CN114651725A

CN114651725A - Orchid seedling group banks up device with earth based on intelligent regulation and control

Info

Publication number: CN114651725A
Application number: CN202210422981.3A
Authority: CN
Inventors: 蒋彧; 何俊蓉; 刘雅琴; 涂勋良
Original assignee: Horticulture Research Institute of Sichuan Academy of Agricultural Sciences
Current assignee: Horticulture Research Institute of Sichuan Academy of Agricultural Sciences
Priority date: 2022-04-21
Filing date: 2022-04-21
Publication date: 2022-06-24
Anticipated expiration: 2042-04-21
Also published as: CN114651725B

Abstract

The invention discloses an orchid seedling tissue culture device based on intelligent regulation and control, and relates to the technical field of orchid seedling tissue culture devices.

Description

Orchid seedling group banks up device with earth based on intelligent regulation and control

Technical Field

The invention relates to the technical field of orchid seedling tissue culture devices, in particular to an orchid seedling tissue culture device based on intelligent regulation and control.

Background

The tissue culture propagation of orchid seedlings is a process of obtaining a certain part of tissue of an orchid and carrying out subculture in an isolated state by adopting a tissue culture mode to generate a new plant, wherein the certain part of the tissue of the orchid is usually part of a root, a stem and a branch or leaves, but the traditional device has some defects, cannot adapt to different nutritional requirements of various tissues of the orchid, causes a narrow application range, and cannot continuously and efficiently generate the orchid seedlings with high quality by different tissues of the orchid in a tissue culture mode;

in view of the above technical drawbacks, a solution is proposed.

Disclosure of Invention

The invention aims to: on the basis of generating orchid seedlings through continuous tissue culture, efficient and high-quality tissue culture of the orchid seedlings is achieved, and micro-pressure control of micro-addition liquid, addition of the micro-addition liquid in quantitative control and selective control of the micro-addition liquid with different requirements and formulas are achieved through cooperation of the constant-pressure control assembly, the quantitative addition assembly and the switching driving assembly, so that the tissue culture device can be widely adapted to different nutritional requirements of tissue culture of various orchids, and the tissue culture range of the tissue culture device is wider;

in order to achieve the purpose, the invention adopts the following technical scheme:

the orchid seedling tissue culture device based on intelligent regulation comprises a tissue culture incubator, wherein a suspension centrifugal box and a tissue culture installation box are fixedly arranged in the tissue culture incubator, the suspension centrifugal box is arranged on the bottom wall of the tissue culture incubator, a centrifugal pushing assembly is arranged on the suspension centrifugal box, the centrifugal pushing assembly is arranged on the tissue culture incubator, a plurality of tissue culture installation boxes are arranged at equal intervals, a tissue culture screen plate is movably clamped on the top surface of the tissue culture installation box, a plurality of tissue placement openings used for placing orchid tissues are formed in the tissue culture screen plate, a seepage screen used for supporting the orchid tissues is arranged at the bottom end of each tissue placement opening, and the seepage screen is fixedly arranged at the bottom end of each tissue culture screen plate;

the top of the suspension centrifugal box is connected with a distribution liquid pump through a pipeline in a through way, a liquid inlet of the distribution liquid pump is connected to the top of the suspension centrifugal box in a through way, a liquid outlet of the distribution liquid pump is connected with a distribution pipe through way, one end of the distribution pipe, far away from the distribution liquid pump, is connected to the center of the bottom end of the tissue culture installation box through way, two sides of the bottom of the suspension centrifugal box are connected with a flow guide pipe and a liquid inlet pipe through way, a liquid inlet throttle valve is installed on the liquid inlet pipe, one end of the flow guide pipe is connected with a flow guide hopper through way, the top end of the flow guide hopper is fixedly connected with a limit box, the limit box is connected with the flow guide hopper through way, the limit box is provided with a micro-adding control structure and a micro-material storage pipe group, the micro-adding control structure comprises a power box, a constant-pressure control component, a quantitative adding component and a switching drive component, the power box is fixedly arranged at the top end of the limit box, the micro-material storage tube group is embedded in the limiting box and movably connected with the limiting box, the micro-material storage tube group is provided with a plurality of micro-material storage tube groups, the micro-material storage tube groups are distributed by taking the circle center of the limiting box as the center and according to an annular array, the micro-material storage tube groups and the quantitative adding assembly are matched and arranged, the constant-pressure control assembly is abutted to the micro-material storage tube groups, and the constant-pressure control assembly and the quantitative adding assembly are connected with the switching driving assembly in a movable transmission mode.

Further, centrifugation promotes the subassembly by centrifugal motor, centrifugation bull stick, centrifugation cover, centrifugal flabellum, promotion cover and catch bar and constitutes, the fixed bottom of locating the group's culture of temperature constant box of centrifugal motor, the centrifugation bull stick rotates and locates the suspension centrifugation incasement, and the one end of centrifugation bull stick runs through the inner wall of suspension centrifugation case and the inner wall of group's culture of temperature constant box in proper order and extends to its outside and with the output shaft fixed connection of centrifugal motor, the outer end of centrifugation bull stick is equipped with centrifugation cover and promotion cover, and the fixed cover in outer end of centrifugation cover is equipped with centrifugal flabellum, the bottom of centrifugal flabellum is located to centrifugal flabellum, the fixed cover in outer end of promotion cover is equipped with the catch bar, the top of centrifugal flabellum is located to the catch bar.

