CN114618420A

CN114618420A - Reaction device capable of automatically controlling liquid

Info

Publication number: CN114618420A
Application number: CN202210464696.8A
Authority: CN
Inventors: 吴礼杰; 吴旭东
Original assignee: Jiangsu Jimbio Technology Co ltd
Current assignee: Jiangsu Jimbio Technology Co ltd
Priority date: 2022-04-29
Filing date: 2022-04-29
Publication date: 2022-06-14

Abstract

The invention discloses a reaction device capable of automatically controlling liquid, and belongs to the technical field of liquid reaction devices. The reaction tube is communicated with each liquid storage tube through an elastic hose; the columnar valve sleeve is arranged in the guide sleeve and is in sliding connection with the guide sleeve, and an elastic part is arranged between the columnar valve and the guide sleeve; the columnar valve is provided with a through hole matched with the elastic hose, and the elastic hose penetrates through the through hole; one end of the guide sleeve, which is close to the elastic hose, is provided with an extrusion end face; the elastic hose can be extruded on the extrusion end surface through the telescopic columnar valve, and a liquid channel in the elastic hose is closed or is communicated. The invention can realize effective opening or closing of the elastic hose, further realize communication or disconnection between the liquid storage pipe and the reaction pipe, effectively control the flow of the reaction liquid, and effectively improve the accuracy of the consumption of the reaction liquid and the experimental efficiency.

Description

Reaction device capable of automatically controlling liquid

Technical Field

The invention belongs to the technical field of liquid reaction devices, and particularly relates to a reaction device capable of automatically controlling liquid.

Background

Reaction reagents are arranged on the micro-fluidic chip, and on-off control is carried out through the valve to realize automatic reaction reagent control, so that a series of biochemical reactions are automatically completed. These valves generally fall into three broad categories: 1. the gas circuit is arranged on the lower layer of the fluid channel and integrated on the microfluidic chip, and the control channel is pressed by air pressure to expand, so that the upper fluid channel is closed. 2. Or a precise valve mechanical structure is designed to control liquid outside the microfluidic chip, and the structure is matched with the microfluidic flow channel to realize the automation of the reaction. 3. The valve is opened by applying solidified wax or such material to the chip to form a valve blocking fluid and melting the material by heating. Some of the above valve methods have their own challenges, and method 1 depends on flexible materials, and requires complicated air paths and interfaces for control, and the engineering realization stability challenge is large. Some mechanical structures of the method 2 require high processing difficulty, some rotating physical structures bring the risk of air tightness, and the problem of cross contamination among fluids is also prominent. In the method 3, the valve can not be closed again after being opened once, and the application is limited.

Therefore, at present, it is urgently needed to design a reaction device capable of effectively controlling liquid reaction, so as to improve experimental efficiency.

Disclosure of Invention

1. Problems to be solved

Aiming at the problems that the reaction liquid is inconvenient to control and the experiment efficiency and the accuracy are reduced in the prior art, the invention provides a reaction device capable of automatically controlling the liquid; through the cooperation between design column valve, uide bushing and the elastic hose, can effectively block or circulate reaction tube and liquid storage tube to solve the inconvenient, the problem that leads to experimental efficiency and precision to descend of reaction liquid control.

2. Technical scheme

In order to solve the problems, the technical scheme adopted by the invention is as follows:

the reaction device capable of automatically controlling liquid comprises a reaction tube and a plurality of liquid storage tubes, wherein the reaction tube is communicated with each liquid storage tube through an elastic hose; the columnar valve sleeve is arranged in the guide sleeve and is in sliding connection with the guide sleeve, and an elastic part is arranged between the columnar valve and the guide sleeve; the cylindrical valve is provided with a through hole matched with the elastic hose, and the elastic hose penetrates through the through hole; one end of the guide sleeve, which is close to the elastic hose, is provided with an extrusion end face; the elastic hose can be extruded on the extrusion end face through the telescopic columnar valve, and a liquid channel in the elastic hose is closed or is communicated.

The reaction cavity in the reaction tube can also be a sample adding cavity, and the liquid storage cavity in the liquid storage tube stores reaction liquid in advance in a fixed volume mode. Quantitative samples are added into the sample adding cavity, the sample adding cavity can be connected with atmosphere in a default state or can be connected with positive pressure in a sealing mode, and the opening of the liquid storage cavity is connected with the positive pressure or the atmosphere.

