CN114617256A - A health promotion formula containing SOD and 5-HTP for resisting oxidation, relieving fatigue and improving sleep - Google Patents
A health promotion formula containing SOD and 5-HTP for resisting oxidation, relieving fatigue and improving sleep Download PDFInfo
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- CN114617256A CN114617256A CN202210244996.5A CN202210244996A CN114617256A CN 114617256 A CN114617256 A CN 114617256A CN 202210244996 A CN202210244996 A CN 202210244996A CN 114617256 A CN114617256 A CN 114617256A
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/15—Vitamins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/15—Vitamins
- A23L33/155—Vitamins A or D
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/175—Amino acids
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention discloses a health-care formula containing SOD and 5-HTP for resisting oxidation, resisting fatigue and improving sleep, belonging to the technical field of health-care products. The health care formula comprises: 10-20 parts of SOD, 5-HTP10-25 parts of magnesium malate, 0.01-0.1 part of vitamin and 10-20 parts of auxiliary materials. The above materials are used as main materials to prepare various health foods in different dosage forms, such as tablet, pill, powder, hard capsule, soft capsule, granule, oral liquid, beverage, candy, biscuit, etc. The invention can obviously enhance the oxidation resistance and eliminate excessive oxygen free radicals through the synergistic action between the SOD and the 5-HTP, and simultaneously plays a certain role in improving sleep. The health formula prepared by the raw materials of magnesium malate, vitamins and the like has good effects of resisting oxidation and fatigue and improving the sleep quality. After long-term administration, the health-care food can effectively relieve the fatigue of the organism, improve the sleep quality and improve the disease defense capability of the human body.
Description
Technical Field
The invention belongs to the technical field of health care products, and particularly relates to a health care formula containing SOD and 5-HTP for resisting oxidation, resisting fatigue and improving sleep.
Background
Sleep is the most basic physiological process of the human body, and approximately one third of the life of a person is spent asleep. Sufficient and high-quality sleep is closely related to physical and mental health, safety, life quality and the like. With the increasing pace of life and the increasing pressure of life and work, sleep diseases such as insomnia have become a worldwide public health problem affecting human health. Epidemiological data show that about 10 to 15 percent of natural people in China suffer from primary insomnia, wherein the primary insomnia mainly manifests as difficulty in falling asleep, insufficient sleep, easy awakening, dreaminess and the like. According to the 2018 Chinese sleep quality survey report, over 16% of respondents have insufficient sleep at night, and 25.83% of respondents have difficulty falling asleep. Also, many studies have shown that sleep-related diseases are associated with various other diseases such as depression, endocrine diseases, alzheimer's disease, etc.; meanwhile, the problem of insufficient sleep can cause the symptoms of frequent fatigue, poor spirit, physical strength, "overdraw", low immunity, susceptibility to cold, accelerated natural aging and the like. It is found that the free radicals in the human body are in dynamic equilibrium under normal conditions, but the excessive or slow removal of the free radicals can cause a series of damages to the organism, accelerate the aging of the human body and induce various symptoms, such as fatigue and the like. The sleep problems such as insufficient sleep and the like can cause the human body to feel fatigue, and simultaneously, the free radical balance of the human body can be induced to change, so that the health of the human body is seriously affected. Some health foods have been disclosed in view of the above circumstances, but the conventional health foods have problems:
1. the health care product has various names and functions, has the beautifying functions of oxidation resistance and the like, has the anti-fatigue function and the sleep improving function, but almost does not have the health care product which integrates the functions of oxidation resistance, fatigue resistance, sleep improvement and the like;
various antioxidant components such as SOD and the like are easy to be volatile and active in high-temperature and slightly acidic and slightly alkaline environments;
3. the existing preparation process is too simple, only various components are directly mixed, mutual compatibility in the process is not available, and related components cannot play a better role in the preparation process in vivo;
therefore, the development of a health-care formula which is efficient and stable, has the functions of resisting oxidation and fatigue and improving sleep is urgently needed.
Disclosure of Invention
[ problem ] to
The existing health food cannot have multiple physiological functions of resisting oxidation, resisting fatigue, improving sleep and the like, and the existing health food for improving sleep cannot effectively resist fatigue or effectively remove free radicals.
[ solution ]
Aiming at the problems, the invention provides an anti-oxidation, anti-fatigue and sleep-improving health formula containing SOD and 5-HTP, and the health formula has multiple functions of resisting oxidation, resisting fatigue, improving sleep and the like, can effectively remove free radicals, relieve fatigue and improve sleep quality.
In order to achieve the purpose, the invention provides the following technical scheme:
a health-care formula containing SOD and 5-HTP for resisting oxidation, resisting fatigue and improving sleep comprises, by weight, 10-20 parts of superoxide dismutase (SOD, the same below), 10-25 parts of 5-hydroxytryptophan (5-HTP, the same below), 5-10 parts of magnesium malate, 0.01-0.1 part of vitamin and 10-20 parts of auxiliary materials.
In one embodiment of the present invention, the unit enzyme activity of superoxide dismutase (SOD) is 10000-50000 IU/g.
In one embodiment of the invention, the SOD is a stable SOD prepared by kexin yangzhen biotechnology limited in Shenzhen, see CN 108018266A.
In one embodiment of the invention, the vitamin includes one, several or all of vitamin a, vitamin D, vitamin E, vitamin C, vitamin B1, vitamin B2, vitamin B6, vitamin B12, niacin, folic acid, pantothenic acid, choline and nicotinamide.
In one embodiment of the present invention, the health formulation preferably comprises, in parts by weight: SOD20 parts, 5-HTP25 parts, magnesium malate 10 parts, vitamins 0.07 part (vitamin A0.7mg, vitamin E8 mg, vitamin C36 mg, vitamin D0.2 mg, vitamins, B10.6mg, vitamins B21.6mg, vitamins B61.6mg, vitamin B125 mg, nicotinic acid 0.9mg, folic acid 0.7mg, pantothenic acid 4mg, choline 43mg, nicotinamide 0.6mg), and adjuvants 10-20 parts.
