CN114631534A - Exogenous mixture for improving yield of wheat infected by flowering period, application method and application thereof - Google Patents
Exogenous mixture for improving yield of wheat infected by flowering period, application method and application thereof Download PDFInfo
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Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/26—Phosphorus; Compounds thereof
Abstract
The invention belongs to the technical field of wheat cultivation, and particularly relates to an exogenous mixture for improving yield of wheat infected by a seed-soaking disease in a flowering period, an application method and application thereof. The exogenous mixture is a mixed aqueous solution containing 6-BA and monopotassium phosphate, wherein the concentration of the 6-BA is 0.01-0.02mmol/L, and the concentration of the monopotassium phosphate is 0.01-0.05 mol/L. The exogenous compound agent has obvious effect on spring wheat, effectively relieves the influence of waterlogging on the grain number per ear and the thousand grain weight of the wheat, improves the phenomena of difficult selection of a single repairing agent and flaky use effect for a long time, is sprayed when being stressed by the waterlogging in spring, can obviously improve the waterlogging tolerance of the wheat, and repairs the result influence of reduced yield of the wheat caused by the waterlogging.
Description
Technical Field
The invention belongs to the technical field of wheat cultivation, and particularly relates to an exogenous mixture for improving yield of wheat infected by a seed-soaking disease in a flowering period, an application method and application thereof.
Background
Wheat is one of three major food crops in the world, and about 40 percent of people in China use wheat as a main food, and the wheat plays an important role in national economy. In recent years, the incidence of wheat stains in this region tends to be markedly increased in the growing season. It is pointed out that the waterlogging is the main meteorological disaster factor in the middle and later growth period of the wheat in the region. Excess precipitation and the imbalance in the spatial and temporal distribution of precipitation lead to reduced yield in the wheat in this region. Statistical data indicate that Anhui wheat has a 10% reduction in waterlogging from 1981 to 2005 annually, and about every 2.5 years, and that Anhui wheat lost more than 20% in 1985, 1991, 1998, and 2003. The waterlogging becomes a main limiting factor for improving the wheat yield in the region.
When the wheat is infected with the water stain in spring, the plant growth regulating compound substance is sprayed on the wheat, which is beneficial to inducing the wheat to generate a series of physiological and biochemical reactions, can effectively improve the self-resistance of the wheat and has very important effect on stress resistance and stable yield of the wheat. At present, most of wheat is improved in stain resistance by applying a traditional fertilizer and a single repairing agent (such as urea and ABA), and the repairing effect is different for wheat of different varieties and different regions.
Researches on the reduction of stain damage by exogenous plant growth regulators are frequently repeated, for example, patent CN111955471B discloses a method for improving the stain resistance and negative resistance of wheat and an application thereof, progesterone is added with water to prepare a progesterone solution with the concentration of 0.05-1 mu mol/L; and spraying the prepared progesterone solution to wheat. Patent CN111820087A discloses a method for synergistically improving the stain resistance and negative resistance of wheat and application thereof, which comprises the steps of adding water into progesterone and chitosan oligosaccharide to prepare a mixed solution of 0.05-1 mu mol/L progesterone and 150-1000mg/L chitosan oligosaccharide; and spraying the prepared mixed solution of the progesterone and the oligochitosan on the wheat. When the wheat is infected with waterlogging, the method of the patent helps the wheat to respond to a waterlogging-resistant mechanism better, can effectively reduce the waterlogging-resistant degree of the wheat and enhance the waterlogging resistance or the waterlogging resistance and the negative resistance of the wheat. But the stain repairing effect on wheat needs to be improved.
