CN114651896A

CN114651896A - Feed additive and preparation facilities of clean feed quality

Info

Publication number: CN114651896A
Application number: CN202210320324.8A
Authority: CN
Inventors: 赵晓南; 胡友军; 刘坚; 程皇座; 宾艳芳; 孙丹丹; 胡文继
Original assignee: Guangdong Acid Power Biotechnology Co ltd
Current assignee: Guangdong Acid Power Biotechnology Co ltd
Priority date: 2022-03-29
Filing date: 2022-03-29
Publication date: 2022-06-24

Abstract

The invention provides a feed additive with clean feed quality and a preparation device thereof, wherein the feed additive comprises the following raw materials in percentage by content: 1-10% of caproic acid, 10-30% of caprylic acid, 10-25% of capric acid, 1-10% of lauric acid, 1-10% of monoglycerol laurate, 1-10% of caprylic/capric glyceride and 20-50% of silicon dioxide. The invention can effectively reduce the virus load in the feed and feed raw materials and inhibit the transmission of viruses in the feed on one hand, and can reduce the additive amount on the other hand, reduce the use cost and reduce the influence on the palatability of animals.

Description

Feed additive and preparation facilities of clean feed quality

Technical Field

The invention relates to the technical field of additives for feeds in the animal husbandry industry, in particular to a feed additive for cleaning feed quality and a preparation device.

Background

Common viral diseases still occur, and non-epidemic diseases usually appear to be due to earth rolling. The reason is that on one hand, new viruses are continuously discovered, known virus strains are continuously mutated, and legal and effective non-pestilence vaccines are not yet developed, so that the existing vaccines are slightly lagged in immunity and difficult to restrain viral infectious diseases, on the other hand, the vaccines are neglected in logistics transportation, and a farm carries out strict washing, disinfecting and drying procedures on livestock and poultry and feed transfer vehicles inside and outside the farm, but a plurality of researches show that after the feed and raw materials of the feed are polluted by the viruses, the viruses can survive in the feed for more than 20 days and are remotely transmitted to become a large vector for virus transmission. This phenomenon is also receiving more and more attention. In order to reduce the risk of feed infection, many feed enterprises granulate at high temperature of 85 ℃ for 3min, or process feed raw materials for 10-20 min at the temperature of more than 80 ℃.

However, high temperature treatment has certain effects, but also has limitations: on one hand, the nutrient components are destroyed to cause nutrient loss, on the other hand, the high-temperature sterilization is not always feasible, and the feed can be polluted again in the transportation and storage processes. Or adding substances with antiviral effect into feed, such as medium-chain fatty acid and monoglyceride thereof, but has certain disadvantages: on the one hand, the addition amount is large, and the use cost is high. 1-4% of dosage is commonly used at home and abroad as scientific experimental research; secondly, the ingestion is influenced, the medium-chain fatty acid has special mutton smell, and the animal palatability is influenced when the addition amount exceeds 5 percent; thirdly, the combination proportion of the medium-chain fatty acid and the monoglyceride thereof is not fixed and is added and combined randomly.

Disclosure of Invention

In order to overcome the above disadvantages of the prior art, the present invention provides a feed additive and a preparation device for clean feed quality, so as to solve the above technical problems.

The technical scheme adopted by the invention for solving the technical problems is as follows:

a feed additive for clean feed quality comprises the following raw materials in percentage by content: 1-10% of caproic acid, 10-30% of caprylic acid, 10-25% of capric acid, 1-10% of lauric acid, 1-10% of monoglyceride laurate, 1-10% of caprylic-capric glyceride and 20-50% of silicon dioxide.

As a further improvement of the invention: comprises the following raw materials in percentage by weight: 1-5% of caproic acid, 15-25% of caprylic acid, 15-20% of capric acid, 1-10% of lauric acid, 1-5% of glycerol monolaurate and 20-50% of silicon dioxide.

As a further improvement of the invention: comprises the following raw materials in percentage by weight: 2-5% of caproic acid, 20-25% of caprylic acid, 15-20% of capric acid, 1-5% of lauric acid, 1-5% of monoglyceride of lauric acid and 30-50% of silicon dioxide.