Preferably, an arc-shaped surface and an extrusion plane are arranged on the circumferential direction of the through hole; the arc-shaped surface is matched with the elastic hose; the extrusion plane extends from the arc surface to the outer side of the columnar valve, and the extrusion plane and the extrusion end surface are arranged in opposite directions.

Preferably, the extrusion plane is provided with an opening extending towards the outer side of the cylindrical valve.

Preferably, the area of the extrusion plane is A, the cross-sectional area of the cylindrical valve is B, and A/B is 0.3-0.8.

Preferably, the liquid storage pipe comprises a first liquid storage pipe and a second liquid storage pipe; the first liquid outlet end of the first liquid storage pipe is communicated with one end of a first elastic hose, and the other end of the first elastic hose is communicated with the reaction pipe; and the second liquid outlet end of the second liquid storage pipe is communicated with one end of a second elastic hose, and the other end of the second elastic hose is communicated with the reaction pipe.

Preferably, one end of the reaction tube is communicated with a four-way connecting tube, a port I of the four-way connecting tube is communicated with the first elastic hose, a port II of the four-way connecting tube is communicated with the second elastic hose, and a port III of the four-way connecting tube is communicated to the liquid flow channel of the transparent detection sheet.

Preferably, a shell is arranged outside the device, and the shell comprises a reaction area and a detection area; the reaction tube, the liquid storage tube and the guide sleeve are arranged on the shell of the reaction zone, and the columnar valve extends out of the reaction zone from the inside of the reaction zone; the transparent detection piece extends from the reaction area to be arranged in the detection area.

Preferably, a mounting plate is arranged at the top of the shell of the reaction area, and the reaction tube and the liquid storage tube are mounted on the mounting plate.

Preferably, the top of the shell of the detection area is provided with a detection port, and the detection port is positioned above the transparent detection sheet and used for observing a reaction liquid product in the transparent detection sheet.

Preferably, the transparent detection piece comprises an upper detection piece and a lower detection piece, the liquid flow channel is arranged on the lower detection piece, the flow channel inlet of the liquid flow channel is communicated with the port III, and the flow channel outlet of the liquid flow channel is communicated to the outside of the shell through a connecting valve.

Preferably, the elastic hose comprises an elastic silicone tube.

Preferably, the elastic member comprises an elastic silicone cover; the elastic silica gel cover is arranged at the top of the device, the guide sleeve is fixedly arranged in the device, and the columnar valve is connected with the guide sleeve through the elastic silica gel cover.

3. Advantageous effects

Compared with the prior art, the invention has the beneficial effects that:

(1) according to the reaction device capable of automatically controlling the liquid, disclosed by the invention, the elastic hose can be effectively opened or closed by telescopically controlling the simple columnar valve, so that the liquid storage pipe and the reaction pipe are communicated or disconnected, the flow of the reaction liquid can be effectively controlled, and the accuracy of the using amount of the reaction liquid and the experimental efficiency are effectively improved.

(2) The reaction device capable of automatically controlling the liquid has the advantages of simple integral structure and lower engineering difficulty, and realizes automatic reaction and detection with high reliability and low cost.

Drawings

FIG. 1 is a schematic view of a reaction apparatus (hidden housing) for automatically controlling liquid in accordance with the present invention in a closed state in an initial state;

FIG. 2 is a schematic view of the reaction device (with the shell hidden) for automatically controlling the liquid according to the present invention in an open state;

FIG. 3 is a side view of the FIG. 2 condition of the present invention;

FIG. 4 is a longitudinal cross-sectional view of the invention at the junction valve of FIG. 3;

FIG. 5 is a schematic view of the mating connection of the spool valve of the present invention with a guide sleeve;

FIG. 6 is a side view of a cylindrical valve of the present invention;

FIG. 7 is a perspective view of a reaction apparatus (with the housing removed) for automatically controlling a liquid according to the present invention;

FIG. 8 is a perspective view of the reaction apparatus of FIG. 7 with the detection sheet hidden;

FIG. 9 is a schematic view showing the connection between a liquid storage tube and a reaction tube according to the present invention;

FIG. 10 is a perspective view of a reaction apparatus for automatically controlling a liquid according to the present invention;

FIG. 11 is a perspective view II of a reaction apparatus for automatically controlling a liquid according to the present invention.