In one embodiment of the invention, the auxiliary materials comprise one, several or all of a filling agent, a flavoring agent, a glidant, a preservative, a binder and a disintegrating agent.
In one embodiment of the invention, the filler is one, several or all of starch, dextrin and lactose; the correctant is one, more or all of Mel, simple syrup, pericarpium Citri Junoris syrup, fructus Pruni Pseudocerasi syrup and cortex Cinnamomi Japonici syrup; the glidant is one, more or all of talcum powder, micro-powder silica gel and silicon dioxide; the antiseptic is one, several or all of benzoic acid and its sodium salt, p-hydroxybenzoate and its sodium salt, sorbic acid and its potassium salt, and sodium acetate; the adhesive is one, several or all of starch slurry, microcrystalline cellulose, gelatin and polyvinyl alcohol; the disintegrant is one, several or all of dry starch, sodium carboxymethyl starch (CMS-Na), low-substituted hydroxypropyl cellulose (L-HPC), croscarmellose sodium (CCMC-Na), and crospovidone (PVPP).
In the embodiment of the invention, the health formula can be prepared into tablets, pills, powder, hard capsules, soft capsules, granules, oral liquid, beverages, candies, biscuits or other food-acceptable dosage forms.
The invention also provides a preparation method of the health-care formula, which comprises the following steps:
(1) weighing SOD, 5-HTP, magnesium malate, vitamins and auxiliary materials in a clean container according to the proportion;
(2) mixing and stirring 10-20 parts of SOD, 5-10 parts of magnesium malate and 20-60% of pure water by volume at the rotation speed of 600-800rpm and the temperature of 50-60 ℃, and cooling to 37 ℃ after stirring uniformly;
(3) slowly adding 5-HTP10-25 parts and 0.01-0.1 part of vitamin into the solution in the step (2) for multiple times, gradually adding 25-50% pure water by volume while stirring, and maintaining the conditions in the step (2) until the mixture is uniformly stirred;
(4) and finally, adding 10-20 parts of auxiliary materials and 10% -50% of pure water by volume, completely and uniformly stirring to obtain the required solution, and preparing into corresponding dosage forms for sterile subpackaging.
In an embodiment of the present invention, the aseptic dispensing in step (2) is sterilization using ultraviolet irradiation.
The invention also provides a health food containing the health formula and the like.
Compared with the existing formula, the invention has the beneficial effects that:
SOD has good antioxidant effect, 5-HTP can obviously improve sleep quality and reduce fatigue feeling, and the synergistic effect of the SOD and the HTP can obviously enhance the antioxidant capacity and eliminate excessive oxygen free radicals and can also play a role in improving the sleep quality.
2. Meanwhile, the magnesium malate can be compounded with part of the vitamins, has a synergistic effect and can play a role in promoting the synthesis of protein and enzyme; meanwhile, the magnesium malate has the function of relieving the symptoms of chronic fatigue syndrome and can effectively relieve fatigue.
3. The general product for improving sleep only uses 5-HTP which is an active ingredient, and the formula also directly supplements SOD enzyme on the basis of the effective ingredient, can effectively remove free radicals and relieve fatigue caused by insufficient sleep.
4. The traditional SOD is unstable and is easy to inactivate under the conditions of high temperature or partial acid to partial alkali, and the invention adopts the Zhongkexin super-stable SOD yeast powder which is difficult to inactivate in vivo and can better play the role; and many antioxidants are very unstable, need strict anti-oxidation operation in the preparation process, and the shelf life of the product is short, and cannot exist in the form of solution, while the zhongkouxiang SOD is very stable and has very strong anti-oxidation activity, so that the product can exist in various forms, and the shelf life of the product is prolonged.
5. Different from other formulas, the formula also improves the corresponding preparation process, so that the formula has better compatibility effect and use value.
Drawings
FIG. 1 shows experimental results of superoxide anion removal rates of powders of examples 1 to 5 and comparative examples 1 to 8.
FIG. 2 shows the results of experiments on the stability of enzyme activity of powders of examples 1 to 5 and comparative examples 1 to 8.
Detailed Description
The present invention is further described below with reference to examples, but the embodiments of the present invention are not limited thereto.
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
The invention provides an anti-oxidation, anti-fatigue and sleep-improving health-care formula containing SOD and 5-HTP, which comprises the following raw materials, by weight, 10-20 parts of SOD, 10-25 parts of 5-HTP, 5-10 parts of magnesium malate, 0.01-0.1 part of vitamin and 10-20 parts of auxiliary materials.
The vital function cells of the human body are composed of enzymes, the enzymes are organic matters composed of proteins and some chemical elements, the human and even the earth organisms can not survive after leaving the enzymes, the qi and blood balance decrypted by the traditional Chinese medicine is actually the balance of the enzymes and free radicals, the free radicals are metabolites of the organisms and have biological activity and different functions, many free radicals are guardians of the human life, the superoxide anion free radicals can be attached to the cells and the inside and outside of the human body and the cells and the organs, the superoxide anion free radicals on the cells and the organs are garbage, the accumulated garbage can cause the cells to lose the due functions, and the superoxide anion free radicals, such as rusty spots on iron ware, can destroy the metabolism of the cells and cause the death of the cells. SOD is an enzyme that is focused on eliminating superoxide anion free radicals, and converts superoxide anion free radicals into water and oxygen to be discharged out of the body, so that the cell function can be recovered, and the cell aging can be prevented. Supplementing exogenous SOD can effectively improve the oxidation resistance of the organism and delay the aging of the organism, thereby effectively relieving the fatigue of the organism. Wherein, the SOD used in the invention is the hyperstable SOD with the activity of 10000-50000 IU/g.
