CN114659935A - Method and device for measuring viscosity of trace liquid and flow resistance of micro-channel - Google Patents
Method and device for measuring viscosity of trace liquid and flow resistance of micro-channel Download PDFInfo
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- CN114659935A CN114659935A CN202210271293.1A CN202210271293A CN114659935A CN 114659935 A CN114659935 A CN 114659935A CN 202210271293 A CN202210271293 A CN 202210271293A CN 114659935 A CN114659935 A CN 114659935A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N11/00—Investigating flow properties of materials, e.g. viscosity, plasticity; Analysing materials by determining flow properties
- G01N11/02—Investigating flow properties of materials, e.g. viscosity, plasticity; Analysing materials by determining flow properties by measuring flow of the material
- G01N11/04—Investigating flow properties of materials, e.g. viscosity, plasticity; Analysing materials by determining flow properties by measuring flow of the material through a restricted passage, e.g. tube, aperture
- G01N11/08—Investigating flow properties of materials, e.g. viscosity, plasticity; Analysing materials by determining flow properties by measuring flow of the material through a restricted passage, e.g. tube, aperture by measuring pressure required to produce a known flow
Abstract
The invention relates to a device for measuring viscosity of trace liquid and flow resistance of a micro-channel, which comprises a handheld portable measuring instrument internally integrated with a differential pressure sensor, a control chip, a power switch, two micro-pipeline connecting probes and an LCD/LED screen, wherein the differential pressure sensor is used for acquiring the pressure difference of liquid flowing in the micro-channel in real time; the control chip is used for reading, calculating and outputting data; the two micro-pipeline connecting probes are used for being inserted into any position of a flow channel on the micro-fluidic chip to be measured so as to measure the pressure difference of liquid flowing between the two points; the LCD/LED screen is used for user control and display of the measurement results. The invention has the following advantages: the method simplifies the operation flow and experimental equipment, can realize miniaturization and portability, and innovatively solves the problem of precision measurement of local flow resistance of any microfluidic chip with a silica gel flow channel.
Description
Technical Field
The invention relates to the technical field of liquid flow resistance and viscosity measurement, in particular to a method and a device for measuring the viscosity of trace liquid and the flow resistance of a micro-channel.
Background
The microfluidic chip is a scientific technology which is mainly characterized by controlling fluid in a micrometer-scale space, and has the capability of shrinking the basic functions of laboratories such as biology, chemistry and the like onto a chip with a few square centimeters, so the microfluidic chip is also called a lab-on-a-chip. The micro-fluidic technology has the advantages of micro-scale, accurate and controllable, real-time observation and the like, so that the micro-fluidic technology is more and more widely applied in the fields of biology, chemical engineering and the like, and many micro-fluidic applications require that fluid is accurately transmitted along a channel network with a complex mode. Therefore, it is very important to accurately characterize and measure the hydraulic resistance of each channel segment and accurately control the fluid flow in the chip. Flow resistance plays an important role in many applications, such as interface positioning, concentration distribution networks, droplet generation in oil media, generation of different shear forces, etc.
The method for measuring the flow resistance and the viscosity of the trace liquid in the prior art mainly has the following defects:
firstly, most of the existing methods are complex in operation, cannot be used for finished products of microfluidic chips, and cannot detect local flow resistance on the chips;
secondly, the capability of measuring the flow resistance of any local position of the microfluidic chip is not provided;
and thirdly, the viscosity of the trace liquid cannot be measured by using a traditional viscometer.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention provides a method and a device for measuring the viscosity of a micro liquid and the flow resistance of a micro channel, which solve the problems in the background art.
In order to achieve the purpose, the technical scheme provided by the invention is as follows: a device for measuring viscosity of trace liquid and flow resistance of a micro-channel comprises a handheld portable measuring instrument internally integrated with a differential pressure sensor, a control chip, a power switch, two micro-pipeline connecting probes and an LCD/LED screen, wherein the differential pressure sensor is used for acquiring pressure difference of liquid flowing in the micro-channel in real time; the control chip is used for reading, calculating and outputting data; the two micro-pipeline connecting probes are used for being inserted into any position of a flow channel on the micro-fluidic chip to be measured so as to measure the pressure difference of liquid flowing between the two points; the LCD/LED screen is used for user control and display of the measurement results.
