units are connected through the microfluidic pipeline 6-9 to form a communicated loop.

In the initial state, the sample chamber 6-1 contains lysis material in the form of lyophilized powder, the PCR chamber 6-5 contains reverse transcriptase and PCR material in the form of lyophilized powder, and the dilution chamber 6-2 contains appropriate diluent. The parts of the sample chamber 6-1 and the dilution chamber 6-2 communicated with the microfluidic pipeline 6-9 are respectively sealed by a sample sealing film 6-7 and a dilution sealing film 6-8, so that the cracking raw material of the sample chamber 6-1 is isolated from the diluent of the dilution chamber 6-2 and the reverse transcriptase and PCR raw material of the PCR chamber 6-5. The piston 6-4 is at the very top of the injection chamber 6-3 (the injection chamber is in a piston filled state).

When the sample is added to the sample chamber 6-1, the microfluidic cartridge starts to operate. First, the sample sealing film 6-7 and the diluting sealing film 6-8 are pierced, thereby allowing communication between the chambers and the micro-flow hole line. Secondly, the sample control valve 6-10, the first PCR control valve 6-12 and the second PCR control valve 6-13 are closed, and the movable piston 6-4 is pulled outwards to a certain position, so that the diluent in the diluent chamber 6-2 flows to the injection chamber 6-3 through the diluent control valve 6-11. And thirdly, closing the dilution control valve 6-11, opening the sample control valve 6-10, and moving the piston 6-4 in a reciprocating manner to enable the diluent in the injection chamber 6-3 to enter the sample chamber 6-1 through the sample control valve 6-10, and fully and uniformly mixing the lysis freeze-dried powder in the sample chamber 6-1 with the diluent and the added sample in the process of moving the piston 6-4 in a reciprocating manner. Fourth, the sample chamber 6-1 is heated to the set temperature, so that the sample in the sample chamber 6-1 is sufficiently lysed at the set temperature. Fifth, after the lysis is completed, the movable piston 6-4 is pulled outward to a position so that the lysed sample mixture in the sample chamber 6-1 flows to the injection chamber 6-3 through the sample control valve 6-10. And sixthly, closing the sample control valve 6-10 and the dilution control valve 6-11, opening the first PCR control valve 6-12 and the second PCR control valve 6-13, reciprocating the piston 6-4, enabling the sample mixed solution in the injection chamber 6-3 to enter the PCR chamber 6-5 through the first PCR control valve 6-12, and fully and uniformly mixing the cracked sample mixed solution with the reverse transcriptase and the PCR raw material freeze-dried powder in the PCR chamber 6-5 in the process of reciprocating the piston 6-4. And ninthly, starting to perform PCR temperature heating control on the PCR chamber 6-5, activating a previous constant temperature section of enzyme in PCR amplification, enabling the mixed solution in the PCR chamber 6-5 to expand due to high temperature, enabling the overflow of the liquid in the expansion process to flow into the buffer chamber 6-6 through the second PCR control valve 6-13, closing the first PCR control valve 6-12 and the second PCR control valve 6-13 after the constant temperature section is finished, starting to perform temperature cycle control on the PCR chamber 6-5, and finally completing the amplification of the nucleic acid in the sample.

According to an embodiment of the present invention, referring to fig. 9 to 11, the driving device 300 includes a second motor 11, a third motor 13, a thimble 12 and a push rod 16; the second motor 11 is a linear motor, the third motor 13 is a screw motor, the second motor 11 is directly connected with the thimble 12, and the third motor 13 is connected with the push rod 16 through a second nut 15. The second motor 11 and the thimble 12 may be directly mounted on the base 1, and the second motor 13 may be mounted on the base 1 through a motor bracket 14. The reagent kit containing the sample to be detected is fixed in the clamping groove 6, the positions of the sample sealing films 6-7 and the positions of the dilution sealing films 6-8 in the reagent kit are respectively opposite to one ejector pin 12, and the ejector pins 12 are driven by the second motor 11 to do linear motion, so that the sample sealing films 6-7 and the dilution sealing films 6-8 can be punctured, and the microfluidic pipelines in the reagent kit are communicated. And the third motor 13 can drive the push rod 16 to move linearly through the second nut 15 to control the reciprocating motion of the piston 6-4 in the reagent box, so that the sample in the reagent box flows in the microfluidic circuit in the reagent box.

It is noted that inside the microfluidic circuit of the cartridge, a protruding piercing member (not shown in the figures) is provided, which has a tip portion. The piercing member is arranged opposite the sample sealing membrane 6-7 and the diluting sealing membrane 6-8 with the pointed end of the piercing member facing the sample sealing membrane 6-7 and the diluting sealing membrane 6-8. Therefore, the thimble 12 can puncture the sample sealing films 6-7 and the dilution sealing films 6-8 by extruding the puncture piece under the condition of not directly contacting with the sample, and the microfluidic pipeline is always in an isolated state with the outside (equipment), thereby effectively avoiding the pollution of the sample to the equipment. In addition, PCR reaction in the kit is carried out in a sealed PCR reaction chamber, and the condition that products are volatilized into the cavity of the equipment through aerosol in the traditional PCR experiment process can not occur.