Further, the micro-material storage tube group comprises a liquid storage tube, a sliding block, a telescopic rod and an extrusion block, the sliding block is arranged in the liquid storage tube in a sliding mode, the top end of the sliding block is fixedly connected with the telescopic rod, the top end, far away from the sliding block, of the telescopic rod penetrates through the top wall of the liquid storage tube in a sliding mode, extends to the outside of the top wall of the liquid storage tube and is fixedly connected with the extrusion block, and the bottom end face of the liquid storage tube is far away from the center and is provided with a liquid outlet.

Furthermore, the constant pressure control assembly comprises a lifting panel, the lifting panel is provided with a plurality of clamping grooves, the clamping grooves are formed in the outer ring side of the bottom of the lifting panel, the clamping grooves are distributed in an annular array by taking the circle center of the lifting panel as the center, the extrusion blocks are movably clamped in the clamping grooves, the lifting panel is sleeved with a first limiting slide rod and a plurality of threaded rotating rods, the first limiting slide rod is in sliding connection with the lifting panel, the first limiting slide rods are distributed in an annular array by taking the circle center of the lifting panel as the center, the bottom ends of the first limiting slide rods are fixedly connected with the top end face of the limiting box, the threaded rotating rods are in threaded connection with the lifting panel, the top ends of the threaded rotating rods and the first limiting slide rods are respectively provided with an anti-falling block, the bottom end face of the anti-falling block is movably abutted to the lifting panel, and the bottom ends of the threaded rotating rods are rotated to penetrate through the outer wall of the limiting box and extend into the inner part of the limiting box and are fixedly sleeved with a first bevel gear, the first bevel gear is movably meshed with the switching driving component.

Furthermore, the quantitative adding assembly comprises a second bevel gear, the second bevel gear is movably engaged with the switching driving assembly, the second bevel gear is fixedly connected with a first rotating rod, the first rotating rod is rotatably arranged, one end of the first rotating rod, which is far away from the second bevel gear, sequentially penetrates through the inner wall of the power box and the outer wall of the limiting box, extends into the limiting box, and is fixedly sleeved with a first connecting sleeve rod, one end of the first connecting sleeve rod, which is far away from the first rotating rod, is provided with a micro-control motor, an output shaft of the micro-control motor is fixedly sleeved with a driving gear, the driving gear is movably engaged with a quantitative gear, the quantitative gears are provided with a plurality of quantitative gears, the quantitative gears are distributed according to an annular array by taking the central axis of the hollow rotating rod as the circle center, the quantitative gears are arranged in one-to-one correspondence with the micro-material storage pipe groups, the quantitative gears are rotatably sleeved with the hollow rotating rod, the hollow rotating rod is fixedly arranged on the bottom wall of the limiting box, the utility model discloses a spacing bull stick, including spacing bull stick, fixed arc cover, L shape leak hole and liquid outlet activity through connection, the both ends butt of ration gear has spacing sealed wheel, the outer end of hollow bull stick is located to spacing sealed wheel fixed cover, one of them the top of spacing sealed wheel is equipped with joint elastic sleeve, the bottom joint of joint elastic sleeve and stock solution pipe, the fixed arc cover of outer end fixedly connected with of spacing sealed wheel, the fixed arc cover is kept away from the one end of spacing sealed wheel and the inner wall fixed connection of spacing case, L shape leak hole has been seted up to the ration gear, L shape leak hole and liquid outlet activity through connection, the side stream mouth has been seted up with hollow bull stick to L shape leak hole, the side stream mouth passes through L shape leak hole and liquid outlet activity through connection.

Furthermore, the L-shaped leakage holes are arranged in a plurality of numbers, the L-shaped leakage holes are distributed according to a slip ring array by taking the circle center of the quantitative gear as the center, the aperture of the L-shaped leakage holes is enlarged in an equal ratio, the maximum aperture of the L-shaped leakage holes is equal to the aperture of the liquid outlet, and the apertures of the liquid outlet and the side flow port are arranged in an equal size.

Further, the switching driving assembly comprises a lifting box, the lifting box is slidably arranged in the power box, a lifting air rod, a second connecting sleeve rod and a return spring are arranged on the lifting box, the lifting air rod is mounted on the bottom wall of the power box, one end of the second connecting sleeve rod is fixedly connected with the power box, the other end of the second connecting sleeve rod is sleeved with a second limiting slide rod which is fixedly arranged in the power box, the return spring is fixedly arranged at the bottom end of the power box, the other end of the return spring is fixedly arranged on the bottom wall of the power box, a servo motor is fixedly arranged in the power box, an output shaft of the servo motor is fixedly connected with a second rotating rod which is rotatably arranged in the lifting box, one end of the second rotating rod penetrates through the inner wall of the lifting box and extends to the outside of the lifting box and is fixedly sleeved with a third bevel gear, and the first bevel gear and the second bevel gear are oppositely arranged, and the first bevel gear and the second bevel gear and

the third bevel gears are respectively engaged with the movable gears.

Further, the base is installed to the bottom of group's thermostated container, a plurality of articulated arc doors are installed to the outer wall of group's thermostated container, every group banks up the installation box department with earth and all corresponds and set up an articulated arc door, install the door handle on the articulated arc door, group's group banks up the control panel of installing of thermostated container with earth, last start button and the display screen of installing of control panel.