As an improvement: one end of each micro-pipeline connecting probe is connected with a differential pressure sensor inside the measuring instrument, the other end of each micro-pipeline connecting probe is connected with a hard pipe of a specially-made metal pipe probe, and the hard pipe needs to be filled with insulating oil or any non-corrosive liquid according to the connection mode difference of different types of differential pressure sensors.
As an improvement: when the chip is bonded by soft silica gel and a hard material, a metal tube with a v-shaped through channel at the bottom end is used; when the chip is bonded by two layers of soft silica gel, a metal tube with a through hole in the transverse direction is used. The depth of the former is not required to be controlled deliberately when the former is inserted into the metal pipe, the communication between the micro-channel and the V-shaped channel can be realized by directly inserting the former into the hard material, and the depth of the latter is controlled when the latter is inserted into the metal pipe, so that the transverse micro-channel is just communicated with the transverse through hole at the lower end of the metal pipe.
As an improvement: a method for measuring the viscosity of a trace liquid and the flow resistance of a micro-channel by using a measuring device comprises the following steps:
step 1: measuring the flow resistance of any section of microchannel of the microfluidic chip: firstly, a precision injection pump is used for introducing liquid into an inlet of the micro-fluidic chip to be detected at a certain flow rate Q, so that the whole channel is filled with the liquid. Ensuring that the connecting probe of the measuring instrument is filled with insulating oil or other non-corrosive liquid, inserting the metal probe into two ends of any channel to be detected of the chip after the channel is filled with the liquid for detecting the pressure difference delta P generated when the liquid passes through the section of flow channel at a given flow speed, and obtaining the fluid resistance according to the Poisea law
And (3) adjusting the measuring instrument to a flow resistance measuring mode, inserting the two connecting probes into the position of the micro-channel to be measured, and inputting the known flow rate Q to obtain the flow resistance value of the micro-channel.
Step 2: precision measurement of viscosity of trace liquids: the measurement of the liquid viscosity is increased by a few steps on the basis of the step 1, and the fluid resistance can be defined as the fluid resistance due to the non-circular section of the micro-flow channel in the micro-flow control chip
In the formula, eta is the viscosity of the liquid, L is the length of the channel, and rhIs called hydraulic radius, and is defined as rh2A/P, wherein A is the cross-sectional area of the channel, and P is the perimeter of the cross-section of the channel, and the calculation formula of the viscosity of the liquid is obtained through further simplification
The method can realize the precise measurement of the viscosity of the trace liquid. When the viscosity of the trace liquid is measured, the measuring instrument is adjusted to a viscosity measuring mode, and the liquid with the viscosity to be measured is introduced into a standard viscosity measuring chip with known flow resistance and channel size parameters, so that the viscosity value of the trace liquid can be obtained.
As an improvement: the measuring device in the step 1 can be directly inserted into any position of the PDMS flow channel through a metal thin tube with an opening at the top end, so that the flow channel on the chip is connected with two inlets of the pressure sensor through the connecting probe, the liquid pressure is effectively transmitted to the pressure sensor, and the pressure difference delta P between any two positions of the microfluidic channel is measured in real time.
As an improvement: the flow resistance and the size parameter of the micro-channel of the standard viscosity measurement chip in the step 2 are known, and the standard viscosity measurement chip is matched with a measuring instrument for use, so that the viscosity measurement can be completed only by inputting the flow rate Q and without additional calibration.
After the technical scheme is adopted, the invention has the beneficial effects that: the method simplifies the operation flow and experimental equipment, can realize miniaturization and portability, and can accurately measure the flow resistance between any two points of the micro-fluidic chip micro-channel by inserting the connecting probe into the two points by a user without performing pretreatment on the existing micro-fluidic chip. The device innovatively solves the problem of precision measurement of the local flow resistance of the microfluidic chip, and the Poiseul formula is used for deduction calculation, so that the error is small, and the measurement is accurate. The viscosity measurement can be realized only by introducing a trace amount of liquid into a standardized viscosity measurement chip, so that the viscosity measurement of the liquid is more convenient and rapid, and the required liquid amount is less (a few microliters). Therefore, the method and the device have important application prospects in the fields of biology and microfluidics.
