CN114672511B - Application of corn ZmBES1/BZR1-3 gene in increasing plant seed yield - Google Patents
Application of corn ZmBES1/BZR1-3 gene in increasing plant seed yield Download PDFInfo
- Publication number
- CN114672511B CN114672511B CN202210270506.9A CN202210270506A CN114672511B CN 114672511 B CN114672511 B CN 114672511B CN 202210270506 A CN202210270506 A CN 202210270506A CN 114672511 B CN114672511 B CN 114672511B
- Authority
- CN
- China
- Prior art keywords
- zmbes1
- bzr1
- corn
- rice
- genes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Abstract
The invention discloses an application of corn ZmBES1/BZR1-3 genes in increasing plant seed yield, which utilizes corn seedling leaves to clone the corn ZmBES1/BZR1-3 genes, researches the functions of the genes from transgenic arabidopsis and phenotypic characteristics of rice, and provides new choices for rice high-yield breeding, rice economic benefit improvement and the like.
Description
Technical Field
The invention relates to application of a corn ZmBES1/BZR1-3 gene in increasing plant seed yield, belonging to the field of genetic engineering.
Background
Grain yield is always an important content of people's attention, and improving grain yield is significant for economic development and grain safety in China. The yield is a complex quantitative trait and is influenced by a plurality of factors such as plant type, spike grain, growth period and the like. The rice is a main grain crop, the formation of the single plant yield of the rice is mainly determined by the effective spike number, the grain number per spike, thousand grain weight and other characters, the thousand grain weight is the character with highest genetic power in yield forming factors, and is determined by grain shape and fullness, and the grain shape is determined by grain length, grain width and grain thickness. In the prior art, the application of some rice self genes to rice yield is mainly characterized in that GS3 is a first major QTL of grain length and grain weight obtained by a map-based cloning method, has weak effect on grain width and grain thickness, and simultaneously discovers that GS3 protein contains a plant-specific organ size regulation structural domain (OSR), and the structural domain is deleted or mutated to form large grains so as to push out the OSR to negatively regulate the grain shape and size. DEP1 is also a subunit of G protein, affecting not only spike shape but also grain shape. QTL GL7/GW7 encodes a LONGIFOLIA protein, and up-regulating GW7 expression slows down transverse cell division to promote the formation of elongated grains. qSW5/GW5 is a gene on chromosome 5 of rice for controlling grain width and grain weight of the rice, and GW5 can interact with polyubiquitin to regulate grain width and grain weight through ubiquitin proteasome pathway. GW2 negative regulation of rice grain width, and increasing GW2 expression can lead to narrowing of seed grain width.
In recent years, the rice yield has been gradually increased by introducing a single maize gene into rice plants, usually by increasing the photosynthetic rate of the rice, introducing a C4 photosynthetic-related enzyme into C3 crops such as the rice, for example, PEPCase is a key enzyme of the C4 pathway, successfully transferring the maize PEPC gene into the rice, and the CO2 compensation point and the photo respiration rate of the transgenic rice are remarkably reduced, the apparent net photosynthetic rate is increased, and the yield is also increased to different degrees. While other genes than photosynthesis are relatively less studied.
Disclosure of Invention
The invention overcomes the defects of the prior art, provides the application of the corn ZmBES1/BZR1-3 gene in increasing the plant seed yield, utilizes corn seedling leaves to clone the corn ZmBES1/BZR1-3 gene, researches the functions of the gene from the aspect of phenotype characteristics of transgenic arabidopsis and rice, and provides new choices for rice high-yield breeding, rice economic benefit improvement and the like.
Use of the maize ZmBES1/BZR1-3 gene, the nucleotide sequence of which is shown as SED ID No.1, for increasing seed yield in plants.
Use of the maize ZmBES1/BZR1-3 gene, the nucleotide sequence of which is shown as SED ID No.1, for improving the phenotype of plant seeds.
Further, the plant seed phenotype refers to seed grain length, grain width and thousand grain weight.
Further, the plant refers to one of Arabidopsis thaliana or rice.
A method for cultivating high-yield plants, which comprises the following steps: up-regulating the expression of ZmBES1/BZR1-3 gene of corn in plant to obtain the plant with over-expressed ZmBES1/BZR1-3 gene, so as to obtain high-yield plant.