As a further improvement of the invention: comprises the following raw materials in percentage by weight: 5% caproic acid, 25% caprylic acid, 15% capric acid, 5% lauric acid, 5% monoglyceryl laurate and 45% silica.

As a further improvement of the invention: comprises the following raw materials in percentage by weight: 3% caproic acid, 18% caprylic acid, 20% capric acid, 4% lauric acid, 5% monoglyceryl laurate, 10% caprylic/capric glycerides and 40% silica.

As a further improvement of the invention: the caprylic capric glyceride is caprylic glyceride, capric glyceride, or caprylic capric glyceride mixture.

As a further improvement of the invention: the raw materials are obtained by screening through a computer molecular simulation technology.

A preparation device of a feed additive with clean feed quality is disclosed, wherein a plurality of acid agent storage mechanisms, a mixing reaction kettle, a plurality of ester agent storage mechanisms, a silicon dioxide storage mechanism and a spraying and mixing mechanism are arranged; the acid agent storage mechanism and the ester agent storage mechanism are respectively connected with the mixing reaction kettle, the mixing reaction kettle is connected with the spraying mechanism, the spraying mixing mechanism is connected with the silicon dioxide storage mechanism, and the mixing reaction kettle and the spraying mixing mechanism are respectively connected with the control center.

As a further improvement of the invention: the mixing reaction kettle comprises a stirring mechanism, a heating mechanism and a cooling mechanism; the stirring mechanism is arranged in the mixing reaction kettle, the heating mechanism is arranged below the mixing reaction kettle, and the cooling mechanism is arranged on one side of the mixing reaction kettle.

As a further improvement of the invention: the spraying and mixing mechanism comprises a spraying component, a mixing component, a heating component and a cooling component; the spraying assembly is arranged above the spraying and mixing mechanism, the mixing assembly is arranged in the spraying and mixing mechanism, the warming assembly is arranged below the spraying and mixing mechanism, and the cooling assembly is arranged on one side of the spraying and mixing mechanism.

Compared with the prior art, the invention has the beneficial effects that:

on one hand, the feed additive can effectively reduce the virus load in feed and feed raw materials and inhibit the spread of viruses in the feed, on the other hand, the additive amount can be reduced, the use cost can be reduced, and the influence on the palatability of animals is reduced.

Drawings

FIG. 1 is a schematic representation of the viral load in a feed sample of the invention.

FIG. 2 is a schematic view of the structure of a production apparatus of the present invention.

Detailed Description

The invention will now be further described with reference to the following description and examples in conjunction with the accompanying drawings:

the embodiment of the invention provides a feed additive with clean feed quality, which comprises the following raw materials in percentage by content: 1-10% of caproic acid, 10-30% of caprylic acid, 10-25% of capric acid, 1-10% of lauric acid, 1-10% of monoglyceride laurate, 1-10% of caprylic-capric glyceride and 20-50% of silicon dioxide.

In the present invention, by using the above-mentioned raw materials in combination, viruses can be efficiently inactivated. The combination of caproic acid, caprylic acid and capric acid has higher digestion, absorption and oxidation energy supply speeds in animal bodies, can effectively improve the growth and development of animals, and particularly can improve the small intestinal villus height of piglets and reduce the depth of crypts for piglets, thereby improving the intestinal function, effectively improving the digestion and absorption function of piglets and adjusting the intestinal flora, and simultaneously participating in the immune regulation of piglets through signal protein in immune cells. Capric acid and lauric acid have excellent antibacterial function in fatty acid and have immunoregulatory property. The lauric acid monoglyceride has antiviral activity, especially has obvious inhibiting effect on enveloped viruses, especially has good preventing and treating effect on porcine reproductive and respiratory syndrome, and the principle is that the replication of the porcine reproductive and respiratory syndrome is inhibited by destroying the lipid bilayer of the envelope of the porcine reproductive and respiratory syndrome. The caprylic capric glyceride has excellent emulsifying property and is a good emulsifier. The silica serves as a coating material for the feed additive.

In the second embodiment of the invention, the mixture ratio of the first embodiment is further improved, and the mixture ratio comprises the following raw materials in percentage by content: 1-5% of caproic acid, 15-25% of caprylic acid, 15-20% of capric acid, 1-10% of lauric acid, 1-5% of glycerol monolaurate and 20-50% of silicon dioxide.