In summary, due to the adoption of the technical scheme, the invention has the beneficial effects that:

the tissue culture device realizes the high-efficiency and high-quality tissue culture of the orchid seedlings on the basis of the continuous tissue culture of the orchid seedlings, and realizes the micro-pressure control of the micro-addition liquid, the quantitative control of the addition of the micro-addition liquid and the selection control of the micro-addition liquid with different requirements and formulas through the cooperation of the constant-pressure control component, the quantitative addition component and the switching drive component, so that the tissue culture device can be widely adapted to different nutritional requirements of various tissues of the orchid, the tissue culture adaptation range of the tissue culture device is wider, the problems that the traditional device cannot be adapted to different nutritional requirements of various tissues of the orchid, the application range is narrow, and the orchid seedlings cannot be continuously generated with high efficiency and high quality through different tissues of the orchid in a tissue culture mode are solved.

Drawings

FIG. 1 shows a perspective view of the present invention;

FIG. 2 shows a schematic of the structure of the present invention;

FIG. 3 shows a schematic of the construction of the centrifugal push assembly;

FIG. 4 shows a top view of a tissue culture mesh plate;

FIG. 5 shows a schematic of the structure of a micro-addition control structure;

FIG. 6 shows a partial enlarged view at A of FIG. 5;

FIG. 7 shows a schematic of the structure at the dosing assembly;

fig. 8 shows a sectional view at the dosing gear;

illustration of the drawings: 1. tissue culture thermostat; 2. a suspension centrifuge box; 3. a centrifugal pushing assembly; 4. tissue culture installation box; 5. tissue culture net plate; 6. a flow guide hopper; 7. a limiting box; 8. a micro-addition control structure; 9. a micro-material storage tube group; 10. a dispensing pump; 11. a distribution pipe; 12. a flow guide pipe; 13. a liquid inlet pipe; 14. a liquid inlet throttle valve; 101. a base; 102. a control panel; 103. a start button; 104. a hinged arc door; 105. a door handle; 201. a centrifugal motor; 202. centrifuging the rotating rod; 203. a centrifugal sleeve; 204. centrifuging the fan blades; 205. a push sleeve; 206. a push rod; 501. a tissue placement port; 502. a liquid-permeable net; 801. a power box; 802. a constant voltage control assembly; 803. a dosing assembly; 804. switching the driving assembly; 805. a lifting panel; 806. a first limit slide bar; 807. a threaded rotating rod; 808. a first bevel gear; 809. an anti-drop block; 810. a clamping groove; 811. a second bevel gear; 812. a first rotating lever; 813. a first connecting loop bar; 814. a micro-control motor; 815. a driving gear; 816. a dosing gear; 817. an L-shaped leak hole; 818. a limit sealing wheel; 819. clamping the elastic sleeve; 820. fixing the arc sleeve; 821. a hollow rotating rod; 822. lifting the air rod; 823. a lifting box; 824. a servo motor; 825. a second rotating rod; 826. a third bevel gear; 827. a second connecting loop bar; 828. a second limit slide bar; 829. a return spring; 901. a liquid storage pipe; 902. a slider; 903. a telescopic rod; 904. extruding the block; 905. and a liquid outlet.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example 1:

as shown in fig. 1-8, the orchid seedling tissue culture device based on intelligent regulation comprises a tissue culture incubator 1, wherein a base 101 is installed at the bottom of the tissue culture incubator 1, a plurality of hinged arc doors 104 are installed on the outer wall of the tissue culture incubator 1, a hinged arc door 104 is correspondingly arranged at each tissue culture installation box 4, a door handle 105 is installed on each hinged arc door 104, a control panel 102 is installed on the tissue culture incubator 1, and a starting button 103 and a display screen are installed on the control panel 102;

a suspension centrifugal box 2 and a tissue culture installation box 4 are fixedly arranged in the tissue culture incubator 1, the suspension centrifugal box 2 is arranged at the bottom wall of the tissue culture incubator 1, a centrifugal pushing assembly 3 is arranged on the suspension centrifugal box 2, the centrifugal pushing assemblies 3 are arranged on the tissue culture incubator 1, a plurality of tissue culture installation boxes 4 are arranged at equal intervals, a tissue culture screen plate 5 is movably clamped on the top surface of the tissue culture installation box 4, a plurality of tissue placement openings 501 for placing orchid tissues are formed in the tissue culture screen plate 5, a seepage net 502 for supporting the orchid tissues is arranged at the bottom end of each tissue placement opening 501, and the seepage net 502 is fixedly arranged at the bottom end of the tissue culture screen plate 5;

the top of the suspension centrifugal box 2 is connected with a distribution liquid pump 10 through a pipeline in a through way, a liquid inlet of the distribution liquid pump 10 is connected with the top of the suspension centrifugal box 2 through a through way, a liquid outlet 905 of the distribution liquid pump 10 is connected with a distribution pipe 11 through a through way, one end of the distribution pipe 11 away from the distribution liquid pump 10 is connected with the center of the bottom end of the tissue culture installation box 4 through a through way, two sides of the bottom of the suspension centrifugal box 2 are connected with a flow guide pipe 12 and a liquid inlet pipe 13 through a through way, a liquid inlet throttle valve 14 is installed on the liquid inlet pipe 13, one end of the flow guide pipe 12 is connected with a flow guide hopper 6 through a through way, the top end of the flow guide hopper 6 is fixedly connected with a limit box 7, and the limit box 7 is connected with the flow guide hopper 6 through a through way,