In addition, research shows that phosphorus (P) and potassium (K) are important nutrient elements which obviously influence the grain weight and the yield of wheat, 6-BA spraying can well promote cell division, and the substances are mostly used for regulating the growth of crops in the prior art. For example, patent CN105767001B discloses a conditioner for improving low temperature resistance of wheat, which comprises, by mass, 98% of 6-BA 5-10 parts, 70% of naphthylacetate 10-15 parts, 96% of mepiquat chloride 20-30 parts, 40% of ethephon 10-20 parts and 98% of monopotassium phosphate 20-30 parts; is used for improving the low temperature resistance of wheat and reducing the damage of low temperature cold damage to wheat in the later growth stage. Patent CN102701867A discloses a preparation for improving the fruit setting rate of tomatoes, which comprises 0.02-0.03% of alpha-naphthylacetic acid, 0.02-0.04% of anti-drop agent, 0.08-0.1% of compound sodium nitrophenolate, 0.03-0.06% of 6-BA, 4-8% of potassium dihydrogen phosphate, 3-5% of calcium chloride, 0.02-0.05% of zinc sulfate and the balance of water; is used for improving the fruit setting rate and fruit expansion speed of tomatoes. Patent CN106220369A discloses a conditioning agent for controlling excessive growth, promoting expansion and increasing yield of potatoes, which comprises 4-8 parts of mepiquat chloride with the mass concentration of 96%, 30-50 parts of choline chloride with the mass concentration of 50%, 3-5 parts of 6-BA with the mass concentration of 98%, 5-15 parts of urea and 5-15 parts of monopotassium phosphate; is used for promoting the expansion of potato tubers and the accumulation of starch and increasing the yield of the potato tubers.
The research reports are mostly carried out on specific crops or under specific environmental conditions, and the raw material components and the dosage of the conditioner are different, so that the displayed effects are completely different.
Disclosure of Invention
The invention aims to solve the problems that the existing exogenous plant growth regulator improves the stain resistance of wheat by using a traditional fertilizer and a single repairing agent (such as urea and ABA), and has uneven effects for different wheat varieties and wheat in different regions, and provides an exogenous compound agent for improving the yield of the wheat suffering from the stains in the flowering period, an application method and application thereof.
In order to achieve the above object, the present invention discloses an exogenous mixture for increasing the yield of the wheat which is infected by the seed-bearing water during the flowering period, wherein the exogenous mixture is a mixed aqueous solution containing 6-BA and monopotassium phosphate, the concentration of the 6-BA is 0.01-0.02mmol/L, and the concentration of the monopotassium phosphate is 0.01-0.05 mol/L.
As a preferable scheme, the concentration of 6-BA in the exogenous mixture agent is 0.01mmol/L, and the concentration of potassium dihydrogen phosphate is 0.02 mol/L.
Preferably, the exogenous mixture further comprises a surfactant, and the concentration of the surfactant is 0.1-0.5% (V/V).
Preferably, the concentration of the surfactant is 0.3% (V/V).
Preferably, the surfactant is Tween-20.
The invention also discloses an application method of the exogenous mixture for improving the yield of the wheat suffered from the waterlogging in the flowering period, and when the wheat suffered from the waterlogging, the exogenous mixture is used for spraying the leaves of the wheat until the two sides of the leaves fall down.
Preferably, the spraying time of the exogenous mixture agent is afternoon on a sunny day.
Preferably, the waterlogging refers to an agricultural meteorological disaster in which soil is supersaturated in water content.
The invention also discloses application of the exogenous mixture for improving the yield of the wheat suffering from the waterlogging during the flowering period to improve the waterlogging resistance of Yangmai 18.
The spraying method comprises the following steps: spraying 3 times before and 5 days after the waterlogging treatment on the same day after the waterlogging treatment.
Through a large amount of researches and years of experiments, the invention discovers that the stain resistance of plants can be obviously improved by spraying plant growth regulating substances to the plants. Collecting wheat in flowering period after waterloggingThe 6-BA and potassium dihydrogen phosphate compound agent is used, so that the malondialdehyde content of flag leaves can be remarkably reduced, the cell division and substance accumulation in organisms can be promoted, the senescence can be prevented, the chlorophyll content can be improved, the stain resistance of wheat can be enhanced, the self-protection capability and photosynthesis of the flag leaf cells of the wheat in the later growth period can be improved, and the grain weight and the yield can be improved. Wherein, the spraying of monopotassium phosphate on the leaf surface obviously improves the activities of SOD, POD and CAT of flag leaves, reduces the MDA content, relieves the premature senility of plants, increases the active filling period and the grain weight, and applies KH on the leaf surface2PO4Is an effective measure for improving the grain weight of wheat; the spraying of 6-BA obviously improves the decline of the photosynthetic function after the flowers caused by the waterlogging after the flowers, and improves the quantity of assimilated dry matter input sub-grains and the contribution rate of the assimilated dry matter input sub-grain yield.