In the third embodiment of the invention, the mixture ratio of the first embodiment is further improved, and the mixture ratio comprises the following raw materials in percentage by content: 2-5% of caproic acid, 20-25% of caprylic acid, 15-20% of capric acid, 1-5% of lauric acid, 1-5% of monoglyceride of lauric acid and 30-50% of silicon dioxide.

In the fourth embodiment of the invention, the mixture ratio of the first embodiment is further improved: comprises the following raw materials in percentage by weight: 5% caproic acid, 25% caprylic acid, 15% capric acid, 5% lauric acid, 5% monoglyceryl laurate and 45% silica.

In the fifth embodiment of the invention, through a plurality of tests, a better mixture ratio is obtained: comprises the following raw materials in percentage by weight: 3% caproic acid, 18% caprylic acid, 20% capric acid, 4% lauric acid, 5% monoglyceryl laurate, 10% caprylic/capric glycerides and 40% silica.

Preferably, the caprylic capric glyceride is caprylic glyceride, capric glyceride or a caprylic capric glyceride mixture.

As a further improvement of the invention: the raw materials are obtained by screening through a computer molecular simulation technology. The feed additive is prepared from raw materials which are screened by a computer molecular simulation technology and have strong binding capacity with a plurality of characteristic proteins of a plurality of viruses, and the high efficiency and stable performance of the feed additive are ensured.

The viruses include various viruses in animal industry, such as epidemic diarrhea, pseudorabies, blue ear, foot and mouth disease, transmissible gastroenteritis, African swine fever, and avian influenza.

In an embodiment of the present invention, there is further provided a device for preparing a feed additive of clean feed quality for preparing the feed additive, as shown in fig. 2, wherein a plurality of acid agent storage mechanisms 1, a mixing reaction kettle 2, a plurality of ester agent storage mechanisms 3, a silica storage mechanism 4 and a spraying and mixing mechanism 5; the acid agent storage mechanism 1 and the ester agent storage mechanism 3 are respectively connected with the mixed reaction kettle 2, the mixed reaction kettle 2 is connected with the spraying mechanism, the spraying and mixing mechanism 5 is connected with the silicon dioxide storage mechanism 4, and the mixed reaction kettle 2 and the spraying and mixing mechanism 5 are respectively connected with the control center. The acid agent storage means 1 includes caproic acid storage means, caprylic acid storage means, capric acid storage means, and lauric acid storage means. The ester agent storage mechanism 3 comprises a lauric acid monoglyceride storage mechanism and a caprylic capric acid glyceride storage mechanism.

The mixing reactor 2 of this example is described in further detail below: the mixing reaction kettle 2 comprises a stirring mechanism 21, a heating mechanism 22 and a cooling mechanism 23; stirring mechanism 21 sets up in mixing reation kettle 2, heating mechanism 22 sets up mixing reation kettle 2 below, cooling mechanism 23 sets up one side of mixing reation kettle 2. The heating mechanism 22 comprises a mixing electric heating net 221 arranged on the inner wall of the mixing reaction kettle 2 and a mixing electric heating controller 222 arranged at the bottom of the mixing reaction kettle 2. The hybrid electric heating net 221 is controlled by the hybrid electric heating controller 222 to heat, and the heating temperature and time are regulated and controlled by the control center. Cooling mechanism 23 is including setting up mixing cooling pipe network 231 in the cauldron wall of mixing reation kettle 2, and set up and be in the mixed cooling controller 232 of mixing reation kettle 2 one side, mix cooling pipe network 231 through mixed cooling controller 232 control and cool down, and the duration and the temperature of cooling are regulated and control through control center.