the limiting box 7 is provided with a micro-adding control structure 8 and a micro-material storage tube group 9, the micro-adding control structure 8 comprises a power box 801, a constant pressure control component 802, a quantitative adding component 803 and a switching driving component 804, the power box 801 is fixedly arranged at the top end of the limiting box 7, the micro-material storage tube group 9 is embedded in the limiting box 7 and movably connected with the limiting box, the micro-material storage tube group 9 is provided with a plurality of micro-material storage tube groups, the micro-material storage tube group 9 takes the circle center of the limit box 7 as the center and is distributed according to a ring array, different micro-additive liquid is stored in the micro-material storage tube group 9, the micro-material storage tube group 9 is detachably arranged and can be adapted to adjustment of more different culture liquids, the micro-material storage tube group 9 is adapted to the quantitative adding assembly 803, the constant pressure control assembly 802 is adapted to and abutted against the micro-material storage tube group 9, and the constant pressure control assembly 802 and the quantitative adding assembly 803 are movably connected with the switching driving assembly 804 in a transmission manner;

the control panel 102 starts a centrifugal motor 201 fixedly installed at the bottom end of the tissue culture incubator 1 to work and controls an output shaft of the centrifugal motor to rotate, the output shaft of the centrifugal motor 201 drives a centrifugal rotating rod 202 fixed with the centrifugal motor to rotate, the centrifugal rotating rod 202 simultaneously drives a centrifugal sleeve 203 and a pushing sleeve 205 fixedly sleeved with the centrifugal rotating rod to rotate, the centrifugal sleeve 203 and the pushing sleeve 205 rotate to respectively drive a centrifugal fan blade 204 and a pushing rod 206 fixed with the centrifugal motor to rotate, the pushing rods 206 are provided with a plurality of groups, the length of the pushing rods is reduced in an equal ratio, the centrifugal fan blade 204 centrifugally accelerates the liquid material in the tissue culture incubator 1 from the bottom to form a suspension, the pushing rod 206 with the length of the pushing rods is reduced in an equal ratio pushes the suspension from the centrifugal rotating rod 202 to the periphery, and the absorption of the distribution liquid pump 10 is facilitated; wherein the centrifugal motor 201, the centrifugal rotating rod 202, the centrifugal sleeve 203, the centrifugal fan blade 204, the pushing sleeve 205 and the pushing rod 206 form a centrifugal pushing assembly 3;

the micro-material storage tube group 9 is composed of a liquid storage tube 901, a slider 902, an expansion rod 903 and an extrusion block 904, wherein the slider 902 is arranged in the liquid storage tube 901 in a sliding manner, the top end of the slider 902 is fixedly connected with the expansion rod 903, the top end of the expansion rod 903, which is far away from the slider 902, penetrates through the top wall of the liquid storage tube 901 in a sliding manner and extends to the outside of the liquid storage tube and is fixedly connected with the extrusion block 904, a liquid outlet 905 is formed in the bottom end face of the liquid storage tube 901, which is far away from the center, the extrusion block 904 is extruded downwards, the expansion rod 903 moves into the liquid storage tube 901 after the extrusion block 904 is extruded, the expansion rod 903 moves into the liquid storage tube 901 and drives the slider 902 fixed with the expansion rod to slide downwards along the inner wall of the liquid storage tube 901, the slider 902 extrudes the micro additive liquid in the liquid storage tube 901 after sliding downwards, when the liquid outlet 905 is blocked, the micro additive liquid is pressurized, and when the liquid outlet 905 is communicated, the liquid is pressurized to more easily circulated;

the control panel 102 starts the servo motor 824 arranged in the lifting box 823 to work and controls the output shaft thereof to rotate, the output shaft of the servo motor 824 rotates to drive the second rotating rod 825 fixed with the servo motor to rotate, the second rotating rod 825 rotates to drive the third bevel gear 826 fixedly sleeved with the second rotating rod to rotate, and the third bevel gear 826 is arranged between the first bevel gear 808 and the second bevel gear 811, therefore, when the control panel 102 controls the lifting air rod 822 to push the power box 801 to move downwards, the lifting air rod 822 pushes the power box 801 to move downwards to make the third bevel gear 826 mesh with the second bevel gear 811, so that the rotating power of the third bevel gear 826 is transmitted to the second bevel gear 811, the second bevel gear 811 rotates in one direction, the quantitative adding component 803 works and is positioned at different micro-material storage tube groups 9, meanwhile, the power box 801 moves downwards to squeeze the return spring 829 to contract, when the power is transmitted to the second bevel gear 811, the lifting air lever 822 stops being controlled, at this time, the third bevel gear 826 is meshed with the first bevel gear 808 under the action of the return spring 829, and then the rotation direction of the output shaft of the servo motor 824 is controlled, so that the first bevel gear 808 is controlled to rotate in the forward direction or the reverse direction, and the constant pressure control assembly 802 works; wherein the lifting air rod 822, the lifting box 823, the servo motor 824, the second rotating rod 825, the third bevel gear 826, the second connecting loop bar 827, the second limit sliding rod 828 and the return spring 829 form the switching driving assembly 804;