In the figure:

100. an elastic silica gel cover; 101. a first connection port; 102. a second connection port; 103. a sample addition port;

200. mounting a plate;

310. a first liquid storage tube; 311. a first liquid outlet end; 312. a first reservoir chamber; 320. a second liquid storage tube; 321. a second liquid outlet end; 322. a second reservoir chamber;

400. a reaction tube; 401. a reaction chamber; 410. a four-way connecting pipe; 411. a port I; 412. a port II; 413. port III; 420. an adapter;

500. an elastic hose; 510. a liquid channel;

600. a cylindrical valve; 601. a through hole; 602. an arc-shaped surface; 603. extruding a plane; 610. a guide sleeve; 611. extruding the end face;

710. a detection sheet is arranged; 720. a lower detection sheet; 721. a liquid flow passage; 722. a flow channel outlet; 730. a connecting valve; 731. a negative pressure driving interface;

800. a housing; 810. a reaction zone; 820. a detection zone; 821. and a detection port.

Detailed Description

The following detailed description of exemplary embodiments of the invention refers to the accompanying drawings, which form a part hereof, and in which are shown by way of illustration exemplary embodiments in which the invention may be practiced, and in which features of the invention are identified by reference numerals. The following more detailed description of the embodiments of the invention is not intended to limit the scope of the invention, as claimed, but is presented for purposes of illustration only and not limitation to describe the features and characteristics of the invention, to set forth the best mode of carrying out the invention, and to sufficiently enable one skilled in the art to practice the invention. It will, however, be understood that various modifications and changes may be made without departing from the scope of the invention as defined in the appended claims. The detailed description and drawings are to be regarded in an illustrative rather than a restrictive sense, and any such modifications and variations, if any, are intended to fall within the scope of the invention as described herein. Furthermore, the background is intended to be illustrative of the state of the art as developed and the meaning of the present technology and is not intended to limit the scope of the invention or the application and field of application of the invention.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs; the terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.

It will be understood that when an element is referred to as being "on" another element, it can be directly on the other element or intervening elements may also be present; when an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present; the terms "first," "second," "top," "bottom," and the like as used herein are for illustrative purposes only.

The invention is further described with reference to specific examples.

Examples

The embodiment provides a reaction device capable of automatically controlling liquid, which is mainly used for realizing the mixing reaction of a plurality of reaction components and effectively controlling the reaction process. Referring to fig. 1, 2 and 9, the device comprises a reaction tube 400 and a plurality of liquid storage tubes, wherein the reaction tube 400 is communicated with each liquid storage tube through a pipeline, so that each reaction component is only required to be put into the liquid storage tube, and the reaction components in the liquid storage tubes are distributed into the reaction tube 400 during an experiment, so that various biochemical and biological reactions are realized.

However, the existing problems are that the reaction components from the liquid storage tube to the reaction tube 400 are not easy to be effectively controlled, the leakage of the reaction liquid or the failure of the reaction liquid to pass through the reaction tube is easy to occur, which directly results in the decrease of the precision of the used amount of the reactant and the decrease of the experimental efficiency, and is very inconvenient in the actual operation.

Therefore, aiming at the above problems, the present embodiment improves the reaction apparatus, specifically, the reaction tube 400 is communicated with each liquid storage tube through the elastic hose 500, and the present embodiment can select an elastic silicone tube. In addition, a cylindrical valve 600 and a guide sleeve 610 are arranged in the device, the cylindrical valve 600 is sleeved in the guide sleeve 610 and is in sliding connection with the guide sleeve 610, an elastic part is arranged between the cylindrical valve 600 and the guide sleeve 610, a through hole 601 matched with the elastic hose 500 is arranged on the cylindrical valve 600, and the elastic hose 500 is arranged in the through hole 601 in a penetrating mode. In other embodiments, in order to further improve the telescopic stability of the cylindrical valve 600, the other end of the cylindrical valve 600 may be sleeved with the guide sleeve 610, and the guide sleeve 610 is mounted on the top of the device. Since the elastic tube 500 is easily deformed by compression, the elastic tube 500 can be compressed on the compression end surface 611 by the telescopic cylindrical valve 600 and the liquid channel 510 in the elastic tube 500 is closed, as shown in fig. 1, which is an initial closed state, and the reactant components are not mixed and reacted with each other; if it is desired to start the reaction, the telescopic cylinder valve 600 releases the squeezing state of the flexible tube 500, and the liquid channel 510 inside the flexible tube 500 is changed to a flow state due to the easy rebound of the flexible tube 500, as shown in fig. 2, so that each reaction component can flow into the reaction tube 400 to participate in the reaction, which is an open state.