Further, the method comprises the following specific steps: separating corn ZmBES1/BZR1-3 genes from corn seedling leaves, designing amplification primers of the corn ZmBES1/BZR1-3 genes, amplifying cDNA sequences of the corn ZmBES1/BZR1-3 genes by a PCR method, constructing a fungus-mediated 35S-ZmBES1/BZR1-3-eGFP vector by using the cDNA sequences of the ZmBES1/BZR1-3, transforming the vector into a target plant by using a agro-rod method, and improving the expression of the ZmBES1/BZR1-3 genes to obtain plants with over-expression of the ZmBES1/BZR1-3 genes, thereby obtaining high-yield plants.
Further, the nucleotide sequence of the corn ZmBES1/BZR1-3 gene is shown as SED ID NO. 1.
Further, the nucleotide sequences of the amplification primers of the corn ZmBES1/BZR1-3 genes are shown as SED ID No.2 and SED ID No. 3.
Further, the nucleotide sequence of the 35S-ZmBES1/BZR1-3-eGFP vector is shown as SED ID No. 10.
Further, the plant refers to one of Arabidopsis thaliana or rice.
The beneficial effects are that:
the invention provides a method for improving the yield of arabidopsis and rice seeds by using the ZmBES1/BZR1-3 genes of corn for the first time, and the method improves the yield of arabidopsis or rice by improving the expression of the ZmBES1/BZR1-3 genes of corn, thereby providing a novel molecular breeding approach for culturing high-yield rice good varieties and having wide application prospect. The invention can obviously increase the grain width, grain length and thousand grain weight of the arabidopsis or rice plants.
Drawings
FIG. 1 homozygous transgenic Arabidopsis seed phenotype.
FIG. 2 transgenic Arabidopsis seed grain length and grain width.
FIG. 3 transgenic rice seed phenotype.
FIG. 4 transgenic rice seed grain length and grain width.
FIG. 5 thousand kernel weight of homozygous transgenic rice.
Detailed Description
In order that those skilled in the art will better understand the technical solutions of the present application, the present invention will be further described with reference to examples, which are only a part of examples of the present application, but not all, and the present invention is not limited by the following examples.
Examples
1. Gene cloning and vector construction
Leaves of pentaleaf stage maize B73 seedlings were taken, flash ground with liquid nitrogen, and total RNA was extracted using total RNA extraction kit Trizol (TaKaRa) and according to the instructions thereof. By PrimeScript TM The RT regenant Kit (Takara) synthesizes cDNA using total RNA as a template. Cloning primers were designed using Primer 5.0 and the B73 cDNA was used as template, and specific PCR primers are shown in Table 1. Amplification was performed using PrimerStar (Takara Co.) high fidelity enzyme, and the PCR amplification reaction system was as shown in Table 2, and the temperature cycling procedure was: 94 ℃ for 3min;98 ℃,10s, and 10s of optimal annealing temperature; 72 ℃,20s;30 cycles; 72 ℃ for 5min; maintained at 4 ℃.
TABLE 1 ZmBES1/BZR1-3 cloning primers
TABLE 2 PCR amplification reaction System
1.1 transformation of Arabidopsis thaliana vector 35S-ZmBES1/BZR1-3-eGFP construction
According to the dicotyledon expression vector pCAMBIA2300-35S-eGFP multiple cloning site, the upstream primer is introduced into BamHI cleavage site, the downstream primer is introduced into SalI site, the specific sequence is as follows:
TABLE 3 construction of pCAMBIA2300-35S-eGFP vector primers
The reaction system shown in Table 1 was loaded and amplified. The temperature cycling program is as follows: 94 ℃ for 3min; 10s at 98 ℃,10s at the optimal annealing temperature, 20s at 72 ℃ and 30 cycles; 72 ℃ for 5min; maintained at 4 ℃.