the constant pressure control assembly 802 comprises a lifting panel 805, the lifting panel 805 is provided with a plurality of clamping grooves 810, the clamping grooves 810 are arranged on the outer ring side of the bottom of the lifting panel 805, the clamping grooves 810 are distributed according to an annular array by taking the circle center of the lifting panel 805 as the center, the extrusion blocks 904 are movably clamped in the clamping grooves 810, the lifting panel 805 is sleeved with a first limiting slide bar 806 and a threaded rotating bar 807, the first limiting slide bar 806 is in sliding connection with the lifting panel 805, the first limiting slide bars 806 are provided with a plurality of limiting slide bars 806, the first limiting slide bars 806 are distributed according to the annular array by taking the circle center of the lifting panel 805 as the center, the bottom end of the first limiting slide bar 806 is fixedly connected with the top end face of the limiting box 7, the threaded rotating bar 807 is in threaded connection with the lifting panel 805, the top ends of the threaded rotating bar 807 and the first limiting slide bars 806 are respectively provided with an anti-off block 809, the bottom end face of the anti-off block 809 is movably abutted against the lifting panel 805, and the bottom end of the threaded rotating bar 807 rotates to extend to the inner wall of the limiting box 7 and fixedly covers the outer wall of the limiting box and is provided with a first conical tooth A wheel 808, a first bevel gear 808 being in movable engagement with the shift drive assembly 804;

when the micro material storage tube group 9 is installed, the extrusion block 904 is embedded into the embedding groove 810 formed in the lifting panel 805 from the side, the forward rotation of the first bevel gear 808 is indirectly controlled to drive the extrusion block 904 in threaded connection with the first bevel gear to slide downwards along the first limiting slide rod 806, the extrusion block 904 slides downwards to extrude the extrusion block 904 embedded with the extrusion block 904 to move downwards, the extrusion block 904 moves downwards to enable the pressure in the micro material storage tube group 9 to be gradually increased until the pressure in the micro material storage tube group 9 is constant to a preset value, meanwhile, the micro-material storage tube group 9 is pressed to be more stably installed, the aim is achieved by indirectly controlling the forward direction of the first bevel gear 808, the lifting panel 805 moves upwards until the lifting panel is abutted to the anti-falling block 809 by indirectly controlling the reverse rotation of the first bevel gear 808, so that the lifting panel 805 cannot be separated, and the micro material storage tube group 9 can be detached and replaced;

the quantitative adding assembly 803 comprises a second bevel gear 811, the second bevel gear 811 is movably engaged with the switching driving assembly 804, the second bevel gear 811 is fixedly connected with a first rotating rod 812, the first rotating rod 812 is rotatably arranged, one end of the first rotating rod 812, which is far away from the second bevel gear 811, sequentially penetrates through the inner wall of the power box 801 and the outer wall of the limiting box 7, extends into the limiting box 7 and is fixedly sleeved with a first connecting sleeve rod 813, one end of the first connecting sleeve rod 813, which is far away from the first rotating rod 812, is provided with a micro-control motor 814, an output shaft of the micro-control motor 814 is fixedly sleeved with a driving gear 815, the driving gear 815 is movably engaged and connected with a quantitative gear 816, a plurality of quantitative gears 816 are arranged, the quantitative gears 816 are distributed according to an annular array by taking the central axis of the hollow rotating rod 821, and the quantitative gears 816 are arranged in one-to-one correspondence with the micro-material storage tube group 9;

the quantitative gear 816 is rotatably sleeved with a hollow rotating rod 821, the hollow rotating rod 821 is fixedly arranged on the bottom wall of the limiting box 7, two ends of the quantitative gear 816 are abutted with limiting sealing wheels 818, the limiting sealing wheels 818 are fixedly sleeved on the outer end of the hollow rotating rod 821, the top end of one of the limiting sealing wheels 818 is provided with a clamping elastic sleeve 819, the clamping elastic sleeve 819 is clamped with the bottom of the liquid storage tube 901, the outer end of the limiting sealing wheel 818 is fixedly connected with a fixed arc sleeve 820, one end, far away from the limiting sealing wheel 818, of the fixed arc sleeve 820 is fixedly connected with the inner wall of the limiting box 7, the quantitative gear 816 is provided with a plurality of L-shaped leakage holes 817, the L-shaped leakage holes 817 are movably communicated with the liquid outlet 905, the L-shaped leakage holes 817 are provided with a side flow port, the side flow ports are movably communicated with the liquid outlet 905 through the L-shaped leakage holes 817, the plurality of L-shaped leakage holes 817 are distributed according to a slip ring shape array by taking the center of the circle of the quantitative gear 816, the aperture of the L-shaped leakage hole 817 is enlarged in equal ratio, the maximum aperture of the L-shaped leakage hole 817 is equal to the aperture of the liquid outlet 905, and the apertures of the liquid outlet 905 and the side flow port are arranged in equal size;

when different trace additive liquid is required to be quantitatively added, the second bevel gear 811 is indirectly controlled to rotate in a single direction to drive the first rotating rod 812 fixed with the first rotating rod 812, the first rotating rod 812 drives the first connecting sleeve rod 813 fixed with the first rotating rod to rotate, the micro-control motor 814 mounted on the first connecting sleeve rod 813 after rotating deflects, the micro-control motor 814 drives the driving gear 815 fixedly sleeved with the output shaft of the micro-control motor to deflect after being deflected, the driving gear 815 is meshed with the quantitative gear 816 and drives the micro-control gear to rotate, the different quantitative gears 816 are micro-material storage tube groups 9 storing different trace additive liquid for target ingredients correspondingly, then the control panel 102 selects a target L-shaped leakage hole 817 with a proper aperture according to the required ingredients, then the micro-control motor 814 is started to work and controls the output shaft of the micro-control motor 814 to rotate to a fixed number of turns, the output shaft of the micro-control motor 814 drives the quantitative gear 816 meshed with the quantitative gear 816 to rotate by a fixed angle after rotating to a fixed number of turns, after the quantitative gear 816 rotates by a quantitative angle, at this time, a target L-shaped leak 817 of the quantitative gear 816 is just aligned with the liquid outlet 905, so that the liquid outlet 905, the L-shaped leak 817 and a side port formed in the hollow rotating rod 821 are sequentially communicated, a target trace additive solution is added into the hollow rotating rod 821 through the liquid outlet 905, the L-shaped leak 817 and the side port, and then the target trace additive solution flows into the diversion hopper 6 through the hollow rotating rod 821, after the target trace additive solution is added, the above steps are repeated, different target trace additive solutions requiring batching are sequentially added into the diversion hopper 6, and then the diversion hopper 6 is gathered into the suspension centrifugal box 2 through the diversion pipe 12, so that the quantitative control adding work of different trace additive solutions is completed, the pore diameter of the L-shaped leak 817 is increased in an equal ratio, and the quantitative adding of the trace additive solution is easy to control;