5-HTP is a natural amino acid not involved in protein synthesis. In mammals, 5-HTP is the precursor of the neurotransmitter serotonin and melatonin, has a regulating effect on physiological functions such as sleep, pain, body temperature, appetite and behavior, and has been successfully used for treating diseases such as depression, insomnia and migraine due to its biological and pharmacological properties and its advantages such as safety and stability.
Magnesium malate (Magnesium malate) is a nutritional supplement whose components mainly comprise Magnesium and malic acid, both of which have great benefits for cell production and energy storage. The magnesium in the vitamin complex can also generate a synergistic effect with part of vitamins, and is better helpful for proper functions of proteins and amino acids. And some nutritionists and doctors believe that magnesium malate helps alleviate the symptoms of chronic fatigue syndrome. Chronic fatigue syndrome is characterized by sudden onset of fatigue, which can lead people to be weak and powerless throughout the day. In addition, it helps the body to produce Adenosine Triphosphate (ATP), an energy substance that allows cells to store energy and provide it when needed. It is produced at the mitochondria and is utilized by every cell. Therefore, it is very important for the normal function of the body.
The vitamins are essential nutrients for human body, can supplement vitamins required by human body, and have protection and synergistic effect on other components. For example, vitamin B6 is involved in protein synthesis and decomposition, amino acid metabolism, and nucleic acid and DNA synthesis, while vitamin B6-dependent enzymes play an important role in the synthesis of 5 important neurotransmitters: 5-hydroxytryptamine, dopamine, epinephrine, norepinephrine, and gamma-aminobutyric acid.
Example 1
The invention relates to an anti-oxidation, anti-fatigue and sleep-improving health formula containing SOD and 5-HTP, wherein the contents of the components are as follows: 15 parts of SOD, 10 parts of 5-HTP, 5 parts of magnesium malate, 0.06 part of vitamin (vitamin A0.5mg, vitamin E5 mg, vitamin C35 mg, vitamin D0.2 mg, vitamin, B10.6mg, vitamin B21.6mg, vitamin B61.6mg, vitamin B120 mg, nicotinic acid 0.9mg, folic acid 0.7mg, pantothenic acid 4mg, choline 45mg and nicotinamide 0.6mg), 20 parts of auxiliary material (honey 20 parts) and 49.94 parts of pure water.
(1) Mixing and stirring 15 parts of SOD, 5 parts of magnesium malate and 20% pure water at the rotating speed of 600rpm and the temperature of 50 ℃, and cooling to 37 ℃ after stirring uniformly;
(2) slowly adding 10 parts of 5-HTP and 0.06 part of vitamin (vitamin A0.5mg, vitamin E5 mg, vitamin C35 mg, vitamin D0.2 mg, vitamin, B10.6mg, vitamin B21.6mg, vitamin B61.6mg, vitamin B120 mg, nicotinic acid 0.9mg, folic acid 0.7mg, pantothenic acid 4mg, choline 45mg and nicotinamide 0.6mg) into the solution in the step (1) for multiple times, gradually adding 40% of pure water by volume while stirring, and maintaining the conditions in the step (1) until stirring uniformly;
(3) and finally, adding 20 parts of auxiliary materials (20 parts of honey)) and 40% pure water by volume, completely and uniformly stirring to obtain the required solution, preparing the oral liquid, and performing sterile subpackaging.
Example 2
The invention relates to an anti-oxidation, anti-fatigue and sleep-improving health formula containing SOD and 5-HTP, wherein the contents of the components are as follows: SOD20 parts, 5-HTP25 parts, magnesium malate 10 parts, vitamins 0.07 part (vitamin A0.7mg, vitamin E8 mg, vitamin C36 mg, vitamin D0.2 mg, vitamins, B10.6mg, vitamins B21.6mg, vitamins B61.6mg, vitamin B125 mg, nicotinic acid 0.9mg, folic acid 0.7mg, pantothenic acid 4mg, choline 43mg, nicotinamide 0.6mg), adjuvants 10 parts (croscarmellose sodium 5 parts, dextrin 3 parts, silicon dioxide 1 part, slurry 1 part), and pure water 34.93 parts.
(1) Mixing and stirring evenly SOD20 parts, magnesium malate 10 parts and 40% volume pure water at the rotation speed of 600-800rpm and the temperature of 50-60 ℃, and cooling to 37 ℃ after even stirring;
(2) slowly adding 25 parts of 5-HTP and 0.07 part of vitamin (vitamin A0.7mg, vitamin E8 mg, vitamin C36 mg, vitamin D0.2 mg, vitamin, B10.6 mg, vitamin B21.6 mg, vitamin B61.6mg, vitamin B125 mg, nicotinic acid 0.9mg, folic acid 0.7mg, pantothenic acid 4mg, choline 43mg and nicotinamide 0.6mg) into the solution in the step (1) for multiple times, gradually adding 50% of pure water while stirring, and maintaining the conditions in the step (1) until stirring uniformly;
(3) and finally, adding 10 parts of auxiliary materials (5 parts of croscarmellose sodium, 3 parts of dextrin, 1 part of silicon dioxide and 1 part of starch slurry) and 10% pure water by volume, completely and uniformly stirring to obtain a required solution, tabletting, preparing tablets, and performing sterile subpackaging.
Example 3
The invention relates to an anti-oxidation, anti-fatigue and sleep-improving health formula containing SOD and 5-HTP, wherein the contents of the components are as follows: 18 parts of SOD, 18 parts of 5-HTP, 6 parts of magnesium malate, 0.08 part of vitamin (vitamin A0.7mg, vitamin E10mg, vitamin C38 mg, vitamin D0.2 mg, vitamin, B10.6 mg, vitamin B21.6 mg, vitamin B61.6mg, vitamin B130 mg, nicotinic acid 0.9mg, folic acid 0.7mg, pantothenic acid 4mg, choline 46mg and nicotinamide 0.6mg), 20 parts of auxiliary material (dextrin 13 parts, silicon dioxide 1 part, starch slurry 6 parts) and 37.92 parts of pure water.