Further, in order to more efficiently open or close the cylindrical valve 600, the through hole 601 may be further modified in some embodiments: an arc surface 602 and a pressing plane 603 are arranged on the circumference of the through hole 601, the arc surface 602 matches with the shape of the elastic hose 500, the pressing plane 603 extends horizontally from the arc surface 602 to the outside of the cylindrical valve 600, and the pressing plane 603 and the pressing end surface 611 are arranged opposite and parallel. Taking fig. 3 and fig. 6 as an example, when the cylindrical valve 600 moves downward relative to the guide sleeve 610, due to the arrangement of the arc-shaped surface 602 on the through hole 601, the through hole 601 will generate a tangential force to the elastic hose 500 to press the elastic hose 500 to the lower side of the pressing plane 603, so as to press the elastic hose 500 between the pressing plane 603 and the pressing end surface 611 flatly, thereby ensuring that the liquid passage 510 is completely closed (fig. 1), and improving the sealing performance in the closed state. When the cylindrical valve 600 moves upward relative to the guide sleeve 610, the elastic hose 500 will be elastically returned to the state of matching with the arc surface 602 due to its own elastic force, so as to ensure that the liquid passage 510 is completely opened (fig. 2). In summary, this can achieve effective opening or closing of the liquid channel 510, and the specific flow rate can be controlled by selecting a suitable position between the two states, thereby improving the experimental efficiency and accuracy.

In order to provide a sufficient pressing space, an opening may be provided in the pressing plane 603 to extend to the outside of the cylindrical valve 600. The applicant finds that, when the area of the pressing plane 603 is a, the cross-sectional area of the cylindrical valve 600 is B, and the a/B is set to 0.3-0.8, the sufficient pressing plane 603 can be provided, the requirement on sealing performance can be met, the matching performance of the elastic hose 500 and the arc-shaped surface 602 can be ensured, and the requirement on flow rate can be met.

It should be noted that, the above concept of the present invention can realize the liquid control among a plurality of liquid storage pipes, and the specific number of the liquid storage pipes is not limited. As a specific embodiment, two liquid storage tubes are used for illustration in this embodiment.

More specifically, the reservoir comprises a first reservoir 310 and a second reservoir 320, both of which are mounted on the mounting plate 200 at the top of the apparatus, as shown in fig. 7 to 9, the first reservoir 312 of the first reservoir 310 is connected to the first connection port 101 on the mounting plate 200, the second reservoir 322 of the second reservoir 320 is connected to the second connection port 102, and the first connection port 101 and the second connection port 102 can be connected to positive pressure or atmosphere to drive the liquid into the reaction tube 400; similarly, the reaction tube 400 is also mounted on the mounting plate 200, and the reaction chamber 401 of the reaction tube 400 is communicated to the sample addition port 103 on the mounting plate 200, so that reaction components can be put in or pressure driving can be performed through the connection port and the sample addition port 103. Referring to fig. 1 and 2, the first liquid outlet 311 of the first liquid storage tube 310 is connected to one end of a first flexible tube, the other end of the first flexible tube is connected to the reaction tube 400, the second liquid outlet 321 of the second liquid storage tube 320 is connected to one end of a second flexible tube, and the other end of the second flexible tube is connected to the reaction tube 400. The bottom end of the reaction tube 400 is communicated with a four-way connecting tube 410, a port I411 of the four-way connecting tube 410 is communicated with a first elastic hose, a port II 412 of the four-way connecting tube 410 is communicated with a second elastic hose, a port III 413 of the four-way connecting tube 410 is communicated to a liquid flow passage 721 of the transparent detection sheet through a joint 420, and a liquid product in the reaction tube 400 can be observed after flowing into the liquid flow passage 721.

In order to facilitate the detection of the reaction product, in this embodiment, the transparent detection sheet extends horizontally from the adapter 420, and an experimenter can observe the reaction liquid product in the liquid flow channel 721 in the transparent detection sheet in a plan view. The reaction apparatus of this embodiment includes a reaction region 810 and a detection region 820, wherein the aforementioned reaction tube 400, reservoir tube and guide sleeve 610 are installed in the housing 800 of the reaction region 810, and the cylindrical valve 600 extends from the interior of the reaction region 810 to the bottom of the housing 800 of the reaction region 810 (fig. 11). The transparent detection sheet is extended from the reaction area 810 to be arranged in the shell 800 of the detection area 820, the top of the shell 800 of the detection area 820 is provided with a detection port 821, the detection port 821 is positioned above the transparent detection sheet, and an experimenter can observe a reaction liquid product in the transparent detection sheet through the detection port 821.