Compared with the prior art, the invention has the following advantages:
(1) the exogenous compound agent has obvious effect on spring wheat, effectively relieves the influence of waterlogging on the grain number per ear and the thousand grain weight of the wheat, improves the phenomena of difficult selection of a single repairing agent and flaky use effect for a long time, is sprayed when being stressed by the waterlogging in spring, can obviously improve the waterlogging tolerance of the wheat, and repairs the effect influence of the result of wheat yield reduction caused by the waterlogging;
(2) according to the invention, 6-BA and monopotassium phosphate are selected for compounding in a specific proportion, the two substances can respectively induce wheat to generate a series of physiological and biochemical reactions capable of effectively improving the wheat stain resistance, the 6-BA can improve the assimilation of the quantity of dry substance input kernels and the contribution rate of the assimilation dry substance input kernels to the yield of the kernels by relieving the decline of the photosynthetic function of leaves caused by stain damage, the stain resistance of the wheat is enhanced, the senescence caused by the stain water is reduced, and the damage degree of the stain damage to wheat plants is relieved; the monopotassium phosphate obviously improves the activities of SOD, POD and CAT of flag leaves, reduces the MDA content, delays the premature senility of plants, and increases the active filling period and the grain weight, thereby achieving the effect of repairing the damaged wheat; the two substances jointly increase the stain resistance of wheat by regulating multiple action mechanisms such as an antioxidant enzyme system, a penetration regulating substance and the like, and the two repairing agents mutually promote to have the effect that the overall effect is larger than the single effect;
(3) the surfactant Tween-20 is added, and the macromolecules have hydrophilic parts and lipophilic parts on the molecules, so that the macromolecules can promote plants to absorb the macromolecules which cannot be dissolved in water, can help water to permeate a certain high-fat biological membrane, and effectively improves the effect of the compound agent.
Drawings
FIG. 1 shows the precipitation and the average temperature in 5 months in 2020 to 2021 of test base in the example;
FIG. 2 is a graph of wheat growth in the examples;
fig. 3 is a spray application used in the examples.
Detailed Description
The invention is further illustrated with reference to the following figures and examples.
Example 1
Preparing an exogenous mixture: mixing 6-BA, monopotassium phosphate and water, adding Tween-20, and uniformly mixing to obtain an exogenous mixture; wherein the concentration of 6-BA is 0.01mmol/L, the concentration of potassium dihydrogen phosphate is 0.02mol/L, and the concentration of Tween-20 is 0.3% (V/V).
Example 2
Preparing an exogenous mixture: mixing 6-BA, potassium dihydrogen phosphate and water, adding Tween-20, and mixing to obtain exogenous mixture; wherein the concentration of 6-BA is 0.02mmol/L, the concentration of potassium dihydrogen phosphate is 0.05mol/L, and the concentration of Tween-20 is 0.1% (V/V).
Example 3
Preparing an exogenous mixture: mixing 6-BA, monopotassium phosphate and water, adding Tween-20, and uniformly mixing to obtain an exogenous mixture; wherein the concentration of 6-BA is 0.015mmol/L, the concentration of potassium dihydrogen phosphate is 0.01mol/L, and the concentration of Tween-20 is 0.5% (V/V).