Drawings
FIG. 1 is a schematic view of the connection structure of a measuring apparatus for viscosity of a micro-liquid and flow resistance of a micro-channel according to the present invention;
FIG. 2 is a schematic view showing the external structure of a flow resistance and fluid viscosity measuring instrument of a measuring apparatus for viscosity of a micro-liquid and flow resistance of a micro-channel according to the present invention;
FIG. 3 is a schematic view showing the internal structure of a flow resistance and fluid viscosity measuring instrument of a measuring apparatus for viscosity of a micro-liquid and flow resistance of a micro-channel according to the present invention;
FIG. 4 is a schematic structural diagram of a flow resistance and fluid viscosity measuring instrument of a measuring device for micro-liquid viscosity and micro-channel flow resistance according to the present invention, which is a front view of a metal probe with an elliptical through hole for a double-layer soft silica gel chip;
FIG. 5 is a schematic diagram of a side view of a metal probe with an elliptical through hole for a double-layer soft silica gel chip of a flow resistance and fluid viscosity measuring instrument of a measuring device for micro-liquid viscosity and micro-channel flow resistance according to the present invention;
FIG. 6 is a schematic structural diagram of a flow resistance and fluid viscosity measuring apparatus of a measuring apparatus of micro-liquid viscosity and micro-channel flow resistance according to the present invention, which is used for a metal probe having a V-shaped through hole at the bottom end of a layer of soft silica gel chip and a layer of glass chip;
FIG. 7 is a schematic diagram of a side view of a metal probe of a flow resistance and fluid viscosity measuring apparatus for a device for measuring micro-fluid viscosity and micro-channel flow resistance according to the present invention, the metal probe having a V-shaped through hole at the bottom end of a layer of soft silica gel chip and a layer of glass chip;
FIG. 8 is a schematic view of a connection structure of a metal probe, a layer of soft silica gel chip and a layer of glass chip of the measuring device for measuring the viscosity of the micro-liquid and the flow resistance of the micro-channel according to the present invention;
FIG. 9 is a schematic diagram of the flow in the micro-channel after the double-layer soft silica gel chip of the measuring device for viscosity of micro-liquid and flow resistance of the micro-channel of the invention is connected with the probe;
FIG. 10 is a schematic view of the flow in the micro-channel after the double-layer soft silica gel chip of the measuring device for the viscosity of the micro-liquid and the flow resistance of the micro-channel is connected with the probe;
FIG. 11 is a schematic diagram of the flow in the micro-channel after a layer of soft silica gel chip and a layer of glass chip of the device for measuring the viscosity of the micro-liquid and the flow resistance of the micro-channel are connected with a probe according to the invention;
as shown in the figure: 1. flow resistance and fluid viscosity meters; 2. a metal probe; 3. a precision syringe pump; 4. a micro-fluidic chip to be tested; 5. a waste liquid outlet; 6. LCD/LED screens; 7. a power switch; 8. a pressure sensor; 9. a soft silica gel chip; 10. A microchannel; 11. a glass chip; 12. and a control chip.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings.
Example (b): as shown in fig. 1 to 6, a device for measuring viscosity of trace liquid and flow resistance of a microchannel comprises a handheld portable measuring instrument with a differential pressure sensor, a control chip, a power switch, two microchannel connection probes and an LCD/LED screen integrated inside, wherein the differential pressure sensor is used for acquiring pressure difference of liquid flowing in the microchannel in real time; the control chip is used for reading, calculating and outputting data; the two micro-pipeline connecting probes are used for being inserted into any position of a flow channel on the micro-fluidic chip to be measured so as to measure the pressure difference of liquid flowing between the two points; the LCD/LED screen is used for user control and display of the measurement results.
The two micro-pipeline connecting probes are hard pipes, one ends of the hard pipes are connected with differential pressure sensors in the measuring instrument, the other ends of the hard pipes are connected with special metal pipe probes, and according to the difference of the connection modes of the differential pressure sensors of different types, the hard pipes need to be soaked with insulating oil or any non-erosive liquid.
When the chip is bonded by soft silica gel and a hard material, a metal tube with a v-shaped through channel at the bottom end is used; when the chip is bonded by two layers of soft silica gel, a metal tube with a through hole in the transverse direction is used. The depth of the micro-channel is not required to be controlled deliberately when the micro-channel is inserted into the metal pipe, the micro-channel can be communicated with the V-shaped channel by directly inserting the micro-channel into a hard material, and the depth of the micro-channel is required to be controlled when the micro-channel is inserted into the metal pipe, so that the transverse micro-channel is just communicated with the transverse through hole at the lower end of the metal pipe.