the working principle is as follows:

step one, inserting a micro-material storage tube group 9 from a top opening formed in a suspension centrifugal box 2 according to a formula of tissue culture requirements, then embedding a pressing block 904 side of the micro-material storage tube group 9 into a clamping groove 810 of a lifting panel 805 of a constant pressure control assembly 802, then sequentially installing the micro-material storage tube group 9 which is required to store target micro additive liquid according to the formula of the requirements, then placing tissue blocks of orchid into a tissue placing opening 501 formed in a tissue culture installation box 5, supporting the tissue blocks of the orchid by a liquid seepage net 502 at the bottom of the tissue placing opening 501, then opening a hinged arc door 104, placing the tissue culture installation box 4 into the top of the tissue culture installation box 5, supporting the tissue culture installation box 4 up the tissue culture net 5, and closing the hinged arc door 104 after the tissue culture installation box 5 is placed;

step two, after the micro-material storage pipe is installed, a liquid inlet throttle valve 14 is opened to enable main liquid of the formula to flow into the suspension centrifugal box 2 from the outside through a liquid inlet pipe 13, and meanwhile, a switching driving assembly 804 and a constant pressure control assembly 802 are controlled to conduct transmission work, so that micro-addition liquid pressure in an extrusion micro-material storage pipe group 9 is constant, the micro-material storage pipe group 9 is further embedded into a quantitative addition assembly 803, and the stability of the micro-material storage pipe group 9 is improved;

when the pressure of the trace additive in the micro-material storage tube group 9 reaches a preset value, the switching drive assembly 804 is controlled to be separated from the constant pressure control assembly 802 and is in transmission connection with the quantitative addition assembly 803, then after the quantitative addition assembly 803 works, the diversion hopper 6 is in through connection with the micro-material storage tube group 9, so that different corresponding trace additive solutions in different micro-material storage tube groups 9 sequentially and quantitatively flow into the diversion hopper 6 according to the formula requirement, and then the trace additive solutions are guided into the suspension centrifugal box 2 through the diversion tube 12 in through connection with the lowest point of the diversion hopper 6;

thirdly, after the mixed liquid in the suspension centrifugal box 2 reaches a preset height, starting the centrifugal pushing assembly 3 to work, further mixing and scattering the mixed liquid, fully contacting the mixed liquid and generating suspension, wherein the suspension is suspended on the top surface of the mixed liquid and then gradually pushed to the periphery from the middle part of the mixed liquid, and at the moment, starting the distribution liquid pump 10 to continuously suck the suspension through a conduit and equally distribute the suspension to the tissue culture installation boxes 4 which are equidistantly arranged through the distribution pipe 11, and continuously pushing the suspension to the connection part of the distribution liquid pump 10 under the action of the centrifugal pushing assembly 3, so that the suspension is more conveniently sucked; wherein organize all to install the check valve that prevents the suspension backward flow on the install bin 4 tissue culture, prevent liquid intercommunication effect, the check valve is installed in the tip of the distributing pipe 11 far away from distributing liquid pump 10, the quantity of check valve and the quantity adaptation of group tissue culture install bin 4, the check valve is not drawn.

Step four, after the suspension flows into the tissue culture installation box 4 and is gradually increased, the suspension gradually rises and flows into the tissue placement opening 501 through the liquid seepage net 502, when the suspension covers the local part of the tissue block of the orchid, the distribution liquid pump 10 is closed, the suspension in the tissue block of the orchid gradually becomes less in the process of rooting, sprouting and growing gradually, so that the distribution liquid pump 10 needs to be opened in real time to convey the suspension, the suspension is quantified, the suspension consumed in the process of rooting, sprouting and growing of the tissue block of the orchid is different, the suspension needs to be dynamically sucked according to the growth period of the tissue culture of the orchid seedling, the liquid in the tissue culture installation box 4 is kept in a quantified state, and the full and rapid growth of the orchid seedling in the tissue culture process is ensured;

step five, after the tissue culture of the orchid seedlings is finished, opening the hinged arc door 104, then taking the tissue culture screen plate 5 and replacing the tissue culture screen plate 5 with a new tissue culture screen plate 5 in which orchid tissue blocks are placed, and thus continuously collecting the orchid seedlings which are subjected to tissue culture growth;

by integrating the technical scheme, the tissue culture device realizes the efficient generation of the tissue culture of orchid seedlings on the basis of the continuous tissue culture of the orchid seedlings, and realizes the micro-pressure control of the micro-addition liquid, the quantitative control of the addition of the micro-addition liquid and the selective control of the micro-addition liquid with different requirements and formulas by the cooperation of the constant-pressure control component 802, the quantitative addition component 803 and the switching drive component 804, so that the tissue culture device can be widely adapted to different nutritional requirements of various tissue tissues of orchid, and the tissue culture device has a wider tissue culture application range.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.