(1) Mixing and stirring 18 parts of SOD, 6 parts of magnesium malate and 60% pure water at the rotating speed of 600rpm and the temperature of 50 ℃, and cooling to 37 ℃ after stirring uniformly;
(2) slowly adding 18 parts of 5-HTP and 0.08 part of vitamin (vitamin A0.7mg, vitamin E10mg, vitamin C38 mg, vitamin D0.2 mg, vitamin, B10.6 mg, vitamin B21.6 mg, vitamin B61.6mg, vitamin B130 mg, nicotinic acid 0.9mg, folic acid 0.7mg, pantothenic acid 4mg, choline 46mg and nicotinamide 0.6mg) into the solution in the step (1) for multiple times, gradually adding 30% of pure water by volume while stirring, and maintaining the conditions in the step (1) until stirring uniformly;
(3) and finally, adding 20 parts of auxiliary materials ((13 parts of dextrin, 1 part of silicon dioxide and 6 parts of starch slurry) and 10% pure water by volume, completely and uniformly stirring to obtain the required solution, preparing into capsules, and performing sterile subpackaging.
Example 4
The invention relates to a health care formula containing SOD and 5-HTP for resisting oxidation, resisting fatigue and improving sleep, wherein the contents of all components are as follows: 14 parts of SOD, 16 parts of 5-HTP, 8 parts of magnesium malate, 0.05 part of vitamin (vitamin A0.7mg, vitamin E9 mg, vitamin C36 mg, vitamin D0.2 mg, vitamin, B10.6 mg, vitamin B21.5mg, vitamin B61.5mg, vitamin B128 mg, nicotinic acid 0.9mg, folic acid 0.7mg, pantothenic acid 4mg, choline 47mg and nicotinamide 0.6mg), 20 parts of auxiliary material (dextrin 5 parts, starch 5 parts, glucose 5 parts, microcrystalline cellulose 5 parts) and 41.95 parts of pure water.
(1) Mixing and stirring 14 parts of SOD, 8 parts of magnesium malate and 30% pure water at the rotating speed of 700rpm and the temperature of 55 ℃, and cooling to 37 ℃ after stirring uniformly;
(2) slowly adding 16 parts of 5-HTP and 0.05 part of vitamin (vitamin A0.7mg, vitamin E9 mg, vitamin C36 mg, vitamin D0.2 mg, vitamin, B10.6mg, vitamin B21.5mg, vitamin B61.5mg, vitamin B128 mg, nicotinic acid 0.9mg, folic acid 0.7mg, pantothenic acid 4mg, choline 47mg and nicotinamide 0.6mg) into the solution in the step (1) for multiple times, gradually adding 50% of pure water while stirring, and maintaining the conditions in the step (1) until stirring is uniform;
(3) and finally, adding 20 parts of auxiliary materials (5 parts of dextrin, 5 parts of starch, 5 parts of glucose and 5 parts of microcrystalline cellulose) and 20% pure water by volume, completely and uniformly stirring to obtain a required solution, preparing into granules and performing sterile subpackaging.
Example 5
The invention relates to an antioxidant, anti-fatigue and sleep-improving health formula containing SOD and 5-HTP, wherein the contents of the components are as follows: 10 parts of SOD, 20 parts of 5-HTP, 7 parts of magnesium malate, 0.05 part of vitamin (vitamin A0.7mg, vitamin E8 mg, vitamin C35 mg, vitamin D0.2 mg, vitamin, B10.6 mg, vitamin B21.5 mg, vitamin B61.5mg, vitamin B125 mg, nicotinic acid 0.9mg, folic acid 0.7mg, pantothenic acid 4mg, choline 46mg and nicotinamide 0.6mg), and after uniformly mixing the components, 20 parts of auxiliary materials (dextrin 5, starch 10, honey 5) and 42.95 parts of pure water are added.
(1) Mixing and stirring 10 parts of SOD, 7 parts of magnesium malate and 25% pure water at the rotating speed of 800rpm and the temperature of 60 ℃, and cooling to 37 ℃ after stirring uniformly;
(2) slowly adding 5-HTP 20 parts and vitamin 0.05 parts (vitamin A0.7mg, vitamin E8 mg, vitamin C35 mg, vitamin D0.2 mg, vitamin, B10.6mg, vitamin B21.5mg, vitamin B61.5mg, vitamin B125 mg, nicotinic acid 0.9mg, folic acid 0.7mg, pantothenic acid 4mg, choline 46mg and nicotinamide 0.6mg) into the solution in the step (1) for multiple times, gradually adding 25% of pure water while stirring, and maintaining the conditions in the step (1) until stirring uniformly;
(3) and finally, adding 20 parts of auxiliary materials (dextrin 5, starch 10 and honey 5) and 50% pure water by volume, completely and uniformly stirring to obtain the required solution, preparing pills and carrying out sterile subpackaging.
Comparative example 1 omitting SOD on the basis of example 2
The invention relates to an anti-oxidation, anti-fatigue and sleep-improving health formula containing SOD and 5-HTP, wherein the contents of the components are as follows: 5-HTP25 parts, magnesium malate 10 parts, vitamins 0.07 part (vitamin A0.7mg, vitamin E8 mg, vitamin C36 mg, vitamin D0.2 mg, vitamins, B10.6 mg, vitamin B21.6 mg, vitamins B61.6mg, vitamin B125 mg, nicotinic acid 0.9mg, folic acid 0.7mg, pantothenic acid 4mg, choline 43mg and nicotinamide 0.6mg), auxiliary materials 10 parts (croscarmellose sodium 5 parts, dextrin 3 parts, silicon dioxide 1 part, starch slurry 1 part) and pure water 54.93 parts.