And (5) recovering the PCR product. The recovered product, pCAMBIA2300-35S-eGFP plasmid, was subjected to double digestion and loaded according to the Table reaction system. Mixing the enzyme digestion system uniformly, placing the mixture in a water bath kettle at 30 ℃ for enzyme digestion for 6-8 hours, directly electrophoresis after the enzyme digestion product is added with a loading buffer solution, recovering the enzyme digestion product, using ClonExpress II One Step Cloning Kit (nuuzan), and adopting a reaction system as shown in Table 5, wherein the reaction conditions are as follows: 30mins at 37 ℃.
Table 4 double cleavage reaction System
Table 5 connection system
The ligation product was transformed into E.coli and subjected to colony PCR detection. After the recombinant plasmid is extracted and identified by restriction enzyme digestion, the plasmid bacterial liquid is sent to a sequencing company for DNA sequencing identification, and the plasmid with correct sequencing is transformed in the next step.
1.2 construction of transformed Rice vectors
The upstream primer was introduced into HindIII cleavage site and the downstream primer was introduced into BamHI site according to the monocot expression vector pCAMBIA1300-35S-eGFP multiple cloning site, the specific sequences of which are shown in Table 6. Amplification was performed using PrimerStar (Takara Co.) high fidelity enzyme and the PCR amplification reaction system was as shown in Table 2. The PCR product was then recovered.
TABLE 6 construction of pCAMBIA1300-35S-ZmBES1/BZR1-3-eGFP vector primers
The expression vector pCAMBIA1300-35S-eGFP plasmid was subjected to double cleavage using ClonExpress II One Step Cloning Kit (Nuo Weizan) with the reaction system as shown in Table 5 and the reaction conditions: 30mins at 37 ℃.
Table 44 shows the reaction system for sample addition. Mixing the enzyme digestion system uniformly, placing the mixture in a water bath kettle at 30 ℃ for enzyme digestion for 6-8 hours, directly electrophoresis after the enzyme digestion product is added with a loading buffer solution, recovering the enzyme digestion product, using ClonExpress II One Step Cloning Kit (nuuzan), and adopting a reaction system as shown in Table 5, wherein the reaction conditions are as follows: 30mins at 37 ℃.
The ligation product was transformed into E.coli and subjected to colony PCR detection. After the recombinant plasmid is extracted and identified by restriction enzyme digestion, the plasmid bacterial liquid is sent to a sequencing company for DNA sequence sequencing identification, and the plasmid with correct sequencing is transformed in the next step of rice.
2. Arabidopsis thaliana, rice transformation and positive identification
2.1 transformation of Arabidopsis thaliana by Ficus infestations
Exploring the function of maize ZmBES1/BZR1-3, we transformed the pCAMBIA2300-35S-ZmBES1/BZR1-3-eGFP vector into an Arabidopsis BES1 mutant (BES 1-D) using Agrobacterium-mediated batting.
2.1.1 inflorescence dip-dyeing transformation
(1) The agrobacterium containing the recombinant plasmid is streaked on a YEP plate containing Kana and Rif, and cultured for 2-3 d at 28 ℃.
(2) Single colonies of Agrobacterium were picked and inoculated in 3mL of YEP liquid medium containing Kana and Rif, at 28℃at 200r/min, and cultured overnight with shaking.
(3) Inoculating 1mL of overnight-cultured initial Agrobacterium solution in 100mL of liquid YEP medium (containing Kana and Rif), and shake culturing at 28deg.C to bacterial solution OD 600 The value is 1.2-1.5.
(4) Centrifuging at 4deg.C at 5000r/min for 10min to collect cells, suspending thallus with 5% sucrose solution, and adjusting OD 600 The value is 0.8-1.0, and the surfactant silwet L-77 is added according to the proportion of 1-2 percent.
(5) Culturing Arabidopsis BES1 mutant by using nutrient soil for planting, pruning to form fruit pod and flower, immersing inflorescence for 1.5-2.0 min by using the infection liquid, and culturing in dark for 10h.
(6) Culturing for 3-4 weeks under proper conditions, collecting seeds, and performing the next screening treatment.
2.1.2 screening of transgenic lines
(1) The harvested infected seeds were aliquoted into 1.5mL EP tubes and immersed in 500. Mu.L of 70% alcohol for 30s.
(2) After the alcohol is sucked off, the centrifuge tube is continuously shaken during the soaking with 500 mu L of 10% sodium hypochlorite for 5-10 min until the seeds are fully contacted with the sodium hypochlorite.