Claims

1. An orchid seedling tissue culture device based on intelligent regulation comprises a tissue culture incubator (1), a suspension centrifugal box (2) and a tissue culture installation box (4) are fixedly arranged in the tissue culture incubator (1), it is characterized in that the suspension centrifugal box (2) is arranged on the bottom wall of the tissue culture constant temperature box (1), and the suspension centrifugal box (2) is provided with a centrifugal pushing component (3), the centrifugal pushing component (3) is arranged on the tissue culture incubator (1), a plurality of tissue culture installation boxes (4) are arranged at equal intervals, the tissue culture screen (5) is movably clamped on the top surface of the tissue culture installation box (4), the tissue culture net plate (5) is provided with a plurality of tissue placing openings (501) for placing orchid tissues, a liquid seepage net (502) for supporting the orchid tissue is arranged at the bottom end of the tissue placing port (501), and the liquid seepage net (502) is fixedly arranged at the bottom end of the tissue culture net plate (5);

the top of the suspension centrifugal box (2) is connected with a distribution liquid pump (10) through a pipeline in a through manner, a liquid inlet of the distribution liquid pump (10) is connected with the top of the suspension centrifugal box (2) in a through manner, a liquid outlet (905) of the distribution liquid pump (10) is connected with a distribution pipe (11) through manner, one end, far away from the distribution liquid pump (10), of the distribution pipe (11) is connected with the center of the bottom end of the tissue culture installation box (4) in a through manner, two sides of the bottom of the suspension centrifugal box (2) are connected with a guide pipe (12) and a liquid inlet pipe (13) through manner, a liquid inlet throttle valve (14) is installed on the liquid inlet pipe (13), one end of the guide pipe (12) is connected with a guide hopper (6) through manner, the top end of the guide hopper (6) is fixedly connected with a limiting box (7), the limiting box (7) is connected with the guide hopper (6) in a through manner, and the limiting box (7) is provided with a micro-adding control structure (8) and a micro-material storage pipe group (9), add control structure (8) a little and add subassembly (803) and switch drive assembly (804) including headstock (801), constant voltage control subassembly (802), ration, headstock (801) is fixed to be located the top of spacing case (7), the fine material is stored nest of tubes (9) and is inlayed in spacing case (7) and rather than swing joint, fine material is stored nest of tubes (9) and is equipped with a plurality ofly, and fine material is stored nest of tubes (9) and is used the centre of a circle of spacing case (7) as the center and distribute according to annular array, fine material is stored nest of tubes (9) and is added subassembly (803) adaptation setting with the ration, constant voltage control subassembly (802) and fine material are stored nest of tubes (9) adaptation butt, constant voltage control subassembly (802) and ration are added subassembly (803) and are all connected with switch drive assembly (804) active drive.

2. The orchid seedling tissue culture device based on intelligent regulation and control as claimed in claim 1, wherein the centrifugal pushing assembly (3) is composed of a centrifugal motor (201), a centrifugal rotating rod (202), a centrifugal sleeve (203), centrifugal fan blades (204), a pushing sleeve (205) and a pushing rod (206), the centrifugal motor (201) is fixedly arranged at the bottom end of the tissue culture incubator (1), the centrifugal rotating rod (202) is rotatably arranged in the suspension centrifugal box (2), one end of the centrifugal rotating rod (202) sequentially penetrates through the inner wall of the suspension centrifugal box (2) and the inner wall of the tissue culture incubator (1) to extend to the outside of the tissue culture incubator and is fixedly connected with the output shaft of the centrifugal motor (201), the outer end of the centrifugal rotating rod (202) is provided with the centrifugal sleeve (203) and the pushing sleeve (205), the outer end of the centrifugal sleeve (203) is fixedly provided with the centrifugal fan blades (204), and the centrifugal fan blades (204) are arranged at the bottom of the centrifugal fan blades (204), the outer end of the pushing sleeve (205) is fixedly sleeved with a pushing rod (206), and the pushing rod (206) is arranged at the top of the centrifugal fan blade (204).

3. The orchid seedling tissue culture device based on intelligent regulation and control of claim 1, wherein the micro-material storage tube group (9) is composed of a liquid storage tube (901), a sliding block (902), a telescopic rod (903) and an extrusion block (904), the sliding block (902) is arranged in the liquid storage tube (901) in a sliding mode, the top end of the sliding block (902) is fixedly connected with the telescopic rod (903), the top end, far away from the sliding block (902), of the telescopic rod (903) slides to penetrate through the top wall of the liquid storage tube (901) to extend to the outside of the liquid storage tube and is fixedly connected with the extrusion block (904), and a liquid outlet (905) is formed in the position, far away from the center, of the bottom end face of the liquid storage tube (901).