In some embodiments, as shown in fig. 3 to 4 and fig. 7 to 8, the transparent detection sheet may be divided into an upper detection sheet 710 and a lower detection sheet 720, the liquid channel 721 is disposed on the lower detection sheet 720, a channel inlet of the liquid channel 721 is communicated with the adapter 420, a channel outlet 722 of the liquid channel 721 is communicated to the outside of the housing 800 through a connection valve 730, and the connection valve 730 is provided with a negative pressure driving interface 731 connected to the channel outlet 722 for externally connecting a negative pressure driving mechanism, so as to suck a reaction liquid product from the reaction tube 400 into the liquid channel 721 for observation under negative pressure.

In some embodiments, the elastic member may be an elastic silicone cover 100, the elastic silicone cover 100 is mounted on the top of the mounting plate 200, a connection port on the mounting plate 200 and the sample addition port 103 are correspondingly extended and disposed on the elastic silicone cover 100, the top of the cylindrical valve 600 passes through the mounting plate 200 and is connected with the elastic silicone cover 100, and the elastic silicone cover 100 at other positions is relatively fixedly disposed with the mounting plate 200. Therefore, in a natural state, the elastic silicone cap 100 may press the cylinder valve 600 downward (fig. 1) by its elastic force, which is greater than the upward repulsive force of the elastic hose 500; when it is desired to pass liquid through the liquid passage 510, the experimenter holds the cylindrical valve 600 extending from the bottom of the device and provides an upward force, and the elastic silicone cap 100 at the cylindrical valve 600 is lifted up (fig. 2 and 3), and the liquid passage 510 is opened.

In summary, the present invention designs the liquid path control structure of the reaction area 810 and the micro-fluidic chip structure (i.e. the transparent detection sheet) of the detection area 820, and the simple and easy column valve 600, the elastic hose 500 and the liquid storage tube are arranged in the device, the column valve 600 adopts the simple and easy column valve to control the elastic silicone tube, and the connection and disconnection of the pipeline connecting the liquid storage tube and the reaction tube 400 are completed by the up-and-down motion of the column valve 600. The column valve 600 is controlled by a mechanism outside the device, an air path driving opening is designed above a liquid storage cavity of the device and can be controlled by air pressure, and a negative pressure driving mechanism at the bottom of the device is communicated to the connecting valve 730, so that the reaction device has a simple structure and is easy to realize, as shown in fig. 10 and 11, the technical scheme of the invention can be completed by only controlling or connecting a plurality of components outside the device, and the column valve 600 is a reliable low-cost liquid control device. And the device is whole card box structure, and the user can add the sample after the reaction chamber, carries out full-automatic reaction control and detection with this structure in putting into equipment, and is very convenient.

The invention has been described in detail hereinabove with reference to specific exemplary embodiments thereof. It will, however, be understood that various modifications and changes may be made without departing from the scope of the invention as defined in the appended claims. The detailed description and drawings are to be regarded in an illustrative rather than a restrictive sense, and any such modifications and variations, if any, are intended to fall within the scope of the invention as described herein. Furthermore, the background is intended to be illustrative of the state of the art as developed and the meaning of the present technology and is not intended to limit the scope of the invention or the application and field of application of the invention.

More specifically, although exemplary embodiments of the invention have been described herein, the invention is not limited to these embodiments, but includes any and all embodiments modified, omitted, combined, e.g., between various embodiments, adapted and/or substituted, as would be recognized by those skilled in the art from the foregoing detailed description. The limitations in the claims are to be interpreted broadly based the language employed in the claims and not limited to examples described in the foregoing detailed description or during the prosecution of the application, which examples are to be construed as non-exclusive. Any steps recited in any method or process claims may be executed in any order and are not limited to the order presented in the claims. The scope of the invention should, therefore, be determined only by the appended claims and their legal equivalents, rather than by the descriptions and examples given above.