(3) Washing the seeds with sterilized water for 4-6 times, and discarding the upper ddH layer after the seeds subside 2 O; 200. Mu.L of 0.1% agar water suspended seeds.
(4) Seeds were sown on demand in 1/2MS medium containing 40mg/L kanamycin (Kana) for cultivation.
(5) Repeating the above steps to obtain transformed Arabidopsis thaliana T 2 After seed disinfection, the seeds were sown on 1/2MS solid medium containing kanamycin, and after 7-8 d, all the plants were green and robust, i.e., homozygous transformants, whereas the non-homozygous transformants exhibited 3:1 segregation (ratio of normal growth to yellowing dead plants). Lay a homozygous T 3 The seed substitutes were used for further analysis.
2.2. Rice transformation
The constructed vector was sent to the Oryza sativa wild type "Nip" from Oryza sativa Biotechnology, nanj.
2.2.1 genomic PCR identification
DNA extraction and PCR identification of the obtained positive lines
(1) 100mg of fresh plant tissue is taken and added with liquid nitrogen for full grinding. 400. Mu.L of buffer FP1 and 6. Mu.L of RNaseA (10 mg/mL) were added thereto, vortexed for 1min, and left at room temperature for 10min.
(2) 130. Mu.L of buffer FP2 was added, thoroughly mixed, centrifuged at 12000r/min (13400 Xg) for 5min with vortexing, and the supernatant was transferred to a new centrifuge tube.
(3) The optional steps are as follows: the supernatant was centrifuged again at 12000r/min (13400 Xg) for 5min and the supernatant was transferred to a new centrifuge tube.
(4) 0.7 volumes of isopropanol was added to the supernatant and mixed well, at which point flocculent genomic DNA appeared. Centrifuge at 12000r/min (13400 Xg) for 2min, discard supernatant and leave pellet.
(5) 600. Mu.L of 70% ethanol was added thereto, vortexed and shaken for 5s, centrifuged at 12000r/min (13400 Xg) for 2min, and the supernatant was discarded.
(6) Repeating the step (6).
(7) And (5) uncovering and inverting, and airing residual ethanol thoroughly at room temperature for 5-10 min.
(8) Adding proper amount of elution buffer TE, dissolving DNA in water bath at 65 deg.c for 10-60 min, and mixing to obtain DNA solution.
(9) PCR detection was performed using the extracted genomic DNA as a template using PCR primers as shown in Table 8, with a procedure of 94℃for 3min;94℃for 30s, an optimum annealing temperature of 30s,72℃for 60s/kb,35 cycles; 72 ℃ for 5min; preserving at 4 ℃.
Table 7 transgenic rice detection primers
(10) Harvesting transformed Rice seed T 2 For the generation, seeds of all positive lines of the offspring were detected after sowing and used for further analysis.
3. Transgenic line yield analysis
3.1 identification of Arabidopsis seed phenotype
Homozygous transgenic Arabidopsis lines L3-1, L3-10 and mutant (bes 1-D) were sterilized with 5% sodium hypochlorite solution, sown on 1/2MS medium, 15D later transplanted to nutrient-containing soil: vermiculite = 3:1 soil in a flowerpot of 50mm x 50mm, 4 plants were sown per pot, cultivated under the same conditions for direct harvest, and seed grain length and grain width were measured.
3.2 characterization of Rice seed phenotype
Homozygous transgenic rice lines L3-1 and L3-10 and wild type (NIP) were sown in paddy fields, and the seeds were cultivated under the same conditions for direct harvest, and seed grain length, grain width and thousand grain weight were measured.
4. Transgenic line yield analysis
4.1 analysis of transgenic Arabidopsis yield
Through resistance screening, the stable expression of the target gene ZmBES1/BZR1-3 in arabidopsis is determined, and the grain length and grain width of the seed of homozygous transgenic arabidopsis are analyzed (figure 1), so that the grain length of the over-expression strain L3-10 seed is obviously increased, and the grain widths of the L3-1 seed and the L3-10 seed are obviously increased (figure 2, and respectively represent p <0.05 and p < 0.01).