4. The orchid seedling tissue culture device based on intelligent regulation and control as claimed in claim 3, wherein the constant pressure control assembly (802) comprises a lifting panel (805), the lifting panel (805) is provided with a plurality of embedding grooves (810), the embedding grooves (810) are arranged on the outer ring side of the bottom of the lifting panel (805), the embedding grooves (810) are distributed according to a circular array by taking the circle center of the lifting panel (805) as the center, the pressing blocks (904) are movably embedded in the embedding grooves (810), the lifting panel (805) is sleeved with a first limiting slide bar (806) and a threaded rotating bar (807), the first limiting slide bar (806) is connected with the lifting panel (805) in a sliding manner, the first limiting slide bar (806) is provided with a plurality of limiting slide bars, the first limiting slide bar (806) is distributed according to a circular array by taking the circle center of the lifting panel (805) as the center, the bottom end of the first limiting slide bar (806) is fixedly connected with the top end face of the limiting box (7), the thread rotating rod (807) is in threaded connection with the lifting embedded plate (805), anti-falling blocks (809) are arranged at the top ends of the thread rotating rod (807) and the first limiting sliding rod (806), the bottom end face of each anti-falling block (809) is movably abutted to the lifting embedded plate (805), the bottom end of the thread rotating rod (807) rotates to penetrate through the outer wall of the limiting box (7) to extend into the outer wall of the limiting box and is fixedly sleeved with a first bevel gear (808), and the first bevel gear (808) is movably meshed with the switching driving component (804).

5. The orchid seedling tissue culture device based on intelligent regulation and control as claimed in claim 4, wherein the quantitative adding component (803) comprises a second bevel gear (811), the second bevel gear (811) is movably meshed with the switching driving component (804), a first rotating rod (812) is fixedly connected with the second bevel gear (811), the first rotating rod (812) is rotatably arranged, one end, far away from the second bevel gear (811), of the first rotating rod (812) sequentially penetrates through the inner wall of the power box (801) and the outer wall of the limiting box (7) to extend into the limiting box (7) and is fixedly sleeved with a first connecting sleeve rod (813), one end, far away from the first rotating rod (812), of the first connecting sleeve rod (813) is provided with a micro-control motor (814), an output shaft of the micro-control motor (814) is fixedly sleeved with a driving gear (815), and the driving gear (815) is movably meshed with a quantitative gear (816), the quantitative gear (816) is provided with a plurality of quantitative gears (816), the quantitative gears (816) are distributed in an annular array by taking the central axis of the hollow rotating rod (821) as the center of a circle, the quantitative gears (816) and the micro-material storage tube groups (9) are arranged in a one-to-one correspondence manner, the hollow rotating rod (821) is sleeved on the quantitative gears (816) in a rotating manner, the hollow rotating rod (821) is fixedly arranged on the bottom wall of the limiting box (7), two ends of the quantitative gears (816) are abutted with limiting sealing wheels (818), the limiting sealing wheels (818) are fixedly sleeved on the outer ends of the hollow rotating rod (821), the top end of one of the limiting sealing wheels (818) is provided with a clamping elastic sleeve (819), the clamping elastic sleeve (819) is clamped with the bottom of the liquid storage tube (901), the outer end of each limiting sealing wheel (818) is fixedly connected with a fixed arc sleeve (820), one end, far away from the limiting sealing wheel (818), of each fixed arc sleeve (820) is fixedly connected with the inner wall of the limiting box (7), l shape small opening (817) have been seted up in ration gear (816), L shape small opening (817) and liquid outlet (905) activity through connection, L shape small opening (817) and hollow bull stick (821) have seted up the side stream mouth, the side stream mouth passes through L shape small opening (817) and liquid outlet (905) activity through connection.

6. The orchid seedling tissue culture device based on intelligent regulation and control as claimed in claim 5, wherein a plurality of L-shaped leakage holes (817) are arranged, the L-shaped leakage holes (817) are distributed in a slip ring array by taking the circle center of the quantitative gear (816) as the center, the aperture of the L-shaped leakage holes (817) is enlarged in an equal ratio, the maximum aperture of the L-shaped leakage holes (817) is equal to the aperture of the liquid outlet (905), and the aperture of the liquid outlet (905) and the aperture of the side flow port are arranged in an equal size.

7. The orchid seedling tissue culture device based on intelligent regulation and control of claim 5, wherein the switching drive assembly (804) comprises a lifting box (823), the lifting box (823) is slidably arranged in the power box (801), the lifting box (823) is provided with a lifting air rod (822), a second connecting loop rod (827) and a return spring (829), the lifting air rod (822) is installed on the bottom wall of the power box (801), one end of the second connecting loop rod (827) is fixedly connected with the power box (801), the other end of the second connecting loop rod (827) is sleeved with a second limiting slide rod (828), the second limiting slide rod (828) is fixedly arranged in the power box (801), the return spring (829) is fixedly arranged at the bottom end of the power box (801), and the other end of the return spring (829) is fixedly arranged at the bottom wall of the power box (801), the power box (801) is internally and fixedly provided with a servo motor (824), an output shaft of the servo motor (824) is fixedly connected with a second rotating rod (825), the second rotating rod (825) is rotatably arranged in the lifting box (823), one end of the second rotating rod (825) penetrates through the inner wall of the lifting box (823) to extend to the outside of the lifting box and fixedly sleeve a third bevel gear (826), the first bevel gear (808) and the second bevel gear (811) are arranged oppositely, and the first bevel gear (808), the second bevel gear (811) and the third bevel gear (826) are respectively and movably engaged.

8. The orchid seedling tissue culture device based on intelligent regulation and control as claimed in claim 1, wherein a base (101) is installed at the bottom of the tissue culture incubator (1), a plurality of hinged arc doors (104) are installed on the outer wall of the tissue culture incubator (1), a hinged arc door (104) is correspondingly arranged at each tissue culture installation box (4), a door handle (105) is installed on each hinged arc door (104), a control panel (102) is installed on the tissue culture incubator (1), and a starting button (103) and a display screen are installed on each control panel (102).