Using the exogenous combination of example 1 as an example, experimental descriptions were made:
the test is carried out at a comprehensive test station (Dongding 117 degrees, 01 degrees and north latitude 30 degrees, 57') in Anhui province, Guo river, Zhen Anhui agriculture university, Lujiang province, Hefei city, Anhui province, 5 months from 2020 to 2021, and the variety to be tested is Yangmai 18. The pH value of the soil is 6.30, wherein the organic matter content is 23.41g/kg, the total nitrogen content is 0.99g/kg, the alkaline hydrolysis nitrogen content is 121.00mg/kg, the effective phosphorus content is 33.60g/kg, and the quick-acting potassium content is 356.00 mg/kg. PVC pipes with the diameter of 11cm and the length of 100cm are selected for the test, field surface soil is taken, screened and filled into the pipes, and the pipes are settled with water and placed on a customized planting pipe frame. The sowing time is 11 months and 1 day in 2020. Before sowing, 80g of organic fertilizer, 0.7g of compound fertilizer and 0.2g of urea are applied to each tube. 3 seeds are sowed in each tube, wheat is fixed after three leaves, one seed is left in each tube, and 0.19g of pure nitrogen is applied in the jointing stage of the wheat. Other management measures are carried out according to the high-yield requirement of the field. The precipitation and the average temperature in the month from 11 months in 2020 to 5 months in 2021 in the test base are shown in FIG. 1.
The method is characterized in that a waterless treatment (W0) and a waterlogging treatment (W9) are arranged at the flowering period of the wheat, an exogenous mixture (T1) is sprayed for three times respectively at the flowering period (before the waterlogging treatment), the 5 th day after the flower (in the waterlogging treatment) and the 9 th day after the flower (on the day after the waterlogging treatment), and plants sprayed with equal amount of water are used as a control (T0) and are sprayed until the two sides of the leaves fall. The SPAD value, net photosynthetic rate and chlorophyll fluorescence parameter of flag leaf were measured at 9 days after the flower (day of waterlogging treatment), 16 days after the flower (7 days after waterlogging treatment) and 23 days after the flower (14 days after waterlogging treatment). Investigation sampling of root system samples is carried out on the 9 th day (the day after waterlogging treatment) and the 16 th day (the 7 th day after waterlogging treatment) after flowers, the PVC pipe is taken out during sampling, soil in the PVC pipe is washed by a high-pressure water gun, the root system is washed clean, then the root system and the overground part are separated rapidly, and all indexes are stored and measured in a layered mode after cleaning. Taking 6 tubes of samples in each treatment, wherein 3 tubes are used for measuring the root form index, and scanning and measuring the root form index by using a root form scanner after the samples are taken out; and 3 tubes for measuring physiological indexes of the root system, taking out the sample, quickly placing the sample into liquid nitrogen for treatment, and then placing the sample into a freezer at the temperature of-80 ℃ for storage, wherein the freezer is used for measuring the activity of related enzymes. After the wheat is ripe, the number of wheat ears, the number of grains per ear and the weight of thousand grains are measured, and the wheat is weighed and the yield is calculated.
1.1 root form
The influence of spraying exogenous mixture on the average diameter of the wheat root system during the waterlogging stress is shown in table 1.
TABLE 1 influence of spraying of the built-up agent on the mean diameter of the root system of wheat
As can be seen from the test data in Table 1, when the exogenous mixture is sprayed during the period of flowering, the average root diameter is significantly higher than that of the control treatment (P <0.05) at 9 and 16 days after the flowers are bloomed. At 9 and 16 days after the flowers are treated with waterlogging for 9 days and treated without waterlogging, the average diameter of the root system treated by T1 is obviously larger than that treated by T0 in each soil layer sample. Therefore, when the exogenous compound agent is sprayed when the wheat in the flowering period is stressed by waterlogging, the average diameter of the root system of the wheat can be obviously improved, and the growth and development of the root system of the wheat can be enhanced.
The influence of spraying exogenous compound agent on the total root length of wheat root system during waterlogging stress is shown in table 2.
TABLE 2 influence of exogenous mixture spraying on total root length of wheat root system in flowering period
As can be seen from Table 2, after 9 days and 16 days of florescence, the waterlogging treatment and the waterlogging-free treatment are carried out, and the total root length of the root system treated by T1 in each soil layer sample is obviously higher than that treated by T0. Therefore, when the exogenous compound agent is sprayed when the wheat in the flowering period is stressed by waterlogging, the total root length of the wheat root system can be obviously improved, and the growth and development of the wheat root system are enhanced.
The influence of spraying exogenous compound agent on the total volume of wheat root system during the waterlogging stress is shown in table 3.