The spray mixing mechanism 5 of the present embodiment is described in further detail below: the spraying and mixing mechanism 5 comprises a spraying assembly 51, a mixing assembly 52, a heating assembly 53 and a cooling assembly 54; the spraying assembly 51 is arranged above the spraying and mixing mechanism 5, the mixing assembly 52 is arranged in the spraying and mixing mechanism 5, the temperature rising assembly 53 is arranged below the spraying and mixing mechanism 5, and the temperature lowering assembly 54 is arranged on one side of the spraying and mixing mechanism 5. After the mixture in the mixing reaction kettle 2 is mixed twice, the mixture is transported into a spraying assembly 51 through a transporting pipe 6, the spraying assembly 51 comprises a plurality of spraying openings 511 arranged around the mixing assembly 52, and the mixture is sprayed through the plurality of spraying openings 511. The temperature raising component 53 comprises an electric temperature raising net 531 arranged on the inner wall of the spraying and mixing mechanism 5 and a spraying temperature raising controller 532 arranged at the bottom of the spraying and mixing mechanism 5. The temperature of the electric heating net 531 is controlled by the spraying heating controller 532, and the temperature and the time are regulated and controlled by the control center. The cooling component 54 comprises a spraying cooling pipe network 541 arranged in the inner wall of the spraying mixing mechanism 5 and a spraying cooling controller 542 arranged on one side of the spraying mixing mechanism 5; the spraying and cooling pipe network 541 is controlled by the spraying and cooling controller 542 to cool, wherein the cooling time and temperature are regulated and controlled by the control center.

Each storage mechanism is used for storing raw materials, when the raw materials are prepared, caproic acid, caprylic acid, capric acid and lauric acid are added into the mixing reaction kettle 2 for first mixing and stirring, wherein the stirring speed and the stirring time of the stirring mechanism 21 are controlled by the control center, the stirring temperature is controlled by the control center through controlling the heating mechanism 22 and the cooling mechanism 23, after the first stirring is completed, ester agents required in the mixture ratio are added, the specific types of the ester agents are set according to different mixture ratios, after the ester agents are added, second mixing and stirring are carried out, esterification treatment is carried out, and the same period is carried out, and the stirring speed, the stirring time and the stirring temperature are controlled by the control center. After the esterification is finished, firstly placing the silicon dioxide in the spraying and mixing mechanism 5, then spraying the esterified mixture onto the silicon dioxide through the spraying component 51, controlling the temperature during spraying through the control center to control the temperature rising component 53 and the temperature lowering component 54, after the spraying is finished, stirring through the stirring component, cooling through the control center to control the temperature lowering component 54 after stirring, and cooling to the room temperature to obtain the feed additive.

The experimental method of the invention comprises the following steps:

1. taking 1ml of the stock solution of the frozen and stored PEDV virus, diluting the stock solution to 10ml by using PBS, uniformly mixing, and freezing for later use.

2. Taking 250g of complete feed for weaned piglets in the growth stage, averagely dividing into 5 parts, each 50.00g, placing the feed into a feed bottle, adding 0, 0.25, 0.5, 1.0 and 2.0g of additive samples into each kilogram of feed, mixing, sealing, marking 0, 0.25, 0.5, 1.0 and 2.0 on the bottle body according to the additive amount of the additive, and storing in a normal feed storage environment in a warehouse. The treatments are shown in table 1:

table 1 is a feed grouping table, as follows:

grouping	Viral fluid addition (ml)	Additive addition (g/kg)
			0	2.0	0
0.25	2.0	0.25
			0.5	2.0	0.50
1.0	2.0	1.00
			2.0	2.0	2.00

3. Immediately after the feed has been treated, it is mixed and 1g of each feed sample, designated 0d, is weighed out of each group and then sampled every 24h for a total of 8 samples. After the sample is obtained, 1.5ml of PBS is added, the mixture is rapidly and violently mixed, the mixture is stood on ice for 10min and then centrifuged at 12000rpm for 5min, 300 mu l of supernatant is taken, total RNA is extracted by a Trizol method and then is reversely transcribed into cDNA, and then the virus load in the sample is detected by qPCR. The reaction system is shown in the table 2, and the reaction procedure is as follows: pre-denaturation at 95 ℃ for 30 s; denaturation at 95 ℃ for 10 s; the fire is extinguished for 30s at 58 degrees; 40 cycles were performed. Dissolution curve: 95 ℃ and 65 ℃ at a rise of 0.5 ℃ to 95 ℃ per second.