Claims

1. A reaction device capable of automatically controlling liquid is characterized by comprising

The reaction tube (400) is communicated with each liquid storage tube through an elastic hose (500);

the device comprises a cylindrical valve (600) and a guide sleeve (610), wherein the cylindrical valve (600) is sleeved in the guide sleeve (610) and is in sliding connection with the guide sleeve (610), and an elastic part is arranged between the cylindrical valve (600) and the guide sleeve (610); a through hole (601) matched with the elastic hose (500) is formed in the columnar valve (600), and the elastic hose (500) penetrates through the through hole (601); one end of the guide sleeve (610) close to the elastic hose (500) is provided with an extrusion end surface (611);

the elastic hose (500) can be pressed against the pressing end surface (611) by the telescopic cylinder valve (600) and the liquid passage (510) in the elastic hose (500) is closed, or the liquid passage (510) in the elastic hose (500) is circulated.

2. The reaction device for automatically controlling liquid as claimed in claim 1, wherein the through hole (601) is circumferentially provided with an arc-shaped surface (602) and a pressing plane (603); the arc-shaped surface (602) is matched with the elastic hose (500); the extrusion plane (603) extends from the arc-shaped surface (602) to the outer side of the cylindrical valve (600), and the extrusion plane (603) and the extrusion end surface (611) are arranged oppositely.

3. The reaction device for automatically controlling liquid as claimed in claim 2, wherein the pressing plane (603) is provided with an opening extending to the outside of the cylindrical valve (600).

4. The reaction device for automatically controlling liquid as claimed in claim 2, wherein the area of the extrusion plane (603) is A, the cross-sectional area of the cylindrical valve (600) is B, and A/B is 0.3-0.8.

5. The reaction device for automated control of liquids as claimed in claim 1, wherein said reservoir comprises a first reservoir (310) and a second reservoir (320); the first liquid outlet end (311) of the first liquid storage pipe (310) is communicated with one end of a first elastic hose, and the other end of the first elastic hose is communicated with the reaction pipe (400); and a second liquid outlet end (321) of the second liquid storage pipe (320) is communicated with one end of a second elastic hose, and the other end of the second elastic hose is communicated with the reaction pipe (400).

6. The reaction device capable of automatically controlling liquid according to claim 5, wherein one end of the reaction tube (400) is connected to a four-way connection tube (410), a port I (411) of the four-way connection tube (410) is connected to the first flexible tube, a port II (412) of the four-way connection tube (410) is connected to the second flexible tube, and a port III (413) of the four-way connection tube (410) is connected to the liquid flow channel (721) of the transparent detection sheet.

7. The reaction device for automatically controlling the liquid as claimed in claim 6, wherein a housing (800) is further provided outside the device, the housing (800) comprises a reaction zone (810) and a detection zone (820); the reaction tube (400), the liquid storage tube and the guide sleeve (610) are arranged on a shell (800) of the reaction area (810), and the cylindrical valve (600) extends out of the reaction area (810) from the inside of the reaction area (810); the transparent detection piece extends from the reaction area (810) to be arranged in the detection area (820).

8. The reaction device for automatically controlling liquid as claimed in claim 7, wherein a mounting plate (200) is provided on the top of the housing (800) of the reaction zone (810), and the reaction tube (400) and the reservoir tube are mounted on the mounting plate (200).

9. The reaction device for automatically controlling liquid according to claim 7, wherein the detection area (820) is provided with a detection port (821) at the top of the housing (800), and the detection port (821) is positioned above the transparent detection sheet for observing the reaction liquid product in the transparent detection sheet.

10. The reaction device for automatically controlling liquid according to claim 7, wherein the transparent detection sheet comprises an upper detection sheet (710) and a lower detection sheet (720), the liquid flow channel (721) is disposed on the lower detection sheet (720), the flow channel inlet of the liquid flow channel (721) is connected to the port III (413), and the flow channel outlet (722) of the liquid flow channel (721) is connected to the outside of the housing (800) through the connection valve (730).

11. The reaction device for automatically controlling liquid according to any one of claims 1 to 10, wherein the flexible tube (500) comprises a flexible silicone tube.

12. The reaction device for automatically controlling liquid according to any one of claims 1 to 10, wherein the elastic member comprises an elastic silicone cover (100); the elastic silica gel cover (100) is arranged at the top of the device, the guide sleeve (610) is fixedly arranged in the device, and the cylindrical valve (600) is connected with the guide sleeve (610) through the elastic silica gel cover (100).