TABLE 3 influence of spraying exogenous built-up agent on the total volume of wheat root system in flowering period
As can be seen from Table 3, the treatment of the waterlogging water for 9 days and the treatment of the waterlogging water for 16 days after the florescence, and the total volume of the root system treated by T1 in each soil layer sample is obviously higher than that treated by T0. Therefore, when the compound agent is sprayed when the wheat in the flowering period is stressed by waterlogging, the total volume of the root system of the wheat can be obviously improved, and the growth and development of the root system of the wheat can be enhanced.
1.2 root antioxidases
The influence of spraying exogenous compound agent on wheat root system antioxidant enzyme during waterlogging stress is shown in tables 4 and 5.
TABLE 4 influence of spraying exogenous mixture on superoxide dismutase of wheat root system
As can be seen from Table 4, the SOD of the root system treated by T1 in each soil layer is obviously higher than that treated by T0 in 9 days of waterlogging treatment and no waterlogging treatment at 9 days and 16 days after the flowers are bloomed. Therefore, when the wheat in the flowering period is stressed by waterlogging, the exogenous compound agent is sprayed, the SOD activity of the wheat root system can be obviously improved, and the waterlogging tolerance of the wheat can be enhanced.
TABLE 5 influence of spraying exogenous built-up agent on peroxidase of wheat root system
As can be seen from Table 5, the treatment of the waterlogging water for 9 days and the treatment of the waterlogging water for 16 days after the florescence, the root POD of the T1 treatment in each soil layer is significantly lower than that of the T0 treatment. Therefore, when the exogenous compound agent is sprayed when the wheat in the flowering period is stressed by the waterlogging damage, the POD activity of the root system of the wheat can be obviously reduced, and the waterlogging tolerance of the wheat is enhanced.
1.3 flag leaf SPAD value
The effect of spraying exogenous combination on SPAD value of wheat flag leaf is shown in table 6.
TABLE 6 influence of spraying exogenous built agent on SPAD value of wheat flag leaf
As can be seen from Table 6, the SPAD values of the flag leaves treated by T1 were significantly higher than those of the T0 at 9, 16 and 23d after the flower for the treatment of waterlogging for 9 days and the treatment of no waterlogging. The spraying of the exogenous mixture obviously improves the photosynthetic capacity of the flag leaves of the wheat, so that the wheat can keep higher photosynthetic capacity when being soaked, and the stain resistance of the wheat is improved.
1.4 Tansliaea net photosynthetic Rate
The effect of spraying exogenous compound on the net photosynthetic rate of wheat flag leaves is shown in table 7.
TABLE 7 influence of spraying exogenous built-up agent on net photosynthetic rate of wheat flag leaf
As can be seen from Table 7, the T1-treated flag leaf net photosynthetic rate was significantly higher than that of the T0-treated flag leaf net photosynthetic rate in the treatment periods of 9 days for the waterlogged water and 9, 16 and 23 days after the non-waterlogged water treatment. The spraying of the exogenous mixture obviously improves the photosynthetic property of the flag leaves of the wheat, so that the wheat can keep higher photosynthetic capacity when being soaked, and the stain resistance of the wheat is improved.
1.5 chlorophyll fluorescence Properties of flag leaf
The effect of spraying exogenous built agents on the actual photochemical efficiency of wheat flag leaves is shown in table 8.
TABLE 8 influence of spraying exogenous built-up agent on actual photochemical efficiency of wheat flag leaves
As can be seen from Table 8, the actual photochemical efficiency of flag leaves treated by T1 was significantly higher than that of flag leaves treated by T0 in 9 days of waterlogging treatment and in no-waterlogging treatment at 9, 16 and 23d after flowering. The spraying of the exogenous mixture obviously improves the photosynthetic capacity of the flag leaves of the wheat, so that the wheat can keep higher photosynthetic property when being soaked, and the stain resistance of the wheat is improved.
1.7 yield
The effect of spraying exogenous formulation on yield is shown in table 9.