According to an embodiment of the present invention, as shown in fig. 11, the molecular detection system may further include: a linear guide rail 17 and a slide block 18, and the push rod 16 is arranged on the linear guide rail 17 through the slide block 18. The push rod 16 and the slider 18 may be fixed by a second nut 15. Thus, the push rod 16 moves linearly on the linear guide 17 under the driving of the third motor 13, thereby controlling the extension and contraction of the piston 6-4.

The detection device 400 is suitable for detecting the pretreated sample to be detected, and the temperature control device 500 is suitable for regulating and controlling the temperature of the sample to be detected. Specifically, the detection device includes a light path detection unit 19, and the temperature control device 500 includes a chip temperature circulation control 7. While the temperature control device 500 performs temperature cycle control of the sample in the PCR chamber 6-5, the detection device 400 can measure the amplification product in the PCR chamber 6-5 in real time to obtain the fluorescence signal of the amplification product, because the probes are distributed in the PCR chamber in advance, the amplified product can be hybridized with the probes to release fluorescent groups, and the fluorescent groups are increased along with the increase of the amplified product, resulting in an increasing fluorescence signal and eventually reaching the sensitivity range of the detection device 400, causing significant changes in the detected data, the cycle number of the corresponding amplification with significant changes in the data was defined as the CT value by the analysis software, the detection results in which the CT value appeared in a certain region were respectively defined as positive, and the rest is defined as negative, so that the data of the change of the fluorescence signal is converted into a detection result of positive and negative judgment. According to a specific example of the present invention, the detection result can be obtained by a PCR reaction on the 16S rRNA of the bacterium. The primers used in the PCR reaction included forward primer 63f (5'-CAGGCC TAA CAC ATG CAA GTC-3') and reverse primer 1387r (5'-GGG CGG TGT GTA CAA GGC-3').

According to the utility model discloses an embodiment, detection device's one end is passed through temperature control device and is linked to each other with the draw-in groove. As shown in FIGS. 7 and 8, by fixing the reagent cartridge in the card slot 6, the PCR chamber 6-5 in the reagent cartridge is adjacent to the temperature cycle control 7 in the temperature control device, so that the temperature control device can control the temperature of the PCR chamber 6-5.

According to an embodiment of the present invention, as shown in fig. 2, the molecular detection system further comprises a control system 20. The control system is respectively connected with the fixing device, the driving device, the detection device and the temperature control device and is suitable for controlling the fixing device, the driving device, the detection device and the temperature control device to work cooperatively so as to detect the sample to be detected.

In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above are not necessarily intended to refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples and features of different embodiments or examples described in this specification can be combined and combined by one skilled in the art without contradiction.

Although embodiments of the present invention have been shown and described, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made to the above embodiments by those of ordinary skill in the art without departing from the scope of the present invention.

Claims

1. A molecular detection system, comprising:

the device comprises a base body, wherein a fixing device, a driving device, a detection device, a temperature control device and a control system are arranged on the base body; wherein the content of the first and second substances,

the fixing device comprises a clamping groove, a first motor, a first nut, a first clamping component, a second clamping component and a connecting rod; the first motor is connected with the first clamping component through the first nut, the first clamping component is connected with the second clamping component through the connecting rod, and the clamping groove is formed between the first clamping component and the second clamping component;

the driving device comprises a second motor, a third motor, a thimble and a push rod; the second motor is connected with the thimble, and the third motor is connected with the push rod;

the control system is respectively connected with the fixing device, the driving device, the detection device and the temperature control device and is suitable for controlling the fixing device, the driving device, the detection device and the temperature control device to work cooperatively so as to detect a sample to be detected.

2. The molecular detection system of claim 1, wherein the base comprises a base, a support, and a sleeve coupled thereto.

3. The molecular detection system of claim 1, further comprising: and the pressure spring is arranged between the clamping groove and the second clamping component.

4. The molecular detection system of claim 1 or 3, further comprising: a first heat dissipation assembly; the first heat dissipation assembly is arranged below the clamping groove.

5. The molecular detection system of claim 1, further comprising: the push rod is arranged on the linear guide rail through the sliding block.

6. The molecular detection system of claim 1, wherein the detection device comprises an optical path detection unit and the temperature control device comprises a chip temperature cycling control.

7. The molecular detection system of claim 6, wherein one end of the detection device is connected to the card slot via the chip temperature cycling control.