4.2 analysis of transgenic Rice yield
And detecting the target gene by PCR to determine a positive strain. Analysis of the grain length, grain width and thousand grain weight of positive lines (fig. 3) showed that the grain length of the overexpressing lines R3-3 and R3-4 was extremely significantly increased with no significant change in grain width (fig. 4, and p <0.05, p <0.01, respectively). Thousand kernel weight was significantly increased compared to control NIP (fig. 5, and represent p <0.05, p <0.01, respectively).
SEQUENCE LISTING
<110> Sichuan university of agriculture
<120> use of maize ZmBES1/BZR1-3 genes for increasing seed yield in plants
<130> 2022
<160> 10
<170> PatentIn version 3.3
<210> 1
<211> 1311
<212> DNA
<213> sequence
<400> 1
atggagggag gggtaggagc gggaggaagt cggggtgatg agggttgggc tagggcagga 60
gacggggacg ggaagaacgg gaagcagggg acggtgcgcg agcgcgagcc gtcgtggcgt 120
gagcgggaga acaacaacca gcgccgggag cgccgtcgtc gggtggtcgc gtcgaggatc 180
ttcgccgggc tgcgcagata cggcaactac gccctcccca ggcactacga caacaacgtc 240
gtgctcatgg cgctctgcga ggaggccggc tggaccgtgg aggccgacgg caccacctac 300
cgcaggggag gaaagcctcc ggcaggtgat cagcatatgg ccgacattgg cgggtctgct 360
gctccggtga cccaccaggg agcttccgac ggtgggggca gcgctagcgg cggagccgac 420
cccgttccgg cgtggctcaa gaacctgtcc aagcagctca gcgactcgtc gtaccccaac 480
tacttcgcga gctcctccag ctccaacgcg ccggcgacgc cgcacaacgg ctcgccatcc 540
tcgtccccgc ggcgcctccg caagatggcg cgctactcct ccccgccgtc caccccgccg 600
ccgagcccgg cgagggcctc cgacatgctg ccgccatggg ccatacgcga tggtggcggc 660
agccgctact ccttccaggc gttctcattc cacgggagcg gcgatgggga gcaggcgggc 720
gcgaaggagg gggagggaaa aggaggagga gaaggtgcaa cgccacgagg aagaggttgc 780
agccacatat gccggggtgc aagccatgag gaggaggtct cgagcataga tgatggggtg 840
caggaccatg agaaggtcca ggcggatgac gagaccatcg aggctcctgc ctttcgacgg 900
aggggcactc ggaagggcca ctttgtcctt cctttgtcag agcctgcaca atcagatgct 960
cggagacgct atcgtgtgga acctgaggct caggagcatg ccgaccaagt attagagaaa 1020
aacgtgaaga aggttgtgaa tgtgtacatg aagcgtcgag gatattctat aaataacttt 1080
cggcaatatt cagatgtcta cttgaatgtg gaccagtaca tagagataca ggagtcttac 1140
aggcaggaga tagttaggaa atattctatg tttatcggtg tgatcgattc gacggagtta 1200
cggtctcgtg ggctgcttct tcgttgcagg caggttatgg tagcagttgt tagtcggggg 1260
ctccgagcat gggaggagtc tatgagggag cagcaagaaa ggttttcgtg a 1311
<210> 2
<211> 20
<212> DNA
<213> artificial sequence
<400> 2
<210> 3
<211> 22
<212> DNA
<213> artificial sequence
<400> 3
tcacgaaaac ctttcttgct gc 22
<210> 4
<211> 44
<212> DNA
<213> artificial sequence
<400> 4
tcctctagag tcgacatgga gggaggggta ggagcgggag gaag 44
<210> 5
<211> 47
<212> DNA
<213> artificial sequence
<400> 5
caccatggta ctagtgtcga ccgaaaacct ttcttgctgc tccctca 47
<210> 6
<211> 41
<212> DNA
<213> artificial sequence
<400> 6
tggagaggac agcccaagct tatggaggga ggggtaggag c 41
<210> 7
<211> 43
<212> DNA
<213> artificial sequence
<400> 7
ctcaccatga ccggtggatc cgaaaacctt tcttgctgct ccc 43
<210> 8
<211> 20
<212> DNA
<213> artificial sequence
<400> 8
<210> 9
<211> 22
<212> DNA
<213> artificial sequence
<400> 9
tcacgaaaac ctttcttgct gc 22
<210> 10
<211> 2840
<212> DNA
<213> artificial sequence
<400> 10
aattcccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg 60
cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat 120