A method for measuring the viscosity of a trace liquid and the flow resistance of a micro-channel by using a measuring device comprises the following steps:
step 1: measuring the flow resistance of any section of microchannel of the microfluidic chip: firstly, a precision injection pump is used for introducing liquid into an inlet of the micro-fluidic chip to be detected at a certain flow rate Q, so that the whole channel is filled with the liquid. Ensuring that the connecting probe of the measuring instrument is filled with insulating oil or other non-corrosive liquid, inserting the metal probe into two ends of any channel to be detected of the chip after the channel is filled with the liquid for detecting the pressure difference delta P generated when the liquid passes through the section of flow channel at a given flow speed, and obtaining the fluid resistance according to the Poisea law
And (3) adjusting the measuring instrument to a flow resistance measuring mode, inserting the two connecting probes into the position of the micro-channel to be measured, and inputting the known flow rate Q to obtain the flow resistance value of the micro-channel.
Step 2: precision measurement of viscosity of trace liquids: the measurement of the liquid viscosity is increased by a few steps on the basis of the step 1, and the fluid resistance can be defined as the fluid resistance due to the non-circular section of the micro-flow channel in the micro-flow control chip
In the formula, eta is the viscosity of the liquid, L is the length of the channel, and rhIs called hydraulic radius, and is defined as rh2A/P, wherein A is the cross-sectional area of the channel, and P is the perimeter of the cross-section of the channel, and the calculation formula of the viscosity of the liquid is obtained through further simplification
The method can realize the precise measurement of the viscosity of the trace liquid, when the viscosity of the trace liquid is measured, the measuring instrument is adjusted to a viscosity measuring mode, and the liquid with the viscosity to be measured is introduced into a standard viscosity measuring chip with known flow resistance and channel size parameters, so that the viscosity value of the trace liquid can be obtained.
The measuring device in the step 1 can be directly inserted into any position of the PDMS flow channel through a metal thin tube with an opening at the top end, so that the flow channel on the chip is connected with two inlets of the pressure sensor through the connecting probe, the liquid pressure is effectively transmitted to the pressure sensor, and the pressure difference delta P between any two positions of the microfluidic channel is measured in real time.
The flow resistance and the size parameter of the micro-channel of the standard viscosity measurement chip in the step 2 are known, and the standard viscosity measurement chip is matched with a measuring instrument for use, so that the viscosity measurement can be completed only by inputting the flow rate Q and without additional calibration.
The present invention and its embodiments have been described above, and the description is not intended to be limiting, and the drawings are only one embodiment of the present invention, and the actual structure is not limited thereto. In summary, those skilled in the art should appreciate that they can readily use the disclosed conception and specific embodiments as a basis for designing or modifying other structures for carrying out the same purposes of the present invention without departing from the spirit and scope of the invention as defined by the appended claims.
Claims (6)
1. The utility model provides a measuring device of micro-liquid viscosity and microchannel flow resistance which characterized in that: the portable handheld measuring instrument comprises a differential pressure sensor, a control chip, a power switch, two micro-pipeline connecting probes and an LCD/LED screen, wherein the differential pressure sensor is integrated inside the portable handheld measuring instrument and is used for acquiring the pressure difference of liquid flowing in a micro-channel in real time; the control chip is used for reading, calculating and outputting data; the two micro-pipeline connecting probes are used for being inserted into any position of a flow channel on the micro-fluidic chip to be measured so as to measure the pressure difference of liquid flowing between the two points; the LCD/LED screen is used for user control and display of the measurement results.
2. The apparatus for measuring viscosity of trace liquid and flow resistance of microchannel according to claim 1, wherein: the two micro-pipeline connecting probes are hard pipes, one ends of the hard pipes are connected with differential pressure sensors in the measuring instrument, the other ends of the hard pipes are connected with special metal pipe probes, and according to the difference of the connection modes of the differential pressure sensors of different types, the hard pipes need to be soaked with insulating oil or any non-corrosive liquid.
3. The apparatus for measuring viscosity of trace liquid and flow resistance of microchannel according to claim 1, wherein: when the chip is bonded by soft silica gel and a hard material, a metal tube with a v-shaped through channel at the bottom end is used; when the chip is bonded by two layers of soft silica gel, a metal tube with a through hole in the transverse direction is used. The depth of the former is not required to be controlled deliberately when the former is inserted into the metal pipe, the communication between the micro-channel and the V-shaped channel can be realized by directly inserting the former into the hard material, and the depth of the latter is controlled when the latter is inserted into the metal pipe, so that the transverse micro-channel is just communicated with the transverse through hole at the lower end of the metal pipe.