TABLE 9 influence of exogenous compound agent spraying on wheat yield and its constituent factors
| Treatment of | Spike number (Spike/pipe) | Grain number of Spike (Spike/pipe) | Thousand Kernel weight (g) | Yield (g/pipe) |
| T0(W0) | 6.50a | 49.54a | 42.61b | 13.51b |
| T1(W0) | 7.25a | 52.19a | 48.82a | 17.74a |
| T0(W9) | 8.75a | 46.72a | 41.93b | 12.27b |
| T1(W9) | 9.25a | 47.30a | 48.56a | 16.44a |
As can be seen from Table 9, for the treatment of the waterlogging water for 9 days and the treatment of the waterlogging water for no waterlogging water, the number of the ears and the number of the grains of the ears treated by T0 and T1 have no obvious difference, the weight of the thousand grains treated by the waterlogging water for 0 days and 9 days and T1 is improved by 14.57-15.81 percent compared with that treated by T0, and the yield is improved by 31.31-33.99 percent. The exogenous compound agent is sprayed, so that the thousand-grain weight and the yield of the wheat in the mature period are obviously higher than those of the wheat treated by spraying clear water, and the damage of the wheat caused by the waterlogging is effectively repaired to a certain extent.
According to test results, the compound agent is sprayed by an external source in the waterlogging stress period, the photosynthetic property of flag leaves can be obviously improved, the normal physiological action of wheat is maintained, the influence of waterlogging on the growth and development of the wheat is relieved, the grain maintains higher grouting speed, the yield loss caused by the waterlogging is reduced, and the damage effect on the wheat caused by the waterlogging in the restoration flowering period is obvious.
The foregoing is merely a preferred embodiment of the invention, which is intended to be illustrative and not limiting. It will be understood by those skilled in the art that various changes, modifications and equivalents may be made therein without departing from the spirit and scope of the invention as defined in the appended claims.
Claims (10)
1. An exogenous mixture for improving the yield of wheat infected by the waterlogged wheat in the flowering period, which is characterized in that: the exogenous mixture is a mixed aqueous solution containing 6-BA and monopotassium phosphate, wherein the concentration of the 6-BA is 0.01-0.02mmol/L, and the concentration of the monopotassium phosphate is 0.01-0.05 mol/L.
2. The exogenous combination for increasing yield of a wheat susceptible to infestation at anthesis of claim 1, wherein: the concentration of 6-BA in the exogenous mixture agent is 0.01mmol/L, and the concentration of potassium dihydrogen phosphate is 0.02 mol/L.
3. The exogenous combination for increasing yield of a wheat susceptible to infestation at anthesis of claim 1, wherein: the exogenous mixture also contains a surfactant.
4. An exogenous combination for increasing the yield of a wheat susceptible to spoilage by flowering according to claim 3, wherein: the surfactant is Tween-20.
5. The exogenous combination for increasing yield of a wheat susceptible to infestation at anthesis according to claim 4, wherein: the concentration of the surfactant is 0.1-0.5% (V/V).
6. The exogenous combination for increasing yield of a wheat susceptible to infestation at anthesis according to claim 4, wherein: the concentration of the surfactant was 0.3% (V/V).
7. A method of applying an exogenous combination for increasing the yield of a wheat susceptible to a brood at anthesis as claimed in any one of claims 1 to 6, characterized in that: when the wheat leaves are infected with stain, the exogenous mixture is sprayed on the wheat leaves until the two sides of the wheat leaves fall down.
8. The method of applying an exogenous combination for increasing the yield of wheat susceptible to flowering stage as claimed in claim 7, wherein: the spraying time of the exogenous mixture is afternoon in sunny days.
9. The method of applying an exogenous combination for increasing yield of a wheat susceptible to breeding during anthesis according to claim 7, wherein: the waterlogging refers to an agricultural meteorological disaster with supersaturated soil water content.
10. Use of an exogenous combination according to any one of claims 1 to 6 for increasing the yield of a wheat susceptible to spoilage by flowering, wherein: for improving the stain resistance of yangma 18.
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| CN103314846B (en) * | 2013-03-22 | 2015-05-13 | 西北大学 | Adventitious bud induction method for amygdalus pedunculata pall explants |
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Application publication date: 20220617 |