(1) Mixing 10 parts of magnesium malate and 40% by volume of pure water at the rotation speed of 600-800rpm and the temperature of 50-60 ℃, uniformly stirring, and cooling to 37 ℃;
(2) slowly adding 25 parts of 5-HTP and 0.07 part of vitamin (vitamin A0.7mg, vitamin E8 mg, vitamin C36 mg, vitamin D0.2 mg, vitamin, B10.6 mg, vitamin B21.6 mg, vitamin B61.6mg, vitamin B125 mg, nicotinic acid 0.9mg, folic acid 0.7mg, pantothenic acid 4mg, choline 43mg and nicotinamide 0.6mg) into the solution in the step (1) for multiple times, gradually adding 50% of pure water while stirring, and maintaining the conditions in the step (1) until stirring uniformly;
(3) and finally, adding 10 parts of auxiliary materials (5 parts of croscarmellose sodium, 3 parts of dextrin, 1 part of silicon dioxide and 1 part of starch slurry) and 10% pure water by volume, completely stirring uniformly, tabletting, preparing tablets and performing aseptic subpackaging.
Comparative example 2 the 5-HTP omission was made on the basis of example 2
The invention relates to an anti-oxidation, anti-fatigue and sleep-improving health formula containing SOD and 5-HTP, wherein the contents of the components are as follows: 20 parts of SOD, 10 parts of magnesium malate, 0.07 part of vitamin (vitamin A0.7mg, vitamin E8 mg, 36mg of vitamin C, 0.2mg of vitamin D, vitamin, B10.6mg, vitamin B21.6mg, vitamin B61.6mg, vitamin B125 mg, 0.9mg of nicotinic acid, 0.7mg of folic acid, 4mg of pantothenic acid, 43mg of choline and 0.6mg of nicotinamide), 10 parts of auxiliary material (5 parts of croscarmellose sodium, 3 parts of dextrin, 1 part of silicon dioxide and 1 part of starch slurry) and 59.93 parts of pure water.
(1) Mixing and stirring evenly SOD20 parts, magnesium malate 10 parts and 40% volume pure water at the rotation speed of 600-800rpm and the temperature of 50-60 ℃, and cooling to 37 ℃ after even stirring;
(2) slowly adding 0.07 part of vitamin (vitamin A0.7mg, vitamin E8 mg, vitamin C36 mg, vitamin D0.2 mg, vitamin, B10.6mg, vitamin B21.6mg, vitamin B61.6mg, vitamin B125 mg, nicotinic acid 0.9mg, folic acid 0.7mg, pantothenic acid 4mg, choline 43mg and nicotinamide 0.6mg) into the solution in the step (1) for multiple times, gradually adding 50% of pure water in volume while stirring, and maintaining the conditions in the step (1) under the stirring condition until the stirring condition is uniform;
(3) and finally, adding 10 parts of auxiliary materials (5 parts of croscarmellose sodium, 3 parts of dextrin, 1 part of silicon dioxide and 1 part of starch slurry) and 10% pure water by volume, completely stirring uniformly, tabletting, preparing tablets and performing aseptic subpackaging.
Comparative example 3 magnesium malate was omitted from example 2
The invention relates to an anti-oxidation, anti-fatigue and sleep-improving health formula containing SOD and 5-HTP, wherein the contents of the components are as follows: SOD20 parts, 5-HTP25 parts, vitamin 0.07 part (vitamin A0.7mg, vitamin E8 mg, vitamin C36 mg, vitamin D0.2 mg, vitamin B10.6 mg, vitamin B21.6 mg, vitamin B61.6mg, vitamin B125 mg, nicotinic acid 0.9mg, folic acid 0.7mg, pantothenic acid 4mg, choline 43mg, nicotinamide 0.6mg), auxiliary material 10 parts (croscarmellose sodium 5 parts, dextrin 3 parts, silicon dioxide 1 part, starch slurry 1 part), and pure water 44.93 parts.
(1) Mixing and stirring evenly SOD20 parts and pure water with the volume of 40% at the rotating speed of 600-800rpm and the temperature of 50-60 ℃, and cooling to 37 ℃ after even stirring;
(2) slowly adding 25 parts of 5-HTP and 0.07 part of vitamin (vitamin A0.7mg, vitamin E8 mg, vitamin C36 mg, vitamin D0.2 mg, vitamin, B10.6 mg, vitamin B21.6 mg, vitamin B61.6mg, vitamin B125 mg, nicotinic acid 0.9mg, folic acid 0.7mg, pantothenic acid 4mg, choline 43mg and nicotinamide 0.6mg) into the solution in the step (1) for multiple times, gradually adding 50% of pure water while stirring, and maintaining the conditions in the step (1) until stirring uniformly;
(3) and finally, adding 10 parts of auxiliary materials (5 parts of croscarmellose sodium, 3 parts of dextrin, 1 part of silicon dioxide and 1 part of starch slurry) and 10% pure water by volume, completely stirring uniformly, tabletting, preparing tablets and performing aseptic subpackaging.
Comparative example 4 omission of vitamins from example 2
The invention relates to an anti-oxidation, anti-fatigue and sleep-improving health formula containing SOD and 5-HTP, wherein the contents of the components are as follows: SOD20 parts, 5-HTP25 parts, magnesium malate 10 parts, auxiliary materials 10 parts (croscarmellose sodium 5 parts, dextrin 3 parts, silicon dioxide 1 part, starch slurry 1 part), and pure water 35 parts.
