CA3154384A1 - Modified bacterial retroelement with enhanced dna production - Google Patents

Modified bacterial retroelement with enhanced dna production Download PDF

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Publication number
CA3154384A1
CA3154384A1 CA3154384A CA3154384A CA3154384A1 CA 3154384 A1 CA3154384 A1 CA 3154384A1 CA 3154384 A CA3154384 A CA 3154384A CA 3154384 A CA3154384 A CA 3154384A CA 3154384 A1 CA3154384 A1 CA 3154384A1
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CA
Canada
Prior art keywords
retron
engineered
sequence
gene
cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3154384A
Other languages
English (en)
French (fr)
Inventor
Seth SHIPMAN
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
J David Gladstone Institutes
Harvard University
Original Assignee
J David Gladstone Institutes
Harvard University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by J David Gladstone Institutes, Harvard University filed Critical J David Gladstone Institutes
Publication of CA3154384A1 publication Critical patent/CA3154384A1/en
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07049—RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CA3154384A 2019-09-12 2020-09-11 Modified bacterial retroelement with enhanced dna production Pending CA3154384A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962899625P 2019-09-12 2019-09-12
US62/899,625 2019-09-12
PCT/US2020/050323 WO2021050822A1 (en) 2019-09-12 2020-09-11 Modified bacterial retroelement with enhanced dna production

Publications (1)

Publication Number Publication Date
CA3154384A1 true CA3154384A1 (en) 2021-03-18

Family

ID=74865645

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3154384A Pending CA3154384A1 (en) 2019-09-12 2020-09-11 Modified bacterial retroelement with enhanced dna production

Country Status (11)

Country Link
US (3) US20220307007A1 (enExample)
EP (1) EP4028512A4 (enExample)
JP (2) JP2022548062A (enExample)
KR (1) KR20220081988A (enExample)
CN (2) CN119859624A (enExample)
AU (1) AU2020346880B2 (enExample)
BR (1) BR112022004453A2 (enExample)
CA (1) CA3154384A1 (enExample)
IL (1) IL291128A (enExample)
MX (1) MX2022003091A (enExample)
WO (1) WO2021050822A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11866728B2 (en) 2022-01-21 2024-01-09 Renagade Therapeutics Management Inc. Engineered retrons and methods of use

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4384616A4 (en) * 2021-08-11 2025-08-27 Univ Leland Stanford Junior HIGH-THROUGHPUT PRECISION GENOME EDITING IN HUMAN CELLS
EP4426832A1 (en) * 2021-11-03 2024-09-11 The J. David Gladstone Institutes, A Testamentary Trust Established under The Will of J. David Gladstone Precise genome editing using retrons
US20250223586A1 (en) * 2022-03-25 2025-07-10 The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Methods of assessing engineered retron activity, and uses thereof
WO2023183627A1 (en) * 2022-03-25 2023-09-28 The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Gladstone Production of reverse transcribed dna (rt-dna) using a retron reverse transcriptase from exogenous rna
US20250215422A1 (en) * 2022-03-25 2025-07-03 The J. David Gladstone Institutes, a testamentary trust established under the Will of J. David Glads RT-DNA Fidelity and Retron Genome Editing
US20250230429A1 (en) * 2022-04-07 2025-07-17 The J. David Gladstone Institutes, a testamentary trust established under the Will of J. David Glads Continuous Multiplexed Phage Genome Engineering Using a Retron Editing Template
WO2024044673A1 (en) * 2022-08-24 2024-02-29 The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Gladstone Dual cut retron editors for genomic insertions and deletions
WO2025063625A1 (ko) * 2023-09-18 2025-03-27 포항공과대학교 산학협력단 Dna 결합 단백질 특이적 결합 부위를 포함하는 조작된 레트론 및 이의 용도
KR20250042094A (ko) 2023-09-18 2025-03-26 포항공과대학교 산학협력단 Dna 결합 단백질 특이적 결합 부위를 포함하는 조작된 레트론 및 이의 용도
WO2025128871A2 (en) 2023-12-13 2025-06-19 Renagade Therapeutics Management Inc. Lipid nanoparticles comprising coding rna molecules for use in gene editing and as vaccines and therapeutic agents

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5434070A (en) * 1989-02-24 1995-07-18 The University Of Medicine And Dentistry Of New Jersey Reverse transcriptases from Escherichia coli and Myxococcus xanthus
EP1517992B1 (en) * 2002-06-05 2013-08-21 Her Majesty the Queen in Right of Canada, as represented by the Minister of Agriculture and Agri-Food Retrons for gene targeting
US20050026109A1 (en) * 2003-07-30 2005-02-03 Buchanan L. Stephen Multi-tapered dental files
US20170204399A1 (en) * 2014-08-15 2017-07-20 Massachusetts Institute Of Technology Genomically-encoded memory in live cells
KR101922989B1 (ko) * 2016-05-13 2018-11-28 연세대학교 산학협력단 ＣＲＩＳＰＲ／Ｒｅｔｒｏｎ 시스템을 이용한 유전체상의 치환 변이 생성과 추적 방법
ES2985812T3 (es) * 2016-09-09 2024-11-07 The Board Of Trustees Of The Leland Stanfordjunior Univ Edición genómica de precisión de alto rendimiento
WO2018081535A2 (en) * 2016-10-28 2018-05-03 Massachusetts Institute Of Technology Dynamic genome engineering
WO2018191525A1 (en) * 2017-04-12 2018-10-18 President And Fellows Of Harvard College Method of recording multiplexed biological information into a crispr array using a retron

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11866728B2 (en) 2022-01-21 2024-01-09 Renagade Therapeutics Management Inc. Engineered retrons and methods of use
US12054739B2 (en) 2022-01-21 2024-08-06 Renagade Therapeutics Management Inc. Engineered retrons and methods of use

Also Published As

Publication number Publication date
WO2021050822A1 (en) 2021-03-18
IL291128A (en) 2022-05-01
JP2022548062A (ja) 2022-11-16
US20220307007A1 (en) 2022-09-29
KR20220081988A (ko) 2022-06-16
JP2024102277A (ja) 2024-07-30
MX2022003091A (es) 2022-06-08
AU2020346880B2 (en) 2025-04-03
EP4028512A1 (en) 2022-07-20
AU2020346880A1 (en) 2022-04-21
US20230125704A1 (en) 2023-04-27
CN119859624A (zh) 2025-04-22
US20250188442A1 (en) 2025-06-12
CN114667344A (zh) 2022-06-24
BR112022004453A2 (pt) 2022-06-21
EP4028512A4 (en) 2023-09-20

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