ggtggagcac gacacgcttg tctactccaa aaatatcaaa gatacagtct cagaagacca 180
aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg 240
cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg 300
ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa 360
agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc 420
aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta 480
tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga ggacagggta 540
cccggggatc ctctagagtc gacatggagg gaggggtagg agcgggagga agtcggggtg 600
atgagggttg ggctagggca ggagacgggg acgggaagaa cgggaagcag gggacggtgc 660
gcgagcgcga gccgtcgtgg cgtgagcggg agaacaacaa ccagcgccgg gagcgccgtc 720
gtcgggtggt cgcgtcgagg atcttcgccg ggctgcgcag atacggcaac tacgccctcc 780
ccaggcacta cgacaacaac gtcgtgctca tggcgctctg cgaggaggcc ggctggaccg 840
tggaggccga cggcaccacc taccgcaggg gaggaaagcc tccggcaggt gatcagcata 900
tggccgacat tggcgggtct gctgctccgg tgacccacca gggagcttcc gacggtgggg 960
gcagcgctag cggcggagcc gaccccgttc cggcgtggct caagaacctg tccaagcagc 1020
tcagcgactc gtcgtacccc aactacttcg cgagctcctc cagctccaac gcgccggcga 1080
cgccgcacaa cggctcgcca tcctcgtccc cgcggcgcct ccgcaagatg gcgcgctact 1140
cctccccgcc gtccaccccg ccgccgagcc cggcgagggc ctccgacatg ctgccgccat 1200
gggccatacg cgatggtggc ggcagccgct actccttcca ggcgttctca ttccacggga 1260
gcggcgatgg ggagcaggcg ggcgcgaagg agggggaggg aaaaggagga ggagaaggtg 1320
caacgccacg aggaagaggt tgcagccaca tatgccgggg tgcaagccat gaggaggagg 1380
tctcgagcat agatgatggg gtgcaggacc atgagaaggt ccaggcggat gacgagacca 1440
tcgaggctcc tgcctttcga cggaggggca ctcggaaggg ccactttgtc cttcctttgt 1500
cagagcctgc acaatcagat gctcggagac gctatcgtgt ggaacctgag gctcaggagc 1560
atgccgacca agtattagag aaaaacgtga agaaggttgt gaatgtgtac atgaagcgtc 1620
gaggatattc tataaataac tttcggcaat attcagatgt ctacttgaat gtggaccagt 1680
acatagagat acaggagtct tacaggcagg agatagttag gaaatattct atgtttatcg 1740
gtgtgatcga ttcgacggag ttacggtctc gtgggctgct tcttcgttgc aggcaggtta 1800
tggtagcagt tgttagtcgg gggctccgag catgggagga gtctatgagg gagcagcaag 1860
aaaggttttc gactagtacc atggtgagca agggcgagga gctgttcacc ggggtggtgc 1920
ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg 1980
gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc 2040
tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc 2100
gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg 2160
tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga 2220
agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg 2280
acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca 2340
tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg 2400
acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg 2460
tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg 2520
agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca 2580
tggacgagct gtacaagtaa ctgcaggcat gccagggctc tcaatggagt ttgaatcaaa 2640
tcttccagct gctttaatga gatatgcgag acgcctatga tcgcatgata tttgctttca 2700
attctgttgt gcacgttgta aaaaacctga gcatgtgtag ctcagatcct taccgccggt 2760
ttcggttcat tctaatgaat atatcacccg ttactatcgt atttttatga ataatattct 2820
ccgttcaatt tactgattga 2840
Claims (5)
1. The application of the corn ZmBES1/BZR1-3 gene in increasing thousand seed weight of rice seeds is characterized in that the nucleotide sequence of the corn ZmBES1/BZR1-3 gene is shown as SED ID NO. 1.