(1) Mixing and stirring evenly SOD20 parts, magnesium malate 10 parts and 40% volume pure water at the rotation speed of 600-800rpm and the temperature of 50-60 ℃, and cooling to 37 ℃ after even stirring;
(2) slowly adding 25 parts of 5-HTP into the solution in the step (1) for multiple times, gradually adding 50% pure water by volume while stirring, and maintaining the conditions in the step (1) until stirring is uniform;
(3) and finally, adding 10 parts of auxiliary materials (5 parts of croscarmellose sodium, 3 parts of dextrin, 1 part of silicon dioxide and 1 part of starch slurry) and 10% pure water by volume, completely stirring uniformly, tabletting, preparing tablets and performing aseptic subpackaging.
Comparative example 5
The SOD of example 2 was replaced with glutathione, and the remaining composition and preparation process were the same as in example 2, to prepare the desired solution.
Comparative example 6
The procedure of example 2 was modified to directly mix SOD, 5-HTP, magnesium malate, vitamins, etc., and the remaining composition was the same as example 2 to prepare a desired solution.
Comparative example 7
The 5-HTP of example 2 was replaced with melatonin, and the remaining composition and preparation procedure were the same as in example 2, to prepare the desired solution.
Comparative example 8
When the SOD of example 2 was replaced with ordinary SOD (derived from pig blood extraction, enzyme activity 50000U), the remaining composition and preparation process were the same as example 2, and the desired solution was prepared.
Example 7
And (3) testing the oxidation resistance:
in the superoxide anion removal experiment, the required solution was prepared according to the kit instructions, and the reagents were added for reaction according to table 1 below.
TABLE 1 kit description
Defining: in the reaction system, the change value of the superoxide anion radical inhibited by the reaction at 37 ℃ for 40 minutes per liter of serum (slurry) is equivalent to 1 mg of the superoxide anion radical inhibited by vitamin C as one activity unit.
Calculating the formula:
the results of the superoxide anion scavenging experiments are shown in figure 1. As can be seen from the figure, the compounding of SOD and 5-HTP showed stronger inhibition of superoxide anion, wherein the superoxide anion scavenging rate of example 2 was the highest at 96.96%, while comparative example 1, in which SOD was not added, had almost no superoxide anion scavenging ability. In addition, the comparative example to which other antioxidant ingredients are added without the zhongkexin SOD ingredient shows a more general superoxide anion radical scavenging ability, for example, the scavenging rate of comparative example 5 is only 51.01%, which also shows that zhongkexin SOD has a stronger radical scavenging rate than the general antioxidant ingredient. The difference of the manufacturing process also affects the radical clearance of the product, and after the product is directly mixed and stirred uniformly, the radical clearance is only 60.66%, which also shows that the process of multiple batch slow addition adopted in the embodiment 2 has better effect.
Example 8
And (3) detecting enzyme activity stability:
the enzyme activity assay was carried out with reference to the modified Marklund method, namely GBT5009.171-2003 pyrogallol autoxidation. The analysis steps are as follows:
(1) determination of pyrogallol autoxidation rate
2.35mL of solution A, 2.00mL of distilled water, and 0.15mL of solution B were added to a 10mL cuvette at about 25 ℃. Adding the solution B, mixing uniformly, adding into a cuvette, and measuring the initial value at 325nm and the absorbance value after 1min, wherein the difference is the auto-oxidation rate delta A of pyrogallol325(min-1)。
(2) Determination of sample liquid and SOD enzyme liquid for inhibiting pyrogallol autoxidation rate
Adding a certain amount of sample liquid or enzyme liquid according to the step of measuring the auto-oxidation rate of pyrogallolThe autoxidation rate of the pyrogallol is inhibited to be about 1/2 delta A325(min-1) I.e. Δ A'325(min-1) Is 0.030.
SOD activity assay the loading order is given in the following table:
TABLE 2 SOD Activity test sample application sequence
The calculation formula is as follows:
(1) enzyme powder:
in the formula:
u/g: -a SOD enzyme activity unit;
△A325: -the pyrogallol autooxidation rate;
ΔA′325: -the sample solution or the SOD enzyme solution inhibits the rate of pyrogallol autoxidation;
v: the volume of enzyme solution or sample solution added in milliliters (mL);
d: -dilution of the enzyme solution or sample solution;
4.5- -total volume of reaction in milliliters (mL).
(2) Enzyme solution:
in the formula:
U/mL: -a SOD enzyme activity unit;
△A325: -pyrogallol autooxidation rate;
ΔA′325: the sample solution or the SOD enzyme solution inhibits the rate of pyrogallol autoxidation;
v: the volume of enzyme solution or sample solution added in milliliters (mL);
d: dilution of the enzyme solution or sample solution;
4.5- -total volume of reaction solution in milliliters (mL).
Note: (1) the absolute difference between two independent measurements obtained under repetitive conditions must not exceed 10% of the arithmetic mean,
(2) the constant temperature of 25 ℃ is needed for measuring the activity,
(3) the pyrogallol is prepared for use.
And (3) testing the high-temperature stability of enzyme activity:
incubating the product at high temperature (80 ℃) for 1h, then rapidly cooling to room temperature, comparing the enzyme activity before and after incubation, and expressing the enzyme activity preservation rate. The preservation rate of enzyme activity is equal to enzyme activity before high-temperature incubation/enzyme activity after high-temperature incubation multiplied by 100%.
The results of the preservation rate of the enzyme activity are shown in FIG. 2. As shown in fig. 2, the SOD, 5-HTP, magnesium malate, vitamins, etc. are compounded to show a stronger preservation rate of high temperature enzyme activity, wherein in example 2, the preservation rate of SOD enzyme activity at 80 ℃ is the highest, and is 99.64%. Compared with the SOD which is extracted from pig blood and has high temperature stability, the SOD high temperature enzyme activity preservation rate of the comparative example 8 is only 19.28 percent, which also shows that the invention can endure high temperature, maintain high activity for a long time and has wide application prospect.
Example 9
And (3) testing the anti-fatigue effect:
the antioxidant and anti-fatigue functions of the health care formula of the invention are explained by mouse experiments.
