CN114667344A - 具有增强的dna产生的经修饰的细菌逆转录元件 - Google Patents

具有增强的dna产生的经修饰的细菌逆转录元件 Download PDF

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CN114667344A
CN114667344A CN202080078530.1A CN202080078530A CN114667344A CN 114667344 A CN114667344 A CN 114667344A CN 202080078530 A CN202080078530 A CN 202080078530A CN 114667344 A CN114667344 A CN 114667344A
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engineered
sequence
gene
msd
cell
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CN202080078530.1A
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Chinese (zh)
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塞特·希普曼
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J David Gladstone Institutes
Harvard University
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J David Gladstone Institutes
Harvard University
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Priority to CN202510075807.XA priority Critical patent/CN119859624A/zh
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07049—RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
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  • General Engineering & Computer Science (AREA)
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  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
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  • General Health & Medical Sciences (AREA)
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  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CN202080078530.1A 2019-09-12 2020-09-11 具有增强的dna产生的经修饰的细菌逆转录元件 Pending CN114667344A (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202510075807.XA CN119859624A (zh) 2019-09-12 2020-09-11 具有增强的dna产生的经修饰的细菌逆转录元件

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962899625P 2019-09-12 2019-09-12
US62/899,625 2019-09-12
PCT/US2020/050323 WO2021050822A1 (en) 2019-09-12 2020-09-11 Modified bacterial retroelement with enhanced dna production

Related Child Applications (1)

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CN202510075807.XA Division CN119859624A (zh) 2019-09-12 2020-09-11 具有增强的dna产生的经修饰的细菌逆转录元件

Publications (1)

Publication Number Publication Date
CN114667344A true CN114667344A (zh) 2022-06-24

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CN202510075807.XA Pending CN119859624A (zh) 2019-09-12 2020-09-11 具有增强的dna产生的经修饰的细菌逆转录元件
CN202080078530.1A Pending CN114667344A (zh) 2019-09-12 2020-09-11 具有增强的dna产生的经修饰的细菌逆转录元件

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CN202510075807.XA Pending CN119859624A (zh) 2019-09-12 2020-09-11 具有增强的dna产生的经修饰的细菌逆转录元件

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US (3) US20220307007A1 (enExample)
EP (1) EP4028512A4 (enExample)
JP (2) JP2022548062A (enExample)
KR (1) KR20220081988A (enExample)
CN (2) CN119859624A (enExample)
AU (1) AU2020346880B2 (enExample)
BR (1) BR112022004453A2 (enExample)
CA (1) CA3154384A1 (enExample)
IL (1) IL291128A (enExample)
MX (1) MX2022003091A (enExample)
WO (1) WO2021050822A1 (enExample)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4384616A4 (en) * 2021-08-11 2025-08-27 Univ Leland Stanford Junior HIGH-THROUGHPUT PRECISION GENOME EDITING IN HUMAN CELLS
EP4426832A1 (en) * 2021-11-03 2024-09-11 The J. David Gladstone Institutes, A Testamentary Trust Established under The Will of J. David Gladstone Precise genome editing using retrons
US11866728B2 (en) 2022-01-21 2024-01-09 Renagade Therapeutics Management Inc. Engineered retrons and methods of use
US20250223586A1 (en) * 2022-03-25 2025-07-10 The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Methods of assessing engineered retron activity, and uses thereof
WO2023183627A1 (en) * 2022-03-25 2023-09-28 The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Gladstone Production of reverse transcribed dna (rt-dna) using a retron reverse transcriptase from exogenous rna
US20250215422A1 (en) * 2022-03-25 2025-07-03 The J. David Gladstone Institutes, a testamentary trust established under the Will of J. David Glads RT-DNA Fidelity and Retron Genome Editing
US20250230429A1 (en) * 2022-04-07 2025-07-17 The J. David Gladstone Institutes, a testamentary trust established under the Will of J. David Glads Continuous Multiplexed Phage Genome Engineering Using a Retron Editing Template
WO2024044673A1 (en) * 2022-08-24 2024-02-29 The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Gladstone Dual cut retron editors for genomic insertions and deletions
WO2025063625A1 (ko) * 2023-09-18 2025-03-27 포항공과대학교 산학협력단 Dna 결합 단백질 특이적 결합 부위를 포함하는 조작된 레트론 및 이의 용도
KR20250042094A (ko) 2023-09-18 2025-03-26 포항공과대학교 산학협력단 Dna 결합 단백질 특이적 결합 부위를 포함하는 조작된 레트론 및 이의 용도
WO2025128871A2 (en) 2023-12-13 2025-06-19 Renagade Therapeutics Management Inc. Lipid nanoparticles comprising coding rna molecules for use in gene editing and as vaccines and therapeutic agents

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050250207A1 (en) * 2002-06-05 2005-11-10 Rozwadowski Kevin L Retrons for gene targeting
WO2018049168A1 (en) * 2016-09-09 2018-03-15 The Board Of Trustees Of The Leland Stanford Junior University High-throughput precision genome editing
US20180127759A1 (en) * 2016-10-28 2018-05-10 Massachusetts Institute Of Technology Dynamic genome engineering
WO2018191525A1 (en) * 2017-04-12 2018-10-18 President And Fellows Of Harvard College Method of recording multiplexed biological information into a crispr array using a retron

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5434070A (en) * 1989-02-24 1995-07-18 The University Of Medicine And Dentistry Of New Jersey Reverse transcriptases from Escherichia coli and Myxococcus xanthus
US20050026109A1 (en) * 2003-07-30 2005-02-03 Buchanan L. Stephen Multi-tapered dental files
US20170204399A1 (en) * 2014-08-15 2017-07-20 Massachusetts Institute Of Technology Genomically-encoded memory in live cells
KR101922989B1 (ko) * 2016-05-13 2018-11-28 연세대학교 산학협력단 ＣＲＩＳＰＲ／Ｒｅｔｒｏｎ 시스템을 이용한 유전체상의 치환 변이 생성과 추적 방법

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050250207A1 (en) * 2002-06-05 2005-11-10 Rozwadowski Kevin L Retrons for gene targeting
WO2018049168A1 (en) * 2016-09-09 2018-03-15 The Board Of Trustees Of The Leland Stanford Junior University High-throughput precision genome editing
US20180127759A1 (en) * 2016-10-28 2018-05-10 Massachusetts Institute Of Technology Dynamic genome engineering
WO2018191525A1 (en) * 2017-04-12 2018-10-18 President And Fellows Of Harvard College Method of recording multiplexed biological information into a crispr array using a retron

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Publication number Publication date
WO2021050822A1 (en) 2021-03-18
IL291128A (en) 2022-05-01
JP2022548062A (ja) 2022-11-16
US20220307007A1 (en) 2022-09-29
KR20220081988A (ko) 2022-06-16
JP2024102277A (ja) 2024-07-30
MX2022003091A (es) 2022-06-08
AU2020346880B2 (en) 2025-04-03
EP4028512A1 (en) 2022-07-20
AU2020346880A1 (en) 2022-04-21
US20230125704A1 (en) 2023-04-27
CA3154384A1 (en) 2021-03-18
CN119859624A (zh) 2025-04-22
US20250188442A1 (en) 2025-06-12
BR112022004453A2 (pt) 2022-06-21
EP4028512A4 (en) 2023-09-20

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