4. The method for measuring the viscosity of a micro liquid and the flow resistance of a micro channel as claimed in claim 1, wherein: the method comprises the following steps:
step 1: measuring the flow resistance of any section of microchannel of the microfluidic chip: firstly, a precision injection pump is used for introducing liquid into an inlet of the micro-fluidic chip to be detected at a certain flow rate Q, so that the whole channel is filled with the liquid. Ensuring that the connecting probe of the measuring instrument is filled with insulating oil or other non-corrosive liquid, inserting the metal probe into two ends of any channel to be detected of the chip after the channel is filled with the liquid, detecting the pressure difference delta P generated when the liquid passes through the section of flow channel at a given flow speed, and obtaining the fluid resistance according to the Poisea law
And (3) adjusting the measuring instrument to a flow resistance measuring mode, inserting the two connecting probes into the position of the micro-channel to be measured, and inputting the known flow rate Q to obtain the flow resistance value of the micro-channel.
Step 2: precision measurement of viscosity of trace liquids: the measurement of the liquid viscosity is increased by a few steps on the basis of the step 1, and the fluid resistance can be defined as the fluid resistance due to the non-circular section of the micro-flow channel in the micro-flow control chip
In the formula, eta is the viscosity of the liquid, L is the length of the channel, and rhIs called hydraulic radius, and is defined as rh2A/P, wherein A is the cross-sectional area of the channel, and P is the perimeter of the cross-sectional area of the channel, and the calculation formula of the viscosity of the liquid is obtained by further simplification
The method can realize the precise measurement of the viscosity of the trace liquid. When the viscosity of the trace liquid is measured, the measuring instrument is adjusted to a viscosity measuring mode, and the liquid with the viscosity to be measured is introduced into a standard viscosity measuring chip with known flow resistance and channel size parameters, so that the viscosity value of the trace liquid can be obtained.
5. The method for measuring the viscosity of a micro liquid and the flow resistance of a micro channel as claimed in claim 4, wherein: the measuring device in the step 1 can be directly inserted into any position of the PDMS flow channel through a metal thin tube with an opening at the top end, so that the flow channel on the chip is connected with two inlets of the pressure sensor through the connecting probe, the liquid pressure is effectively transmitted to the pressure sensor, and the pressure difference delta P between any two positions of the microfluidic channel is measured in real time.
6. The method for measuring the viscosity of a micro liquid and the flow resistance of a micro channel as claimed in claim 4, wherein: the flow resistance and the size parameter of the micro-channel of the standard viscosity measurement chip in the step 2 are known, and the standard viscosity measurement chip is matched with a measuring instrument for use, so that the viscosity measurement can be completed only by inputting the flow rate Q and without additional calibration.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202210271293.1A CN114659935A (en) | 2022-03-18 | 2022-03-18 | Method and device for measuring viscosity of trace liquid and flow resistance of micro-channel |
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| CN202210271293.1A CN114659935A (en) | 2022-03-18 | 2022-03-18 | Method and device for measuring viscosity of trace liquid and flow resistance of micro-channel |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115436228A (en) * | 2022-10-11 | 2022-12-06 | 山东大学 | System and method for measuring viscosity of micro-upgrading liquid sample |
| CN115436227A (en) * | 2022-10-11 | 2022-12-06 | 山东大学 | Micro-fluidic chip and method suitable for measuring viscosity of micro-upgrading liquid sample |
| CN116237103A (en) * | 2023-05-11 | 2023-06-09 | 杭州博日科技股份有限公司 | Microfluidic chip |
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2022
- 2022-03-18 CN CN202210271293.1A patent/CN114659935A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115436228A (en) * | 2022-10-11 | 2022-12-06 | 山东大学 | System and method for measuring viscosity of micro-upgrading liquid sample |
| CN115436227A (en) * | 2022-10-11 | 2022-12-06 | 山东大学 | Micro-fluidic chip and method suitable for measuring viscosity of micro-upgrading liquid sample |
| CN116237103A (en) * | 2023-05-11 | 2023-06-09 | 杭州博日科技股份有限公司 | Microfluidic chip |
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