The test substances used in the experiment were: examples 1, 2, 3, 4, 5 and comparative examples 1, 2, 3, 4, 5, 6, 7, 8.
Animal sources and physical conditions used in the experiments: 270 normal healthy female mice with the weight of 18-20 g. The mice were randomly divided into 14 groups of 20 mice each, blank, experimental 1-5 groups, control 1-8 groups and blank.
The blank group was administered with physiological saline at a dose of 0.4mL/20g, and the test group and the control group were administered with the test substance at a dose of 5mg/20 g. The preparation is administered once daily for 10 days. On day 11, blood was collected from the orbit to determine SOD, and liver and brain cells were dissected to determine MDA content (malondialdehyde MDA detection kit).
Mouse swimming test: 10 mice were loaded with 5% weight and placed in a pool of 6cm × 6cm × 36cm for swimming at a water depth of 30cm and a water temperature of 25 ℃. + -. 0.5 ℃ at 30min after the last administration, and the time from the beginning of swimming to death of the mice was recorded as the swimming time of the mice.
Mouse pole climbing test: 10 mice in each group are taken, the test is started 30min after the last administration, a glass rod with the length of 1m and the diameter of 0.8cm is used, each mouse is exercised twice before the test, each time is 1min, the 3 rd formal test is carried out, and the rod climbing time of the mouse is recorded.
TABLE 3 antioxidant data of mice of different experimental groups
| Group of | Brain MDA | Liver MDA | SOD |
| Experimental group 1 | 18.55 | 5.24 | 271.36 |
| Experimental group 2 | 15.95 | 4.39 | 293.26 |
| Experimental group 3 | 16.82 | 4.49 | 291.24 |
| Experimental group 4 | 17.56 | 4.92 | 287.26 |
| Experimental group 5 | 17.69 | 4.99 | 289.98 |
| Control group 1 | 20.88 | 6.12 | 263.17 |
| Control group 2 | 17.35 | 4.67 | 290.87 |
| Control group 3 | 17.96 | 4.86 | 286.57 |
| Control group 4 | 18.48 | 5.13 | 273.49 |
| Control group 5 | 18.94 | 5.46 | 270.26 |
| Control group 6 | 19.36 | 5.79 | 269.18 |
| Control group 7 | 19.89 | 5.82 | 267.23 |
| Control group 8 | 20.16 | 5.97 | 265.94. |
| Blank group | 20.86 | 6.13 | 263.77 |
TABLE 4 anti-fatigue data of mice of different experimental groups
As can be seen from table 3, the experiment group 2 and the control group 1 clearly show that after the zhongkouxing SOD is added, the MDA content in the liver and brain cells of the mouse is obviously reduced, especially the MDA content in the liver and brain in the experiment group 2 is 30.90% and 39.41% respectively lower than the comparison ratio, and the activity of the SOD in the serum is 10.26% higher than that in the control group 1, which indicates that the zhongkoixing SOD has a removing effect on the MDA, and can also play a certain role in improving the activity of the serum SOD. Meanwhile, it can be seen from the control group 5 that the effect of Zhongkexin SOD is not achieved by replacing other antioxidant components. In addition, control 6 also shows that the direct mixing process has a poorer effect on the removal of mouse MDA than the batch slow mixing process. It can be seen from table 4 that, when melatonin is used to replace 5-HTP, the anti-fatigue effect of the mice is not as good as that of the experimental group 2, and the swimming time and pole-climbing time of the mice are significantly lower than that of the experimental group 2, which is probably because SOD and 5-HTP have a certain synergistic effect but do not have a synergistic effect with melatonin. In conclusion, the health formula of the experimental group 2 can obviously improve the activity of the mouse serum SOD and obviously reduce the MDA content in the mouse liver and brain cells, and the health formula has an antioxidant effect. And the test group can obviously prolong the swimming time and pole climbing time of the mice after taking the health-care formula, which shows that the invention has the functions of resisting fatigue and improving endurance.
Example 10
Sleep improvement test:
100 volunteers meeting inclusion criteria were publicly recruited and selected, and the selected subjects were healthy and had no other disorders affecting the study;
inclusion criteria were: the age is mainly 20-50 years old; screening people by using a Pittsburgh sleep quality index questionnaire, and bringing qualified volunteers into an experimental group; the resources can be tested and the record is finished;
a total of 140 satisfactory subjects were recruited for this trial and randomized into 13 groups of 10 subjects each. Set 5 of the working groups, 8 of the control groups, 1 of the blank groups. Tests were carried out using examples 1, 2, 3, 4, 5 and comparative examples 1, 2, 3, 4, 5, 6, 7, 8, respectively.
The taking method comprises the following steps: the product is taken with warm water one hour before sleep every day. The blank group did not swallow the drug, but was swallowed with warm water only.
The experiment time is 6 weeks, and the diet and living habits of volunteers are not changed during the experiment period. The volunteers took the samples continuously for 4 weeks, and were evaluated for efficacy at and after 2 weeks of discontinuation.
Evaluation indexes are as follows: the sleep quality adopts a Pittsburgh Sleep Quality Index (PSQI) self-evaluation table, and the total score is 0-21. The score is high or low and represents the sleep quality, and the lower the score, the better the sleep quality.
Calculating the formula: sleep quality improvement (4 weeks taken) is scored before taking-4 weeks taken;
sleep quality improvement (2 weeks off) is scored 4 weeks on-two weeks off;
the test group scores according to the PSQI evaluation are shown in table 5.