Table 2 shows the fluorescent quantitative reaction system as follows:

primer F	0.5μL
		Primer R	0.5μL
ddH2O	3μL
		SYBR Green Premix Ex TaqⅡ	5μL
Form panel	1μL
		Total	10μL

4. The experimental results are as follows:

as can be seen from figure 1, the additive has a significant reduction effect on the viral load in PEDV contaminated feed. When the maximum dose is 2g/kg, compared with a control group, the virus load in the feed can be reduced by 10% on day 0; when the minimum dose of 0.25g/kg is added, compared with a control group, the virus load in the feed is reduced by 8% on the 1 st day, 18% on the 2 nd day and 26% on the 3 rd day. The additive is shown to be used in low dosage and to be highly effective in reducing the PEDV load in feed.

The above description is only an example of the present invention and should not be taken as limiting the scope of the invention, which is intended to cover any modifications, equivalents, improvements, etc. within the spirit and scope of the present invention.

The main functions of the invention are as follows: is applied to feed additives of various livestock and poultry, in particular to feed additives aiming at clean feed quality.

In summary, after reading the present disclosure, those skilled in the art can make various other corresponding changes without creative mental labor according to the technical solutions and concepts of the present disclosure, and all of them are within the protection scope of the present disclosure.

Claims

1. The feed additive for cleaning feed quality is characterized by comprising the following raw materials in percentage by content: 1-10% of caproic acid, 10-30% of caprylic acid, 10-25% of capric acid, 1-10% of lauric acid, 1-10% of monoglyceride laurate, 1-10% of caprylic-capric glyceride and 20-50% of silicon dioxide.

2. The clean feed quality feed additive as claimed in claim 1, which comprises the following raw materials in percentage by weight: 1-5% of caproic acid, 15-25% of caprylic acid, 15-20% of capric acid, 1-10% of lauric acid, 1-5% of monoglyceride of lauric acid and 20-50% of silicon dioxide.

3. The clean feed quality feed additive as claimed in claim 1, which comprises the following raw materials in percentage by weight: 2-5% of caproic acid, 20-25% of caprylic acid, 15-20% of capric acid, 1-5% of lauric acid, 1-5% of monoglyceride of lauric acid and 30-50% of silicon dioxide.

4. The clean feed quality feed additive as claimed in claim 1, which comprises the following raw materials in percentage by weight: 5% caproic acid, 25% caprylic acid, 15% capric acid, 5% lauric acid, 5% monoglyceryl laurate and 45% silica.

5. The clean feed quality feed additive as claimed in claim 1, which comprises the following raw materials in percentage by weight: 3% caproic acid, 18% caprylic acid, 20% capric acid, 4% lauric acid, 5% monoglyceryl laurate, 10% caprylic/capric glycerides and 40% silica.

6. The clean feed quality feed additive as claimed in claims 1 to 5, wherein the caprylic/capric glyceride is caprylin, capric glyceride, or a mixture of caprylic/capric glycerides.

7. A clean feed quality feed additive as claimed in any one of claims 1 to 5, wherein the raw material is selected by computer molecular simulation.

8. A preparation device of a feed additive with clean feed quality is characterized in that a plurality of acid agent storage mechanisms, a mixing reaction kettle, a plurality of ester agent storage mechanisms, a silicon dioxide storage mechanism and a spraying and mixing mechanism are arranged; the acid agent storage mechanism and the ester agent storage mechanism are respectively connected with the mixing reaction kettle, the mixing reaction kettle is connected with the spraying mechanism, the spraying mixing mechanism is connected with the silicon dioxide storage mechanism, and the mixing reaction kettle and the spraying mixing mechanism are respectively connected with the control center.

9. The clean feed quality feed additive of claim 8, wherein the mixing reactor comprises a stirring mechanism, a heating mechanism and a cooling mechanism; the stirring mechanism is arranged in the mixing reaction kettle, the heating mechanism is arranged below the mixing reaction kettle, and the cooling mechanism is arranged on one side of the mixing reaction kettle.

10. The clean feed quality feed additive of claim 8, wherein the spray mixing mechanism comprises a spray assembly, a mixing assembly, a temperature raising assembly and a temperature lowering assembly; the spraying assembly is arranged above the spraying and mixing mechanism, the mixing assembly is arranged in the spraying and mixing mechanism, the warming assembly is arranged below the spraying and mixing mechanism, and the cooling assembly is arranged on one side of the spraying and mixing mechanism.