2. The application of the corn ZmBES1/BZR1-3 gene in improving plant seed phenotype is characterized in that the nucleotide sequence of the corn ZmBES1/BZR1-3 gene is shown as SED ID NO. 1; the plant seed phenotype refers to the grain length and grain width of arabidopsis thaliana or the grain length of rice.
3. A method for cultivating high-yield rice is characterized in that the expression of ZmBES1/BZR1-3 genes of corn in the rice is up-regulated to obtain plants with over-expressed ZmBES1/BZR1-3 genes, so that the high-yield rice is obtained; the nucleotide sequence of the corn ZmBES1/BZR1-3 gene is shown as SED ID NO. 1; the high yield refers to increasing thousand seed weight of rice seeds.
4. A method according to claim 3, characterized by the specific steps of: separating corn ZmBES1/BZR1-3 genes from corn seedling leaves, designing amplification primers of the corn ZmBES1/BZR1-3 genes, amplifying cDNA sequences of the corn ZmBES1/BZR1-3 genes by a PCR method, constructing a 35S-ZmBES1/BZR1-3-eGFP vector by using the cDNA sequences of the ZmBES1/BZR1-3, transforming the vector into target rice by using an agrobacterium-mediated method, improving the expression of the ZmBES1/BZR1-3 genes, and obtaining plants with over-expressed ZmBES1/BZR1-3 genes, thereby obtaining high-yield rice.
5. The method of claim 3, wherein the nucleotide sequences of the amplification primers of the maize ZmBES1/BZR1-3 gene are shown as SED ID No.2 and SED ID No. 3.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210270506.9A CN114672511B (en) | 2022-03-18 | 2022-03-18 | Application of corn ZmBES1/BZR1-3 gene in increasing plant seed yield |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210270506.9A CN114672511B (en) | 2022-03-18 | 2022-03-18 | Application of corn ZmBES1/BZR1-3 gene in increasing plant seed yield |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114672511A CN114672511A (en) | 2022-06-28 |
| CN114672511B true CN114672511B (en) | 2023-04-25 |
Family
ID=82074393
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210270506.9A Active CN114672511B (en) | 2022-03-18 | 2022-03-18 | Application of corn ZmBES1/BZR1-3 gene in increasing plant seed yield |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114672511B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115851753B (en) * | 2022-07-06 | 2024-03-15 | 四川农业大学 | Application of corn ZmBES1/BZR1-1 gene in improving plant yield |
| CN116732088B (en) * | 2023-08-10 | 2023-11-07 | 浙江大学海南研究院 | Application of PpyBZR2 gene in promoting pear dormancy bud germination |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105087633A (en) * | 2014-04-29 | 2015-11-25 | 中国科学院上海生命科学研究院 | Gene for regulating plant height, tiller number and leaf inclination and application thereof |
| CN105420245A (en) * | 2015-11-19 | 2016-03-23 | 中国农业科学院生物技术研究所 | Salt-stress induced gene OsEAR1 of plants and encoding protein and application thereof |
| CN112119163A (en) * | 2018-02-16 | 2020-12-22 | 首尔大学校产学协力团 | Transgenic plants with increased yield |
| CN112941087A (en) * | 2021-04-06 | 2021-06-11 | 四川农业大学 | Application of corn ZmBES1/BZR1-2 gene in improving plant drought tolerance |
| CN114480422A (en) * | 2022-02-09 | 2022-05-13 | 四川农业大学 | Application of corn ZmBES1/BZR1-9 gene in breeding early flowering plants |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8008542B2 (en) * | 2007-01-10 | 2011-08-30 | The Salk Institute For Biological Studies | Compositions, cells, and plants that include BKI1, a negative regulator of BRI1-mediated BR signaling |
| WO2011048009A1 (en) * | 2009-10-22 | 2011-04-28 | Basf Plant Science Company Gmbh | Plants having enhanced yield-related traits and a method for making the same |
| CA2966102A1 (en) * | 2014-11-04 | 2016-05-12 | Agresearch Limited | Methods for plant improvement |
| CN112226455B (en) * | 2019-06-27 | 2022-05-10 | 中国科学院植物研究所 | Rice grain length and grain weight related protein, and coding gene and application thereof |