TABLE 5 comparison of sleep quality in the test groups
As can be seen from table 5, each experimental group had a certain improvement in sleep quality, in which example 2 showed better sleep improvement effect, while comparative example 2 did not improve sleep quality, probably because 5-HTP, an effective ingredient, was not added in comparative example 2. Meanwhile, even if the administration is stopped for 2 weeks, example 2 shows a certain sleep improvement effect, which also indicates that the health formula of the present invention has better persistence. In addition, it can be seen that the sleep-improving effect is also reduced in comparative example 3 and comparative example 4 compared to example 2 when magnesium malate or vitamins are not added. Furthermore, comparative example 7, in which 5-HTP was replaced with melatonin, did not have a strong sleep improvement effect as in example 2, and there are two possible reasons, that is, firstly, melatonin did not act synergistically with formulation components such as SOD, and further, melatonin was not effectively utilized by the human body because melatonin and 5-HTP are different in metabolic mechanisms of the human body, thereby exhibiting a poor sleep improvement effect. In conclusion, the health care formula disclosed by the invention adopts the compounding of SOD, 5-HTP, magnesium malate and the like, so that the synergistic effect is achieved, the scavenging effect of superoxide anion free radicals in a body can be effectively improved, the fatigue is resisted, and the sleep is improved.
Although the present invention has been described with reference to the preferred embodiments, it should be understood that various changes and modifications can be made therein by those skilled in the art without departing from the spirit and scope of the invention as defined in the appended claims.
Claims (10)
1. A health formula containing SOD and 5-HTP for resisting oxidation, resisting fatigue and improving sleep is characterized by comprising, by weight, 10-20 parts of superoxide dismutase, 10-25 parts of 5-hydroxytryptophan, 5-10 parts of magnesium malate, 0.01-0.1 part of vitamin and 10-20 parts of auxiliary materials.
2. The health formulation as claimed in claim 1, wherein the SOD unit enzyme activity is 10000-50000 IU/g.
3. The health formulation of claim 1, wherein the vitamins include one, more or all of vitamin a, vitamin D, vitamin E, vitamin C, vitamin B1, vitamin B2, vitamin B6, vitamin B12, niacin, folic acid, pantothenic acid, choline and niacinamide.
4. The health formulation according to claim 1, comprising, in parts by weight: SOD20 parts, 5-HTP25 parts, magnesium malate 10 parts, vitamins 0.07 part, and adjuvants 10-20 parts.
5. A health formulation according to any one of claims 1 to 4, wherein the vitamins are present in an amount of 0.07 parts by weight of each vitamin, including vitamin A0.7mg, vitamin E8 mg, vitamin C36 mg, vitamin D0.2 mg, vitamin B10.6 mg, vitamin B21.6 mg, vitamin B61.6mg, vitamin B125 mg, niacin 0.9mg, folic acid 0.7mg, pantothenic acid 4mg, choline 43mg, and nicotinamide 0.6 mg.
6. The health care formula according to claim 1, wherein the auxiliary materials comprise one, several or all of a filling agent, a flavoring agent, a glidant, a preservative, a binder and a disintegrating agent.
7. The health formula according to claim 1, wherein the filler is one, several or all of starch, dextrin and lactose; the correctant is one, more or all of Mel, simple syrup, pericarpium Citri Junoris syrup, fructus Pruni Pseudocerasi syrup and cortex Cinnamomi Japonici syrup; the glidant is one, more or all of talcum powder, micro-powder silica gel and silicon dioxide; the antiseptic is one, several or all of benzoic acid and its sodium salt, p-hydroxybenzoate and its sodium salt, sorbic acid and its potassium salt, and sodium acetate; the adhesive is one, several or all of starch slurry, microcrystalline cellulose, gelatin and polyvinyl alcohol; the disintegrant is one, several or all of dry starch, sodium carboxymethyl starch (CMS-Na), low-substituted hydroxypropyl cellulose (L-HPC), croscarmellose sodium (CCMC-Na), and crospovidone (PVPP).
8. The health formulation of claim 1, wherein the health formulation is in the form of a tablet, pill, powder, hard capsule, soft capsule, granule, oral liquid, beverage, candy, biscuit, or other food acceptable dosage form.
9. A method of preparing a health formulation according to any one of claims 1 to 8, comprising the steps of:
(1) weighing SOD, 5-HTP, magnesium malate, vitamins and auxiliary materials in a clean container according to the proportion;
(2) mixing and stirring 10-20 parts of SOD, 5-10 parts of magnesium malate and 20-60% of pure water by volume at the rotation speed of 600-800rpm and the temperature of 50-60 ℃, and cooling to 37 ℃ after stirring uniformly;
(3) slowly adding 5-HTP10-25 parts and 0.01-0.1 part of vitamin into the solution in the step (2) for multiple times, gradually adding 25-50% pure water by volume while stirring, and maintaining the conditions in the step (2) until the mixture is uniformly stirred;
(4) and finally, adding 10-20 parts of auxiliary materials and 10% -50% of pure water by volume, completely and uniformly stirring to obtain the required solution, and preparing into corresponding dosage forms for sterile subpackaging.
10. A health food comprising the health formulation of any one of claims 1 to 8.
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| CN103054044A (en) * | 2012-12-26 | 2013-04-24 | 北京康比特体育科技股份有限公司 | Sleep-improving health food composition |
| CN107412217A (en) * | 2007-03-22 | 2017-12-01 | 纽罗森特里亚股份有限公司 | Magnesium compositions and application thereof |
| CN111588040A (en) * | 2019-02-19 | 2020-08-28 | 李晨悦 | Health formula for resisting fatigue, whitening, moisturizing and dispelling effects of alcohol |
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| CN107412217A (en) * | 2007-03-22 | 2017-12-01 | 纽罗森特里亚股份有限公司 | Magnesium compositions and application thereof |
| CN103054044A (en) * | 2012-12-26 | 2013-04-24 | 北京康比特体育科技股份有限公司 | Sleep-improving health food composition |
| CN111588040A (en) * | 2019-02-19 | 2020-08-28 | 李晨悦 | Health formula for resisting fatigue, whitening, moisturizing and dispelling effects of alcohol |
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