| CN113121662B (en) * | 2019-12-31 | 2022-11-01 | 中国科学院植物研究所 | Application of cotton GhBZR3 protein and coding gene thereof in regulating plant growth and development |
| CN111440804B (en) * | 2020-04-30 | 2021-03-30 | 四川农业大学 | Application of corn ZmBES1/BZR1-5 gene in cultivation of large-grain plants |
| CN114480431B (en) * | 2022-03-30 | 2022-09-20 | 四川农业大学 | Application of corn ZmBES1/BZR1-10 gene in improving drought tolerance and yield of plants |
-
2022
- 2022-03-18 CN CN202210270506.9A patent/CN114672511B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105087633A (en) * | 2014-04-29 | 2015-11-25 | 中国科学院上海生命科学研究院 | Gene for regulating plant height, tiller number and leaf inclination and application thereof |
| CN105420245A (en) * | 2015-11-19 | 2016-03-23 | 中国农业科学院生物技术研究所 | Salt-stress induced gene OsEAR1 of plants and encoding protein and application thereof |
| CN112119163A (en) * | 2018-02-16 | 2020-12-22 | 首尔大学校产学协力团 | Transgenic plants with increased yield |
| CN112941087A (en) * | 2021-04-06 | 2021-06-11 | 四川农业大学 | Application of corn ZmBES1/BZR1-2 gene in improving plant drought tolerance |
| CN114480422A (en) * | 2022-02-09 | 2022-05-13 | 四川农业大学 | Application of corn ZmBES1/BZR1-9 gene in breeding early flowering plants |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114672511A (en) | 2022-06-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN114672511B (en) | Application of corn ZmBES1/BZR1-3 gene in increasing plant seed yield | |
| CN114480431B (en) | Application of corn ZmBES1/BZR1-10 gene in improving drought tolerance and yield of plants | |
| CN106868021B (en) | Gene OsNAC1 for controlling rice seed size and application thereof | |
| CN111440804B (en) | Application of corn ZmBES1/BZR1-5 gene in cultivation of large-grain plants | |
| CN109837296B (en) | New salt-tolerant drought-tolerant function of corn gene ZmNAC77 and application thereof | |
| CN112626084B (en) | Strawberry MYB transcription factor FvMYB24 gene, expression protein and application | |
| CN114480422B (en) | Application of corn ZmBES1/BZR1-9 gene in breeding early flowering plants | |
| CN113337522B (en) | Application of cotton GhNFYC4 gene in promoting plant flowering | |
| CN114807168A (en) | Mung bean VrMIB1 gene and application thereof | |
| CN109971765B (en) | Corn gene ZmNAC77 for regulating and controlling contents of fatty acids and starch in arabidopsis thaliana and application thereof | |
| CN108588069B (en) | Precursor gene of mulberry miR171a and application thereof in enhancing salt tolerance of plants | |
| CN108017697B (en) | Plant tillering angle related protein HSFA2D, and coding gene and application thereof | |
| CN115851753B (en) | Application of corn ZmBES1/BZR1-1 gene in improving plant yield | |
| CN112501181A (en) | Rice stress resistance related gene OsTZF7 and encoding protein and application thereof | |
| CN116656698B (en) | Application of corn gene Zm00001d018037 in improving drought resistance of monocotyledonous crops | |
| CN113584051B (en) | Application of GhGAI gene in regulation and control of plant flowering | |
| CN117511994A (en) | Application of corn ZmBES1/BZR1-4 gene in increasing seed yield | |
| CN114540382B (en) | Tobacco cadmium transport gene NtPLA1 and application thereof | |
| CN112877337B (en) | Application of rape BnaA09WRKY6 gene in promotion of bolting and flowering of cruciferous plants | |
| CN111607604B (en) | Application of cotton GHPSAT2 gene in promoting flowering of plants | |
| CN117660485A (en) | Application of Arabidopsis ERF012 gene in regulation of seed germination | |
| CN110760522B (en) | AK209 gene and its coded protein and application in resisting stress and increasing yield | |
| CN117286149A (en) | Application of over-expressed GhCIB1 gene in promotion of cotton flowering | |
| CN106544347B (en) | A method of regulation vegetable seeds dormancy time | |
| CN117230082A (en) | Method for regulating and controlling flowering time of plant, biological material and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |