CN114616326A - 使用新型大麻素合成酶由大麻萜酚酸生物合成大麻素 - Google Patents

使用新型大麻素合成酶由大麻萜酚酸生物合成大麻素 Download PDF

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CN114616326A
CN114616326A CN202080071171.7A CN202080071171A CN114616326A CN 114616326 A CN114616326 A CN 114616326A CN 202080071171 A CN202080071171 A CN 202080071171A CN 114616326 A CN114616326 A CN 114616326A
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梅贝尔·达琳·如·吴
姚文山
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Abstract

一种通过使大麻萜酚酸与大麻素合酶直系同源物接触来生产大麻素的方法。大麻素合酶直系同源物来自除大麻以外的生物体。本文还公开了酿酒酵母或毕赤酵母的重组细胞，在其基因组中包括编码上述大麻素合酶直系同源物的核酸。大麻素合酶直系同源物在重组细胞中以活性形式表达。

Description

使用新型大麻素合成酶由大麻萜酚酸生物合成大麻素
背景技术
大麻素生物合成途径一直是深入研究的主题。一些研究小组已经完成了大麻素生物合成途径中各种酶的克隆和表达。例如，已经阐明了Δ1-四氢大麻酚酸合酶(即大麻素合酶)的结构和功能。见Shoyama等人,J.Mol.Biol.2012,423(1):96-105。
大麻二酚酸和Δ9-四氢大麻酚酸这两种大麻素的生物合成是通过在酿酒酵母中异源表达来自大麻和其他生物体的大麻素生物合成途径酶来实现的。参见，例如，Luo等人,Nature 2019,567(7746):123-126和Zirpel等人,J.Biotechnol.2017,259:204-212。诸如此类的研究已经证实，在异源系统中构建大麻素生物合成途径是可能的。
亟需合成具有特异性及高产率的大麻素的新的酶和方法。
发明内容
本文公开了一种生产大麻素的方法。所述方法包括使大麻萜酚酸与大麻素合酶直系同源物接触的步骤。大麻素合酶直系同源物来自除大麻以外的生物体，例如橙子(Citrussinensis)、甜瓜(Cucumis melo)、辣椒(Capsicum annuum)、甘蓝型油菜(Brassicanapus)、烟草(Nicotiana attenuata)、红花烟草(Nicotiana tabacum)、天蓝遏蓝菜(Noccaea caerulescens)(遏蓝菜，Thlaspi caerulescens)、陆地棉(Gossypiumhirsutum,Gossypium mexicanum)、水稻亚种籼稻(Oryza sativa subsp.Indica)、水稻亚种粳稻(Oryza sativa subsp.japonica)、拟南芥亚种lyrata(Arabidopsislyratasubsp.lyrata)、类芽孢杆菌Aloe-11(Paenibacillus sp.Aloe-11)、链霉菌ipomoeae 91-03、芜菁(Brassica rapa subsp.pekinensis)、桃(Prunuspersica)、枯草芽孢杆菌168(Bacillussubtilis 168)、拟南芥(Arabidopsis thaliana)、罂粟(Papaversomniferum)和寄生疫霉P1569(PhytophthoraparasiticaP1569)。
本文还提供了酿酒酵母(Saccharomyces cerevisiae)和毕赤酵母(Pichiapastoris)的重组细胞，所述重组细胞各自在其基因组中包括编码大麻素合酶直系同源物的核酸，其中所述大麻素合酶直系同源物来自上一段中列出的任何生物体，并且所述大麻素合酶直系同源物在所述重组细胞中以活性形式表达。
在下文的说明书和实施例中对一个或多个实施方案进行了详细描述。根据详细描述、附图以及所附权利要求，其他特征、目的和优点将变得显而易见。
附图说明
下面参考附图描述本发明，其中：
图1示出了可由大麻萜酚酸(“CBGA”)作为底物形成的大麻素产物。六氢次大麻呋酚酸(“CBEA”)的分子式和分子量分别为C22H30O5和374.5。大麻酚素(cannabielsoin，脱羧CBEA)的分子式和分子量分别为C21H30O3和330.5。示出的其他化合物的分子式和分子量分别为C22H30O4和358.5。CBCA＝大麻二酚酸，CBTA＝大麻素酸(cannabicitranic acid)，CBDA＝大麻二酚酸，THICA＝四氢异大麻酚酸，THCA＝四氢大麻酚酸，CBLA＝大麻双环酸(cannabicyclolic acid)。
图2A显示了大麻素合酶直系同源物的18个反应组的代表性总离子色谱。每组反应耗时1.7min进行分析。
图2B显示了在质荷比(“m/z”)＝359.5下获得的大麻素合酶直系同源物的18个反应组的代表性萃取离子色谱图(“EIC”)，表明加入到18个反应组中的其中9个组(即组1-3、7-9、13-15)中存在CBGA。
图2C显示了18个反应组的代表性EIC，显示了m/z＝357.5的大麻素的产生。
图3A显示了一组18种大麻素合酶直系同源物的总离子色谱。每组反应耗时1.7min。
图3B显示了在m/z＝359.5处获得的大麻素合酶直系同源物的18个反应组中的代表性EIC，表明加入到18个反应组中的9个组(即组1-3、7-9、13-15)中存在CBGA。
图3C显示了18个反应组中的代表性EIC，显示了m/z＝357.5的大麻素的产生。
图4显示了产生m/z＝357.2071的大麻素的大麻素合酶直系同源物的代表性色谱图。每组反应耗时15.70min进行分析。第1行、第2行和第4行的色谱图显示，当CBGA与阳性大麻素合酶直系同源物一起孵育时，存在一个峰，该峰比在没有大麻素合酶直系同源物的情况下进行的对照实验(第3行)中发现的峰大65倍。
图5显示了产生m/z＝373.2020的大麻素的大麻素合酶直系同源物的代表性色谱图。每组反应耗时15.70min进行分析。第2-4行的色谱图显示，当CBGA与阳性大麻素合酶直系同源物一起孵育时，存在一个峰，该峰比在没有大麻素合酶直系同源物的情况下进行的对照实验(第1行)中发现的峰大120倍。
图6显示了产生m/z＝329.2122的大麻素的大麻素合酶直系同源物的代表性色谱图。每组反应耗时15.70min进行分析。第1行、第2行和第4行的色谱图显示，当CBGA与阳性大麻素合酶直系同源物一起孵育时，存在一个峰，该峰比在没有大麻素合酶直系同源物的情况下进行的对照实验(第3行)中发现的峰大300倍。
具体实施方式
本文公开了催化由CBGA生物合成大麻素的酶。这些以前不被称为大麻素合成酶的酶来自大麻以外的生物体。这些酶可以以活性形式在酿酒酵母和毕赤酵母中重组表达。
上文概括的生产大麻素的方法需要将CBGA与非大麻来源的大麻素合酶直系同源物接触。大麻素合酶直系同源物的来源可以是但不限于橙子、甘蓝型油菜、烟草、陆地棉、水稻亚种籼稻、水稻亚种粳稻、拟南芥亚种lyrata、类芽孢杆菌Aloe-11、链霉菌ipomoeae 91-03、芜菁、桃、枯草芽孢杆菌168、拟南芥、罂粟和寄生疫霉P1569。
大麻素合酶直系同源物可以是但不限于下表1和2中所示的那些。示例性大麻素合酶直系同源物可以具有SEQ ID NO:7、16、23、31、37、47、57、67、77、87、97、107、117和127的氨基酸序列，或具有与SEQ ID NO:7、16、23、31、37、47、57、67、77、87、97、107、117和127有70％或更高(例如，70％、75％、80％、85％、90％、95％和99％)同一性的序列。
上述方法可生产配方重量为358.5g/mol、374.5g/mol或330.5g/mol的大麻素。在特定方法中，分别生产配方重量为358.5g/mol、374.5g/mol和330.5g/mol的大麻素。
另一种方法生产六氢次大麻呋酚酸和大麻酚素。这种方法的一个实例是生产包含六氢次大麻呋酚酸和大麻酚素的大麻素，但产物不含配方重量为358.5g/mol的任何大麻素。在该示例性方法中，大麻素合酶直系同源物包括SEQ ID NO:67、77、97或127的氨基酸序列或与SEQ ID NO:67、77、97或127具有70％或更高同一性的序列。
在上述方法中，大麻素合酶直系同源物可以是重组酶。重组酶可以在例如酿酒酵母、解脂耶氏酵母(Yarrowia lipolytica)、马克斯克鲁维酵母(Kluyveromycesmarxianus)和毕赤酵母中产生。一种特定的方法以在酿酒酵母或毕赤酵母中产生的重组酶为特征。
上文还提到了酿酒酵母或毕赤酵母的重组细胞，在其基因组中含有编码大麻素合酶直系同源物的核酸。直系同源物来自大麻以外的生物体。大麻素合酶直系同源物的示例性来源已在上文列出，并如下文表1和表2中所示。
所述重组细胞可含有编码大麻素合酶直系同源物的核酸，所述直系同源物包括选自SEQ ID NO:7、16、23、31、37、47、57、67、77、87、97、107、117和127的氨基酸序列，或与SEQID NO:7、16、23、31、37、47、57、67、77、87、97、107、117和127具有70％或更高(例如，70％、75％、80％、85％、90％、95％和99％)同一性的序列。
无需进一步详述，相信本领域技术人员能够基于本文的公开内容最大程度地利用本公开内容。因此，以下具体实施例应被解释为仅仅是描述性的，而不是以任何方式限制本公开的其余部分。本文引用的所有出版物均通过引用整体并入本文作为参考。
实施例
实施例1：潜在大麻素合酶的鉴定和制备
根据其序列，THCA合成酶可归类为小檗碱桥FAD依赖性酶。通过搜索可用的序列数据库，鉴定出232个相关基因，在UniProt数据库中被注释为小檗碱桥FAD依赖酶。换言之，这232个基因是潜在的大麻素合酶直系同源物。
针对酿酒酵母蛋白表达对每个基因序列进行密码子优化，合成并克隆到pYES2-CT载体(Thermo Fisher)中。使用化学处理将载体分别转化到酿酒酵母BY4741细胞中，并在30℃下在SC-URA-葡萄糖选择平板上生长两天。从每个平板中挑选三个单菌落，复制到SC-URA-葡萄糖选择平板上，并在30℃孵育两天。复制的菌落在10mL SC-URA-葡萄糖培养基中于30℃培养16h。然后收获细胞并重新悬浮在5mL SC-URA-半乳糖培养基中，并在30℃下生长16h用于蛋白表达。16小时后，再次收获细胞并储存在-20℃下。未携带任何大麻素合酶直系同源物的细胞也按上述方法制备，用作阴性对照。
将细胞重新悬浮在400μL的100mM柠檬酸盐缓冲液(pH 5.5)中，加入1mM MgCl2和5个单位的溶细胞酶。在37℃下振荡培养细胞1h。向悬浮液中加入1g玻璃珠，并使用MPBiomedical FastPrep 24组织匀浆器将细胞裂解。离心30min，去除细胞碎片。转速为14800转/分，温度为4℃。
实施例2：酶活测定
如下测试了潜在的大麻素合酶直系同源物的酶活性。在55μL的反应体积中，将2.5μL的1mg/mL CBGA、2.5μL的20mM FAD和将如上制备的50μL的酵母细胞上清液合并，并在环境条件下孵育。还进行无CBGA的相应对照反应。24h后，用乙酸乙酯萃取3次。将提取材料的样本在真空下干燥，并重新溶于乙腈中，以使用负离子模式进行质量分析。对每份样本生成m/z＝357.5和m/z＝373.5的EIC，以确定大麻素的生物合成是否由直系同源物催化。潜在的反应产物如图1所示。
对于每种潜在的大麻素合酶直系同源物，如上所述挑选并培养了3个单酵母菌落。将从每个菌落制备的细胞上清液分成6份。3份与CBGA一起孵育，3份不与CBGA一起孵育作为阴性对照反应。总的来说，对于每一个潜在的直系同源物，在CBGA存在的情况下进行9个反应，不与CBGA一起孵育的进行9个反应。
如果提取的物质在EIC的色谱峰面积在m/z＝357.5和/或m/z＝373.5时丰度至少为800000，则与CBGA一起孵育的样本被视为大麻素合成阳性。如果使用上述标准时，与CBGA的9种总反应中有7种为阳性，则认为潜在大麻素合酶直系同源物为阳性。结果见下表1，以及图2A-图2C和图3A-图3C。
图2A-2C和3A-3C是Rapid-Fire/Triple Quad质量分析的代表性色谱图。图2C显示了m/z 357.5处存在一个峰的代表性色谱。图3C显示了比较m/z 373.5处峰的存在情况的代表性色谱图。
表1在酿酒酵母中表达的大麻素合酶直系同源物的活性。
Figure BDA0003588814280000051
Figure BDA0003588814280000061
在测试的72种大麻素合酶直系同源物中，20种显示产生分子式为C22H30O4的大麻素(MW＝358.5；m/z 357.5)和16种直系同源物显示产生了分子式为C22H30O4的大麻素(MW＝374.5；m/z 373.5)。5种直系同源物显示产生了两种大麻素。见上文表1。
实施例3：大麻素合酶在毕赤酵母中的同源表达
将编码以CBGA为底物表现出大麻素合酶活性的直系同源物的基因克隆到毕赤酵母表达系统中，以进行更大规模的蛋白质表达。THCA合成酶在毕赤酵母中的表达已被证实。参见，例如，Zirpel等人,Biotechnology Lett.2015,37(9):1869-1875和Lange等人,J.Biotechnol.2015,211:68-76。
将基因克隆到pPICZA质粒(Invitrogen)中，然后使用常规技术整合到毕赤酵母基因组中。从琼脂平板上接种所得到的毕赤酵母整合体，并在含有20mL缓冲复合甘油培养基的振荡培养三角瓶中于30℃以190rpm的转速振荡培养48h。离心收集细胞，并重新悬浮于200mL含0.5％w/v酪蛋白氨基酸和0.01％w/v核黄素的缓冲甲醇复合培养基中。每24h加入1％甲醇，共120h，诱导直系同源蛋白表达。
离心收集细胞，并重新悬浮在缓冲液A(100mM Tris，pH 8.0和150mM NaCl)中。使用M110P
Figure BDA0003588814280000062
(Microfluidics International Corp.)裂解细胞。通过离心去除细胞碎片，并将细胞裂解物加载到5mL StrepTrapTM HP色谱柱(Cytiva Life Sciences)上，流速为1mL/min。用25mL缓冲液A洗涤柱子，即5个柱体积(“CV”)，并用6个CV的缓冲液B(100mM Tris，pH 8.0，150mM NaCl和2.5mM去硫生物素)洗脱。通过280nm吸光度增加来鉴定的含蛋白质组分被汇集、浓缩并在-80℃冷冻。
在50μL的反应体积中，将从中纯化的大麻素合酶与150μM的CBGA和0.2mM的FAD在50mM柠檬酸盐(pH 8.0)中孵育。反应在30℃下孵育24-48h，然后加入160μL乙腈以停止反应并沉淀蛋白质。将混合物(120μL)注入Agilent InfinityLab Poroshell 120EC-C18色谱柱，使用Agilent 1290Infinity HPLC联用Agilent 6550iFunnel Q-TOF高分辨率质谱仪在负离子模式下进行质量分析。为每个样本生成了m/z＝357.2071、m/z＝373.2020和m/z＝329.2122的EIC，以确定潜在的大麻素的生物合成是否是由直系同源物催化。
结果见图4-图6和下表2。
表2在毕赤酵母中表达的大麻素合酶直系同源物的活性
Figure BDA0003588814280000071
在筛选的232种潜在CBS直系同源物中，7种直系同源物显示产生两种类型的大麻素，即(i)分子式为C22H30O4(FW＝358.5；m/z＝357.2071，见图4)的“大麻二酚酸基团”类型和(ii)六氢次大麻呋酚酸，分子式为C22H30O4(FW＝374.5；m/z＝373.2020，见图5)。还观察到六氢次大麻呋酚酸的脱羧产物，即大麻酚素，分子式为C21H30O2(FW＝330.5；m/z＝329.2122，见图6)。三种直系同源物只产生大麻二酚酸类的大麻素。四种直系同源物只产生六氢次大麻呋酚酸和大麻酚素。
其他实施例
本说明书中公开的所有特征可以以任何组合方式组合。本说明书中公开的每个特征可以被用于相同、等同或类似目的的替代特征所代替。因此，除非另有明确说明，否则所公开的每个特征仅是一系列等效或类似特征的示例。
通过以上描述，本领域技术人员可以容易地确定本发明的基本特征，并且在不脱离本发明的精神和范围的情况下，可以对本发明进行各种改变和修改，以使其适应各种用途和条件。因此，其他实施例也在所附权利要求的范围内。
序列表
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<120> 使用新型大麻素合成酶由大麻萜酚酸生物合成大麻素
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Val His Ala Leu Phe Thr Gly Leu Tyr Leu Gly Pro Ala Asn Asn Leu
245 250 255
ttg gcc ttg atg gaa gaa aag ttc cca gaa ttg ggt tta gaa aag gat 816
Leu Ala Leu Met Glu Glu Lys Phe Pro Glu Leu Gly Leu Glu Lys Asp
260 265 270
ggt tgt act gaa atg tcc tgg gtt gaa tct gtt ttg tgg ttt gct gat 864
Gly Cys Thr Glu Met Ser Trp Val Glu Ser Val Leu Trp Phe Ala Asp
275 280 285
ttc cac aag ggt gaa tct ttg gat gat gtt ttg acc aac aga gaa agg 912
Phe His Lys Gly Glu Ser Leu Asp Asp Val Leu Thr Asn Arg Glu Arg
290 295 300
acc tct ttg tct tac aaa ggt aag gat gat ttc gtg caa gag cca att 960
Thr Ser Leu Ser Tyr Lys Gly Lys Asp Asp Phe Val Gln Glu Pro Ile
305 310 315 320
cca gaa gct gct att caa gaa ctt tgg aga aga ttg gat gct cca gaa 1008
Pro Glu Ala Ala Ile Gln Glu Leu Trp Arg Arg Leu Asp Ala Pro Glu
325 330 335
gca aga ttg gcc aag att att ttg act cca ttt ggt ggt aag atg tcc 1056
Ala Arg Leu Ala Lys Ile Ile Leu Thr Pro Phe Gly Gly Lys Met Ser
340 345 350
gaa att gct gaa cac gaa act ttg ttc cca cat aga gaa ggt aac ttg 1104
Glu Ile Ala Glu His Glu Thr Leu Phe Pro His Arg Glu Gly Asn Leu
355 360 365
tac gaa att caa tac gtc gcc tat tgg aga gaa gaa gag gat aag aat 1152
Tyr Glu Ile Gln Tyr Val Ala Tyr Trp Arg Glu Glu Glu Asp Lys Asn
370 375 380
atg act ggc acc aac aaa tac ttg aag tgg gtt gat tca gtc tac gaa 1200
Met Thr Gly Thr Asn Lys Tyr Leu Lys Trp Val Asp Ser Val Tyr Glu
385 390 395 400
ttg atg act cca tac gtt tct aaa tct cca aga ggt gct tac gtt aac 1248
Leu Met Thr Pro Tyr Val Ser Lys Ser Pro Arg Gly Ala Tyr Val Asn
405 410 415
ttc gtt gat atg gat ttg ggt atg tac ctg ggt aag aaa aag acc aaa 1296
Phe Val Asp Met Asp Leu Gly Met Tyr Leu Gly Lys Lys Lys Thr Lys
420 425 430
tac gaa gag ggt aaa tcc tgg ggt gtt aag tac ttt aag aac aac ttc 1344
Tyr Glu Glu Gly Lys Ser Trp Gly Val Lys Tyr Phe Lys Asn Asn Phe
435 440 445
gaa aga ctg gtc aga gtc aag act tct gtt gat cca act gat ttc ttc 1392
Glu Arg Leu Val Arg Val Lys Thr Ser Val Asp Pro Thr Asp Phe Phe
450 455 460
tgc gac gaa caa tct att ccc ttg ttg aac aaa gtc act tgg tca cat 1440
Cys Asp Glu Gln Ser Ile Pro Leu Leu Asn Lys Val Thr Trp Ser His
465 470 475 480
cca caa ttt gaa aag 1455
Pro Gln Phe Glu Lys
485
<210> 10
<211> 485
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 10
Met Asn His Asn Thr Val Glu Ser Lys Val Ile His Thr Ser Lys Asp
1 5 10 15
Ser Ser Phe Phe Ser Ile Leu Asp Ser Ser Ile Gln Asn Pro Arg Phe
20 25 30
Ser Val Ser Glu Thr Pro Lys Pro Val Ser Ile Ile Thr Pro Val Lys
35 40 45
Ala Ser Asp Val Gln Thr Val Ile Arg Cys Ala Arg Leu His Gly Ile
50 55 60
His Val Arg Thr Arg Ser Ala Gly His Cys Tyr Glu Gly Leu Ser Tyr
65 70 75 80
Ile Ala Tyr Asn Lys Pro Phe Val Val Ile Asp Ile Arg Asn Leu Gln
85 90 95
Ser Ile Ser Leu Asp Val Asp Asn Arg Thr Gly Trp Val Gln Thr Gly
100 105 110
Ala Thr Ala Gly Glu Leu Tyr Tyr Glu Ile Gly Lys Thr Pro Lys Thr
115 120 125
Leu Ala Phe Pro Ala Gly Ile His Pro Thr Val Ala Ala Asp Asn Ile
130 135 140
Ile Asp Ala Leu Val Val Asp Ala Ser Gly Arg Ile Leu Asp Arg Gln
145 150 155 160
Ala Met Gly Glu Glu Tyr Tyr Phe Trp Ala Ile Cys Gly Gly Gly Gly
165 170 175
Ser Ser Phe Gly Ile Ile Leu Ser Trp Lys Ile Lys Leu Val Asp Val
180 185 190
Pro Ser Thr Ile Thr Val Phe Lys Val Lys Arg Thr Ser Lys Lys Glu
195 200 205
Ala Val Arg Ile Ile Asn Lys Trp Gln Tyr Val Ala Asp Lys Val Pro
210 215 220
Asp Asp Leu Phe Ile Arg Thr Thr Leu Glu Arg Ser Asn Lys Asn Ala
225 230 235 240
Val His Ala Leu Phe Thr Gly Leu Tyr Leu Gly Pro Ala Asn Asn Leu
245 250 255
Leu Ala Leu Met Glu Glu Lys Phe Pro Glu Leu Gly Leu Glu Lys Asp
260 265 270
Gly Cys Thr Glu Met Ser Trp Val Glu Ser Val Leu Trp Phe Ala Asp
275 280 285
Phe His Lys Gly Glu Ser Leu Asp Asp Val Leu Thr Asn Arg Glu Arg
290 295 300
Thr Ser Leu Ser Tyr Lys Gly Lys Asp Asp Phe Val Gln Glu Pro Ile
305 310 315 320
Pro Glu Ala Ala Ile Gln Glu Leu Trp Arg Arg Leu Asp Ala Pro Glu
325 330 335
Ala Arg Leu Ala Lys Ile Ile Leu Thr Pro Phe Gly Gly Lys Met Ser
340 345 350
Glu Ile Ala Glu His Glu Thr Leu Phe Pro His Arg Glu Gly Asn Leu
355 360 365
Tyr Glu Ile Gln Tyr Val Ala Tyr Trp Arg Glu Glu Glu Asp Lys Asn
370 375 380
Met Thr Gly Thr Asn Lys Tyr Leu Lys Trp Val Asp Ser Val Tyr Glu
385 390 395 400
Leu Met Thr Pro Tyr Val Ser Lys Ser Pro Arg Gly Ala Tyr Val Asn
405 410 415
Phe Val Asp Met Asp Leu Gly Met Tyr Leu Gly Lys Lys Lys Thr Lys
420 425 430
Tyr Glu Glu Gly Lys Ser Trp Gly Val Lys Tyr Phe Lys Asn Asn Phe
435 440 445
Glu Arg Leu Val Arg Val Lys Thr Ser Val Asp Pro Thr Asp Phe Phe
450 455 460
Cys Asp Glu Gln Ser Ile Pro Leu Leu Asn Lys Val Thr Trp Ser His
465 470 475 480
Pro Gln Phe Glu Lys
485
<210> 11
<211> 1512
<212> DNA
<213> 人工序列
<220>
<223> CBS1-D7MMG9-截断alpha因子strep标签
<220>
<221> CDS
<222> (1)..(1512)
<400> 11
atg aga ttt cct tcc atc ttc acg gct gtg cta ttt gca gca tcc tcc 48
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser
1 5 10 15
gca ctt gca atg aac cac aac acc gtt gaa tcc aag gtt att cat acc 96
Ala Leu Ala Met Asn His Asn Thr Val Glu Ser Lys Val Ile His Thr
20 25 30
tcc aag gac tct tca ttc ttc tcc att ttg gac tcc tct att cag aac 144
Ser Lys Asp Ser Ser Phe Phe Ser Ile Leu Asp Ser Ser Ile Gln Asn
35 40 45
cca aga ttc tct gtt tct gaa act cca aag cca gtt tcc att att act 192
Pro Arg Phe Ser Val Ser Glu Thr Pro Lys Pro Val Ser Ile Ile Thr
50 55 60
cca gtt aag gcc tct gat gtt caa acc gtt att aga tgt gct aga ttg 240
Pro Val Lys Ala Ser Asp Val Gln Thr Val Ile Arg Cys Ala Arg Leu
65 70 75 80
cat ggt atc cac gtt aga act aga tct gct ggt cat tgc tat gaa ggc 288
His Gly Ile His Val Arg Thr Arg Ser Ala Gly His Cys Tyr Glu Gly
85 90 95
ttg tct tat att gct tac aac aag cca ttc gtc gtc atc gac att aga 336
Leu Ser Tyr Ile Ala Tyr Asn Lys Pro Phe Val Val Ile Asp Ile Arg
100 105 110
aac ttg caa tcc atc tcc ttg gat gtt gat aat aga act ggt tgg gtt 384
Asn Leu Gln Ser Ile Ser Leu Asp Val Asp Asn Arg Thr Gly Trp Val
115 120 125
caa act ggt gct act gct ggt gaa cta tat tac gaa att ggt aag act 432
Gln Thr Gly Ala Thr Ala Gly Glu Leu Tyr Tyr Glu Ile Gly Lys Thr
130 135 140
cca aag acc ttg gct ttt cca gct ggt att cat cca act gtt gct gcc 480
Pro Lys Thr Leu Ala Phe Pro Ala Gly Ile His Pro Thr Val Ala Ala
145 150 155 160
gat aac att att gat gct ttg gtt gtt gat gcc tcc ggt aga att ttg 528
Asp Asn Ile Ile Asp Ala Leu Val Val Asp Ala Ser Gly Arg Ile Leu
165 170 175
gat aga caa gct atg ggt gaa gag tac tac ttt tgg gct att tgc ggt 576
Asp Arg Gln Ala Met Gly Glu Glu Tyr Tyr Phe Trp Ala Ile Cys Gly
180 185 190
ggt ggt ggt tct tct ttt ggt att atc ttg tcc tgg aag atc aag ttg 624
Gly Gly Gly Ser Ser Phe Gly Ile Ile Leu Ser Trp Lys Ile Lys Leu
195 200 205
gtt gat gtt cca tct acc atc act gtt ttc aag gtt aag agg acc tct 672
Val Asp Val Pro Ser Thr Ile Thr Val Phe Lys Val Lys Arg Thr Ser
210 215 220
aag aaa gaa gcc gtt aga atc att aac aag tgg caa tac gtt gcc gat 720
Lys Lys Glu Ala Val Arg Ile Ile Asn Lys Trp Gln Tyr Val Ala Asp
225 230 235 240
aag gtt cca gat gat ttg ttc att aga acc acc ttg gaa agg tct aac 768
Lys Val Pro Asp Asp Leu Phe Ile Arg Thr Thr Leu Glu Arg Ser Asn
245 250 255
aag aat gct gtt cat gct ttg ttt acc ggc ttg tat ttg ggt cca gct 816
Lys Asn Ala Val His Ala Leu Phe Thr Gly Leu Tyr Leu Gly Pro Ala
260 265 270
aac aat ttg ttg gcc ttg atg gaa gaa aag ttc cca gaa ttg ggt tta 864
Asn Asn Leu Leu Ala Leu Met Glu Glu Lys Phe Pro Glu Leu Gly Leu
275 280 285
gaa aag gat ggt tgt act gaa atg tcc tgg gtt gaa tct gtt ttg tgg 912
Glu Lys Asp Gly Cys Thr Glu Met Ser Trp Val Glu Ser Val Leu Trp
290 295 300
ttt gct gat ttc cac aag ggt gaa tct ttg gat gat gtt ttg acc aac 960
Phe Ala Asp Phe His Lys Gly Glu Ser Leu Asp Asp Val Leu Thr Asn
305 310 315 320
aga gaa agg acc tct ttg tct tac aaa ggt aag gat gat ttc gtg caa 1008
Arg Glu Arg Thr Ser Leu Ser Tyr Lys Gly Lys Asp Asp Phe Val Gln
325 330 335
gag cca att cca gaa gct gct att caa gaa ctt tgg aga aga ttg gat 1056
Glu Pro Ile Pro Glu Ala Ala Ile Gln Glu Leu Trp Arg Arg Leu Asp
340 345 350
gct cca gaa gca aga ttg gcc aag att att ttg act cca ttt ggt ggt 1104
Ala Pro Glu Ala Arg Leu Ala Lys Ile Ile Leu Thr Pro Phe Gly Gly
355 360 365
aag atg tcc gaa att gct gaa cac gaa act ttg ttc cca cat aga gaa 1152
Lys Met Ser Glu Ile Ala Glu His Glu Thr Leu Phe Pro His Arg Glu
370 375 380
ggt aac ttg tac gaa att caa tac gtc gcc tat tgg aga gaa gaa gag 1200
Gly Asn Leu Tyr Glu Ile Gln Tyr Val Ala Tyr Trp Arg Glu Glu Glu
385 390 395 400
gat aag aat atg act ggc acc aac aaa tac ttg aag tgg gtt gat tca 1248
Asp Lys Asn Met Thr Gly Thr Asn Lys Tyr Leu Lys Trp Val Asp Ser
405 410 415
gtc tac gaa ttg atg act cca tac gtt tct aaa tct cca aga ggt gct 1296
Val Tyr Glu Leu Met Thr Pro Tyr Val Ser Lys Ser Pro Arg Gly Ala
420 425 430
tac gtt aac ttc gtt gat atg gat ttg ggt atg tac ctg ggt aag aaa 1344
Tyr Val Asn Phe Val Asp Met Asp Leu Gly Met Tyr Leu Gly Lys Lys
435 440 445
aag acc aaa tac gaa gag ggt aaa tcc tgg ggt gtt aag tac ttt aag 1392
Lys Thr Lys Tyr Glu Glu Gly Lys Ser Trp Gly Val Lys Tyr Phe Lys
450 455 460
aac aac ttc gaa aga ctg gtc aga gtc aag act tct gtt gat cca act 1440
Asn Asn Phe Glu Arg Leu Val Arg Val Lys Thr Ser Val Asp Pro Thr
465 470 475 480
gat ttc ttc tgc gac gaa caa tct att ccc ttg ttg aac aaa gtc act 1488
Asp Phe Phe Cys Asp Glu Gln Ser Ile Pro Leu Leu Asn Lys Val Thr
485 490 495
tgg tca cat cca caa ttt gaa aag 1512
Trp Ser His Pro Gln Phe Glu Lys
500
<210> 12
<211> 504
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 12
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser
1 5 10 15
Ala Leu Ala Met Asn His Asn Thr Val Glu Ser Lys Val Ile His Thr
20 25 30
Ser Lys Asp Ser Ser Phe Phe Ser Ile Leu Asp Ser Ser Ile Gln Asn
35 40 45
Pro Arg Phe Ser Val Ser Glu Thr Pro Lys Pro Val Ser Ile Ile Thr
50 55 60
Pro Val Lys Ala Ser Asp Val Gln Thr Val Ile Arg Cys Ala Arg Leu
65 70 75 80
His Gly Ile His Val Arg Thr Arg Ser Ala Gly His Cys Tyr Glu Gly
85 90 95
Leu Ser Tyr Ile Ala Tyr Asn Lys Pro Phe Val Val Ile Asp Ile Arg
100 105 110
Asn Leu Gln Ser Ile Ser Leu Asp Val Asp Asn Arg Thr Gly Trp Val
115 120 125
Gln Thr Gly Ala Thr Ala Gly Glu Leu Tyr Tyr Glu Ile Gly Lys Thr
130 135 140
Pro Lys Thr Leu Ala Phe Pro Ala Gly Ile His Pro Thr Val Ala Ala
145 150 155 160
Asp Asn Ile Ile Asp Ala Leu Val Val Asp Ala Ser Gly Arg Ile Leu
165 170 175
Asp Arg Gln Ala Met Gly Glu Glu Tyr Tyr Phe Trp Ala Ile Cys Gly
180 185 190
Gly Gly Gly Ser Ser Phe Gly Ile Ile Leu Ser Trp Lys Ile Lys Leu
195 200 205
Val Asp Val Pro Ser Thr Ile Thr Val Phe Lys Val Lys Arg Thr Ser
210 215 220
Lys Lys Glu Ala Val Arg Ile Ile Asn Lys Trp Gln Tyr Val Ala Asp
225 230 235 240
Lys Val Pro Asp Asp Leu Phe Ile Arg Thr Thr Leu Glu Arg Ser Asn
245 250 255
Lys Asn Ala Val His Ala Leu Phe Thr Gly Leu Tyr Leu Gly Pro Ala
260 265 270
Asn Asn Leu Leu Ala Leu Met Glu Glu Lys Phe Pro Glu Leu Gly Leu
275 280 285
Glu Lys Asp Gly Cys Thr Glu Met Ser Trp Val Glu Ser Val Leu Trp
290 295 300
Phe Ala Asp Phe His Lys Gly Glu Ser Leu Asp Asp Val Leu Thr Asn
305 310 315 320
Arg Glu Arg Thr Ser Leu Ser Tyr Lys Gly Lys Asp Asp Phe Val Gln
325 330 335
Glu Pro Ile Pro Glu Ala Ala Ile Gln Glu Leu Trp Arg Arg Leu Asp
340 345 350
Ala Pro Glu Ala Arg Leu Ala Lys Ile Ile Leu Thr Pro Phe Gly Gly
355 360 365
Lys Met Ser Glu Ile Ala Glu His Glu Thr Leu Phe Pro His Arg Glu
370 375 380
Gly Asn Leu Tyr Glu Ile Gln Tyr Val Ala Tyr Trp Arg Glu Glu Glu
385 390 395 400
Asp Lys Asn Met Thr Gly Thr Asn Lys Tyr Leu Lys Trp Val Asp Ser
405 410 415
Val Tyr Glu Leu Met Thr Pro Tyr Val Ser Lys Ser Pro Arg Gly Ala
420 425 430
Tyr Val Asn Phe Val Asp Met Asp Leu Gly Met Tyr Leu Gly Lys Lys
435 440 445
Lys Thr Lys Tyr Glu Glu Gly Lys Ser Trp Gly Val Lys Tyr Phe Lys
450 455 460
Asn Asn Phe Glu Arg Leu Val Arg Val Lys Thr Ser Val Asp Pro Thr
465 470 475 480
Asp Phe Phe Cys Asp Glu Gln Ser Ile Pro Leu Leu Asn Lys Val Thr
485 490 495
Trp Ser His Pro Gln Phe Glu Lys
500
<210> 13
<211> 1722
<212> DNA
<213> 人工序列
<220>
<223> CBS1-D7MMG9-全长alpha因子-strep标签
<220>
<221> CDS
<222> (1)..(1722)
<400> 13
atg aga ttt cct tca att ttt act gct gtt tta ttc gca gca tcc tcc 48
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser
1 5 10 15
gca tta gct gct cca gtc aac act aca aca gaa gat gaa acg gca caa 96
Ala Leu Ala Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln
20 25 30
att ccg gct gaa gct gtc atc ggt tac tca gat tta gaa ggg gat ttc 144
Ile Pro Ala Glu Ala Val Ile Gly Tyr Ser Asp Leu Glu Gly Asp Phe
35 40 45
gat gtt gct gtt ttg cca ttt tcc aac agc aca aat aac ggg tta ttg 192
Asp Val Ala Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu
50 55 60
ttt ata aat act act att gcc agc att gct gct aaa gaa gaa ggg gta 240
Phe Ile Asn Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val
65 70 75 80
tct ctc gag aaa aga gag gct gaa gct atg aac cac aac acc gtt gaa 288
Ser Leu Glu Lys Arg Glu Ala Glu Ala Met Asn His Asn Thr Val Glu
85 90 95
tcc aag gtt att cat acc tcc aag gac tct tca ttc ttc tcc att ttg 336
Ser Lys Val Ile His Thr Ser Lys Asp Ser Ser Phe Phe Ser Ile Leu
100 105 110
gac tcc tct att cag aac cca aga ttc tct gtt tct gaa act cca aag 384
Asp Ser Ser Ile Gln Asn Pro Arg Phe Ser Val Ser Glu Thr Pro Lys
115 120 125
cca gtt tcc att att act cca gtt aag gcc tct gat gtt caa acc gtt 432
Pro Val Ser Ile Ile Thr Pro Val Lys Ala Ser Asp Val Gln Thr Val
130 135 140
att aga tgt gct aga ttg cat ggt atc cac gtt aga act aga tct gct 480
Ile Arg Cys Ala Arg Leu His Gly Ile His Val Arg Thr Arg Ser Ala
145 150 155 160
ggt cat tgc tat gaa ggc ttg tct tat att gct tac aac aag cca ttc 528
Gly His Cys Tyr Glu Gly Leu Ser Tyr Ile Ala Tyr Asn Lys Pro Phe
165 170 175
gtc gtc atc gac att aga aac ttg caa tcc atc tcc ttg gat gtt gat 576
Val Val Ile Asp Ile Arg Asn Leu Gln Ser Ile Ser Leu Asp Val Asp
180 185 190
aat aga act ggt tgg gtt caa act ggt gct act gct ggt gaa cta tat 624
Asn Arg Thr Gly Trp Val Gln Thr Gly Ala Thr Ala Gly Glu Leu Tyr
195 200 205
tac gaa att ggt aag act cca aag acc ttg gct ttt cca gct ggt att 672
Tyr Glu Ile Gly Lys Thr Pro Lys Thr Leu Ala Phe Pro Ala Gly Ile
210 215 220
cat cca act gtt gct gcc gat aac att att gat gct ttg gtt gtt gat 720
His Pro Thr Val Ala Ala Asp Asn Ile Ile Asp Ala Leu Val Val Asp
225 230 235 240
gcc tcc ggt aga att ttg gat aga caa gct atg ggt gaa gag tac tac 768
Ala Ser Gly Arg Ile Leu Asp Arg Gln Ala Met Gly Glu Glu Tyr Tyr
245 250 255
ttt tgg gct att tgc ggt ggt ggt ggt tct tct ttt ggt att atc ttg 816
Phe Trp Ala Ile Cys Gly Gly Gly Gly Ser Ser Phe Gly Ile Ile Leu
260 265 270
tcc tgg aag atc aag ttg gtt gat gtt cca tct acc atc act gtt ttc 864
Ser Trp Lys Ile Lys Leu Val Asp Val Pro Ser Thr Ile Thr Val Phe
275 280 285
aag gtt aag agg acc tct aag aaa gaa gcc gtt aga atc att aac aag 912
Lys Val Lys Arg Thr Ser Lys Lys Glu Ala Val Arg Ile Ile Asn Lys
290 295 300
tgg caa tac gtt gcc gat aag gtt cca gat gat ttg ttc att aga acc 960
Trp Gln Tyr Val Ala Asp Lys Val Pro Asp Asp Leu Phe Ile Arg Thr
305 310 315 320
acc ttg gaa agg tct aac aag aat gct gtt cat gct ttg ttt acc ggc 1008
Thr Leu Glu Arg Ser Asn Lys Asn Ala Val His Ala Leu Phe Thr Gly
325 330 335
ttg tat ttg ggt cca gct aac aat ttg ttg gcc ttg atg gaa gaa aag 1056
Leu Tyr Leu Gly Pro Ala Asn Asn Leu Leu Ala Leu Met Glu Glu Lys
340 345 350
ttc cca gaa ttg ggt tta gaa aag gat ggt tgt act gaa atg tcc tgg 1104
Phe Pro Glu Leu Gly Leu Glu Lys Asp Gly Cys Thr Glu Met Ser Trp
355 360 365
gtt gaa tct gtt ttg tgg ttt gct gat ttc cac aag ggt gaa tct ttg 1152
Val Glu Ser Val Leu Trp Phe Ala Asp Phe His Lys Gly Glu Ser Leu
370 375 380
gat gat gtt ttg acc aac aga gaa agg acc tct ttg tct tac aaa ggt 1200
Asp Asp Val Leu Thr Asn Arg Glu Arg Thr Ser Leu Ser Tyr Lys Gly
385 390 395 400
aag gat gat ttc gtg caa gag cca att cca gaa gct gct att caa gaa 1248
Lys Asp Asp Phe Val Gln Glu Pro Ile Pro Glu Ala Ala Ile Gln Glu
405 410 415
ctt tgg aga aga ttg gat gct cca gaa gca aga ttg gcc aag att att 1296
Leu Trp Arg Arg Leu Asp Ala Pro Glu Ala Arg Leu Ala Lys Ile Ile
420 425 430
ttg act cca ttt ggt ggt aag atg tcc gaa att gct gaa cac gaa act 1344
Leu Thr Pro Phe Gly Gly Lys Met Ser Glu Ile Ala Glu His Glu Thr
435 440 445
ttg ttc cca cat aga gaa ggt aac ttg tac gaa att caa tac gtc gcc 1392
Leu Phe Pro His Arg Glu Gly Asn Leu Tyr Glu Ile Gln Tyr Val Ala
450 455 460
tat tgg aga gaa gaa gag gat aag aat atg act ggc acc aac aaa tac 1440
Tyr Trp Arg Glu Glu Glu Asp Lys Asn Met Thr Gly Thr Asn Lys Tyr
465 470 475 480
ttg aag tgg gtt gat tca gtc tac gaa ttg atg act cca tac gtt tct 1488
Leu Lys Trp Val Asp Ser Val Tyr Glu Leu Met Thr Pro Tyr Val Ser
485 490 495
aaa tct cca aga ggt gct tac gtt aac ttc gtt gat atg gat ttg ggt 1536
Lys Ser Pro Arg Gly Ala Tyr Val Asn Phe Val Asp Met Asp Leu Gly
500 505 510
atg tac ctg ggt aag aaa aag acc aaa tac gaa gag ggt aaa tcc tgg 1584
Met Tyr Leu Gly Lys Lys Lys Thr Lys Tyr Glu Glu Gly Lys Ser Trp
515 520 525
ggt gtt aag tac ttt aag aac aac ttc gaa aga ctg gtc aga gtc aag 1632
Gly Val Lys Tyr Phe Lys Asn Asn Phe Glu Arg Leu Val Arg Val Lys
530 535 540
act tct gtt gat cca act gat ttc ttc tgc gac gaa caa tct att ccc 1680
Thr Ser Val Asp Pro Thr Asp Phe Phe Cys Asp Glu Gln Ser Ile Pro
545 550 555 560
ttg ttg aac aaa gtc act tgg tca cat cca caa ttt gaa aag 1722
Leu Leu Asn Lys Val Thr Trp Ser His Pro Gln Phe Glu Lys
565 570
<210> 14
<211> 574
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 14
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser
1 5 10 15
Ala Leu Ala Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln
20 25 30
Ile Pro Ala Glu Ala Val Ile Gly Tyr Ser Asp Leu Glu Gly Asp Phe
35 40 45
Asp Val Ala Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu
50 55 60
Phe Ile Asn Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val
65 70 75 80
Ser Leu Glu Lys Arg Glu Ala Glu Ala Met Asn His Asn Thr Val Glu
85 90 95
Ser Lys Val Ile His Thr Ser Lys Asp Ser Ser Phe Phe Ser Ile Leu
100 105 110
Asp Ser Ser Ile Gln Asn Pro Arg Phe Ser Val Ser Glu Thr Pro Lys
115 120 125
Pro Val Ser Ile Ile Thr Pro Val Lys Ala Ser Asp Val Gln Thr Val
130 135 140
Ile Arg Cys Ala Arg Leu His Gly Ile His Val Arg Thr Arg Ser Ala
145 150 155 160
Gly His Cys Tyr Glu Gly Leu Ser Tyr Ile Ala Tyr Asn Lys Pro Phe
165 170 175
Val Val Ile Asp Ile Arg Asn Leu Gln Ser Ile Ser Leu Asp Val Asp
180 185 190
Asn Arg Thr Gly Trp Val Gln Thr Gly Ala Thr Ala Gly Glu Leu Tyr
195 200 205
Tyr Glu Ile Gly Lys Thr Pro Lys Thr Leu Ala Phe Pro Ala Gly Ile
210 215 220
His Pro Thr Val Ala Ala Asp Asn Ile Ile Asp Ala Leu Val Val Asp
225 230 235 240
Ala Ser Gly Arg Ile Leu Asp Arg Gln Ala Met Gly Glu Glu Tyr Tyr
245 250 255
Phe Trp Ala Ile Cys Gly Gly Gly Gly Ser Ser Phe Gly Ile Ile Leu
260 265 270
Ser Trp Lys Ile Lys Leu Val Asp Val Pro Ser Thr Ile Thr Val Phe
275 280 285
Lys Val Lys Arg Thr Ser Lys Lys Glu Ala Val Arg Ile Ile Asn Lys
290 295 300
Trp Gln Tyr Val Ala Asp Lys Val Pro Asp Asp Leu Phe Ile Arg Thr
305 310 315 320
Thr Leu Glu Arg Ser Asn Lys Asn Ala Val His Ala Leu Phe Thr Gly
325 330 335
Leu Tyr Leu Gly Pro Ala Asn Asn Leu Leu Ala Leu Met Glu Glu Lys
340 345 350
Phe Pro Glu Leu Gly Leu Glu Lys Asp Gly Cys Thr Glu Met Ser Trp
355 360 365
Val Glu Ser Val Leu Trp Phe Ala Asp Phe His Lys Gly Glu Ser Leu
370 375 380
Asp Asp Val Leu Thr Asn Arg Glu Arg Thr Ser Leu Ser Tyr Lys Gly
385 390 395 400
Lys Asp Asp Phe Val Gln Glu Pro Ile Pro Glu Ala Ala Ile Gln Glu
405 410 415
Leu Trp Arg Arg Leu Asp Ala Pro Glu Ala Arg Leu Ala Lys Ile Ile
420 425 430
Leu Thr Pro Phe Gly Gly Lys Met Ser Glu Ile Ala Glu His Glu Thr
435 440 445
Leu Phe Pro His Arg Glu Gly Asn Leu Tyr Glu Ile Gln Tyr Val Ala
450 455 460
Tyr Trp Arg Glu Glu Glu Asp Lys Asn Met Thr Gly Thr Asn Lys Tyr
465 470 475 480
Leu Lys Trp Val Asp Ser Val Tyr Glu Leu Met Thr Pro Tyr Val Ser
485 490 495
Lys Ser Pro Arg Gly Ala Tyr Val Asn Phe Val Asp Met Asp Leu Gly
500 505 510
Met Tyr Leu Gly Lys Lys Lys Thr Lys Tyr Glu Glu Gly Lys Ser Trp
515 520 525
Gly Val Lys Tyr Phe Lys Asn Asn Phe Glu Arg Leu Val Arg Val Lys
530 535 540
Thr Ser Val Asp Pro Thr Asp Phe Phe Cys Asp Glu Gln Ser Ile Pro
545 550 555 560
Leu Leu Asn Lys Val Thr Trp Ser His Pro Gln Phe Glu Lys
565 570
<210> 15
<211> 1011
<212> DNA
<213> 人工序列
<220>
<223> CBS5-M4DIE5
<220>
<221> CDS
<222> (1)..(1011)
<400> 15
atg agg aag tac ggt ttg acc gtt gat aat gtt gtt gat gcc ttg atc 48
Met Arg Lys Tyr Gly Leu Thr Val Asp Asn Val Val Asp Ala Leu Ile
1 5 10 15
gtt gat gtt aac ggc aag ttg ttg aac aga tct act atg ggt gaa gat 96
Val Asp Val Asn Gly Lys Leu Leu Asn Arg Ser Thr Met Gly Glu Asp
20 25 30
ttg ttc tgg gct att aga ggt ggt ggt ggc ggt tct ttt ggt gtt att 144
Leu Phe Trp Ala Ile Arg Gly Gly Gly Gly Gly Ser Phe Gly Val Ile
35 40 45
ttg tct tgg aag atc aac ttg gtt gac gtc cca gaa att ttg act gtt 192
Leu Ser Trp Lys Ile Asn Leu Val Asp Val Pro Glu Ile Leu Thr Val
50 55 60
ttc aga gtt aac aag acc ttg gaa caa ggt ggt att gac gtc ttg tac 240
Phe Arg Val Asn Lys Thr Leu Glu Gln Gly Gly Ile Asp Val Leu Tyr
65 70 75 80
aaa tgg caa ttg gtg tcc tct aag ttg ttg gat ggt ttg ttt ttg aga 288
Lys Trp Gln Leu Val Ser Ser Lys Leu Leu Asp Gly Leu Phe Leu Arg
85 90 95
gcc ttg tct caa gtt gtc aac aga act atc acc gtt gaa ttt tac gct 336
Ala Leu Ser Gln Val Val Asn Arg Thr Ile Thr Val Glu Phe Tyr Ala
100 105 110
cag ttt ttg ggt aga gcc gat gaa ttg gtt gct atc gtt aat caa tct 384
Gln Phe Leu Gly Arg Ala Asp Glu Leu Val Ala Ile Val Asn Gln Ser
115 120 125
ttg cca gag ttg ggt ttg aag aga caa gat tgc ttg gaa atg tct tgg 432
Leu Pro Glu Leu Gly Leu Lys Arg Gln Asp Cys Leu Glu Met Ser Trp
130 135 140
ttg aac act acc ttg ttt tgg gaa gat ttg cca gtt ggt act cca act 480
Leu Asn Thr Thr Leu Phe Trp Glu Asp Leu Pro Val Gly Thr Pro Thr
145 150 155 160
tct gtt ttg ttg gat aga cca tct aag cca gag aag ttc ttc aag tct 528
Ser Val Leu Leu Asp Arg Pro Ser Lys Pro Glu Lys Phe Phe Lys Ser
165 170 175
aaa tcc gat tac gtc aag aag ccc att cca aaa gag ggt att aag aaa 576
Lys Ser Asp Tyr Val Lys Lys Pro Ile Pro Lys Glu Gly Ile Lys Lys
180 185 190
ttg tgg gaa gcc ttg ttg gag ttt aac aac aac aat atc gtc tac atg 624
Leu Trp Glu Ala Leu Leu Glu Phe Asn Asn Asn Asn Ile Val Tyr Met
195 200 205
cag tgg aat cca tac ggt ggt gtt atg gat act gtt cca gct gct gct 672
Gln Trp Asn Pro Tyr Gly Gly Val Met Asp Thr Val Pro Ala Ala Ala
210 215 220
act cca ttt cca cat aga aaa ggt aac ttg ttc gaa gtc cag tac tac 720
Thr Pro Phe Pro His Arg Lys Gly Asn Leu Phe Glu Val Gln Tyr Tyr
225 230 235 240
act tct tgg ttg gat gct aat gct aca atg ggt tct ttg gac atg atg 768
Thr Ser Trp Leu Asp Ala Asn Ala Thr Met Gly Ser Leu Asp Met Met
245 250 255
aag aag ttg tac gaa gtt gct gaa ccc tac gtt tct tct aat cca aga 816
Lys Lys Leu Tyr Glu Val Ala Glu Pro Tyr Val Ser Ser Asn Pro Arg
260 265 270
gaa gcc ttt ttg aac tac aga gac atc gac att ggt agc aat cca tct 864
Glu Ala Phe Leu Asn Tyr Arg Asp Ile Asp Ile Gly Ser Asn Pro Ser
275 280 285
aac gaa act aat gtt gaa gaa gcc gaa atc tac ggc tct aag tac ttt 912
Asn Glu Thr Asn Val Glu Glu Ala Glu Ile Tyr Gly Ser Lys Tyr Phe
290 295 300
gtt ggt aac ttg aag aga ttg atg gaa gtc aag gct aag tac gat cca 960
Val Gly Asn Leu Lys Arg Leu Met Glu Val Lys Ala Lys Tyr Asp Pro
305 310 315 320
gaa aac ttt ttc aag aac gag caa tct att cca cca gcc aga gtt aag 1008
Glu Asn Phe Phe Lys Asn Glu Gln Ser Ile Pro Pro Ala Arg Val Lys
325 330 335
caa 1011
Gln
<210> 16
<211> 337
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 16
Met Arg Lys Tyr Gly Leu Thr Val Asp Asn Val Val Asp Ala Leu Ile
1 5 10 15
Val Asp Val Asn Gly Lys Leu Leu Asn Arg Ser Thr Met Gly Glu Asp
20 25 30
Leu Phe Trp Ala Ile Arg Gly Gly Gly Gly Gly Ser Phe Gly Val Ile
35 40 45
Leu Ser Trp Lys Ile Asn Leu Val Asp Val Pro Glu Ile Leu Thr Val
50 55 60
Phe Arg Val Asn Lys Thr Leu Glu Gln Gly Gly Ile Asp Val Leu Tyr
65 70 75 80
Lys Trp Gln Leu Val Ser Ser Lys Leu Leu Asp Gly Leu Phe Leu Arg
85 90 95
Ala Leu Ser Gln Val Val Asn Arg Thr Ile Thr Val Glu Phe Tyr Ala
100 105 110
Gln Phe Leu Gly Arg Ala Asp Glu Leu Val Ala Ile Val Asn Gln Ser
115 120 125
Leu Pro Glu Leu Gly Leu Lys Arg Gln Asp Cys Leu Glu Met Ser Trp
130 135 140
Leu Asn Thr Thr Leu Phe Trp Glu Asp Leu Pro Val Gly Thr Pro Thr
145 150 155 160
Ser Val Leu Leu Asp Arg Pro Ser Lys Pro Glu Lys Phe Phe Lys Ser
165 170 175
Lys Ser Asp Tyr Val Lys Lys Pro Ile Pro Lys Glu Gly Ile Lys Lys
180 185 190
Leu Trp Glu Ala Leu Leu Glu Phe Asn Asn Asn Asn Ile Val Tyr Met
195 200 205
Gln Trp Asn Pro Tyr Gly Gly Val Met Asp Thr Val Pro Ala Ala Ala
210 215 220
Thr Pro Phe Pro His Arg Lys Gly Asn Leu Phe Glu Val Gln Tyr Tyr
225 230 235 240
Thr Ser Trp Leu Asp Ala Asn Ala Thr Met Gly Ser Leu Asp Met Met
245 250 255
Lys Lys Leu Tyr Glu Val Ala Glu Pro Tyr Val Ser Ser Asn Pro Arg
260 265 270
Glu Ala Phe Leu Asn Tyr Arg Asp Ile Asp Ile Gly Ser Asn Pro Ser
275 280 285
Asn Glu Thr Asn Val Glu Glu Ala Glu Ile Tyr Gly Ser Lys Tyr Phe
290 295 300
Val Gly Asn Leu Lys Arg Leu Met Glu Val Lys Ala Lys Tyr Asp Pro
305 310 315 320
Glu Asn Phe Phe Lys Asn Glu Gln Ser Ile Pro Pro Ala Arg Val Lys
325 330 335
Gln
<210> 17
<211> 1035
<212> DNA
<213> 人工序列
<220>
<223> CBS5-M4DIE5 strep标签
<220>
<221> CDS
<222> (1)..(1035)
<400> 17
atg agg aag tac ggt ttg acc gtt gat aat gtt gtt gat gcc ttg atc 48
Met Arg Lys Tyr Gly Leu Thr Val Asp Asn Val Val Asp Ala Leu Ile
1 5 10 15
gtt gat gtt aac ggc aag ttg ttg aac aga tct act atg ggt gaa gat 96
Val Asp Val Asn Gly Lys Leu Leu Asn Arg Ser Thr Met Gly Glu Asp
20 25 30
ttg ttc tgg gct att aga ggt ggt ggt ggc ggt tct ttt ggt gtt att 144
Leu Phe Trp Ala Ile Arg Gly Gly Gly Gly Gly Ser Phe Gly Val Ile
35 40 45
ttg tct tgg aag atc aac ttg gtt gac gtc cca gaa att ttg act gtt 192
Leu Ser Trp Lys Ile Asn Leu Val Asp Val Pro Glu Ile Leu Thr Val
50 55 60
ttc aga gtt aac aag acc ttg gaa caa ggt ggt att gac gtc ttg tac 240
Phe Arg Val Asn Lys Thr Leu Glu Gln Gly Gly Ile Asp Val Leu Tyr
65 70 75 80
aaa tgg caa ttg gtg tcc tct aag ttg ttg gat ggt ttg ttt ttg aga 288
Lys Trp Gln Leu Val Ser Ser Lys Leu Leu Asp Gly Leu Phe Leu Arg
85 90 95
gcc ttg tct caa gtt gtc aac aga act atc acc gtt gaa ttt tac gct 336
Ala Leu Ser Gln Val Val Asn Arg Thr Ile Thr Val Glu Phe Tyr Ala
100 105 110
cag ttt ttg ggt aga gcc gat gaa ttg gtt gct atc gtt aat caa tct 384
Gln Phe Leu Gly Arg Ala Asp Glu Leu Val Ala Ile Val Asn Gln Ser
115 120 125
ttg cca gag ttg ggt ttg aag aga caa gat tgc ttg gaa atg tct tgg 432
Leu Pro Glu Leu Gly Leu Lys Arg Gln Asp Cys Leu Glu Met Ser Trp
130 135 140
ttg aac act acc ttg ttt tgg gaa gat ttg cca gtt ggt act cca act 480
Leu Asn Thr Thr Leu Phe Trp Glu Asp Leu Pro Val Gly Thr Pro Thr
145 150 155 160
tct gtt ttg ttg gat aga cca tct aag cca gag aag ttc ttc aag tct 528
Ser Val Leu Leu Asp Arg Pro Ser Lys Pro Glu Lys Phe Phe Lys Ser
165 170 175
aaa tcc gat tac gtc aag aag ccc att cca aaa gag ggt att aag aaa 576
Lys Ser Asp Tyr Val Lys Lys Pro Ile Pro Lys Glu Gly Ile Lys Lys
180 185 190
ttg tgg gaa gcc ttg ttg gag ttt aac aac aac aat atc gtc tac atg 624
Leu Trp Glu Ala Leu Leu Glu Phe Asn Asn Asn Asn Ile Val Tyr Met
195 200 205
cag tgg aat cca tac ggt ggt gtt atg gat act gtt cca gct gct gct 672
Gln Trp Asn Pro Tyr Gly Gly Val Met Asp Thr Val Pro Ala Ala Ala
210 215 220
act cca ttt cca cat aga aaa ggt aac ttg ttc gaa gtc cag tac tac 720
Thr Pro Phe Pro His Arg Lys Gly Asn Leu Phe Glu Val Gln Tyr Tyr
225 230 235 240
act tct tgg ttg gat gct aat gct aca atg ggt tct ttg gac atg atg 768
Thr Ser Trp Leu Asp Ala Asn Ala Thr Met Gly Ser Leu Asp Met Met
245 250 255
aag aag ttg tac gaa gtt gct gaa ccc tac gtt tct tct aat cca aga 816
Lys Lys Leu Tyr Glu Val Ala Glu Pro Tyr Val Ser Ser Asn Pro Arg
260 265 270
gaa gcc ttt ttg aac tac aga gac atc gac att ggt agc aat cca tct 864
Glu Ala Phe Leu Asn Tyr Arg Asp Ile Asp Ile Gly Ser Asn Pro Ser
275 280 285
aac gaa act aat gtt gaa gaa gcc gaa atc tac ggc tct aag tac ttt 912
Asn Glu Thr Asn Val Glu Glu Ala Glu Ile Tyr Gly Ser Lys Tyr Phe
290 295 300
gtt ggt aac ttg aag aga ttg atg gaa gtc aag gct aag tac gat cca 960
Val Gly Asn Leu Lys Arg Leu Met Glu Val Lys Ala Lys Tyr Asp Pro
305 310 315 320
gaa aac ttt ttc aag aac gag caa tct att cca cca gcc aga gtt aag 1008
Glu Asn Phe Phe Lys Asn Glu Gln Ser Ile Pro Pro Ala Arg Val Lys
325 330 335
caa tgg tca cat cca caa ttt gaa aag 1035
Gln Trp Ser His Pro Gln Phe Glu Lys
340 345
<210> 18
<211> 345
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 18
Met Arg Lys Tyr Gly Leu Thr Val Asp Asn Val Val Asp Ala Leu Ile
1 5 10 15
Val Asp Val Asn Gly Lys Leu Leu Asn Arg Ser Thr Met Gly Glu Asp
20 25 30
Leu Phe Trp Ala Ile Arg Gly Gly Gly Gly Gly Ser Phe Gly Val Ile
35 40 45
Leu Ser Trp Lys Ile Asn Leu Val Asp Val Pro Glu Ile Leu Thr Val
50 55 60
Phe Arg Val Asn Lys Thr Leu Glu Gln Gly Gly Ile Asp Val Leu Tyr
65 70 75 80
Lys Trp Gln Leu Val Ser Ser Lys Leu Leu Asp Gly Leu Phe Leu Arg
85 90 95
Ala Leu Ser Gln Val Val Asn Arg Thr Ile Thr Val Glu Phe Tyr Ala
100 105 110
Gln Phe Leu Gly Arg Ala Asp Glu Leu Val Ala Ile Val Asn Gln Ser
115 120 125
Leu Pro Glu Leu Gly Leu Lys Arg Gln Asp Cys Leu Glu Met Ser Trp
130 135 140
Leu Asn Thr Thr Leu Phe Trp Glu Asp Leu Pro Val Gly Thr Pro Thr
145 150 155 160
Ser Val Leu Leu Asp Arg Pro Ser Lys Pro Glu Lys Phe Phe Lys Ser
165 170 175
Lys Ser Asp Tyr Val Lys Lys Pro Ile Pro Lys Glu Gly Ile Lys Lys
180 185 190
Leu Trp Glu Ala Leu Leu Glu Phe Asn Asn Asn Asn Ile Val Tyr Met
195 200 205
Gln Trp Asn Pro Tyr Gly Gly Val Met Asp Thr Val Pro Ala Ala Ala
210 215 220
Thr Pro Phe Pro His Arg Lys Gly Asn Leu Phe Glu Val Gln Tyr Tyr
225 230 235 240
Thr Ser Trp Leu Asp Ala Asn Ala Thr Met Gly Ser Leu Asp Met Met
245 250 255
Lys Lys Leu Tyr Glu Val Ala Glu Pro Tyr Val Ser Ser Asn Pro Arg
260 265 270
Glu Ala Phe Leu Asn Tyr Arg Asp Ile Asp Ile Gly Ser Asn Pro Ser
275 280 285
Asn Glu Thr Asn Val Glu Glu Ala Glu Ile Tyr Gly Ser Lys Tyr Phe
290 295 300
Val Gly Asn Leu Lys Arg Leu Met Glu Val Lys Ala Lys Tyr Asp Pro
305 310 315 320
Glu Asn Phe Phe Lys Asn Glu Gln Ser Ile Pro Pro Ala Arg Val Lys
325 330 335
Gln Trp Ser His Pro Gln Phe Glu Lys
340 345
<210> 19
<211> 1092
<212> DNA
<213> 人工序列
<220>
<223> CBS5-M4DIE5-截断alpha因子strep标签
<400> 19
atgagatttc cttccatctt cacggctgtg ctatttgcag catcctccgc acttgcaatg 60
aggaagtacg gtttgaccgt tgataatgtt gttgatgcct tgatcgttga tgttaacggc 120
aagttgttga acagatctac tatgggtgaa gatttgttct gggctattag aggtggtggt 180
ggcggttctt ttggtgttat tttgtcttgg aagatcaact tggttgacgt cccagaaatt 240
ttgactgttt tcagagttaa caagaccttg gaacaaggtg gtattgacgt cttgtacaaa 300
tggcaattgg tgtcctctaa gttgttggat ggtttgtttt tgagagcctt gtctcaagtt 360
gtcaacagaa ctatcaccgt tgaattttac gctcagtttt tgggtagagc cgatgaattg 420
gttgctatcg ttaatcaatc tttgccagag ttgggtttga agagacaaga ttgcttggaa 480
atgtcttggt tgaacactac cttgttttgg gaagatttgc cagttggtac tccaacttct 540
gttttgttgg atagaccatc taagccagag aagttcttca agtctaaatc cgattacgtc 600
aagaagccca ttccaaaaga gggtattaag aaattgtggg aagccttgtt ggagtttaac 660
aacaacaata tcgtctacat gcagtggaat ccatacggtg gtgttatgga tactgttcca 720
gctgctgcta ctccatttcc acatagaaaa ggtaacttgt tcgaagtcca gtactacact 780
tcttggttgg atgctaatgc tacaatgggt tctttggaca tgatgaagaa gttgtacgaa 840
gttgctgaac cctacgtttc ttctaatcca agagaagcct ttttgaacta cagagacatc 900
gacattggta gcaatccatc taacgaaact aatgttgaag aagccgaaat ctacggctct 960
aagtactttg ttggtaactt gaagagattg atggaagtca aggctaagta cgatccagaa 1020
aactttttca agaacgagca atctattcca ccagccagag ttaagcaatg gtcacatcca 1080
caatttgaaa ag 1092
<210> 20
<211> 1302
<212> DNA
<213> 人工序列
<220>
<223> CBS5-M4DIE5-核苷酸-全长alpha因子strep标签
<220>
<221> CDS
<222> (1)..(1302)
<400> 20
atg aga ttt cct tca att ttt act gct gtt tta ttc gca gca tcc tcc 48
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser
1 5 10 15
gca tta gct gct cca gtc aac act aca aca gaa gat gaa acg gca caa 96
Ala Leu Ala Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln
20 25 30
att ccg gct gaa gct gtc atc ggt tac tca gat tta gaa ggg gat ttc 144
Ile Pro Ala Glu Ala Val Ile Gly Tyr Ser Asp Leu Glu Gly Asp Phe
35 40 45
gat gtt gct gtt ttg cca ttt tcc aac agc aca aat aac ggg tta ttg 192
Asp Val Ala Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu
50 55 60
ttt ata aat act act att gcc agc att gct gct aaa gaa gaa ggg gta 240
Phe Ile Asn Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val
65 70 75 80
tct ctc gag aaa aga gag gct gaa gct atg agg aag tac ggt ttg acc 288
Ser Leu Glu Lys Arg Glu Ala Glu Ala Met Arg Lys Tyr Gly Leu Thr
85 90 95
gtt gat aat gtt gtt gat gcc ttg atc gtt gat gtt aac ggc aag ttg 336
Val Asp Asn Val Val Asp Ala Leu Ile Val Asp Val Asn Gly Lys Leu
100 105 110
ttg aac aga tct act atg ggt gaa gat ttg ttc tgg gct att aga ggt 384
Leu Asn Arg Ser Thr Met Gly Glu Asp Leu Phe Trp Ala Ile Arg Gly
115 120 125
ggt ggt ggc ggt tct ttt ggt gtt att ttg tct tgg aag atc aac ttg 432
Gly Gly Gly Gly Ser Phe Gly Val Ile Leu Ser Trp Lys Ile Asn Leu
130 135 140
gtt gac gtc cca gaa att ttg act gtt ttc aga gtt aac aag acc ttg 480
Val Asp Val Pro Glu Ile Leu Thr Val Phe Arg Val Asn Lys Thr Leu
145 150 155 160
gaa caa ggt ggt att gac gtc ttg tac aaa tgg caa ttg gtg tcc tct 528
Glu Gln Gly Gly Ile Asp Val Leu Tyr Lys Trp Gln Leu Val Ser Ser
165 170 175
aag ttg ttg gat ggt ttg ttt ttg aga gcc ttg tct caa gtt gtc aac 576
Lys Leu Leu Asp Gly Leu Phe Leu Arg Ala Leu Ser Gln Val Val Asn
180 185 190
aga act atc acc gtt gaa ttt tac gct cag ttt ttg ggt aga gcc gat 624
Arg Thr Ile Thr Val Glu Phe Tyr Ala Gln Phe Leu Gly Arg Ala Asp
195 200 205
gaa ttg gtt gct atc gtt aat caa tct ttg cca gag ttg ggt ttg aag 672
Glu Leu Val Ala Ile Val Asn Gln Ser Leu Pro Glu Leu Gly Leu Lys
210 215 220
aga caa gat tgc ttg gaa atg tct tgg ttg aac act acc ttg ttt tgg 720
Arg Gln Asp Cys Leu Glu Met Ser Trp Leu Asn Thr Thr Leu Phe Trp
225 230 235 240
gaa gat ttg cca gtt ggt act cca act tct gtt ttg ttg gat aga cca 768
Glu Asp Leu Pro Val Gly Thr Pro Thr Ser Val Leu Leu Asp Arg Pro
245 250 255
tct aag cca gag aag ttc ttc aag tct aaa tcc gat tac gtc aag aag 816
Ser Lys Pro Glu Lys Phe Phe Lys Ser Lys Ser Asp Tyr Val Lys Lys
260 265 270
ccc att cca aaa gag ggt att aag aaa ttg tgg gaa gcc ttg ttg gag 864
Pro Ile Pro Lys Glu Gly Ile Lys Lys Leu Trp Glu Ala Leu Leu Glu
275 280 285
ttt aac aac aac aat atc gtc tac atg cag tgg aat cca tac ggt ggt 912
Phe Asn Asn Asn Asn Ile Val Tyr Met Gln Trp Asn Pro Tyr Gly Gly
290 295 300
gtt atg gat act gtt cca gct gct gct act cca ttt cca cat aga aaa 960
Val Met Asp Thr Val Pro Ala Ala Ala Thr Pro Phe Pro His Arg Lys
305 310 315 320
ggt aac ttg ttc gaa gtc cag tac tac act tct tgg ttg gat gct aat 1008
Gly Asn Leu Phe Glu Val Gln Tyr Tyr Thr Ser Trp Leu Asp Ala Asn
325 330 335
gct aca atg ggt tct ttg gac atg atg aag aag ttg tac gaa gtt gct 1056
Ala Thr Met Gly Ser Leu Asp Met Met Lys Lys Leu Tyr Glu Val Ala
340 345 350
gaa ccc tac gtt tct tct aat cca aga gaa gcc ttt ttg aac tac aga 1104
Glu Pro Tyr Val Ser Ser Asn Pro Arg Glu Ala Phe Leu Asn Tyr Arg
355 360 365
gac atc gac att ggt agc aat cca tct aac gaa act aat gtt gaa gaa 1152
Asp Ile Asp Ile Gly Ser Asn Pro Ser Asn Glu Thr Asn Val Glu Glu
370 375 380
gcc gaa atc tac ggc tct aag tac ttt gtt ggt aac ttg aag aga ttg 1200
Ala Glu Ile Tyr Gly Ser Lys Tyr Phe Val Gly Asn Leu Lys Arg Leu
385 390 395 400
atg gaa gtc aag gct aag tac gat cca gaa aac ttt ttc aag aac gag 1248
Met Glu Val Lys Ala Lys Tyr Asp Pro Glu Asn Phe Phe Lys Asn Glu
405 410 415
caa tct att cca cca gcc aga gtt aag caa tgg tca cat cca caa ttt 1296
Gln Ser Ile Pro Pro Ala Arg Val Lys Gln Trp Ser His Pro Gln Phe
420 425 430
gaa aag 1302
Glu Lys
<210> 21
<211> 434
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 21
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser
1 5 10 15
Ala Leu Ala Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln
20 25 30
Ile Pro Ala Glu Ala Val Ile Gly Tyr Ser Asp Leu Glu Gly Asp Phe
35 40 45
Asp Val Ala Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu
50 55 60
Phe Ile Asn Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val
65 70 75 80
Ser Leu Glu Lys Arg Glu Ala Glu Ala Met Arg Lys Tyr Gly Leu Thr
85 90 95
Val Asp Asn Val Val Asp Ala Leu Ile Val Asp Val Asn Gly Lys Leu
100 105 110
Leu Asn Arg Ser Thr Met Gly Glu Asp Leu Phe Trp Ala Ile Arg Gly
115 120 125
Gly Gly Gly Gly Ser Phe Gly Val Ile Leu Ser Trp Lys Ile Asn Leu
130 135 140
Val Asp Val Pro Glu Ile Leu Thr Val Phe Arg Val Asn Lys Thr Leu
145 150 155 160
Glu Gln Gly Gly Ile Asp Val Leu Tyr Lys Trp Gln Leu Val Ser Ser
165 170 175
Lys Leu Leu Asp Gly Leu Phe Leu Arg Ala Leu Ser Gln Val Val Asn
180 185 190
Arg Thr Ile Thr Val Glu Phe Tyr Ala Gln Phe Leu Gly Arg Ala Asp
195 200 205
Glu Leu Val Ala Ile Val Asn Gln Ser Leu Pro Glu Leu Gly Leu Lys
210 215 220
Arg Gln Asp Cys Leu Glu Met Ser Trp Leu Asn Thr Thr Leu Phe Trp
225 230 235 240
Glu Asp Leu Pro Val Gly Thr Pro Thr Ser Val Leu Leu Asp Arg Pro
245 250 255
Ser Lys Pro Glu Lys Phe Phe Lys Ser Lys Ser Asp Tyr Val Lys Lys
260 265 270
Pro Ile Pro Lys Glu Gly Ile Lys Lys Leu Trp Glu Ala Leu Leu Glu
275 280 285
Phe Asn Asn Asn Asn Ile Val Tyr Met Gln Trp Asn Pro Tyr Gly Gly
290 295 300
Val Met Asp Thr Val Pro Ala Ala Ala Thr Pro Phe Pro His Arg Lys
305 310 315 320
Gly Asn Leu Phe Glu Val Gln Tyr Tyr Thr Ser Trp Leu Asp Ala Asn
325 330 335
Ala Thr Met Gly Ser Leu Asp Met Met Lys Lys Leu Tyr Glu Val Ala
340 345 350
Glu Pro Tyr Val Ser Ser Asn Pro Arg Glu Ala Phe Leu Asn Tyr Arg
355 360 365
Asp Ile Asp Ile Gly Ser Asn Pro Ser Asn Glu Thr Asn Val Glu Glu
370 375 380
Ala Glu Ile Tyr Gly Ser Lys Tyr Phe Val Gly Asn Leu Lys Arg Leu
385 390 395 400
Met Glu Val Lys Ala Lys Tyr Asp Pro Glu Asn Phe Phe Lys Asn Glu
405 410 415
Gln Ser Ile Pro Pro Ala Arg Val Lys Gln Trp Ser His Pro Gln Phe
420 425 430
Glu Lys
<210> 22
<211> 1641
<212> DNA
<213> 人工序列
<220>
<223> CBS17-A0A1J6KPK0
<220>
<221> CDS
<222> (1)..(1641)
<400> 22
atg gcc tcc ttg aac tct atc atg gtg tct ttt ttc ttg gtc ctg ctg 48
Met Ala Ser Leu Asn Ser Ile Met Val Ser Phe Phe Leu Val Leu Leu
1 5 10 15
ttc tct tct tct tgt ttt gct ttg tct gat acc act cca gaa aag ttc 96
Phe Ser Ser Ser Cys Phe Ala Leu Ser Asp Thr Thr Pro Glu Lys Phe
20 25 30
tac caa tgt atc tgc aag aac tcc gat ttc tct gat cca ttt tct aag 144
Tyr Gln Cys Ile Cys Lys Asn Ser Asp Phe Ser Asp Pro Phe Ser Lys
35 40 45
gct ttc ttc act cca aac acc gat tct ttc aac acc gtt tta aac tct 192
Ala Phe Phe Thr Pro Asn Thr Asp Ser Phe Asn Thr Val Leu Asn Ser
50 55 60
acc gct caa aac ttg aga tgt ttg atg cca act gtt cca aag cca gtt 240
Thr Ala Gln Asn Leu Arg Cys Leu Met Pro Thr Val Pro Lys Pro Val
65 70 75 80
ttg att ttt act cca atg gcc gaa tct cat gtt caa gct gct gtt att 288
Leu Ile Phe Thr Pro Met Ala Glu Ser His Val Gln Ala Ala Val Ile
85 90 95
tgt gcc aag caa ttg aac ttg caa ttg aga gtt aga tcc ggt ggt cat 336
Cys Ala Lys Gln Leu Asn Leu Gln Leu Arg Val Arg Ser Gly Gly His
100 105 110
gat tat gaa ggt ttg tcc tac atc tcc gtt atg gat tct cca ttc gtt 384
Asp Tyr Glu Gly Leu Ser Tyr Ile Ser Val Met Asp Ser Pro Phe Val
115 120 125
atc gtt gac ttg tcc aag ttg aga gag att gac gtt aac atc gaa gaa 432
Ile Val Asp Leu Ser Lys Leu Arg Glu Ile Asp Val Asn Ile Glu Glu
130 135 140
aat tct gct tgg gct caa gct ggt gct act gtt ggt gaa gtt tat tac 480
Asn Ser Ala Trp Ala Gln Ala Gly Ala Thr Val Gly Glu Val Tyr Tyr
145 150 155 160
aga atc tcc gag aag tct aag act cat ggt ttt cca gct ggt ttg tgt 528
Arg Ile Ser Glu Lys Ser Lys Thr His Gly Phe Pro Ala Gly Leu Cys
165 170 175
acc tct tta ggt att ggt ggt cat atc act ggt ggt gct tat ggt tct 576
Thr Ser Leu Gly Ile Gly Gly His Ile Thr Gly Gly Ala Tyr Gly Ser
180 185 190
atg atg aga aaa ttt ggt ttg ggt gcc gat aac gtc att gat gct aga 624
Met Met Arg Lys Phe Gly Leu Gly Ala Asp Asn Val Ile Asp Ala Arg
195 200 205
atc gtt aat gcc aac ggt act atc ttg gac aga ttg tct atg ggt gaa 672
Ile Val Asn Ala Asn Gly Thr Ile Leu Asp Arg Leu Ser Met Gly Glu
210 215 220
gat ttg ttc tgg gct att aga ggt ggt ggt ggc gct tct ttt ggt att 720
Asp Leu Phe Trp Ala Ile Arg Gly Gly Gly Gly Ala Ser Phe Gly Ile
225 230 235 240
atc ttg tct tgg aag ttg aag ttg gtt acc gtt cca tct gtt gtt act 768
Ile Leu Ser Trp Lys Leu Lys Leu Val Thr Val Pro Ser Val Val Thr
245 250 255
gtt ttc acc gtt cca aaa acc ttg gaa gat ggt gct aca aag atc ttg 816
Val Phe Thr Val Pro Lys Thr Leu Glu Asp Gly Ala Thr Lys Ile Leu
260 265 270
tac aaa tgg caa caa gtt gcc gat aag atc gat gat gat ttg ttc atg 864
Tyr Lys Trp Gln Gln Val Ala Asp Lys Ile Asp Asp Asp Leu Phe Met
275 280 285
aga gtc gtt atc aac gtc gtt gac aaa aag gat aag aag ggt gaa aga 912
Arg Val Val Ile Asn Val Val Asp Lys Lys Asp Lys Lys Gly Glu Arg
290 295 300
act gtt caa acc gct tac aac tct ttg ttc ttg ggt aat gtt gac agg 960
Thr Val Gln Thr Ala Tyr Asn Ser Leu Phe Leu Gly Asn Val Asp Arg
305 310 315 320
ttg ttg cag atc atg aat gaa tct ttt cca gag ttg ggt ttg acc caa 1008
Leu Leu Gln Ile Met Asn Glu Ser Phe Pro Glu Leu Gly Leu Thr Gln
325 330 335
aaa gat tgc act gaa atg tcc tgg atc gag tcc atc ttg tat att gct 1056
Lys Asp Cys Thr Glu Met Ser Trp Ile Glu Ser Ile Leu Tyr Ile Ala
340 345 350
ggt ttc cca aca aaa acc cca cca gaa att ttg ttg caa ggt aag cca 1104
Gly Phe Pro Thr Lys Thr Pro Pro Glu Ile Leu Leu Gln Gly Lys Pro
355 360 365
act ttc aag aac tac ttc aag gct aag tcc gac ttc atc aaa gaa cct 1152
Thr Phe Lys Asn Tyr Phe Lys Ala Lys Ser Asp Phe Ile Lys Glu Pro
370 375 380
att cca gaa act ggt ctg gat ggt att tgg aaa aga cta ttg gaa gag 1200
Ile Pro Glu Thr Gly Leu Asp Gly Ile Trp Lys Arg Leu Leu Glu Glu
385 390 395 400
gac tcc cca ttg atg ata tgg aat cca tac ggt ggt atg atg gcc aac 1248
Asp Ser Pro Leu Met Ile Trp Asn Pro Tyr Gly Gly Met Met Ala Asn
405 410 415
att tct gaa tct gaa act cca ttt cca cac aga aag ggt gtt atc ttc 1296
Ile Ser Glu Ser Glu Thr Pro Phe Pro His Arg Lys Gly Val Ile Phe
420 425 430
aag atc caa tac ttg act ttg tgg acc gaa cca gat caa gaa ttg gct 1344
Lys Ile Gln Tyr Leu Thr Leu Trp Thr Glu Pro Asp Gln Glu Leu Ala
435 440 445
aca aaa cat gtt gac tgg atc aga aag ctg tac tct tac atg act tct 1392
Thr Lys His Val Asp Trp Ile Arg Lys Leu Tyr Ser Tyr Met Thr Ser
450 455 460
tac gct tct atg ttt cca aga gaa gcc tac gtt aac tac aga gat ttg 1440
Tyr Ala Ser Met Phe Pro Arg Glu Ala Tyr Val Asn Tyr Arg Asp Leu
465 470 475 480
gac ttg ggt atg aac aag aat ggc aac tct aac tcc tca ttc gtt caa 1488
Asp Leu Gly Met Asn Lys Asn Gly Asn Ser Asn Ser Ser Phe Val Gln
485 490 495
gct aca gtt tgg ggt aac aag tac ttc aac aac aat ttc aac agg ctg 1536
Ala Thr Val Trp Gly Asn Lys Tyr Phe Asn Asn Asn Phe Asn Arg Leu
500 505 510
gtc gaa atc aag act aag gtt gat cca gag aat ttc ttc aag ttc gaa 1584
Val Glu Ile Lys Thr Lys Val Asp Pro Glu Asn Phe Phe Lys Phe Glu
515 520 525
caa tcc att cca acc ttg cca ttg act aag aaa aga aag ggt aag aag 1632
Gln Ser Ile Pro Thr Leu Pro Leu Thr Lys Lys Arg Lys Gly Lys Lys
530 535 540
atc aac cat 1641
Ile Asn His
545
<210> 23
<211> 547
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 23
Met Ala Ser Leu Asn Ser Ile Met Val Ser Phe Phe Leu Val Leu Leu
1 5 10 15
Phe Ser Ser Ser Cys Phe Ala Leu Ser Asp Thr Thr Pro Glu Lys Phe
20 25 30
Tyr Gln Cys Ile Cys Lys Asn Ser Asp Phe Ser Asp Pro Phe Ser Lys
35 40 45
Ala Phe Phe Thr Pro Asn Thr Asp Ser Phe Asn Thr Val Leu Asn Ser
50 55 60
Thr Ala Gln Asn Leu Arg Cys Leu Met Pro Thr Val Pro Lys Pro Val
65 70 75 80
Leu Ile Phe Thr Pro Met Ala Glu Ser His Val Gln Ala Ala Val Ile
85 90 95
Cys Ala Lys Gln Leu Asn Leu Gln Leu Arg Val Arg Ser Gly Gly His
100 105 110
Asp Tyr Glu Gly Leu Ser Tyr Ile Ser Val Met Asp Ser Pro Phe Val
115 120 125
Ile Val Asp Leu Ser Lys Leu Arg Glu Ile Asp Val Asn Ile Glu Glu
130 135 140
Asn Ser Ala Trp Ala Gln Ala Gly Ala Thr Val Gly Glu Val Tyr Tyr
145 150 155 160
Arg Ile Ser Glu Lys Ser Lys Thr His Gly Phe Pro Ala Gly Leu Cys
165 170 175
Thr Ser Leu Gly Ile Gly Gly His Ile Thr Gly Gly Ala Tyr Gly Ser
180 185 190
Met Met Arg Lys Phe Gly Leu Gly Ala Asp Asn Val Ile Asp Ala Arg
195 200 205
Ile Val Asn Ala Asn Gly Thr Ile Leu Asp Arg Leu Ser Met Gly Glu
210 215 220
Asp Leu Phe Trp Ala Ile Arg Gly Gly Gly Gly Ala Ser Phe Gly Ile
225 230 235 240
Ile Leu Ser Trp Lys Leu Lys Leu Val Thr Val Pro Ser Val Val Thr
245 250 255
Val Phe Thr Val Pro Lys Thr Leu Glu Asp Gly Ala Thr Lys Ile Leu
260 265 270
Tyr Lys Trp Gln Gln Val Ala Asp Lys Ile Asp Asp Asp Leu Phe Met
275 280 285
Arg Val Val Ile Asn Val Val Asp Lys Lys Asp Lys Lys Gly Glu Arg
290 295 300
Thr Val Gln Thr Ala Tyr Asn Ser Leu Phe Leu Gly Asn Val Asp Arg
305 310 315 320
Leu Leu Gln Ile Met Asn Glu Ser Phe Pro Glu Leu Gly Leu Thr Gln
325 330 335
Lys Asp Cys Thr Glu Met Ser Trp Ile Glu Ser Ile Leu Tyr Ile Ala
340 345 350
Gly Phe Pro Thr Lys Thr Pro Pro Glu Ile Leu Leu Gln Gly Lys Pro
355 360 365
Thr Phe Lys Asn Tyr Phe Lys Ala Lys Ser Asp Phe Ile Lys Glu Pro
370 375 380
Ile Pro Glu Thr Gly Leu Asp Gly Ile Trp Lys Arg Leu Leu Glu Glu
385 390 395 400
Asp Ser Pro Leu Met Ile Trp Asn Pro Tyr Gly Gly Met Met Ala Asn
405 410 415
Ile Ser Glu Ser Glu Thr Pro Phe Pro His Arg Lys Gly Val Ile Phe
420 425 430
Lys Ile Gln Tyr Leu Thr Leu Trp Thr Glu Pro Asp Gln Glu Leu Ala
435 440 445
Thr Lys His Val Asp Trp Ile Arg Lys Leu Tyr Ser Tyr Met Thr Ser
450 455 460
Tyr Ala Ser Met Phe Pro Arg Glu Ala Tyr Val Asn Tyr Arg Asp Leu
465 470 475 480
Asp Leu Gly Met Asn Lys Asn Gly Asn Ser Asn Ser Ser Phe Val Gln
485 490 495
Ala Thr Val Trp Gly Asn Lys Tyr Phe Asn Asn Asn Phe Asn Arg Leu
500 505 510
Val Glu Ile Lys Thr Lys Val Asp Pro Glu Asn Phe Phe Lys Phe Glu
515 520 525
Gln Ser Ile Pro Thr Leu Pro Leu Thr Lys Lys Arg Lys Gly Lys Lys
530 535 540
Ile Asn His
545
<210> 24
<211> 1575
<212> DNA
<213> 人工序列
<220>
<223> CBS17-A0A1J6KPK0-无信号肽
<220>
<221> CDS
<222> (1)..(1575)
<400> 24
atg ttg tct gat acc act cca gaa aag ttc tac caa tgt atc tgc aag 48
Met Leu Ser Asp Thr Thr Pro Glu Lys Phe Tyr Gln Cys Ile Cys Lys
1 5 10 15
aac tcc gat ttc tct gat cca ttt tct aag gct ttc ttc act cca aac 96
Asn Ser Asp Phe Ser Asp Pro Phe Ser Lys Ala Phe Phe Thr Pro Asn
20 25 30
acc gat tct ttc aac acc gtt tta aac tct acc gct caa aac ttg aga 144
Thr Asp Ser Phe Asn Thr Val Leu Asn Ser Thr Ala Gln Asn Leu Arg
35 40 45
tgt ttg atg cca act gtt cca aag cca gtt ttg att ttt act cca atg 192
Cys Leu Met Pro Thr Val Pro Lys Pro Val Leu Ile Phe Thr Pro Met
50 55 60
gcc gaa tct cat gtt caa gct gct gtt att tgt gcc aag caa ttg aac 240
Ala Glu Ser His Val Gln Ala Ala Val Ile Cys Ala Lys Gln Leu Asn
65 70 75 80
ttg caa ttg aga gtt aga tcc ggt ggt cat gat tat gaa ggt ttg tcc 288
Leu Gln Leu Arg Val Arg Ser Gly Gly His Asp Tyr Glu Gly Leu Ser
85 90 95
tac atc tcc gtt atg gat tct cca ttc gtt atc gtt gac ttg tcc aag 336
Tyr Ile Ser Val Met Asp Ser Pro Phe Val Ile Val Asp Leu Ser Lys
100 105 110
ttg aga gag att gac gtt aac atc gaa gaa aat tct gct tgg gct caa 384
Leu Arg Glu Ile Asp Val Asn Ile Glu Glu Asn Ser Ala Trp Ala Gln
115 120 125
gct ggt gct act gtt ggt gaa gtt tat tac aga atc tcc gag aag tct 432
Ala Gly Ala Thr Val Gly Glu Val Tyr Tyr Arg Ile Ser Glu Lys Ser
130 135 140
aag act cat ggt ttt cca gct ggt ttg tgt acc tct tta ggt att ggt 480
Lys Thr His Gly Phe Pro Ala Gly Leu Cys Thr Ser Leu Gly Ile Gly
145 150 155 160
ggt cat atc act ggt ggt gct tat ggt tct atg atg aga aaa ttt ggt 528
Gly His Ile Thr Gly Gly Ala Tyr Gly Ser Met Met Arg Lys Phe Gly
165 170 175
ttg ggt gcc gat aac gtc att gat gct aga atc gtt aat gcc aac ggt 576
Leu Gly Ala Asp Asn Val Ile Asp Ala Arg Ile Val Asn Ala Asn Gly
180 185 190
act atc ttg gac aga ttg tct atg ggt gaa gat ttg ttc tgg gct att 624
Thr Ile Leu Asp Arg Leu Ser Met Gly Glu Asp Leu Phe Trp Ala Ile
195 200 205
aga ggt ggt ggt ggc gct tct ttt ggt att atc ttg tct tgg aag ttg 672
Arg Gly Gly Gly Gly Ala Ser Phe Gly Ile Ile Leu Ser Trp Lys Leu
210 215 220
aag ttg gtt acc gtt cca tct gtt gtt act gtt ttc acc gtt cca aaa 720
Lys Leu Val Thr Val Pro Ser Val Val Thr Val Phe Thr Val Pro Lys
225 230 235 240
acc ttg gaa gat ggt gct aca aag atc ttg tac aaa tgg caa caa gtt 768
Thr Leu Glu Asp Gly Ala Thr Lys Ile Leu Tyr Lys Trp Gln Gln Val
245 250 255
gcc gat aag atc gat gat gat ttg ttc atg aga gtc gtt atc aac gtc 816
Ala Asp Lys Ile Asp Asp Asp Leu Phe Met Arg Val Val Ile Asn Val
260 265 270
gtt gac aaa aag gat aag aag ggt gaa aga act gtt caa acc gct tac 864
Val Asp Lys Lys Asp Lys Lys Gly Glu Arg Thr Val Gln Thr Ala Tyr
275 280 285
aac tct ttg ttc ttg ggt aat gtt gac agg ttg ttg cag atc atg aat 912
Asn Ser Leu Phe Leu Gly Asn Val Asp Arg Leu Leu Gln Ile Met Asn
290 295 300
gaa tct ttt cca gag ttg ggt ttg acc caa aaa gat tgc act gaa atg 960
Glu Ser Phe Pro Glu Leu Gly Leu Thr Gln Lys Asp Cys Thr Glu Met
305 310 315 320
tcc tgg atc gag tcc atc ttg tat att gct ggt ttc cca aca aaa acc 1008
Ser Trp Ile Glu Ser Ile Leu Tyr Ile Ala Gly Phe Pro Thr Lys Thr
325 330 335
cca cca gaa att ttg ttg caa ggt aag cca act ttc aag aac tac ttc 1056
Pro Pro Glu Ile Leu Leu Gln Gly Lys Pro Thr Phe Lys Asn Tyr Phe
340 345 350
aag gct aag tcc gac ttc atc aaa gaa cct att cca gaa act ggt ctg 1104
Lys Ala Lys Ser Asp Phe Ile Lys Glu Pro Ile Pro Glu Thr Gly Leu
355 360 365
gat ggt att tgg aaa aga cta ttg gaa gag gac tcc cca ttg atg ata 1152
Asp Gly Ile Trp Lys Arg Leu Leu Glu Glu Asp Ser Pro Leu Met Ile
370 375 380
tgg aat cca tac ggt ggt atg atg gcc aac att tct gaa tct gaa act 1200
Trp Asn Pro Tyr Gly Gly Met Met Ala Asn Ile Ser Glu Ser Glu Thr
385 390 395 400
cca ttt cca cac aga aag ggt gtt atc ttc aag atc caa tac ttg act 1248
Pro Phe Pro His Arg Lys Gly Val Ile Phe Lys Ile Gln Tyr Leu Thr
405 410 415
ttg tgg acc gaa cca gat caa gaa ttg gct aca aaa cat gtt gac tgg 1296
Leu Trp Thr Glu Pro Asp Gln Glu Leu Ala Thr Lys His Val Asp Trp
420 425 430
atc aga aag ctg tac tct tac atg act tct tac gct tct atg ttt cca 1344
Ile Arg Lys Leu Tyr Ser Tyr Met Thr Ser Tyr Ala Ser Met Phe Pro
435 440 445
aga gaa gcc tac gtt aac tac aga gat ttg gac ttg ggt atg aac aag 1392
Arg Glu Ala Tyr Val Asn Tyr Arg Asp Leu Asp Leu Gly Met Asn Lys
450 455 460
aat ggc aac tct aac tcc tca ttc gtt caa gct aca gtt tgg ggt aac 1440
Asn Gly Asn Ser Asn Ser Ser Phe Val Gln Ala Thr Val Trp Gly Asn
465 470 475 480
aag tac ttc aac aac aat ttc aac agg ctg gtc gaa atc aag act aag 1488
Lys Tyr Phe Asn Asn Asn Phe Asn Arg Leu Val Glu Ile Lys Thr Lys
485 490 495
gtt gat cca gag aat ttc ttc aag ttc gaa caa tcc att cca acc ttg 1536
Val Asp Pro Glu Asn Phe Phe Lys Phe Glu Gln Ser Ile Pro Thr Leu
500 505 510
cca ttg act aag aaa aga aag ggt aag aag atc aac cat 1575
Pro Leu Thr Lys Lys Arg Lys Gly Lys Lys Ile Asn His
515 520 525
<210> 25
<211> 525
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 25
Met Leu Ser Asp Thr Thr Pro Glu Lys Phe Tyr Gln Cys Ile Cys Lys
1 5 10 15
Asn Ser Asp Phe Ser Asp Pro Phe Ser Lys Ala Phe Phe Thr Pro Asn
20 25 30
Thr Asp Ser Phe Asn Thr Val Leu Asn Ser Thr Ala Gln Asn Leu Arg
35 40 45
Cys Leu Met Pro Thr Val Pro Lys Pro Val Leu Ile Phe Thr Pro Met
50 55 60
Ala Glu Ser His Val Gln Ala Ala Val Ile Cys Ala Lys Gln Leu Asn
65 70 75 80
Leu Gln Leu Arg Val Arg Ser Gly Gly His Asp Tyr Glu Gly Leu Ser
85 90 95
Tyr Ile Ser Val Met Asp Ser Pro Phe Val Ile Val Asp Leu Ser Lys
100 105 110
Leu Arg Glu Ile Asp Val Asn Ile Glu Glu Asn Ser Ala Trp Ala Gln
115 120 125
Ala Gly Ala Thr Val Gly Glu Val Tyr Tyr Arg Ile Ser Glu Lys Ser
130 135 140
Lys Thr His Gly Phe Pro Ala Gly Leu Cys Thr Ser Leu Gly Ile Gly
145 150 155 160
Gly His Ile Thr Gly Gly Ala Tyr Gly Ser Met Met Arg Lys Phe Gly
165 170 175
Leu Gly Ala Asp Asn Val Ile Asp Ala Arg Ile Val Asn Ala Asn Gly
180 185 190
Thr Ile Leu Asp Arg Leu Ser Met Gly Glu Asp Leu Phe Trp Ala Ile
195 200 205
Arg Gly Gly Gly Gly Ala Ser Phe Gly Ile Ile Leu Ser Trp Lys Leu
210 215 220
Lys Leu Val Thr Val Pro Ser Val Val Thr Val Phe Thr Val Pro Lys
225 230 235 240
Thr Leu Glu Asp Gly Ala Thr Lys Ile Leu Tyr Lys Trp Gln Gln Val
245 250 255
Ala Asp Lys Ile Asp Asp Asp Leu Phe Met Arg Val Val Ile Asn Val
260 265 270
Val Asp Lys Lys Asp Lys Lys Gly Glu Arg Thr Val Gln Thr Ala Tyr
275 280 285
Asn Ser Leu Phe Leu Gly Asn Val Asp Arg Leu Leu Gln Ile Met Asn
290 295 300
Glu Ser Phe Pro Glu Leu Gly Leu Thr Gln Lys Asp Cys Thr Glu Met
305 310 315 320
Ser Trp Ile Glu Ser Ile Leu Tyr Ile Ala Gly Phe Pro Thr Lys Thr
325 330 335
Pro Pro Glu Ile Leu Leu Gln Gly Lys Pro Thr Phe Lys Asn Tyr Phe
340 345 350
Lys Ala Lys Ser Asp Phe Ile Lys Glu Pro Ile Pro Glu Thr Gly Leu
355 360 365
Asp Gly Ile Trp Lys Arg Leu Leu Glu Glu Asp Ser Pro Leu Met Ile
370 375 380
Trp Asn Pro Tyr Gly Gly Met Met Ala Asn Ile Ser Glu Ser Glu Thr
385 390 395 400
Pro Phe Pro His Arg Lys Gly Val Ile Phe Lys Ile Gln Tyr Leu Thr
405 410 415
Leu Trp Thr Glu Pro Asp Gln Glu Leu Ala Thr Lys His Val Asp Trp
420 425 430
Ile Arg Lys Leu Tyr Ser Tyr Met Thr Ser Tyr Ala Ser Met Phe Pro
435 440 445
Arg Glu Ala Tyr Val Asn Tyr Arg Asp Leu Asp Leu Gly Met Asn Lys
450 455 460
Asn Gly Asn Ser Asn Ser Ser Phe Val Gln Ala Thr Val Trp Gly Asn
465 470 475 480
Lys Tyr Phe Asn Asn Asn Phe Asn Arg Leu Val Glu Ile Lys Thr Lys
485 490 495
Val Asp Pro Glu Asn Phe Phe Lys Phe Glu Gln Ser Ile Pro Thr Leu
500 505 510
Pro Leu Thr Lys Lys Arg Lys Gly Lys Lys Ile Asn His
515 520 525
<210> 26
<211> 1599
<212> DNA
<213> 人工序列
<220>
<223> CBS17-A0A1J6KPK0-无信号肽-strep标签
<400> 26
atgttgtctg ataccactcc agaaaagttc taccaatgta tctgcaagaa ctccgatttc 60
tctgatccat tttctaaggc tttcttcact ccaaacaccg attctttcaa caccgtttta 120
aactctaccg ctcaaaactt gagatgtttg atgccaactg ttccaaagcc agttttgatt 180
tttactccaa tggccgaatc tcatgttcaa gctgctgtta tttgtgccaa gcaattgaac 240
ttgcaattga gagttagatc cggtggtcat gattatgaag gtttgtccta catctccgtt 300
atggattctc cattcgttat cgttgacttg tccaagttga gagagattga cgttaacatc 360
gaagaaaatt ctgcttgggc tcaagctggt gctactgttg gtgaagttta ttacagaatc 420
tccgagaagt ctaagactca tggttttcca gctggtttgt gtacctcttt aggtattggt 480
ggtcatatca ctggtggtgc ttatggttct atgatgagaa aatttggttt gggtgccgat 540
aacgtcattg atgctagaat cgttaatgcc aacggtacta tcttggacag attgtctatg 600
ggtgaagatt tgttctgggc tattagaggt ggtggtggcg cttcttttgg tattatcttg 660
tcttggaagt tgaagttggt taccgttcca tctgttgtta ctgttttcac cgttccaaaa 720
accttggaag atggtgctac aaagatcttg tacaaatggc aacaagttgc cgataagatc 780
gatgatgatt tgttcatgag agtcgttatc aacgtcgttg acaaaaagga taagaagggt 840
gaaagaactg ttcaaaccgc ttacaactct ttgttcttgg gtaatgttga caggttgttg 900
cagatcatga atgaatcttt tccagagttg ggtttgaccc aaaaagattg cactgaaatg 960
tcctggatcg agtccatctt gtatattgct ggtttcccaa caaaaacccc accagaaatt 1020
ttgttgcaag gtaagccaac tttcaagaac tacttcaagg ctaagtccga cttcatcaaa 1080
gaacctattc cagaaactgg tctggatggt atttggaaaa gactattgga agaggactcc 1140
ccattgatga tatggaatcc atacggtggt atgatggcca acatttctga atctgaaact 1200
ccatttccac acagaaaggg tgttatcttc aagatccaat acttgacttt gtggaccgaa 1260
ccagatcaag aattggctac aaaacatgtt gactggatca gaaagctgta ctcttacatg 1320
acttcttacg cttctatgtt tccaagagaa gcctacgtta actacagaga tttggacttg 1380
ggtatgaaca agaatggcaa ctctaactcc tcattcgttc aagctacagt ttggggtaac 1440
aagtacttca acaacaattt caacaggctg gtcgaaatca agactaaggt tgatccagag 1500
aatttcttca agttcgaaca atccattcca accttgccat tgactaagaa aagaaagggt 1560
aagaagatca accattggtc acatccacaa tttgaaaag 1599
<210> 27
<211> 1656
<212> DNA
<213> 人工序列
<220>
<223> CBS17-A0A1J6KPK0-截断alpha因子-无信号肽-strep标签
<400> 27
atgagatttc cttccatctt cacggctgtg ctatttgcag catcctccgc acttgcaatg 60
ttgtctgata ccactccaga aaagttctac caatgtatct gcaagaactc cgatttctct 120
gatccatttt ctaaggcttt cttcactcca aacaccgatt ctttcaacac cgttttaaac 180
tctaccgctc aaaacttgag atgtttgatg ccaactgttc caaagccagt tttgattttt 240
actccaatgg ccgaatctca tgttcaagct gctgttattt gtgccaagca attgaacttg 300
caattgagag ttagatccgg tggtcatgat tatgaaggtt tgtcctacat ctccgttatg 360
gattctccat tcgttatcgt tgacttgtcc aagttgagag agattgacgt taacatcgaa 420
gaaaattctg cttgggctca agctggtgct actgttggtg aagtttatta cagaatctcc 480
gagaagtcta agactcatgg ttttccagct ggtttgtgta cctctttagg tattggtggt 540
catatcactg gtggtgctta tggttctatg atgagaaaat ttggtttggg tgccgataac 600
gtcattgatg ctagaatcgt taatgccaac ggtactatct tggacagatt gtctatgggt 660
gaagatttgt tctgggctat tagaggtggt ggtggcgctt cttttggtat tatcttgtct 720
tggaagttga agttggttac cgttccatct gttgttactg ttttcaccgt tccaaaaacc 780
ttggaagatg gtgctacaaa gatcttgtac aaatggcaac aagttgccga taagatcgat 840
gatgatttgt tcatgagagt cgttatcaac gtcgttgaca aaaaggataa gaagggtgaa 900
agaactgttc aaaccgctta caactctttg ttcttgggta atgttgacag gttgttgcag 960
atcatgaatg aatcttttcc agagttgggt ttgacccaaa aagattgcac tgaaatgtcc 1020
tggatcgagt ccatcttgta tattgctggt ttcccaacaa aaaccccacc agaaattttg 1080
ttgcaaggta agccaacttt caagaactac ttcaaggcta agtccgactt catcaaagaa 1140
cctattccag aaactggtct ggatggtatt tggaaaagac tattggaaga ggactcccca 1200
ttgatgatat ggaatccata cggtggtatg atggccaaca tttctgaatc tgaaactcca 1260
tttccacaca gaaagggtgt tatcttcaag atccaatact tgactttgtg gaccgaacca 1320
gatcaagaat tggctacaaa acatgttgac tggatcagaa agctgtactc ttacatgact 1380
tcttacgctt ctatgtttcc aagagaagcc tacgttaact acagagattt ggacttgggt 1440
atgaacaaga atggcaactc taactcctca ttcgttcaag ctacagtttg gggtaacaag 1500
tacttcaaca acaatttcaa caggctggtc gaaatcaaga ctaaggttga tccagagaat 1560
ttcttcaagt tcgaacaatc cattccaacc ttgccattga ctaagaaaag aaagggtaag 1620
aagatcaacc attggtcaca tccacaattt gaaaag 1656
<210> 28
<211> 1866
<212> DNA
<213> 人工序列
<220>
<223> CBS17-A0A1J6KPK0-全长alpha因子-无信号肽-strep标签
<220>
<221> CDS
<222> (1)..(1866)
<400> 28
atg aga ttt cct tca att ttt act gct gtt tta ttc gca gca tcc tcc 48
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser
1 5 10 15
gca tta gct gct cca gtc aac act aca aca gaa gat gaa acg gca caa 96
Ala Leu Ala Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln
20 25 30
att ccg gct gaa gct gtc atc ggt tac tca gat tta gaa ggg gat ttc 144
Ile Pro Ala Glu Ala Val Ile Gly Tyr Ser Asp Leu Glu Gly Asp Phe
35 40 45
gat gtt gct gtt ttg cca ttt tcc aac agc aca aat aac ggg tta ttg 192
Asp Val Ala Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu
50 55 60
ttt ata aat act act att gcc agc att gct gct aaa gaa gaa ggg gta 240
Phe Ile Asn Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val
65 70 75 80
tct ctc gag aaa aga gag gct gaa gct atg ttg tct gat acc act cca 288
Ser Leu Glu Lys Arg Glu Ala Glu Ala Met Leu Ser Asp Thr Thr Pro
85 90 95
gaa aag ttc tac caa tgt atc tgc aag aac tcc gat ttc tct gat cca 336
Glu Lys Phe Tyr Gln Cys Ile Cys Lys Asn Ser Asp Phe Ser Asp Pro
100 105 110
ttt tct aag gct ttc ttc act cca aac acc gat tct ttc aac acc gtt 384
Phe Ser Lys Ala Phe Phe Thr Pro Asn Thr Asp Ser Phe Asn Thr Val
115 120 125
tta aac tct acc gct caa aac ttg aga tgt ttg atg cca act gtt cca 432
Leu Asn Ser Thr Ala Gln Asn Leu Arg Cys Leu Met Pro Thr Val Pro
130 135 140
aag cca gtt ttg att ttt act cca atg gcc gaa tct cat gtt caa gct 480
Lys Pro Val Leu Ile Phe Thr Pro Met Ala Glu Ser His Val Gln Ala
145 150 155 160
gct gtt att tgt gcc aag caa ttg aac ttg caa ttg aga gtt aga tcc 528
Ala Val Ile Cys Ala Lys Gln Leu Asn Leu Gln Leu Arg Val Arg Ser
165 170 175
ggt ggt cat gat tat gaa ggt ttg tcc tac atc tcc gtt atg gat tct 576
Gly Gly His Asp Tyr Glu Gly Leu Ser Tyr Ile Ser Val Met Asp Ser
180 185 190
cca ttc gtt atc gtt gac ttg tcc aag ttg aga gag att gac gtt aac 624
Pro Phe Val Ile Val Asp Leu Ser Lys Leu Arg Glu Ile Asp Val Asn
195 200 205
atc gaa gaa aat tct gct tgg gct caa gct ggt gct act gtt ggt gaa 672
Ile Glu Glu Asn Ser Ala Trp Ala Gln Ala Gly Ala Thr Val Gly Glu
210 215 220
gtt tat tac aga atc tcc gag aag tct aag act cat ggt ttt cca gct 720
Val Tyr Tyr Arg Ile Ser Glu Lys Ser Lys Thr His Gly Phe Pro Ala
225 230 235 240
ggt ttg tgt acc tct tta ggt att ggt ggt cat atc act ggt ggt gct 768
Gly Leu Cys Thr Ser Leu Gly Ile Gly Gly His Ile Thr Gly Gly Ala
245 250 255
tat ggt tct atg atg aga aaa ttt ggt ttg ggt gcc gat aac gtc att 816
Tyr Gly Ser Met Met Arg Lys Phe Gly Leu Gly Ala Asp Asn Val Ile
260 265 270
gat gct aga atc gtt aat gcc aac ggt act atc ttg gac aga ttg tct 864
Asp Ala Arg Ile Val Asn Ala Asn Gly Thr Ile Leu Asp Arg Leu Ser
275 280 285
atg ggt gaa gat ttg ttc tgg gct att aga ggt ggt ggt ggc gct tct 912
Met Gly Glu Asp Leu Phe Trp Ala Ile Arg Gly Gly Gly Gly Ala Ser
290 295 300
ttt ggt att atc ttg tct tgg aag ttg aag ttg gtt acc gtt cca tct 960
Phe Gly Ile Ile Leu Ser Trp Lys Leu Lys Leu Val Thr Val Pro Ser
305 310 315 320
gtt gtt act gtt ttc acc gtt cca aaa acc ttg gaa gat ggt gct aca 1008
Val Val Thr Val Phe Thr Val Pro Lys Thr Leu Glu Asp Gly Ala Thr
325 330 335
aag atc ttg tac aaa tgg caa caa gtt gcc gat aag atc gat gat gat 1056
Lys Ile Leu Tyr Lys Trp Gln Gln Val Ala Asp Lys Ile Asp Asp Asp
340 345 350
ttg ttc atg aga gtc gtt atc aac gtc gtt gac aaa aag gat aag aag 1104
Leu Phe Met Arg Val Val Ile Asn Val Val Asp Lys Lys Asp Lys Lys
355 360 365
ggt gaa aga act gtt caa acc gct tac aac tct ttg ttc ttg ggt aat 1152
Gly Glu Arg Thr Val Gln Thr Ala Tyr Asn Ser Leu Phe Leu Gly Asn
370 375 380
gtt gac agg ttg ttg cag atc atg aat gaa tct ttt cca gag ttg ggt 1200
Val Asp Arg Leu Leu Gln Ile Met Asn Glu Ser Phe Pro Glu Leu Gly
385 390 395 400
ttg acc caa aaa gat tgc act gaa atg tcc tgg atc gag tcc atc ttg 1248
Leu Thr Gln Lys Asp Cys Thr Glu Met Ser Trp Ile Glu Ser Ile Leu
405 410 415
tat att gct ggt ttc cca aca aaa acc cca cca gaa att ttg ttg caa 1296
Tyr Ile Ala Gly Phe Pro Thr Lys Thr Pro Pro Glu Ile Leu Leu Gln
420 425 430
ggt aag cca act ttc aag aac tac ttc aag gct aag tcc gac ttc atc 1344
Gly Lys Pro Thr Phe Lys Asn Tyr Phe Lys Ala Lys Ser Asp Phe Ile
435 440 445
aaa gaa cct att cca gaa act ggt ctg gat ggt att tgg aaa aga cta 1392
Lys Glu Pro Ile Pro Glu Thr Gly Leu Asp Gly Ile Trp Lys Arg Leu
450 455 460
ttg gaa gag gac tcc cca ttg atg ata tgg aat cca tac ggt ggt atg 1440
Leu Glu Glu Asp Ser Pro Leu Met Ile Trp Asn Pro Tyr Gly Gly Met
465 470 475 480
atg gcc aac att tct gaa tct gaa act cca ttt cca cac aga aag ggt 1488
Met Ala Asn Ile Ser Glu Ser Glu Thr Pro Phe Pro His Arg Lys Gly
485 490 495
gtt atc ttc aag atc caa tac ttg act ttg tgg acc gaa cca gat caa 1536
Val Ile Phe Lys Ile Gln Tyr Leu Thr Leu Trp Thr Glu Pro Asp Gln
500 505 510
gaa ttg gct aca aaa cat gtt gac tgg atc aga aag ctg tac tct tac 1584
Glu Leu Ala Thr Lys His Val Asp Trp Ile Arg Lys Leu Tyr Ser Tyr
515 520 525
atg act tct tac gct tct atg ttt cca aga gaa gcc tac gtt aac tac 1632
Met Thr Ser Tyr Ala Ser Met Phe Pro Arg Glu Ala Tyr Val Asn Tyr
530 535 540
aga gat ttg gac ttg ggt atg aac aag aat ggc aac tct aac tcc tca 1680
Arg Asp Leu Asp Leu Gly Met Asn Lys Asn Gly Asn Ser Asn Ser Ser
545 550 555 560
ttc gtt caa gct aca gtt tgg ggt aac aag tac ttc aac aac aat ttc 1728
Phe Val Gln Ala Thr Val Trp Gly Asn Lys Tyr Phe Asn Asn Asn Phe
565 570 575
aac agg ctg gtc gaa atc aag act aag gtt gat cca gag aat ttc ttc 1776
Asn Arg Leu Val Glu Ile Lys Thr Lys Val Asp Pro Glu Asn Phe Phe
580 585 590
aag ttc gaa caa tcc att cca acc ttg cca ttg act aag aaa aga aag 1824
Lys Phe Glu Gln Ser Ile Pro Thr Leu Pro Leu Thr Lys Lys Arg Lys
595 600 605
ggt aag aag atc aac cat tgg tca cat cca caa ttt gaa aag 1866
Gly Lys Lys Ile Asn His Trp Ser His Pro Gln Phe Glu Lys
610 615 620
<210> 29
<211> 622
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 29
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser
1 5 10 15
Ala Leu Ala Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln
20 25 30
Ile Pro Ala Glu Ala Val Ile Gly Tyr Ser Asp Leu Glu Gly Asp Phe
35 40 45
Asp Val Ala Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu
50 55 60
Phe Ile Asn Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val
65 70 75 80
Ser Leu Glu Lys Arg Glu Ala Glu Ala Met Leu Ser Asp Thr Thr Pro
85 90 95
Glu Lys Phe Tyr Gln Cys Ile Cys Lys Asn Ser Asp Phe Ser Asp Pro
100 105 110
Phe Ser Lys Ala Phe Phe Thr Pro Asn Thr Asp Ser Phe Asn Thr Val
115 120 125
Leu Asn Ser Thr Ala Gln Asn Leu Arg Cys Leu Met Pro Thr Val Pro
130 135 140
Lys Pro Val Leu Ile Phe Thr Pro Met Ala Glu Ser His Val Gln Ala
145 150 155 160
Ala Val Ile Cys Ala Lys Gln Leu Asn Leu Gln Leu Arg Val Arg Ser
165 170 175
Gly Gly His Asp Tyr Glu Gly Leu Ser Tyr Ile Ser Val Met Asp Ser
180 185 190
Pro Phe Val Ile Val Asp Leu Ser Lys Leu Arg Glu Ile Asp Val Asn
195 200 205
Ile Glu Glu Asn Ser Ala Trp Ala Gln Ala Gly Ala Thr Val Gly Glu
210 215 220
Val Tyr Tyr Arg Ile Ser Glu Lys Ser Lys Thr His Gly Phe Pro Ala
225 230 235 240
Gly Leu Cys Thr Ser Leu Gly Ile Gly Gly His Ile Thr Gly Gly Ala
245 250 255
Tyr Gly Ser Met Met Arg Lys Phe Gly Leu Gly Ala Asp Asn Val Ile
260 265 270
Asp Ala Arg Ile Val Asn Ala Asn Gly Thr Ile Leu Asp Arg Leu Ser
275 280 285
Met Gly Glu Asp Leu Phe Trp Ala Ile Arg Gly Gly Gly Gly Ala Ser
290 295 300
Phe Gly Ile Ile Leu Ser Trp Lys Leu Lys Leu Val Thr Val Pro Ser
305 310 315 320
Val Val Thr Val Phe Thr Val Pro Lys Thr Leu Glu Asp Gly Ala Thr
325 330 335
Lys Ile Leu Tyr Lys Trp Gln Gln Val Ala Asp Lys Ile Asp Asp Asp
340 345 350
Leu Phe Met Arg Val Val Ile Asn Val Val Asp Lys Lys Asp Lys Lys
355 360 365
Gly Glu Arg Thr Val Gln Thr Ala Tyr Asn Ser Leu Phe Leu Gly Asn
370 375 380
Val Asp Arg Leu Leu Gln Ile Met Asn Glu Ser Phe Pro Glu Leu Gly
385 390 395 400
Leu Thr Gln Lys Asp Cys Thr Glu Met Ser Trp Ile Glu Ser Ile Leu
405 410 415
Tyr Ile Ala Gly Phe Pro Thr Lys Thr Pro Pro Glu Ile Leu Leu Gln
420 425 430
Gly Lys Pro Thr Phe Lys Asn Tyr Phe Lys Ala Lys Ser Asp Phe Ile
435 440 445
Lys Glu Pro Ile Pro Glu Thr Gly Leu Asp Gly Ile Trp Lys Arg Leu
450 455 460
Leu Glu Glu Asp Ser Pro Leu Met Ile Trp Asn Pro Tyr Gly Gly Met
465 470 475 480
Met Ala Asn Ile Ser Glu Ser Glu Thr Pro Phe Pro His Arg Lys Gly
485 490 495
Val Ile Phe Lys Ile Gln Tyr Leu Thr Leu Trp Thr Glu Pro Asp Gln
500 505 510
Glu Leu Ala Thr Lys His Val Asp Trp Ile Arg Lys Leu Tyr Ser Tyr
515 520 525
Met Thr Ser Tyr Ala Ser Met Phe Pro Arg Glu Ala Tyr Val Asn Tyr
530 535 540
Arg Asp Leu Asp Leu Gly Met Asn Lys Asn Gly Asn Ser Asn Ser Ser
545 550 555 560
Phe Val Gln Ala Thr Val Trp Gly Asn Lys Tyr Phe Asn Asn Asn Phe
565 570 575
Asn Arg Leu Val Glu Ile Lys Thr Lys Val Asp Pro Glu Asn Phe Phe
580 585 590
Lys Phe Glu Gln Ser Ile Pro Thr Leu Pro Leu Thr Lys Lys Arg Lys
595 600 605
Gly Lys Lys Ile Asn His Trp Ser His Pro Gln Phe Glu Lys
610 615 620
<210> 30
<211> 1521
<212> DNA
<213> 人工序列
<220>
<223> CBS35-M5X864
<400> 30
atgcagatca ttatcaccaa gagatcctct gcttacttct ctgctgttca atcctctatc 60
caaaacttga tcttcgttac cagaggtact tctagaccag aagctattat taccttgttg 120
catgattctc acggtgtctt gattagaatc agatctagag gtcatgacta cgaaggtcaa 180
tcttatgttg ctagagcccc attcgttatc atcgatttgt ttaacttgag gtccatcgac 240
gttgacattg aaaatggttc tgcttgggtt gaatctggtg ctactttggg tgagttgtat 300
tacagaattg ccgaaaagtc taaggcttac ggttttccag ctggttcttg tccaactatt 360
ggtgttggtg gtcatatttc tggtggtggt tttggtactt tgttcagaaa gtatggtttg 420
gctgctgata acgttttgga tgctaaaatc gttgatgtca acggtagagt tttggacaga 480
aaacctatgg gtgaagaatt attctgggct attagaggtg gtggtggatc ttcttttggt 540
gttattttgg cttggaagtt gaagttggtt ccagttccac catctgttac tagacatgaa 600
gatttgttct tgcactccgt tataggtgtt ggtaacaaag ctggtactaa tggtgataag 660
accattatta tcgagttcag ctccttgttt ttgggtccag ttgaaaagtt gttgccatcc 720
atgcaagata acttctccga attgtctgtt gatagatccg attgcactga aatgtcctgg 780
attgaatctg tcttgtactt cgctttcatc ccaagaaatg aatctgaagc cttgttgggt 840
agaacccaac aatctaaatc tttcgctaag ccacaaggta ttttctggac tcatagaact 900
ccaaccatga acgttaattc tccaacctct aagttctgta ctacttgtac tccatctaag 960
acctacaaga gaggtacatc tccaaagatt gaagttactg gtactttcgg cactttgaaa 1020
acttctacct ctgttttcaa ggccaagtcc gattatgtta acgaacctat ttctaaggct 1080
ggtttggaag gtttgtggca aactttgata gaagttaagg cctacctgat cttgacatta 1140
tacggtggta agatctccga gatttccaac tctgaaattc atttcccaca caggtctggt 1200
aacttgttca aaattcagta catggttacc tgggacgact acagagaaaa cgaaattggt 1260
gaatccctga tgagaaagtt gtacgattat atggctccct acgtttctaa atctccaaga 1320
gctgcttatc tgaactacaa ggatttggac ttaggtagaa acaacgatgt tcatacttct 1380
tacgctcaag ctagtatttg gggcttgaag tactttaaga acaacttcag aagattggtc 1440
cacgttaaga ctttggttga tccaggtaat ttcttcaggg acgaacaatc tattccatcc 1500
gttagatctt cccgtaagaa g 1521
<210> 31
<211> 1545
<212> DNA
<213> 人工序列
<220>
<223> CBS35-M5X864-strep标签
<220>
<221> CDS
<222> (1)..(1545)
<400> 31
atg cag atc att atc acc aag aga tcc tct gct tac ttc tct gct gtt 48
Met Gln Ile Ile Ile Thr Lys Arg Ser Ser Ala Tyr Phe Ser Ala Val
1 5 10 15
caa tcc tct atc caa aac ttg atc ttc gtt acc aga ggt act tct aga 96
Gln Ser Ser Ile Gln Asn Leu Ile Phe Val Thr Arg Gly Thr Ser Arg
20 25 30
cca gaa gct att att acc ttg ttg cat gat tct cac ggt gtc ttg att 144
Pro Glu Ala Ile Ile Thr Leu Leu His Asp Ser His Gly Val Leu Ile
35 40 45
aga atc aga tct aga ggt cat gac tac gaa ggt caa tct tat gtt gct 192
Arg Ile Arg Ser Arg Gly His Asp Tyr Glu Gly Gln Ser Tyr Val Ala
50 55 60
aga gcc cca ttc gtt atc atc gat ttg ttt aac ttg agg tcc atc gac 240
Arg Ala Pro Phe Val Ile Ile Asp Leu Phe Asn Leu Arg Ser Ile Asp
65 70 75 80
gtt gac att gaa aat ggt tct gct tgg gtt gaa tct ggt gct act ttg 288
Val Asp Ile Glu Asn Gly Ser Ala Trp Val Glu Ser Gly Ala Thr Leu
85 90 95
ggt gag ttg tat tac aga att gcc gaa aag tct aag gct tac ggt ttt 336
Gly Glu Leu Tyr Tyr Arg Ile Ala Glu Lys Ser Lys Ala Tyr Gly Phe
100 105 110
cca gct ggt tct tgt cca act att ggt gtt ggt ggt cat att tct ggt 384
Pro Ala Gly Ser Cys Pro Thr Ile Gly Val Gly Gly His Ile Ser Gly
115 120 125
ggt ggt ttt ggt act ttg ttc aga aag tat ggt ttg gct gct gat aac 432
Gly Gly Phe Gly Thr Leu Phe Arg Lys Tyr Gly Leu Ala Ala Asp Asn
130 135 140
gtt ttg gat gct aaa atc gtt gat gtc aac ggt aga gtt ttg gac aga 480
Val Leu Asp Ala Lys Ile Val Asp Val Asn Gly Arg Val Leu Asp Arg
145 150 155 160
aaa cct atg ggt gaa gaa tta ttc tgg gct att aga ggt ggt ggt gga 528
Lys Pro Met Gly Glu Glu Leu Phe Trp Ala Ile Arg Gly Gly Gly Gly
165 170 175
tct tct ttt ggt gtt att ttg gct tgg aag ttg aag ttg gtt cca gtt 576
Ser Ser Phe Gly Val Ile Leu Ala Trp Lys Leu Lys Leu Val Pro Val
180 185 190
cca cca tct gtt act aga cat gaa gat ttg ttc ttg cac tcc gtt ata 624
Pro Pro Ser Val Thr Arg His Glu Asp Leu Phe Leu His Ser Val Ile
195 200 205
ggt gtt ggt aac aaa gct ggt act aat ggt gat aag acc att att atc 672
Gly Val Gly Asn Lys Ala Gly Thr Asn Gly Asp Lys Thr Ile Ile Ile
210 215 220
gag ttc agc tcc ttg ttt ttg ggt cca gtt gaa aag ttg ttg cca tcc 720
Glu Phe Ser Ser Leu Phe Leu Gly Pro Val Glu Lys Leu Leu Pro Ser
225 230 235 240
atg caa gat aac ttc tcc gaa ttg tct gtt gat aga tcc gat tgc act 768
Met Gln Asp Asn Phe Ser Glu Leu Ser Val Asp Arg Ser Asp Cys Thr
245 250 255
gaa atg tcc tgg att gaa tct gtc ttg tac ttc gct ttc atc cca aga 816
Glu Met Ser Trp Ile Glu Ser Val Leu Tyr Phe Ala Phe Ile Pro Arg
260 265 270
aat gaa tct gaa gcc ttg ttg ggt aga acc caa caa tct aaa tct ttc 864
Asn Glu Ser Glu Ala Leu Leu Gly Arg Thr Gln Gln Ser Lys Ser Phe
275 280 285
gct aag cca caa ggt att ttc tgg act cat aga act cca acc atg aac 912
Ala Lys Pro Gln Gly Ile Phe Trp Thr His Arg Thr Pro Thr Met Asn
290 295 300
gtt aat tct cca acc tct aag ttc tgt act act tgt act cca tct aag 960
Val Asn Ser Pro Thr Ser Lys Phe Cys Thr Thr Cys Thr Pro Ser Lys
305 310 315 320
acc tac aag aga ggt aca tct cca aag att gaa gtt act ggt act ttc 1008
Thr Tyr Lys Arg Gly Thr Ser Pro Lys Ile Glu Val Thr Gly Thr Phe
325 330 335
ggc act ttg aaa act tct acc tct gtt ttc aag gcc aag tcc gat tat 1056
Gly Thr Leu Lys Thr Ser Thr Ser Val Phe Lys Ala Lys Ser Asp Tyr
340 345 350
gtt aac gaa cct att tct aag gct ggt ttg gaa ggt ttg tgg caa act 1104
Val Asn Glu Pro Ile Ser Lys Ala Gly Leu Glu Gly Leu Trp Gln Thr
355 360 365
ttg ata gaa gtt aag gcc tac ctg atc ttg aca tta tac ggt ggt aag 1152
Leu Ile Glu Val Lys Ala Tyr Leu Ile Leu Thr Leu Tyr Gly Gly Lys
370 375 380
atc tcc gag att tcc aac tct gaa att cat ttc cca cac agg tct ggt 1200
Ile Ser Glu Ile Ser Asn Ser Glu Ile His Phe Pro His Arg Ser Gly
385 390 395 400
aac ttg ttc aaa att cag tac atg gtt acc tgg gac gac tac aga gaa 1248
Asn Leu Phe Lys Ile Gln Tyr Met Val Thr Trp Asp Asp Tyr Arg Glu
405 410 415
aac gaa att ggt gaa tcc ctg atg aga aag ttg tac gat tat atg gct 1296
Asn Glu Ile Gly Glu Ser Leu Met Arg Lys Leu Tyr Asp Tyr Met Ala
420 425 430
ccc tac gtt tct aaa tct cca aga gct gct tat ctg aac tac aag gat 1344
Pro Tyr Val Ser Lys Ser Pro Arg Ala Ala Tyr Leu Asn Tyr Lys Asp
435 440 445
ttg gac tta ggt aga aac aac gat gtt cat act tct tac gct caa gct 1392
Leu Asp Leu Gly Arg Asn Asn Asp Val His Thr Ser Tyr Ala Gln Ala
450 455 460
agt att tgg ggc ttg aag tac ttt aag aac aac ttc aga aga ttg gtc 1440
Ser Ile Trp Gly Leu Lys Tyr Phe Lys Asn Asn Phe Arg Arg Leu Val
465 470 475 480
cac gtt aag act ttg gtt gat cca ggt aat ttc ttc agg gac gaa caa 1488
His Val Lys Thr Leu Val Asp Pro Gly Asn Phe Phe Arg Asp Glu Gln
485 490 495
tct att cca tcc gtt aga tct tcc cgt aag aag tgg tca cat cca caa 1536
Ser Ile Pro Ser Val Arg Ser Ser Arg Lys Lys Trp Ser His Pro Gln
500 505 510
ttt gaa aag 1545
Phe Glu Lys
515
<210> 32
<211> 515
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 32
Met Gln Ile Ile Ile Thr Lys Arg Ser Ser Ala Tyr Phe Ser Ala Val
1 5 10 15
Gln Ser Ser Ile Gln Asn Leu Ile Phe Val Thr Arg Gly Thr Ser Arg
20 25 30
Pro Glu Ala Ile Ile Thr Leu Leu His Asp Ser His Gly Val Leu Ile
35 40 45
Arg Ile Arg Ser Arg Gly His Asp Tyr Glu Gly Gln Ser Tyr Val Ala
50 55 60
Arg Ala Pro Phe Val Ile Ile Asp Leu Phe Asn Leu Arg Ser Ile Asp
65 70 75 80
Val Asp Ile Glu Asn Gly Ser Ala Trp Val Glu Ser Gly Ala Thr Leu
85 90 95
Gly Glu Leu Tyr Tyr Arg Ile Ala Glu Lys Ser Lys Ala Tyr Gly Phe
100 105 110
Pro Ala Gly Ser Cys Pro Thr Ile Gly Val Gly Gly His Ile Ser Gly
115 120 125
Gly Gly Phe Gly Thr Leu Phe Arg Lys Tyr Gly Leu Ala Ala Asp Asn
130 135 140
Val Leu Asp Ala Lys Ile Val Asp Val Asn Gly Arg Val Leu Asp Arg
145 150 155 160
Lys Pro Met Gly Glu Glu Leu Phe Trp Ala Ile Arg Gly Gly Gly Gly
165 170 175
Ser Ser Phe Gly Val Ile Leu Ala Trp Lys Leu Lys Leu Val Pro Val
180 185 190
Pro Pro Ser Val Thr Arg His Glu Asp Leu Phe Leu His Ser Val Ile
195 200 205
Gly Val Gly Asn Lys Ala Gly Thr Asn Gly Asp Lys Thr Ile Ile Ile
210 215 220
Glu Phe Ser Ser Leu Phe Leu Gly Pro Val Glu Lys Leu Leu Pro Ser
225 230 235 240
Met Gln Asp Asn Phe Ser Glu Leu Ser Val Asp Arg Ser Asp Cys Thr
245 250 255
Glu Met Ser Trp Ile Glu Ser Val Leu Tyr Phe Ala Phe Ile Pro Arg
260 265 270
Asn Glu Ser Glu Ala Leu Leu Gly Arg Thr Gln Gln Ser Lys Ser Phe
275 280 285
Ala Lys Pro Gln Gly Ile Phe Trp Thr His Arg Thr Pro Thr Met Asn
290 295 300
Val Asn Ser Pro Thr Ser Lys Phe Cys Thr Thr Cys Thr Pro Ser Lys
305 310 315 320
Thr Tyr Lys Arg Gly Thr Ser Pro Lys Ile Glu Val Thr Gly Thr Phe
325 330 335
Gly Thr Leu Lys Thr Ser Thr Ser Val Phe Lys Ala Lys Ser Asp Tyr
340 345 350
Val Asn Glu Pro Ile Ser Lys Ala Gly Leu Glu Gly Leu Trp Gln Thr
355 360 365
Leu Ile Glu Val Lys Ala Tyr Leu Ile Leu Thr Leu Tyr Gly Gly Lys
370 375 380
Ile Ser Glu Ile Ser Asn Ser Glu Ile His Phe Pro His Arg Ser Gly
385 390 395 400
Asn Leu Phe Lys Ile Gln Tyr Met Val Thr Trp Asp Asp Tyr Arg Glu
405 410 415
Asn Glu Ile Gly Glu Ser Leu Met Arg Lys Leu Tyr Asp Tyr Met Ala
420 425 430
Pro Tyr Val Ser Lys Ser Pro Arg Ala Ala Tyr Leu Asn Tyr Lys Asp
435 440 445
Leu Asp Leu Gly Arg Asn Asn Asp Val His Thr Ser Tyr Ala Gln Ala
450 455 460
Ser Ile Trp Gly Leu Lys Tyr Phe Lys Asn Asn Phe Arg Arg Leu Val
465 470 475 480
His Val Lys Thr Leu Val Asp Pro Gly Asn Phe Phe Arg Asp Glu Gln
485 490 495
Ser Ile Pro Ser Val Arg Ser Ser Arg Lys Lys Trp Ser His Pro Gln
500 505 510
Phe Glu Lys
515
<210> 33
<211> 1602
<212> DNA
<213> 人工序列
<220>
<223> CBS35-M5X864-截断alpha因子-strep标签
<220>
<221> CDS
<222> (1)..(1602)
<400> 33
atg aga ttt cct tcc atc ttc acg gct gtg cta ttt gca gca tcc tcc 48
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser
1 5 10 15
gca ctt gca atg cag atc att atc acc aag aga tcc tct gct tac ttc 96
Ala Leu Ala Met Gln Ile Ile Ile Thr Lys Arg Ser Ser Ala Tyr Phe
20 25 30
tct gct gtt caa tcc tct atc caa aac ttg atc ttc gtt acc aga ggt 144
Ser Ala Val Gln Ser Ser Ile Gln Asn Leu Ile Phe Val Thr Arg Gly
35 40 45
act tct aga cca gaa gct att att acc ttg ttg cat gat tct cac ggt 192
Thr Ser Arg Pro Glu Ala Ile Ile Thr Leu Leu His Asp Ser His Gly
50 55 60
gtc ttg att aga atc aga tct aga ggt cat gac tac gaa ggt caa tct 240
Val Leu Ile Arg Ile Arg Ser Arg Gly His Asp Tyr Glu Gly Gln Ser
65 70 75 80
tat gtt gct aga gcc cca ttc gtt atc atc gat ttg ttt aac ttg agg 288
Tyr Val Ala Arg Ala Pro Phe Val Ile Ile Asp Leu Phe Asn Leu Arg
85 90 95
tcc atc gac gtt gac att gaa aat ggt tct gct tgg gtt gaa tct ggt 336
Ser Ile Asp Val Asp Ile Glu Asn Gly Ser Ala Trp Val Glu Ser Gly
100 105 110
gct act ttg ggt gag ttg tat tac aga att gcc gaa aag tct aag gct 384
Ala Thr Leu Gly Glu Leu Tyr Tyr Arg Ile Ala Glu Lys Ser Lys Ala
115 120 125
tac ggt ttt cca gct ggt tct tgt cca act att ggt gtt ggt ggt cat 432
Tyr Gly Phe Pro Ala Gly Ser Cys Pro Thr Ile Gly Val Gly Gly His
130 135 140
att tct ggt ggt ggt ttt ggt act ttg ttc aga aag tat ggt ttg gct 480
Ile Ser Gly Gly Gly Phe Gly Thr Leu Phe Arg Lys Tyr Gly Leu Ala
145 150 155 160
gct gat aac gtt ttg gat gct aaa atc gtt gat gtc aac ggt aga gtt 528
Ala Asp Asn Val Leu Asp Ala Lys Ile Val Asp Val Asn Gly Arg Val
165 170 175
ttg gac aga aaa cct atg ggt gaa gaa tta ttc tgg gct att aga ggt 576
Leu Asp Arg Lys Pro Met Gly Glu Glu Leu Phe Trp Ala Ile Arg Gly
180 185 190
ggt ggt gga tct tct ttt ggt gtt att ttg gct tgg aag ttg aag ttg 624
Gly Gly Gly Ser Ser Phe Gly Val Ile Leu Ala Trp Lys Leu Lys Leu
195 200 205
gtt cca gtt cca cca tct gtt act aga cat gaa gat ttg ttc ttg cac 672
Val Pro Val Pro Pro Ser Val Thr Arg His Glu Asp Leu Phe Leu His
210 215 220
tcc gtt ata ggt gtt ggt aac aaa gct ggt act aat ggt gat aag acc 720
Ser Val Ile Gly Val Gly Asn Lys Ala Gly Thr Asn Gly Asp Lys Thr
225 230 235 240
att att atc gag ttc agc tcc ttg ttt ttg ggt cca gtt gaa aag ttg 768
Ile Ile Ile Glu Phe Ser Ser Leu Phe Leu Gly Pro Val Glu Lys Leu
245 250 255
ttg cca tcc atg caa gat aac ttc tcc gaa ttg tct gtt gat aga tcc 816
Leu Pro Ser Met Gln Asp Asn Phe Ser Glu Leu Ser Val Asp Arg Ser
260 265 270
gat tgc act gaa atg tcc tgg att gaa tct gtc ttg tac ttc gct ttc 864
Asp Cys Thr Glu Met Ser Trp Ile Glu Ser Val Leu Tyr Phe Ala Phe
275 280 285
atc cca aga aat gaa tct gaa gcc ttg ttg ggt aga acc caa caa tct 912
Ile Pro Arg Asn Glu Ser Glu Ala Leu Leu Gly Arg Thr Gln Gln Ser
290 295 300
aaa tct ttc gct aag cca caa ggt att ttc tgg act cat aga act cca 960
Lys Ser Phe Ala Lys Pro Gln Gly Ile Phe Trp Thr His Arg Thr Pro
305 310 315 320
acc atg aac gtt aat tct cca acc tct aag ttc tgt act act tgt act 1008
Thr Met Asn Val Asn Ser Pro Thr Ser Lys Phe Cys Thr Thr Cys Thr
325 330 335
cca tct aag acc tac aag aga ggt aca tct cca aag att gaa gtt act 1056
Pro Ser Lys Thr Tyr Lys Arg Gly Thr Ser Pro Lys Ile Glu Val Thr
340 345 350
ggt act ttc ggc act ttg aaa act tct acc tct gtt ttc aag gcc aag 1104
Gly Thr Phe Gly Thr Leu Lys Thr Ser Thr Ser Val Phe Lys Ala Lys
355 360 365
tcc gat tat gtt aac gaa cct att tct aag gct ggt ttg gaa ggt ttg 1152
Ser Asp Tyr Val Asn Glu Pro Ile Ser Lys Ala Gly Leu Glu Gly Leu
370 375 380
tgg caa act ttg ata gaa gtt aag gcc tac ctg atc ttg aca tta tac 1200
Trp Gln Thr Leu Ile Glu Val Lys Ala Tyr Leu Ile Leu Thr Leu Tyr
385 390 395 400
ggt ggt aag atc tcc gag att tcc aac tct gaa att cat ttc cca cac 1248
Gly Gly Lys Ile Ser Glu Ile Ser Asn Ser Glu Ile His Phe Pro His
405 410 415
agg tct ggt aac ttg ttc aaa att cag tac atg gtt acc tgg gac gac 1296
Arg Ser Gly Asn Leu Phe Lys Ile Gln Tyr Met Val Thr Trp Asp Asp
420 425 430
tac aga gaa aac gaa att ggt gaa tcc ctg atg aga aag ttg tac gat 1344
Tyr Arg Glu Asn Glu Ile Gly Glu Ser Leu Met Arg Lys Leu Tyr Asp
435 440 445
tat atg gct ccc tac gtt tct aaa tct cca aga gct gct tat ctg aac 1392
Tyr Met Ala Pro Tyr Val Ser Lys Ser Pro Arg Ala Ala Tyr Leu Asn
450 455 460
tac aag gat ttg gac tta ggt aga aac aac gat gtt cat act tct tac 1440
Tyr Lys Asp Leu Asp Leu Gly Arg Asn Asn Asp Val His Thr Ser Tyr
465 470 475 480
gct caa gct agt att tgg ggc ttg aag tac ttt aag aac aac ttc aga 1488
Ala Gln Ala Ser Ile Trp Gly Leu Lys Tyr Phe Lys Asn Asn Phe Arg
485 490 495
aga ttg gtc cac gtt aag act ttg gtt gat cca ggt aat ttc ttc agg 1536
Arg Leu Val His Val Lys Thr Leu Val Asp Pro Gly Asn Phe Phe Arg
500 505 510
gac gaa caa tct att cca tcc gtt aga tct tcc cgt aag aag tgg tca 1584
Asp Glu Gln Ser Ile Pro Ser Val Arg Ser Ser Arg Lys Lys Trp Ser
515 520 525
cat cca caa ttt gaa aag 1602
His Pro Gln Phe Glu Lys
530
<210> 34
<211> 534
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 34
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser
1 5 10 15
Ala Leu Ala Met Gln Ile Ile Ile Thr Lys Arg Ser Ser Ala Tyr Phe
20 25 30
Ser Ala Val Gln Ser Ser Ile Gln Asn Leu Ile Phe Val Thr Arg Gly
35 40 45
Thr Ser Arg Pro Glu Ala Ile Ile Thr Leu Leu His Asp Ser His Gly
50 55 60
Val Leu Ile Arg Ile Arg Ser Arg Gly His Asp Tyr Glu Gly Gln Ser
65 70 75 80
Tyr Val Ala Arg Ala Pro Phe Val Ile Ile Asp Leu Phe Asn Leu Arg
85 90 95
Ser Ile Asp Val Asp Ile Glu Asn Gly Ser Ala Trp Val Glu Ser Gly
100 105 110
Ala Thr Leu Gly Glu Leu Tyr Tyr Arg Ile Ala Glu Lys Ser Lys Ala
115 120 125
Tyr Gly Phe Pro Ala Gly Ser Cys Pro Thr Ile Gly Val Gly Gly His
130 135 140
Ile Ser Gly Gly Gly Phe Gly Thr Leu Phe Arg Lys Tyr Gly Leu Ala
145 150 155 160
Ala Asp Asn Val Leu Asp Ala Lys Ile Val Asp Val Asn Gly Arg Val
165 170 175
Leu Asp Arg Lys Pro Met Gly Glu Glu Leu Phe Trp Ala Ile Arg Gly
180 185 190
Gly Gly Gly Ser Ser Phe Gly Val Ile Leu Ala Trp Lys Leu Lys Leu
195 200 205
Val Pro Val Pro Pro Ser Val Thr Arg His Glu Asp Leu Phe Leu His
210 215 220
Ser Val Ile Gly Val Gly Asn Lys Ala Gly Thr Asn Gly Asp Lys Thr
225 230 235 240
Ile Ile Ile Glu Phe Ser Ser Leu Phe Leu Gly Pro Val Glu Lys Leu
245 250 255
Leu Pro Ser Met Gln Asp Asn Phe Ser Glu Leu Ser Val Asp Arg Ser
260 265 270
Asp Cys Thr Glu Met Ser Trp Ile Glu Ser Val Leu Tyr Phe Ala Phe
275 280 285
Ile Pro Arg Asn Glu Ser Glu Ala Leu Leu Gly Arg Thr Gln Gln Ser
290 295 300
Lys Ser Phe Ala Lys Pro Gln Gly Ile Phe Trp Thr His Arg Thr Pro
305 310 315 320
Thr Met Asn Val Asn Ser Pro Thr Ser Lys Phe Cys Thr Thr Cys Thr
325 330 335
Pro Ser Lys Thr Tyr Lys Arg Gly Thr Ser Pro Lys Ile Glu Val Thr
340 345 350
Gly Thr Phe Gly Thr Leu Lys Thr Ser Thr Ser Val Phe Lys Ala Lys
355 360 365
Ser Asp Tyr Val Asn Glu Pro Ile Ser Lys Ala Gly Leu Glu Gly Leu
370 375 380
Trp Gln Thr Leu Ile Glu Val Lys Ala Tyr Leu Ile Leu Thr Leu Tyr
385 390 395 400
Gly Gly Lys Ile Ser Glu Ile Ser Asn Ser Glu Ile His Phe Pro His
405 410 415
Arg Ser Gly Asn Leu Phe Lys Ile Gln Tyr Met Val Thr Trp Asp Asp
420 425 430
Tyr Arg Glu Asn Glu Ile Gly Glu Ser Leu Met Arg Lys Leu Tyr Asp
435 440 445
Tyr Met Ala Pro Tyr Val Ser Lys Ser Pro Arg Ala Ala Tyr Leu Asn
450 455 460
Tyr Lys Asp Leu Asp Leu Gly Arg Asn Asn Asp Val His Thr Ser Tyr
465 470 475 480
Ala Gln Ala Ser Ile Trp Gly Leu Lys Tyr Phe Lys Asn Asn Phe Arg
485 490 495
Arg Leu Val His Val Lys Thr Leu Val Asp Pro Gly Asn Phe Phe Arg
500 505 510
Asp Glu Gln Ser Ile Pro Ser Val Arg Ser Ser Arg Lys Lys Trp Ser
515 520 525
His Pro Gln Phe Glu Lys
530
<210> 35
<211> 1812
<212> DNA
<213> 人工序列
<220>
<223> CBS35-M5X864-全长alpha因子-strep标签
<400> 35
atgagatttc cttcaatttt tactgctgtt ttattcgcag catcctccgc attagctgct 60
ccagtcaaca ctacaacaga agatgaaacg gcacaaattc cggctgaagc tgtcatcggt 120
tactcagatt tagaagggga tttcgatgtt gctgttttgc cattttccaa cagcacaaat 180
aacgggttat tgtttataaa tactactatt gccagcattg ctgctaaaga agaaggggta 240
tctctcgaga aaagagaggc tgaagctatg cagatcatta tcaccaagag atcctctgct 300
tacttctctg ctgttcaatc ctctatccaa aacttgatct tcgttaccag aggtacttct 360
agaccagaag ctattattac cttgttgcat gattctcacg gtgtcttgat tagaatcaga 420
tctagaggtc atgactacga aggtcaatct tatgttgcta gagccccatt cgttatcatc 480
gatttgttta acttgaggtc catcgacgtt gacattgaaa atggttctgc ttgggttgaa 540
tctggtgcta ctttgggtga gttgtattac agaattgccg aaaagtctaa ggcttacggt 600
tttccagctg gttcttgtcc aactattggt gttggtggtc atatttctgg tggtggtttt 660
ggtactttgt tcagaaagta tggtttggct gctgataacg ttttggatgc taaaatcgtt 720
gatgtcaacg gtagagtttt ggacagaaaa cctatgggtg aagaattatt ctgggctatt 780
agaggtggtg gtggatcttc ttttggtgtt attttggctt ggaagttgaa gttggttcca 840
gttccaccat ctgttactag acatgaagat ttgttcttgc actccgttat aggtgttggt 900
aacaaagctg gtactaatgg tgataagacc attattatcg agttcagctc cttgtttttg 960
ggtccagttg aaaagttgtt gccatccatg caagataact tctccgaatt gtctgttgat 1020
agatccgatt gcactgaaat gtcctggatt gaatctgtct tgtacttcgc tttcatccca 1080
agaaatgaat ctgaagcctt gttgggtaga acccaacaat ctaaatcttt cgctaagcca 1140
caaggtattt tctggactca tagaactcca accatgaacg ttaattctcc aacctctaag 1200
ttctgtacta cttgtactcc atctaagacc tacaagagag gtacatctcc aaagattgaa 1260
gttactggta ctttcggcac tttgaaaact tctacctctg ttttcaaggc caagtccgat 1320
tatgttaacg aacctatttc taaggctggt ttggaaggtt tgtggcaaac tttgatagaa 1380
gttaaggcct acctgatctt gacattatac ggtggtaaga tctccgagat ttccaactct 1440
gaaattcatt tcccacacag gtctggtaac ttgttcaaaa ttcagtacat ggttacctgg 1500
gacgactaca gagaaaacga aattggtgaa tccctgatga gaaagttgta cgattatatg 1560
gctccctacg tttctaaatc tccaagagct gcttatctga actacaagga tttggactta 1620
ggtagaaaca acgatgttca tacttcttac gctcaagcta gtatttgggg cttgaagtac 1680
tttaagaaca acttcagaag attggtccac gttaagactt tggttgatcc aggtaatttc 1740
ttcagggacg aacaatctat tccatccgtt agatcttccc gtaagaagtg gtcacatcca 1800
caatttgaaa ag 1812
<210> 36
<211> 1620
<212> DNA
<213> 人工序列
<220>
<223> CBS59-O64745
<220>
<221> CDS
<222> (1)..(1620)
<400> 36
atg aag ttc tgg tca aga cct ttg acc ttc ttg atc atc atc atc tac 48
Met Lys Phe Trp Ser Arg Pro Leu Thr Phe Leu Ile Ile Ile Ile Tyr
1 5 10 15
ctg atc atc caa cag gtt aat tct tct cca cca tcc ttg tct att cca 96
Leu Ile Ile Gln Gln Val Asn Ser Ser Pro Pro Ser Leu Ser Ile Pro
20 25 30
gaa cat ttc ttg aga tgc ttg gat acc caa cca tct gat cat ggt tct 144
Glu His Phe Leu Arg Cys Leu Asp Thr Gln Pro Ser Asp His Gly Ser
35 40 45
cca aat tct aga act gcc gtt att cca acg aac tct tct ttt tct acc 192
Pro Asn Ser Arg Thr Ala Val Ile Pro Thr Asn Ser Ser Phe Ser Thr
50 55 60
aac ttg atg aac ggc gtc aga aat ttg aga ttc gct tct gtt tct act 240
Asn Leu Met Asn Gly Val Arg Asn Leu Arg Phe Ala Ser Val Ser Thr
65 70 75 80
agg aag cca gaa gtt ata gtt gct gct gtt act gaa acc cat att aga 288
Arg Lys Pro Glu Val Ile Val Ala Ala Val Thr Glu Thr His Ile Arg
85 90 95
gct acc att tct tgc tgc aag ttg ttg aac ttg gaa ttg aga att aga 336
Ala Thr Ile Ser Cys Cys Lys Leu Leu Asn Leu Glu Leu Arg Ile Arg
100 105 110
tcc ggt ggt cat gat tac gaa ggt ttc tct tat act tct cca gtt cca 384
Ser Gly Gly His Asp Tyr Glu Gly Phe Ser Tyr Thr Ser Pro Val Pro
115 120 125
ttc gtt atc ctg gac atg tac aac ttc aac aag atc gac att aac atg 432
Phe Val Ile Leu Asp Met Tyr Asn Phe Asn Lys Ile Asp Ile Asn Met
130 135 140
aag gac gaa acc gtc tgg att caa tct ggt gct tct ttg ggt cag ttg 480
Lys Asp Glu Thr Val Trp Ile Gln Ser Gly Ala Ser Leu Gly Gln Leu
145 150 155 160
tac tac aat att gcc tcc aag tct aag gtt cat gct ttt cca gct ggt 528
Tyr Tyr Asn Ile Ala Ser Lys Ser Lys Val His Ala Phe Pro Ala Gly
165 170 175
gtt tgt cca aaa gtt ggt gct ggt ggt cat ttt tct ggt ggt ggt ttt 576
Val Cys Pro Lys Val Gly Ala Gly Gly His Phe Ser Gly Gly Gly Phe
180 185 190
ggt aat ttg atg agg aaa tac ggt ttg tcc atc gat cat atc att gac 624
Gly Asn Leu Met Arg Lys Tyr Gly Leu Ser Ile Asp His Ile Ile Asp
195 200 205
gcc caa att atg gat gct aac ggt aag gtt tac aga aac aga caa gct 672
Ala Gln Ile Met Asp Ala Asn Gly Lys Val Tyr Arg Asn Arg Gln Ala
210 215 220
atg ggt gaa gat gtt ttc tgg gct att aga ggt ggt ggt ggc ggt tct 720
Met Gly Glu Asp Val Phe Trp Ala Ile Arg Gly Gly Gly Gly Gly Ser
225 230 235 240
tat ggt gtt att ttg gct tgg aag att aag ttg gtt agg gtt cca gaa 768
Tyr Gly Val Ile Leu Ala Trp Lys Ile Lys Leu Val Arg Val Pro Glu
245 250 255
aag gtc acc gtt ttt aag ttg gaa aga acc gtt aga gaa ggt gcc gtt 816
Lys Val Thr Val Phe Lys Leu Glu Arg Thr Val Arg Glu Gly Ala Val
260 265 270
gat ttg gtt cat aag tgg caa caa gtt gcc cca gtt att gat aga gat 864
Asp Leu Val His Lys Trp Gln Gln Val Ala Pro Val Ile Asp Arg Asp
275 280 285
ttg ttc atc aga ttg gag atc aag ccc atc aac aga aag att tct aag 912
Leu Phe Ile Arg Leu Glu Ile Lys Pro Ile Asn Arg Lys Ile Ser Lys
290 295 300
ggt aag acc atc aag gtg tcc ttc att ggt atg ttt ttg ggt ttg cca 960
Gly Lys Thr Ile Lys Val Ser Phe Ile Gly Met Phe Leu Gly Leu Pro
305 310 315 320
gaa cgt ttg ttg aac atc act aag caa tct ttc cca gaa ctg cac ttg 1008
Glu Arg Leu Leu Asn Ile Thr Lys Gln Ser Phe Pro Glu Leu His Leu
325 330 335
aca aaa gaa gat tgc atg gtt aag aag tgg atc gag tca tct gtt ttt 1056
Thr Lys Glu Asp Cys Met Val Lys Lys Trp Ile Glu Ser Ser Val Phe
340 345 350
tgg gca aat tac cca gaa aaa gcc cca att gag ttg ttg ttg aaa aga 1104
Trp Ala Asn Tyr Pro Glu Lys Ala Pro Ile Glu Leu Leu Leu Lys Arg
355 360 365
gtc tct acc aac gag tac tac tgg aag aga act tct gat ttt gtt cag 1152
Val Ser Thr Asn Glu Tyr Tyr Trp Lys Arg Thr Ser Asp Phe Val Gln
370 375 380
gcc cca att tct aaa caa ggt ttg gct aag att ttc cag acc atg att 1200
Ala Pro Ile Ser Lys Gln Gly Leu Ala Lys Ile Phe Gln Thr Met Ile
385 390 395 400
gat cat tct cca ttg cct aga aga gtt tgg atg caa tgg aat cct tgg 1248
Asp His Ser Pro Leu Pro Arg Arg Val Trp Met Gln Trp Asn Pro Trp
405 410 415
ggt ggt aaa atg gct gaa att gct tct gat gct act gcc ttt gtt cac 1296
Gly Gly Lys Met Ala Glu Ile Ala Ser Asp Ala Thr Ala Phe Val His
420 425 430
aga ggt ggt aat gtt ttc atg atc gaa cat ttc atg aac tgg tac aga 1344
Arg Gly Gly Asn Val Phe Met Ile Glu His Phe Met Asn Trp Tyr Arg
435 440 445
cca ggt gat gaa ttg gaa gaa aag ttc ttg gct att gcc agg tct ttc 1392
Pro Gly Asp Glu Leu Glu Glu Lys Phe Leu Ala Ile Ala Arg Ser Phe
450 455 460
aaa gaa gct atg gct cca ttt gtt tct aag aat cca aga gaa gcc ttc 1440
Lys Glu Ala Met Ala Pro Phe Val Ser Lys Asn Pro Arg Glu Ala Phe
465 470 475 480
ttc aac tac aga gat gtt gat att ggt atc act acc cca ggt tac aat 1488
Phe Asn Tyr Arg Asp Val Asp Ile Gly Ile Thr Thr Pro Gly Tyr Asn
485 490 495
gct act tat gaa ggt gct aaa gtt tac ggc gat tct tac ttt aag ggt 1536
Ala Thr Tyr Glu Gly Ala Lys Val Tyr Gly Asp Ser Tyr Phe Lys Gly
500 505 510
aac tac ttg agg ttg gtt aag atc aag gct aga ttc gac aga acg aac 1584
Asn Tyr Leu Arg Leu Val Lys Ile Lys Ala Arg Phe Asp Arg Thr Asn
515 520 525
ttc ttc aga tct caa caa ggt att cca gtt ttg gcc 1620
Phe Phe Arg Ser Gln Gln Gly Ile Pro Val Leu Ala
530 535 540
<210> 37
<211> 540
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 37
Met Lys Phe Trp Ser Arg Pro Leu Thr Phe Leu Ile Ile Ile Ile Tyr
1 5 10 15
Leu Ile Ile Gln Gln Val Asn Ser Ser Pro Pro Ser Leu Ser Ile Pro
20 25 30
Glu His Phe Leu Arg Cys Leu Asp Thr Gln Pro Ser Asp His Gly Ser
35 40 45
Pro Asn Ser Arg Thr Ala Val Ile Pro Thr Asn Ser Ser Phe Ser Thr
50 55 60
Asn Leu Met Asn Gly Val Arg Asn Leu Arg Phe Ala Ser Val Ser Thr
65 70 75 80
Arg Lys Pro Glu Val Ile Val Ala Ala Val Thr Glu Thr His Ile Arg
85 90 95
Ala Thr Ile Ser Cys Cys Lys Leu Leu Asn Leu Glu Leu Arg Ile Arg
100 105 110
Ser Gly Gly His Asp Tyr Glu Gly Phe Ser Tyr Thr Ser Pro Val Pro
115 120 125
Phe Val Ile Leu Asp Met Tyr Asn Phe Asn Lys Ile Asp Ile Asn Met
130 135 140
Lys Asp Glu Thr Val Trp Ile Gln Ser Gly Ala Ser Leu Gly Gln Leu
145 150 155 160
Tyr Tyr Asn Ile Ala Ser Lys Ser Lys Val His Ala Phe Pro Ala Gly
165 170 175
Val Cys Pro Lys Val Gly Ala Gly Gly His Phe Ser Gly Gly Gly Phe
180 185 190
Gly Asn Leu Met Arg Lys Tyr Gly Leu Ser Ile Asp His Ile Ile Asp
195 200 205
Ala Gln Ile Met Asp Ala Asn Gly Lys Val Tyr Arg Asn Arg Gln Ala
210 215 220
Met Gly Glu Asp Val Phe Trp Ala Ile Arg Gly Gly Gly Gly Gly Ser
225 230 235 240
Tyr Gly Val Ile Leu Ala Trp Lys Ile Lys Leu Val Arg Val Pro Glu
245 250 255
Lys Val Thr Val Phe Lys Leu Glu Arg Thr Val Arg Glu Gly Ala Val
260 265 270
Asp Leu Val His Lys Trp Gln Gln Val Ala Pro Val Ile Asp Arg Asp
275 280 285
Leu Phe Ile Arg Leu Glu Ile Lys Pro Ile Asn Arg Lys Ile Ser Lys
290 295 300
Gly Lys Thr Ile Lys Val Ser Phe Ile Gly Met Phe Leu Gly Leu Pro
305 310 315 320
Glu Arg Leu Leu Asn Ile Thr Lys Gln Ser Phe Pro Glu Leu His Leu
325 330 335
Thr Lys Glu Asp Cys Met Val Lys Lys Trp Ile Glu Ser Ser Val Phe
340 345 350
Trp Ala Asn Tyr Pro Glu Lys Ala Pro Ile Glu Leu Leu Leu Lys Arg
355 360 365
Val Ser Thr Asn Glu Tyr Tyr Trp Lys Arg Thr Ser Asp Phe Val Gln
370 375 380
Ala Pro Ile Ser Lys Gln Gly Leu Ala Lys Ile Phe Gln Thr Met Ile
385 390 395 400
Asp His Ser Pro Leu Pro Arg Arg Val Trp Met Gln Trp Asn Pro Trp
405 410 415
Gly Gly Lys Met Ala Glu Ile Ala Ser Asp Ala Thr Ala Phe Val His
420 425 430
Arg Gly Gly Asn Val Phe Met Ile Glu His Phe Met Asn Trp Tyr Arg
435 440 445
Pro Gly Asp Glu Leu Glu Glu Lys Phe Leu Ala Ile Ala Arg Ser Phe
450 455 460
Lys Glu Ala Met Ala Pro Phe Val Ser Lys Asn Pro Arg Glu Ala Phe
465 470 475 480
Phe Asn Tyr Arg Asp Val Asp Ile Gly Ile Thr Thr Pro Gly Tyr Asn
485 490 495
Ala Thr Tyr Glu Gly Ala Lys Val Tyr Gly Asp Ser Tyr Phe Lys Gly
500 505 510
Asn Tyr Leu Arg Leu Val Lys Ile Lys Ala Arg Phe Asp Arg Thr Asn
515 520 525
Phe Phe Arg Ser Gln Gln Gly Ile Pro Val Leu Ala
530 535 540
<210> 38
<211> 1551
<212> DNA
<213> 人工序列
<220>
<223> CBS59-O64745-无信号肽
<220>
<221> CDS
<222> (1)..(1551)
<400> 38
atg tct cca cca tcc ttg tct att cca gaa cat ttc ttg aga tgc ttg 48
Met Ser Pro Pro Ser Leu Ser Ile Pro Glu His Phe Leu Arg Cys Leu
1 5 10 15
gat acc caa cca tct gat cat ggt tct cca aat tct aga act gcc gtt 96
Asp Thr Gln Pro Ser Asp His Gly Ser Pro Asn Ser Arg Thr Ala Val
20 25 30
att cca acg aac tct tct ttt tct acc aac ttg atg aac ggc gtc aga 144
Ile Pro Thr Asn Ser Ser Phe Ser Thr Asn Leu Met Asn Gly Val Arg
35 40 45
aat ttg aga ttc gct tct gtt tct act agg aag cca gaa gtt ata gtt 192
Asn Leu Arg Phe Ala Ser Val Ser Thr Arg Lys Pro Glu Val Ile Val
50 55 60
gct gct gtt act gaa acc cat att aga gct acc att tct tgc tgc aag 240
Ala Ala Val Thr Glu Thr His Ile Arg Ala Thr Ile Ser Cys Cys Lys
65 70 75 80
ttg ttg aac ttg gaa ttg aga att aga tcc ggt ggt cat gat tac gaa 288
Leu Leu Asn Leu Glu Leu Arg Ile Arg Ser Gly Gly His Asp Tyr Glu
85 90 95
ggt ttc tct tat act tct cca gtt cca ttc gtt atc ctg gac atg tac 336
Gly Phe Ser Tyr Thr Ser Pro Val Pro Phe Val Ile Leu Asp Met Tyr
100 105 110
aac ttc aac aag atc gac att aac atg aag gac gaa acc gtc tgg att 384
Asn Phe Asn Lys Ile Asp Ile Asn Met Lys Asp Glu Thr Val Trp Ile
115 120 125
caa tct ggt gct tct ttg ggt cag ttg tac tac aat att gcc tcc aag 432
Gln Ser Gly Ala Ser Leu Gly Gln Leu Tyr Tyr Asn Ile Ala Ser Lys
130 135 140
tct aag gtt cat gct ttt cca gct ggt gtt tgt cca aaa gtt ggt gct 480
Ser Lys Val His Ala Phe Pro Ala Gly Val Cys Pro Lys Val Gly Ala
145 150 155 160
ggt ggt cat ttt tct ggt ggt ggt ttt ggt aat ttg atg agg aaa tac 528
Gly Gly His Phe Ser Gly Gly Gly Phe Gly Asn Leu Met Arg Lys Tyr
165 170 175
ggt ttg tcc atc gat cat atc att gac gcc caa att atg gat gct aac 576
Gly Leu Ser Ile Asp His Ile Ile Asp Ala Gln Ile Met Asp Ala Asn
180 185 190
ggt aag gtt tac aga aac aga caa gct atg ggt gaa gat gtt ttc tgg 624
Gly Lys Val Tyr Arg Asn Arg Gln Ala Met Gly Glu Asp Val Phe Trp
195 200 205
gct att aga ggt ggt ggt ggc ggt tct tat ggt gtt att ttg gct tgg 672
Ala Ile Arg Gly Gly Gly Gly Gly Ser Tyr Gly Val Ile Leu Ala Trp
210 215 220
aag att aag ttg gtt agg gtt cca gaa aag gtc acc gtt ttt aag ttg 720
Lys Ile Lys Leu Val Arg Val Pro Glu Lys Val Thr Val Phe Lys Leu
225 230 235 240
gaa aga acc gtt aga gaa ggt gcc gtt gat ttg gtt cat aag tgg caa 768
Glu Arg Thr Val Arg Glu Gly Ala Val Asp Leu Val His Lys Trp Gln
245 250 255
caa gtt gcc cca gtt att gat aga gat ttg ttc atc aga ttg gag atc 816
Gln Val Ala Pro Val Ile Asp Arg Asp Leu Phe Ile Arg Leu Glu Ile
260 265 270
aag ccc atc aac aga aag att tct aag ggt aag acc atc aag gtg tcc 864
Lys Pro Ile Asn Arg Lys Ile Ser Lys Gly Lys Thr Ile Lys Val Ser
275 280 285
ttc att ggt atg ttt ttg ggt ttg cca gaa cgt ttg ttg aac atc act 912
Phe Ile Gly Met Phe Leu Gly Leu Pro Glu Arg Leu Leu Asn Ile Thr
290 295 300
aag caa tct ttc cca gaa ctg cac ttg aca aaa gaa gat tgc atg gtt 960
Lys Gln Ser Phe Pro Glu Leu His Leu Thr Lys Glu Asp Cys Met Val
305 310 315 320
aag aag tgg atc gag tca tct gtt ttt tgg gca aat tac cca gaa aaa 1008
Lys Lys Trp Ile Glu Ser Ser Val Phe Trp Ala Asn Tyr Pro Glu Lys
325 330 335
gcc cca att gag ttg ttg ttg aaa aga gtc tct acc aac gag tac tac 1056
Ala Pro Ile Glu Leu Leu Leu Lys Arg Val Ser Thr Asn Glu Tyr Tyr
340 345 350
tgg aag aga act tct gat ttt gtt cag gcc cca att tct aaa caa ggt 1104
Trp Lys Arg Thr Ser Asp Phe Val Gln Ala Pro Ile Ser Lys Gln Gly
355 360 365
ttg gct aag att ttc cag acc atg att gat cat tct cca ttg cct aga 1152
Leu Ala Lys Ile Phe Gln Thr Met Ile Asp His Ser Pro Leu Pro Arg
370 375 380
aga gtt tgg atg caa tgg aat cct tgg ggt ggt aaa atg gct gaa att 1200
Arg Val Trp Met Gln Trp Asn Pro Trp Gly Gly Lys Met Ala Glu Ile
385 390 395 400
gct tct gat gct act gcc ttt gtt cac aga ggt ggt aat gtt ttc atg 1248
Ala Ser Asp Ala Thr Ala Phe Val His Arg Gly Gly Asn Val Phe Met
405 410 415
atc gaa cat ttc atg aac tgg tac aga cca ggt gat gaa ttg gaa gaa 1296
Ile Glu His Phe Met Asn Trp Tyr Arg Pro Gly Asp Glu Leu Glu Glu
420 425 430
aag ttc ttg gct att gcc agg tct ttc aaa gaa gct atg gct cca ttt 1344
Lys Phe Leu Ala Ile Ala Arg Ser Phe Lys Glu Ala Met Ala Pro Phe
435 440 445
gtt tct aag aat cca aga gaa gcc ttc ttc aac tac aga gat gtt gat 1392
Val Ser Lys Asn Pro Arg Glu Ala Phe Phe Asn Tyr Arg Asp Val Asp
450 455 460
att ggt atc act acc cca ggt tac aat gct act tat gaa ggt gct aaa 1440
Ile Gly Ile Thr Thr Pro Gly Tyr Asn Ala Thr Tyr Glu Gly Ala Lys
465 470 475 480
gtt tac ggc gat tct tac ttt aag ggt aac tac ttg agg ttg gtt aag 1488
Val Tyr Gly Asp Ser Tyr Phe Lys Gly Asn Tyr Leu Arg Leu Val Lys
485 490 495
atc aag gct aga ttc gac aga acg aac ttc ttc aga tct caa caa ggt 1536
Ile Lys Ala Arg Phe Asp Arg Thr Asn Phe Phe Arg Ser Gln Gln Gly
500 505 510
att cca gtt ttg gcc 1551
Ile Pro Val Leu Ala
515
<210> 39
<211> 517
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 39
Met Ser Pro Pro Ser Leu Ser Ile Pro Glu His Phe Leu Arg Cys Leu
1 5 10 15
Asp Thr Gln Pro Ser Asp His Gly Ser Pro Asn Ser Arg Thr Ala Val
20 25 30
Ile Pro Thr Asn Ser Ser Phe Ser Thr Asn Leu Met Asn Gly Val Arg
35 40 45
Asn Leu Arg Phe Ala Ser Val Ser Thr Arg Lys Pro Glu Val Ile Val
50 55 60
Ala Ala Val Thr Glu Thr His Ile Arg Ala Thr Ile Ser Cys Cys Lys
65 70 75 80
Leu Leu Asn Leu Glu Leu Arg Ile Arg Ser Gly Gly His Asp Tyr Glu
85 90 95
Gly Phe Ser Tyr Thr Ser Pro Val Pro Phe Val Ile Leu Asp Met Tyr
100 105 110
Asn Phe Asn Lys Ile Asp Ile Asn Met Lys Asp Glu Thr Val Trp Ile
115 120 125
Gln Ser Gly Ala Ser Leu Gly Gln Leu Tyr Tyr Asn Ile Ala Ser Lys
130 135 140
Ser Lys Val His Ala Phe Pro Ala Gly Val Cys Pro Lys Val Gly Ala
145 150 155 160
Gly Gly His Phe Ser Gly Gly Gly Phe Gly Asn Leu Met Arg Lys Tyr
165 170 175
Gly Leu Ser Ile Asp His Ile Ile Asp Ala Gln Ile Met Asp Ala Asn
180 185 190
Gly Lys Val Tyr Arg Asn Arg Gln Ala Met Gly Glu Asp Val Phe Trp
195 200 205
Ala Ile Arg Gly Gly Gly Gly Gly Ser Tyr Gly Val Ile Leu Ala Trp
210 215 220
Lys Ile Lys Leu Val Arg Val Pro Glu Lys Val Thr Val Phe Lys Leu
225 230 235 240
Glu Arg Thr Val Arg Glu Gly Ala Val Asp Leu Val His Lys Trp Gln
245 250 255
Gln Val Ala Pro Val Ile Asp Arg Asp Leu Phe Ile Arg Leu Glu Ile
260 265 270
Lys Pro Ile Asn Arg Lys Ile Ser Lys Gly Lys Thr Ile Lys Val Ser
275 280 285
Phe Ile Gly Met Phe Leu Gly Leu Pro Glu Arg Leu Leu Asn Ile Thr
290 295 300
Lys Gln Ser Phe Pro Glu Leu His Leu Thr Lys Glu Asp Cys Met Val
305 310 315 320
Lys Lys Trp Ile Glu Ser Ser Val Phe Trp Ala Asn Tyr Pro Glu Lys
325 330 335
Ala Pro Ile Glu Leu Leu Leu Lys Arg Val Ser Thr Asn Glu Tyr Tyr
340 345 350
Trp Lys Arg Thr Ser Asp Phe Val Gln Ala Pro Ile Ser Lys Gln Gly
355 360 365
Leu Ala Lys Ile Phe Gln Thr Met Ile Asp His Ser Pro Leu Pro Arg
370 375 380
Arg Val Trp Met Gln Trp Asn Pro Trp Gly Gly Lys Met Ala Glu Ile
385 390 395 400
Ala Ser Asp Ala Thr Ala Phe Val His Arg Gly Gly Asn Val Phe Met
405 410 415
Ile Glu His Phe Met Asn Trp Tyr Arg Pro Gly Asp Glu Leu Glu Glu
420 425 430
Lys Phe Leu Ala Ile Ala Arg Ser Phe Lys Glu Ala Met Ala Pro Phe
435 440 445
Val Ser Lys Asn Pro Arg Glu Ala Phe Phe Asn Tyr Arg Asp Val Asp
450 455 460
Ile Gly Ile Thr Thr Pro Gly Tyr Asn Ala Thr Tyr Glu Gly Ala Lys
465 470 475 480
Val Tyr Gly Asp Ser Tyr Phe Lys Gly Asn Tyr Leu Arg Leu Val Lys
485 490 495
Ile Lys Ala Arg Phe Asp Arg Thr Asn Phe Phe Arg Ser Gln Gln Gly
500 505 510
Ile Pro Val Leu Ala
515
<210> 40
<211> 1575
<212> DNA
<213> 人工序列
<220>
<223> CBS59-O64745-无信号肽-strep标签
<220>
<221> CDS
<222> (1)..(1575)
<400> 40
atg tct cca cca tcc ttg tct att cca gaa cat ttc ttg aga tgc ttg 48
Met Ser Pro Pro Ser Leu Ser Ile Pro Glu His Phe Leu Arg Cys Leu
1 5 10 15
gat acc caa cca tct gat cat ggt tct cca aat tct aga act gcc gtt 96
Asp Thr Gln Pro Ser Asp His Gly Ser Pro Asn Ser Arg Thr Ala Val
20 25 30
att cca acg aac tct tct ttt tct acc aac ttg atg aac ggc gtc aga 144
Ile Pro Thr Asn Ser Ser Phe Ser Thr Asn Leu Met Asn Gly Val Arg
35 40 45
aat ttg aga ttc gct tct gtt tct act agg aag cca gaa gtt ata gtt 192
Asn Leu Arg Phe Ala Ser Val Ser Thr Arg Lys Pro Glu Val Ile Val
50 55 60
gct gct gtt act gaa acc cat att aga gct acc att tct tgc tgc aag 240
Ala Ala Val Thr Glu Thr His Ile Arg Ala Thr Ile Ser Cys Cys Lys
65 70 75 80
ttg ttg aac ttg gaa ttg aga att aga tcc ggt ggt cat gat tac gaa 288
Leu Leu Asn Leu Glu Leu Arg Ile Arg Ser Gly Gly His Asp Tyr Glu
85 90 95
ggt ttc tct tat act tct cca gtt cca ttc gtt atc ctg gac atg tac 336
Gly Phe Ser Tyr Thr Ser Pro Val Pro Phe Val Ile Leu Asp Met Tyr
100 105 110
aac ttc aac aag atc gac att aac atg aag gac gaa acc gtc tgg att 384
Asn Phe Asn Lys Ile Asp Ile Asn Met Lys Asp Glu Thr Val Trp Ile
115 120 125
caa tct ggt gct tct ttg ggt cag ttg tac tac aat att gcc tcc aag 432
Gln Ser Gly Ala Ser Leu Gly Gln Leu Tyr Tyr Asn Ile Ala Ser Lys
130 135 140
tct aag gtt cat gct ttt cca gct ggt gtt tgt cca aaa gtt ggt gct 480
Ser Lys Val His Ala Phe Pro Ala Gly Val Cys Pro Lys Val Gly Ala
145 150 155 160
ggt ggt cat ttt tct ggt ggt ggt ttt ggt aat ttg atg agg aaa tac 528
Gly Gly His Phe Ser Gly Gly Gly Phe Gly Asn Leu Met Arg Lys Tyr
165 170 175
ggt ttg tcc atc gat cat atc att gac gcc caa att atg gat gct aac 576
Gly Leu Ser Ile Asp His Ile Ile Asp Ala Gln Ile Met Asp Ala Asn
180 185 190
ggt aag gtt tac aga aac aga caa gct atg ggt gaa gat gtt ttc tgg 624
Gly Lys Val Tyr Arg Asn Arg Gln Ala Met Gly Glu Asp Val Phe Trp
195 200 205
gct att aga ggt ggt ggt ggc ggt tct tat ggt gtt att ttg gct tgg 672
Ala Ile Arg Gly Gly Gly Gly Gly Ser Tyr Gly Val Ile Leu Ala Trp
210 215 220
aag att aag ttg gtt agg gtt cca gaa aag gtc acc gtt ttt aag ttg 720
Lys Ile Lys Leu Val Arg Val Pro Glu Lys Val Thr Val Phe Lys Leu
225 230 235 240
gaa aga acc gtt aga gaa ggt gcc gtt gat ttg gtt cat aag tgg caa 768
Glu Arg Thr Val Arg Glu Gly Ala Val Asp Leu Val His Lys Trp Gln
245 250 255
caa gtt gcc cca gtt att gat aga gat ttg ttc atc aga ttg gag atc 816
Gln Val Ala Pro Val Ile Asp Arg Asp Leu Phe Ile Arg Leu Glu Ile
260 265 270
aag ccc atc aac aga aag att tct aag ggt aag acc atc aag gtg tcc 864
Lys Pro Ile Asn Arg Lys Ile Ser Lys Gly Lys Thr Ile Lys Val Ser
275 280 285
ttc att ggt atg ttt ttg ggt ttg cca gaa cgt ttg ttg aac atc act 912
Phe Ile Gly Met Phe Leu Gly Leu Pro Glu Arg Leu Leu Asn Ile Thr
290 295 300
aag caa tct ttc cca gaa ctg cac ttg aca aaa gaa gat tgc atg gtt 960
Lys Gln Ser Phe Pro Glu Leu His Leu Thr Lys Glu Asp Cys Met Val
305 310 315 320
aag aag tgg atc gag tca tct gtt ttt tgg gca aat tac cca gaa aaa 1008
Lys Lys Trp Ile Glu Ser Ser Val Phe Trp Ala Asn Tyr Pro Glu Lys
325 330 335
gcc cca att gag ttg ttg ttg aaa aga gtc tct acc aac gag tac tac 1056
Ala Pro Ile Glu Leu Leu Leu Lys Arg Val Ser Thr Asn Glu Tyr Tyr
340 345 350
tgg aag aga act tct gat ttt gtt cag gcc cca att tct aaa caa ggt 1104
Trp Lys Arg Thr Ser Asp Phe Val Gln Ala Pro Ile Ser Lys Gln Gly
355 360 365
ttg gct aag att ttc cag acc atg att gat cat tct cca ttg cct aga 1152
Leu Ala Lys Ile Phe Gln Thr Met Ile Asp His Ser Pro Leu Pro Arg
370 375 380
aga gtt tgg atg caa tgg aat cct tgg ggt ggt aaa atg gct gaa att 1200
Arg Val Trp Met Gln Trp Asn Pro Trp Gly Gly Lys Met Ala Glu Ile
385 390 395 400
gct tct gat gct act gcc ttt gtt cac aga ggt ggt aat gtt ttc atg 1248
Ala Ser Asp Ala Thr Ala Phe Val His Arg Gly Gly Asn Val Phe Met
405 410 415
atc gaa cat ttc atg aac tgg tac aga cca ggt gat gaa ttg gaa gaa 1296
Ile Glu His Phe Met Asn Trp Tyr Arg Pro Gly Asp Glu Leu Glu Glu
420 425 430
aag ttc ttg gct att gcc agg tct ttc aaa gaa gct atg gct cca ttt 1344
Lys Phe Leu Ala Ile Ala Arg Ser Phe Lys Glu Ala Met Ala Pro Phe
435 440 445
gtt tct aag aat cca aga gaa gcc ttc ttc aac tac aga gat gtt gat 1392
Val Ser Lys Asn Pro Arg Glu Ala Phe Phe Asn Tyr Arg Asp Val Asp
450 455 460
att ggt atc act acc cca ggt tac aat gct act tat gaa ggt gct aaa 1440
Ile Gly Ile Thr Thr Pro Gly Tyr Asn Ala Thr Tyr Glu Gly Ala Lys
465 470 475 480
gtt tac ggc gat tct tac ttt aag ggt aac tac ttg agg ttg gtt aag 1488
Val Tyr Gly Asp Ser Tyr Phe Lys Gly Asn Tyr Leu Arg Leu Val Lys
485 490 495
atc aag gct aga ttc gac aga acg aac ttc ttc aga tct caa caa ggt 1536
Ile Lys Ala Arg Phe Asp Arg Thr Asn Phe Phe Arg Ser Gln Gln Gly
500 505 510
att cca gtt ttg gcc tgg tca cat cca caa ttt gaa aag 1575
Ile Pro Val Leu Ala Trp Ser His Pro Gln Phe Glu Lys
515 520 525
<210> 41
<211> 525
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 41
Met Ser Pro Pro Ser Leu Ser Ile Pro Glu His Phe Leu Arg Cys Leu
1 5 10 15
Asp Thr Gln Pro Ser Asp His Gly Ser Pro Asn Ser Arg Thr Ala Val
20 25 30
Ile Pro Thr Asn Ser Ser Phe Ser Thr Asn Leu Met Asn Gly Val Arg
35 40 45
Asn Leu Arg Phe Ala Ser Val Ser Thr Arg Lys Pro Glu Val Ile Val
50 55 60
Ala Ala Val Thr Glu Thr His Ile Arg Ala Thr Ile Ser Cys Cys Lys
65 70 75 80
Leu Leu Asn Leu Glu Leu Arg Ile Arg Ser Gly Gly His Asp Tyr Glu
85 90 95
Gly Phe Ser Tyr Thr Ser Pro Val Pro Phe Val Ile Leu Asp Met Tyr
100 105 110
Asn Phe Asn Lys Ile Asp Ile Asn Met Lys Asp Glu Thr Val Trp Ile
115 120 125
Gln Ser Gly Ala Ser Leu Gly Gln Leu Tyr Tyr Asn Ile Ala Ser Lys
130 135 140
Ser Lys Val His Ala Phe Pro Ala Gly Val Cys Pro Lys Val Gly Ala
145 150 155 160
Gly Gly His Phe Ser Gly Gly Gly Phe Gly Asn Leu Met Arg Lys Tyr
165 170 175
Gly Leu Ser Ile Asp His Ile Ile Asp Ala Gln Ile Met Asp Ala Asn
180 185 190
Gly Lys Val Tyr Arg Asn Arg Gln Ala Met Gly Glu Asp Val Phe Trp
195 200 205
Ala Ile Arg Gly Gly Gly Gly Gly Ser Tyr Gly Val Ile Leu Ala Trp
210 215 220
Lys Ile Lys Leu Val Arg Val Pro Glu Lys Val Thr Val Phe Lys Leu
225 230 235 240
Glu Arg Thr Val Arg Glu Gly Ala Val Asp Leu Val His Lys Trp Gln
245 250 255
Gln Val Ala Pro Val Ile Asp Arg Asp Leu Phe Ile Arg Leu Glu Ile
260 265 270
Lys Pro Ile Asn Arg Lys Ile Ser Lys Gly Lys Thr Ile Lys Val Ser
275 280 285
Phe Ile Gly Met Phe Leu Gly Leu Pro Glu Arg Leu Leu Asn Ile Thr
290 295 300
Lys Gln Ser Phe Pro Glu Leu His Leu Thr Lys Glu Asp Cys Met Val
305 310 315 320
Lys Lys Trp Ile Glu Ser Ser Val Phe Trp Ala Asn Tyr Pro Glu Lys
325 330 335
Ala Pro Ile Glu Leu Leu Leu Lys Arg Val Ser Thr Asn Glu Tyr Tyr
340 345 350
Trp Lys Arg Thr Ser Asp Phe Val Gln Ala Pro Ile Ser Lys Gln Gly
355 360 365
Leu Ala Lys Ile Phe Gln Thr Met Ile Asp His Ser Pro Leu Pro Arg
370 375 380
Arg Val Trp Met Gln Trp Asn Pro Trp Gly Gly Lys Met Ala Glu Ile
385 390 395 400
Ala Ser Asp Ala Thr Ala Phe Val His Arg Gly Gly Asn Val Phe Met
405 410 415
Ile Glu His Phe Met Asn Trp Tyr Arg Pro Gly Asp Glu Leu Glu Glu
420 425 430
Lys Phe Leu Ala Ile Ala Arg Ser Phe Lys Glu Ala Met Ala Pro Phe
435 440 445
Val Ser Lys Asn Pro Arg Glu Ala Phe Phe Asn Tyr Arg Asp Val Asp
450 455 460
Ile Gly Ile Thr Thr Pro Gly Tyr Asn Ala Thr Tyr Glu Gly Ala Lys
465 470 475 480
Val Tyr Gly Asp Ser Tyr Phe Lys Gly Asn Tyr Leu Arg Leu Val Lys
485 490 495
Ile Lys Ala Arg Phe Asp Arg Thr Asn Phe Phe Arg Ser Gln Gln Gly
500 505 510
Ile Pro Val Leu Ala Trp Ser His Pro Gln Phe Glu Lys
515 520 525
<210> 42
<211> 1632
<212> DNA
<213> 人工序列
<220>
<223> CBS59-O64745-截断alpha因子-无信号肽-strep标签
<220>
<221> CDS
<222> (1)..(1632)
<400> 42
atg aga ttt cct tcc atc ttc acg gct gtg cta ttt gca gca tcc tcc 48
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser
1 5 10 15
gca ctt gca atg tct cca cca tcc ttg tct att cca gaa cat ttc ttg 96
Ala Leu Ala Met Ser Pro Pro Ser Leu Ser Ile Pro Glu His Phe Leu
20 25 30
aga tgc ttg gat acc caa cca tct gat cat ggt tct cca aat tct aga 144
Arg Cys Leu Asp Thr Gln Pro Ser Asp His Gly Ser Pro Asn Ser Arg
35 40 45
act gcc gtt att cca acg aac tct tct ttt tct acc aac ttg atg aac 192
Thr Ala Val Ile Pro Thr Asn Ser Ser Phe Ser Thr Asn Leu Met Asn
50 55 60
ggc gtc aga aat ttg aga ttc gct tct gtt tct act agg aag cca gaa 240
Gly Val Arg Asn Leu Arg Phe Ala Ser Val Ser Thr Arg Lys Pro Glu
65 70 75 80
gtt ata gtt gct gct gtt act gaa acc cat att aga gct acc att tct 288
Val Ile Val Ala Ala Val Thr Glu Thr His Ile Arg Ala Thr Ile Ser
85 90 95
tgc tgc aag ttg ttg aac ttg gaa ttg aga att aga tcc ggt ggt cat 336
Cys Cys Lys Leu Leu Asn Leu Glu Leu Arg Ile Arg Ser Gly Gly His
100 105 110
gat tac gaa ggt ttc tct tat act tct cca gtt cca ttc gtt atc ctg 384
Asp Tyr Glu Gly Phe Ser Tyr Thr Ser Pro Val Pro Phe Val Ile Leu
115 120 125
gac atg tac aac ttc aac aag atc gac att aac atg aag gac gaa acc 432
Asp Met Tyr Asn Phe Asn Lys Ile Asp Ile Asn Met Lys Asp Glu Thr
130 135 140
gtc tgg att caa tct ggt gct tct ttg ggt cag ttg tac tac aat att 480
Val Trp Ile Gln Ser Gly Ala Ser Leu Gly Gln Leu Tyr Tyr Asn Ile
145 150 155 160
gcc tcc aag tct aag gtt cat gct ttt cca gct ggt gtt tgt cca aaa 528
Ala Ser Lys Ser Lys Val His Ala Phe Pro Ala Gly Val Cys Pro Lys
165 170 175
gtt ggt gct ggt ggt cat ttt tct ggt ggt ggt ttt ggt aat ttg atg 576
Val Gly Ala Gly Gly His Phe Ser Gly Gly Gly Phe Gly Asn Leu Met
180 185 190
agg aaa tac ggt ttg tcc atc gat cat atc att gac gcc caa att atg 624
Arg Lys Tyr Gly Leu Ser Ile Asp His Ile Ile Asp Ala Gln Ile Met
195 200 205
gat gct aac ggt aag gtt tac aga aac aga caa gct atg ggt gaa gat 672
Asp Ala Asn Gly Lys Val Tyr Arg Asn Arg Gln Ala Met Gly Glu Asp
210 215 220
gtt ttc tgg gct att aga ggt ggt ggt ggc ggt tct tat ggt gtt att 720
Val Phe Trp Ala Ile Arg Gly Gly Gly Gly Gly Ser Tyr Gly Val Ile
225 230 235 240
ttg gct tgg aag att aag ttg gtt agg gtt cca gaa aag gtc acc gtt 768
Leu Ala Trp Lys Ile Lys Leu Val Arg Val Pro Glu Lys Val Thr Val
245 250 255
ttt aag ttg gaa aga acc gtt aga gaa ggt gcc gtt gat ttg gtt cat 816
Phe Lys Leu Glu Arg Thr Val Arg Glu Gly Ala Val Asp Leu Val His
260 265 270
aag tgg caa caa gtt gcc cca gtt att gat aga gat ttg ttc atc aga 864
Lys Trp Gln Gln Val Ala Pro Val Ile Asp Arg Asp Leu Phe Ile Arg
275 280 285
ttg gag atc aag ccc atc aac aga aag att tct aag ggt aag acc atc 912
Leu Glu Ile Lys Pro Ile Asn Arg Lys Ile Ser Lys Gly Lys Thr Ile
290 295 300
aag gtg tcc ttc att ggt atg ttt ttg ggt ttg cca gaa cgt ttg ttg 960
Lys Val Ser Phe Ile Gly Met Phe Leu Gly Leu Pro Glu Arg Leu Leu
305 310 315 320
aac atc act aag caa tct ttc cca gaa ctg cac ttg aca aaa gaa gat 1008
Asn Ile Thr Lys Gln Ser Phe Pro Glu Leu His Leu Thr Lys Glu Asp
325 330 335
tgc atg gtt aag aag tgg atc gag tca tct gtt ttt tgg gca aat tac 1056
Cys Met Val Lys Lys Trp Ile Glu Ser Ser Val Phe Trp Ala Asn Tyr
340 345 350
cca gaa aaa gcc cca att gag ttg ttg ttg aaa aga gtc tct acc aac 1104
Pro Glu Lys Ala Pro Ile Glu Leu Leu Leu Lys Arg Val Ser Thr Asn
355 360 365
gag tac tac tgg aag aga act tct gat ttt gtt cag gcc cca att tct 1152
Glu Tyr Tyr Trp Lys Arg Thr Ser Asp Phe Val Gln Ala Pro Ile Ser
370 375 380
aaa caa ggt ttg gct aag att ttc cag acc atg att gat cat tct cca 1200
Lys Gln Gly Leu Ala Lys Ile Phe Gln Thr Met Ile Asp His Ser Pro
385 390 395 400
ttg cct aga aga gtt tgg atg caa tgg aat cct tgg ggt ggt aaa atg 1248
Leu Pro Arg Arg Val Trp Met Gln Trp Asn Pro Trp Gly Gly Lys Met
405 410 415
gct gaa att gct tct gat gct act gcc ttt gtt cac aga ggt ggt aat 1296
Ala Glu Ile Ala Ser Asp Ala Thr Ala Phe Val His Arg Gly Gly Asn
420 425 430
gtt ttc atg atc gaa cat ttc atg aac tgg tac aga cca ggt gat gaa 1344
Val Phe Met Ile Glu His Phe Met Asn Trp Tyr Arg Pro Gly Asp Glu
435 440 445
ttg gaa gaa aag ttc ttg gct att gcc agg tct ttc aaa gaa gct atg 1392
Leu Glu Glu Lys Phe Leu Ala Ile Ala Arg Ser Phe Lys Glu Ala Met
450 455 460
gct cca ttt gtt tct aag aat cca aga gaa gcc ttc ttc aac tac aga 1440
Ala Pro Phe Val Ser Lys Asn Pro Arg Glu Ala Phe Phe Asn Tyr Arg
465 470 475 480
gat gtt gat att ggt atc act acc cca ggt tac aat gct act tat gaa 1488
Asp Val Asp Ile Gly Ile Thr Thr Pro Gly Tyr Asn Ala Thr Tyr Glu
485 490 495
ggt gct aaa gtt tac ggc gat tct tac ttt aag ggt aac tac ttg agg 1536
Gly Ala Lys Val Tyr Gly Asp Ser Tyr Phe Lys Gly Asn Tyr Leu Arg
500 505 510
ttg gtt aag atc aag gct aga ttc gac aga acg aac ttc ttc aga tct 1584
Leu Val Lys Ile Lys Ala Arg Phe Asp Arg Thr Asn Phe Phe Arg Ser
515 520 525
caa caa ggt att cca gtt ttg gcc tgg tca cat cca caa ttt gaa aag 1632
Gln Gln Gly Ile Pro Val Leu Ala Trp Ser His Pro Gln Phe Glu Lys
530 535 540
<210> 43
<211> 544
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 43
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser
1 5 10 15
Ala Leu Ala Met Ser Pro Pro Ser Leu Ser Ile Pro Glu His Phe Leu
20 25 30
Arg Cys Leu Asp Thr Gln Pro Ser Asp His Gly Ser Pro Asn Ser Arg
35 40 45
Thr Ala Val Ile Pro Thr Asn Ser Ser Phe Ser Thr Asn Leu Met Asn
50 55 60
Gly Val Arg Asn Leu Arg Phe Ala Ser Val Ser Thr Arg Lys Pro Glu
65 70 75 80
Val Ile Val Ala Ala Val Thr Glu Thr His Ile Arg Ala Thr Ile Ser
85 90 95
Cys Cys Lys Leu Leu Asn Leu Glu Leu Arg Ile Arg Ser Gly Gly His
100 105 110
Asp Tyr Glu Gly Phe Ser Tyr Thr Ser Pro Val Pro Phe Val Ile Leu
115 120 125
Asp Met Tyr Asn Phe Asn Lys Ile Asp Ile Asn Met Lys Asp Glu Thr
130 135 140
Val Trp Ile Gln Ser Gly Ala Ser Leu Gly Gln Leu Tyr Tyr Asn Ile
145 150 155 160
Ala Ser Lys Ser Lys Val His Ala Phe Pro Ala Gly Val Cys Pro Lys
165 170 175
Val Gly Ala Gly Gly His Phe Ser Gly Gly Gly Phe Gly Asn Leu Met
180 185 190
Arg Lys Tyr Gly Leu Ser Ile Asp His Ile Ile Asp Ala Gln Ile Met
195 200 205
Asp Ala Asn Gly Lys Val Tyr Arg Asn Arg Gln Ala Met Gly Glu Asp
210 215 220
Val Phe Trp Ala Ile Arg Gly Gly Gly Gly Gly Ser Tyr Gly Val Ile
225 230 235 240
Leu Ala Trp Lys Ile Lys Leu Val Arg Val Pro Glu Lys Val Thr Val
245 250 255
Phe Lys Leu Glu Arg Thr Val Arg Glu Gly Ala Val Asp Leu Val His
260 265 270
Lys Trp Gln Gln Val Ala Pro Val Ile Asp Arg Asp Leu Phe Ile Arg
275 280 285
Leu Glu Ile Lys Pro Ile Asn Arg Lys Ile Ser Lys Gly Lys Thr Ile
290 295 300
Lys Val Ser Phe Ile Gly Met Phe Leu Gly Leu Pro Glu Arg Leu Leu
305 310 315 320
Asn Ile Thr Lys Gln Ser Phe Pro Glu Leu His Leu Thr Lys Glu Asp
325 330 335
Cys Met Val Lys Lys Trp Ile Glu Ser Ser Val Phe Trp Ala Asn Tyr
340 345 350
Pro Glu Lys Ala Pro Ile Glu Leu Leu Leu Lys Arg Val Ser Thr Asn
355 360 365
Glu Tyr Tyr Trp Lys Arg Thr Ser Asp Phe Val Gln Ala Pro Ile Ser
370 375 380
Lys Gln Gly Leu Ala Lys Ile Phe Gln Thr Met Ile Asp His Ser Pro
385 390 395 400
Leu Pro Arg Arg Val Trp Met Gln Trp Asn Pro Trp Gly Gly Lys Met
405 410 415
Ala Glu Ile Ala Ser Asp Ala Thr Ala Phe Val His Arg Gly Gly Asn
420 425 430
Val Phe Met Ile Glu His Phe Met Asn Trp Tyr Arg Pro Gly Asp Glu
435 440 445
Leu Glu Glu Lys Phe Leu Ala Ile Ala Arg Ser Phe Lys Glu Ala Met
450 455 460
Ala Pro Phe Val Ser Lys Asn Pro Arg Glu Ala Phe Phe Asn Tyr Arg
465 470 475 480
Asp Val Asp Ile Gly Ile Thr Thr Pro Gly Tyr Asn Ala Thr Tyr Glu
485 490 495
Gly Ala Lys Val Tyr Gly Asp Ser Tyr Phe Lys Gly Asn Tyr Leu Arg
500 505 510
Leu Val Lys Ile Lys Ala Arg Phe Asp Arg Thr Asn Phe Phe Arg Ser
515 520 525
Gln Gln Gly Ile Pro Val Leu Ala Trp Ser His Pro Gln Phe Glu Lys
530 535 540
<210> 44
<211> 1842
<212> DNA
<213> 人工序列
<220>
<223> CBS59-O64745-全长alpha因子-无信号肽-strep标签
<220>
<221> CDS
<222> (1)..(1842)
<400> 44
atg aga ttt cct tca att ttt act gct gtt tta ttc gca gca tcc tcc 48
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser
1 5 10 15
gca tta gct gct cca gtc aac act aca aca gaa gat gaa acg gca caa 96
Ala Leu Ala Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln
20 25 30
att ccg gct gaa gct gtc atc ggt tac tca gat tta gaa ggg gat ttc 144
Ile Pro Ala Glu Ala Val Ile Gly Tyr Ser Asp Leu Glu Gly Asp Phe
35 40 45
gat gtt gct gtt ttg cca ttt tcc aac agc aca aat aac ggg tta ttg 192
Asp Val Ala Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu
50 55 60
ttt ata aat act act att gcc agc att gct gct aaa gaa gaa ggg gta 240
Phe Ile Asn Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val
65 70 75 80
tct ctc gag aaa aga gag gct gaa gct atg tct cca cca tcc ttg tct 288
Ser Leu Glu Lys Arg Glu Ala Glu Ala Met Ser Pro Pro Ser Leu Ser
85 90 95
att cca gaa cat ttc ttg aga tgc ttg gat acc caa cca tct gat cat 336
Ile Pro Glu His Phe Leu Arg Cys Leu Asp Thr Gln Pro Ser Asp His
100 105 110
ggt tct cca aat tct aga act gcc gtt att cca acg aac tct tct ttt 384
Gly Ser Pro Asn Ser Arg Thr Ala Val Ile Pro Thr Asn Ser Ser Phe
115 120 125
tct acc aac ttg atg aac ggc gtc aga aat ttg aga ttc gct tct gtt 432
Ser Thr Asn Leu Met Asn Gly Val Arg Asn Leu Arg Phe Ala Ser Val
130 135 140
tct act agg aag cca gaa gtt ata gtt gct gct gtt act gaa acc cat 480
Ser Thr Arg Lys Pro Glu Val Ile Val Ala Ala Val Thr Glu Thr His
145 150 155 160
att aga gct acc att tct tgc tgc aag ttg ttg aac ttg gaa ttg aga 528
Ile Arg Ala Thr Ile Ser Cys Cys Lys Leu Leu Asn Leu Glu Leu Arg
165 170 175
att aga tcc ggt ggt cat gat tac gaa ggt ttc tct tat act tct cca 576
Ile Arg Ser Gly Gly His Asp Tyr Glu Gly Phe Ser Tyr Thr Ser Pro
180 185 190
gtt cca ttc gtt atc ctg gac atg tac aac ttc aac aag atc gac att 624
Val Pro Phe Val Ile Leu Asp Met Tyr Asn Phe Asn Lys Ile Asp Ile
195 200 205
aac atg aag gac gaa acc gtc tgg att caa tct ggt gct tct ttg ggt 672
Asn Met Lys Asp Glu Thr Val Trp Ile Gln Ser Gly Ala Ser Leu Gly
210 215 220
cag ttg tac tac aat att gcc tcc aag tct aag gtt cat gct ttt cca 720
Gln Leu Tyr Tyr Asn Ile Ala Ser Lys Ser Lys Val His Ala Phe Pro
225 230 235 240
gct ggt gtt tgt cca aaa gtt ggt gct ggt ggt cat ttt tct ggt ggt 768
Ala Gly Val Cys Pro Lys Val Gly Ala Gly Gly His Phe Ser Gly Gly
245 250 255
ggt ttt ggt aat ttg atg agg aaa tac ggt ttg tcc atc gat cat atc 816
Gly Phe Gly Asn Leu Met Arg Lys Tyr Gly Leu Ser Ile Asp His Ile
260 265 270
att gac gcc caa att atg gat gct aac ggt aag gtt tac aga aac aga 864
Ile Asp Ala Gln Ile Met Asp Ala Asn Gly Lys Val Tyr Arg Asn Arg
275 280 285
caa gct atg ggt gaa gat gtt ttc tgg gct att aga ggt ggt ggt ggc 912
Gln Ala Met Gly Glu Asp Val Phe Trp Ala Ile Arg Gly Gly Gly Gly
290 295 300
ggt tct tat ggt gtt att ttg gct tgg aag att aag ttg gtt agg gtt 960
Gly Ser Tyr Gly Val Ile Leu Ala Trp Lys Ile Lys Leu Val Arg Val
305 310 315 320
cca gaa aag gtc acc gtt ttt aag ttg gaa aga acc gtt aga gaa ggt 1008
Pro Glu Lys Val Thr Val Phe Lys Leu Glu Arg Thr Val Arg Glu Gly
325 330 335
gcc gtt gat ttg gtt cat aag tgg caa caa gtt gcc cca gtt att gat 1056
Ala Val Asp Leu Val His Lys Trp Gln Gln Val Ala Pro Val Ile Asp
340 345 350
aga gat ttg ttc atc aga ttg gag atc aag ccc atc aac aga aag att 1104
Arg Asp Leu Phe Ile Arg Leu Glu Ile Lys Pro Ile Asn Arg Lys Ile
355 360 365
tct aag ggt aag acc atc aag gtg tcc ttc att ggt atg ttt ttg ggt 1152
Ser Lys Gly Lys Thr Ile Lys Val Ser Phe Ile Gly Met Phe Leu Gly
370 375 380
ttg cca gaa cgt ttg ttg aac atc act aag caa tct ttc cca gaa ctg 1200
Leu Pro Glu Arg Leu Leu Asn Ile Thr Lys Gln Ser Phe Pro Glu Leu
385 390 395 400
cac ttg aca aaa gaa gat tgc atg gtt aag aag tgg atc gag tca tct 1248
His Leu Thr Lys Glu Asp Cys Met Val Lys Lys Trp Ile Glu Ser Ser
405 410 415
gtt ttt tgg gca aat tac cca gaa aaa gcc cca att gag ttg ttg ttg 1296
Val Phe Trp Ala Asn Tyr Pro Glu Lys Ala Pro Ile Glu Leu Leu Leu
420 425 430
aaa aga gtc tct acc aac gag tac tac tgg aag aga act tct gat ttt 1344
Lys Arg Val Ser Thr Asn Glu Tyr Tyr Trp Lys Arg Thr Ser Asp Phe
435 440 445
gtt cag gcc cca att tct aaa caa ggt ttg gct aag att ttc cag acc 1392
Val Gln Ala Pro Ile Ser Lys Gln Gly Leu Ala Lys Ile Phe Gln Thr
450 455 460
atg att gat cat tct cca ttg cct aga aga gtt tgg atg caa tgg aat 1440
Met Ile Asp His Ser Pro Leu Pro Arg Arg Val Trp Met Gln Trp Asn
465 470 475 480
cct tgg ggt ggt aaa atg gct gaa att gct tct gat gct act gcc ttt 1488
Pro Trp Gly Gly Lys Met Ala Glu Ile Ala Ser Asp Ala Thr Ala Phe
485 490 495
gtt cac aga ggt ggt aat gtt ttc atg atc gaa cat ttc atg aac tgg 1536
Val His Arg Gly Gly Asn Val Phe Met Ile Glu His Phe Met Asn Trp
500 505 510
tac aga cca ggt gat gaa ttg gaa gaa aag ttc ttg gct att gcc agg 1584
Tyr Arg Pro Gly Asp Glu Leu Glu Glu Lys Phe Leu Ala Ile Ala Arg
515 520 525
tct ttc aaa gaa gct atg gct cca ttt gtt tct aag aat cca aga gaa 1632
Ser Phe Lys Glu Ala Met Ala Pro Phe Val Ser Lys Asn Pro Arg Glu
530 535 540
gcc ttc ttc aac tac aga gat gtt gat att ggt atc act acc cca ggt 1680
Ala Phe Phe Asn Tyr Arg Asp Val Asp Ile Gly Ile Thr Thr Pro Gly
545 550 555 560
tac aat gct act tat gaa ggt gct aaa gtt tac ggc gat tct tac ttt 1728
Tyr Asn Ala Thr Tyr Glu Gly Ala Lys Val Tyr Gly Asp Ser Tyr Phe
565 570 575
aag ggt aac tac ttg agg ttg gtt aag atc aag gct aga ttc gac aga 1776
Lys Gly Asn Tyr Leu Arg Leu Val Lys Ile Lys Ala Arg Phe Asp Arg
580 585 590
acg aac ttc ttc aga tct caa caa ggt att cca gtt ttg gcc tgg tca 1824
Thr Asn Phe Phe Arg Ser Gln Gln Gly Ile Pro Val Leu Ala Trp Ser
595 600 605
cat cca caa ttt gaa aag 1842
His Pro Gln Phe Glu Lys
610
<210> 45
<211> 614
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 45
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser
1 5 10 15
Ala Leu Ala Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln
20 25 30
Ile Pro Ala Glu Ala Val Ile Gly Tyr Ser Asp Leu Glu Gly Asp Phe
35 40 45
Asp Val Ala Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu
50 55 60
Phe Ile Asn Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val
65 70 75 80
Ser Leu Glu Lys Arg Glu Ala Glu Ala Met Ser Pro Pro Ser Leu Ser
85 90 95
Ile Pro Glu His Phe Leu Arg Cys Leu Asp Thr Gln Pro Ser Asp His
100 105 110
Gly Ser Pro Asn Ser Arg Thr Ala Val Ile Pro Thr Asn Ser Ser Phe
115 120 125
Ser Thr Asn Leu Met Asn Gly Val Arg Asn Leu Arg Phe Ala Ser Val
130 135 140
Ser Thr Arg Lys Pro Glu Val Ile Val Ala Ala Val Thr Glu Thr His
145 150 155 160
Ile Arg Ala Thr Ile Ser Cys Cys Lys Leu Leu Asn Leu Glu Leu Arg
165 170 175
Ile Arg Ser Gly Gly His Asp Tyr Glu Gly Phe Ser Tyr Thr Ser Pro
180 185 190
Val Pro Phe Val Ile Leu Asp Met Tyr Asn Phe Asn Lys Ile Asp Ile
195 200 205
Asn Met Lys Asp Glu Thr Val Trp Ile Gln Ser Gly Ala Ser Leu Gly
210 215 220
Gln Leu Tyr Tyr Asn Ile Ala Ser Lys Ser Lys Val His Ala Phe Pro
225 230 235 240
Ala Gly Val Cys Pro Lys Val Gly Ala Gly Gly His Phe Ser Gly Gly
245 250 255
Gly Phe Gly Asn Leu Met Arg Lys Tyr Gly Leu Ser Ile Asp His Ile
260 265 270
Ile Asp Ala Gln Ile Met Asp Ala Asn Gly Lys Val Tyr Arg Asn Arg
275 280 285
Gln Ala Met Gly Glu Asp Val Phe Trp Ala Ile Arg Gly Gly Gly Gly
290 295 300
Gly Ser Tyr Gly Val Ile Leu Ala Trp Lys Ile Lys Leu Val Arg Val
305 310 315 320
Pro Glu Lys Val Thr Val Phe Lys Leu Glu Arg Thr Val Arg Glu Gly
325 330 335
Ala Val Asp Leu Val His Lys Trp Gln Gln Val Ala Pro Val Ile Asp
340 345 350
Arg Asp Leu Phe Ile Arg Leu Glu Ile Lys Pro Ile Asn Arg Lys Ile
355 360 365
Ser Lys Gly Lys Thr Ile Lys Val Ser Phe Ile Gly Met Phe Leu Gly
370 375 380
Leu Pro Glu Arg Leu Leu Asn Ile Thr Lys Gln Ser Phe Pro Glu Leu
385 390 395 400
His Leu Thr Lys Glu Asp Cys Met Val Lys Lys Trp Ile Glu Ser Ser
405 410 415
Val Phe Trp Ala Asn Tyr Pro Glu Lys Ala Pro Ile Glu Leu Leu Leu
420 425 430
Lys Arg Val Ser Thr Asn Glu Tyr Tyr Trp Lys Arg Thr Ser Asp Phe
435 440 445
Val Gln Ala Pro Ile Ser Lys Gln Gly Leu Ala Lys Ile Phe Gln Thr
450 455 460
Met Ile Asp His Ser Pro Leu Pro Arg Arg Val Trp Met Gln Trp Asn
465 470 475 480
Pro Trp Gly Gly Lys Met Ala Glu Ile Ala Ser Asp Ala Thr Ala Phe
485 490 495
Val His Arg Gly Gly Asn Val Phe Met Ile Glu His Phe Met Asn Trp
500 505 510
Tyr Arg Pro Gly Asp Glu Leu Glu Glu Lys Phe Leu Ala Ile Ala Arg
515 520 525
Ser Phe Lys Glu Ala Met Ala Pro Phe Val Ser Lys Asn Pro Arg Glu
530 535 540
Ala Phe Phe Asn Tyr Arg Asp Val Asp Ile Gly Ile Thr Thr Pro Gly
545 550 555 560
Tyr Asn Ala Thr Tyr Glu Gly Ala Lys Val Tyr Gly Asp Ser Tyr Phe
565 570 575
Lys Gly Asn Tyr Leu Arg Leu Val Lys Ile Lys Ala Arg Phe Asp Arg
580 585 590
Thr Asn Phe Phe Arg Ser Gln Gln Gly Ile Pro Val Leu Ala Trp Ser
595 600 605
His Pro Gln Phe Glu Lys
610
<210> 46
<211> 1605
<212> DNA
<213> 人工序列
<220>
<223> CBS69-P93479
<220>
<221> CDS
<222> (1)..(1605)
<400> 46
atg atg tgc aga tct ttg acc ttg agg ttc ttc ttg ttc atc gtt ttg 48
Met Met Cys Arg Ser Leu Thr Leu Arg Phe Phe Leu Phe Ile Val Leu
1 5 10 15
ttg caa acc tgt gtt aga ggt ggt gat gtt aac gat aac ttg ttg tcc 96
Leu Gln Thr Cys Val Arg Gly Gly Asp Val Asn Asp Asn Leu Leu Ser
20 25 30
tct tgc ttg aac tct cat ggt gtt cat aac ttc act act ttg tct acc 144
Ser Cys Leu Asn Ser His Gly Val His Asn Phe Thr Thr Leu Ser Thr
35 40 45
gat acc aac tcc gat tac ttc aag ttg ttg cat gct tct atg caa aac 192
Asp Thr Asn Ser Asp Tyr Phe Lys Leu Leu His Ala Ser Met Gln Asn
50 55 60
cct ttg ttt gct aag cca acc gtt tct aag cca tct ttt atc gtt atg 240
Pro Leu Phe Ala Lys Pro Thr Val Ser Lys Pro Ser Phe Ile Val Met
65 70 75 80
cca ggc tcc aaa gaa gaa ttg tca tct act gtt cat tgc tgc act aga 288
Pro Gly Ser Lys Glu Glu Leu Ser Ser Thr Val His Cys Cys Thr Arg
85 90 95
gaa tct tgg acc att aga ttg aga tct ggt ggt cat tct tac gag ggt 336
Glu Ser Trp Thr Ile Arg Leu Arg Ser Gly Gly His Ser Tyr Glu Gly
100 105 110
ttg tct tat act gct gat act cca ttc gtt atc gtc gac atg atg aac 384
Leu Ser Tyr Thr Ala Asp Thr Pro Phe Val Ile Val Asp Met Met Asn
115 120 125
ttg aac aga atc tcc atc gat gtc ttg tct gaa act gct tgg gtt gaa 432
Leu Asn Arg Ile Ser Ile Asp Val Leu Ser Glu Thr Ala Trp Val Glu
130 135 140
tct ggt gct act ttg ggt gaa ctg tat tat gct att gct caa tcc act 480
Ser Gly Ala Thr Leu Gly Glu Leu Tyr Tyr Ala Ile Ala Gln Ser Thr
145 150 155 160
gat acc ttg ggt ttt act gct ggt tgg tgt cca act gtt ggt tca ggt 528
Asp Thr Leu Gly Phe Thr Ala Gly Trp Cys Pro Thr Val Gly Ser Gly
165 170 175
ggt cat att tct ggt ggt ggt ttt ggt atg atg tct aga aag tat ggt 576
Gly His Ile Ser Gly Gly Gly Phe Gly Met Met Ser Arg Lys Tyr Gly
180 185 190
ttg gct gct gat aac gtt gtt gat gcc att ttg att gat tcc aac ggt 624
Leu Ala Ala Asp Asn Val Val Asp Ala Ile Leu Ile Asp Ser Asn Gly
195 200 205
gct atc ttg gac aga gaa aaa atg ggt gat gat gtt ttc tgg gct att 672
Ala Ile Leu Asp Arg Glu Lys Met Gly Asp Asp Val Phe Trp Ala Ile
210 215 220
aga ggc ggt ggt ggt ggc gtt tgg ggt gca atc tat gct tgg aaa atc 720
Arg Gly Gly Gly Gly Gly Val Trp Gly Ala Ile Tyr Ala Trp Lys Ile
225 230 235 240
aag tta ttg cca gtg cca gaa aag ttg acc gtt ttc aga gtt act aag 768
Lys Leu Leu Pro Val Pro Glu Lys Leu Thr Val Phe Arg Val Thr Lys
245 250 255
aac gtc ggt att gaa gat gcc tct tct ttg ttg cac aaa tgg caa tat 816
Asn Val Gly Ile Glu Asp Ala Ser Ser Leu Leu His Lys Trp Gln Tyr
260 265 270
gtt gcc gat gaa ttg gat gag gat ttc acc gtt tct gtt tta ggt ggt 864
Val Ala Asp Glu Leu Asp Glu Asp Phe Thr Val Ser Val Leu Gly Gly
275 280 285
gtt aat ggt aat gat gcc tgg ttg atg ttc ttg ggt tta cat ttg ggt 912
Val Asn Gly Asn Asp Ala Trp Leu Met Phe Leu Gly Leu His Leu Gly
290 295 300
aga aag gat gct gcc aag acc att att gac gaa aag ttt cca gaa ttg 960
Arg Lys Asp Ala Ala Lys Thr Ile Ile Asp Glu Lys Phe Pro Glu Leu
305 310 315 320
ggc cta gtc gac aaa gaa ttt caa gaa atg tct tgg ggt gaa tcg atg 1008
Gly Leu Val Asp Lys Glu Phe Gln Glu Met Ser Trp Gly Glu Ser Met
325 330 335
gct ttt ttg tct ggt ttg gat acc atc tct gaa ctg aac aac aga ttc 1056
Ala Phe Leu Ser Gly Leu Asp Thr Ile Ser Glu Leu Asn Asn Arg Phe
340 345 350
ttg aag ttc gac gaa aga gct ttc aag acc aag gtt gat ttc acc aaa 1104
Leu Lys Phe Asp Glu Arg Ala Phe Lys Thr Lys Val Asp Phe Thr Lys
355 360 365
gtt tcc gtt cca ttg aac gtt ttt aga cac gct ttg gaa atg ttg tct 1152
Val Ser Val Pro Leu Asn Val Phe Arg His Ala Leu Glu Met Leu Ser
370 375 380
gaa caa cca ggt ggt ttc att gct ttg aat ggt ttc ggt ggt aag atg 1200
Glu Gln Pro Gly Gly Phe Ile Ala Leu Asn Gly Phe Gly Gly Lys Met
385 390 395 400
tcc gaa att tct act gac ttt acc cca ttt cca cac aga aaa ggt acg 1248
Ser Glu Ile Ser Thr Asp Phe Thr Pro Phe Pro His Arg Lys Gly Thr
405 410 415
aag ttg atg ttt gag tac att att gcc tgg aac cag gac gaa gaa tct 1296
Lys Leu Met Phe Glu Tyr Ile Ile Ala Trp Asn Gln Asp Glu Glu Ser
420 425 430
aag att ggt gaa ttt tct gaa tgg ctg gcc aag ttc tat gat tac ttg 1344
Lys Ile Gly Glu Phe Ser Glu Trp Leu Ala Lys Phe Tyr Asp Tyr Leu
435 440 445
gaa cca ttc gtt tcc aaa gaa cct aga gtt ggt tac gtt aac cac atc 1392
Glu Pro Phe Val Ser Lys Glu Pro Arg Val Gly Tyr Val Asn His Ile
450 455 460
gat ttg gat att ggt ggt atc gat tgg agg aac aaa tct tct act acc 1440
Asp Leu Asp Ile Gly Gly Ile Asp Trp Arg Asn Lys Ser Ser Thr Thr
465 470 475 480
aac gct gtt gaa att gct aga aac tgg ggt gaa aga tac ttc agc tct 1488
Asn Ala Val Glu Ile Ala Arg Asn Trp Gly Glu Arg Tyr Phe Ser Ser
485 490 495
aac tac gaa aga ttg gtt aag gcc aag act ttg atc gat cca aac aac 1536
Asn Tyr Glu Arg Leu Val Lys Ala Lys Thr Leu Ile Asp Pro Asn Asn
500 505 510
gtt ttc aac cat cca caa tct att cca cca atg atg aag ttt gaa gag 1584
Val Phe Asn His Pro Gln Ser Ile Pro Pro Met Met Lys Phe Glu Glu
515 520 525
atc tac atg ctg aaa gaa ttg 1605
Ile Tyr Met Leu Lys Glu Leu
530 535
<210> 47
<211> 535
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 47
Met Met Cys Arg Ser Leu Thr Leu Arg Phe Phe Leu Phe Ile Val Leu
1 5 10 15
Leu Gln Thr Cys Val Arg Gly Gly Asp Val Asn Asp Asn Leu Leu Ser
20 25 30
Ser Cys Leu Asn Ser His Gly Val His Asn Phe Thr Thr Leu Ser Thr
35 40 45
Asp Thr Asn Ser Asp Tyr Phe Lys Leu Leu His Ala Ser Met Gln Asn
50 55 60
Pro Leu Phe Ala Lys Pro Thr Val Ser Lys Pro Ser Phe Ile Val Met
65 70 75 80
Pro Gly Ser Lys Glu Glu Leu Ser Ser Thr Val His Cys Cys Thr Arg
85 90 95
Glu Ser Trp Thr Ile Arg Leu Arg Ser Gly Gly His Ser Tyr Glu Gly
100 105 110
Leu Ser Tyr Thr Ala Asp Thr Pro Phe Val Ile Val Asp Met Met Asn
115 120 125
Leu Asn Arg Ile Ser Ile Asp Val Leu Ser Glu Thr Ala Trp Val Glu
130 135 140
Ser Gly Ala Thr Leu Gly Glu Leu Tyr Tyr Ala Ile Ala Gln Ser Thr
145 150 155 160
Asp Thr Leu Gly Phe Thr Ala Gly Trp Cys Pro Thr Val Gly Ser Gly
165 170 175
Gly His Ile Ser Gly Gly Gly Phe Gly Met Met Ser Arg Lys Tyr Gly
180 185 190
Leu Ala Ala Asp Asn Val Val Asp Ala Ile Leu Ile Asp Ser Asn Gly
195 200 205
Ala Ile Leu Asp Arg Glu Lys Met Gly Asp Asp Val Phe Trp Ala Ile
210 215 220
Arg Gly Gly Gly Gly Gly Val Trp Gly Ala Ile Tyr Ala Trp Lys Ile
225 230 235 240
Lys Leu Leu Pro Val Pro Glu Lys Leu Thr Val Phe Arg Val Thr Lys
245 250 255
Asn Val Gly Ile Glu Asp Ala Ser Ser Leu Leu His Lys Trp Gln Tyr
260 265 270
Val Ala Asp Glu Leu Asp Glu Asp Phe Thr Val Ser Val Leu Gly Gly
275 280 285
Val Asn Gly Asn Asp Ala Trp Leu Met Phe Leu Gly Leu His Leu Gly
290 295 300
Arg Lys Asp Ala Ala Lys Thr Ile Ile Asp Glu Lys Phe Pro Glu Leu
305 310 315 320
Gly Leu Val Asp Lys Glu Phe Gln Glu Met Ser Trp Gly Glu Ser Met
325 330 335
Ala Phe Leu Ser Gly Leu Asp Thr Ile Ser Glu Leu Asn Asn Arg Phe
340 345 350
Leu Lys Phe Asp Glu Arg Ala Phe Lys Thr Lys Val Asp Phe Thr Lys
355 360 365
Val Ser Val Pro Leu Asn Val Phe Arg His Ala Leu Glu Met Leu Ser
370 375 380
Glu Gln Pro Gly Gly Phe Ile Ala Leu Asn Gly Phe Gly Gly Lys Met
385 390 395 400
Ser Glu Ile Ser Thr Asp Phe Thr Pro Phe Pro His Arg Lys Gly Thr
405 410 415
Lys Leu Met Phe Glu Tyr Ile Ile Ala Trp Asn Gln Asp Glu Glu Ser
420 425 430
Lys Ile Gly Glu Phe Ser Glu Trp Leu Ala Lys Phe Tyr Asp Tyr Leu
435 440 445
Glu Pro Phe Val Ser Lys Glu Pro Arg Val Gly Tyr Val Asn His Ile
450 455 460
Asp Leu Asp Ile Gly Gly Ile Asp Trp Arg Asn Lys Ser Ser Thr Thr
465 470 475 480
Asn Ala Val Glu Ile Ala Arg Asn Trp Gly Glu Arg Tyr Phe Ser Ser
485 490 495
Asn Tyr Glu Arg Leu Val Lys Ala Lys Thr Leu Ile Asp Pro Asn Asn
500 505 510
Val Phe Asn His Pro Gln Ser Ile Pro Pro Met Met Lys Phe Glu Glu
515 520 525
Ile Tyr Met Leu Lys Glu Leu
530 535
<210> 48
<211> 1539
<212> DNA
<213> 人工序列
<220>
<223> CBS69-P93479-无信号肽
<220>
<221> CDS
<222> (1)..(1539)
<400> 48
atg ggt gat gtt aac gat aac ttg ttg tcc tct tgc ttg aac tct cat 48
Met Gly Asp Val Asn Asp Asn Leu Leu Ser Ser Cys Leu Asn Ser His
1 5 10 15
ggt gtt cat aac ttc act act ttg tct acc gat acc aac tcc gat tac 96
Gly Val His Asn Phe Thr Thr Leu Ser Thr Asp Thr Asn Ser Asp Tyr
20 25 30
ttc aag ttg ttg cat gct tct atg caa aac cct ttg ttt gct aag cca 144
Phe Lys Leu Leu His Ala Ser Met Gln Asn Pro Leu Phe Ala Lys Pro
35 40 45
acc gtt tct aag cca tct ttt atc gtt atg cca ggc tcc aaa gaa gaa 192
Thr Val Ser Lys Pro Ser Phe Ile Val Met Pro Gly Ser Lys Glu Glu
50 55 60
ttg tca tct act gtt cat tgc tgc act aga gaa tct tgg acc att aga 240
Leu Ser Ser Thr Val His Cys Cys Thr Arg Glu Ser Trp Thr Ile Arg
65 70 75 80
ttg aga tct ggt ggt cat tct tac gag ggt ttg tct tat act gct gat 288
Leu Arg Ser Gly Gly His Ser Tyr Glu Gly Leu Ser Tyr Thr Ala Asp
85 90 95
act cca ttc gtt atc gtc gac atg atg aac ttg aac aga atc tcc atc 336
Thr Pro Phe Val Ile Val Asp Met Met Asn Leu Asn Arg Ile Ser Ile
100 105 110
gat gtc ttg tct gaa act gct tgg gtt gaa tct ggt gct act ttg ggt 384
Asp Val Leu Ser Glu Thr Ala Trp Val Glu Ser Gly Ala Thr Leu Gly
115 120 125
gaa ctg tat tat gct att gct caa tcc act gat acc ttg ggt ttt act 432
Glu Leu Tyr Tyr Ala Ile Ala Gln Ser Thr Asp Thr Leu Gly Phe Thr
130 135 140
gct ggt tgg tgt cca act gtt ggt tca ggt ggt cat att tct ggt ggt 480
Ala Gly Trp Cys Pro Thr Val Gly Ser Gly Gly His Ile Ser Gly Gly
145 150 155 160
ggt ttt ggt atg atg tct aga aag tat ggt ttg gct gct gat aac gtt 528
Gly Phe Gly Met Met Ser Arg Lys Tyr Gly Leu Ala Ala Asp Asn Val
165 170 175
gtt gat gcc att ttg att gat tcc aac ggt gct atc ttg gac aga gaa 576
Val Asp Ala Ile Leu Ile Asp Ser Asn Gly Ala Ile Leu Asp Arg Glu
180 185 190
aaa atg ggt gat gat gtt ttc tgg gct att aga ggc ggt ggt ggt ggc 624
Lys Met Gly Asp Asp Val Phe Trp Ala Ile Arg Gly Gly Gly Gly Gly
195 200 205
gtt tgg ggt gca atc tat gct tgg aaa atc aag tta ttg cca gtg cca 672
Val Trp Gly Ala Ile Tyr Ala Trp Lys Ile Lys Leu Leu Pro Val Pro
210 215 220
gaa aag ttg acc gtt ttc aga gtt act aag aac gtc ggt att gaa gat 720
Glu Lys Leu Thr Val Phe Arg Val Thr Lys Asn Val Gly Ile Glu Asp
225 230 235 240
gcc tct tct ttg ttg cac aaa tgg caa tat gtt gcc gat gaa ttg gat 768
Ala Ser Ser Leu Leu His Lys Trp Gln Tyr Val Ala Asp Glu Leu Asp
245 250 255
gag gat ttc acc gtt tct gtt tta ggt ggt gtt aat ggt aat gat gcc 816
Glu Asp Phe Thr Val Ser Val Leu Gly Gly Val Asn Gly Asn Asp Ala
260 265 270
tgg ttg atg ttc ttg ggt tta cat ttg ggt aga aag gat gct gcc aag 864
Trp Leu Met Phe Leu Gly Leu His Leu Gly Arg Lys Asp Ala Ala Lys
275 280 285
acc att att gac gaa aag ttt cca gaa ttg ggc cta gtc gac aaa gaa 912
Thr Ile Ile Asp Glu Lys Phe Pro Glu Leu Gly Leu Val Asp Lys Glu
290 295 300
ttt caa gaa atg tct tgg ggt gaa tcg atg gct ttt ttg tct ggt ttg 960
Phe Gln Glu Met Ser Trp Gly Glu Ser Met Ala Phe Leu Ser Gly Leu
305 310 315 320
gat acc atc tct gaa ctg aac aac aga ttc ttg aag ttc gac gaa aga 1008
Asp Thr Ile Ser Glu Leu Asn Asn Arg Phe Leu Lys Phe Asp Glu Arg
325 330 335
gct ttc aag acc aag gtt gat ttc acc aaa gtt tcc gtt cca ttg aac 1056
Ala Phe Lys Thr Lys Val Asp Phe Thr Lys Val Ser Val Pro Leu Asn
340 345 350
gtt ttt aga cac gct ttg gaa atg ttg tct gaa caa cca ggt ggt ttc 1104
Val Phe Arg His Ala Leu Glu Met Leu Ser Glu Gln Pro Gly Gly Phe
355 360 365
att gct ttg aat ggt ttc ggt ggt aag atg tcc gaa att tct act gac 1152
Ile Ala Leu Asn Gly Phe Gly Gly Lys Met Ser Glu Ile Ser Thr Asp
370 375 380
ttt acc cca ttt cca cac aga aaa ggt acg aag ttg atg ttt gag tac 1200
Phe Thr Pro Phe Pro His Arg Lys Gly Thr Lys Leu Met Phe Glu Tyr
385 390 395 400
att att gcc tgg aac cag gac gaa gaa tct aag att ggt gaa ttt tct 1248
Ile Ile Ala Trp Asn Gln Asp Glu Glu Ser Lys Ile Gly Glu Phe Ser
405 410 415
gaa tgg ctg gcc aag ttc tat gat tac ttg gaa cca ttc gtt tcc aaa 1296
Glu Trp Leu Ala Lys Phe Tyr Asp Tyr Leu Glu Pro Phe Val Ser Lys
420 425 430
gaa cct aga gtt ggt tac gtt aac cac atc gat ttg gat att ggt ggt 1344
Glu Pro Arg Val Gly Tyr Val Asn His Ile Asp Leu Asp Ile Gly Gly
435 440 445
atc gat tgg agg aac aaa tct tct act acc aac gct gtt gaa att gct 1392
Ile Asp Trp Arg Asn Lys Ser Ser Thr Thr Asn Ala Val Glu Ile Ala
450 455 460
aga aac tgg ggt gaa aga tac ttc agc tct aac tac gaa aga ttg gtt 1440
Arg Asn Trp Gly Glu Arg Tyr Phe Ser Ser Asn Tyr Glu Arg Leu Val
465 470 475 480
aag gcc aag act ttg atc gat cca aac aac gtt ttc aac cat cca caa 1488
Lys Ala Lys Thr Leu Ile Asp Pro Asn Asn Val Phe Asn His Pro Gln
485 490 495
tct att cca cca atg atg aag ttt gaa gag atc tac atg ctg aaa gaa 1536
Ser Ile Pro Pro Met Met Lys Phe Glu Glu Ile Tyr Met Leu Lys Glu
500 505 510
ttg 1539
Leu
<210> 49
<211> 513
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 49
Met Gly Asp Val Asn Asp Asn Leu Leu Ser Ser Cys Leu Asn Ser His
1 5 10 15
Gly Val His Asn Phe Thr Thr Leu Ser Thr Asp Thr Asn Ser Asp Tyr
20 25 30
Phe Lys Leu Leu His Ala Ser Met Gln Asn Pro Leu Phe Ala Lys Pro
35 40 45
Thr Val Ser Lys Pro Ser Phe Ile Val Met Pro Gly Ser Lys Glu Glu
50 55 60
Leu Ser Ser Thr Val His Cys Cys Thr Arg Glu Ser Trp Thr Ile Arg
65 70 75 80
Leu Arg Ser Gly Gly His Ser Tyr Glu Gly Leu Ser Tyr Thr Ala Asp
85 90 95
Thr Pro Phe Val Ile Val Asp Met Met Asn Leu Asn Arg Ile Ser Ile
100 105 110
Asp Val Leu Ser Glu Thr Ala Trp Val Glu Ser Gly Ala Thr Leu Gly
115 120 125
Glu Leu Tyr Tyr Ala Ile Ala Gln Ser Thr Asp Thr Leu Gly Phe Thr
130 135 140
Ala Gly Trp Cys Pro Thr Val Gly Ser Gly Gly His Ile Ser Gly Gly
145 150 155 160
Gly Phe Gly Met Met Ser Arg Lys Tyr Gly Leu Ala Ala Asp Asn Val
165 170 175
Val Asp Ala Ile Leu Ile Asp Ser Asn Gly Ala Ile Leu Asp Arg Glu
180 185 190
Lys Met Gly Asp Asp Val Phe Trp Ala Ile Arg Gly Gly Gly Gly Gly
195 200 205
Val Trp Gly Ala Ile Tyr Ala Trp Lys Ile Lys Leu Leu Pro Val Pro
210 215 220
Glu Lys Leu Thr Val Phe Arg Val Thr Lys Asn Val Gly Ile Glu Asp
225 230 235 240
Ala Ser Ser Leu Leu His Lys Trp Gln Tyr Val Ala Asp Glu Leu Asp
245 250 255
Glu Asp Phe Thr Val Ser Val Leu Gly Gly Val Asn Gly Asn Asp Ala
260 265 270
Trp Leu Met Phe Leu Gly Leu His Leu Gly Arg Lys Asp Ala Ala Lys
275 280 285
Thr Ile Ile Asp Glu Lys Phe Pro Glu Leu Gly Leu Val Asp Lys Glu
290 295 300
Phe Gln Glu Met Ser Trp Gly Glu Ser Met Ala Phe Leu Ser Gly Leu
305 310 315 320
Asp Thr Ile Ser Glu Leu Asn Asn Arg Phe Leu Lys Phe Asp Glu Arg
325 330 335
Ala Phe Lys Thr Lys Val Asp Phe Thr Lys Val Ser Val Pro Leu Asn
340 345 350
Val Phe Arg His Ala Leu Glu Met Leu Ser Glu Gln Pro Gly Gly Phe
355 360 365
Ile Ala Leu Asn Gly Phe Gly Gly Lys Met Ser Glu Ile Ser Thr Asp
370 375 380
Phe Thr Pro Phe Pro His Arg Lys Gly Thr Lys Leu Met Phe Glu Tyr
385 390 395 400
Ile Ile Ala Trp Asn Gln Asp Glu Glu Ser Lys Ile Gly Glu Phe Ser
405 410 415
Glu Trp Leu Ala Lys Phe Tyr Asp Tyr Leu Glu Pro Phe Val Ser Lys
420 425 430
Glu Pro Arg Val Gly Tyr Val Asn His Ile Asp Leu Asp Ile Gly Gly
435 440 445
Ile Asp Trp Arg Asn Lys Ser Ser Thr Thr Asn Ala Val Glu Ile Ala
450 455 460
Arg Asn Trp Gly Glu Arg Tyr Phe Ser Ser Asn Tyr Glu Arg Leu Val
465 470 475 480
Lys Ala Lys Thr Leu Ile Asp Pro Asn Asn Val Phe Asn His Pro Gln
485 490 495
Ser Ile Pro Pro Met Met Lys Phe Glu Glu Ile Tyr Met Leu Lys Glu
500 505 510
Leu
<210> 50
<211> 1563
<212> DNA
<213> 人工序列
<220>
<223> CBS69-P93479-无信号肽-strep标签
<220>
<221> CDS
<222> (1)..(1563)
<400> 50
atg ggt gat gtt aac gat aac ttg ttg tcc tct tgc ttg aac tct cat 48
Met Gly Asp Val Asn Asp Asn Leu Leu Ser Ser Cys Leu Asn Ser His
1 5 10 15
ggt gtt cat aac ttc act act ttg tct acc gat acc aac tcc gat tac 96
Gly Val His Asn Phe Thr Thr Leu Ser Thr Asp Thr Asn Ser Asp Tyr
20 25 30
ttc aag ttg ttg cat gct tct atg caa aac cct ttg ttt gct aag cca 144
Phe Lys Leu Leu His Ala Ser Met Gln Asn Pro Leu Phe Ala Lys Pro
35 40 45
acc gtt tct aag cca tct ttt atc gtt atg cca ggc tcc aaa gaa gaa 192
Thr Val Ser Lys Pro Ser Phe Ile Val Met Pro Gly Ser Lys Glu Glu
50 55 60
ttg tca tct act gtt cat tgc tgc act aga gaa tct tgg acc att aga 240
Leu Ser Ser Thr Val His Cys Cys Thr Arg Glu Ser Trp Thr Ile Arg
65 70 75 80
ttg aga tct ggt ggt cat tct tac gag ggt ttg tct tat act gct gat 288
Leu Arg Ser Gly Gly His Ser Tyr Glu Gly Leu Ser Tyr Thr Ala Asp
85 90 95
act cca ttc gtt atc gtc gac atg atg aac ttg aac aga atc tcc atc 336
Thr Pro Phe Val Ile Val Asp Met Met Asn Leu Asn Arg Ile Ser Ile
100 105 110
gat gtc ttg tct gaa act gct tgg gtt gaa tct ggt gct act ttg ggt 384
Asp Val Leu Ser Glu Thr Ala Trp Val Glu Ser Gly Ala Thr Leu Gly
115 120 125
gaa ctg tat tat gct att gct caa tcc act gat acc ttg ggt ttt act 432
Glu Leu Tyr Tyr Ala Ile Ala Gln Ser Thr Asp Thr Leu Gly Phe Thr
130 135 140
gct ggt tgg tgt cca act gtt ggt tca ggt ggt cat att tct ggt ggt 480
Ala Gly Trp Cys Pro Thr Val Gly Ser Gly Gly His Ile Ser Gly Gly
145 150 155 160
ggt ttt ggt atg atg tct aga aag tat ggt ttg gct gct gat aac gtt 528
Gly Phe Gly Met Met Ser Arg Lys Tyr Gly Leu Ala Ala Asp Asn Val
165 170 175
gtt gat gcc att ttg att gat tcc aac ggt gct atc ttg gac aga gaa 576
Val Asp Ala Ile Leu Ile Asp Ser Asn Gly Ala Ile Leu Asp Arg Glu
180 185 190
aaa atg ggt gat gat gtt ttc tgg gct att aga ggc ggt ggt ggt ggc 624
Lys Met Gly Asp Asp Val Phe Trp Ala Ile Arg Gly Gly Gly Gly Gly
195 200 205
gtt tgg ggt gca atc tat gct tgg aaa atc aag tta ttg cca gtg cca 672
Val Trp Gly Ala Ile Tyr Ala Trp Lys Ile Lys Leu Leu Pro Val Pro
210 215 220
gaa aag ttg acc gtt ttc aga gtt act aag aac gtc ggt att gaa gat 720
Glu Lys Leu Thr Val Phe Arg Val Thr Lys Asn Val Gly Ile Glu Asp
225 230 235 240
gcc tct tct ttg ttg cac aaa tgg caa tat gtt gcc gat gaa ttg gat 768
Ala Ser Ser Leu Leu His Lys Trp Gln Tyr Val Ala Asp Glu Leu Asp
245 250 255
gag gat ttc acc gtt tct gtt tta ggt ggt gtt aat ggt aat gat gcc 816
Glu Asp Phe Thr Val Ser Val Leu Gly Gly Val Asn Gly Asn Asp Ala
260 265 270
tgg ttg atg ttc ttg ggt tta cat ttg ggt aga aag gat gct gcc aag 864
Trp Leu Met Phe Leu Gly Leu His Leu Gly Arg Lys Asp Ala Ala Lys
275 280 285
acc att att gac gaa aag ttt cca gaa ttg ggc cta gtc gac aaa gaa 912
Thr Ile Ile Asp Glu Lys Phe Pro Glu Leu Gly Leu Val Asp Lys Glu
290 295 300
ttt caa gaa atg tct tgg ggt gaa tcg atg gct ttt ttg tct ggt ttg 960
Phe Gln Glu Met Ser Trp Gly Glu Ser Met Ala Phe Leu Ser Gly Leu
305 310 315 320
gat acc atc tct gaa ctg aac aac aga ttc ttg aag ttc gac gaa aga 1008
Asp Thr Ile Ser Glu Leu Asn Asn Arg Phe Leu Lys Phe Asp Glu Arg
325 330 335
gct ttc aag acc aag gtt gat ttc acc aaa gtt tcc gtt cca ttg aac 1056
Ala Phe Lys Thr Lys Val Asp Phe Thr Lys Val Ser Val Pro Leu Asn
340 345 350
gtt ttt aga cac gct ttg gaa atg ttg tct gaa caa cca ggt ggt ttc 1104
Val Phe Arg His Ala Leu Glu Met Leu Ser Glu Gln Pro Gly Gly Phe
355 360 365
att gct ttg aat ggt ttc ggt ggt aag atg tcc gaa att tct act gac 1152
Ile Ala Leu Asn Gly Phe Gly Gly Lys Met Ser Glu Ile Ser Thr Asp
370 375 380
ttt acc cca ttt cca cac aga aaa ggt acg aag ttg atg ttt gag tac 1200
Phe Thr Pro Phe Pro His Arg Lys Gly Thr Lys Leu Met Phe Glu Tyr
385 390 395 400
att att gcc tgg aac cag gac gaa gaa tct aag att ggt gaa ttt tct 1248
Ile Ile Ala Trp Asn Gln Asp Glu Glu Ser Lys Ile Gly Glu Phe Ser
405 410 415
gaa tgg ctg gcc aag ttc tat gat tac ttg gaa cca ttc gtt tcc aaa 1296
Glu Trp Leu Ala Lys Phe Tyr Asp Tyr Leu Glu Pro Phe Val Ser Lys
420 425 430
gaa cct aga gtt ggt tac gtt aac cac atc gat ttg gat att ggt ggt 1344
Glu Pro Arg Val Gly Tyr Val Asn His Ile Asp Leu Asp Ile Gly Gly
435 440 445
atc gat tgg agg aac aaa tct tct act acc aac gct gtt gaa att gct 1392
Ile Asp Trp Arg Asn Lys Ser Ser Thr Thr Asn Ala Val Glu Ile Ala
450 455 460
aga aac tgg ggt gaa aga tac ttc agc tct aac tac gaa aga ttg gtt 1440
Arg Asn Trp Gly Glu Arg Tyr Phe Ser Ser Asn Tyr Glu Arg Leu Val
465 470 475 480
aag gcc aag act ttg atc gat cca aac aac gtt ttc aac cat cca caa 1488
Lys Ala Lys Thr Leu Ile Asp Pro Asn Asn Val Phe Asn His Pro Gln
485 490 495
tct att cca cca atg atg aag ttt gaa gag atc tac atg ctg aaa gaa 1536
Ser Ile Pro Pro Met Met Lys Phe Glu Glu Ile Tyr Met Leu Lys Glu
500 505 510
ttg tgg tca cat cca caa ttt gaa aag 1563
Leu Trp Ser His Pro Gln Phe Glu Lys
515 520
<210> 51
<211> 521
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 51
Met Gly Asp Val Asn Asp Asn Leu Leu Ser Ser Cys Leu Asn Ser His
1 5 10 15
Gly Val His Asn Phe Thr Thr Leu Ser Thr Asp Thr Asn Ser Asp Tyr
20 25 30
Phe Lys Leu Leu His Ala Ser Met Gln Asn Pro Leu Phe Ala Lys Pro
35 40 45
Thr Val Ser Lys Pro Ser Phe Ile Val Met Pro Gly Ser Lys Glu Glu
50 55 60
Leu Ser Ser Thr Val His Cys Cys Thr Arg Glu Ser Trp Thr Ile Arg
65 70 75 80
Leu Arg Ser Gly Gly His Ser Tyr Glu Gly Leu Ser Tyr Thr Ala Asp
85 90 95
Thr Pro Phe Val Ile Val Asp Met Met Asn Leu Asn Arg Ile Ser Ile
100 105 110
Asp Val Leu Ser Glu Thr Ala Trp Val Glu Ser Gly Ala Thr Leu Gly
115 120 125
Glu Leu Tyr Tyr Ala Ile Ala Gln Ser Thr Asp Thr Leu Gly Phe Thr
130 135 140
Ala Gly Trp Cys Pro Thr Val Gly Ser Gly Gly His Ile Ser Gly Gly
145 150 155 160
Gly Phe Gly Met Met Ser Arg Lys Tyr Gly Leu Ala Ala Asp Asn Val
165 170 175
Val Asp Ala Ile Leu Ile Asp Ser Asn Gly Ala Ile Leu Asp Arg Glu
180 185 190
Lys Met Gly Asp Asp Val Phe Trp Ala Ile Arg Gly Gly Gly Gly Gly
195 200 205
Val Trp Gly Ala Ile Tyr Ala Trp Lys Ile Lys Leu Leu Pro Val Pro
210 215 220
Glu Lys Leu Thr Val Phe Arg Val Thr Lys Asn Val Gly Ile Glu Asp
225 230 235 240
Ala Ser Ser Leu Leu His Lys Trp Gln Tyr Val Ala Asp Glu Leu Asp
245 250 255
Glu Asp Phe Thr Val Ser Val Leu Gly Gly Val Asn Gly Asn Asp Ala
260 265 270
Trp Leu Met Phe Leu Gly Leu His Leu Gly Arg Lys Asp Ala Ala Lys
275 280 285
Thr Ile Ile Asp Glu Lys Phe Pro Glu Leu Gly Leu Val Asp Lys Glu
290 295 300
Phe Gln Glu Met Ser Trp Gly Glu Ser Met Ala Phe Leu Ser Gly Leu
305 310 315 320
Asp Thr Ile Ser Glu Leu Asn Asn Arg Phe Leu Lys Phe Asp Glu Arg
325 330 335
Ala Phe Lys Thr Lys Val Asp Phe Thr Lys Val Ser Val Pro Leu Asn
340 345 350
Val Phe Arg His Ala Leu Glu Met Leu Ser Glu Gln Pro Gly Gly Phe
355 360 365
Ile Ala Leu Asn Gly Phe Gly Gly Lys Met Ser Glu Ile Ser Thr Asp
370 375 380
Phe Thr Pro Phe Pro His Arg Lys Gly Thr Lys Leu Met Phe Glu Tyr
385 390 395 400
Ile Ile Ala Trp Asn Gln Asp Glu Glu Ser Lys Ile Gly Glu Phe Ser
405 410 415
Glu Trp Leu Ala Lys Phe Tyr Asp Tyr Leu Glu Pro Phe Val Ser Lys
420 425 430
Glu Pro Arg Val Gly Tyr Val Asn His Ile Asp Leu Asp Ile Gly Gly
435 440 445
Ile Asp Trp Arg Asn Lys Ser Ser Thr Thr Asn Ala Val Glu Ile Ala
450 455 460
Arg Asn Trp Gly Glu Arg Tyr Phe Ser Ser Asn Tyr Glu Arg Leu Val
465 470 475 480
Lys Ala Lys Thr Leu Ile Asp Pro Asn Asn Val Phe Asn His Pro Gln
485 490 495
Ser Ile Pro Pro Met Met Lys Phe Glu Glu Ile Tyr Met Leu Lys Glu
500 505 510
Leu Trp Ser His Pro Gln Phe Glu Lys
515 520
<210> 52
<211> 1620
<212> DNA
<213> 人工序列
<220>
<223> CBS69-P93479-截断alpha因子-无信号肽-strep标签
<220>
<221> CDS
<222> (1)..(1620)
<400> 52
atg aga ttt cct tcc atc ttc acg gct gtg cta ttt gca gca tcc tcc 48
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser
1 5 10 15
gca ctt gca atg ggt gat gtt aac gat aac ttg ttg tcc tct tgc ttg 96
Ala Leu Ala Met Gly Asp Val Asn Asp Asn Leu Leu Ser Ser Cys Leu
20 25 30
aac tct cat ggt gtt cat aac ttc act act ttg tct acc gat acc aac 144
Asn Ser His Gly Val His Asn Phe Thr Thr Leu Ser Thr Asp Thr Asn
35 40 45
tcc gat tac ttc aag ttg ttg cat gct tct atg caa aac cct ttg ttt 192
Ser Asp Tyr Phe Lys Leu Leu His Ala Ser Met Gln Asn Pro Leu Phe
50 55 60
gct aag cca acc gtt tct aag cca tct ttt atc gtt atg cca ggc tcc 240
Ala Lys Pro Thr Val Ser Lys Pro Ser Phe Ile Val Met Pro Gly Ser
65 70 75 80
aaa gaa gaa ttg tca tct act gtt cat tgc tgc act aga gaa tct tgg 288
Lys Glu Glu Leu Ser Ser Thr Val His Cys Cys Thr Arg Glu Ser Trp
85 90 95
acc att aga ttg aga tct ggt ggt cat tct tac gag ggt ttg tct tat 336
Thr Ile Arg Leu Arg Ser Gly Gly His Ser Tyr Glu Gly Leu Ser Tyr
100 105 110
act gct gat act cca ttc gtt atc gtc gac atg atg aac ttg aac aga 384
Thr Ala Asp Thr Pro Phe Val Ile Val Asp Met Met Asn Leu Asn Arg
115 120 125
atc tcc atc gat gtc ttg tct gaa act gct tgg gtt gaa tct ggt gct 432
Ile Ser Ile Asp Val Leu Ser Glu Thr Ala Trp Val Glu Ser Gly Ala
130 135 140
act ttg ggt gaa ctg tat tat gct att gct caa tcc act gat acc ttg 480
Thr Leu Gly Glu Leu Tyr Tyr Ala Ile Ala Gln Ser Thr Asp Thr Leu
145 150 155 160
ggt ttt act gct ggt tgg tgt cca act gtt ggt tca ggt ggt cat att 528
Gly Phe Thr Ala Gly Trp Cys Pro Thr Val Gly Ser Gly Gly His Ile
165 170 175
tct ggt ggt ggt ttt ggt atg atg tct aga aag tat ggt ttg gct gct 576
Ser Gly Gly Gly Phe Gly Met Met Ser Arg Lys Tyr Gly Leu Ala Ala
180 185 190
gat aac gtt gtt gat gcc att ttg att gat tcc aac ggt gct atc ttg 624
Asp Asn Val Val Asp Ala Ile Leu Ile Asp Ser Asn Gly Ala Ile Leu
195 200 205
gac aga gaa aaa atg ggt gat gat gtt ttc tgg gct att aga ggc ggt 672
Asp Arg Glu Lys Met Gly Asp Asp Val Phe Trp Ala Ile Arg Gly Gly
210 215 220
ggt ggt ggc gtt tgg ggt gca atc tat gct tgg aaa atc aag tta ttg 720
Gly Gly Gly Val Trp Gly Ala Ile Tyr Ala Trp Lys Ile Lys Leu Leu
225 230 235 240
cca gtg cca gaa aag ttg acc gtt ttc aga gtt act aag aac gtc ggt 768
Pro Val Pro Glu Lys Leu Thr Val Phe Arg Val Thr Lys Asn Val Gly
245 250 255
att gaa gat gcc tct tct ttg ttg cac aaa tgg caa tat gtt gcc gat 816
Ile Glu Asp Ala Ser Ser Leu Leu His Lys Trp Gln Tyr Val Ala Asp
260 265 270
gaa ttg gat gag gat ttc acc gtt tct gtt tta ggt ggt gtt aat ggt 864
Glu Leu Asp Glu Asp Phe Thr Val Ser Val Leu Gly Gly Val Asn Gly
275 280 285
aat gat gcc tgg ttg atg ttc ttg ggt tta cat ttg ggt aga aag gat 912
Asn Asp Ala Trp Leu Met Phe Leu Gly Leu His Leu Gly Arg Lys Asp
290 295 300
gct gcc aag acc att att gac gaa aag ttt cca gaa ttg ggc cta gtc 960
Ala Ala Lys Thr Ile Ile Asp Glu Lys Phe Pro Glu Leu Gly Leu Val
305 310 315 320
gac aaa gaa ttt caa gaa atg tct tgg ggt gaa tcg atg gct ttt ttg 1008
Asp Lys Glu Phe Gln Glu Met Ser Trp Gly Glu Ser Met Ala Phe Leu
325 330 335
tct ggt ttg gat acc atc tct gaa ctg aac aac aga ttc ttg aag ttc 1056
Ser Gly Leu Asp Thr Ile Ser Glu Leu Asn Asn Arg Phe Leu Lys Phe
340 345 350
gac gaa aga gct ttc aag acc aag gtt gat ttc acc aaa gtt tcc gtt 1104
Asp Glu Arg Ala Phe Lys Thr Lys Val Asp Phe Thr Lys Val Ser Val
355 360 365
cca ttg aac gtt ttt aga cac gct ttg gaa atg ttg tct gaa caa cca 1152
Pro Leu Asn Val Phe Arg His Ala Leu Glu Met Leu Ser Glu Gln Pro
370 375 380
ggt ggt ttc att gct ttg aat ggt ttc ggt ggt aag atg tcc gaa att 1200
Gly Gly Phe Ile Ala Leu Asn Gly Phe Gly Gly Lys Met Ser Glu Ile
385 390 395 400
tct act gac ttt acc cca ttt cca cac aga aaa ggt acg aag ttg atg 1248
Ser Thr Asp Phe Thr Pro Phe Pro His Arg Lys Gly Thr Lys Leu Met
405 410 415
ttt gag tac att att gcc tgg aac cag gac gaa gaa tct aag att ggt 1296
Phe Glu Tyr Ile Ile Ala Trp Asn Gln Asp Glu Glu Ser Lys Ile Gly
420 425 430
gaa ttt tct gaa tgg ctg gcc aag ttc tat gat tac ttg gaa cca ttc 1344
Glu Phe Ser Glu Trp Leu Ala Lys Phe Tyr Asp Tyr Leu Glu Pro Phe
435 440 445
gtt tcc aaa gaa cct aga gtt ggt tac gtt aac cac atc gat ttg gat 1392
Val Ser Lys Glu Pro Arg Val Gly Tyr Val Asn His Ile Asp Leu Asp
450 455 460
att ggt ggt atc gat tgg agg aac aaa tct tct act acc aac gct gtt 1440
Ile Gly Gly Ile Asp Trp Arg Asn Lys Ser Ser Thr Thr Asn Ala Val
465 470 475 480
gaa att gct aga aac tgg ggt gaa aga tac ttc agc tct aac tac gaa 1488
Glu Ile Ala Arg Asn Trp Gly Glu Arg Tyr Phe Ser Ser Asn Tyr Glu
485 490 495
aga ttg gtt aag gcc aag act ttg atc gat cca aac aac gtt ttc aac 1536
Arg Leu Val Lys Ala Lys Thr Leu Ile Asp Pro Asn Asn Val Phe Asn
500 505 510
cat cca caa tct att cca cca atg atg aag ttt gaa gag atc tac atg 1584
His Pro Gln Ser Ile Pro Pro Met Met Lys Phe Glu Glu Ile Tyr Met
515 520 525
ctg aaa gaa ttg tgg tca cat cca caa ttt gaa aag 1620
Leu Lys Glu Leu Trp Ser His Pro Gln Phe Glu Lys
530 535 540
<210> 53
<211> 540
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 53
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser
1 5 10 15
Ala Leu Ala Met Gly Asp Val Asn Asp Asn Leu Leu Ser Ser Cys Leu
20 25 30
Asn Ser His Gly Val His Asn Phe Thr Thr Leu Ser Thr Asp Thr Asn
35 40 45
Ser Asp Tyr Phe Lys Leu Leu His Ala Ser Met Gln Asn Pro Leu Phe
50 55 60
Ala Lys Pro Thr Val Ser Lys Pro Ser Phe Ile Val Met Pro Gly Ser
65 70 75 80
Lys Glu Glu Leu Ser Ser Thr Val His Cys Cys Thr Arg Glu Ser Trp
85 90 95
Thr Ile Arg Leu Arg Ser Gly Gly His Ser Tyr Glu Gly Leu Ser Tyr
100 105 110
Thr Ala Asp Thr Pro Phe Val Ile Val Asp Met Met Asn Leu Asn Arg
115 120 125
Ile Ser Ile Asp Val Leu Ser Glu Thr Ala Trp Val Glu Ser Gly Ala
130 135 140
Thr Leu Gly Glu Leu Tyr Tyr Ala Ile Ala Gln Ser Thr Asp Thr Leu
145 150 155 160
Gly Phe Thr Ala Gly Trp Cys Pro Thr Val Gly Ser Gly Gly His Ile
165 170 175
Ser Gly Gly Gly Phe Gly Met Met Ser Arg Lys Tyr Gly Leu Ala Ala
180 185 190
Asp Asn Val Val Asp Ala Ile Leu Ile Asp Ser Asn Gly Ala Ile Leu
195 200 205
Asp Arg Glu Lys Met Gly Asp Asp Val Phe Trp Ala Ile Arg Gly Gly
210 215 220
Gly Gly Gly Val Trp Gly Ala Ile Tyr Ala Trp Lys Ile Lys Leu Leu
225 230 235 240
Pro Val Pro Glu Lys Leu Thr Val Phe Arg Val Thr Lys Asn Val Gly
245 250 255
Ile Glu Asp Ala Ser Ser Leu Leu His Lys Trp Gln Tyr Val Ala Asp
260 265 270
Glu Leu Asp Glu Asp Phe Thr Val Ser Val Leu Gly Gly Val Asn Gly
275 280 285
Asn Asp Ala Trp Leu Met Phe Leu Gly Leu His Leu Gly Arg Lys Asp
290 295 300
Ala Ala Lys Thr Ile Ile Asp Glu Lys Phe Pro Glu Leu Gly Leu Val
305 310 315 320
Asp Lys Glu Phe Gln Glu Met Ser Trp Gly Glu Ser Met Ala Phe Leu
325 330 335
Ser Gly Leu Asp Thr Ile Ser Glu Leu Asn Asn Arg Phe Leu Lys Phe
340 345 350
Asp Glu Arg Ala Phe Lys Thr Lys Val Asp Phe Thr Lys Val Ser Val
355 360 365
Pro Leu Asn Val Phe Arg His Ala Leu Glu Met Leu Ser Glu Gln Pro
370 375 380
Gly Gly Phe Ile Ala Leu Asn Gly Phe Gly Gly Lys Met Ser Glu Ile
385 390 395 400
Ser Thr Asp Phe Thr Pro Phe Pro His Arg Lys Gly Thr Lys Leu Met
405 410 415
Phe Glu Tyr Ile Ile Ala Trp Asn Gln Asp Glu Glu Ser Lys Ile Gly
420 425 430
Glu Phe Ser Glu Trp Leu Ala Lys Phe Tyr Asp Tyr Leu Glu Pro Phe
435 440 445
Val Ser Lys Glu Pro Arg Val Gly Tyr Val Asn His Ile Asp Leu Asp
450 455 460
Ile Gly Gly Ile Asp Trp Arg Asn Lys Ser Ser Thr Thr Asn Ala Val
465 470 475 480
Glu Ile Ala Arg Asn Trp Gly Glu Arg Tyr Phe Ser Ser Asn Tyr Glu
485 490 495
Arg Leu Val Lys Ala Lys Thr Leu Ile Asp Pro Asn Asn Val Phe Asn
500 505 510
His Pro Gln Ser Ile Pro Pro Met Met Lys Phe Glu Glu Ile Tyr Met
515 520 525
Leu Lys Glu Leu Trp Ser His Pro Gln Phe Glu Lys
530 535 540
<210> 54
<211> 1830
<212> DNA
<213> 人工序列
<220>
<223> CBS69-P93479-全长alpha因子-无信号肽-strep标签
<220>
<221> CDS
<222> (1)..(1830)
<400> 54
atg aga ttt cct tca att ttt act gct gtt tta ttc gca gca tcc tcc 48
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser
1 5 10 15
gca tta gct gct cca gtc aac act aca aca gaa gat gaa acg gca caa 96
Ala Leu Ala Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln
20 25 30
att ccg gct gaa gct gtc atc ggt tac tca gat tta gaa ggg gat ttc 144
Ile Pro Ala Glu Ala Val Ile Gly Tyr Ser Asp Leu Glu Gly Asp Phe
35 40 45
gat gtt gct gtt ttg cca ttt tcc aac agc aca aat aac ggg tta ttg 192
Asp Val Ala Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu
50 55 60
ttt ata aat act act att gcc agc att gct gct aaa gaa gaa ggg gta 240
Phe Ile Asn Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val
65 70 75 80
tct ctc gag aaa aga gag gct gaa gct atg ggt gat gtt aac gat aac 288
Ser Leu Glu Lys Arg Glu Ala Glu Ala Met Gly Asp Val Asn Asp Asn
85 90 95
ttg ttg tcc tct tgc ttg aac tct cat ggt gtt cat aac ttc act act 336
Leu Leu Ser Ser Cys Leu Asn Ser His Gly Val His Asn Phe Thr Thr
100 105 110
ttg tct acc gat acc aac tcc gat tac ttc aag ttg ttg cat gct tct 384
Leu Ser Thr Asp Thr Asn Ser Asp Tyr Phe Lys Leu Leu His Ala Ser
115 120 125
atg caa aac cct ttg ttt gct aag cca acc gtt tct aag cca tct ttt 432
Met Gln Asn Pro Leu Phe Ala Lys Pro Thr Val Ser Lys Pro Ser Phe
130 135 140
atc gtt atg cca ggc tcc aaa gaa gaa ttg tca tct act gtt cat tgc 480
Ile Val Met Pro Gly Ser Lys Glu Glu Leu Ser Ser Thr Val His Cys
145 150 155 160
tgc act aga gaa tct tgg acc att aga ttg aga tct ggt ggt cat tct 528
Cys Thr Arg Glu Ser Trp Thr Ile Arg Leu Arg Ser Gly Gly His Ser
165 170 175
tac gag ggt ttg tct tat act gct gat act cca ttc gtt atc gtc gac 576
Tyr Glu Gly Leu Ser Tyr Thr Ala Asp Thr Pro Phe Val Ile Val Asp
180 185 190
atg atg aac ttg aac aga atc tcc atc gat gtc ttg tct gaa act gct 624
Met Met Asn Leu Asn Arg Ile Ser Ile Asp Val Leu Ser Glu Thr Ala
195 200 205
tgg gtt gaa tct ggt gct act ttg ggt gaa ctg tat tat gct att gct 672
Trp Val Glu Ser Gly Ala Thr Leu Gly Glu Leu Tyr Tyr Ala Ile Ala
210 215 220
caa tcc act gat acc ttg ggt ttt act gct ggt tgg tgt cca act gtt 720
Gln Ser Thr Asp Thr Leu Gly Phe Thr Ala Gly Trp Cys Pro Thr Val
225 230 235 240
ggt tca ggt ggt cat att tct ggt ggt ggt ttt ggt atg atg tct aga 768
Gly Ser Gly Gly His Ile Ser Gly Gly Gly Phe Gly Met Met Ser Arg
245 250 255
aag tat ggt ttg gct gct gat aac gtt gtt gat gcc att ttg att gat 816
Lys Tyr Gly Leu Ala Ala Asp Asn Val Val Asp Ala Ile Leu Ile Asp
260 265 270
tcc aac ggt gct atc ttg gac aga gaa aaa atg ggt gat gat gtt ttc 864
Ser Asn Gly Ala Ile Leu Asp Arg Glu Lys Met Gly Asp Asp Val Phe
275 280 285
tgg gct att aga ggc ggt ggt ggt ggc gtt tgg ggt gca atc tat gct 912
Trp Ala Ile Arg Gly Gly Gly Gly Gly Val Trp Gly Ala Ile Tyr Ala
290 295 300
tgg aaa atc aag tta ttg cca gtg cca gaa aag ttg acc gtt ttc aga 960
Trp Lys Ile Lys Leu Leu Pro Val Pro Glu Lys Leu Thr Val Phe Arg
305 310 315 320
gtt act aag aac gtc ggt att gaa gat gcc tct tct ttg ttg cac aaa 1008
Val Thr Lys Asn Val Gly Ile Glu Asp Ala Ser Ser Leu Leu His Lys
325 330 335
tgg caa tat gtt gcc gat gaa ttg gat gag gat ttc acc gtt tct gtt 1056
Trp Gln Tyr Val Ala Asp Glu Leu Asp Glu Asp Phe Thr Val Ser Val
340 345 350
tta ggt ggt gtt aat ggt aat gat gcc tgg ttg atg ttc ttg ggt tta 1104
Leu Gly Gly Val Asn Gly Asn Asp Ala Trp Leu Met Phe Leu Gly Leu
355 360 365
cat ttg ggt aga aag gat gct gcc aag acc att att gac gaa aag ttt 1152
His Leu Gly Arg Lys Asp Ala Ala Lys Thr Ile Ile Asp Glu Lys Phe
370 375 380
cca gaa ttg ggc cta gtc gac aaa gaa ttt caa gaa atg tct tgg ggt 1200
Pro Glu Leu Gly Leu Val Asp Lys Glu Phe Gln Glu Met Ser Trp Gly
385 390 395 400
gaa tcg atg gct ttt ttg tct ggt ttg gat acc atc tct gaa ctg aac 1248
Glu Ser Met Ala Phe Leu Ser Gly Leu Asp Thr Ile Ser Glu Leu Asn
405 410 415
aac aga ttc ttg aag ttc gac gaa aga gct ttc aag acc aag gtt gat 1296
Asn Arg Phe Leu Lys Phe Asp Glu Arg Ala Phe Lys Thr Lys Val Asp
420 425 430
ttc acc aaa gtt tcc gtt cca ttg aac gtt ttt aga cac gct ttg gaa 1344
Phe Thr Lys Val Ser Val Pro Leu Asn Val Phe Arg His Ala Leu Glu
435 440 445
atg ttg tct gaa caa cca ggt ggt ttc att gct ttg aat ggt ttc ggt 1392
Met Leu Ser Glu Gln Pro Gly Gly Phe Ile Ala Leu Asn Gly Phe Gly
450 455 460
ggt aag atg tcc gaa att tct act gac ttt acc cca ttt cca cac aga 1440
Gly Lys Met Ser Glu Ile Ser Thr Asp Phe Thr Pro Phe Pro His Arg
465 470 475 480
aaa ggt acg aag ttg atg ttt gag tac att att gcc tgg aac cag gac 1488
Lys Gly Thr Lys Leu Met Phe Glu Tyr Ile Ile Ala Trp Asn Gln Asp
485 490 495
gaa gaa tct aag att ggt gaa ttt tct gaa tgg ctg gcc aag ttc tat 1536
Glu Glu Ser Lys Ile Gly Glu Phe Ser Glu Trp Leu Ala Lys Phe Tyr
500 505 510
gat tac ttg gaa cca ttc gtt tcc aaa gaa cct aga gtt ggt tac gtt 1584
Asp Tyr Leu Glu Pro Phe Val Ser Lys Glu Pro Arg Val Gly Tyr Val
515 520 525
aac cac atc gat ttg gat att ggt ggt atc gat tgg agg aac aaa tct 1632
Asn His Ile Asp Leu Asp Ile Gly Gly Ile Asp Trp Arg Asn Lys Ser
530 535 540
tct act acc aac gct gtt gaa att gct aga aac tgg ggt gaa aga tac 1680
Ser Thr Thr Asn Ala Val Glu Ile Ala Arg Asn Trp Gly Glu Arg Tyr
545 550 555 560
ttc agc tct aac tac gaa aga ttg gtt aag gcc aag act ttg atc gat 1728
Phe Ser Ser Asn Tyr Glu Arg Leu Val Lys Ala Lys Thr Leu Ile Asp
565 570 575
cca aac aac gtt ttc aac cat cca caa tct att cca cca atg atg aag 1776
Pro Asn Asn Val Phe Asn His Pro Gln Ser Ile Pro Pro Met Met Lys
580 585 590
ttt gaa gag atc tac atg ctg aaa gaa ttg tgg tca cat cca caa ttt 1824
Phe Glu Glu Ile Tyr Met Leu Lys Glu Leu Trp Ser His Pro Gln Phe
595 600 605
gaa aag 1830
Glu Lys
610
<210> 55
<211> 610
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 55
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser
1 5 10 15
Ala Leu Ala Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln
20 25 30
Ile Pro Ala Glu Ala Val Ile Gly Tyr Ser Asp Leu Glu Gly Asp Phe
35 40 45
Asp Val Ala Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu
50 55 60
Phe Ile Asn Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val
65 70 75 80
Ser Leu Glu Lys Arg Glu Ala Glu Ala Met Gly Asp Val Asn Asp Asn
85 90 95
Leu Leu Ser Ser Cys Leu Asn Ser His Gly Val His Asn Phe Thr Thr
100 105 110
Leu Ser Thr Asp Thr Asn Ser Asp Tyr Phe Lys Leu Leu His Ala Ser
115 120 125
Met Gln Asn Pro Leu Phe Ala Lys Pro Thr Val Ser Lys Pro Ser Phe
130 135 140
Ile Val Met Pro Gly Ser Lys Glu Glu Leu Ser Ser Thr Val His Cys
145 150 155 160
Cys Thr Arg Glu Ser Trp Thr Ile Arg Leu Arg Ser Gly Gly His Ser
165 170 175
Tyr Glu Gly Leu Ser Tyr Thr Ala Asp Thr Pro Phe Val Ile Val Asp
180 185 190
Met Met Asn Leu Asn Arg Ile Ser Ile Asp Val Leu Ser Glu Thr Ala
195 200 205
Trp Val Glu Ser Gly Ala Thr Leu Gly Glu Leu Tyr Tyr Ala Ile Ala
210 215 220
Gln Ser Thr Asp Thr Leu Gly Phe Thr Ala Gly Trp Cys Pro Thr Val
225 230 235 240
Gly Ser Gly Gly His Ile Ser Gly Gly Gly Phe Gly Met Met Ser Arg
245 250 255
Lys Tyr Gly Leu Ala Ala Asp Asn Val Val Asp Ala Ile Leu Ile Asp
260 265 270
Ser Asn Gly Ala Ile Leu Asp Arg Glu Lys Met Gly Asp Asp Val Phe
275 280 285
Trp Ala Ile Arg Gly Gly Gly Gly Gly Val Trp Gly Ala Ile Tyr Ala
290 295 300
Trp Lys Ile Lys Leu Leu Pro Val Pro Glu Lys Leu Thr Val Phe Arg
305 310 315 320
Val Thr Lys Asn Val Gly Ile Glu Asp Ala Ser Ser Leu Leu His Lys
325 330 335
Trp Gln Tyr Val Ala Asp Glu Leu Asp Glu Asp Phe Thr Val Ser Val
340 345 350
Leu Gly Gly Val Asn Gly Asn Asp Ala Trp Leu Met Phe Leu Gly Leu
355 360 365
His Leu Gly Arg Lys Asp Ala Ala Lys Thr Ile Ile Asp Glu Lys Phe
370 375 380
Pro Glu Leu Gly Leu Val Asp Lys Glu Phe Gln Glu Met Ser Trp Gly
385 390 395 400
Glu Ser Met Ala Phe Leu Ser Gly Leu Asp Thr Ile Ser Glu Leu Asn
405 410 415
Asn Arg Phe Leu Lys Phe Asp Glu Arg Ala Phe Lys Thr Lys Val Asp
420 425 430
Phe Thr Lys Val Ser Val Pro Leu Asn Val Phe Arg His Ala Leu Glu
435 440 445
Met Leu Ser Glu Gln Pro Gly Gly Phe Ile Ala Leu Asn Gly Phe Gly
450 455 460
Gly Lys Met Ser Glu Ile Ser Thr Asp Phe Thr Pro Phe Pro His Arg
465 470 475 480
Lys Gly Thr Lys Leu Met Phe Glu Tyr Ile Ile Ala Trp Asn Gln Asp
485 490 495
Glu Glu Ser Lys Ile Gly Glu Phe Ser Glu Trp Leu Ala Lys Phe Tyr
500 505 510
Asp Tyr Leu Glu Pro Phe Val Ser Lys Glu Pro Arg Val Gly Tyr Val
515 520 525
Asn His Ile Asp Leu Asp Ile Gly Gly Ile Asp Trp Arg Asn Lys Ser
530 535 540
Ser Thr Thr Asn Ala Val Glu Ile Ala Arg Asn Trp Gly Glu Arg Tyr
545 550 555 560
Phe Ser Ser Asn Tyr Glu Arg Leu Val Lys Ala Lys Thr Leu Ile Asp
565 570 575
Pro Asn Asn Val Phe Asn His Pro Gln Ser Ile Pro Pro Met Met Lys
580 585 590
Phe Glu Glu Ile Tyr Met Leu Lys Glu Leu Trp Ser His Pro Gln Phe
595 600 605
Glu Lys
610
<210> 56
<211> 1611
<212> DNA
<213> 人工序列
<220>
<223> CBS86-A0A1U8G0D2
<220>
<221> CDS
<222> (1)..(1611)
<400> 56
atg atg ggt tcc ttg caa atc ttg ttc gtc ttg ttg ttc tcc ctg gtt 48
Met Met Gly Ser Leu Gln Ile Leu Phe Val Leu Leu Phe Ser Leu Val
1 5 10 15
ttg att aag tgc tac tct caa gag gat ttc ttg gag tgt ttg tcc aag 96
Leu Ile Lys Cys Tyr Ser Gln Glu Asp Phe Leu Glu Cys Leu Ser Lys
20 25 30
tac tct aag tcc aac gtt gaa caa aac gtt tac act cca act tct cca 144
Tyr Ser Lys Ser Asn Val Glu Gln Asn Val Tyr Thr Pro Thr Ser Pro
35 40 45
acc tac tcc tct att ttg gaa tac gct caa aag aac cca aga tgg gtt 192
Thr Tyr Ser Ser Ile Leu Glu Tyr Ala Gln Lys Asn Pro Arg Trp Val
50 55 60
aat tct tct cac cca aac ttt att gcc tcg cca aga aaa gaa tct gac 240
Asn Ser Ser His Pro Asn Phe Ile Ala Ser Pro Arg Lys Glu Ser Asp
65 70 75 80
atc aag cca att atc ttg tgc gct aag aaa ttg ggc ttg cag att aag 288
Ile Lys Pro Ile Ile Leu Cys Ala Lys Lys Leu Gly Leu Gln Ile Lys
85 90 95
att aag tcc ggt ggt cat gat tac gag ggt att tct tac aga tct aag 336
Ile Lys Ser Gly Gly His Asp Tyr Glu Gly Ile Ser Tyr Arg Ser Lys
100 105 110
tcc cca ttc gtt atg ttg gac ttg tct aac ttg aac aag atc gag atc 384
Ser Pro Phe Val Met Leu Asp Leu Ser Asn Leu Asn Lys Ile Glu Ile
115 120 125
aac tgg aag aaa gaa acc gtt tgg gtt caa act ggt gct act ttg ggt 432
Asn Trp Lys Lys Glu Thr Val Trp Val Gln Thr Gly Ala Thr Leu Gly
130 135 140
caa ctg tat tat gct att gcc aac aag tct aac gtt cat ggt ttt cca 480
Gln Leu Tyr Tyr Ala Ile Ala Asn Lys Ser Asn Val His Gly Phe Pro
145 150 155 160
ggt ggt gtt tgt ttc tct att ggt act ggt ggt att att tct ggt ggt 528
Gly Gly Val Cys Phe Ser Ile Gly Thr Gly Gly Ile Ile Ser Gly Gly
165 170 175
ggt ata ggt act atg atg aga aaa ttt ggt gtt gcc gct gat aac gtt 576
Gly Ile Gly Thr Met Met Arg Lys Phe Gly Val Ala Ala Asp Asn Val
180 185 190
gtt gat gct aga gtt atg gat gtc aac ggt aag atc ttg gac aag aaa 624
Val Asp Ala Arg Val Met Asp Val Asn Gly Lys Ile Leu Asp Lys Lys
195 200 205
aag atg aag gaa gat ttg ttc tgg gcc att aga ggt ggt ggt ggc gct 672
Lys Met Lys Glu Asp Leu Phe Trp Ala Ile Arg Gly Gly Gly Gly Ala
210 215 220
tct ttt ggt gtt att ttg gct tgg aaa ttg aag ttg gtt agg gtt cca 720
Ser Phe Gly Val Ile Leu Ala Trp Lys Leu Lys Leu Val Arg Val Pro
225 230 235 240
gaa aag gtc act gtt ttt aac gtc tac aaa aag ttg gaa ggc aac cag 768
Glu Lys Val Thr Val Phe Asn Val Tyr Lys Lys Leu Glu Gly Asn Gln
245 250 255
aac ttg ttg caa aag tgg gaa aac att gct cat caa ttg acc gac aag 816
Asn Leu Leu Gln Lys Trp Glu Asn Ile Ala His Gln Leu Thr Asp Lys
260 265 270
ttg ttg atc aga gtc atc att caa aac gac ggt act ggt aag gat aag 864
Leu Leu Ile Arg Val Ile Ile Gln Asn Asp Gly Thr Gly Lys Asp Lys
275 280 285
tac atc gaa gtt atc ttc caa gcc ttg ttc ttg ggt tta gtt gat gaa 912
Tyr Ile Glu Val Ile Phe Gln Ala Leu Phe Leu Gly Leu Val Asp Glu
290 295 300
ttg atc ccc ttg ctg aaa gaa aag ttc cca gaa ttc gat ttg gaa aag 960
Leu Ile Pro Leu Leu Lys Glu Lys Phe Pro Glu Phe Asp Leu Glu Lys
305 310 315 320
aag gac tgt ttc caa gaa cca gtt gtt gac tgt tct aac aga cca tgc 1008
Lys Asp Cys Phe Gln Glu Pro Val Val Asp Cys Ser Asn Arg Pro Cys
325 330 335
att aag aaa gaa tgc aga gaa act acc tgg atc ggt tct gtt ttg ttt 1056
Ile Lys Lys Glu Cys Arg Glu Thr Thr Trp Ile Gly Ser Val Leu Phe
340 345 350
ttc tac ggt aga agg acc aac gaa tcc ttg gaa gtt ttg ttg gat aag 1104
Phe Tyr Gly Arg Arg Thr Asn Glu Ser Leu Glu Val Leu Leu Asp Lys
355 360 365
tcc att cca acg cag aag aac tac aac aaa ggt act tct gat ttc gtc 1152
Ser Ile Pro Thr Gln Lys Asn Tyr Asn Lys Gly Thr Ser Asp Phe Val
370 375 380
aag acc cca att cca gaa tct ggt tgg aaa atg gtt caa agg ttg ttc 1200
Lys Thr Pro Ile Pro Glu Ser Gly Trp Lys Met Val Gln Arg Leu Phe
385 390 395 400
tta gaa gag gaa cgt cca caa atg atc ttg gaa cca tta ggt ggt aag 1248
Leu Glu Glu Glu Arg Pro Gln Met Ile Leu Glu Pro Leu Gly Gly Lys
405 410 415
ttg gac gaa atc tcc aaa tcc gaa att tca ttc cca cac aga aag ggc 1296
Leu Asp Glu Ile Ser Lys Ser Glu Ile Ser Phe Pro His Arg Lys Gly
420 425 430
aac ttg tac aac att caa tac ttg gtt aac tgg ggt gac aac tcc gaa 1344
Asn Leu Tyr Asn Ile Gln Tyr Leu Val Asn Trp Gly Asp Asn Ser Glu
435 440 445
aat gtt tcc tct caa aaa ttg gcc tgg atg cgt aag ttg tac aaa gat 1392
Asn Val Ser Ser Gln Lys Leu Ala Trp Met Arg Lys Leu Tyr Lys Asp
450 455 460
atg gaa cca tac gtt gcc aac tct cca aga act gct tat ttg aac tac 1440
Met Glu Pro Tyr Val Ala Asn Ser Pro Arg Thr Ala Tyr Leu Asn Tyr
465 470 475 480
aga gat ttg gac ttc ggt tcc aat gat gaa ggt tac tct tac tcc aaa 1488
Arg Asp Leu Asp Phe Gly Ser Asn Asp Glu Gly Tyr Ser Tyr Ser Lys
485 490 495
gct aag att tgg ggt gaa aag tac ttc aac ggc aac ttt aag aga ttg 1536
Ala Lys Ile Trp Gly Glu Lys Tyr Phe Asn Gly Asn Phe Lys Arg Leu
500 505 510
gcc aag gtt aag tct aag gtc gat cca aac aat ttc ttc aga aac gaa 1584
Ala Lys Val Lys Ser Lys Val Asp Pro Asn Asn Phe Phe Arg Asn Glu
515 520 525
caa tcg atc cca cca tat caa tcc cat 1611
Gln Ser Ile Pro Pro Tyr Gln Ser His
530 535
<210> 57
<211> 537
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 57
Met Met Gly Ser Leu Gln Ile Leu Phe Val Leu Leu Phe Ser Leu Val
1 5 10 15
Leu Ile Lys Cys Tyr Ser Gln Glu Asp Phe Leu Glu Cys Leu Ser Lys
20 25 30
Tyr Ser Lys Ser Asn Val Glu Gln Asn Val Tyr Thr Pro Thr Ser Pro
35 40 45
Thr Tyr Ser Ser Ile Leu Glu Tyr Ala Gln Lys Asn Pro Arg Trp Val
50 55 60
Asn Ser Ser His Pro Asn Phe Ile Ala Ser Pro Arg Lys Glu Ser Asp
65 70 75 80
Ile Lys Pro Ile Ile Leu Cys Ala Lys Lys Leu Gly Leu Gln Ile Lys
85 90 95
Ile Lys Ser Gly Gly His Asp Tyr Glu Gly Ile Ser Tyr Arg Ser Lys
100 105 110
Ser Pro Phe Val Met Leu Asp Leu Ser Asn Leu Asn Lys Ile Glu Ile
115 120 125
Asn Trp Lys Lys Glu Thr Val Trp Val Gln Thr Gly Ala Thr Leu Gly
130 135 140
Gln Leu Tyr Tyr Ala Ile Ala Asn Lys Ser Asn Val His Gly Phe Pro
145 150 155 160
Gly Gly Val Cys Phe Ser Ile Gly Thr Gly Gly Ile Ile Ser Gly Gly
165 170 175
Gly Ile Gly Thr Met Met Arg Lys Phe Gly Val Ala Ala Asp Asn Val
180 185 190
Val Asp Ala Arg Val Met Asp Val Asn Gly Lys Ile Leu Asp Lys Lys
195 200 205
Lys Met Lys Glu Asp Leu Phe Trp Ala Ile Arg Gly Gly Gly Gly Ala
210 215 220
Ser Phe Gly Val Ile Leu Ala Trp Lys Leu Lys Leu Val Arg Val Pro
225 230 235 240
Glu Lys Val Thr Val Phe Asn Val Tyr Lys Lys Leu Glu Gly Asn Gln
245 250 255
Asn Leu Leu Gln Lys Trp Glu Asn Ile Ala His Gln Leu Thr Asp Lys
260 265 270
Leu Leu Ile Arg Val Ile Ile Gln Asn Asp Gly Thr Gly Lys Asp Lys
275 280 285
Tyr Ile Glu Val Ile Phe Gln Ala Leu Phe Leu Gly Leu Val Asp Glu
290 295 300
Leu Ile Pro Leu Leu Lys Glu Lys Phe Pro Glu Phe Asp Leu Glu Lys
305 310 315 320
Lys Asp Cys Phe Gln Glu Pro Val Val Asp Cys Ser Asn Arg Pro Cys
325 330 335
Ile Lys Lys Glu Cys Arg Glu Thr Thr Trp Ile Gly Ser Val Leu Phe
340 345 350
Phe Tyr Gly Arg Arg Thr Asn Glu Ser Leu Glu Val Leu Leu Asp Lys
355 360 365
Ser Ile Pro Thr Gln Lys Asn Tyr Asn Lys Gly Thr Ser Asp Phe Val
370 375 380
Lys Thr Pro Ile Pro Glu Ser Gly Trp Lys Met Val Gln Arg Leu Phe
385 390 395 400
Leu Glu Glu Glu Arg Pro Gln Met Ile Leu Glu Pro Leu Gly Gly Lys
405 410 415
Leu Asp Glu Ile Ser Lys Ser Glu Ile Ser Phe Pro His Arg Lys Gly
420 425 430
Asn Leu Tyr Asn Ile Gln Tyr Leu Val Asn Trp Gly Asp Asn Ser Glu
435 440 445
Asn Val Ser Ser Gln Lys Leu Ala Trp Met Arg Lys Leu Tyr Lys Asp
450 455 460
Met Glu Pro Tyr Val Ala Asn Ser Pro Arg Thr Ala Tyr Leu Asn Tyr
465 470 475 480
Arg Asp Leu Asp Phe Gly Ser Asn Asp Glu Gly Tyr Ser Tyr Ser Lys
485 490 495
Ala Lys Ile Trp Gly Glu Lys Tyr Phe Asn Gly Asn Phe Lys Arg Leu
500 505 510
Ala Lys Val Lys Ser Lys Val Asp Pro Asn Asn Phe Phe Arg Asn Glu
515 520 525
Gln Ser Ile Pro Pro Tyr Gln Ser His
530 535
<210> 58
<211> 1551
<212> DNA
<213> 人工序列
<220>
<223> CBS86-A0A1U8G0D2-无信号肽
<220>
<221> CDS
<222> (1)..(1551)
<400> 58
atg tct caa gag gat ttc ttg gag tgt ttg tcc aag tac tct aag tcc 48
Met Ser Gln Glu Asp Phe Leu Glu Cys Leu Ser Lys Tyr Ser Lys Ser
1 5 10 15
aac gtt gaa caa aac gtt tac act cca act tct cca acc tac tcc tct 96
Asn Val Glu Gln Asn Val Tyr Thr Pro Thr Ser Pro Thr Tyr Ser Ser
20 25 30
att ttg gaa tac gct caa aag aac cca aga tgg gtt aat tct tct cac 144
Ile Leu Glu Tyr Ala Gln Lys Asn Pro Arg Trp Val Asn Ser Ser His
35 40 45
cca aac ttt att gcc tcg cca aga aaa gaa tct gac atc aag cca att 192
Pro Asn Phe Ile Ala Ser Pro Arg Lys Glu Ser Asp Ile Lys Pro Ile
50 55 60
atc ttg tgc gct aag aaa ttg ggc ttg cag att aag att aag tcc ggt 240
Ile Leu Cys Ala Lys Lys Leu Gly Leu Gln Ile Lys Ile Lys Ser Gly
65 70 75 80
ggt cat gat tac gag ggt att tct tac aga tct aag tcc cca ttc gtt 288
Gly His Asp Tyr Glu Gly Ile Ser Tyr Arg Ser Lys Ser Pro Phe Val
85 90 95
atg ttg gac ttg tct aac ttg aac aag atc gag atc aac tgg aag aaa 336
Met Leu Asp Leu Ser Asn Leu Asn Lys Ile Glu Ile Asn Trp Lys Lys
100 105 110
gaa acc gtt tgg gtt caa act ggt gct act ttg ggt caa ctg tat tat 384
Glu Thr Val Trp Val Gln Thr Gly Ala Thr Leu Gly Gln Leu Tyr Tyr
115 120 125
gct att gcc aac aag tct aac gtt cat ggt ttt cca ggt ggt gtt tgt 432
Ala Ile Ala Asn Lys Ser Asn Val His Gly Phe Pro Gly Gly Val Cys
130 135 140
ttc tct att ggt act ggt ggt att att tct ggt ggt ggt ata ggt act 480
Phe Ser Ile Gly Thr Gly Gly Ile Ile Ser Gly Gly Gly Ile Gly Thr
145 150 155 160
atg atg aga aaa ttt ggt gtt gcc gct gat aac gtt gtt gat gct aga 528
Met Met Arg Lys Phe Gly Val Ala Ala Asp Asn Val Val Asp Ala Arg
165 170 175
gtt atg gat gtc aac ggt aag atc ttg gac aag aaa aag atg aag gaa 576
Val Met Asp Val Asn Gly Lys Ile Leu Asp Lys Lys Lys Met Lys Glu
180 185 190
gat ttg ttc tgg gcc att aga ggt ggt ggt ggc gct tct ttt ggt gtt 624
Asp Leu Phe Trp Ala Ile Arg Gly Gly Gly Gly Ala Ser Phe Gly Val
195 200 205
att ttg gct tgg aaa ttg aag ttg gtt agg gtt cca gaa aag gtc act 672
Ile Leu Ala Trp Lys Leu Lys Leu Val Arg Val Pro Glu Lys Val Thr
210 215 220
gtt ttt aac gtc tac aaa aag ttg gaa ggc aac cag aac ttg ttg caa 720
Val Phe Asn Val Tyr Lys Lys Leu Glu Gly Asn Gln Asn Leu Leu Gln
225 230 235 240
aag tgg gaa aac att gct cat caa ttg acc gac aag ttg ttg atc aga 768
Lys Trp Glu Asn Ile Ala His Gln Leu Thr Asp Lys Leu Leu Ile Arg
245 250 255
gtc atc att caa aac gac ggt act ggt aag gat aag tac atc gaa gtt 816
Val Ile Ile Gln Asn Asp Gly Thr Gly Lys Asp Lys Tyr Ile Glu Val
260 265 270
atc ttc caa gcc ttg ttc ttg ggt tta gtt gat gaa ttg atc ccc ttg 864
Ile Phe Gln Ala Leu Phe Leu Gly Leu Val Asp Glu Leu Ile Pro Leu
275 280 285
ctg aaa gaa aag ttc cca gaa ttc gat ttg gaa aag aag gac tgt ttc 912
Leu Lys Glu Lys Phe Pro Glu Phe Asp Leu Glu Lys Lys Asp Cys Phe
290 295 300
caa gaa cca gtt gtt gac tgt tct aac aga cca tgc att aag aaa gaa 960
Gln Glu Pro Val Val Asp Cys Ser Asn Arg Pro Cys Ile Lys Lys Glu
305 310 315 320
tgc aga gaa act acc tgg atc ggt tct gtt ttg ttt ttc tac ggt aga 1008
Cys Arg Glu Thr Thr Trp Ile Gly Ser Val Leu Phe Phe Tyr Gly Arg
325 330 335
agg acc aac gaa tcc ttg gaa gtt ttg ttg gat aag tcc att cca acg 1056
Arg Thr Asn Glu Ser Leu Glu Val Leu Leu Asp Lys Ser Ile Pro Thr
340 345 350
cag aag aac tac aac aaa ggt act tct gat ttc gtc aag acc cca att 1104
Gln Lys Asn Tyr Asn Lys Gly Thr Ser Asp Phe Val Lys Thr Pro Ile
355 360 365
cca gaa tct ggt tgg aaa atg gtt caa agg ttg ttc tta gaa gag gaa 1152
Pro Glu Ser Gly Trp Lys Met Val Gln Arg Leu Phe Leu Glu Glu Glu
370 375 380
cgt cca caa atg atc ttg gaa cca tta ggt ggt aag ttg gac gaa atc 1200
Arg Pro Gln Met Ile Leu Glu Pro Leu Gly Gly Lys Leu Asp Glu Ile
385 390 395 400
tcc aaa tcc gaa att tca ttc cca cac aga aag ggc aac ttg tac aac 1248
Ser Lys Ser Glu Ile Ser Phe Pro His Arg Lys Gly Asn Leu Tyr Asn
405 410 415
att caa tac ttg gtt aac tgg ggt gac aac tcc gaa aat gtt tcc tct 1296
Ile Gln Tyr Leu Val Asn Trp Gly Asp Asn Ser Glu Asn Val Ser Ser
420 425 430
caa aaa ttg gcc tgg atg cgt aag ttg tac aaa gat atg gaa cca tac 1344
Gln Lys Leu Ala Trp Met Arg Lys Leu Tyr Lys Asp Met Glu Pro Tyr
435 440 445
gtt gcc aac tct cca aga act gct tat ttg aac tac aga gat ttg gac 1392
Val Ala Asn Ser Pro Arg Thr Ala Tyr Leu Asn Tyr Arg Asp Leu Asp
450 455 460
ttc ggt tcc aat gat gaa ggt tac tct tac tcc aaa gct aag att tgg 1440
Phe Gly Ser Asn Asp Glu Gly Tyr Ser Tyr Ser Lys Ala Lys Ile Trp
465 470 475 480
ggt gaa aag tac ttc aac ggc aac ttt aag aga ttg gcc aag gtt aag 1488
Gly Glu Lys Tyr Phe Asn Gly Asn Phe Lys Arg Leu Ala Lys Val Lys
485 490 495
tct aag gtc gat cca aac aat ttc ttc aga aac gaa caa tcg atc cca 1536
Ser Lys Val Asp Pro Asn Asn Phe Phe Arg Asn Glu Gln Ser Ile Pro
500 505 510
cca tat caa tcc cat 1551
Pro Tyr Gln Ser His
515
<210> 59
<211> 517
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 59
Met Ser Gln Glu Asp Phe Leu Glu Cys Leu Ser Lys Tyr Ser Lys Ser
1 5 10 15
Asn Val Glu Gln Asn Val Tyr Thr Pro Thr Ser Pro Thr Tyr Ser Ser
20 25 30
Ile Leu Glu Tyr Ala Gln Lys Asn Pro Arg Trp Val Asn Ser Ser His
35 40 45
Pro Asn Phe Ile Ala Ser Pro Arg Lys Glu Ser Asp Ile Lys Pro Ile
50 55 60
Ile Leu Cys Ala Lys Lys Leu Gly Leu Gln Ile Lys Ile Lys Ser Gly
65 70 75 80
Gly His Asp Tyr Glu Gly Ile Ser Tyr Arg Ser Lys Ser Pro Phe Val
85 90 95
Met Leu Asp Leu Ser Asn Leu Asn Lys Ile Glu Ile Asn Trp Lys Lys
100 105 110
Glu Thr Val Trp Val Gln Thr Gly Ala Thr Leu Gly Gln Leu Tyr Tyr
115 120 125
Ala Ile Ala Asn Lys Ser Asn Val His Gly Phe Pro Gly Gly Val Cys
130 135 140
Phe Ser Ile Gly Thr Gly Gly Ile Ile Ser Gly Gly Gly Ile Gly Thr
145 150 155 160
Met Met Arg Lys Phe Gly Val Ala Ala Asp Asn Val Val Asp Ala Arg
165 170 175
Val Met Asp Val Asn Gly Lys Ile Leu Asp Lys Lys Lys Met Lys Glu
180 185 190
Asp Leu Phe Trp Ala Ile Arg Gly Gly Gly Gly Ala Ser Phe Gly Val
195 200 205
Ile Leu Ala Trp Lys Leu Lys Leu Val Arg Val Pro Glu Lys Val Thr
210 215 220
Val Phe Asn Val Tyr Lys Lys Leu Glu Gly Asn Gln Asn Leu Leu Gln
225 230 235 240
Lys Trp Glu Asn Ile Ala His Gln Leu Thr Asp Lys Leu Leu Ile Arg
245 250 255
Val Ile Ile Gln Asn Asp Gly Thr Gly Lys Asp Lys Tyr Ile Glu Val
260 265 270
Ile Phe Gln Ala Leu Phe Leu Gly Leu Val Asp Glu Leu Ile Pro Leu
275 280 285
Leu Lys Glu Lys Phe Pro Glu Phe Asp Leu Glu Lys Lys Asp Cys Phe
290 295 300
Gln Glu Pro Val Val Asp Cys Ser Asn Arg Pro Cys Ile Lys Lys Glu
305 310 315 320
Cys Arg Glu Thr Thr Trp Ile Gly Ser Val Leu Phe Phe Tyr Gly Arg
325 330 335
Arg Thr Asn Glu Ser Leu Glu Val Leu Leu Asp Lys Ser Ile Pro Thr
340 345 350
Gln Lys Asn Tyr Asn Lys Gly Thr Ser Asp Phe Val Lys Thr Pro Ile
355 360 365
Pro Glu Ser Gly Trp Lys Met Val Gln Arg Leu Phe Leu Glu Glu Glu
370 375 380
Arg Pro Gln Met Ile Leu Glu Pro Leu Gly Gly Lys Leu Asp Glu Ile
385 390 395 400
Ser Lys Ser Glu Ile Ser Phe Pro His Arg Lys Gly Asn Leu Tyr Asn
405 410 415
Ile Gln Tyr Leu Val Asn Trp Gly Asp Asn Ser Glu Asn Val Ser Ser
420 425 430
Gln Lys Leu Ala Trp Met Arg Lys Leu Tyr Lys Asp Met Glu Pro Tyr
435 440 445
Val Ala Asn Ser Pro Arg Thr Ala Tyr Leu Asn Tyr Arg Asp Leu Asp
450 455 460
Phe Gly Ser Asn Asp Glu Gly Tyr Ser Tyr Ser Lys Ala Lys Ile Trp
465 470 475 480
Gly Glu Lys Tyr Phe Asn Gly Asn Phe Lys Arg Leu Ala Lys Val Lys
485 490 495
Ser Lys Val Asp Pro Asn Asn Phe Phe Arg Asn Glu Gln Ser Ile Pro
500 505 510
Pro Tyr Gln Ser His
515
<210> 60
<211> 1575
<212> DNA
<213> 人工序列
<220>
<223> CBS86-A0A1U8G0D2-无信号肽-strep标签
<220>
<221> CDS
<222> (1)..(1575)
<400> 60
atg tct caa gag gat ttc ttg gag tgt ttg tcc aag tac tct aag tcc 48
Met Ser Gln Glu Asp Phe Leu Glu Cys Leu Ser Lys Tyr Ser Lys Ser
1 5 10 15
aac gtt gaa caa aac gtt tac act cca act tct cca acc tac tcc tct 96
Asn Val Glu Gln Asn Val Tyr Thr Pro Thr Ser Pro Thr Tyr Ser Ser
20 25 30
att ttg gaa tac gct caa aag aac cca aga tgg gtt aat tct tct cac 144
Ile Leu Glu Tyr Ala Gln Lys Asn Pro Arg Trp Val Asn Ser Ser His
35 40 45
cca aac ttt att gcc tcg cca aga aaa gaa tct gac atc aag cca att 192
Pro Asn Phe Ile Ala Ser Pro Arg Lys Glu Ser Asp Ile Lys Pro Ile
50 55 60
atc ttg tgc gct aag aaa ttg ggc ttg cag att aag att aag tcc ggt 240
Ile Leu Cys Ala Lys Lys Leu Gly Leu Gln Ile Lys Ile Lys Ser Gly
65 70 75 80
ggt cat gat tac gag ggt att tct tac aga tct aag tcc cca ttc gtt 288
Gly His Asp Tyr Glu Gly Ile Ser Tyr Arg Ser Lys Ser Pro Phe Val
85 90 95
atg ttg gac ttg tct aac ttg aac aag atc gag atc aac tgg aag aaa 336
Met Leu Asp Leu Ser Asn Leu Asn Lys Ile Glu Ile Asn Trp Lys Lys
100 105 110
gaa acc gtt tgg gtt caa act ggt gct act ttg ggt caa ctg tat tat 384
Glu Thr Val Trp Val Gln Thr Gly Ala Thr Leu Gly Gln Leu Tyr Tyr
115 120 125
gct att gcc aac aag tct aac gtt cat ggt ttt cca ggt ggt gtt tgt 432
Ala Ile Ala Asn Lys Ser Asn Val His Gly Phe Pro Gly Gly Val Cys
130 135 140
ttc tct att ggt act ggt ggt att att tct ggt ggt ggt ata ggt act 480
Phe Ser Ile Gly Thr Gly Gly Ile Ile Ser Gly Gly Gly Ile Gly Thr
145 150 155 160
atg atg aga aaa ttt ggt gtt gcc gct gat aac gtt gtt gat gct aga 528
Met Met Arg Lys Phe Gly Val Ala Ala Asp Asn Val Val Asp Ala Arg
165 170 175
gtt atg gat gtc aac ggt aag atc ttg gac aag aaa aag atg aag gaa 576
Val Met Asp Val Asn Gly Lys Ile Leu Asp Lys Lys Lys Met Lys Glu
180 185 190
gat ttg ttc tgg gcc att aga ggt ggt ggt ggc gct tct ttt ggt gtt 624
Asp Leu Phe Trp Ala Ile Arg Gly Gly Gly Gly Ala Ser Phe Gly Val
195 200 205
att ttg gct tgg aaa ttg aag ttg gtt agg gtt cca gaa aag gtc act 672
Ile Leu Ala Trp Lys Leu Lys Leu Val Arg Val Pro Glu Lys Val Thr
210 215 220
gtt ttt aac gtc tac aaa aag ttg gaa ggc aac cag aac ttg ttg caa 720
Val Phe Asn Val Tyr Lys Lys Leu Glu Gly Asn Gln Asn Leu Leu Gln
225 230 235 240
aag tgg gaa aac att gct cat caa ttg acc gac aag ttg ttg atc aga 768
Lys Trp Glu Asn Ile Ala His Gln Leu Thr Asp Lys Leu Leu Ile Arg
245 250 255
gtc atc att caa aac gac ggt act ggt aag gat aag tac atc gaa gtt 816
Val Ile Ile Gln Asn Asp Gly Thr Gly Lys Asp Lys Tyr Ile Glu Val
260 265 270
atc ttc caa gcc ttg ttc ttg ggt tta gtt gat gaa ttg atc ccc ttg 864
Ile Phe Gln Ala Leu Phe Leu Gly Leu Val Asp Glu Leu Ile Pro Leu
275 280 285
ctg aaa gaa aag ttc cca gaa ttc gat ttg gaa aag aag gac tgt ttc 912
Leu Lys Glu Lys Phe Pro Glu Phe Asp Leu Glu Lys Lys Asp Cys Phe
290 295 300
caa gaa cca gtt gtt gac tgt tct aac aga cca tgc att aag aaa gaa 960
Gln Glu Pro Val Val Asp Cys Ser Asn Arg Pro Cys Ile Lys Lys Glu
305 310 315 320
tgc aga gaa act acc tgg atc ggt tct gtt ttg ttt ttc tac ggt aga 1008
Cys Arg Glu Thr Thr Trp Ile Gly Ser Val Leu Phe Phe Tyr Gly Arg
325 330 335
agg acc aac gaa tcc ttg gaa gtt ttg ttg gat aag tcc att cca acg 1056
Arg Thr Asn Glu Ser Leu Glu Val Leu Leu Asp Lys Ser Ile Pro Thr
340 345 350
cag aag aac tac aac aaa ggt act tct gat ttc gtc aag acc cca att 1104
Gln Lys Asn Tyr Asn Lys Gly Thr Ser Asp Phe Val Lys Thr Pro Ile
355 360 365
cca gaa tct ggt tgg aaa atg gtt caa agg ttg ttc tta gaa gag gaa 1152
Pro Glu Ser Gly Trp Lys Met Val Gln Arg Leu Phe Leu Glu Glu Glu
370 375 380
cgt cca caa atg atc ttg gaa cca tta ggt ggt aag ttg gac gaa atc 1200
Arg Pro Gln Met Ile Leu Glu Pro Leu Gly Gly Lys Leu Asp Glu Ile
385 390 395 400
tcc aaa tcc gaa att tca ttc cca cac aga aag ggc aac ttg tac aac 1248
Ser Lys Ser Glu Ile Ser Phe Pro His Arg Lys Gly Asn Leu Tyr Asn
405 410 415
att caa tac ttg gtt aac tgg ggt gac aac tcc gaa aat gtt tcc tct 1296
Ile Gln Tyr Leu Val Asn Trp Gly Asp Asn Ser Glu Asn Val Ser Ser
420 425 430
caa aaa ttg gcc tgg atg cgt aag ttg tac aaa gat atg gaa cca tac 1344
Gln Lys Leu Ala Trp Met Arg Lys Leu Tyr Lys Asp Met Glu Pro Tyr
435 440 445
gtt gcc aac tct cca aga act gct tat ttg aac tac aga gat ttg gac 1392
Val Ala Asn Ser Pro Arg Thr Ala Tyr Leu Asn Tyr Arg Asp Leu Asp
450 455 460
ttc ggt tcc aat gat gaa ggt tac tct tac tcc aaa gct aag att tgg 1440
Phe Gly Ser Asn Asp Glu Gly Tyr Ser Tyr Ser Lys Ala Lys Ile Trp
465 470 475 480
ggt gaa aag tac ttc aac ggc aac ttt aag aga ttg gcc aag gtt aag 1488
Gly Glu Lys Tyr Phe Asn Gly Asn Phe Lys Arg Leu Ala Lys Val Lys
485 490 495
tct aag gtc gat cca aac aat ttc ttc aga aac gaa caa tcg atc cca 1536
Ser Lys Val Asp Pro Asn Asn Phe Phe Arg Asn Glu Gln Ser Ile Pro
500 505 510
cca tat caa tcc cat tgg tca cat cca caa ttt gaa aag 1575
Pro Tyr Gln Ser His Trp Ser His Pro Gln Phe Glu Lys
515 520 525
<210> 61
<211> 525
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 61
Met Ser Gln Glu Asp Phe Leu Glu Cys Leu Ser Lys Tyr Ser Lys Ser
1 5 10 15
Asn Val Glu Gln Asn Val Tyr Thr Pro Thr Ser Pro Thr Tyr Ser Ser
20 25 30
Ile Leu Glu Tyr Ala Gln Lys Asn Pro Arg Trp Val Asn Ser Ser His
35 40 45
Pro Asn Phe Ile Ala Ser Pro Arg Lys Glu Ser Asp Ile Lys Pro Ile
50 55 60
Ile Leu Cys Ala Lys Lys Leu Gly Leu Gln Ile Lys Ile Lys Ser Gly
65 70 75 80
Gly His Asp Tyr Glu Gly Ile Ser Tyr Arg Ser Lys Ser Pro Phe Val
85 90 95
Met Leu Asp Leu Ser Asn Leu Asn Lys Ile Glu Ile Asn Trp Lys Lys
100 105 110
Glu Thr Val Trp Val Gln Thr Gly Ala Thr Leu Gly Gln Leu Tyr Tyr
115 120 125
Ala Ile Ala Asn Lys Ser Asn Val His Gly Phe Pro Gly Gly Val Cys
130 135 140
Phe Ser Ile Gly Thr Gly Gly Ile Ile Ser Gly Gly Gly Ile Gly Thr
145 150 155 160
Met Met Arg Lys Phe Gly Val Ala Ala Asp Asn Val Val Asp Ala Arg
165 170 175
Val Met Asp Val Asn Gly Lys Ile Leu Asp Lys Lys Lys Met Lys Glu
180 185 190
Asp Leu Phe Trp Ala Ile Arg Gly Gly Gly Gly Ala Ser Phe Gly Val
195 200 205
Ile Leu Ala Trp Lys Leu Lys Leu Val Arg Val Pro Glu Lys Val Thr
210 215 220
Val Phe Asn Val Tyr Lys Lys Leu Glu Gly Asn Gln Asn Leu Leu Gln
225 230 235 240
Lys Trp Glu Asn Ile Ala His Gln Leu Thr Asp Lys Leu Leu Ile Arg
245 250 255
Val Ile Ile Gln Asn Asp Gly Thr Gly Lys Asp Lys Tyr Ile Glu Val
260 265 270
Ile Phe Gln Ala Leu Phe Leu Gly Leu Val Asp Glu Leu Ile Pro Leu
275 280 285
Leu Lys Glu Lys Phe Pro Glu Phe Asp Leu Glu Lys Lys Asp Cys Phe
290 295 300
Gln Glu Pro Val Val Asp Cys Ser Asn Arg Pro Cys Ile Lys Lys Glu
305 310 315 320
Cys Arg Glu Thr Thr Trp Ile Gly Ser Val Leu Phe Phe Tyr Gly Arg
325 330 335
Arg Thr Asn Glu Ser Leu Glu Val Leu Leu Asp Lys Ser Ile Pro Thr
340 345 350
Gln Lys Asn Tyr Asn Lys Gly Thr Ser Asp Phe Val Lys Thr Pro Ile
355 360 365
Pro Glu Ser Gly Trp Lys Met Val Gln Arg Leu Phe Leu Glu Glu Glu
370 375 380
Arg Pro Gln Met Ile Leu Glu Pro Leu Gly Gly Lys Leu Asp Glu Ile
385 390 395 400
Ser Lys Ser Glu Ile Ser Phe Pro His Arg Lys Gly Asn Leu Tyr Asn
405 410 415
Ile Gln Tyr Leu Val Asn Trp Gly Asp Asn Ser Glu Asn Val Ser Ser
420 425 430
Gln Lys Leu Ala Trp Met Arg Lys Leu Tyr Lys Asp Met Glu Pro Tyr
435 440 445
Val Ala Asn Ser Pro Arg Thr Ala Tyr Leu Asn Tyr Arg Asp Leu Asp
450 455 460
Phe Gly Ser Asn Asp Glu Gly Tyr Ser Tyr Ser Lys Ala Lys Ile Trp
465 470 475 480
Gly Glu Lys Tyr Phe Asn Gly Asn Phe Lys Arg Leu Ala Lys Val Lys
485 490 495
Ser Lys Val Asp Pro Asn Asn Phe Phe Arg Asn Glu Gln Ser Ile Pro
500 505 510
Pro Tyr Gln Ser His Trp Ser His Pro Gln Phe Glu Lys
515 520 525
<210> 62
<211> 1632
<212> DNA
<213> 人工序列
<220>
<223> CBS86-A0A1U8G0D2-截断alpha因子-无信号肽-strep标签
<220>
<221> CDS
<222> (1)..(1632)
<400> 62
atg aga ttt cct tcc atc ttc acg gct gtg cta ttt gca gca tcc tcc 48
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser
1 5 10 15
gca ctt gca atg tct caa gag gat ttc ttg gag tgt ttg tcc aag tac 96
Ala Leu Ala Met Ser Gln Glu Asp Phe Leu Glu Cys Leu Ser Lys Tyr
20 25 30
tct aag tcc aac gtt gaa caa aac gtt tac act cca act tct cca acc 144
Ser Lys Ser Asn Val Glu Gln Asn Val Tyr Thr Pro Thr Ser Pro Thr
35 40 45
tac tcc tct att ttg gaa tac gct caa aag aac cca aga tgg gtt aat 192
Tyr Ser Ser Ile Leu Glu Tyr Ala Gln Lys Asn Pro Arg Trp Val Asn
50 55 60
tct tct cac cca aac ttt att gcc tcg cca aga aaa gaa tct gac atc 240
Ser Ser His Pro Asn Phe Ile Ala Ser Pro Arg Lys Glu Ser Asp Ile
65 70 75 80
aag cca att atc ttg tgc gct aag aaa ttg ggc ttg cag att aag att 288
Lys Pro Ile Ile Leu Cys Ala Lys Lys Leu Gly Leu Gln Ile Lys Ile
85 90 95
aag tcc ggt ggt cat gat tac gag ggt att tct tac aga tct aag tcc 336
Lys Ser Gly Gly His Asp Tyr Glu Gly Ile Ser Tyr Arg Ser Lys Ser
100 105 110
cca ttc gtt atg ttg gac ttg tct aac ttg aac aag atc gag atc aac 384
Pro Phe Val Met Leu Asp Leu Ser Asn Leu Asn Lys Ile Glu Ile Asn
115 120 125
tgg aag aaa gaa acc gtt tgg gtt caa act ggt gct act ttg ggt caa 432
Trp Lys Lys Glu Thr Val Trp Val Gln Thr Gly Ala Thr Leu Gly Gln
130 135 140
ctg tat tat gct att gcc aac aag tct aac gtt cat ggt ttt cca ggt 480
Leu Tyr Tyr Ala Ile Ala Asn Lys Ser Asn Val His Gly Phe Pro Gly
145 150 155 160
ggt gtt tgt ttc tct att ggt act ggt ggt att att tct ggt ggt ggt 528
Gly Val Cys Phe Ser Ile Gly Thr Gly Gly Ile Ile Ser Gly Gly Gly
165 170 175
ata ggt act atg atg aga aaa ttt ggt gtt gcc gct gat aac gtt gtt 576
Ile Gly Thr Met Met Arg Lys Phe Gly Val Ala Ala Asp Asn Val Val
180 185 190
gat gct aga gtt atg gat gtc aac ggt aag atc ttg gac aag aaa aag 624
Asp Ala Arg Val Met Asp Val Asn Gly Lys Ile Leu Asp Lys Lys Lys
195 200 205
atg aag gaa gat ttg ttc tgg gcc att aga ggt ggt ggt ggc gct tct 672
Met Lys Glu Asp Leu Phe Trp Ala Ile Arg Gly Gly Gly Gly Ala Ser
210 215 220
ttt ggt gtt att ttg gct tgg aaa ttg aag ttg gtt agg gtt cca gaa 720
Phe Gly Val Ile Leu Ala Trp Lys Leu Lys Leu Val Arg Val Pro Glu
225 230 235 240
aag gtc act gtt ttt aac gtc tac aaa aag ttg gaa ggc aac cag aac 768
Lys Val Thr Val Phe Asn Val Tyr Lys Lys Leu Glu Gly Asn Gln Asn
245 250 255
ttg ttg caa aag tgg gaa aac att gct cat caa ttg acc gac aag ttg 816
Leu Leu Gln Lys Trp Glu Asn Ile Ala His Gln Leu Thr Asp Lys Leu
260 265 270
ttg atc aga gtc atc att caa aac gac ggt act ggt aag gat aag tac 864
Leu Ile Arg Val Ile Ile Gln Asn Asp Gly Thr Gly Lys Asp Lys Tyr
275 280 285
atc gaa gtt atc ttc caa gcc ttg ttc ttg ggt tta gtt gat gaa ttg 912
Ile Glu Val Ile Phe Gln Ala Leu Phe Leu Gly Leu Val Asp Glu Leu
290 295 300
atc ccc ttg ctg aaa gaa aag ttc cca gaa ttc gat ttg gaa aag aag 960
Ile Pro Leu Leu Lys Glu Lys Phe Pro Glu Phe Asp Leu Glu Lys Lys
305 310 315 320
gac tgt ttc caa gaa cca gtt gtt gac tgt tct aac aga cca tgc att 1008
Asp Cys Phe Gln Glu Pro Val Val Asp Cys Ser Asn Arg Pro Cys Ile
325 330 335
aag aaa gaa tgc aga gaa act acc tgg atc ggt tct gtt ttg ttt ttc 1056
Lys Lys Glu Cys Arg Glu Thr Thr Trp Ile Gly Ser Val Leu Phe Phe
340 345 350
tac ggt aga agg acc aac gaa tcc ttg gaa gtt ttg ttg gat aag tcc 1104
Tyr Gly Arg Arg Thr Asn Glu Ser Leu Glu Val Leu Leu Asp Lys Ser
355 360 365
att cca acg cag aag aac tac aac aaa ggt act tct gat ttc gtc aag 1152
Ile Pro Thr Gln Lys Asn Tyr Asn Lys Gly Thr Ser Asp Phe Val Lys
370 375 380
acc cca att cca gaa tct ggt tgg aaa atg gtt caa agg ttg ttc tta 1200
Thr Pro Ile Pro Glu Ser Gly Trp Lys Met Val Gln Arg Leu Phe Leu
385 390 395 400
gaa gag gaa cgt cca caa atg atc ttg gaa cca tta ggt ggt aag ttg 1248
Glu Glu Glu Arg Pro Gln Met Ile Leu Glu Pro Leu Gly Gly Lys Leu
405 410 415
gac gaa atc tcc aaa tcc gaa att tca ttc cca cac aga aag ggc aac 1296
Asp Glu Ile Ser Lys Ser Glu Ile Ser Phe Pro His Arg Lys Gly Asn
420 425 430
ttg tac aac att caa tac ttg gtt aac tgg ggt gac aac tcc gaa aat 1344
Leu Tyr Asn Ile Gln Tyr Leu Val Asn Trp Gly Asp Asn Ser Glu Asn
435 440 445
gtt tcc tct caa aaa ttg gcc tgg atg cgt aag ttg tac aaa gat atg 1392
Val Ser Ser Gln Lys Leu Ala Trp Met Arg Lys Leu Tyr Lys Asp Met
450 455 460
gaa cca tac gtt gcc aac tct cca aga act gct tat ttg aac tac aga 1440
Glu Pro Tyr Val Ala Asn Ser Pro Arg Thr Ala Tyr Leu Asn Tyr Arg
465 470 475 480
gat ttg gac ttc ggt tcc aat gat gaa ggt tac tct tac tcc aaa gct 1488
Asp Leu Asp Phe Gly Ser Asn Asp Glu Gly Tyr Ser Tyr Ser Lys Ala
485 490 495
aag att tgg ggt gaa aag tac ttc aac ggc aac ttt aag aga ttg gcc 1536
Lys Ile Trp Gly Glu Lys Tyr Phe Asn Gly Asn Phe Lys Arg Leu Ala
500 505 510
aag gtt aag tct aag gtc gat cca aac aat ttc ttc aga aac gaa caa 1584
Lys Val Lys Ser Lys Val Asp Pro Asn Asn Phe Phe Arg Asn Glu Gln
515 520 525
tcg atc cca cca tat caa tcc cat tgg tca cat cca caa ttt gaa aag 1632
Ser Ile Pro Pro Tyr Gln Ser His Trp Ser His Pro Gln Phe Glu Lys
530 535 540
<210> 63
<211> 544
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 63
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser
1 5 10 15
Ala Leu Ala Met Ser Gln Glu Asp Phe Leu Glu Cys Leu Ser Lys Tyr
20 25 30
Ser Lys Ser Asn Val Glu Gln Asn Val Tyr Thr Pro Thr Ser Pro Thr
35 40 45
Tyr Ser Ser Ile Leu Glu Tyr Ala Gln Lys Asn Pro Arg Trp Val Asn
50 55 60
Ser Ser His Pro Asn Phe Ile Ala Ser Pro Arg Lys Glu Ser Asp Ile
65 70 75 80
Lys Pro Ile Ile Leu Cys Ala Lys Lys Leu Gly Leu Gln Ile Lys Ile
85 90 95
Lys Ser Gly Gly His Asp Tyr Glu Gly Ile Ser Tyr Arg Ser Lys Ser
100 105 110
Pro Phe Val Met Leu Asp Leu Ser Asn Leu Asn Lys Ile Glu Ile Asn
115 120 125
Trp Lys Lys Glu Thr Val Trp Val Gln Thr Gly Ala Thr Leu Gly Gln
130 135 140
Leu Tyr Tyr Ala Ile Ala Asn Lys Ser Asn Val His Gly Phe Pro Gly
145 150 155 160
Gly Val Cys Phe Ser Ile Gly Thr Gly Gly Ile Ile Ser Gly Gly Gly
165 170 175
Ile Gly Thr Met Met Arg Lys Phe Gly Val Ala Ala Asp Asn Val Val
180 185 190
Asp Ala Arg Val Met Asp Val Asn Gly Lys Ile Leu Asp Lys Lys Lys
195 200 205
Met Lys Glu Asp Leu Phe Trp Ala Ile Arg Gly Gly Gly Gly Ala Ser
210 215 220
Phe Gly Val Ile Leu Ala Trp Lys Leu Lys Leu Val Arg Val Pro Glu
225 230 235 240
Lys Val Thr Val Phe Asn Val Tyr Lys Lys Leu Glu Gly Asn Gln Asn
245 250 255
Leu Leu Gln Lys Trp Glu Asn Ile Ala His Gln Leu Thr Asp Lys Leu
260 265 270
Leu Ile Arg Val Ile Ile Gln Asn Asp Gly Thr Gly Lys Asp Lys Tyr
275 280 285
Ile Glu Val Ile Phe Gln Ala Leu Phe Leu Gly Leu Val Asp Glu Leu
290 295 300
Ile Pro Leu Leu Lys Glu Lys Phe Pro Glu Phe Asp Leu Glu Lys Lys
305 310 315 320
Asp Cys Phe Gln Glu Pro Val Val Asp Cys Ser Asn Arg Pro Cys Ile
325 330 335
Lys Lys Glu Cys Arg Glu Thr Thr Trp Ile Gly Ser Val Leu Phe Phe
340 345 350
Tyr Gly Arg Arg Thr Asn Glu Ser Leu Glu Val Leu Leu Asp Lys Ser
355 360 365
Ile Pro Thr Gln Lys Asn Tyr Asn Lys Gly Thr Ser Asp Phe Val Lys
370 375 380
Thr Pro Ile Pro Glu Ser Gly Trp Lys Met Val Gln Arg Leu Phe Leu
385 390 395 400
Glu Glu Glu Arg Pro Gln Met Ile Leu Glu Pro Leu Gly Gly Lys Leu
405 410 415
Asp Glu Ile Ser Lys Ser Glu Ile Ser Phe Pro His Arg Lys Gly Asn
420 425 430
Leu Tyr Asn Ile Gln Tyr Leu Val Asn Trp Gly Asp Asn Ser Glu Asn
435 440 445
Val Ser Ser Gln Lys Leu Ala Trp Met Arg Lys Leu Tyr Lys Asp Met
450 455 460
Glu Pro Tyr Val Ala Asn Ser Pro Arg Thr Ala Tyr Leu Asn Tyr Arg
465 470 475 480
Asp Leu Asp Phe Gly Ser Asn Asp Glu Gly Tyr Ser Tyr Ser Lys Ala
485 490 495
Lys Ile Trp Gly Glu Lys Tyr Phe Asn Gly Asn Phe Lys Arg Leu Ala
500 505 510
Lys Val Lys Ser Lys Val Asp Pro Asn Asn Phe Phe Arg Asn Glu Gln
515 520 525
Ser Ile Pro Pro Tyr Gln Ser His Trp Ser His Pro Gln Phe Glu Lys
530 535 540
<210> 64
<211> 1842
<212> DNA
<213> 人工序列
<220>
<223> CBS86-A0A1U8G0D2-全长alpha因子-无信号肽-strep标签
<220>
<221> CDS
<222> (1)..(1842)
<400> 64
atg aga ttt cct tca att ttt act gct gtt tta ttc gca gca tcc tcc 48
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser
1 5 10 15
gca tta gct gct cca gtc aac act aca aca gaa gat gaa acg gca caa 96
Ala Leu Ala Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln
20 25 30
att ccg gct gaa gct gtc atc ggt tac tca gat tta gaa ggg gat ttc 144
Ile Pro Ala Glu Ala Val Ile Gly Tyr Ser Asp Leu Glu Gly Asp Phe
35 40 45
gat gtt gct gtt ttg cca ttt tcc aac agc aca aat aac ggg tta ttg 192
Asp Val Ala Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu
50 55 60
ttt ata aat act act att gcc agc att gct gct aaa gaa gaa ggg gta 240
Phe Ile Asn Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val
65 70 75 80
tct ctc gag aaa aga gag gct gaa gct atg tct caa gag gat ttc ttg 288
Ser Leu Glu Lys Arg Glu Ala Glu Ala Met Ser Gln Glu Asp Phe Leu
85 90 95
gag tgt ttg tcc aag tac tct aag tcc aac gtt gaa caa aac gtt tac 336
Glu Cys Leu Ser Lys Tyr Ser Lys Ser Asn Val Glu Gln Asn Val Tyr
100 105 110
act cca act tct cca acc tac tcc tct att ttg gaa tac gct caa aag 384
Thr Pro Thr Ser Pro Thr Tyr Ser Ser Ile Leu Glu Tyr Ala Gln Lys
115 120 125
aac cca aga tgg gtt aat tct tct cac cca aac ttt att gcc tcg cca 432
Asn Pro Arg Trp Val Asn Ser Ser His Pro Asn Phe Ile Ala Ser Pro
130 135 140
aga aaa gaa tct gac atc aag cca att atc ttg tgc gct aag aaa ttg 480
Arg Lys Glu Ser Asp Ile Lys Pro Ile Ile Leu Cys Ala Lys Lys Leu
145 150 155 160
ggc ttg cag att aag att aag tcc ggt ggt cat gat tac gag ggt att 528
Gly Leu Gln Ile Lys Ile Lys Ser Gly Gly His Asp Tyr Glu Gly Ile
165 170 175
tct tac aga tct aag tcc cca ttc gtt atg ttg gac ttg tct aac ttg 576
Ser Tyr Arg Ser Lys Ser Pro Phe Val Met Leu Asp Leu Ser Asn Leu
180 185 190
aac aag atc gag atc aac tgg aag aaa gaa acc gtt tgg gtt caa act 624
Asn Lys Ile Glu Ile Asn Trp Lys Lys Glu Thr Val Trp Val Gln Thr
195 200 205
ggt gct act ttg ggt caa ctg tat tat gct att gcc aac aag tct aac 672
Gly Ala Thr Leu Gly Gln Leu Tyr Tyr Ala Ile Ala Asn Lys Ser Asn
210 215 220
gtt cat ggt ttt cca ggt ggt gtt tgt ttc tct att ggt act ggt ggt 720
Val His Gly Phe Pro Gly Gly Val Cys Phe Ser Ile Gly Thr Gly Gly
225 230 235 240
att att tct ggt ggt ggt ata ggt act atg atg aga aaa ttt ggt gtt 768
Ile Ile Ser Gly Gly Gly Ile Gly Thr Met Met Arg Lys Phe Gly Val
245 250 255
gcc gct gat aac gtt gtt gat gct aga gtt atg gat gtc aac ggt aag 816
Ala Ala Asp Asn Val Val Asp Ala Arg Val Met Asp Val Asn Gly Lys
260 265 270
atc ttg gac aag aaa aag atg aag gaa gat ttg ttc tgg gcc att aga 864
Ile Leu Asp Lys Lys Lys Met Lys Glu Asp Leu Phe Trp Ala Ile Arg
275 280 285
ggt ggt ggt ggc gct tct ttt ggt gtt att ttg gct tgg aaa ttg aag 912
Gly Gly Gly Gly Ala Ser Phe Gly Val Ile Leu Ala Trp Lys Leu Lys
290 295 300
ttg gtt agg gtt cca gaa aag gtc act gtt ttt aac gtc tac aaa aag 960
Leu Val Arg Val Pro Glu Lys Val Thr Val Phe Asn Val Tyr Lys Lys
305 310 315 320
ttg gaa ggc aac cag aac ttg ttg caa aag tgg gaa aac att gct cat 1008
Leu Glu Gly Asn Gln Asn Leu Leu Gln Lys Trp Glu Asn Ile Ala His
325 330 335
caa ttg acc gac aag ttg ttg atc aga gtc atc att caa aac gac ggt 1056
Gln Leu Thr Asp Lys Leu Leu Ile Arg Val Ile Ile Gln Asn Asp Gly
340 345 350
act ggt aag gat aag tac atc gaa gtt atc ttc caa gcc ttg ttc ttg 1104
Thr Gly Lys Asp Lys Tyr Ile Glu Val Ile Phe Gln Ala Leu Phe Leu
355 360 365
ggt tta gtt gat gaa ttg atc ccc ttg ctg aaa gaa aag ttc cca gaa 1152
Gly Leu Val Asp Glu Leu Ile Pro Leu Leu Lys Glu Lys Phe Pro Glu
370 375 380
ttc gat ttg gaa aag aag gac tgt ttc caa gaa cca gtt gtt gac tgt 1200
Phe Asp Leu Glu Lys Lys Asp Cys Phe Gln Glu Pro Val Val Asp Cys
385 390 395 400
tct aac aga cca tgc att aag aaa gaa tgc aga gaa act acc tgg atc 1248
Ser Asn Arg Pro Cys Ile Lys Lys Glu Cys Arg Glu Thr Thr Trp Ile
405 410 415
ggt tct gtt ttg ttt ttc tac ggt aga agg acc aac gaa tcc ttg gaa 1296
Gly Ser Val Leu Phe Phe Tyr Gly Arg Arg Thr Asn Glu Ser Leu Glu
420 425 430
gtt ttg ttg gat aag tcc att cca acg cag aag aac tac aac aaa ggt 1344
Val Leu Leu Asp Lys Ser Ile Pro Thr Gln Lys Asn Tyr Asn Lys Gly
435 440 445
act tct gat ttc gtc aag acc cca att cca gaa tct ggt tgg aaa atg 1392
Thr Ser Asp Phe Val Lys Thr Pro Ile Pro Glu Ser Gly Trp Lys Met
450 455 460
gtt caa agg ttg ttc tta gaa gag gaa cgt cca caa atg atc ttg gaa 1440
Val Gln Arg Leu Phe Leu Glu Glu Glu Arg Pro Gln Met Ile Leu Glu
465 470 475 480
cca tta ggt ggt aag ttg gac gaa atc tcc aaa tcc gaa att tca ttc 1488
Pro Leu Gly Gly Lys Leu Asp Glu Ile Ser Lys Ser Glu Ile Ser Phe
485 490 495
cca cac aga aag ggc aac ttg tac aac att caa tac ttg gtt aac tgg 1536
Pro His Arg Lys Gly Asn Leu Tyr Asn Ile Gln Tyr Leu Val Asn Trp
500 505 510
ggt gac aac tcc gaa aat gtt tcc tct caa aaa ttg gcc tgg atg cgt 1584
Gly Asp Asn Ser Glu Asn Val Ser Ser Gln Lys Leu Ala Trp Met Arg
515 520 525
aag ttg tac aaa gat atg gaa cca tac gtt gcc aac tct cca aga act 1632
Lys Leu Tyr Lys Asp Met Glu Pro Tyr Val Ala Asn Ser Pro Arg Thr
530 535 540
gct tat ttg aac tac aga gat ttg gac ttc ggt tcc aat gat gaa ggt 1680
Ala Tyr Leu Asn Tyr Arg Asp Leu Asp Phe Gly Ser Asn Asp Glu Gly
545 550 555 560
tac tct tac tcc aaa gct aag att tgg ggt gaa aag tac ttc aac ggc 1728
Tyr Ser Tyr Ser Lys Ala Lys Ile Trp Gly Glu Lys Tyr Phe Asn Gly
565 570 575
aac ttt aag aga ttg gcc aag gtt aag tct aag gtc gat cca aac aat 1776
Asn Phe Lys Arg Leu Ala Lys Val Lys Ser Lys Val Asp Pro Asn Asn
580 585 590
ttc ttc aga aac gaa caa tcg atc cca cca tat caa tcc cat tgg tca 1824
Phe Phe Arg Asn Glu Gln Ser Ile Pro Pro Tyr Gln Ser His Trp Ser
595 600 605
cat cca caa ttt gaa aag 1842
His Pro Gln Phe Glu Lys
610
<210> 65
<211> 614
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 65
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser
1 5 10 15
Ala Leu Ala Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln
20 25 30
Ile Pro Ala Glu Ala Val Ile Gly Tyr Ser Asp Leu Glu Gly Asp Phe
35 40 45
Asp Val Ala Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu
50 55 60
Phe Ile Asn Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val
65 70 75 80
Ser Leu Glu Lys Arg Glu Ala Glu Ala Met Ser Gln Glu Asp Phe Leu
85 90 95
Glu Cys Leu Ser Lys Tyr Ser Lys Ser Asn Val Glu Gln Asn Val Tyr
100 105 110
Thr Pro Thr Ser Pro Thr Tyr Ser Ser Ile Leu Glu Tyr Ala Gln Lys
115 120 125
Asn Pro Arg Trp Val Asn Ser Ser His Pro Asn Phe Ile Ala Ser Pro
130 135 140
Arg Lys Glu Ser Asp Ile Lys Pro Ile Ile Leu Cys Ala Lys Lys Leu
145 150 155 160
Gly Leu Gln Ile Lys Ile Lys Ser Gly Gly His Asp Tyr Glu Gly Ile
165 170 175
Ser Tyr Arg Ser Lys Ser Pro Phe Val Met Leu Asp Leu Ser Asn Leu
180 185 190
Asn Lys Ile Glu Ile Asn Trp Lys Lys Glu Thr Val Trp Val Gln Thr
195 200 205
Gly Ala Thr Leu Gly Gln Leu Tyr Tyr Ala Ile Ala Asn Lys Ser Asn
210 215 220
Val His Gly Phe Pro Gly Gly Val Cys Phe Ser Ile Gly Thr Gly Gly
225 230 235 240
Ile Ile Ser Gly Gly Gly Ile Gly Thr Met Met Arg Lys Phe Gly Val
245 250 255
Ala Ala Asp Asn Val Val Asp Ala Arg Val Met Asp Val Asn Gly Lys
260 265 270
Ile Leu Asp Lys Lys Lys Met Lys Glu Asp Leu Phe Trp Ala Ile Arg
275 280 285
Gly Gly Gly Gly Ala Ser Phe Gly Val Ile Leu Ala Trp Lys Leu Lys
290 295 300
Leu Val Arg Val Pro Glu Lys Val Thr Val Phe Asn Val Tyr Lys Lys
305 310 315 320
Leu Glu Gly Asn Gln Asn Leu Leu Gln Lys Trp Glu Asn Ile Ala His
325 330 335
Gln Leu Thr Asp Lys Leu Leu Ile Arg Val Ile Ile Gln Asn Asp Gly
340 345 350
Thr Gly Lys Asp Lys Tyr Ile Glu Val Ile Phe Gln Ala Leu Phe Leu
355 360 365
Gly Leu Val Asp Glu Leu Ile Pro Leu Leu Lys Glu Lys Phe Pro Glu
370 375 380
Phe Asp Leu Glu Lys Lys Asp Cys Phe Gln Glu Pro Val Val Asp Cys
385 390 395 400
Ser Asn Arg Pro Cys Ile Lys Lys Glu Cys Arg Glu Thr Thr Trp Ile
405 410 415
Gly Ser Val Leu Phe Phe Tyr Gly Arg Arg Thr Asn Glu Ser Leu Glu
420 425 430
Val Leu Leu Asp Lys Ser Ile Pro Thr Gln Lys Asn Tyr Asn Lys Gly
435 440 445
Thr Ser Asp Phe Val Lys Thr Pro Ile Pro Glu Ser Gly Trp Lys Met
450 455 460
Val Gln Arg Leu Phe Leu Glu Glu Glu Arg Pro Gln Met Ile Leu Glu
465 470 475 480
Pro Leu Gly Gly Lys Leu Asp Glu Ile Ser Lys Ser Glu Ile Ser Phe
485 490 495
Pro His Arg Lys Gly Asn Leu Tyr Asn Ile Gln Tyr Leu Val Asn Trp
500 505 510
Gly Asp Asn Ser Glu Asn Val Ser Ser Gln Lys Leu Ala Trp Met Arg
515 520 525
Lys Leu Tyr Lys Asp Met Glu Pro Tyr Val Ala Asn Ser Pro Arg Thr
530 535 540
Ala Tyr Leu Asn Tyr Arg Asp Leu Asp Phe Gly Ser Asn Asp Glu Gly
545 550 555 560
Tyr Ser Tyr Ser Lys Ala Lys Ile Trp Gly Glu Lys Tyr Phe Asn Gly
565 570 575
Asn Phe Lys Arg Leu Ala Lys Val Lys Ser Lys Val Asp Pro Asn Asn
580 585 590
Phe Phe Arg Asn Glu Gln Ser Ile Pro Pro Tyr Gln Ser His Trp Ser
595 600 605
His Pro Gln Phe Glu Lys
610
<210> 66
<211> 1629
<212> DNA
<213> 人工序列
<220>
<223> CBS94-A0A1S3XA06
<220>
<221> CDS
<222> (1)..(1629)
<400> 66
atg ggt aga ttg cag ttg ttc tct tgc ttg ttg gtt tcc ttc ttg ttg 48
Met Gly Arg Leu Gln Leu Phe Ser Cys Leu Leu Val Ser Phe Leu Leu
1 5 10 15
ttg att gaa gct aac gcc caa caa gag ttc ttg caa tgt atc tac tct 96
Leu Ile Glu Ala Asn Ala Gln Gln Glu Phe Leu Gln Cys Ile Tyr Ser
20 25 30
cac tcc tcc gag aat att aag aag cac att cat ttc cca aac agc cca 144
His Ser Ser Glu Asn Ile Lys Lys His Ile His Phe Pro Asn Ser Pro
35 40 45
act tac tct tcg ttg ttg gaa tac gct caa aag aat cca aga tgg ctg 192
Thr Tyr Ser Ser Leu Leu Glu Tyr Ala Gln Lys Asn Pro Arg Trp Leu
50 55 60
aat tct tct ttc gcc cat cca att ttc att atc gct cca aag aac gaa 240
Asn Ser Ser Phe Ala His Pro Ile Phe Ile Ile Ala Pro Lys Asn Glu
65 70 75 80
tcc gaa atc cag cca att atc ttg tgc tct caa aag ttc ggc ttg caa 288
Ser Glu Ile Gln Pro Ile Ile Leu Cys Ser Gln Lys Phe Gly Leu Gln
85 90 95
atc aga gtt aag tct ggt ggt cat gat tac gaa ggt ttg tct ttc aga 336
Ile Arg Val Lys Ser Gly Gly His Asp Tyr Glu Gly Leu Ser Phe Arg
100 105 110
tct gtc act aac acc caa ttc gtt atc ttg gac ttg atc aac ttg gac 384
Ser Val Thr Asn Thr Gln Phe Val Ile Leu Asp Leu Ile Asn Leu Asp
115 120 125
gat atc aga atc gac aag aac gaa gaa act gct tgg att caa act ggt 432
Asp Ile Arg Ile Asp Lys Asn Glu Glu Thr Ala Trp Ile Gln Thr Gly
130 135 140
gtt act atg ggt cag ttg tac tac gaa att gcc aag aag tct aag atc 480
Val Thr Met Gly Gln Leu Tyr Tyr Glu Ile Ala Lys Lys Ser Lys Ile
145 150 155 160
ttg gct ttt cca ggt ggt cta tat cca tct gtt ggt tct ggt ggt ttg 528
Leu Ala Phe Pro Gly Gly Leu Tyr Pro Ser Val Gly Ser Gly Gly Leu
165 170 175
att tca ggt ggt ggt att ggt act ttg atg aga aag ttt ggt ttg tcc 576
Ile Ser Gly Gly Gly Ile Gly Thr Leu Met Arg Lys Phe Gly Leu Ser
180 185 190
gct gat aac gtc att gat gct aga gtt atg gat gtc aac ggt aga atc 624
Ala Asp Asn Val Ile Asp Ala Arg Val Met Asp Val Asn Gly Arg Ile
195 200 205
ttg gac aaa aag tct atg ggc gaa gat atg ttc tgg gct att aga ggt 672
Leu Asp Lys Lys Ser Met Gly Glu Asp Met Phe Trp Ala Ile Arg Gly
210 215 220
ggt ggc ggt tct tct ttt ggt gtt att ttg gct tgg aag atc aag ttg 720
Gly Gly Gly Ser Ser Phe Gly Val Ile Leu Ala Trp Lys Ile Lys Leu
225 230 235 240
gtt aga atc cca cca aag gtt act gtt ttc acc gtt aga aga aag ttg 768
Val Arg Ile Pro Pro Lys Val Thr Val Phe Thr Val Arg Arg Lys Leu
245 250 255
gaa ggt aac acc atc aac ttg ttg caa aga tgg caa aac gtt tcc cat 816
Glu Gly Asn Thr Ile Asn Leu Leu Gln Arg Trp Gln Asn Val Ser His
260 265 270
aag ttc cca aag gat ttg ttc atg aga gtc ttg atc caa aac atg ggt 864
Lys Phe Pro Lys Asp Leu Phe Met Arg Val Leu Ile Gln Asn Met Gly
275 280 285
ttc ggt aac gaa aag atc gtt caa gtt tca ttc cag ggt ttg ttt ttg 912
Phe Gly Asn Glu Lys Ile Val Gln Val Ser Phe Gln Gly Leu Phe Leu
290 295 300
ggt aga gct tct gaa ttg acc cct ttg ttg aat caa act ttc cca gaa 960
Gly Arg Ala Ser Glu Leu Thr Pro Leu Leu Asn Gln Thr Phe Pro Glu
305 310 315 320
ttc ggt ttg gac cca aaa aac tgt ttc caa gat cca gtt gtt aac tgc 1008
Phe Gly Leu Asp Pro Lys Asn Cys Phe Gln Asp Pro Val Val Asn Cys
325 330 335
aac gaa ttg ccc ttc att aag aaa gaa tgc ttc gaa gtt cca tgg atc 1056
Asn Glu Leu Pro Phe Ile Lys Lys Glu Cys Phe Glu Val Pro Trp Ile
340 345 350
aga tct acc ttg tac ttt gct tct aag agg acc aac aac tcc att gaa 1104
Arg Ser Thr Leu Tyr Phe Ala Ser Lys Arg Thr Asn Asn Ser Ile Glu
355 360 365
ttc ttg gtt aac aac acc gtt tct cag acc aag aac tac caa aaa gct 1152
Phe Leu Val Asn Asn Thr Val Ser Gln Thr Lys Asn Tyr Gln Lys Ala
370 375 380
act tcc gat ttc gtt aag acc cca atg caa gaa gag gtt tgg ggt atg 1200
Thr Ser Asp Phe Val Lys Thr Pro Met Gln Glu Glu Val Trp Gly Met
385 390 395 400
atc aaa gac ttg ttc ttg gaa gaa gag aag ccc atg att att ttg gac 1248
Ile Lys Asp Leu Phe Leu Glu Glu Glu Lys Pro Met Ile Ile Leu Asp
405 410 415
cct tta ggt ggt aag ttg gac gaa att tct gaa tcc gaa ttg cca ttt 1296
Pro Leu Gly Gly Lys Leu Asp Glu Ile Ser Glu Ser Glu Leu Pro Phe
420 425 430
cca cac aga aag ggt aac ttg ttc aac atc cag tac atg gtt aat tgg 1344
Pro His Arg Lys Gly Asn Leu Phe Asn Ile Gln Tyr Met Val Asn Trp
435 440 445
ggt gac aac tcc gaa tct atc tcc tcc aaa aaa atc aaa tgg ctg cgt 1392
Gly Asp Asn Ser Glu Ser Ile Ser Ser Lys Lys Ile Lys Trp Leu Arg
450 455 460
gag ttg tac gag aag atg aag cca ttt ttg tct cat tgt cct aga acc 1440
Glu Leu Tyr Glu Lys Met Lys Pro Phe Leu Ser His Cys Pro Arg Thr
465 470 475 480
gct tac ttg aac tac aga gat ttg gac ttc ggt act aat gat ggt gtc 1488
Ala Tyr Leu Asn Tyr Arg Asp Leu Asp Phe Gly Thr Asn Asp Gly Val
485 490 495
tac aga tac tct aaa gcc aaa gtt tgg ggt gct aag tac ttc aac gaa 1536
Tyr Arg Tyr Ser Lys Ala Lys Val Trp Gly Ala Lys Tyr Phe Asn Glu
500 505 510
aac ttt gaa aga ttg gcc aag gtc aag tct aaa gtt gat cca ggt aat 1584
Asn Phe Glu Arg Leu Ala Lys Val Lys Ser Lys Val Asp Pro Gly Asn
515 520 525
ttc ttc agg tac gaa caa tct att cca cca ttc tct gtt tcc acc 1629
Phe Phe Arg Tyr Glu Gln Ser Ile Pro Pro Phe Ser Val Ser Thr
530 535 540
<210> 67
<211> 543
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 67
Met Gly Arg Leu Gln Leu Phe Ser Cys Leu Leu Val Ser Phe Leu Leu
1 5 10 15
Leu Ile Glu Ala Asn Ala Gln Gln Glu Phe Leu Gln Cys Ile Tyr Ser
20 25 30
His Ser Ser Glu Asn Ile Lys Lys His Ile His Phe Pro Asn Ser Pro
35 40 45
Thr Tyr Ser Ser Leu Leu Glu Tyr Ala Gln Lys Asn Pro Arg Trp Leu
50 55 60
Asn Ser Ser Phe Ala His Pro Ile Phe Ile Ile Ala Pro Lys Asn Glu
65 70 75 80
Ser Glu Ile Gln Pro Ile Ile Leu Cys Ser Gln Lys Phe Gly Leu Gln
85 90 95
Ile Arg Val Lys Ser Gly Gly His Asp Tyr Glu Gly Leu Ser Phe Arg
100 105 110
Ser Val Thr Asn Thr Gln Phe Val Ile Leu Asp Leu Ile Asn Leu Asp
115 120 125
Asp Ile Arg Ile Asp Lys Asn Glu Glu Thr Ala Trp Ile Gln Thr Gly
130 135 140
Val Thr Met Gly Gln Leu Tyr Tyr Glu Ile Ala Lys Lys Ser Lys Ile
145 150 155 160
Leu Ala Phe Pro Gly Gly Leu Tyr Pro Ser Val Gly Ser Gly Gly Leu
165 170 175
Ile Ser Gly Gly Gly Ile Gly Thr Leu Met Arg Lys Phe Gly Leu Ser
180 185 190
Ala Asp Asn Val Ile Asp Ala Arg Val Met Asp Val Asn Gly Arg Ile
195 200 205
Leu Asp Lys Lys Ser Met Gly Glu Asp Met Phe Trp Ala Ile Arg Gly
210 215 220
Gly Gly Gly Ser Ser Phe Gly Val Ile Leu Ala Trp Lys Ile Lys Leu
225 230 235 240
Val Arg Ile Pro Pro Lys Val Thr Val Phe Thr Val Arg Arg Lys Leu
245 250 255
Glu Gly Asn Thr Ile Asn Leu Leu Gln Arg Trp Gln Asn Val Ser His
260 265 270
Lys Phe Pro Lys Asp Leu Phe Met Arg Val Leu Ile Gln Asn Met Gly
275 280 285
Phe Gly Asn Glu Lys Ile Val Gln Val Ser Phe Gln Gly Leu Phe Leu
290 295 300
Gly Arg Ala Ser Glu Leu Thr Pro Leu Leu Asn Gln Thr Phe Pro Glu
305 310 315 320
Phe Gly Leu Asp Pro Lys Asn Cys Phe Gln Asp Pro Val Val Asn Cys
325 330 335
Asn Glu Leu Pro Phe Ile Lys Lys Glu Cys Phe Glu Val Pro Trp Ile
340 345 350
Arg Ser Thr Leu Tyr Phe Ala Ser Lys Arg Thr Asn Asn Ser Ile Glu
355 360 365
Phe Leu Val Asn Asn Thr Val Ser Gln Thr Lys Asn Tyr Gln Lys Ala
370 375 380
Thr Ser Asp Phe Val Lys Thr Pro Met Gln Glu Glu Val Trp Gly Met
385 390 395 400
Ile Lys Asp Leu Phe Leu Glu Glu Glu Lys Pro Met Ile Ile Leu Asp
405 410 415
Pro Leu Gly Gly Lys Leu Asp Glu Ile Ser Glu Ser Glu Leu Pro Phe
420 425 430
Pro His Arg Lys Gly Asn Leu Phe Asn Ile Gln Tyr Met Val Asn Trp
435 440 445
Gly Asp Asn Ser Glu Ser Ile Ser Ser Lys Lys Ile Lys Trp Leu Arg
450 455 460
Glu Leu Tyr Glu Lys Met Lys Pro Phe Leu Ser His Cys Pro Arg Thr
465 470 475 480
Ala Tyr Leu Asn Tyr Arg Asp Leu Asp Phe Gly Thr Asn Asp Gly Val
485 490 495
Tyr Arg Tyr Ser Lys Ala Lys Val Trp Gly Ala Lys Tyr Phe Asn Glu
500 505 510
Asn Phe Glu Arg Leu Ala Lys Val Lys Ser Lys Val Asp Pro Gly Asn
515 520 525
Phe Phe Arg Tyr Glu Gln Ser Ile Pro Pro Phe Ser Val Ser Thr
530 535 540
<210> 68
<211> 1569
<212> DNA
<213> 人工序列
<220>
<223> CBS94-A0A1S3XA06-无信号肽
<220>
<221> CDS
<222> (1)..(1569)
<400> 68
atg gcc caa caa gag ttc ttg caa tgt atc tac tct cac tcc tcc gag 48
Met Ala Gln Gln Glu Phe Leu Gln Cys Ile Tyr Ser His Ser Ser Glu
1 5 10 15
aat att aag aag cac att cat ttc cca aac agc cca act tac tct tcg 96
Asn Ile Lys Lys His Ile His Phe Pro Asn Ser Pro Thr Tyr Ser Ser
20 25 30
ttg ttg gaa tac gct caa aag aat cca aga tgg ctg aat tct tct ttc 144
Leu Leu Glu Tyr Ala Gln Lys Asn Pro Arg Trp Leu Asn Ser Ser Phe
35 40 45
gcc cat cca att ttc att atc gct cca aag aac gaa tcc gaa atc cag 192
Ala His Pro Ile Phe Ile Ile Ala Pro Lys Asn Glu Ser Glu Ile Gln
50 55 60
cca att atc ttg tgc tct caa aag ttc ggc ttg caa atc aga gtt aag 240
Pro Ile Ile Leu Cys Ser Gln Lys Phe Gly Leu Gln Ile Arg Val Lys
65 70 75 80
tct ggt ggt cat gat tac gaa ggt ttg tct ttc aga tct gtc act aac 288
Ser Gly Gly His Asp Tyr Glu Gly Leu Ser Phe Arg Ser Val Thr Asn
85 90 95
acc caa ttc gtt atc ttg gac ttg atc aac ttg gac gat atc aga atc 336
Thr Gln Phe Val Ile Leu Asp Leu Ile Asn Leu Asp Asp Ile Arg Ile
100 105 110
gac aag aac gaa gaa act gct tgg att caa act ggt gtt act atg ggt 384
Asp Lys Asn Glu Glu Thr Ala Trp Ile Gln Thr Gly Val Thr Met Gly
115 120 125
cag ttg tac tac gaa att gcc aag aag tct aag atc ttg gct ttt cca 432
Gln Leu Tyr Tyr Glu Ile Ala Lys Lys Ser Lys Ile Leu Ala Phe Pro
130 135 140
ggt ggt cta tat cca tct gtt ggt tct ggt ggt ttg att tca ggt ggt 480
Gly Gly Leu Tyr Pro Ser Val Gly Ser Gly Gly Leu Ile Ser Gly Gly
145 150 155 160
ggt att ggt act ttg atg aga aag ttt ggt ttg tcc gct gat aac gtc 528
Gly Ile Gly Thr Leu Met Arg Lys Phe Gly Leu Ser Ala Asp Asn Val
165 170 175
att gat gct aga gtt atg gat gtc aac ggt aga atc ttg gac aaa aag 576
Ile Asp Ala Arg Val Met Asp Val Asn Gly Arg Ile Leu Asp Lys Lys
180 185 190
tct atg ggc gaa gat atg ttc tgg gct att aga ggt ggt ggc ggt tct 624
Ser Met Gly Glu Asp Met Phe Trp Ala Ile Arg Gly Gly Gly Gly Ser
195 200 205
tct ttt ggt gtt att ttg gct tgg aag atc aag ttg gtt aga atc cca 672
Ser Phe Gly Val Ile Leu Ala Trp Lys Ile Lys Leu Val Arg Ile Pro
210 215 220
cca aag gtt act gtt ttc acc gtt aga aga aag ttg gaa ggt aac acc 720
Pro Lys Val Thr Val Phe Thr Val Arg Arg Lys Leu Glu Gly Asn Thr
225 230 235 240
atc aac ttg ttg caa aga tgg caa aac gtt tcc cat aag ttc cca aag 768
Ile Asn Leu Leu Gln Arg Trp Gln Asn Val Ser His Lys Phe Pro Lys
245 250 255
gat ttg ttc atg aga gtc ttg atc caa aac atg ggt ttc ggt aac gaa 816
Asp Leu Phe Met Arg Val Leu Ile Gln Asn Met Gly Phe Gly Asn Glu
260 265 270
aag atc gtt caa gtt tca ttc cag ggt ttg ttt ttg ggt aga gct tct 864
Lys Ile Val Gln Val Ser Phe Gln Gly Leu Phe Leu Gly Arg Ala Ser
275 280 285
gaa ttg acc cct ttg ttg aat caa act ttc cca gaa ttc ggt ttg gac 912
Glu Leu Thr Pro Leu Leu Asn Gln Thr Phe Pro Glu Phe Gly Leu Asp
290 295 300
cca aaa aac tgt ttc caa gat cca gtt gtt aac tgc aac gaa ttg ccc 960
Pro Lys Asn Cys Phe Gln Asp Pro Val Val Asn Cys Asn Glu Leu Pro
305 310 315 320
ttc att aag aaa gaa tgc ttc gaa gtt cca tgg atc aga tct acc ttg 1008
Phe Ile Lys Lys Glu Cys Phe Glu Val Pro Trp Ile Arg Ser Thr Leu
325 330 335
tac ttt gct tct aag agg acc aac aac tcc att gaa ttc ttg gtt aac 1056
Tyr Phe Ala Ser Lys Arg Thr Asn Asn Ser Ile Glu Phe Leu Val Asn
340 345 350
aac acc gtt tct cag acc aag aac tac caa aaa gct act tcc gat ttc 1104
Asn Thr Val Ser Gln Thr Lys Asn Tyr Gln Lys Ala Thr Ser Asp Phe
355 360 365
gtt aag acc cca atg caa gaa gag gtt tgg ggt atg atc aaa gac ttg 1152
Val Lys Thr Pro Met Gln Glu Glu Val Trp Gly Met Ile Lys Asp Leu
370 375 380
ttc ttg gaa gaa gag aag ccc atg att att ttg gac cct tta ggt ggt 1200
Phe Leu Glu Glu Glu Lys Pro Met Ile Ile Leu Asp Pro Leu Gly Gly
385 390 395 400
aag ttg gac gaa att tct gaa tcc gaa ttg cca ttt cca cac aga aag 1248
Lys Leu Asp Glu Ile Ser Glu Ser Glu Leu Pro Phe Pro His Arg Lys
405 410 415
ggt aac ttg ttc aac atc cag tac atg gtt aat tgg ggt gac aac tcc 1296
Gly Asn Leu Phe Asn Ile Gln Tyr Met Val Asn Trp Gly Asp Asn Ser
420 425 430
gaa tct atc tcc tcc aaa aaa atc aaa tgg ctg cgt gag ttg tac gag 1344
Glu Ser Ile Ser Ser Lys Lys Ile Lys Trp Leu Arg Glu Leu Tyr Glu
435 440 445
aag atg aag cca ttt ttg tct cat tgt cct aga acc gct tac ttg aac 1392
Lys Met Lys Pro Phe Leu Ser His Cys Pro Arg Thr Ala Tyr Leu Asn
450 455 460
tac aga gat ttg gac ttc ggt act aat gat ggt gtc tac aga tac tct 1440
Tyr Arg Asp Leu Asp Phe Gly Thr Asn Asp Gly Val Tyr Arg Tyr Ser
465 470 475 480
aaa gcc aaa gtt tgg ggt gct aag tac ttc aac gaa aac ttt gaa aga 1488
Lys Ala Lys Val Trp Gly Ala Lys Tyr Phe Asn Glu Asn Phe Glu Arg
485 490 495
ttg gcc aag gtc aag tct aaa gtt gat cca ggt aat ttc ttc agg tac 1536
Leu Ala Lys Val Lys Ser Lys Val Asp Pro Gly Asn Phe Phe Arg Tyr
500 505 510
gaa caa tct att cca cca ttc tct gtt tcc acc 1569
Glu Gln Ser Ile Pro Pro Phe Ser Val Ser Thr
515 520
<210> 69
<211> 523
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 69
Met Ala Gln Gln Glu Phe Leu Gln Cys Ile Tyr Ser His Ser Ser Glu
1 5 10 15
Asn Ile Lys Lys His Ile His Phe Pro Asn Ser Pro Thr Tyr Ser Ser
20 25 30
Leu Leu Glu Tyr Ala Gln Lys Asn Pro Arg Trp Leu Asn Ser Ser Phe
35 40 45
Ala His Pro Ile Phe Ile Ile Ala Pro Lys Asn Glu Ser Glu Ile Gln
50 55 60
Pro Ile Ile Leu Cys Ser Gln Lys Phe Gly Leu Gln Ile Arg Val Lys
65 70 75 80
Ser Gly Gly His Asp Tyr Glu Gly Leu Ser Phe Arg Ser Val Thr Asn
85 90 95
Thr Gln Phe Val Ile Leu Asp Leu Ile Asn Leu Asp Asp Ile Arg Ile
100 105 110
Asp Lys Asn Glu Glu Thr Ala Trp Ile Gln Thr Gly Val Thr Met Gly
115 120 125
Gln Leu Tyr Tyr Glu Ile Ala Lys Lys Ser Lys Ile Leu Ala Phe Pro
130 135 140
Gly Gly Leu Tyr Pro Ser Val Gly Ser Gly Gly Leu Ile Ser Gly Gly
145 150 155 160
Gly Ile Gly Thr Leu Met Arg Lys Phe Gly Leu Ser Ala Asp Asn Val
165 170 175
Ile Asp Ala Arg Val Met Asp Val Asn Gly Arg Ile Leu Asp Lys Lys
180 185 190
Ser Met Gly Glu Asp Met Phe Trp Ala Ile Arg Gly Gly Gly Gly Ser
195 200 205
Ser Phe Gly Val Ile Leu Ala Trp Lys Ile Lys Leu Val Arg Ile Pro
210 215 220
Pro Lys Val Thr Val Phe Thr Val Arg Arg Lys Leu Glu Gly Asn Thr
225 230 235 240
Ile Asn Leu Leu Gln Arg Trp Gln Asn Val Ser His Lys Phe Pro Lys
245 250 255
Asp Leu Phe Met Arg Val Leu Ile Gln Asn Met Gly Phe Gly Asn Glu
260 265 270
Lys Ile Val Gln Val Ser Phe Gln Gly Leu Phe Leu Gly Arg Ala Ser
275 280 285
Glu Leu Thr Pro Leu Leu Asn Gln Thr Phe Pro Glu Phe Gly Leu Asp
290 295 300
Pro Lys Asn Cys Phe Gln Asp Pro Val Val Asn Cys Asn Glu Leu Pro
305 310 315 320
Phe Ile Lys Lys Glu Cys Phe Glu Val Pro Trp Ile Arg Ser Thr Leu
325 330 335
Tyr Phe Ala Ser Lys Arg Thr Asn Asn Ser Ile Glu Phe Leu Val Asn
340 345 350
Asn Thr Val Ser Gln Thr Lys Asn Tyr Gln Lys Ala Thr Ser Asp Phe
355 360 365
Val Lys Thr Pro Met Gln Glu Glu Val Trp Gly Met Ile Lys Asp Leu
370 375 380
Phe Leu Glu Glu Glu Lys Pro Met Ile Ile Leu Asp Pro Leu Gly Gly
385 390 395 400
Lys Leu Asp Glu Ile Ser Glu Ser Glu Leu Pro Phe Pro His Arg Lys
405 410 415
Gly Asn Leu Phe Asn Ile Gln Tyr Met Val Asn Trp Gly Asp Asn Ser
420 425 430
Glu Ser Ile Ser Ser Lys Lys Ile Lys Trp Leu Arg Glu Leu Tyr Glu
435 440 445
Lys Met Lys Pro Phe Leu Ser His Cys Pro Arg Thr Ala Tyr Leu Asn
450 455 460
Tyr Arg Asp Leu Asp Phe Gly Thr Asn Asp Gly Val Tyr Arg Tyr Ser
465 470 475 480
Lys Ala Lys Val Trp Gly Ala Lys Tyr Phe Asn Glu Asn Phe Glu Arg
485 490 495
Leu Ala Lys Val Lys Ser Lys Val Asp Pro Gly Asn Phe Phe Arg Tyr
500 505 510
Glu Gln Ser Ile Pro Pro Phe Ser Val Ser Thr
515 520
<210> 70
<211> 1593
<212> DNA
<213> 人工序列
<220>
<223> CBS94-A0A1S3XA06-无信号肽-strep标签
<220>
<221> CDS
<222> (1)..(1593)
<400> 70
atg gcc caa caa gag ttc ttg caa tgt atc tac tct cac tcc tcc gag 48
Met Ala Gln Gln Glu Phe Leu Gln Cys Ile Tyr Ser His Ser Ser Glu
1 5 10 15
aat att aag aag cac att cat ttc cca aac agc cca act tac tct tcg 96
Asn Ile Lys Lys His Ile His Phe Pro Asn Ser Pro Thr Tyr Ser Ser
20 25 30
ttg ttg gaa tac gct caa aag aat cca aga tgg ctg aat tct tct ttc 144
Leu Leu Glu Tyr Ala Gln Lys Asn Pro Arg Trp Leu Asn Ser Ser Phe
35 40 45
gcc cat cca att ttc att atc gct cca aag aac gaa tcc gaa atc cag 192
Ala His Pro Ile Phe Ile Ile Ala Pro Lys Asn Glu Ser Glu Ile Gln
50 55 60
cca att atc ttg tgc tct caa aag ttc ggc ttg caa atc aga gtt aag 240
Pro Ile Ile Leu Cys Ser Gln Lys Phe Gly Leu Gln Ile Arg Val Lys
65 70 75 80
tct ggt ggt cat gat tac gaa ggt ttg tct ttc aga tct gtc act aac 288
Ser Gly Gly His Asp Tyr Glu Gly Leu Ser Phe Arg Ser Val Thr Asn
85 90 95
acc caa ttc gtt atc ttg gac ttg atc aac ttg gac gat atc aga atc 336
Thr Gln Phe Val Ile Leu Asp Leu Ile Asn Leu Asp Asp Ile Arg Ile
100 105 110
gac aag aac gaa gaa act gct tgg att caa act ggt gtt act atg ggt 384
Asp Lys Asn Glu Glu Thr Ala Trp Ile Gln Thr Gly Val Thr Met Gly
115 120 125
cag ttg tac tac gaa att gcc aag aag tct aag atc ttg gct ttt cca 432
Gln Leu Tyr Tyr Glu Ile Ala Lys Lys Ser Lys Ile Leu Ala Phe Pro
130 135 140
ggt ggt cta tat cca tct gtt ggt tct ggt ggt ttg att tca ggt ggt 480
Gly Gly Leu Tyr Pro Ser Val Gly Ser Gly Gly Leu Ile Ser Gly Gly
145 150 155 160
ggt att ggt act ttg atg aga aag ttt ggt ttg tcc gct gat aac gtc 528
Gly Ile Gly Thr Leu Met Arg Lys Phe Gly Leu Ser Ala Asp Asn Val
165 170 175
att gat gct aga gtt atg gat gtc aac ggt aga atc ttg gac aaa aag 576
Ile Asp Ala Arg Val Met Asp Val Asn Gly Arg Ile Leu Asp Lys Lys
180 185 190
tct atg ggc gaa gat atg ttc tgg gct att aga ggt ggt ggc ggt tct 624
Ser Met Gly Glu Asp Met Phe Trp Ala Ile Arg Gly Gly Gly Gly Ser
195 200 205
tct ttt ggt gtt att ttg gct tgg aag atc aag ttg gtt aga atc cca 672
Ser Phe Gly Val Ile Leu Ala Trp Lys Ile Lys Leu Val Arg Ile Pro
210 215 220
cca aag gtt act gtt ttc acc gtt aga aga aag ttg gaa ggt aac acc 720
Pro Lys Val Thr Val Phe Thr Val Arg Arg Lys Leu Glu Gly Asn Thr
225 230 235 240
atc aac ttg ttg caa aga tgg caa aac gtt tcc cat aag ttc cca aag 768
Ile Asn Leu Leu Gln Arg Trp Gln Asn Val Ser His Lys Phe Pro Lys
245 250 255
gat ttg ttc atg aga gtc ttg atc caa aac atg ggt ttc ggt aac gaa 816
Asp Leu Phe Met Arg Val Leu Ile Gln Asn Met Gly Phe Gly Asn Glu
260 265 270
aag atc gtt caa gtt tca ttc cag ggt ttg ttt ttg ggt aga gct tct 864
Lys Ile Val Gln Val Ser Phe Gln Gly Leu Phe Leu Gly Arg Ala Ser
275 280 285
gaa ttg acc cct ttg ttg aat caa act ttc cca gaa ttc ggt ttg gac 912
Glu Leu Thr Pro Leu Leu Asn Gln Thr Phe Pro Glu Phe Gly Leu Asp
290 295 300
cca aaa aac tgt ttc caa gat cca gtt gtt aac tgc aac gaa ttg ccc 960
Pro Lys Asn Cys Phe Gln Asp Pro Val Val Asn Cys Asn Glu Leu Pro
305 310 315 320
ttc att aag aaa gaa tgc ttc gaa gtt cca tgg atc aga tct acc ttg 1008
Phe Ile Lys Lys Glu Cys Phe Glu Val Pro Trp Ile Arg Ser Thr Leu
325 330 335
tac ttt gct tct aag agg acc aac aac tcc att gaa ttc ttg gtt aac 1056
Tyr Phe Ala Ser Lys Arg Thr Asn Asn Ser Ile Glu Phe Leu Val Asn
340 345 350
aac acc gtt tct cag acc aag aac tac caa aaa gct act tcc gat ttc 1104
Asn Thr Val Ser Gln Thr Lys Asn Tyr Gln Lys Ala Thr Ser Asp Phe
355 360 365
gtt aag acc cca atg caa gaa gag gtt tgg ggt atg atc aaa gac ttg 1152
Val Lys Thr Pro Met Gln Glu Glu Val Trp Gly Met Ile Lys Asp Leu
370 375 380
ttc ttg gaa gaa gag aag ccc atg att att ttg gac cct tta ggt ggt 1200
Phe Leu Glu Glu Glu Lys Pro Met Ile Ile Leu Asp Pro Leu Gly Gly
385 390 395 400
aag ttg gac gaa att tct gaa tcc gaa ttg cca ttt cca cac aga aag 1248
Lys Leu Asp Glu Ile Ser Glu Ser Glu Leu Pro Phe Pro His Arg Lys
405 410 415
ggt aac ttg ttc aac atc cag tac atg gtt aat tgg ggt gac aac tcc 1296
Gly Asn Leu Phe Asn Ile Gln Tyr Met Val Asn Trp Gly Asp Asn Ser
420 425 430
gaa tct atc tcc tcc aaa aaa atc aaa tgg ctg cgt gag ttg tac gag 1344
Glu Ser Ile Ser Ser Lys Lys Ile Lys Trp Leu Arg Glu Leu Tyr Glu
435 440 445
aag atg aag cca ttt ttg tct cat tgt cct aga acc gct tac ttg aac 1392
Lys Met Lys Pro Phe Leu Ser His Cys Pro Arg Thr Ala Tyr Leu Asn
450 455 460
tac aga gat ttg gac ttc ggt act aat gat ggt gtc tac aga tac tct 1440
Tyr Arg Asp Leu Asp Phe Gly Thr Asn Asp Gly Val Tyr Arg Tyr Ser
465 470 475 480
aaa gcc aaa gtt tgg ggt gct aag tac ttc aac gaa aac ttt gaa aga 1488
Lys Ala Lys Val Trp Gly Ala Lys Tyr Phe Asn Glu Asn Phe Glu Arg
485 490 495
ttg gcc aag gtc aag tct aaa gtt gat cca ggt aat ttc ttc agg tac 1536
Leu Ala Lys Val Lys Ser Lys Val Asp Pro Gly Asn Phe Phe Arg Tyr
500 505 510
gaa caa tct att cca cca ttc tct gtt tcc acc tgg tca cat cca caa 1584
Glu Gln Ser Ile Pro Pro Phe Ser Val Ser Thr Trp Ser His Pro Gln
515 520 525
ttt gaa aag 1593
Phe Glu Lys
530
<210> 71
<211> 531
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 71
Met Ala Gln Gln Glu Phe Leu Gln Cys Ile Tyr Ser His Ser Ser Glu
1 5 10 15
Asn Ile Lys Lys His Ile His Phe Pro Asn Ser Pro Thr Tyr Ser Ser
20 25 30
Leu Leu Glu Tyr Ala Gln Lys Asn Pro Arg Trp Leu Asn Ser Ser Phe
35 40 45
Ala His Pro Ile Phe Ile Ile Ala Pro Lys Asn Glu Ser Glu Ile Gln
50 55 60
Pro Ile Ile Leu Cys Ser Gln Lys Phe Gly Leu Gln Ile Arg Val Lys
65 70 75 80
Ser Gly Gly His Asp Tyr Glu Gly Leu Ser Phe Arg Ser Val Thr Asn
85 90 95
Thr Gln Phe Val Ile Leu Asp Leu Ile Asn Leu Asp Asp Ile Arg Ile
100 105 110
Asp Lys Asn Glu Glu Thr Ala Trp Ile Gln Thr Gly Val Thr Met Gly
115 120 125
Gln Leu Tyr Tyr Glu Ile Ala Lys Lys Ser Lys Ile Leu Ala Phe Pro
130 135 140
Gly Gly Leu Tyr Pro Ser Val Gly Ser Gly Gly Leu Ile Ser Gly Gly
145 150 155 160
Gly Ile Gly Thr Leu Met Arg Lys Phe Gly Leu Ser Ala Asp Asn Val
165 170 175
Ile Asp Ala Arg Val Met Asp Val Asn Gly Arg Ile Leu Asp Lys Lys
180 185 190
Ser Met Gly Glu Asp Met Phe Trp Ala Ile Arg Gly Gly Gly Gly Ser
195 200 205
Ser Phe Gly Val Ile Leu Ala Trp Lys Ile Lys Leu Val Arg Ile Pro
210 215 220
Pro Lys Val Thr Val Phe Thr Val Arg Arg Lys Leu Glu Gly Asn Thr
225 230 235 240
Ile Asn Leu Leu Gln Arg Trp Gln Asn Val Ser His Lys Phe Pro Lys
245 250 255
Asp Leu Phe Met Arg Val Leu Ile Gln Asn Met Gly Phe Gly Asn Glu
260 265 270
Lys Ile Val Gln Val Ser Phe Gln Gly Leu Phe Leu Gly Arg Ala Ser
275 280 285
Glu Leu Thr Pro Leu Leu Asn Gln Thr Phe Pro Glu Phe Gly Leu Asp
290 295 300
Pro Lys Asn Cys Phe Gln Asp Pro Val Val Asn Cys Asn Glu Leu Pro
305 310 315 320
Phe Ile Lys Lys Glu Cys Phe Glu Val Pro Trp Ile Arg Ser Thr Leu
325 330 335
Tyr Phe Ala Ser Lys Arg Thr Asn Asn Ser Ile Glu Phe Leu Val Asn
340 345 350
Asn Thr Val Ser Gln Thr Lys Asn Tyr Gln Lys Ala Thr Ser Asp Phe
355 360 365
Val Lys Thr Pro Met Gln Glu Glu Val Trp Gly Met Ile Lys Asp Leu
370 375 380
Phe Leu Glu Glu Glu Lys Pro Met Ile Ile Leu Asp Pro Leu Gly Gly
385 390 395 400
Lys Leu Asp Glu Ile Ser Glu Ser Glu Leu Pro Phe Pro His Arg Lys
405 410 415
Gly Asn Leu Phe Asn Ile Gln Tyr Met Val Asn Trp Gly Asp Asn Ser
420 425 430
Glu Ser Ile Ser Ser Lys Lys Ile Lys Trp Leu Arg Glu Leu Tyr Glu
435 440 445
Lys Met Lys Pro Phe Leu Ser His Cys Pro Arg Thr Ala Tyr Leu Asn
450 455 460
Tyr Arg Asp Leu Asp Phe Gly Thr Asn Asp Gly Val Tyr Arg Tyr Ser
465 470 475 480
Lys Ala Lys Val Trp Gly Ala Lys Tyr Phe Asn Glu Asn Phe Glu Arg
485 490 495
Leu Ala Lys Val Lys Ser Lys Val Asp Pro Gly Asn Phe Phe Arg Tyr
500 505 510
Glu Gln Ser Ile Pro Pro Phe Ser Val Ser Thr Trp Ser His Pro Gln
515 520 525
Phe Glu Lys
530
<210> 72
<211> 1650
<212> DNA
<213> 人工序列
<220>
<223> CBS94-A0A1S3XA06-截断alpha因子-无信号肽-strep标签
<220>
<221> CDS
<222> (1)..(1650)
<400> 72
atg aga ttt cct tcc atc ttc acg gct gtg cta ttt gca gca tcc tcc 48
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser
1 5 10 15
gca ctt gca atg gcc caa caa gag ttc ttg caa tgt atc tac tct cac 96
Ala Leu Ala Met Ala Gln Gln Glu Phe Leu Gln Cys Ile Tyr Ser His
20 25 30
tcc tcc gag aat att aag aag cac att cat ttc cca aac agc cca act 144
Ser Ser Glu Asn Ile Lys Lys His Ile His Phe Pro Asn Ser Pro Thr
35 40 45
tac tct tcg ttg ttg gaa tac gct caa aag aat cca aga tgg ctg aat 192
Tyr Ser Ser Leu Leu Glu Tyr Ala Gln Lys Asn Pro Arg Trp Leu Asn
50 55 60
tct tct ttc gcc cat cca att ttc att atc gct cca aag aac gaa tcc 240
Ser Ser Phe Ala His Pro Ile Phe Ile Ile Ala Pro Lys Asn Glu Ser
65 70 75 80
gaa atc cag cca att atc ttg tgc tct caa aag ttc ggc ttg caa atc 288
Glu Ile Gln Pro Ile Ile Leu Cys Ser Gln Lys Phe Gly Leu Gln Ile
85 90 95
aga gtt aag tct ggt ggt cat gat tac gaa ggt ttg tct ttc aga tct 336
Arg Val Lys Ser Gly Gly His Asp Tyr Glu Gly Leu Ser Phe Arg Ser
100 105 110
gtc act aac acc caa ttc gtt atc ttg gac ttg atc aac ttg gac gat 384
Val Thr Asn Thr Gln Phe Val Ile Leu Asp Leu Ile Asn Leu Asp Asp
115 120 125
atc aga atc gac aag aac gaa gaa act gct tgg att caa act ggt gtt 432
Ile Arg Ile Asp Lys Asn Glu Glu Thr Ala Trp Ile Gln Thr Gly Val
130 135 140
act atg ggt cag ttg tac tac gaa att gcc aag aag tct aag atc ttg 480
Thr Met Gly Gln Leu Tyr Tyr Glu Ile Ala Lys Lys Ser Lys Ile Leu
145 150 155 160
gct ttt cca ggt ggt cta tat cca tct gtt ggt tct ggt ggt ttg att 528
Ala Phe Pro Gly Gly Leu Tyr Pro Ser Val Gly Ser Gly Gly Leu Ile
165 170 175
tca ggt ggt ggt att ggt act ttg atg aga aag ttt ggt ttg tcc gct 576
Ser Gly Gly Gly Ile Gly Thr Leu Met Arg Lys Phe Gly Leu Ser Ala
180 185 190
gat aac gtc att gat gct aga gtt atg gat gtc aac ggt aga atc ttg 624
Asp Asn Val Ile Asp Ala Arg Val Met Asp Val Asn Gly Arg Ile Leu
195 200 205
gac aaa aag tct atg ggc gaa gat atg ttc tgg gct att aga ggt ggt 672
Asp Lys Lys Ser Met Gly Glu Asp Met Phe Trp Ala Ile Arg Gly Gly
210 215 220
ggc ggt tct tct ttt ggt gtt att ttg gct tgg aag atc aag ttg gtt 720
Gly Gly Ser Ser Phe Gly Val Ile Leu Ala Trp Lys Ile Lys Leu Val
225 230 235 240
aga atc cca cca aag gtt act gtt ttc acc gtt aga aga aag ttg gaa 768
Arg Ile Pro Pro Lys Val Thr Val Phe Thr Val Arg Arg Lys Leu Glu
245 250 255
ggt aac acc atc aac ttg ttg caa aga tgg caa aac gtt tcc cat aag 816
Gly Asn Thr Ile Asn Leu Leu Gln Arg Trp Gln Asn Val Ser His Lys
260 265 270
ttc cca aag gat ttg ttc atg aga gtc ttg atc caa aac atg ggt ttc 864
Phe Pro Lys Asp Leu Phe Met Arg Val Leu Ile Gln Asn Met Gly Phe
275 280 285
ggt aac gaa aag atc gtt caa gtt tca ttc cag ggt ttg ttt ttg ggt 912
Gly Asn Glu Lys Ile Val Gln Val Ser Phe Gln Gly Leu Phe Leu Gly
290 295 300
aga gct tct gaa ttg acc cct ttg ttg aat caa act ttc cca gaa ttc 960
Arg Ala Ser Glu Leu Thr Pro Leu Leu Asn Gln Thr Phe Pro Glu Phe
305 310 315 320
ggt ttg gac cca aaa aac tgt ttc caa gat cca gtt gtt aac tgc aac 1008
Gly Leu Asp Pro Lys Asn Cys Phe Gln Asp Pro Val Val Asn Cys Asn
325 330 335
gaa ttg ccc ttc att aag aaa gaa tgc ttc gaa gtt cca tgg atc aga 1056
Glu Leu Pro Phe Ile Lys Lys Glu Cys Phe Glu Val Pro Trp Ile Arg
340 345 350
tct acc ttg tac ttt gct tct aag agg acc aac aac tcc att gaa ttc 1104
Ser Thr Leu Tyr Phe Ala Ser Lys Arg Thr Asn Asn Ser Ile Glu Phe
355 360 365
ttg gtt aac aac acc gtt tct cag acc aag aac tac caa aaa gct act 1152
Leu Val Asn Asn Thr Val Ser Gln Thr Lys Asn Tyr Gln Lys Ala Thr
370 375 380
tcc gat ttc gtt aag acc cca atg caa gaa gag gtt tgg ggt atg atc 1200
Ser Asp Phe Val Lys Thr Pro Met Gln Glu Glu Val Trp Gly Met Ile
385 390 395 400
aaa gac ttg ttc ttg gaa gaa gag aag ccc atg att att ttg gac cct 1248
Lys Asp Leu Phe Leu Glu Glu Glu Lys Pro Met Ile Ile Leu Asp Pro
405 410 415
tta ggt ggt aag ttg gac gaa att tct gaa tcc gaa ttg cca ttt cca 1296
Leu Gly Gly Lys Leu Asp Glu Ile Ser Glu Ser Glu Leu Pro Phe Pro
420 425 430
cac aga aag ggt aac ttg ttc aac atc cag tac atg gtt aat tgg ggt 1344
His Arg Lys Gly Asn Leu Phe Asn Ile Gln Tyr Met Val Asn Trp Gly
435 440 445
gac aac tcc gaa tct atc tcc tcc aaa aaa atc aaa tgg ctg cgt gag 1392
Asp Asn Ser Glu Ser Ile Ser Ser Lys Lys Ile Lys Trp Leu Arg Glu
450 455 460
ttg tac gag aag atg aag cca ttt ttg tct cat tgt cct aga acc gct 1440
Leu Tyr Glu Lys Met Lys Pro Phe Leu Ser His Cys Pro Arg Thr Ala
465 470 475 480
tac ttg aac tac aga gat ttg gac ttc ggt act aat gat ggt gtc tac 1488
Tyr Leu Asn Tyr Arg Asp Leu Asp Phe Gly Thr Asn Asp Gly Val Tyr
485 490 495
aga tac tct aaa gcc aaa gtt tgg ggt gct aag tac ttc aac gaa aac 1536
Arg Tyr Ser Lys Ala Lys Val Trp Gly Ala Lys Tyr Phe Asn Glu Asn
500 505 510
ttt gaa aga ttg gcc aag gtc aag tct aaa gtt gat cca ggt aat ttc 1584
Phe Glu Arg Leu Ala Lys Val Lys Ser Lys Val Asp Pro Gly Asn Phe
515 520 525
ttc agg tac gaa caa tct att cca cca ttc tct gtt tcc acc tgg tca 1632
Phe Arg Tyr Glu Gln Ser Ile Pro Pro Phe Ser Val Ser Thr Trp Ser
530 535 540
cat cca caa ttt gaa aag 1650
His Pro Gln Phe Glu Lys
545 550
<210> 73
<211> 550
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 73
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser
1 5 10 15
Ala Leu Ala Met Ala Gln Gln Glu Phe Leu Gln Cys Ile Tyr Ser His
20 25 30
Ser Ser Glu Asn Ile Lys Lys His Ile His Phe Pro Asn Ser Pro Thr
35 40 45
Tyr Ser Ser Leu Leu Glu Tyr Ala Gln Lys Asn Pro Arg Trp Leu Asn
50 55 60
Ser Ser Phe Ala His Pro Ile Phe Ile Ile Ala Pro Lys Asn Glu Ser
65 70 75 80
Glu Ile Gln Pro Ile Ile Leu Cys Ser Gln Lys Phe Gly Leu Gln Ile
85 90 95
Arg Val Lys Ser Gly Gly His Asp Tyr Glu Gly Leu Ser Phe Arg Ser
100 105 110
Val Thr Asn Thr Gln Phe Val Ile Leu Asp Leu Ile Asn Leu Asp Asp
115 120 125
Ile Arg Ile Asp Lys Asn Glu Glu Thr Ala Trp Ile Gln Thr Gly Val
130 135 140
Thr Met Gly Gln Leu Tyr Tyr Glu Ile Ala Lys Lys Ser Lys Ile Leu
145 150 155 160
Ala Phe Pro Gly Gly Leu Tyr Pro Ser Val Gly Ser Gly Gly Leu Ile
165 170 175
Ser Gly Gly Gly Ile Gly Thr Leu Met Arg Lys Phe Gly Leu Ser Ala
180 185 190
Asp Asn Val Ile Asp Ala Arg Val Met Asp Val Asn Gly Arg Ile Leu
195 200 205
Asp Lys Lys Ser Met Gly Glu Asp Met Phe Trp Ala Ile Arg Gly Gly
210 215 220
Gly Gly Ser Ser Phe Gly Val Ile Leu Ala Trp Lys Ile Lys Leu Val
225 230 235 240
Arg Ile Pro Pro Lys Val Thr Val Phe Thr Val Arg Arg Lys Leu Glu
245 250 255
Gly Asn Thr Ile Asn Leu Leu Gln Arg Trp Gln Asn Val Ser His Lys
260 265 270
Phe Pro Lys Asp Leu Phe Met Arg Val Leu Ile Gln Asn Met Gly Phe
275 280 285
Gly Asn Glu Lys Ile Val Gln Val Ser Phe Gln Gly Leu Phe Leu Gly
290 295 300
Arg Ala Ser Glu Leu Thr Pro Leu Leu Asn Gln Thr Phe Pro Glu Phe
305 310 315 320
Gly Leu Asp Pro Lys Asn Cys Phe Gln Asp Pro Val Val Asn Cys Asn
325 330 335
Glu Leu Pro Phe Ile Lys Lys Glu Cys Phe Glu Val Pro Trp Ile Arg
340 345 350
Ser Thr Leu Tyr Phe Ala Ser Lys Arg Thr Asn Asn Ser Ile Glu Phe
355 360 365
Leu Val Asn Asn Thr Val Ser Gln Thr Lys Asn Tyr Gln Lys Ala Thr
370 375 380
Ser Asp Phe Val Lys Thr Pro Met Gln Glu Glu Val Trp Gly Met Ile
385 390 395 400
Lys Asp Leu Phe Leu Glu Glu Glu Lys Pro Met Ile Ile Leu Asp Pro
405 410 415
Leu Gly Gly Lys Leu Asp Glu Ile Ser Glu Ser Glu Leu Pro Phe Pro
420 425 430
His Arg Lys Gly Asn Leu Phe Asn Ile Gln Tyr Met Val Asn Trp Gly
435 440 445
Asp Asn Ser Glu Ser Ile Ser Ser Lys Lys Ile Lys Trp Leu Arg Glu
450 455 460
Leu Tyr Glu Lys Met Lys Pro Phe Leu Ser His Cys Pro Arg Thr Ala
465 470 475 480
Tyr Leu Asn Tyr Arg Asp Leu Asp Phe Gly Thr Asn Asp Gly Val Tyr
485 490 495
Arg Tyr Ser Lys Ala Lys Val Trp Gly Ala Lys Tyr Phe Asn Glu Asn
500 505 510
Phe Glu Arg Leu Ala Lys Val Lys Ser Lys Val Asp Pro Gly Asn Phe
515 520 525
Phe Arg Tyr Glu Gln Ser Ile Pro Pro Phe Ser Val Ser Thr Trp Ser
530 535 540
His Pro Gln Phe Glu Lys
545 550
<210> 74
<211> 1860
<212> DNA
<213> 人工序列
<220>
<223> CBS94-A0A1S3XA06-全长alpha因子-无信号肽-strep标签
<220>
<221> CDS
<222> (1)..(1860)
<400> 74
atg aga ttt cct tca att ttt act gct gtt tta ttc gca gca tcc tcc 48
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser
1 5 10 15
gca tta gct gct cca gtc aac act aca aca gaa gat gaa acg gca caa 96
Ala Leu Ala Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln
20 25 30
att ccg gct gaa gct gtc atc ggt tac tca gat tta gaa ggg gat ttc 144
Ile Pro Ala Glu Ala Val Ile Gly Tyr Ser Asp Leu Glu Gly Asp Phe
35 40 45
gat gtt gct gtt ttg cca ttt tcc aac agc aca aat aac ggg tta ttg 192
Asp Val Ala Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu
50 55 60
ttt ata aat act act att gcc agc att gct gct aaa gaa gaa ggg gta 240
Phe Ile Asn Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val
65 70 75 80
tct ctc gag aaa aga gag gct gaa gct atg gcc caa caa gag ttc ttg 288
Ser Leu Glu Lys Arg Glu Ala Glu Ala Met Ala Gln Gln Glu Phe Leu
85 90 95
caa tgt atc tac tct cac tcc tcc gag aat att aag aag cac att cat 336
Gln Cys Ile Tyr Ser His Ser Ser Glu Asn Ile Lys Lys His Ile His
100 105 110
ttc cca aac agc cca act tac tct tcg ttg ttg gaa tac gct caa aag 384
Phe Pro Asn Ser Pro Thr Tyr Ser Ser Leu Leu Glu Tyr Ala Gln Lys
115 120 125
aat cca aga tgg ctg aat tct tct ttc gcc cat cca att ttc att atc 432
Asn Pro Arg Trp Leu Asn Ser Ser Phe Ala His Pro Ile Phe Ile Ile
130 135 140
gct cca aag aac gaa tcc gaa atc cag cca att atc ttg tgc tct caa 480
Ala Pro Lys Asn Glu Ser Glu Ile Gln Pro Ile Ile Leu Cys Ser Gln
145 150 155 160
aag ttc ggc ttg caa atc aga gtt aag tct ggt ggt cat gat tac gaa 528
Lys Phe Gly Leu Gln Ile Arg Val Lys Ser Gly Gly His Asp Tyr Glu
165 170 175
ggt ttg tct ttc aga tct gtc act aac acc caa ttc gtt atc ttg gac 576
Gly Leu Ser Phe Arg Ser Val Thr Asn Thr Gln Phe Val Ile Leu Asp
180 185 190
ttg atc aac ttg gac gat atc aga atc gac aag aac gaa gaa act gct 624
Leu Ile Asn Leu Asp Asp Ile Arg Ile Asp Lys Asn Glu Glu Thr Ala
195 200 205
tgg att caa act ggt gtt act atg ggt cag ttg tac tac gaa att gcc 672
Trp Ile Gln Thr Gly Val Thr Met Gly Gln Leu Tyr Tyr Glu Ile Ala
210 215 220
aag aag tct aag atc ttg gct ttt cca ggt ggt cta tat cca tct gtt 720
Lys Lys Ser Lys Ile Leu Ala Phe Pro Gly Gly Leu Tyr Pro Ser Val
225 230 235 240
ggt tct ggt ggt ttg att tca ggt ggt ggt att ggt act ttg atg aga 768
Gly Ser Gly Gly Leu Ile Ser Gly Gly Gly Ile Gly Thr Leu Met Arg
245 250 255
aag ttt ggt ttg tcc gct gat aac gtc att gat gct aga gtt atg gat 816
Lys Phe Gly Leu Ser Ala Asp Asn Val Ile Asp Ala Arg Val Met Asp
260 265 270
gtc aac ggt aga atc ttg gac aaa aag tct atg ggc gaa gat atg ttc 864
Val Asn Gly Arg Ile Leu Asp Lys Lys Ser Met Gly Glu Asp Met Phe
275 280 285
tgg gct att aga ggt ggt ggc ggt tct tct ttt ggt gtt att ttg gct 912
Trp Ala Ile Arg Gly Gly Gly Gly Ser Ser Phe Gly Val Ile Leu Ala
290 295 300
tgg aag atc aag ttg gtt aga atc cca cca aag gtt act gtt ttc acc 960
Trp Lys Ile Lys Leu Val Arg Ile Pro Pro Lys Val Thr Val Phe Thr
305 310 315 320
gtt aga aga aag ttg gaa ggt aac acc atc aac ttg ttg caa aga tgg 1008
Val Arg Arg Lys Leu Glu Gly Asn Thr Ile Asn Leu Leu Gln Arg Trp
325 330 335
caa aac gtt tcc cat aag ttc cca aag gat ttg ttc atg aga gtc ttg 1056
Gln Asn Val Ser His Lys Phe Pro Lys Asp Leu Phe Met Arg Val Leu
340 345 350
atc caa aac atg ggt ttc ggt aac gaa aag atc gtt caa gtt tca ttc 1104
Ile Gln Asn Met Gly Phe Gly Asn Glu Lys Ile Val Gln Val Ser Phe
355 360 365
cag ggt ttg ttt ttg ggt aga gct tct gaa ttg acc cct ttg ttg aat 1152
Gln Gly Leu Phe Leu Gly Arg Ala Ser Glu Leu Thr Pro Leu Leu Asn
370 375 380
caa act ttc cca gaa ttc ggt ttg gac cca aaa aac tgt ttc caa gat 1200
Gln Thr Phe Pro Glu Phe Gly Leu Asp Pro Lys Asn Cys Phe Gln Asp
385 390 395 400
cca gtt gtt aac tgc aac gaa ttg ccc ttc att aag aaa gaa tgc ttc 1248
Pro Val Val Asn Cys Asn Glu Leu Pro Phe Ile Lys Lys Glu Cys Phe
405 410 415
gaa gtt cca tgg atc aga tct acc ttg tac ttt gct tct aag agg acc 1296
Glu Val Pro Trp Ile Arg Ser Thr Leu Tyr Phe Ala Ser Lys Arg Thr
420 425 430
aac aac tcc att gaa ttc ttg gtt aac aac acc gtt tct cag acc aag 1344
Asn Asn Ser Ile Glu Phe Leu Val Asn Asn Thr Val Ser Gln Thr Lys
435 440 445
aac tac caa aaa gct act tcc gat ttc gtt aag acc cca atg caa gaa 1392
Asn Tyr Gln Lys Ala Thr Ser Asp Phe Val Lys Thr Pro Met Gln Glu
450 455 460
gag gtt tgg ggt atg atc aaa gac ttg ttc ttg gaa gaa gag aag ccc 1440
Glu Val Trp Gly Met Ile Lys Asp Leu Phe Leu Glu Glu Glu Lys Pro
465 470 475 480
atg att att ttg gac cct tta ggt ggt aag ttg gac gaa att tct gaa 1488
Met Ile Ile Leu Asp Pro Leu Gly Gly Lys Leu Asp Glu Ile Ser Glu
485 490 495
tcc gaa ttg cca ttt cca cac aga aag ggt aac ttg ttc aac atc cag 1536
Ser Glu Leu Pro Phe Pro His Arg Lys Gly Asn Leu Phe Asn Ile Gln
500 505 510
tac atg gtt aat tgg ggt gac aac tcc gaa tct atc tcc tcc aaa aaa 1584
Tyr Met Val Asn Trp Gly Asp Asn Ser Glu Ser Ile Ser Ser Lys Lys
515 520 525
atc aaa tgg ctg cgt gag ttg tac gag aag atg aag cca ttt ttg tct 1632
Ile Lys Trp Leu Arg Glu Leu Tyr Glu Lys Met Lys Pro Phe Leu Ser
530 535 540
cat tgt cct aga acc gct tac ttg aac tac aga gat ttg gac ttc ggt 1680
His Cys Pro Arg Thr Ala Tyr Leu Asn Tyr Arg Asp Leu Asp Phe Gly
545 550 555 560
act aat gat ggt gtc tac aga tac tct aaa gcc aaa gtt tgg ggt gct 1728
Thr Asn Asp Gly Val Tyr Arg Tyr Ser Lys Ala Lys Val Trp Gly Ala
565 570 575
aag tac ttc aac gaa aac ttt gaa aga ttg gcc aag gtc aag tct aaa 1776
Lys Tyr Phe Asn Glu Asn Phe Glu Arg Leu Ala Lys Val Lys Ser Lys
580 585 590
gtt gat cca ggt aat ttc ttc agg tac gaa caa tct att cca cca ttc 1824
Val Asp Pro Gly Asn Phe Phe Arg Tyr Glu Gln Ser Ile Pro Pro Phe
595 600 605
tct gtt tcc acc tgg tca cat cca caa ttt gaa aag 1860
Ser Val Ser Thr Trp Ser His Pro Gln Phe Glu Lys
610 615 620
<210> 75
<211> 620
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 75
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser
1 5 10 15
Ala Leu Ala Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln
20 25 30
Ile Pro Ala Glu Ala Val Ile Gly Tyr Ser Asp Leu Glu Gly Asp Phe
35 40 45
Asp Val Ala Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu
50 55 60
Phe Ile Asn Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val
65 70 75 80
Ser Leu Glu Lys Arg Glu Ala Glu Ala Met Ala Gln Gln Glu Phe Leu
85 90 95
Gln Cys Ile Tyr Ser His Ser Ser Glu Asn Ile Lys Lys His Ile His
100 105 110
Phe Pro Asn Ser Pro Thr Tyr Ser Ser Leu Leu Glu Tyr Ala Gln Lys
115 120 125
Asn Pro Arg Trp Leu Asn Ser Ser Phe Ala His Pro Ile Phe Ile Ile
130 135 140
Ala Pro Lys Asn Glu Ser Glu Ile Gln Pro Ile Ile Leu Cys Ser Gln
145 150 155 160
Lys Phe Gly Leu Gln Ile Arg Val Lys Ser Gly Gly His Asp Tyr Glu
165 170 175
Gly Leu Ser Phe Arg Ser Val Thr Asn Thr Gln Phe Val Ile Leu Asp
180 185 190
Leu Ile Asn Leu Asp Asp Ile Arg Ile Asp Lys Asn Glu Glu Thr Ala
195 200 205
Trp Ile Gln Thr Gly Val Thr Met Gly Gln Leu Tyr Tyr Glu Ile Ala
210 215 220
Lys Lys Ser Lys Ile Leu Ala Phe Pro Gly Gly Leu Tyr Pro Ser Val
225 230 235 240
Gly Ser Gly Gly Leu Ile Ser Gly Gly Gly Ile Gly Thr Leu Met Arg
245 250 255
Lys Phe Gly Leu Ser Ala Asp Asn Val Ile Asp Ala Arg Val Met Asp
260 265 270
Val Asn Gly Arg Ile Leu Asp Lys Lys Ser Met Gly Glu Asp Met Phe
275 280 285
Trp Ala Ile Arg Gly Gly Gly Gly Ser Ser Phe Gly Val Ile Leu Ala
290 295 300
Trp Lys Ile Lys Leu Val Arg Ile Pro Pro Lys Val Thr Val Phe Thr
305 310 315 320
Val Arg Arg Lys Leu Glu Gly Asn Thr Ile Asn Leu Leu Gln Arg Trp
325 330 335
Gln Asn Val Ser His Lys Phe Pro Lys Asp Leu Phe Met Arg Val Leu
340 345 350
Ile Gln Asn Met Gly Phe Gly Asn Glu Lys Ile Val Gln Val Ser Phe
355 360 365
Gln Gly Leu Phe Leu Gly Arg Ala Ser Glu Leu Thr Pro Leu Leu Asn
370 375 380
Gln Thr Phe Pro Glu Phe Gly Leu Asp Pro Lys Asn Cys Phe Gln Asp
385 390 395 400
Pro Val Val Asn Cys Asn Glu Leu Pro Phe Ile Lys Lys Glu Cys Phe
405 410 415
Glu Val Pro Trp Ile Arg Ser Thr Leu Tyr Phe Ala Ser Lys Arg Thr
420 425 430
Asn Asn Ser Ile Glu Phe Leu Val Asn Asn Thr Val Ser Gln Thr Lys
435 440 445
Asn Tyr Gln Lys Ala Thr Ser Asp Phe Val Lys Thr Pro Met Gln Glu
450 455 460
Glu Val Trp Gly Met Ile Lys Asp Leu Phe Leu Glu Glu Glu Lys Pro
465 470 475 480
Met Ile Ile Leu Asp Pro Leu Gly Gly Lys Leu Asp Glu Ile Ser Glu
485 490 495
Ser Glu Leu Pro Phe Pro His Arg Lys Gly Asn Leu Phe Asn Ile Gln
500 505 510
Tyr Met Val Asn Trp Gly Asp Asn Ser Glu Ser Ile Ser Ser Lys Lys
515 520 525
Ile Lys Trp Leu Arg Glu Leu Tyr Glu Lys Met Lys Pro Phe Leu Ser
530 535 540
His Cys Pro Arg Thr Ala Tyr Leu Asn Tyr Arg Asp Leu Asp Phe Gly
545 550 555 560
Thr Asn Asp Gly Val Tyr Arg Tyr Ser Lys Ala Lys Val Trp Gly Ala
565 570 575
Lys Tyr Phe Asn Glu Asn Phe Glu Arg Leu Ala Lys Val Lys Ser Lys
580 585 590
Val Asp Pro Gly Asn Phe Phe Arg Tyr Glu Gln Ser Ile Pro Pro Phe
595 600 605
Ser Val Ser Thr Trp Ser His Pro Gln Phe Glu Lys
610 615 620
<210> 76
<211> 1608
<212> DNA
<213> 人工序列
<220>
<223> CBS99-A0A1S3BDA8
<220>
<221> CDS
<222> (1)..(1608)
<400> 76
atg aag ttc tcc tct acc ttg att cca ctg tct ttg gtt ttt atc gtt 48
Met Lys Phe Ser Ser Thr Leu Ile Pro Leu Ser Leu Val Phe Ile Val
1 5 10 15
gtc tcc tct gct tct ttg tgt gct tct gct tct cca caa aag tac caa 96
Val Ser Ser Ala Ser Leu Cys Ala Ser Ala Ser Pro Gln Lys Tyr Gln
20 25 30
aat ttc ttg cag tgc ttg tcc gaa cac tct tct aaa tct tac cca atc 144
Asn Phe Leu Gln Cys Leu Ser Glu His Ser Ser Lys Ser Tyr Pro Ile
35 40 45
tcc aag gtt gtc tat acc cca atc aat tcc tct tac tcc tct gtt ttg 192
Ser Lys Val Val Tyr Thr Pro Ile Asn Ser Ser Tyr Ser Ser Val Leu
50 55 60
gac ttc tcc att agg aac ttg ttg ttc tct aaa cca gaa acg cca aag 240
Asp Phe Ser Ile Arg Asn Leu Leu Phe Ser Lys Pro Glu Thr Pro Lys
65 70 75 80
cct ttg ttg att att act cca tct cac gtg tcc cat att caa gct gct 288
Pro Leu Leu Ile Ile Thr Pro Ser His Val Ser His Ile Gln Ala Ala
85 90 95
gtt att tgt tct aag tcc cat ggc ttg caa atc aga act aga tct ggt 336
Val Ile Cys Ser Lys Ser His Gly Leu Gln Ile Arg Thr Arg Ser Gly
100 105 110
ggt cat gat ttc gaa ggt ttg tct tat gtt gct tac aga cca ttc atc 384
Gly His Asp Phe Glu Gly Leu Ser Tyr Val Ala Tyr Arg Pro Phe Ile
115 120 125
gtt gtc gac ttg att aac ttg aga tcc gtt aag gtt gaa gtc gaa aac 432
Val Val Asp Leu Ile Asn Leu Arg Ser Val Lys Val Glu Val Glu Asn
130 135 140
aat act gct tgg gtt gaa tct ggt gct act ttg ggt gag ttg tat tac 480
Asn Thr Ala Trp Val Glu Ser Gly Ala Thr Leu Gly Glu Leu Tyr Tyr
145 150 155 160
aga att ggt gaa aag tct agg acc ttg gct ttt cca gct ggt gtt tgt 528
Arg Ile Gly Glu Lys Ser Arg Thr Leu Ala Phe Pro Ala Gly Val Cys
165 170 175
cca act gtt ggt ata ggt ggt cat ttt tct ggt ggt ggt tat ggt ttg 576
Pro Thr Val Gly Ile Gly Gly His Phe Ser Gly Gly Gly Tyr Gly Leu
180 185 190
atg ttg aga aaa ttt ggt ttg gcc gct gat aac gtc att gat gct tat 624
Met Leu Arg Lys Phe Gly Leu Ala Ala Asp Asn Val Ile Asp Ala Tyr
195 200 205
ttg gtt gat gcc aac ggt aag gtt ttg gac aaa gaa tct atg ggc gaa 672
Leu Val Asp Ala Asn Gly Lys Val Leu Asp Lys Glu Ser Met Gly Glu
210 215 220
gat ttg ttc tgg gct att aga ggt ggt ggt ggc ggt tct ttt ggt ata 720
Asp Leu Phe Trp Ala Ile Arg Gly Gly Gly Gly Gly Ser Phe Gly Ile
225 230 235 240
gtt gtt gct tgg aag atc aag ttg gtt cca gtt cca gaa act gtt act 768
Val Val Ala Trp Lys Ile Lys Leu Val Pro Val Pro Glu Thr Val Thr
245 250 255
att tgc tct acc gac aga aac ttg gaa gaa gat gcc att aag ttg atc 816
Ile Cys Ser Thr Asp Arg Asn Leu Glu Glu Asp Ala Ile Lys Leu Ile
260 265 270
cac aga tgg caa tac gtt ggt aac aaa ttg gac gag aat atc tac ttg 864
His Arg Trp Gln Tyr Val Gly Asn Lys Leu Asp Glu Asn Ile Tyr Leu
275 280 285
ggt atc att ttg act ggt ggt aac gct tct act caa gct ggt att act 912
Gly Ile Ile Leu Thr Gly Gly Asn Ala Ser Thr Gln Ala Gly Ile Thr
290 295 300
aat cca act gcc aga ttc ttc agc ttg ttt ttg ggt aga gct gat gaa 960
Asn Pro Thr Ala Arg Phe Phe Ser Leu Phe Leu Gly Arg Ala Asp Glu
305 310 315 320
ttg gtt act acc ttg tct act atc ttc cca gaa ttg ggt ttg atc aag 1008
Leu Val Thr Thr Leu Ser Thr Ile Phe Pro Glu Leu Gly Leu Ile Lys
325 330 335
caa gat tgt gtt gaa gct tct tgg gtc gaa tcc act ttg att att cca 1056
Gln Asp Cys Val Glu Ala Ser Trp Val Glu Ser Thr Leu Ile Ile Pro
340 345 350
att ggt gtt cag cca atc gaa acc ttg gaa cca tta ttg aac aga act 1104
Ile Gly Val Gln Pro Ile Glu Thr Leu Glu Pro Leu Leu Asn Arg Thr
355 360 365
ttg acc acc ttg gac tcc act aag att aag tct gat tac atc acc gaa 1152
Leu Thr Thr Leu Asp Ser Thr Lys Ile Lys Ser Asp Tyr Ile Thr Glu
370 375 380
cct att cca gtt gct gct att aag gat atc tgg cag aga ttg aaa acc 1200
Pro Ile Pro Val Ala Ala Ile Lys Asp Ile Trp Gln Arg Leu Lys Thr
385 390 395 400
caa gac att gaa acc tct cag ttg gtt ttt gtt cca tac ggt ggt aga 1248
Gln Asp Ile Glu Thr Ser Gln Leu Val Phe Val Pro Tyr Gly Gly Arg
405 410 415
atg tcc caa atc tct gaa tct gaa act cca ttc tct cat aga gcc aga 1296
Met Ser Gln Ile Ser Glu Ser Glu Thr Pro Phe Ser His Arg Ala Arg
420 425 430
tac ttg ttc aag atc ggt tat gtt gta ggt tgg aag gac caa tct ttg 1344
Tyr Leu Phe Lys Ile Gly Tyr Val Val Gly Trp Lys Asp Gln Ser Leu
435 440 445
aag gct aag aag aga cat atc tcc tgg ttg aga gaa gtt tat gag tac 1392
Lys Ala Lys Lys Arg His Ile Ser Trp Leu Arg Glu Val Tyr Glu Tyr
450 455 460
atg gct cca ttc gtt tct aaa tca cca aga gct gct tac acc aat tac 1440
Met Ala Pro Phe Val Ser Lys Ser Pro Arg Ala Ala Tyr Thr Asn Tyr
465 470 475 480
aga gac ttg gat att ggc tcc aac aac aaa tac ggt aag acc tct tac 1488
Arg Asp Leu Asp Ile Gly Ser Asn Asn Lys Tyr Gly Lys Thr Ser Tyr
485 490 495
aag aga gct tct gtt tgg ggt ttg aag tat ttc ggt aac aac ttc gat 1536
Lys Arg Ala Ser Val Trp Gly Leu Lys Tyr Phe Gly Asn Asn Phe Asp
500 505 510
aga ctg gtt tac gtt aag acc aag gtt gat cca tac gat ttc ttc aga 1584
Arg Leu Val Tyr Val Lys Thr Lys Val Asp Pro Tyr Asp Phe Phe Arg
515 520 525
cac gaa caa tct att ccc acc ttg 1608
His Glu Gln Ser Ile Pro Thr Leu
530 535
<210> 77
<211> 536
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 77
Met Lys Phe Ser Ser Thr Leu Ile Pro Leu Ser Leu Val Phe Ile Val
1 5 10 15
Val Ser Ser Ala Ser Leu Cys Ala Ser Ala Ser Pro Gln Lys Tyr Gln
20 25 30
Asn Phe Leu Gln Cys Leu Ser Glu His Ser Ser Lys Ser Tyr Pro Ile
35 40 45
Ser Lys Val Val Tyr Thr Pro Ile Asn Ser Ser Tyr Ser Ser Val Leu
50 55 60
Asp Phe Ser Ile Arg Asn Leu Leu Phe Ser Lys Pro Glu Thr Pro Lys
65 70 75 80
Pro Leu Leu Ile Ile Thr Pro Ser His Val Ser His Ile Gln Ala Ala
85 90 95
Val Ile Cys Ser Lys Ser His Gly Leu Gln Ile Arg Thr Arg Ser Gly
100 105 110
Gly His Asp Phe Glu Gly Leu Ser Tyr Val Ala Tyr Arg Pro Phe Ile
115 120 125
Val Val Asp Leu Ile Asn Leu Arg Ser Val Lys Val Glu Val Glu Asn
130 135 140
Asn Thr Ala Trp Val Glu Ser Gly Ala Thr Leu Gly Glu Leu Tyr Tyr
145 150 155 160
Arg Ile Gly Glu Lys Ser Arg Thr Leu Ala Phe Pro Ala Gly Val Cys
165 170 175
Pro Thr Val Gly Ile Gly Gly His Phe Ser Gly Gly Gly Tyr Gly Leu
180 185 190
Met Leu Arg Lys Phe Gly Leu Ala Ala Asp Asn Val Ile Asp Ala Tyr
195 200 205
Leu Val Asp Ala Asn Gly Lys Val Leu Asp Lys Glu Ser Met Gly Glu
210 215 220
Asp Leu Phe Trp Ala Ile Arg Gly Gly Gly Gly Gly Ser Phe Gly Ile
225 230 235 240
Val Val Ala Trp Lys Ile Lys Leu Val Pro Val Pro Glu Thr Val Thr
245 250 255
Ile Cys Ser Thr Asp Arg Asn Leu Glu Glu Asp Ala Ile Lys Leu Ile
260 265 270
His Arg Trp Gln Tyr Val Gly Asn Lys Leu Asp Glu Asn Ile Tyr Leu
275 280 285
Gly Ile Ile Leu Thr Gly Gly Asn Ala Ser Thr Gln Ala Gly Ile Thr
290 295 300
Asn Pro Thr Ala Arg Phe Phe Ser Leu Phe Leu Gly Arg Ala Asp Glu
305 310 315 320
Leu Val Thr Thr Leu Ser Thr Ile Phe Pro Glu Leu Gly Leu Ile Lys
325 330 335
Gln Asp Cys Val Glu Ala Ser Trp Val Glu Ser Thr Leu Ile Ile Pro
340 345 350
Ile Gly Val Gln Pro Ile Glu Thr Leu Glu Pro Leu Leu Asn Arg Thr
355 360 365
Leu Thr Thr Leu Asp Ser Thr Lys Ile Lys Ser Asp Tyr Ile Thr Glu
370 375 380
Pro Ile Pro Val Ala Ala Ile Lys Asp Ile Trp Gln Arg Leu Lys Thr
385 390 395 400
Gln Asp Ile Glu Thr Ser Gln Leu Val Phe Val Pro Tyr Gly Gly Arg
405 410 415
Met Ser Gln Ile Ser Glu Ser Glu Thr Pro Phe Ser His Arg Ala Arg
420 425 430
Tyr Leu Phe Lys Ile Gly Tyr Val Val Gly Trp Lys Asp Gln Ser Leu
435 440 445
Lys Ala Lys Lys Arg His Ile Ser Trp Leu Arg Glu Val Tyr Glu Tyr
450 455 460
Met Ala Pro Phe Val Ser Lys Ser Pro Arg Ala Ala Tyr Thr Asn Tyr
465 470 475 480
Arg Asp Leu Asp Ile Gly Ser Asn Asn Lys Tyr Gly Lys Thr Ser Tyr
485 490 495
Lys Arg Ala Ser Val Trp Gly Leu Lys Tyr Phe Gly Asn Asn Phe Asp
500 505 510
Arg Leu Val Tyr Val Lys Thr Lys Val Asp Pro Tyr Asp Phe Phe Arg
515 520 525
His Glu Gln Ser Ile Pro Thr Leu
530 535
<210> 78
<211> 1536
<212> DNA
<213> 人工序列
<220>
<223> CBS99-A0A1S3BDA8-无信号肽
<220>
<221> CDS
<222> (1)..(1536)
<400> 78
atg gct tct cca caa aag tac caa aat ttc ttg cag tgc ttg tcc gaa 48
Met Ala Ser Pro Gln Lys Tyr Gln Asn Phe Leu Gln Cys Leu Ser Glu
1 5 10 15
cac tct tct aaa tct tac cca atc tcc aag gtt gtc tat acc cca atc 96
His Ser Ser Lys Ser Tyr Pro Ile Ser Lys Val Val Tyr Thr Pro Ile
20 25 30
aat tcc tct tac tcc tct gtt ttg gac ttc tcc att agg aac ttg ttg 144
Asn Ser Ser Tyr Ser Ser Val Leu Asp Phe Ser Ile Arg Asn Leu Leu
35 40 45
ttc tct aaa cca gaa acg cca aag cct ttg ttg att att act cca tct 192
Phe Ser Lys Pro Glu Thr Pro Lys Pro Leu Leu Ile Ile Thr Pro Ser
50 55 60
cac gtg tcc cat att caa gct gct gtt att tgt tct aag tcc cat ggc 240
His Val Ser His Ile Gln Ala Ala Val Ile Cys Ser Lys Ser His Gly
65 70 75 80
ttg caa atc aga act aga tct ggt ggt cat gat ttc gaa ggt ttg tct 288
Leu Gln Ile Arg Thr Arg Ser Gly Gly His Asp Phe Glu Gly Leu Ser
85 90 95
tat gtt gct tac aga cca ttc atc gtt gtc gac ttg att aac ttg aga 336
Tyr Val Ala Tyr Arg Pro Phe Ile Val Val Asp Leu Ile Asn Leu Arg
100 105 110
tcc gtt aag gtt gaa gtc gaa aac aat act gct tgg gtt gaa tct ggt 384
Ser Val Lys Val Glu Val Glu Asn Asn Thr Ala Trp Val Glu Ser Gly
115 120 125
gct act ttg ggt gag ttg tat tac aga att ggt gaa aag tct agg acc 432
Ala Thr Leu Gly Glu Leu Tyr Tyr Arg Ile Gly Glu Lys Ser Arg Thr
130 135 140
ttg gct ttt cca gct ggt gtt tgt cca act gtt ggt ata ggt ggt cat 480
Leu Ala Phe Pro Ala Gly Val Cys Pro Thr Val Gly Ile Gly Gly His
145 150 155 160
ttt tct ggt ggt ggt tat ggt ttg atg ttg aga aaa ttt ggt ttg gcc 528
Phe Ser Gly Gly Gly Tyr Gly Leu Met Leu Arg Lys Phe Gly Leu Ala
165 170 175
gct gat aac gtc att gat gct tat ttg gtt gat gcc aac ggt aag gtt 576
Ala Asp Asn Val Ile Asp Ala Tyr Leu Val Asp Ala Asn Gly Lys Val
180 185 190
ttg gac aaa gaa tct atg ggc gaa gat ttg ttc tgg gct att aga ggt 624
Leu Asp Lys Glu Ser Met Gly Glu Asp Leu Phe Trp Ala Ile Arg Gly
195 200 205
ggt ggt ggc ggt tct ttt ggt ata gtt gtt gct tgg aag atc aag ttg 672
Gly Gly Gly Gly Ser Phe Gly Ile Val Val Ala Trp Lys Ile Lys Leu
210 215 220
gtt cca gtt cca gaa act gtt act att tgc tct acc gac aga aac ttg 720
Val Pro Val Pro Glu Thr Val Thr Ile Cys Ser Thr Asp Arg Asn Leu
225 230 235 240
gaa gaa gat gcc att aag ttg atc cac aga tgg caa tac gtt ggt aac 768
Glu Glu Asp Ala Ile Lys Leu Ile His Arg Trp Gln Tyr Val Gly Asn
245 250 255
aaa ttg gac gag aat atc tac ttg ggt atc att ttg act ggt ggt aac 816
Lys Leu Asp Glu Asn Ile Tyr Leu Gly Ile Ile Leu Thr Gly Gly Asn
260 265 270
gct tct act caa gct ggt att act aat cca act gcc aga ttc ttc agc 864
Ala Ser Thr Gln Ala Gly Ile Thr Asn Pro Thr Ala Arg Phe Phe Ser
275 280 285
ttg ttt ttg ggt aga gct gat gaa ttg gtt act acc ttg tct act atc 912
Leu Phe Leu Gly Arg Ala Asp Glu Leu Val Thr Thr Leu Ser Thr Ile
290 295 300
ttc cca gaa ttg ggt ttg atc aag caa gat tgt gtt gaa gct tct tgg 960
Phe Pro Glu Leu Gly Leu Ile Lys Gln Asp Cys Val Glu Ala Ser Trp
305 310 315 320
gtc gaa tcc act ttg att att cca att ggt gtt cag cca atc gaa acc 1008
Val Glu Ser Thr Leu Ile Ile Pro Ile Gly Val Gln Pro Ile Glu Thr
325 330 335
ttg gaa cca tta ttg aac aga act ttg acc acc ttg gac tcc act aag 1056
Leu Glu Pro Leu Leu Asn Arg Thr Leu Thr Thr Leu Asp Ser Thr Lys
340 345 350
att aag tct gat tac atc acc gaa cct att cca gtt gct gct att aag 1104
Ile Lys Ser Asp Tyr Ile Thr Glu Pro Ile Pro Val Ala Ala Ile Lys
355 360 365
gat atc tgg cag aga ttg aaa acc caa gac att gaa acc tct cag ttg 1152
Asp Ile Trp Gln Arg Leu Lys Thr Gln Asp Ile Glu Thr Ser Gln Leu
370 375 380
gtt ttt gtt cca tac ggt ggt aga atg tcc caa atc tct gaa tct gaa 1200
Val Phe Val Pro Tyr Gly Gly Arg Met Ser Gln Ile Ser Glu Ser Glu
385 390 395 400
act cca ttc tct cat aga gcc aga tac ttg ttc aag atc ggt tat gtt 1248
Thr Pro Phe Ser His Arg Ala Arg Tyr Leu Phe Lys Ile Gly Tyr Val
405 410 415
gta ggt tgg aag gac caa tct ttg aag gct aag aag aga cat atc tcc 1296
Val Gly Trp Lys Asp Gln Ser Leu Lys Ala Lys Lys Arg His Ile Ser
420 425 430
tgg ttg aga gaa gtt tat gag tac atg gct cca ttc gtt tct aaa tca 1344
Trp Leu Arg Glu Val Tyr Glu Tyr Met Ala Pro Phe Val Ser Lys Ser
435 440 445
cca aga gct gct tac acc aat tac aga gac ttg gat att ggc tcc aac 1392
Pro Arg Ala Ala Tyr Thr Asn Tyr Arg Asp Leu Asp Ile Gly Ser Asn
450 455 460
aac aaa tac ggt aag acc tct tac aag aga gct tct gtt tgg ggt ttg 1440
Asn Lys Tyr Gly Lys Thr Ser Tyr Lys Arg Ala Ser Val Trp Gly Leu
465 470 475 480
aag tat ttc ggt aac aac ttc gat aga ctg gtt tac gtt aag acc aag 1488
Lys Tyr Phe Gly Asn Asn Phe Asp Arg Leu Val Tyr Val Lys Thr Lys
485 490 495
gtt gat cca tac gat ttc ttc aga cac gaa caa tct att ccc acc ttg 1536
Val Asp Pro Tyr Asp Phe Phe Arg His Glu Gln Ser Ile Pro Thr Leu
500 505 510
<210> 79
<211> 512
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 79
Met Ala Ser Pro Gln Lys Tyr Gln Asn Phe Leu Gln Cys Leu Ser Glu
1 5 10 15
His Ser Ser Lys Ser Tyr Pro Ile Ser Lys Val Val Tyr Thr Pro Ile
20 25 30
Asn Ser Ser Tyr Ser Ser Val Leu Asp Phe Ser Ile Arg Asn Leu Leu
35 40 45
Phe Ser Lys Pro Glu Thr Pro Lys Pro Leu Leu Ile Ile Thr Pro Ser
50 55 60
His Val Ser His Ile Gln Ala Ala Val Ile Cys Ser Lys Ser His Gly
65 70 75 80
Leu Gln Ile Arg Thr Arg Ser Gly Gly His Asp Phe Glu Gly Leu Ser
85 90 95
Tyr Val Ala Tyr Arg Pro Phe Ile Val Val Asp Leu Ile Asn Leu Arg
100 105 110
Ser Val Lys Val Glu Val Glu Asn Asn Thr Ala Trp Val Glu Ser Gly
115 120 125
Ala Thr Leu Gly Glu Leu Tyr Tyr Arg Ile Gly Glu Lys Ser Arg Thr
130 135 140
Leu Ala Phe Pro Ala Gly Val Cys Pro Thr Val Gly Ile Gly Gly His
145 150 155 160
Phe Ser Gly Gly Gly Tyr Gly Leu Met Leu Arg Lys Phe Gly Leu Ala
165 170 175
Ala Asp Asn Val Ile Asp Ala Tyr Leu Val Asp Ala Asn Gly Lys Val
180 185 190
Leu Asp Lys Glu Ser Met Gly Glu Asp Leu Phe Trp Ala Ile Arg Gly
195 200 205
Gly Gly Gly Gly Ser Phe Gly Ile Val Val Ala Trp Lys Ile Lys Leu
210 215 220
Val Pro Val Pro Glu Thr Val Thr Ile Cys Ser Thr Asp Arg Asn Leu
225 230 235 240
Glu Glu Asp Ala Ile Lys Leu Ile His Arg Trp Gln Tyr Val Gly Asn
245 250 255
Lys Leu Asp Glu Asn Ile Tyr Leu Gly Ile Ile Leu Thr Gly Gly Asn
260 265 270
Ala Ser Thr Gln Ala Gly Ile Thr Asn Pro Thr Ala Arg Phe Phe Ser
275 280 285
Leu Phe Leu Gly Arg Ala Asp Glu Leu Val Thr Thr Leu Ser Thr Ile
290 295 300
Phe Pro Glu Leu Gly Leu Ile Lys Gln Asp Cys Val Glu Ala Ser Trp
305 310 315 320
Val Glu Ser Thr Leu Ile Ile Pro Ile Gly Val Gln Pro Ile Glu Thr
325 330 335
Leu Glu Pro Leu Leu Asn Arg Thr Leu Thr Thr Leu Asp Ser Thr Lys
340 345 350
Ile Lys Ser Asp Tyr Ile Thr Glu Pro Ile Pro Val Ala Ala Ile Lys
355 360 365
Asp Ile Trp Gln Arg Leu Lys Thr Gln Asp Ile Glu Thr Ser Gln Leu
370 375 380
Val Phe Val Pro Tyr Gly Gly Arg Met Ser Gln Ile Ser Glu Ser Glu
385 390 395 400
Thr Pro Phe Ser His Arg Ala Arg Tyr Leu Phe Lys Ile Gly Tyr Val
405 410 415
Val Gly Trp Lys Asp Gln Ser Leu Lys Ala Lys Lys Arg His Ile Ser
420 425 430
Trp Leu Arg Glu Val Tyr Glu Tyr Met Ala Pro Phe Val Ser Lys Ser
435 440 445
Pro Arg Ala Ala Tyr Thr Asn Tyr Arg Asp Leu Asp Ile Gly Ser Asn
450 455 460
Asn Lys Tyr Gly Lys Thr Ser Tyr Lys Arg Ala Ser Val Trp Gly Leu
465 470 475 480
Lys Tyr Phe Gly Asn Asn Phe Asp Arg Leu Val Tyr Val Lys Thr Lys
485 490 495
Val Asp Pro Tyr Asp Phe Phe Arg His Glu Gln Ser Ile Pro Thr Leu
500 505 510
<210> 80
<211> 1560
<212> DNA
<213> 人工序列
<220>
<223> CBS99-A0A1S3BDA8-无信号肽-strep标签
<220>
<221> CDS
<222> (1)..(1560)
<400> 80
atg gct tct cca caa aag tac caa aat ttc ttg cag tgc ttg tcc gaa 48
Met Ala Ser Pro Gln Lys Tyr Gln Asn Phe Leu Gln Cys Leu Ser Glu
1 5 10 15
cac tct tct aaa tct tac cca atc tcc aag gtt gtc tat acc cca atc 96
His Ser Ser Lys Ser Tyr Pro Ile Ser Lys Val Val Tyr Thr Pro Ile
20 25 30
aat tcc tct tac tcc tct gtt ttg gac ttc tcc att agg aac ttg ttg 144
Asn Ser Ser Tyr Ser Ser Val Leu Asp Phe Ser Ile Arg Asn Leu Leu
35 40 45
ttc tct aaa cca gaa acg cca aag cct ttg ttg att att act cca tct 192
Phe Ser Lys Pro Glu Thr Pro Lys Pro Leu Leu Ile Ile Thr Pro Ser
50 55 60
cac gtg tcc cat att caa gct gct gtt att tgt tct aag tcc cat ggc 240
His Val Ser His Ile Gln Ala Ala Val Ile Cys Ser Lys Ser His Gly
65 70 75 80
ttg caa atc aga act aga tct ggt ggt cat gat ttc gaa ggt ttg tct 288
Leu Gln Ile Arg Thr Arg Ser Gly Gly His Asp Phe Glu Gly Leu Ser
85 90 95
tat gtt gct tac aga cca ttc atc gtt gtc gac ttg att aac ttg aga 336
Tyr Val Ala Tyr Arg Pro Phe Ile Val Val Asp Leu Ile Asn Leu Arg
100 105 110
tcc gtt aag gtt gaa gtc gaa aac aat act gct tgg gtt gaa tct ggt 384
Ser Val Lys Val Glu Val Glu Asn Asn Thr Ala Trp Val Glu Ser Gly
115 120 125
gct act ttg ggt gag ttg tat tac aga att ggt gaa aag tct agg acc 432
Ala Thr Leu Gly Glu Leu Tyr Tyr Arg Ile Gly Glu Lys Ser Arg Thr
130 135 140
ttg gct ttt cca gct ggt gtt tgt cca act gtt ggt ata ggt ggt cat 480
Leu Ala Phe Pro Ala Gly Val Cys Pro Thr Val Gly Ile Gly Gly His
145 150 155 160
ttt tct ggt ggt ggt tat ggt ttg atg ttg aga aaa ttt ggt ttg gcc 528
Phe Ser Gly Gly Gly Tyr Gly Leu Met Leu Arg Lys Phe Gly Leu Ala
165 170 175
gct gat aac gtc att gat gct tat ttg gtt gat gcc aac ggt aag gtt 576
Ala Asp Asn Val Ile Asp Ala Tyr Leu Val Asp Ala Asn Gly Lys Val
180 185 190
ttg gac aaa gaa tct atg ggc gaa gat ttg ttc tgg gct att aga ggt 624
Leu Asp Lys Glu Ser Met Gly Glu Asp Leu Phe Trp Ala Ile Arg Gly
195 200 205
ggt ggt ggc ggt tct ttt ggt ata gtt gtt gct tgg aag atc aag ttg 672
Gly Gly Gly Gly Ser Phe Gly Ile Val Val Ala Trp Lys Ile Lys Leu
210 215 220
gtt cca gtt cca gaa act gtt act att tgc tct acc gac aga aac ttg 720
Val Pro Val Pro Glu Thr Val Thr Ile Cys Ser Thr Asp Arg Asn Leu
225 230 235 240
gaa gaa gat gcc att aag ttg atc cac aga tgg caa tac gtt ggt aac 768
Glu Glu Asp Ala Ile Lys Leu Ile His Arg Trp Gln Tyr Val Gly Asn
245 250 255
aaa ttg gac gag aat atc tac ttg ggt atc att ttg act ggt ggt aac 816
Lys Leu Asp Glu Asn Ile Tyr Leu Gly Ile Ile Leu Thr Gly Gly Asn
260 265 270
gct tct act caa gct ggt att act aat cca act gcc aga ttc ttc agc 864
Ala Ser Thr Gln Ala Gly Ile Thr Asn Pro Thr Ala Arg Phe Phe Ser
275 280 285
ttg ttt ttg ggt aga gct gat gaa ttg gtt act acc ttg tct act atc 912
Leu Phe Leu Gly Arg Ala Asp Glu Leu Val Thr Thr Leu Ser Thr Ile
290 295 300
ttc cca gaa ttg ggt ttg atc aag caa gat tgt gtt gaa gct tct tgg 960
Phe Pro Glu Leu Gly Leu Ile Lys Gln Asp Cys Val Glu Ala Ser Trp
305 310 315 320
gtc gaa tcc act ttg att att cca att ggt gtt cag cca atc gaa acc 1008
Val Glu Ser Thr Leu Ile Ile Pro Ile Gly Val Gln Pro Ile Glu Thr
325 330 335
ttg gaa cca tta ttg aac aga act ttg acc acc ttg gac tcc act aag 1056
Leu Glu Pro Leu Leu Asn Arg Thr Leu Thr Thr Leu Asp Ser Thr Lys
340 345 350
att aag tct gat tac atc acc gaa cct att cca gtt gct gct att aag 1104
Ile Lys Ser Asp Tyr Ile Thr Glu Pro Ile Pro Val Ala Ala Ile Lys
355 360 365
gat atc tgg cag aga ttg aaa acc caa gac att gaa acc tct cag ttg 1152
Asp Ile Trp Gln Arg Leu Lys Thr Gln Asp Ile Glu Thr Ser Gln Leu
370 375 380
gtt ttt gtt cca tac ggt ggt aga atg tcc caa atc tct gaa tct gaa 1200
Val Phe Val Pro Tyr Gly Gly Arg Met Ser Gln Ile Ser Glu Ser Glu
385 390 395 400
act cca ttc tct cat aga gcc aga tac ttg ttc aag atc ggt tat gtt 1248
Thr Pro Phe Ser His Arg Ala Arg Tyr Leu Phe Lys Ile Gly Tyr Val
405 410 415
gta ggt tgg aag gac caa tct ttg aag gct aag aag aga cat atc tcc 1296
Val Gly Trp Lys Asp Gln Ser Leu Lys Ala Lys Lys Arg His Ile Ser
420 425 430
tgg ttg aga gaa gtt tat gag tac atg gct cca ttc gtt tct aaa tca 1344
Trp Leu Arg Glu Val Tyr Glu Tyr Met Ala Pro Phe Val Ser Lys Ser
435 440 445
cca aga gct gct tac acc aat tac aga gac ttg gat att ggc tcc aac 1392
Pro Arg Ala Ala Tyr Thr Asn Tyr Arg Asp Leu Asp Ile Gly Ser Asn
450 455 460
aac aaa tac ggt aag acc tct tac aag aga gct tct gtt tgg ggt ttg 1440
Asn Lys Tyr Gly Lys Thr Ser Tyr Lys Arg Ala Ser Val Trp Gly Leu
465 470 475 480
aag tat ttc ggt aac aac ttc gat aga ctg gtt tac gtt aag acc aag 1488
Lys Tyr Phe Gly Asn Asn Phe Asp Arg Leu Val Tyr Val Lys Thr Lys
485 490 495
gtt gat cca tac gat ttc ttc aga cac gaa caa tct att ccc acc ttg 1536
Val Asp Pro Tyr Asp Phe Phe Arg His Glu Gln Ser Ile Pro Thr Leu
500 505 510
tgg tca cat cca caa ttt gaa aag 1560
Trp Ser His Pro Gln Phe Glu Lys
515 520
<210> 81
<211> 520
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 81
Met Ala Ser Pro Gln Lys Tyr Gln Asn Phe Leu Gln Cys Leu Ser Glu
1 5 10 15
His Ser Ser Lys Ser Tyr Pro Ile Ser Lys Val Val Tyr Thr Pro Ile
20 25 30
Asn Ser Ser Tyr Ser Ser Val Leu Asp Phe Ser Ile Arg Asn Leu Leu
35 40 45
Phe Ser Lys Pro Glu Thr Pro Lys Pro Leu Leu Ile Ile Thr Pro Ser
50 55 60
His Val Ser His Ile Gln Ala Ala Val Ile Cys Ser Lys Ser His Gly
65 70 75 80
Leu Gln Ile Arg Thr Arg Ser Gly Gly His Asp Phe Glu Gly Leu Ser
85 90 95
Tyr Val Ala Tyr Arg Pro Phe Ile Val Val Asp Leu Ile Asn Leu Arg
100 105 110
Ser Val Lys Val Glu Val Glu Asn Asn Thr Ala Trp Val Glu Ser Gly
115 120 125
Ala Thr Leu Gly Glu Leu Tyr Tyr Arg Ile Gly Glu Lys Ser Arg Thr
130 135 140
Leu Ala Phe Pro Ala Gly Val Cys Pro Thr Val Gly Ile Gly Gly His
145 150 155 160
Phe Ser Gly Gly Gly Tyr Gly Leu Met Leu Arg Lys Phe Gly Leu Ala
165 170 175
Ala Asp Asn Val Ile Asp Ala Tyr Leu Val Asp Ala Asn Gly Lys Val
180 185 190
Leu Asp Lys Glu Ser Met Gly Glu Asp Leu Phe Trp Ala Ile Arg Gly
195 200 205
Gly Gly Gly Gly Ser Phe Gly Ile Val Val Ala Trp Lys Ile Lys Leu
210 215 220
Val Pro Val Pro Glu Thr Val Thr Ile Cys Ser Thr Asp Arg Asn Leu
225 230 235 240
Glu Glu Asp Ala Ile Lys Leu Ile His Arg Trp Gln Tyr Val Gly Asn
245 250 255
Lys Leu Asp Glu Asn Ile Tyr Leu Gly Ile Ile Leu Thr Gly Gly Asn
260 265 270
Ala Ser Thr Gln Ala Gly Ile Thr Asn Pro Thr Ala Arg Phe Phe Ser
275 280 285
Leu Phe Leu Gly Arg Ala Asp Glu Leu Val Thr Thr Leu Ser Thr Ile
290 295 300
Phe Pro Glu Leu Gly Leu Ile Lys Gln Asp Cys Val Glu Ala Ser Trp
305 310 315 320
Val Glu Ser Thr Leu Ile Ile Pro Ile Gly Val Gln Pro Ile Glu Thr
325 330 335
Leu Glu Pro Leu Leu Asn Arg Thr Leu Thr Thr Leu Asp Ser Thr Lys
340 345 350
Ile Lys Ser Asp Tyr Ile Thr Glu Pro Ile Pro Val Ala Ala Ile Lys
355 360 365
Asp Ile Trp Gln Arg Leu Lys Thr Gln Asp Ile Glu Thr Ser Gln Leu
370 375 380
Val Phe Val Pro Tyr Gly Gly Arg Met Ser Gln Ile Ser Glu Ser Glu
385 390 395 400
Thr Pro Phe Ser His Arg Ala Arg Tyr Leu Phe Lys Ile Gly Tyr Val
405 410 415
Val Gly Trp Lys Asp Gln Ser Leu Lys Ala Lys Lys Arg His Ile Ser
420 425 430
Trp Leu Arg Glu Val Tyr Glu Tyr Met Ala Pro Phe Val Ser Lys Ser
435 440 445
Pro Arg Ala Ala Tyr Thr Asn Tyr Arg Asp Leu Asp Ile Gly Ser Asn
450 455 460
Asn Lys Tyr Gly Lys Thr Ser Tyr Lys Arg Ala Ser Val Trp Gly Leu
465 470 475 480
Lys Tyr Phe Gly Asn Asn Phe Asp Arg Leu Val Tyr Val Lys Thr Lys
485 490 495
Val Asp Pro Tyr Asp Phe Phe Arg His Glu Gln Ser Ile Pro Thr Leu
500 505 510
Trp Ser His Pro Gln Phe Glu Lys
515 520
<210> 82
<211> 1617
<212> DNA
<213> 人工序列
<220>
<223> CBS99-A0A1S3BDA8- 截断alpha因子-无信号肽-strep标签
<220>
<221> CDS
<222> (1)..(1617)
<400> 82
atg aga ttt cct tcc atc ttc acg gct gtg cta ttt gca gca tcc tcc 48
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser
1 5 10 15
gca ctt gca atg gct tct cca caa aag tac caa aat ttc ttg cag tgc 96
Ala Leu Ala Met Ala Ser Pro Gln Lys Tyr Gln Asn Phe Leu Gln Cys
20 25 30
ttg tcc gaa cac tct tct aaa tct tac cca atc tcc aag gtt gtc tat 144
Leu Ser Glu His Ser Ser Lys Ser Tyr Pro Ile Ser Lys Val Val Tyr
35 40 45
acc cca atc aat tcc tct tac tcc tct gtt ttg gac ttc tcc att agg 192
Thr Pro Ile Asn Ser Ser Tyr Ser Ser Val Leu Asp Phe Ser Ile Arg
50 55 60
aac ttg ttg ttc tct aaa cca gaa acg cca aag cct ttg ttg att att 240
Asn Leu Leu Phe Ser Lys Pro Glu Thr Pro Lys Pro Leu Leu Ile Ile
65 70 75 80
act cca tct cac gtg tcc cat att caa gct gct gtt att tgt tct aag 288
Thr Pro Ser His Val Ser His Ile Gln Ala Ala Val Ile Cys Ser Lys
85 90 95
tcc cat ggc ttg caa atc aga act aga tct ggt ggt cat gat ttc gaa 336
Ser His Gly Leu Gln Ile Arg Thr Arg Ser Gly Gly His Asp Phe Glu
100 105 110
ggt ttg tct tat gtt gct tac aga cca ttc atc gtt gtc gac ttg att 384
Gly Leu Ser Tyr Val Ala Tyr Arg Pro Phe Ile Val Val Asp Leu Ile
115 120 125
aac ttg aga tcc gtt aag gtt gaa gtc gaa aac aat act gct tgg gtt 432
Asn Leu Arg Ser Val Lys Val Glu Val Glu Asn Asn Thr Ala Trp Val
130 135 140
gaa tct ggt gct act ttg ggt gag ttg tat tac aga att ggt gaa aag 480
Glu Ser Gly Ala Thr Leu Gly Glu Leu Tyr Tyr Arg Ile Gly Glu Lys
145 150 155 160
tct agg acc ttg gct ttt cca gct ggt gtt tgt cca act gtt ggt ata 528
Ser Arg Thr Leu Ala Phe Pro Ala Gly Val Cys Pro Thr Val Gly Ile
165 170 175
ggt ggt cat ttt tct ggt ggt ggt tat ggt ttg atg ttg aga aaa ttt 576
Gly Gly His Phe Ser Gly Gly Gly Tyr Gly Leu Met Leu Arg Lys Phe
180 185 190
ggt ttg gcc gct gat aac gtc att gat gct tat ttg gtt gat gcc aac 624
Gly Leu Ala Ala Asp Asn Val Ile Asp Ala Tyr Leu Val Asp Ala Asn
195 200 205
ggt aag gtt ttg gac aaa gaa tct atg ggc gaa gat ttg ttc tgg gct 672
Gly Lys Val Leu Asp Lys Glu Ser Met Gly Glu Asp Leu Phe Trp Ala
210 215 220
att aga ggt ggt ggt ggc ggt tct ttt ggt ata gtt gtt gct tgg aag 720
Ile Arg Gly Gly Gly Gly Gly Ser Phe Gly Ile Val Val Ala Trp Lys
225 230 235 240
atc aag ttg gtt cca gtt cca gaa act gtt act att tgc tct acc gac 768
Ile Lys Leu Val Pro Val Pro Glu Thr Val Thr Ile Cys Ser Thr Asp
245 250 255
aga aac ttg gaa gaa gat gcc att aag ttg atc cac aga tgg caa tac 816
Arg Asn Leu Glu Glu Asp Ala Ile Lys Leu Ile His Arg Trp Gln Tyr
260 265 270
gtt ggt aac aaa ttg gac gag aat atc tac ttg ggt atc att ttg act 864
Val Gly Asn Lys Leu Asp Glu Asn Ile Tyr Leu Gly Ile Ile Leu Thr
275 280 285
ggt ggt aac gct tct act caa gct ggt att act aat cca act gcc aga 912
Gly Gly Asn Ala Ser Thr Gln Ala Gly Ile Thr Asn Pro Thr Ala Arg
290 295 300
ttc ttc agc ttg ttt ttg ggt aga gct gat gaa ttg gtt act acc ttg 960
Phe Phe Ser Leu Phe Leu Gly Arg Ala Asp Glu Leu Val Thr Thr Leu
305 310 315 320
tct act atc ttc cca gaa ttg ggt ttg atc aag caa gat tgt gtt gaa 1008
Ser Thr Ile Phe Pro Glu Leu Gly Leu Ile Lys Gln Asp Cys Val Glu
325 330 335
gct tct tgg gtc gaa tcc act ttg att att cca att ggt gtt cag cca 1056
Ala Ser Trp Val Glu Ser Thr Leu Ile Ile Pro Ile Gly Val Gln Pro
340 345 350
atc gaa acc ttg gaa cca tta ttg aac aga act ttg acc acc ttg gac 1104
Ile Glu Thr Leu Glu Pro Leu Leu Asn Arg Thr Leu Thr Thr Leu Asp
355 360 365
tcc act aag att aag tct gat tac atc acc gaa cct att cca gtt gct 1152
Ser Thr Lys Ile Lys Ser Asp Tyr Ile Thr Glu Pro Ile Pro Val Ala
370 375 380
gct att aag gat atc tgg cag aga ttg aaa acc caa gac att gaa acc 1200
Ala Ile Lys Asp Ile Trp Gln Arg Leu Lys Thr Gln Asp Ile Glu Thr
385 390 395 400
tct cag ttg gtt ttt gtt cca tac ggt ggt aga atg tcc caa atc tct 1248
Ser Gln Leu Val Phe Val Pro Tyr Gly Gly Arg Met Ser Gln Ile Ser
405 410 415
gaa tct gaa act cca ttc tct cat aga gcc aga tac ttg ttc aag atc 1296
Glu Ser Glu Thr Pro Phe Ser His Arg Ala Arg Tyr Leu Phe Lys Ile
420 425 430
ggt tat gtt gta ggt tgg aag gac caa tct ttg aag gct aag aag aga 1344
Gly Tyr Val Val Gly Trp Lys Asp Gln Ser Leu Lys Ala Lys Lys Arg
435 440 445
cat atc tcc tgg ttg aga gaa gtt tat gag tac atg gct cca ttc gtt 1392
His Ile Ser Trp Leu Arg Glu Val Tyr Glu Tyr Met Ala Pro Phe Val
450 455 460
tct aaa tca cca aga gct gct tac acc aat tac aga gac ttg gat att 1440
Ser Lys Ser Pro Arg Ala Ala Tyr Thr Asn Tyr Arg Asp Leu Asp Ile
465 470 475 480
ggc tcc aac aac aaa tac ggt aag acc tct tac aag aga gct tct gtt 1488
Gly Ser Asn Asn Lys Tyr Gly Lys Thr Ser Tyr Lys Arg Ala Ser Val
485 490 495
tgg ggt ttg aag tat ttc ggt aac aac ttc gat aga ctg gtt tac gtt 1536
Trp Gly Leu Lys Tyr Phe Gly Asn Asn Phe Asp Arg Leu Val Tyr Val
500 505 510
aag acc aag gtt gat cca tac gat ttc ttc aga cac gaa caa tct att 1584
Lys Thr Lys Val Asp Pro Tyr Asp Phe Phe Arg His Glu Gln Ser Ile
515 520 525
ccc acc ttg tgg tca cat cca caa ttt gaa aag 1617
Pro Thr Leu Trp Ser His Pro Gln Phe Glu Lys
530 535
<210> 83
<211> 539
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 83
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser
1 5 10 15
Ala Leu Ala Met Ala Ser Pro Gln Lys Tyr Gln Asn Phe Leu Gln Cys
20 25 30
Leu Ser Glu His Ser Ser Lys Ser Tyr Pro Ile Ser Lys Val Val Tyr
35 40 45
Thr Pro Ile Asn Ser Ser Tyr Ser Ser Val Leu Asp Phe Ser Ile Arg
50 55 60
Asn Leu Leu Phe Ser Lys Pro Glu Thr Pro Lys Pro Leu Leu Ile Ile
65 70 75 80
Thr Pro Ser His Val Ser His Ile Gln Ala Ala Val Ile Cys Ser Lys
85 90 95
Ser His Gly Leu Gln Ile Arg Thr Arg Ser Gly Gly His Asp Phe Glu
100 105 110
Gly Leu Ser Tyr Val Ala Tyr Arg Pro Phe Ile Val Val Asp Leu Ile
115 120 125
Asn Leu Arg Ser Val Lys Val Glu Val Glu Asn Asn Thr Ala Trp Val
130 135 140
Glu Ser Gly Ala Thr Leu Gly Glu Leu Tyr Tyr Arg Ile Gly Glu Lys
145 150 155 160
Ser Arg Thr Leu Ala Phe Pro Ala Gly Val Cys Pro Thr Val Gly Ile
165 170 175
Gly Gly His Phe Ser Gly Gly Gly Tyr Gly Leu Met Leu Arg Lys Phe
180 185 190
Gly Leu Ala Ala Asp Asn Val Ile Asp Ala Tyr Leu Val Asp Ala Asn
195 200 205
Gly Lys Val Leu Asp Lys Glu Ser Met Gly Glu Asp Leu Phe Trp Ala
210 215 220
Ile Arg Gly Gly Gly Gly Gly Ser Phe Gly Ile Val Val Ala Trp Lys
225 230 235 240
Ile Lys Leu Val Pro Val Pro Glu Thr Val Thr Ile Cys Ser Thr Asp
245 250 255
Arg Asn Leu Glu Glu Asp Ala Ile Lys Leu Ile His Arg Trp Gln Tyr
260 265 270
Val Gly Asn Lys Leu Asp Glu Asn Ile Tyr Leu Gly Ile Ile Leu Thr
275 280 285
Gly Gly Asn Ala Ser Thr Gln Ala Gly Ile Thr Asn Pro Thr Ala Arg
290 295 300
Phe Phe Ser Leu Phe Leu Gly Arg Ala Asp Glu Leu Val Thr Thr Leu
305 310 315 320
Ser Thr Ile Phe Pro Glu Leu Gly Leu Ile Lys Gln Asp Cys Val Glu
325 330 335
Ala Ser Trp Val Glu Ser Thr Leu Ile Ile Pro Ile Gly Val Gln Pro
340 345 350
Ile Glu Thr Leu Glu Pro Leu Leu Asn Arg Thr Leu Thr Thr Leu Asp
355 360 365
Ser Thr Lys Ile Lys Ser Asp Tyr Ile Thr Glu Pro Ile Pro Val Ala
370 375 380
Ala Ile Lys Asp Ile Trp Gln Arg Leu Lys Thr Gln Asp Ile Glu Thr
385 390 395 400
Ser Gln Leu Val Phe Val Pro Tyr Gly Gly Arg Met Ser Gln Ile Ser
405 410 415
Glu Ser Glu Thr Pro Phe Ser His Arg Ala Arg Tyr Leu Phe Lys Ile
420 425 430
Gly Tyr Val Val Gly Trp Lys Asp Gln Ser Leu Lys Ala Lys Lys Arg
435 440 445
His Ile Ser Trp Leu Arg Glu Val Tyr Glu Tyr Met Ala Pro Phe Val
450 455 460
Ser Lys Ser Pro Arg Ala Ala Tyr Thr Asn Tyr Arg Asp Leu Asp Ile
465 470 475 480
Gly Ser Asn Asn Lys Tyr Gly Lys Thr Ser Tyr Lys Arg Ala Ser Val
485 490 495
Trp Gly Leu Lys Tyr Phe Gly Asn Asn Phe Asp Arg Leu Val Tyr Val
500 505 510
Lys Thr Lys Val Asp Pro Tyr Asp Phe Phe Arg His Glu Gln Ser Ile
515 520 525
Pro Thr Leu Trp Ser His Pro Gln Phe Glu Lys
530 535
<210> 84
<211> 1827
<212> DNA
<213> 人工序列
<220>
<223> CBS99-A0A1S3BDA8-全长alpha因子-无信号肽-strep标签
<220>
<221> CDS
<222> (1)..(1827)
<400> 84
atg aga ttt cct tca att ttt act gct gtt tta ttc gca gca tcc tcc 48
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser
1 5 10 15
gca tta gct gct cca gtc aac act aca aca gaa gat gaa acg gca caa 96
Ala Leu Ala Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln
20 25 30
att ccg gct gaa gct gtc atc ggt tac tca gat tta gaa ggg gat ttc 144
Ile Pro Ala Glu Ala Val Ile Gly Tyr Ser Asp Leu Glu Gly Asp Phe
35 40 45
gat gtt gct gtt ttg cca ttt tcc aac agc aca aat aac ggg tta ttg 192
Asp Val Ala Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu
50 55 60
ttt ata aat act act att gcc agc att gct gct aaa gaa gaa ggg gta 240
Phe Ile Asn Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val
65 70 75 80
tct ctc gag aaa aga gag gct gaa gct atg gct tct cca caa aag tac 288
Ser Leu Glu Lys Arg Glu Ala Glu Ala Met Ala Ser Pro Gln Lys Tyr
85 90 95
caa aat ttc ttg cag tgc ttg tcc gaa cac tct tct aaa tct tac cca 336
Gln Asn Phe Leu Gln Cys Leu Ser Glu His Ser Ser Lys Ser Tyr Pro
100 105 110
atc tcc aag gtt gtc tat acc cca atc aat tcc tct tac tcc tct gtt 384
Ile Ser Lys Val Val Tyr Thr Pro Ile Asn Ser Ser Tyr Ser Ser Val
115 120 125
ttg gac ttc tcc att agg aac ttg ttg ttc tct aaa cca gaa acg cca 432
Leu Asp Phe Ser Ile Arg Asn Leu Leu Phe Ser Lys Pro Glu Thr Pro
130 135 140
aag cct ttg ttg att att act cca tct cac gtg tcc cat att caa gct 480
Lys Pro Leu Leu Ile Ile Thr Pro Ser His Val Ser His Ile Gln Ala
145 150 155 160
gct gtt att tgt tct aag tcc cat ggc ttg caa atc aga act aga tct 528
Ala Val Ile Cys Ser Lys Ser His Gly Leu Gln Ile Arg Thr Arg Ser
165 170 175
ggt ggt cat gat ttc gaa ggt ttg tct tat gtt gct tac aga cca ttc 576
Gly Gly His Asp Phe Glu Gly Leu Ser Tyr Val Ala Tyr Arg Pro Phe
180 185 190
atc gtt gtc gac ttg att aac ttg aga tcc gtt aag gtt gaa gtc gaa 624
Ile Val Val Asp Leu Ile Asn Leu Arg Ser Val Lys Val Glu Val Glu
195 200 205
aac aat act gct tgg gtt gaa tct ggt gct act ttg ggt gag ttg tat 672
Asn Asn Thr Ala Trp Val Glu Ser Gly Ala Thr Leu Gly Glu Leu Tyr
210 215 220
tac aga att ggt gaa aag tct agg acc ttg gct ttt cca gct ggt gtt 720
Tyr Arg Ile Gly Glu Lys Ser Arg Thr Leu Ala Phe Pro Ala Gly Val
225 230 235 240
tgt cca act gtt ggt ata ggt ggt cat ttt tct ggt ggt ggt tat ggt 768
Cys Pro Thr Val Gly Ile Gly Gly His Phe Ser Gly Gly Gly Tyr Gly
245 250 255
ttg atg ttg aga aaa ttt ggt ttg gcc gct gat aac gtc att gat gct 816
Leu Met Leu Arg Lys Phe Gly Leu Ala Ala Asp Asn Val Ile Asp Ala
260 265 270
tat ttg gtt gat gcc aac ggt aag gtt ttg gac aaa gaa tct atg ggc 864
Tyr Leu Val Asp Ala Asn Gly Lys Val Leu Asp Lys Glu Ser Met Gly
275 280 285
gaa gat ttg ttc tgg gct att aga ggt ggt ggt ggc ggt tct ttt ggt 912
Glu Asp Leu Phe Trp Ala Ile Arg Gly Gly Gly Gly Gly Ser Phe Gly
290 295 300
ata gtt gtt gct tgg aag atc aag ttg gtt cca gtt cca gaa act gtt 960
Ile Val Val Ala Trp Lys Ile Lys Leu Val Pro Val Pro Glu Thr Val
305 310 315 320
act att tgc tct acc gac aga aac ttg gaa gaa gat gcc att aag ttg 1008
Thr Ile Cys Ser Thr Asp Arg Asn Leu Glu Glu Asp Ala Ile Lys Leu
325 330 335
atc cac aga tgg caa tac gtt ggt aac aaa ttg gac gag aat atc tac 1056
Ile His Arg Trp Gln Tyr Val Gly Asn Lys Leu Asp Glu Asn Ile Tyr
340 345 350
ttg ggt atc att ttg act ggt ggt aac gct tct act caa gct ggt att 1104
Leu Gly Ile Ile Leu Thr Gly Gly Asn Ala Ser Thr Gln Ala Gly Ile
355 360 365
act aat cca act gcc aga ttc ttc agc ttg ttt ttg ggt aga gct gat 1152
Thr Asn Pro Thr Ala Arg Phe Phe Ser Leu Phe Leu Gly Arg Ala Asp
370 375 380
gaa ttg gtt act acc ttg tct act atc ttc cca gaa ttg ggt ttg atc 1200
Glu Leu Val Thr Thr Leu Ser Thr Ile Phe Pro Glu Leu Gly Leu Ile
385 390 395 400
aag caa gat tgt gtt gaa gct tct tgg gtc gaa tcc act ttg att att 1248
Lys Gln Asp Cys Val Glu Ala Ser Trp Val Glu Ser Thr Leu Ile Ile
405 410 415
cca att ggt gtt cag cca atc gaa acc ttg gaa cca tta ttg aac aga 1296
Pro Ile Gly Val Gln Pro Ile Glu Thr Leu Glu Pro Leu Leu Asn Arg
420 425 430
act ttg acc acc ttg gac tcc act aag att aag tct gat tac atc acc 1344
Thr Leu Thr Thr Leu Asp Ser Thr Lys Ile Lys Ser Asp Tyr Ile Thr
435 440 445
gaa cct att cca gtt gct gct att aag gat atc tgg cag aga ttg aaa 1392
Glu Pro Ile Pro Val Ala Ala Ile Lys Asp Ile Trp Gln Arg Leu Lys
450 455 460
acc caa gac att gaa acc tct cag ttg gtt ttt gtt cca tac ggt ggt 1440
Thr Gln Asp Ile Glu Thr Ser Gln Leu Val Phe Val Pro Tyr Gly Gly
465 470 475 480
aga atg tcc caa atc tct gaa tct gaa act cca ttc tct cat aga gcc 1488
Arg Met Ser Gln Ile Ser Glu Ser Glu Thr Pro Phe Ser His Arg Ala
485 490 495
aga tac ttg ttc aag atc ggt tat gtt gta ggt tgg aag gac caa tct 1536
Arg Tyr Leu Phe Lys Ile Gly Tyr Val Val Gly Trp Lys Asp Gln Ser
500 505 510
ttg aag gct aag aag aga cat atc tcc tgg ttg aga gaa gtt tat gag 1584
Leu Lys Ala Lys Lys Arg His Ile Ser Trp Leu Arg Glu Val Tyr Glu
515 520 525
tac atg gct cca ttc gtt tct aaa tca cca aga gct gct tac acc aat 1632
Tyr Met Ala Pro Phe Val Ser Lys Ser Pro Arg Ala Ala Tyr Thr Asn
530 535 540
tac aga gac ttg gat att ggc tcc aac aac aaa tac ggt aag acc tct 1680
Tyr Arg Asp Leu Asp Ile Gly Ser Asn Asn Lys Tyr Gly Lys Thr Ser
545 550 555 560
tac aag aga gct tct gtt tgg ggt ttg aag tat ttc ggt aac aac ttc 1728
Tyr Lys Arg Ala Ser Val Trp Gly Leu Lys Tyr Phe Gly Asn Asn Phe
565 570 575
gat aga ctg gtt tac gtt aag acc aag gtt gat cca tac gat ttc ttc 1776
Asp Arg Leu Val Tyr Val Lys Thr Lys Val Asp Pro Tyr Asp Phe Phe
580 585 590
aga cac gaa caa tct att ccc acc ttg tgg tca cat cca caa ttt gaa 1824
Arg His Glu Gln Ser Ile Pro Thr Leu Trp Ser His Pro Gln Phe Glu
595 600 605
aag 1827
Lys
<210> 85
<211> 609
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 85
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser
1 5 10 15
Ala Leu Ala Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln
20 25 30
Ile Pro Ala Glu Ala Val Ile Gly Tyr Ser Asp Leu Glu Gly Asp Phe
35 40 45
Asp Val Ala Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu
50 55 60
Phe Ile Asn Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val
65 70 75 80
Ser Leu Glu Lys Arg Glu Ala Glu Ala Met Ala Ser Pro Gln Lys Tyr
85 90 95
Gln Asn Phe Leu Gln Cys Leu Ser Glu His Ser Ser Lys Ser Tyr Pro
100 105 110
Ile Ser Lys Val Val Tyr Thr Pro Ile Asn Ser Ser Tyr Ser Ser Val
115 120 125
Leu Asp Phe Ser Ile Arg Asn Leu Leu Phe Ser Lys Pro Glu Thr Pro
130 135 140
Lys Pro Leu Leu Ile Ile Thr Pro Ser His Val Ser His Ile Gln Ala
145 150 155 160
Ala Val Ile Cys Ser Lys Ser His Gly Leu Gln Ile Arg Thr Arg Ser
165 170 175
Gly Gly His Asp Phe Glu Gly Leu Ser Tyr Val Ala Tyr Arg Pro Phe
180 185 190
Ile Val Val Asp Leu Ile Asn Leu Arg Ser Val Lys Val Glu Val Glu
195 200 205
Asn Asn Thr Ala Trp Val Glu Ser Gly Ala Thr Leu Gly Glu Leu Tyr
210 215 220
Tyr Arg Ile Gly Glu Lys Ser Arg Thr Leu Ala Phe Pro Ala Gly Val
225 230 235 240
Cys Pro Thr Val Gly Ile Gly Gly His Phe Ser Gly Gly Gly Tyr Gly
245 250 255
Leu Met Leu Arg Lys Phe Gly Leu Ala Ala Asp Asn Val Ile Asp Ala
260 265 270
Tyr Leu Val Asp Ala Asn Gly Lys Val Leu Asp Lys Glu Ser Met Gly
275 280 285
Glu Asp Leu Phe Trp Ala Ile Arg Gly Gly Gly Gly Gly Ser Phe Gly
290 295 300
Ile Val Val Ala Trp Lys Ile Lys Leu Val Pro Val Pro Glu Thr Val
305 310 315 320
Thr Ile Cys Ser Thr Asp Arg Asn Leu Glu Glu Asp Ala Ile Lys Leu
325 330 335
Ile His Arg Trp Gln Tyr Val Gly Asn Lys Leu Asp Glu Asn Ile Tyr
340 345 350
Leu Gly Ile Ile Leu Thr Gly Gly Asn Ala Ser Thr Gln Ala Gly Ile
355 360 365
Thr Asn Pro Thr Ala Arg Phe Phe Ser Leu Phe Leu Gly Arg Ala Asp
370 375 380
Glu Leu Val Thr Thr Leu Ser Thr Ile Phe Pro Glu Leu Gly Leu Ile
385 390 395 400
Lys Gln Asp Cys Val Glu Ala Ser Trp Val Glu Ser Thr Leu Ile Ile
405 410 415
Pro Ile Gly Val Gln Pro Ile Glu Thr Leu Glu Pro Leu Leu Asn Arg
420 425 430
Thr Leu Thr Thr Leu Asp Ser Thr Lys Ile Lys Ser Asp Tyr Ile Thr
435 440 445
Glu Pro Ile Pro Val Ala Ala Ile Lys Asp Ile Trp Gln Arg Leu Lys
450 455 460
Thr Gln Asp Ile Glu Thr Ser Gln Leu Val Phe Val Pro Tyr Gly Gly
465 470 475 480
Arg Met Ser Gln Ile Ser Glu Ser Glu Thr Pro Phe Ser His Arg Ala
485 490 495
Arg Tyr Leu Phe Lys Ile Gly Tyr Val Val Gly Trp Lys Asp Gln Ser
500 505 510
Leu Lys Ala Lys Lys Arg His Ile Ser Trp Leu Arg Glu Val Tyr Glu
515 520 525
Tyr Met Ala Pro Phe Val Ser Lys Ser Pro Arg Ala Ala Tyr Thr Asn
530 535 540
Tyr Arg Asp Leu Asp Ile Gly Ser Asn Asn Lys Tyr Gly Lys Thr Ser
545 550 555 560
Tyr Lys Arg Ala Ser Val Trp Gly Leu Lys Tyr Phe Gly Asn Asn Phe
565 570 575
Asp Arg Leu Val Tyr Val Lys Thr Lys Val Asp Pro Tyr Asp Phe Phe
580 585 590
Arg His Glu Gln Ser Ile Pro Thr Leu Trp Ser His Pro Gln Phe Glu
595 600 605
Lys
<210> 86
<211> 1608
<212> DNA
<213> 人工序列
<220>
<223> CBS124-A0A1U8FGM5
<220>
<221> CDS
<222> (1)..(1608)
<400> 86
atg ggt aac ttg caa atc atc tgc gtc ttg ttg ttc tct ttg ttc ttg 48
Met Gly Asn Leu Gln Ile Ile Cys Val Leu Leu Phe Ser Leu Phe Leu
1 5 10 15
gct aag tgc tac tcc aga gaa gat ttc ttg aga tgc ttg ttc aag tcc 96
Ala Lys Cys Tyr Ser Arg Glu Asp Phe Leu Arg Cys Leu Phe Lys Ser
20 25 30
tcc gaa tct aac gtt act caa aac atc tac acc cca aac tca ttg acc 144
Ser Glu Ser Asn Val Thr Gln Asn Ile Tyr Thr Pro Asn Ser Leu Thr
35 40 45
tac tcc tct att ttg aag tac gct caa aaa cac tcc aga tgg gtt aat 192
Tyr Ser Ser Ile Leu Lys Tyr Ala Gln Lys His Ser Arg Trp Val Asn
50 55 60
tct tct cag cca aat ttc att gct tct ccc aca aaa gaa tcc gaa atc 240
Ser Ser Gln Pro Asn Phe Ile Ala Ser Pro Thr Lys Glu Ser Glu Ile
65 70 75 80
aag cca gtt att ttg tgc gct aag aag ttg ggt ctg caa atc aaa atc 288
Lys Pro Val Ile Leu Cys Ala Lys Lys Leu Gly Leu Gln Ile Lys Ile
85 90 95
aaa tcc ggt ggt cat gat tac gag ggt att tct tac aga tct gaa ttc 336
Lys Ser Gly Gly His Asp Tyr Glu Gly Ile Ser Tyr Arg Ser Glu Phe
100 105 110
cca ttc gtc atg ttg gac ttg tcc aat ttg aac aag acc gaa atc gac 384
Pro Phe Val Met Leu Asp Leu Ser Asn Leu Asn Lys Thr Glu Ile Asp
115 120 125
ttg aac gaa gaa acc att tgg gtt caa gct ggt gct act ttg ggt caa 432
Leu Asn Glu Glu Thr Ile Trp Val Gln Ala Gly Ala Thr Leu Gly Gln
130 135 140
cta tat tac gct att gcc aac aga tcc aac gtt cat ggt ttt cca ggt 480
Leu Tyr Tyr Ala Ile Ala Asn Arg Ser Asn Val His Gly Phe Pro Gly
145 150 155 160
ggt att tgt ttc tct gtt ggt act ggt ggt ttg att tct ggt ggt ggt 528
Gly Ile Cys Phe Ser Val Gly Thr Gly Gly Leu Ile Ser Gly Gly Gly
165 170 175
tta ggt act atg atg aga aaa ttt ggt ttg gcc gct gat aac gtt gtt 576
Leu Gly Thr Met Met Arg Lys Phe Gly Leu Ala Ala Asp Asn Val Val
180 185 190
gat gct aga gtt atg gat gtc aac ggt aac atc ttg gac cgt aaa aag 624
Asp Ala Arg Val Met Asp Val Asn Gly Asn Ile Leu Asp Arg Lys Lys
195 200 205
atg aag aac gat ttg ttc tgg gca att aga ggt ggt ggt ggc gct tct 672
Met Lys Asn Asp Leu Phe Trp Ala Ile Arg Gly Gly Gly Gly Ala Ser
210 215 220
ttt ggt gtt att ttg gct tgg aaa ttg aag ttg gtt agg gtt cca gaa 720
Phe Gly Val Ile Leu Ala Trp Lys Leu Lys Leu Val Arg Val Pro Glu
225 230 235 240
aag gtt act gtt ttc acc gtc tgc aaa aag ttc gat gat aac cag aac 768
Lys Val Thr Val Phe Thr Val Cys Lys Lys Phe Asp Asp Asn Gln Asn
245 250 255
ttg atc ctg aag tgg gaa aac att gct cat caa ttg cca gat aag ttg 816
Leu Ile Leu Lys Trp Glu Asn Ile Ala His Gln Leu Pro Asp Lys Leu
260 265 270
gtc atc aga gtt gtt atc caa aac gat ggt aca ggt aac gat aag tac 864
Val Ile Arg Val Val Ile Gln Asn Asp Gly Thr Gly Asn Asp Lys Tyr
275 280 285
gtc gaa atg aga ttt caa gcc ttg tat ttg ggt cca gtt gat gaa ttg 912
Val Glu Met Arg Phe Gln Ala Leu Tyr Leu Gly Pro Val Asp Glu Leu
290 295 300
atc ccc ttg ttg aaa gaa aag ttc cca gaa ttc gac ttg gaa aag aag 960
Ile Pro Leu Leu Lys Glu Lys Phe Pro Glu Phe Asp Leu Glu Lys Lys
305 310 315 320
gat tgc ttg caa gaa cca gtt ggt gat aga tct aac aga tcc tgc att 1008
Asp Cys Leu Gln Glu Pro Val Gly Asp Arg Ser Asn Arg Ser Cys Ile
325 330 335
aac aaa gaa tgc aga gaa acc tct tgg atc ggt tct gtc ttg tac ttt 1056
Asn Lys Glu Cys Arg Glu Thr Ser Trp Ile Gly Ser Val Leu Tyr Phe
340 345 350
tac gaa aga gaa acc acc tcc tca ttg gag gtc tta ttg aaa gaa tcc 1104
Tyr Glu Arg Glu Thr Thr Ser Ser Leu Glu Val Leu Leu Lys Glu Ser
355 360 365
att cca aag cag aag tcc tac tac aaa ggt act tct gat ttc gtc aag 1152
Ile Pro Lys Gln Lys Ser Tyr Tyr Lys Gly Thr Ser Asp Phe Val Lys
370 375 380
acc cag att tct gaa tct ggt tgg gaa atg gtt gaa agg ttg ttg ttg 1200
Thr Gln Ile Ser Glu Ser Gly Trp Glu Met Val Glu Arg Leu Leu Leu
385 390 395 400
gaa gaa gaa tcc cca aga att atc ttg gaa cca tta ggt ggt aag ttg 1248
Glu Glu Glu Ser Pro Arg Ile Ile Leu Glu Pro Leu Gly Gly Lys Leu
405 410 415
gac gaa atc tcc gaa tca gaa att cca ttt cca cac aga aaa ggc aac 1296
Asp Glu Ile Ser Glu Ser Glu Ile Pro Phe Pro His Arg Lys Gly Asn
420 425 430
ttg tac aac atc caa tac ttg gtt tac tgg cac gac aac aac gaa aac 1344
Leu Tyr Asn Ile Gln Tyr Leu Val Tyr Trp His Asp Asn Asn Glu Asn
435 440 445
gtt tcc tct caa aaa att gcc tgg atg cgt aag ctg tac aaa gaa atg 1392
Val Ser Ser Gln Lys Ile Ala Trp Met Arg Lys Leu Tyr Lys Glu Met
450 455 460
gaa cca tac gtt gct aac tct cca aga act gct tac ttg aac tac aga 1440
Glu Pro Tyr Val Ala Asn Ser Pro Arg Thr Ala Tyr Leu Asn Tyr Arg
465 470 475 480
gat ttg gac ttc ggt tct aac gat gac aac tac tct tac tcc aaa gct 1488
Asp Leu Asp Phe Gly Ser Asn Asp Asp Asn Tyr Ser Tyr Ser Lys Ala
485 490 495
aaa atc tgg ggc gaa aag tac ttc aac tac aac ttt gaa aga ctg gcc 1536
Lys Ile Trp Gly Glu Lys Tyr Phe Asn Tyr Asn Phe Glu Arg Leu Ala
500 505 510
aag gtt aag tct aag gtt gat cca aac aac ttc ttc aga acc gaa caa 1584
Lys Val Lys Ser Lys Val Asp Pro Asn Asn Phe Phe Arg Thr Glu Gln
515 520 525
tct att ccc ttg tac caa acc tac 1608
Ser Ile Pro Leu Tyr Gln Thr Tyr
530 535
<210> 87
<211> 536
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 87
Met Gly Asn Leu Gln Ile Ile Cys Val Leu Leu Phe Ser Leu Phe Leu
1 5 10 15
Ala Lys Cys Tyr Ser Arg Glu Asp Phe Leu Arg Cys Leu Phe Lys Ser
20 25 30
Ser Glu Ser Asn Val Thr Gln Asn Ile Tyr Thr Pro Asn Ser Leu Thr
35 40 45
Tyr Ser Ser Ile Leu Lys Tyr Ala Gln Lys His Ser Arg Trp Val Asn
50 55 60
Ser Ser Gln Pro Asn Phe Ile Ala Ser Pro Thr Lys Glu Ser Glu Ile
65 70 75 80
Lys Pro Val Ile Leu Cys Ala Lys Lys Leu Gly Leu Gln Ile Lys Ile
85 90 95
Lys Ser Gly Gly His Asp Tyr Glu Gly Ile Ser Tyr Arg Ser Glu Phe
100 105 110
Pro Phe Val Met Leu Asp Leu Ser Asn Leu Asn Lys Thr Glu Ile Asp
115 120 125
Leu Asn Glu Glu Thr Ile Trp Val Gln Ala Gly Ala Thr Leu Gly Gln
130 135 140
Leu Tyr Tyr Ala Ile Ala Asn Arg Ser Asn Val His Gly Phe Pro Gly
145 150 155 160
Gly Ile Cys Phe Ser Val Gly Thr Gly Gly Leu Ile Ser Gly Gly Gly
165 170 175
Leu Gly Thr Met Met Arg Lys Phe Gly Leu Ala Ala Asp Asn Val Val
180 185 190
Asp Ala Arg Val Met Asp Val Asn Gly Asn Ile Leu Asp Arg Lys Lys
195 200 205
Met Lys Asn Asp Leu Phe Trp Ala Ile Arg Gly Gly Gly Gly Ala Ser
210 215 220
Phe Gly Val Ile Leu Ala Trp Lys Leu Lys Leu Val Arg Val Pro Glu
225 230 235 240
Lys Val Thr Val Phe Thr Val Cys Lys Lys Phe Asp Asp Asn Gln Asn
245 250 255
Leu Ile Leu Lys Trp Glu Asn Ile Ala His Gln Leu Pro Asp Lys Leu
260 265 270
Val Ile Arg Val Val Ile Gln Asn Asp Gly Thr Gly Asn Asp Lys Tyr
275 280 285
Val Glu Met Arg Phe Gln Ala Leu Tyr Leu Gly Pro Val Asp Glu Leu
290 295 300
Ile Pro Leu Leu Lys Glu Lys Phe Pro Glu Phe Asp Leu Glu Lys Lys
305 310 315 320
Asp Cys Leu Gln Glu Pro Val Gly Asp Arg Ser Asn Arg Ser Cys Ile
325 330 335
Asn Lys Glu Cys Arg Glu Thr Ser Trp Ile Gly Ser Val Leu Tyr Phe
340 345 350
Tyr Glu Arg Glu Thr Thr Ser Ser Leu Glu Val Leu Leu Lys Glu Ser
355 360 365
Ile Pro Lys Gln Lys Ser Tyr Tyr Lys Gly Thr Ser Asp Phe Val Lys
370 375 380
Thr Gln Ile Ser Glu Ser Gly Trp Glu Met Val Glu Arg Leu Leu Leu
385 390 395 400
Glu Glu Glu Ser Pro Arg Ile Ile Leu Glu Pro Leu Gly Gly Lys Leu
405 410 415
Asp Glu Ile Ser Glu Ser Glu Ile Pro Phe Pro His Arg Lys Gly Asn
420 425 430
Leu Tyr Asn Ile Gln Tyr Leu Val Tyr Trp His Asp Asn Asn Glu Asn
435 440 445
Val Ser Ser Gln Lys Ile Ala Trp Met Arg Lys Leu Tyr Lys Glu Met
450 455 460
Glu Pro Tyr Val Ala Asn Ser Pro Arg Thr Ala Tyr Leu Asn Tyr Arg
465 470 475 480
Asp Leu Asp Phe Gly Ser Asn Asp Asp Asn Tyr Ser Tyr Ser Lys Ala
485 490 495
Lys Ile Trp Gly Glu Lys Tyr Phe Asn Tyr Asn Phe Glu Arg Leu Ala
500 505 510
Lys Val Lys Ser Lys Val Asp Pro Asn Asn Phe Phe Arg Thr Glu Gln
515 520 525
Ser Ile Pro Leu Tyr Gln Thr Tyr
530 535
<210> 88
<211> 1551
<212> DNA
<213> 人工序列
<220>
<223> CBS124-A0A1U8FGM5-无信号肽
<220>
<221> CDS
<222> (1)..(1551)
<400> 88
atg tcc aga gaa gat ttc ttg aga tgc ttg ttc aag tcc tcc gaa tct 48
Met Ser Arg Glu Asp Phe Leu Arg Cys Leu Phe Lys Ser Ser Glu Ser
1 5 10 15
aac gtt act caa aac atc tac acc cca aac tca ttg acc tac tcc tct 96
Asn Val Thr Gln Asn Ile Tyr Thr Pro Asn Ser Leu Thr Tyr Ser Ser
20 25 30
att ttg aag tac gct caa aaa cac tcc aga tgg gtt aat tct tct cag 144
Ile Leu Lys Tyr Ala Gln Lys His Ser Arg Trp Val Asn Ser Ser Gln
35 40 45
cca aat ttc att gct tct ccc aca aaa gaa tcc gaa atc aag cca gtt 192
Pro Asn Phe Ile Ala Ser Pro Thr Lys Glu Ser Glu Ile Lys Pro Val
50 55 60
att ttg tgc gct aag aag ttg ggt ctg caa atc aaa atc aaa tcc ggt 240
Ile Leu Cys Ala Lys Lys Leu Gly Leu Gln Ile Lys Ile Lys Ser Gly
65 70 75 80
ggt cat gat tac gag ggt att tct tac aga tct gaa ttc cca ttc gtc 288
Gly His Asp Tyr Glu Gly Ile Ser Tyr Arg Ser Glu Phe Pro Phe Val
85 90 95
atg ttg gac ttg tcc aat ttg aac aag acc gaa atc gac ttg aac gaa 336
Met Leu Asp Leu Ser Asn Leu Asn Lys Thr Glu Ile Asp Leu Asn Glu
100 105 110
gaa acc att tgg gtt caa gct ggt gct act ttg ggt caa cta tat tac 384
Glu Thr Ile Trp Val Gln Ala Gly Ala Thr Leu Gly Gln Leu Tyr Tyr
115 120 125
gct att gcc aac aga tcc aac gtt cat ggt ttt cca ggt ggt att tgt 432
Ala Ile Ala Asn Arg Ser Asn Val His Gly Phe Pro Gly Gly Ile Cys
130 135 140
ttc tct gtt ggt act ggt ggt ttg att tct ggt ggt ggt tta ggt act 480
Phe Ser Val Gly Thr Gly Gly Leu Ile Ser Gly Gly Gly Leu Gly Thr
145 150 155 160
atg atg aga aaa ttt ggt ttg gcc gct gat aac gtt gtt gat gct aga 528
Met Met Arg Lys Phe Gly Leu Ala Ala Asp Asn Val Val Asp Ala Arg
165 170 175
gtt atg gat gtc aac ggt aac atc ttg gac cgt aaa aag atg aag aac 576
Val Met Asp Val Asn Gly Asn Ile Leu Asp Arg Lys Lys Met Lys Asn
180 185 190
gat ttg ttc tgg gca att aga ggt ggt ggt ggc gct tct ttt ggt gtt 624
Asp Leu Phe Trp Ala Ile Arg Gly Gly Gly Gly Ala Ser Phe Gly Val
195 200 205
att ttg gct tgg aaa ttg aag ttg gtt agg gtt cca gaa aag gtt act 672
Ile Leu Ala Trp Lys Leu Lys Leu Val Arg Val Pro Glu Lys Val Thr
210 215 220
gtt ttc acc gtc tgc aaa aag ttc gat gat aac cag aac ttg atc ctg 720
Val Phe Thr Val Cys Lys Lys Phe Asp Asp Asn Gln Asn Leu Ile Leu
225 230 235 240
aag tgg gaa aac att gct cat caa ttg cca gat aag ttg gtc atc aga 768
Lys Trp Glu Asn Ile Ala His Gln Leu Pro Asp Lys Leu Val Ile Arg
245 250 255
gtt gtt atc caa aac gat ggt aca ggt aac gat aag tac gtc gaa atg 816
Val Val Ile Gln Asn Asp Gly Thr Gly Asn Asp Lys Tyr Val Glu Met
260 265 270
aga ttt caa gcc ttg tat ttg ggt cca gtt gat gaa ttg atc ccc ttg 864
Arg Phe Gln Ala Leu Tyr Leu Gly Pro Val Asp Glu Leu Ile Pro Leu
275 280 285
ttg aaa gaa aag ttc cca gaa ttc gac ttg gaa aag aag gat tgc ttg 912
Leu Lys Glu Lys Phe Pro Glu Phe Asp Leu Glu Lys Lys Asp Cys Leu
290 295 300
caa gaa cca gtt ggt gat aga tct aac aga tcc tgc att aac aaa gaa 960
Gln Glu Pro Val Gly Asp Arg Ser Asn Arg Ser Cys Ile Asn Lys Glu
305 310 315 320
tgc aga gaa acc tct tgg atc ggt tct gtc ttg tac ttt tac gaa aga 1008
Cys Arg Glu Thr Ser Trp Ile Gly Ser Val Leu Tyr Phe Tyr Glu Arg
325 330 335
gaa acc acc tcc tca ttg gag gtc tta ttg aaa gaa tcc att cca aag 1056
Glu Thr Thr Ser Ser Leu Glu Val Leu Leu Lys Glu Ser Ile Pro Lys
340 345 350
cag aag tcc tac tac aaa ggt act tct gat ttc gtc aag acc cag att 1104
Gln Lys Ser Tyr Tyr Lys Gly Thr Ser Asp Phe Val Lys Thr Gln Ile
355 360 365
tct gaa tct ggt tgg gaa atg gtt gaa agg ttg ttg ttg gaa gaa gaa 1152
Ser Glu Ser Gly Trp Glu Met Val Glu Arg Leu Leu Leu Glu Glu Glu
370 375 380
tcc cca aga att atc ttg gaa cca tta ggt ggt aag ttg gac gaa atc 1200
Ser Pro Arg Ile Ile Leu Glu Pro Leu Gly Gly Lys Leu Asp Glu Ile
385 390 395 400
tcc gaa tca gaa att cca ttt cca cac aga aaa ggc aac ttg tac aac 1248
Ser Glu Ser Glu Ile Pro Phe Pro His Arg Lys Gly Asn Leu Tyr Asn
405 410 415
atc caa tac ttg gtt tac tgg cac gac aac aac gaa aac gtt tcc tct 1296
Ile Gln Tyr Leu Val Tyr Trp His Asp Asn Asn Glu Asn Val Ser Ser
420 425 430
caa aaa att gcc tgg atg cgt aag ctg tac aaa gaa atg gaa cca tac 1344
Gln Lys Ile Ala Trp Met Arg Lys Leu Tyr Lys Glu Met Glu Pro Tyr
435 440 445
gtt gct aac tct cca aga act gct tac ttg aac tac aga gat ttg gac 1392
Val Ala Asn Ser Pro Arg Thr Ala Tyr Leu Asn Tyr Arg Asp Leu Asp
450 455 460
ttc ggt tct aac gat gac aac tac tct tac tcc aaa gct aaa atc tgg 1440
Phe Gly Ser Asn Asp Asp Asn Tyr Ser Tyr Ser Lys Ala Lys Ile Trp
465 470 475 480
ggc gaa aag tac ttc aac tac aac ttt gaa aga ctg gcc aag gtt aag 1488
Gly Glu Lys Tyr Phe Asn Tyr Asn Phe Glu Arg Leu Ala Lys Val Lys
485 490 495
tct aag gtt gat cca aac aac ttc ttc aga acc gaa caa tct att ccc 1536
Ser Lys Val Asp Pro Asn Asn Phe Phe Arg Thr Glu Gln Ser Ile Pro
500 505 510
ttg tac caa acc tac 1551
Leu Tyr Gln Thr Tyr
515
<210> 89
<211> 517
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 89
Met Ser Arg Glu Asp Phe Leu Arg Cys Leu Phe Lys Ser Ser Glu Ser
1 5 10 15
Asn Val Thr Gln Asn Ile Tyr Thr Pro Asn Ser Leu Thr Tyr Ser Ser
20 25 30
Ile Leu Lys Tyr Ala Gln Lys His Ser Arg Trp Val Asn Ser Ser Gln
35 40 45
Pro Asn Phe Ile Ala Ser Pro Thr Lys Glu Ser Glu Ile Lys Pro Val
50 55 60
Ile Leu Cys Ala Lys Lys Leu Gly Leu Gln Ile Lys Ile Lys Ser Gly
65 70 75 80
Gly His Asp Tyr Glu Gly Ile Ser Tyr Arg Ser Glu Phe Pro Phe Val
85 90 95
Met Leu Asp Leu Ser Asn Leu Asn Lys Thr Glu Ile Asp Leu Asn Glu
100 105 110
Glu Thr Ile Trp Val Gln Ala Gly Ala Thr Leu Gly Gln Leu Tyr Tyr
115 120 125
Ala Ile Ala Asn Arg Ser Asn Val His Gly Phe Pro Gly Gly Ile Cys
130 135 140
Phe Ser Val Gly Thr Gly Gly Leu Ile Ser Gly Gly Gly Leu Gly Thr
145 150 155 160
Met Met Arg Lys Phe Gly Leu Ala Ala Asp Asn Val Val Asp Ala Arg
165 170 175
Val Met Asp Val Asn Gly Asn Ile Leu Asp Arg Lys Lys Met Lys Asn
180 185 190
Asp Leu Phe Trp Ala Ile Arg Gly Gly Gly Gly Ala Ser Phe Gly Val
195 200 205
Ile Leu Ala Trp Lys Leu Lys Leu Val Arg Val Pro Glu Lys Val Thr
210 215 220
Val Phe Thr Val Cys Lys Lys Phe Asp Asp Asn Gln Asn Leu Ile Leu
225 230 235 240
Lys Trp Glu Asn Ile Ala His Gln Leu Pro Asp Lys Leu Val Ile Arg
245 250 255
Val Val Ile Gln Asn Asp Gly Thr Gly Asn Asp Lys Tyr Val Glu Met
260 265 270
Arg Phe Gln Ala Leu Tyr Leu Gly Pro Val Asp Glu Leu Ile Pro Leu
275 280 285
Leu Lys Glu Lys Phe Pro Glu Phe Asp Leu Glu Lys Lys Asp Cys Leu
290 295 300
Gln Glu Pro Val Gly Asp Arg Ser Asn Arg Ser Cys Ile Asn Lys Glu
305 310 315 320
Cys Arg Glu Thr Ser Trp Ile Gly Ser Val Leu Tyr Phe Tyr Glu Arg
325 330 335
Glu Thr Thr Ser Ser Leu Glu Val Leu Leu Lys Glu Ser Ile Pro Lys
340 345 350
Gln Lys Ser Tyr Tyr Lys Gly Thr Ser Asp Phe Val Lys Thr Gln Ile
355 360 365
Ser Glu Ser Gly Trp Glu Met Val Glu Arg Leu Leu Leu Glu Glu Glu
370 375 380
Ser Pro Arg Ile Ile Leu Glu Pro Leu Gly Gly Lys Leu Asp Glu Ile
385 390 395 400
Ser Glu Ser Glu Ile Pro Phe Pro His Arg Lys Gly Asn Leu Tyr Asn
405 410 415
Ile Gln Tyr Leu Val Tyr Trp His Asp Asn Asn Glu Asn Val Ser Ser
420 425 430
Gln Lys Ile Ala Trp Met Arg Lys Leu Tyr Lys Glu Met Glu Pro Tyr
435 440 445
Val Ala Asn Ser Pro Arg Thr Ala Tyr Leu Asn Tyr Arg Asp Leu Asp
450 455 460
Phe Gly Ser Asn Asp Asp Asn Tyr Ser Tyr Ser Lys Ala Lys Ile Trp
465 470 475 480
Gly Glu Lys Tyr Phe Asn Tyr Asn Phe Glu Arg Leu Ala Lys Val Lys
485 490 495
Ser Lys Val Asp Pro Asn Asn Phe Phe Arg Thr Glu Gln Ser Ile Pro
500 505 510
Leu Tyr Gln Thr Tyr
515
<210> 90
<211> 1575
<212> DNA
<213> 人工序列
<220>
<223> CBS124-A0A1U8FGM5-无信号肽-strep标签
<220>
<221> CDS
<222> (1)..(1575)
<400> 90
atg tcc aga gaa gat ttc ttg aga tgc ttg ttc aag tcc tcc gaa tct 48
Met Ser Arg Glu Asp Phe Leu Arg Cys Leu Phe Lys Ser Ser Glu Ser
1 5 10 15
aac gtt act caa aac atc tac acc cca aac tca ttg acc tac tcc tct 96
Asn Val Thr Gln Asn Ile Tyr Thr Pro Asn Ser Leu Thr Tyr Ser Ser
20 25 30
att ttg aag tac gct caa aaa cac tcc aga tgg gtt aat tct tct cag 144
Ile Leu Lys Tyr Ala Gln Lys His Ser Arg Trp Val Asn Ser Ser Gln
35 40 45
cca aat ttc att gct tct ccc aca aaa gaa tcc gaa atc aag cca gtt 192
Pro Asn Phe Ile Ala Ser Pro Thr Lys Glu Ser Glu Ile Lys Pro Val
50 55 60
att ttg tgc gct aag aag ttg ggt ctg caa atc aaa atc aaa tcc ggt 240
Ile Leu Cys Ala Lys Lys Leu Gly Leu Gln Ile Lys Ile Lys Ser Gly
65 70 75 80
ggt cat gat tac gag ggt att tct tac aga tct gaa ttc cca ttc gtc 288
Gly His Asp Tyr Glu Gly Ile Ser Tyr Arg Ser Glu Phe Pro Phe Val
85 90 95
atg ttg gac ttg tcc aat ttg aac aag acc gaa atc gac ttg aac gaa 336
Met Leu Asp Leu Ser Asn Leu Asn Lys Thr Glu Ile Asp Leu Asn Glu
100 105 110
gaa acc att tgg gtt caa gct ggt gct act ttg ggt caa cta tat tac 384
Glu Thr Ile Trp Val Gln Ala Gly Ala Thr Leu Gly Gln Leu Tyr Tyr
115 120 125
gct att gcc aac aga tcc aac gtt cat ggt ttt cca ggt ggt att tgt 432
Ala Ile Ala Asn Arg Ser Asn Val His Gly Phe Pro Gly Gly Ile Cys
130 135 140
ttc tct gtt ggt act ggt ggt ttg att tct ggt ggt ggt tta ggt act 480
Phe Ser Val Gly Thr Gly Gly Leu Ile Ser Gly Gly Gly Leu Gly Thr
145 150 155 160
atg atg aga aaa ttt ggt ttg gcc gct gat aac gtt gtt gat gct aga 528
Met Met Arg Lys Phe Gly Leu Ala Ala Asp Asn Val Val Asp Ala Arg
165 170 175
gtt atg gat gtc aac ggt aac atc ttg gac cgt aaa aag atg aag aac 576
Val Met Asp Val Asn Gly Asn Ile Leu Asp Arg Lys Lys Met Lys Asn
180 185 190
gat ttg ttc tgg gca att aga ggt ggt ggt ggc gct tct ttt ggt gtt 624
Asp Leu Phe Trp Ala Ile Arg Gly Gly Gly Gly Ala Ser Phe Gly Val
195 200 205
att ttg gct tgg aaa ttg aag ttg gtt agg gtt cca gaa aag gtt act 672
Ile Leu Ala Trp Lys Leu Lys Leu Val Arg Val Pro Glu Lys Val Thr
210 215 220
gtt ttc acc gtc tgc aaa aag ttc gat gat aac cag aac ttg atc ctg 720
Val Phe Thr Val Cys Lys Lys Phe Asp Asp Asn Gln Asn Leu Ile Leu
225 230 235 240
aag tgg gaa aac att gct cat caa ttg cca gat aag ttg gtc atc aga 768
Lys Trp Glu Asn Ile Ala His Gln Leu Pro Asp Lys Leu Val Ile Arg
245 250 255
gtt gtt atc caa aac gat ggt aca ggt aac gat aag tac gtc gaa atg 816
Val Val Ile Gln Asn Asp Gly Thr Gly Asn Asp Lys Tyr Val Glu Met
260 265 270
aga ttt caa gcc ttg tat ttg ggt cca gtt gat gaa ttg atc ccc ttg 864
Arg Phe Gln Ala Leu Tyr Leu Gly Pro Val Asp Glu Leu Ile Pro Leu
275 280 285
ttg aaa gaa aag ttc cca gaa ttc gac ttg gaa aag aag gat tgc ttg 912
Leu Lys Glu Lys Phe Pro Glu Phe Asp Leu Glu Lys Lys Asp Cys Leu
290 295 300
caa gaa cca gtt ggt gat aga tct aac aga tcc tgc att aac aaa gaa 960
Gln Glu Pro Val Gly Asp Arg Ser Asn Arg Ser Cys Ile Asn Lys Glu
305 310 315 320
tgc aga gaa acc tct tgg atc ggt tct gtc ttg tac ttt tac gaa aga 1008
Cys Arg Glu Thr Ser Trp Ile Gly Ser Val Leu Tyr Phe Tyr Glu Arg
325 330 335
gaa acc acc tcc tca ttg gag gtc tta ttg aaa gaa tcc att cca aag 1056
Glu Thr Thr Ser Ser Leu Glu Val Leu Leu Lys Glu Ser Ile Pro Lys
340 345 350
cag aag tcc tac tac aaa ggt act tct gat ttc gtc aag acc cag att 1104
Gln Lys Ser Tyr Tyr Lys Gly Thr Ser Asp Phe Val Lys Thr Gln Ile
355 360 365
tct gaa tct ggt tgg gaa atg gtt gaa agg ttg ttg ttg gaa gaa gaa 1152
Ser Glu Ser Gly Trp Glu Met Val Glu Arg Leu Leu Leu Glu Glu Glu
370 375 380
tcc cca aga att atc ttg gaa cca tta ggt ggt aag ttg gac gaa atc 1200
Ser Pro Arg Ile Ile Leu Glu Pro Leu Gly Gly Lys Leu Asp Glu Ile
385 390 395 400
tcc gaa tca gaa att cca ttt cca cac aga aaa ggc aac ttg tac aac 1248
Ser Glu Ser Glu Ile Pro Phe Pro His Arg Lys Gly Asn Leu Tyr Asn
405 410 415
atc caa tac ttg gtt tac tgg cac gac aac aac gaa aac gtt tcc tct 1296
Ile Gln Tyr Leu Val Tyr Trp His Asp Asn Asn Glu Asn Val Ser Ser
420 425 430
caa aaa att gcc tgg atg cgt aag ctg tac aaa gaa atg gaa cca tac 1344
Gln Lys Ile Ala Trp Met Arg Lys Leu Tyr Lys Glu Met Glu Pro Tyr
435 440 445
gtt gct aac tct cca aga act gct tac ttg aac tac aga gat ttg gac 1392
Val Ala Asn Ser Pro Arg Thr Ala Tyr Leu Asn Tyr Arg Asp Leu Asp
450 455 460
ttc ggt tct aac gat gac aac tac tct tac tcc aaa gct aaa atc tgg 1440
Phe Gly Ser Asn Asp Asp Asn Tyr Ser Tyr Ser Lys Ala Lys Ile Trp
465 470 475 480
ggc gaa aag tac ttc aac tac aac ttt gaa aga ctg gcc aag gtt aag 1488
Gly Glu Lys Tyr Phe Asn Tyr Asn Phe Glu Arg Leu Ala Lys Val Lys
485 490 495
tct aag gtt gat cca aac aac ttc ttc aga acc gaa caa tct att ccc 1536
Ser Lys Val Asp Pro Asn Asn Phe Phe Arg Thr Glu Gln Ser Ile Pro
500 505 510
ttg tac caa acc tac tgg tca cat cca caa ttt gaa aag 1575
Leu Tyr Gln Thr Tyr Trp Ser His Pro Gln Phe Glu Lys
515 520 525
<210> 91
<211> 525
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 91
Met Ser Arg Glu Asp Phe Leu Arg Cys Leu Phe Lys Ser Ser Glu Ser
1 5 10 15
Asn Val Thr Gln Asn Ile Tyr Thr Pro Asn Ser Leu Thr Tyr Ser Ser
20 25 30
Ile Leu Lys Tyr Ala Gln Lys His Ser Arg Trp Val Asn Ser Ser Gln
35 40 45
Pro Asn Phe Ile Ala Ser Pro Thr Lys Glu Ser Glu Ile Lys Pro Val
50 55 60
Ile Leu Cys Ala Lys Lys Leu Gly Leu Gln Ile Lys Ile Lys Ser Gly
65 70 75 80
Gly His Asp Tyr Glu Gly Ile Ser Tyr Arg Ser Glu Phe Pro Phe Val
85 90 95
Met Leu Asp Leu Ser Asn Leu Asn Lys Thr Glu Ile Asp Leu Asn Glu
100 105 110
Glu Thr Ile Trp Val Gln Ala Gly Ala Thr Leu Gly Gln Leu Tyr Tyr
115 120 125
Ala Ile Ala Asn Arg Ser Asn Val His Gly Phe Pro Gly Gly Ile Cys
130 135 140
Phe Ser Val Gly Thr Gly Gly Leu Ile Ser Gly Gly Gly Leu Gly Thr
145 150 155 160
Met Met Arg Lys Phe Gly Leu Ala Ala Asp Asn Val Val Asp Ala Arg
165 170 175
Val Met Asp Val Asn Gly Asn Ile Leu Asp Arg Lys Lys Met Lys Asn
180 185 190
Asp Leu Phe Trp Ala Ile Arg Gly Gly Gly Gly Ala Ser Phe Gly Val
195 200 205
Ile Leu Ala Trp Lys Leu Lys Leu Val Arg Val Pro Glu Lys Val Thr
210 215 220
Val Phe Thr Val Cys Lys Lys Phe Asp Asp Asn Gln Asn Leu Ile Leu
225 230 235 240
Lys Trp Glu Asn Ile Ala His Gln Leu Pro Asp Lys Leu Val Ile Arg
245 250 255
Val Val Ile Gln Asn Asp Gly Thr Gly Asn Asp Lys Tyr Val Glu Met
260 265 270
Arg Phe Gln Ala Leu Tyr Leu Gly Pro Val Asp Glu Leu Ile Pro Leu
275 280 285
Leu Lys Glu Lys Phe Pro Glu Phe Asp Leu Glu Lys Lys Asp Cys Leu
290 295 300
Gln Glu Pro Val Gly Asp Arg Ser Asn Arg Ser Cys Ile Asn Lys Glu
305 310 315 320
Cys Arg Glu Thr Ser Trp Ile Gly Ser Val Leu Tyr Phe Tyr Glu Arg
325 330 335
Glu Thr Thr Ser Ser Leu Glu Val Leu Leu Lys Glu Ser Ile Pro Lys
340 345 350
Gln Lys Ser Tyr Tyr Lys Gly Thr Ser Asp Phe Val Lys Thr Gln Ile
355 360 365
Ser Glu Ser Gly Trp Glu Met Val Glu Arg Leu Leu Leu Glu Glu Glu
370 375 380
Ser Pro Arg Ile Ile Leu Glu Pro Leu Gly Gly Lys Leu Asp Glu Ile
385 390 395 400
Ser Glu Ser Glu Ile Pro Phe Pro His Arg Lys Gly Asn Leu Tyr Asn
405 410 415
Ile Gln Tyr Leu Val Tyr Trp His Asp Asn Asn Glu Asn Val Ser Ser
420 425 430
Gln Lys Ile Ala Trp Met Arg Lys Leu Tyr Lys Glu Met Glu Pro Tyr
435 440 445
Val Ala Asn Ser Pro Arg Thr Ala Tyr Leu Asn Tyr Arg Asp Leu Asp
450 455 460
Phe Gly Ser Asn Asp Asp Asn Tyr Ser Tyr Ser Lys Ala Lys Ile Trp
465 470 475 480
Gly Glu Lys Tyr Phe Asn Tyr Asn Phe Glu Arg Leu Ala Lys Val Lys
485 490 495
Ser Lys Val Asp Pro Asn Asn Phe Phe Arg Thr Glu Gln Ser Ile Pro
500 505 510
Leu Tyr Gln Thr Tyr Trp Ser His Pro Gln Phe Glu Lys
515 520 525
<210> 92
<211> 1632
<212> DNA
<213> 人工序列
<220>
<223> CBS124-A0A1U8FGM5-截断alpha因子-无信号肽-strep标签
<220>
<221> CDS
<222> (1)..(1632)
<400> 92
atg aga ttt cct tcc atc ttc acg gct gtg cta ttt gca gca tcc tcc 48
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser
1 5 10 15
gca ctt gca atg tcc aga gaa gat ttc ttg aga tgc ttg ttc aag tcc 96
Ala Leu Ala Met Ser Arg Glu Asp Phe Leu Arg Cys Leu Phe Lys Ser
20 25 30
tcc gaa tct aac gtt act caa aac atc tac acc cca aac tca ttg acc 144
Ser Glu Ser Asn Val Thr Gln Asn Ile Tyr Thr Pro Asn Ser Leu Thr
35 40 45
tac tcc tct att ttg aag tac gct caa aaa cac tcc aga tgg gtt aat 192
Tyr Ser Ser Ile Leu Lys Tyr Ala Gln Lys His Ser Arg Trp Val Asn
50 55 60
tct tct cag cca aat ttc att gct tct ccc aca aaa gaa tcc gaa atc 240
Ser Ser Gln Pro Asn Phe Ile Ala Ser Pro Thr Lys Glu Ser Glu Ile
65 70 75 80
aag cca gtt att ttg tgc gct aag aag ttg ggt ctg caa atc aaa atc 288
Lys Pro Val Ile Leu Cys Ala Lys Lys Leu Gly Leu Gln Ile Lys Ile
85 90 95
aaa tcc ggt ggt cat gat tac gag ggt att tct tac aga tct gaa ttc 336
Lys Ser Gly Gly His Asp Tyr Glu Gly Ile Ser Tyr Arg Ser Glu Phe
100 105 110
cca ttc gtc atg ttg gac ttg tcc aat ttg aac aag acc gaa atc gac 384
Pro Phe Val Met Leu Asp Leu Ser Asn Leu Asn Lys Thr Glu Ile Asp
115 120 125
ttg aac gaa gaa acc att tgg gtt caa gct ggt gct act ttg ggt caa 432
Leu Asn Glu Glu Thr Ile Trp Val Gln Ala Gly Ala Thr Leu Gly Gln
130 135 140
cta tat tac gct att gcc aac aga tcc aac gtt cat ggt ttt cca ggt 480
Leu Tyr Tyr Ala Ile Ala Asn Arg Ser Asn Val His Gly Phe Pro Gly
145 150 155 160
ggt att tgt ttc tct gtt ggt act ggt ggt ttg att tct ggt ggt ggt 528
Gly Ile Cys Phe Ser Val Gly Thr Gly Gly Leu Ile Ser Gly Gly Gly
165 170 175
tta ggt act atg atg aga aaa ttt ggt ttg gcc gct gat aac gtt gtt 576
Leu Gly Thr Met Met Arg Lys Phe Gly Leu Ala Ala Asp Asn Val Val
180 185 190
gat gct aga gtt atg gat gtc aac ggt aac atc ttg gac cgt aaa aag 624
Asp Ala Arg Val Met Asp Val Asn Gly Asn Ile Leu Asp Arg Lys Lys
195 200 205
atg aag aac gat ttg ttc tgg gca att aga ggt ggt ggt ggc gct tct 672
Met Lys Asn Asp Leu Phe Trp Ala Ile Arg Gly Gly Gly Gly Ala Ser
210 215 220
ttt ggt gtt att ttg gct tgg aaa ttg aag ttg gtt agg gtt cca gaa 720
Phe Gly Val Ile Leu Ala Trp Lys Leu Lys Leu Val Arg Val Pro Glu
225 230 235 240
aag gtt act gtt ttc acc gtc tgc aaa aag ttc gat gat aac cag aac 768
Lys Val Thr Val Phe Thr Val Cys Lys Lys Phe Asp Asp Asn Gln Asn
245 250 255
ttg atc ctg aag tgg gaa aac att gct cat caa ttg cca gat aag ttg 816
Leu Ile Leu Lys Trp Glu Asn Ile Ala His Gln Leu Pro Asp Lys Leu
260 265 270
gtc atc aga gtt gtt atc caa aac gat ggt aca ggt aac gat aag tac 864
Val Ile Arg Val Val Ile Gln Asn Asp Gly Thr Gly Asn Asp Lys Tyr
275 280 285
gtc gaa atg aga ttt caa gcc ttg tat ttg ggt cca gtt gat gaa ttg 912
Val Glu Met Arg Phe Gln Ala Leu Tyr Leu Gly Pro Val Asp Glu Leu
290 295 300
atc ccc ttg ttg aaa gaa aag ttc cca gaa ttc gac ttg gaa aag aag 960
Ile Pro Leu Leu Lys Glu Lys Phe Pro Glu Phe Asp Leu Glu Lys Lys
305 310 315 320
gat tgc ttg caa gaa cca gtt ggt gat aga tct aac aga tcc tgc att 1008
Asp Cys Leu Gln Glu Pro Val Gly Asp Arg Ser Asn Arg Ser Cys Ile
325 330 335
aac aaa gaa tgc aga gaa acc tct tgg atc ggt tct gtc ttg tac ttt 1056
Asn Lys Glu Cys Arg Glu Thr Ser Trp Ile Gly Ser Val Leu Tyr Phe
340 345 350
tac gaa aga gaa acc acc tcc tca ttg gag gtc tta ttg aaa gaa tcc 1104
Tyr Glu Arg Glu Thr Thr Ser Ser Leu Glu Val Leu Leu Lys Glu Ser
355 360 365
att cca aag cag aag tcc tac tac aaa ggt act tct gat ttc gtc aag 1152
Ile Pro Lys Gln Lys Ser Tyr Tyr Lys Gly Thr Ser Asp Phe Val Lys
370 375 380
acc cag att tct gaa tct ggt tgg gaa atg gtt gaa agg ttg ttg ttg 1200
Thr Gln Ile Ser Glu Ser Gly Trp Glu Met Val Glu Arg Leu Leu Leu
385 390 395 400
gaa gaa gaa tcc cca aga att atc ttg gaa cca tta ggt ggt aag ttg 1248
Glu Glu Glu Ser Pro Arg Ile Ile Leu Glu Pro Leu Gly Gly Lys Leu
405 410 415
gac gaa atc tcc gaa tca gaa att cca ttt cca cac aga aaa ggc aac 1296
Asp Glu Ile Ser Glu Ser Glu Ile Pro Phe Pro His Arg Lys Gly Asn
420 425 430
ttg tac aac atc caa tac ttg gtt tac tgg cac gac aac aac gaa aac 1344
Leu Tyr Asn Ile Gln Tyr Leu Val Tyr Trp His Asp Asn Asn Glu Asn
435 440 445
gtt tcc tct caa aaa att gcc tgg atg cgt aag ctg tac aaa gaa atg 1392
Val Ser Ser Gln Lys Ile Ala Trp Met Arg Lys Leu Tyr Lys Glu Met
450 455 460
gaa cca tac gtt gct aac tct cca aga act gct tac ttg aac tac aga 1440
Glu Pro Tyr Val Ala Asn Ser Pro Arg Thr Ala Tyr Leu Asn Tyr Arg
465 470 475 480
gat ttg gac ttc ggt tct aac gat gac aac tac tct tac tcc aaa gct 1488
Asp Leu Asp Phe Gly Ser Asn Asp Asp Asn Tyr Ser Tyr Ser Lys Ala
485 490 495
aaa atc tgg ggc gaa aag tac ttc aac tac aac ttt gaa aga ctg gcc 1536
Lys Ile Trp Gly Glu Lys Tyr Phe Asn Tyr Asn Phe Glu Arg Leu Ala
500 505 510
aag gtt aag tct aag gtt gat cca aac aac ttc ttc aga acc gaa caa 1584
Lys Val Lys Ser Lys Val Asp Pro Asn Asn Phe Phe Arg Thr Glu Gln
515 520 525
tct att ccc ttg tac caa acc tac tgg tca cat cca caa ttt gaa aag 1632
Ser Ile Pro Leu Tyr Gln Thr Tyr Trp Ser His Pro Gln Phe Glu Lys
530 535 540
<210> 93
<211> 544
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 93
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser
1 5 10 15
Ala Leu Ala Met Ser Arg Glu Asp Phe Leu Arg Cys Leu Phe Lys Ser
20 25 30
Ser Glu Ser Asn Val Thr Gln Asn Ile Tyr Thr Pro Asn Ser Leu Thr
35 40 45
Tyr Ser Ser Ile Leu Lys Tyr Ala Gln Lys His Ser Arg Trp Val Asn
50 55 60
Ser Ser Gln Pro Asn Phe Ile Ala Ser Pro Thr Lys Glu Ser Glu Ile
65 70 75 80
Lys Pro Val Ile Leu Cys Ala Lys Lys Leu Gly Leu Gln Ile Lys Ile
85 90 95
Lys Ser Gly Gly His Asp Tyr Glu Gly Ile Ser Tyr Arg Ser Glu Phe
100 105 110
Pro Phe Val Met Leu Asp Leu Ser Asn Leu Asn Lys Thr Glu Ile Asp
115 120 125
Leu Asn Glu Glu Thr Ile Trp Val Gln Ala Gly Ala Thr Leu Gly Gln
130 135 140
Leu Tyr Tyr Ala Ile Ala Asn Arg Ser Asn Val His Gly Phe Pro Gly
145 150 155 160
Gly Ile Cys Phe Ser Val Gly Thr Gly Gly Leu Ile Ser Gly Gly Gly
165 170 175
Leu Gly Thr Met Met Arg Lys Phe Gly Leu Ala Ala Asp Asn Val Val
180 185 190
Asp Ala Arg Val Met Asp Val Asn Gly Asn Ile Leu Asp Arg Lys Lys
195 200 205
Met Lys Asn Asp Leu Phe Trp Ala Ile Arg Gly Gly Gly Gly Ala Ser
210 215 220
Phe Gly Val Ile Leu Ala Trp Lys Leu Lys Leu Val Arg Val Pro Glu
225 230 235 240
Lys Val Thr Val Phe Thr Val Cys Lys Lys Phe Asp Asp Asn Gln Asn
245 250 255
Leu Ile Leu Lys Trp Glu Asn Ile Ala His Gln Leu Pro Asp Lys Leu
260 265 270
Val Ile Arg Val Val Ile Gln Asn Asp Gly Thr Gly Asn Asp Lys Tyr
275 280 285
Val Glu Met Arg Phe Gln Ala Leu Tyr Leu Gly Pro Val Asp Glu Leu
290 295 300
Ile Pro Leu Leu Lys Glu Lys Phe Pro Glu Phe Asp Leu Glu Lys Lys
305 310 315 320
Asp Cys Leu Gln Glu Pro Val Gly Asp Arg Ser Asn Arg Ser Cys Ile
325 330 335
Asn Lys Glu Cys Arg Glu Thr Ser Trp Ile Gly Ser Val Leu Tyr Phe
340 345 350
Tyr Glu Arg Glu Thr Thr Ser Ser Leu Glu Val Leu Leu Lys Glu Ser
355 360 365
Ile Pro Lys Gln Lys Ser Tyr Tyr Lys Gly Thr Ser Asp Phe Val Lys
370 375 380
Thr Gln Ile Ser Glu Ser Gly Trp Glu Met Val Glu Arg Leu Leu Leu
385 390 395 400
Glu Glu Glu Ser Pro Arg Ile Ile Leu Glu Pro Leu Gly Gly Lys Leu
405 410 415
Asp Glu Ile Ser Glu Ser Glu Ile Pro Phe Pro His Arg Lys Gly Asn
420 425 430
Leu Tyr Asn Ile Gln Tyr Leu Val Tyr Trp His Asp Asn Asn Glu Asn
435 440 445
Val Ser Ser Gln Lys Ile Ala Trp Met Arg Lys Leu Tyr Lys Glu Met
450 455 460
Glu Pro Tyr Val Ala Asn Ser Pro Arg Thr Ala Tyr Leu Asn Tyr Arg
465 470 475 480
Asp Leu Asp Phe Gly Ser Asn Asp Asp Asn Tyr Ser Tyr Ser Lys Ala
485 490 495
Lys Ile Trp Gly Glu Lys Tyr Phe Asn Tyr Asn Phe Glu Arg Leu Ala
500 505 510
Lys Val Lys Ser Lys Val Asp Pro Asn Asn Phe Phe Arg Thr Glu Gln
515 520 525
Ser Ile Pro Leu Tyr Gln Thr Tyr Trp Ser His Pro Gln Phe Glu Lys
530 535 540
<210> 94
<211> 1842
<212> DNA
<213> 人工序列
<220>
<223> CBS124-A0A1U8FGM5-全长alpha因子-无信号肽-strep标签
<220>
<221> CDS
<222> (1)..(1842)
<400> 94
atg aga ttt cct tca att ttt act gct gtt tta ttc gca gca tcc tcc 48
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser
1 5 10 15
gca tta gct gct cca gtc aac act aca aca gaa gat gaa acg gca caa 96
Ala Leu Ala Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln
20 25 30
att ccg gct gaa gct gtc atc ggt tac tca gat tta gaa ggg gat ttc 144
Ile Pro Ala Glu Ala Val Ile Gly Tyr Ser Asp Leu Glu Gly Asp Phe
35 40 45
gat gtt gct gtt ttg cca ttt tcc aac agc aca aat aac ggg tta ttg 192
Asp Val Ala Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu
50 55 60
ttt ata aat act act att gcc agc att gct gct aaa gaa gaa ggg gta 240
Phe Ile Asn Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val
65 70 75 80
tct ctc gag aaa aga gag gct gaa gct atg tcc aga gaa gat ttc ttg 288
Ser Leu Glu Lys Arg Glu Ala Glu Ala Met Ser Arg Glu Asp Phe Leu
85 90 95
aga tgc ttg ttc aag tcc tcc gaa tct aac gtt act caa aac atc tac 336
Arg Cys Leu Phe Lys Ser Ser Glu Ser Asn Val Thr Gln Asn Ile Tyr
100 105 110
acc cca aac tca ttg acc tac tcc tct att ttg aag tac gct caa aaa 384
Thr Pro Asn Ser Leu Thr Tyr Ser Ser Ile Leu Lys Tyr Ala Gln Lys
115 120 125
cac tcc aga tgg gtt aat tct tct cag cca aat ttc att gct tct ccc 432
His Ser Arg Trp Val Asn Ser Ser Gln Pro Asn Phe Ile Ala Ser Pro
130 135 140
aca aaa gaa tcc gaa atc aag cca gtt att ttg tgc gct aag aag ttg 480
Thr Lys Glu Ser Glu Ile Lys Pro Val Ile Leu Cys Ala Lys Lys Leu
145 150 155 160
ggt ctg caa atc aaa atc aaa tcc ggt ggt cat gat tac gag ggt att 528
Gly Leu Gln Ile Lys Ile Lys Ser Gly Gly His Asp Tyr Glu Gly Ile
165 170 175
tct tac aga tct gaa ttc cca ttc gtc atg ttg gac ttg tcc aat ttg 576
Ser Tyr Arg Ser Glu Phe Pro Phe Val Met Leu Asp Leu Ser Asn Leu
180 185 190
aac aag acc gaa atc gac ttg aac gaa gaa acc att tgg gtt caa gct 624
Asn Lys Thr Glu Ile Asp Leu Asn Glu Glu Thr Ile Trp Val Gln Ala
195 200 205
ggt gct act ttg ggt caa cta tat tac gct att gcc aac aga tcc aac 672
Gly Ala Thr Leu Gly Gln Leu Tyr Tyr Ala Ile Ala Asn Arg Ser Asn
210 215 220
gtt cat ggt ttt cca ggt ggt att tgt ttc tct gtt ggt act ggt ggt 720
Val His Gly Phe Pro Gly Gly Ile Cys Phe Ser Val Gly Thr Gly Gly
225 230 235 240
ttg att tct ggt ggt ggt tta ggt act atg atg aga aaa ttt ggt ttg 768
Leu Ile Ser Gly Gly Gly Leu Gly Thr Met Met Arg Lys Phe Gly Leu
245 250 255
gcc gct gat aac gtt gtt gat gct aga gtt atg gat gtc aac ggt aac 816
Ala Ala Asp Asn Val Val Asp Ala Arg Val Met Asp Val Asn Gly Asn
260 265 270
atc ttg gac cgt aaa aag atg aag aac gat ttg ttc tgg gca att aga 864
Ile Leu Asp Arg Lys Lys Met Lys Asn Asp Leu Phe Trp Ala Ile Arg
275 280 285
ggt ggt ggt ggc gct tct ttt ggt gtt att ttg gct tgg aaa ttg aag 912
Gly Gly Gly Gly Ala Ser Phe Gly Val Ile Leu Ala Trp Lys Leu Lys
290 295 300
ttg gtt agg gtt cca gaa aag gtt act gtt ttc acc gtc tgc aaa aag 960
Leu Val Arg Val Pro Glu Lys Val Thr Val Phe Thr Val Cys Lys Lys
305 310 315 320
ttc gat gat aac cag aac ttg atc ctg aag tgg gaa aac att gct cat 1008
Phe Asp Asp Asn Gln Asn Leu Ile Leu Lys Trp Glu Asn Ile Ala His
325 330 335
caa ttg cca gat aag ttg gtc atc aga gtt gtt atc caa aac gat ggt 1056
Gln Leu Pro Asp Lys Leu Val Ile Arg Val Val Ile Gln Asn Asp Gly
340 345 350
aca ggt aac gat aag tac gtc gaa atg aga ttt caa gcc ttg tat ttg 1104
Thr Gly Asn Asp Lys Tyr Val Glu Met Arg Phe Gln Ala Leu Tyr Leu
355 360 365
ggt cca gtt gat gaa ttg atc ccc ttg ttg aaa gaa aag ttc cca gaa 1152
Gly Pro Val Asp Glu Leu Ile Pro Leu Leu Lys Glu Lys Phe Pro Glu
370 375 380
ttc gac ttg gaa aag aag gat tgc ttg caa gaa cca gtt ggt gat aga 1200
Phe Asp Leu Glu Lys Lys Asp Cys Leu Gln Glu Pro Val Gly Asp Arg
385 390 395 400
tct aac aga tcc tgc att aac aaa gaa tgc aga gaa acc tct tgg atc 1248
Ser Asn Arg Ser Cys Ile Asn Lys Glu Cys Arg Glu Thr Ser Trp Ile
405 410 415
ggt tct gtc ttg tac ttt tac gaa aga gaa acc acc tcc tca ttg gag 1296
Gly Ser Val Leu Tyr Phe Tyr Glu Arg Glu Thr Thr Ser Ser Leu Glu
420 425 430
gtc tta ttg aaa gaa tcc att cca aag cag aag tcc tac tac aaa ggt 1344
Val Leu Leu Lys Glu Ser Ile Pro Lys Gln Lys Ser Tyr Tyr Lys Gly
435 440 445
act tct gat ttc gtc aag acc cag att tct gaa tct ggt tgg gaa atg 1392
Thr Ser Asp Phe Val Lys Thr Gln Ile Ser Glu Ser Gly Trp Glu Met
450 455 460
gtt gaa agg ttg ttg ttg gaa gaa gaa tcc cca aga att atc ttg gaa 1440
Val Glu Arg Leu Leu Leu Glu Glu Glu Ser Pro Arg Ile Ile Leu Glu
465 470 475 480
cca tta ggt ggt aag ttg gac gaa atc tcc gaa tca gaa att cca ttt 1488
Pro Leu Gly Gly Lys Leu Asp Glu Ile Ser Glu Ser Glu Ile Pro Phe
485 490 495
cca cac aga aaa ggc aac ttg tac aac atc caa tac ttg gtt tac tgg 1536
Pro His Arg Lys Gly Asn Leu Tyr Asn Ile Gln Tyr Leu Val Tyr Trp
500 505 510
cac gac aac aac gaa aac gtt tcc tct caa aaa att gcc tgg atg cgt 1584
His Asp Asn Asn Glu Asn Val Ser Ser Gln Lys Ile Ala Trp Met Arg
515 520 525
aag ctg tac aaa gaa atg gaa cca tac gtt gct aac tct cca aga act 1632
Lys Leu Tyr Lys Glu Met Glu Pro Tyr Val Ala Asn Ser Pro Arg Thr
530 535 540
gct tac ttg aac tac aga gat ttg gac ttc ggt tct aac gat gac aac 1680
Ala Tyr Leu Asn Tyr Arg Asp Leu Asp Phe Gly Ser Asn Asp Asp Asn
545 550 555 560
tac tct tac tcc aaa gct aaa atc tgg ggc gaa aag tac ttc aac tac 1728
Tyr Ser Tyr Ser Lys Ala Lys Ile Trp Gly Glu Lys Tyr Phe Asn Tyr
565 570 575
aac ttt gaa aga ctg gcc aag gtt aag tct aag gtt gat cca aac aac 1776
Asn Phe Glu Arg Leu Ala Lys Val Lys Ser Lys Val Asp Pro Asn Asn
580 585 590
ttc ttc aga acc gaa caa tct att ccc ttg tac caa acc tac tgg tca 1824
Phe Phe Arg Thr Glu Gln Ser Ile Pro Leu Tyr Gln Thr Tyr Trp Ser
595 600 605
cat cca caa ttt gaa aag 1842
His Pro Gln Phe Glu Lys
610
<210> 95
<211> 614
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 95
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser
1 5 10 15
Ala Leu Ala Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln
20 25 30
Ile Pro Ala Glu Ala Val Ile Gly Tyr Ser Asp Leu Glu Gly Asp Phe
35 40 45
Asp Val Ala Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu
50 55 60
Phe Ile Asn Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val
65 70 75 80
Ser Leu Glu Lys Arg Glu Ala Glu Ala Met Ser Arg Glu Asp Phe Leu
85 90 95
Arg Cys Leu Phe Lys Ser Ser Glu Ser Asn Val Thr Gln Asn Ile Tyr
100 105 110
Thr Pro Asn Ser Leu Thr Tyr Ser Ser Ile Leu Lys Tyr Ala Gln Lys
115 120 125
His Ser Arg Trp Val Asn Ser Ser Gln Pro Asn Phe Ile Ala Ser Pro
130 135 140
Thr Lys Glu Ser Glu Ile Lys Pro Val Ile Leu Cys Ala Lys Lys Leu
145 150 155 160
Gly Leu Gln Ile Lys Ile Lys Ser Gly Gly His Asp Tyr Glu Gly Ile
165 170 175
Ser Tyr Arg Ser Glu Phe Pro Phe Val Met Leu Asp Leu Ser Asn Leu
180 185 190
Asn Lys Thr Glu Ile Asp Leu Asn Glu Glu Thr Ile Trp Val Gln Ala
195 200 205
Gly Ala Thr Leu Gly Gln Leu Tyr Tyr Ala Ile Ala Asn Arg Ser Asn
210 215 220
Val His Gly Phe Pro Gly Gly Ile Cys Phe Ser Val Gly Thr Gly Gly
225 230 235 240
Leu Ile Ser Gly Gly Gly Leu Gly Thr Met Met Arg Lys Phe Gly Leu
245 250 255
Ala Ala Asp Asn Val Val Asp Ala Arg Val Met Asp Val Asn Gly Asn
260 265 270
Ile Leu Asp Arg Lys Lys Met Lys Asn Asp Leu Phe Trp Ala Ile Arg
275 280 285
Gly Gly Gly Gly Ala Ser Phe Gly Val Ile Leu Ala Trp Lys Leu Lys
290 295 300
Leu Val Arg Val Pro Glu Lys Val Thr Val Phe Thr Val Cys Lys Lys
305 310 315 320
Phe Asp Asp Asn Gln Asn Leu Ile Leu Lys Trp Glu Asn Ile Ala His
325 330 335
Gln Leu Pro Asp Lys Leu Val Ile Arg Val Val Ile Gln Asn Asp Gly
340 345 350
Thr Gly Asn Asp Lys Tyr Val Glu Met Arg Phe Gln Ala Leu Tyr Leu
355 360 365
Gly Pro Val Asp Glu Leu Ile Pro Leu Leu Lys Glu Lys Phe Pro Glu
370 375 380
Phe Asp Leu Glu Lys Lys Asp Cys Leu Gln Glu Pro Val Gly Asp Arg
385 390 395 400
Ser Asn Arg Ser Cys Ile Asn Lys Glu Cys Arg Glu Thr Ser Trp Ile
405 410 415
Gly Ser Val Leu Tyr Phe Tyr Glu Arg Glu Thr Thr Ser Ser Leu Glu
420 425 430
Val Leu Leu Lys Glu Ser Ile Pro Lys Gln Lys Ser Tyr Tyr Lys Gly
435 440 445
Thr Ser Asp Phe Val Lys Thr Gln Ile Ser Glu Ser Gly Trp Glu Met
450 455 460
Val Glu Arg Leu Leu Leu Glu Glu Glu Ser Pro Arg Ile Ile Leu Glu
465 470 475 480
Pro Leu Gly Gly Lys Leu Asp Glu Ile Ser Glu Ser Glu Ile Pro Phe
485 490 495
Pro His Arg Lys Gly Asn Leu Tyr Asn Ile Gln Tyr Leu Val Tyr Trp
500 505 510
His Asp Asn Asn Glu Asn Val Ser Ser Gln Lys Ile Ala Trp Met Arg
515 520 525
Lys Leu Tyr Lys Glu Met Glu Pro Tyr Val Ala Asn Ser Pro Arg Thr
530 535 540
Ala Tyr Leu Asn Tyr Arg Asp Leu Asp Phe Gly Ser Asn Asp Asp Asn
545 550 555 560
Tyr Ser Tyr Ser Lys Ala Lys Ile Trp Gly Glu Lys Tyr Phe Asn Tyr
565 570 575
Asn Phe Glu Arg Leu Ala Lys Val Lys Ser Lys Val Asp Pro Asn Asn
580 585 590
Phe Phe Arg Thr Glu Gln Ser Ile Pro Leu Tyr Gln Thr Tyr Trp Ser
595 600 605
His Pro Gln Phe Glu Lys
610
<210> 96
<211> 1641
<212> DNA
<213> 人工序列
<220>
<223> CBS133-A0A1U8MHW2
<220>
<221> CDS
<222> (1)..(1641)
<400> 96
atg ttc cca ttc ttg ttc gtc gtc ttg ttg tct ttg tct tgg aga atc 48
Met Phe Pro Phe Leu Phe Val Val Leu Leu Ser Leu Ser Trp Arg Ile
1 5 10 15
aat gct tct gct ttg cca gtt caa gaa ttc cta cat tgc ttg tct ttg 96
Asn Ala Ser Ala Leu Pro Val Gln Glu Phe Leu His Cys Leu Ser Leu
20 25 30
aga tcc cca aac tct tct att gcc aag ttg gtc tat acc cag aac aac 144
Arg Ser Pro Asn Ser Ser Ile Ala Lys Leu Val Tyr Thr Gln Asn Asn
35 40 45
tct tct tac tcc tct gtt ttg aaa tcc gct gct caa aac ttc aga ttc 192
Ser Ser Tyr Ser Ser Val Leu Lys Ser Ala Ala Gln Asn Phe Arg Phe
50 55 60
act act cca tct act cca act cca ttg gct ata gtt act cca ttg cat 240
Thr Thr Pro Ser Thr Pro Thr Pro Leu Ala Ile Val Thr Pro Leu His
65 70 75 80
gcc tct cat att caa gct gct gtt tac tgt tgt aga aaa cac ggt tta 288
Ala Ser His Ile Gln Ala Ala Val Tyr Cys Cys Arg Lys His Gly Leu
85 90 95
caa gtc aga act aga tct ggt ggt cat gat ttt gaa ggt ttg tct tac 336
Gln Val Arg Thr Arg Ser Gly Gly His Asp Phe Glu Gly Leu Ser Tyr
100 105 110
act tcc gct tac aag gtt cca ttc gtt gtt atc gat ttg gtc aac ttg 384
Thr Ser Ala Tyr Lys Val Pro Phe Val Val Ile Asp Leu Val Asn Leu
115 120 125
aga tcc gtt aga gtt aac gtt gaa aaa gct act gct tgg gtt gaa tct 432
Arg Ser Val Arg Val Asn Val Glu Lys Ala Thr Ala Trp Val Glu Ser
130 135 140
ggt gct act gtt ggt gaa tta tac gac gaa att gcc aga aag tct aag 480
Gly Ala Thr Val Gly Glu Leu Tyr Asp Glu Ile Ala Arg Lys Ser Lys
145 150 155 160
acc ttg ggt ttt cca gct ggt att tgt cca act tta ggt gtt ggt ggt 528
Thr Leu Gly Phe Pro Ala Gly Ile Cys Pro Thr Leu Gly Val Gly Gly
165 170 175
cac ttg tct ggt ggt ggt tat ggt ttg ttg ttt aga aag tat ggt ttg 576
His Leu Ser Gly Gly Gly Tyr Gly Leu Leu Phe Arg Lys Tyr Gly Leu
180 185 190
ggt gcc gat aac gtc att gat gct aga ttg att gat gtc aag ggt cgt 624
Gly Ala Asp Asn Val Ile Asp Ala Arg Leu Ile Asp Val Lys Gly Arg
195 200 205
atc ttg gac aaa aag tct atg ggt gaa gat ttg ttt tgg gcc att aga 672
Ile Leu Asp Lys Lys Ser Met Gly Glu Asp Leu Phe Trp Ala Ile Arg
210 215 220
ggt ggt ggt ggc ggt act ttt ggt ata gtt ttg gct tgg aaa ttg aag 720
Gly Gly Gly Gly Gly Thr Phe Gly Ile Val Leu Ala Trp Lys Leu Lys
225 230 235 240
ttg gtt gct gtt cca gct aac gtt act gtt ttc act gtt aga aag act 768
Leu Val Ala Val Pro Ala Asn Val Thr Val Phe Thr Val Arg Lys Thr
245 250 255
ttg gaa caa aac gcc act aag ttg gtt cat aga tgg caa tct att gcc 816
Leu Glu Gln Asn Ala Thr Lys Leu Val His Arg Trp Gln Ser Ile Ala
260 265 270
cac aag ttc cca aaa gaa atg cac ttg tct att gct att gct agg gct 864
His Lys Phe Pro Lys Glu Met His Leu Ser Ile Ala Ile Ala Arg Ala
275 280 285
aac tct tcc gaa gat gaa gaa aag atg act atc caa gct tct ttc acc 912
Asn Ser Ser Glu Asp Glu Glu Lys Met Thr Ile Gln Ala Ser Phe Thr
290 295 300
tct act ttc ttg ggt tcc att gat gaa ttg atc cca ttg atg gaa gaa 960
Ser Thr Phe Leu Gly Ser Ile Asp Glu Leu Ile Pro Leu Met Glu Glu
305 310 315 320
aag ttc cca gaa ttg ggt tta gtc aaa gaa gat tgc cta gaa atg tct 1008
Lys Phe Pro Glu Leu Gly Leu Val Lys Glu Asp Cys Leu Glu Met Ser
325 330 335
tgg gct gag tct aac ttg tac agc ttt ttg ttc ttg ctg aga tct cca 1056
Trp Ala Glu Ser Asn Leu Tyr Ser Phe Leu Phe Leu Leu Arg Ser Pro
340 345 350
tct gaa acc ctg ttg aat aga aac cag aag tcc tct atc tcc aaa acc 1104
Ser Glu Thr Leu Leu Asn Arg Asn Gln Lys Ser Ser Ile Ser Lys Thr
355 360 365
ttt ttc aag gcc aag tcc gat ttt gtc aaa cag cca att cca gaa tct 1152
Phe Phe Lys Ala Lys Ser Asp Phe Val Lys Gln Pro Ile Pro Glu Ser
370 375 380
gct ttc gaa ggt tta tgg tcc aag ttt tat gaa gat gag gct aag tcc 1200
Ala Phe Glu Gly Leu Trp Ser Lys Phe Tyr Glu Asp Glu Ala Lys Ser
385 390 395 400
gcc atc atg gtt ttt gtt gct ttt ggt ggt aag atg gac gaa atc ttg 1248
Ala Ile Met Val Phe Val Ala Phe Gly Gly Lys Met Asp Glu Ile Leu
405 410 415
gaa act gaa tac cca tat cca cat aga gcc ggt aac tta tac act atc 1296
Glu Thr Glu Tyr Pro Tyr Pro His Arg Ala Gly Asn Leu Tyr Thr Ile
420 425 430
ttg tac act gtt gac tgg gaa gag aaa gag aac atc aac tct gaa aag 1344
Leu Tyr Thr Val Asp Trp Glu Glu Lys Glu Asn Ile Asn Ser Glu Lys
435 440 445
ttc atg tcc tgg atg aga aga gtc tac aat tac atg act ccc tac gtt 1392
Phe Met Ser Trp Met Arg Arg Val Tyr Asn Tyr Met Thr Pro Tyr Val
450 455 460
tct aag tct cca aga gaa gct tac atc aac tac aga gat ttg gac atc 1440
Ser Lys Ser Pro Arg Glu Ala Tyr Ile Asn Tyr Arg Asp Leu Asp Ile
465 470 475 480
ggt aca aac aag atc aac aac aga aag aga tct tac gct aag gct aga 1488
Gly Thr Asn Lys Ile Asn Asn Arg Lys Arg Ser Tyr Ala Lys Ala Arg
485 490 495
gta tgg ggt gtt aag tac ttt aag aac aac ttc aac agg ctg gtt aac 1536
Val Trp Gly Val Lys Tyr Phe Lys Asn Asn Phe Asn Arg Leu Val Asn
500 505 510
gtc aag act ata gtt gac cca cag aat ttc ttc aga cac gaa caa tct 1584
Val Lys Thr Ile Val Asp Pro Gln Asn Phe Phe Arg His Glu Gln Ser
515 520 525
att cca cca cta ttc ttg tcc tgg aaa aga tcc gat cat aag aag cac 1632
Ile Pro Pro Leu Phe Leu Ser Trp Lys Arg Ser Asp His Lys Lys His
530 535 540
ttg tcc tcc 1641
Leu Ser Ser
545
<210> 97
<211> 547
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 97
Met Phe Pro Phe Leu Phe Val Val Leu Leu Ser Leu Ser Trp Arg Ile
1 5 10 15
Asn Ala Ser Ala Leu Pro Val Gln Glu Phe Leu His Cys Leu Ser Leu
20 25 30
Arg Ser Pro Asn Ser Ser Ile Ala Lys Leu Val Tyr Thr Gln Asn Asn
35 40 45
Ser Ser Tyr Ser Ser Val Leu Lys Ser Ala Ala Gln Asn Phe Arg Phe
50 55 60
Thr Thr Pro Ser Thr Pro Thr Pro Leu Ala Ile Val Thr Pro Leu His
65 70 75 80
Ala Ser His Ile Gln Ala Ala Val Tyr Cys Cys Arg Lys His Gly Leu
85 90 95
Gln Val Arg Thr Arg Ser Gly Gly His Asp Phe Glu Gly Leu Ser Tyr
100 105 110
Thr Ser Ala Tyr Lys Val Pro Phe Val Val Ile Asp Leu Val Asn Leu
115 120 125
Arg Ser Val Arg Val Asn Val Glu Lys Ala Thr Ala Trp Val Glu Ser
130 135 140
Gly Ala Thr Val Gly Glu Leu Tyr Asp Glu Ile Ala Arg Lys Ser Lys
145 150 155 160
Thr Leu Gly Phe Pro Ala Gly Ile Cys Pro Thr Leu Gly Val Gly Gly
165 170 175
His Leu Ser Gly Gly Gly Tyr Gly Leu Leu Phe Arg Lys Tyr Gly Leu
180 185 190
Gly Ala Asp Asn Val Ile Asp Ala Arg Leu Ile Asp Val Lys Gly Arg
195 200 205
Ile Leu Asp Lys Lys Ser Met Gly Glu Asp Leu Phe Trp Ala Ile Arg
210 215 220
Gly Gly Gly Gly Gly Thr Phe Gly Ile Val Leu Ala Trp Lys Leu Lys
225 230 235 240
Leu Val Ala Val Pro Ala Asn Val Thr Val Phe Thr Val Arg Lys Thr
245 250 255
Leu Glu Gln Asn Ala Thr Lys Leu Val His Arg Trp Gln Ser Ile Ala
260 265 270
His Lys Phe Pro Lys Glu Met His Leu Ser Ile Ala Ile Ala Arg Ala
275 280 285
Asn Ser Ser Glu Asp Glu Glu Lys Met Thr Ile Gln Ala Ser Phe Thr
290 295 300
Ser Thr Phe Leu Gly Ser Ile Asp Glu Leu Ile Pro Leu Met Glu Glu
305 310 315 320
Lys Phe Pro Glu Leu Gly Leu Val Lys Glu Asp Cys Leu Glu Met Ser
325 330 335
Trp Ala Glu Ser Asn Leu Tyr Ser Phe Leu Phe Leu Leu Arg Ser Pro
340 345 350
Ser Glu Thr Leu Leu Asn Arg Asn Gln Lys Ser Ser Ile Ser Lys Thr
355 360 365
Phe Phe Lys Ala Lys Ser Asp Phe Val Lys Gln Pro Ile Pro Glu Ser
370 375 380
Ala Phe Glu Gly Leu Trp Ser Lys Phe Tyr Glu Asp Glu Ala Lys Ser
385 390 395 400
Ala Ile Met Val Phe Val Ala Phe Gly Gly Lys Met Asp Glu Ile Leu
405 410 415
Glu Thr Glu Tyr Pro Tyr Pro His Arg Ala Gly Asn Leu Tyr Thr Ile
420 425 430
Leu Tyr Thr Val Asp Trp Glu Glu Lys Glu Asn Ile Asn Ser Glu Lys
435 440 445
Phe Met Ser Trp Met Arg Arg Val Tyr Asn Tyr Met Thr Pro Tyr Val
450 455 460
Ser Lys Ser Pro Arg Glu Ala Tyr Ile Asn Tyr Arg Asp Leu Asp Ile
465 470 475 480
Gly Thr Asn Lys Ile Asn Asn Arg Lys Arg Ser Tyr Ala Lys Ala Arg
485 490 495
Val Trp Gly Val Lys Tyr Phe Lys Asn Asn Phe Asn Arg Leu Val Asn
500 505 510
Val Lys Thr Ile Val Asp Pro Gln Asn Phe Phe Arg His Glu Gln Ser
515 520 525
Ile Pro Pro Leu Phe Leu Ser Trp Lys Arg Ser Asp His Lys Lys His
530 535 540
Leu Ser Ser
545
<210> 98
<211> 1587
<212> DNA
<213> 人工序列
<220>
<223> CBS133-A0A1U8MHW2-无信号肽
<220>
<221> CDS
<222> (1)..(1587)
<400> 98
atg gct ttg cca gtt caa gaa ttc cta cat tgc ttg tct ttg aga tcc 48
Met Ala Leu Pro Val Gln Glu Phe Leu His Cys Leu Ser Leu Arg Ser
1 5 10 15
cca aac tct tct att gcc aag ttg gtc tat acc cag aac aac tct tct 96
Pro Asn Ser Ser Ile Ala Lys Leu Val Tyr Thr Gln Asn Asn Ser Ser
20 25 30
tac tcc tct gtt ttg aaa tcc gct gct caa aac ttc aga ttc act act 144
Tyr Ser Ser Val Leu Lys Ser Ala Ala Gln Asn Phe Arg Phe Thr Thr
35 40 45
cca tct act cca act cca ttg gct ata gtt act cca ttg cat gcc tct 192
Pro Ser Thr Pro Thr Pro Leu Ala Ile Val Thr Pro Leu His Ala Ser
50 55 60
cat att caa gct gct gtt tac tgt tgt aga aaa cac ggt tta caa gtc 240
His Ile Gln Ala Ala Val Tyr Cys Cys Arg Lys His Gly Leu Gln Val
65 70 75 80
aga act aga tct ggt ggt cat gat ttt gaa ggt ttg tct tac act tcc 288
Arg Thr Arg Ser Gly Gly His Asp Phe Glu Gly Leu Ser Tyr Thr Ser
85 90 95
gct tac aag gtt cca ttc gtt gtt atc gat ttg gtc aac ttg aga tcc 336
Ala Tyr Lys Val Pro Phe Val Val Ile Asp Leu Val Asn Leu Arg Ser
100 105 110
gtt aga gtt aac gtt gaa aaa gct act gct tgg gtt gaa tct ggt gct 384
Val Arg Val Asn Val Glu Lys Ala Thr Ala Trp Val Glu Ser Gly Ala
115 120 125
act gtt ggt gaa tta tac gac gaa att gcc aga aag tct aag acc ttg 432
Thr Val Gly Glu Leu Tyr Asp Glu Ile Ala Arg Lys Ser Lys Thr Leu
130 135 140
ggt ttt cca gct ggt att tgt cca act tta ggt gtt ggt ggt cac ttg 480
Gly Phe Pro Ala Gly Ile Cys Pro Thr Leu Gly Val Gly Gly His Leu
145 150 155 160
tct ggt ggt ggt tat ggt ttg ttg ttt aga aag tat ggt ttg ggt gcc 528
Ser Gly Gly Gly Tyr Gly Leu Leu Phe Arg Lys Tyr Gly Leu Gly Ala
165 170 175
gat aac gtc att gat gct aga ttg att gat gtc aag ggt cgt atc ttg 576
Asp Asn Val Ile Asp Ala Arg Leu Ile Asp Val Lys Gly Arg Ile Leu
180 185 190
gac aaa aag tct atg ggt gaa gat ttg ttt tgg gcc att aga ggt ggt 624
Asp Lys Lys Ser Met Gly Glu Asp Leu Phe Trp Ala Ile Arg Gly Gly
195 200 205
ggt ggc ggt act ttt ggt ata gtt ttg gct tgg aaa ttg aag ttg gtt 672
Gly Gly Gly Thr Phe Gly Ile Val Leu Ala Trp Lys Leu Lys Leu Val
210 215 220
gct gtt cca gct aac gtt act gtt ttc act gtt aga aag act ttg gaa 720
Ala Val Pro Ala Asn Val Thr Val Phe Thr Val Arg Lys Thr Leu Glu
225 230 235 240
caa aac gcc act aag ttg gtt cat aga tgg caa tct att gcc cac aag 768
Gln Asn Ala Thr Lys Leu Val His Arg Trp Gln Ser Ile Ala His Lys
245 250 255
ttc cca aaa gaa atg cac ttg tct att gct att gct agg gct aac tct 816
Phe Pro Lys Glu Met His Leu Ser Ile Ala Ile Ala Arg Ala Asn Ser
260 265 270
tcc gaa gat gaa gaa aag atg act atc caa gct tct ttc acc tct act 864
Ser Glu Asp Glu Glu Lys Met Thr Ile Gln Ala Ser Phe Thr Ser Thr
275 280 285
ttc ttg ggt tcc att gat gaa ttg atc cca ttg atg gaa gaa aag ttc 912
Phe Leu Gly Ser Ile Asp Glu Leu Ile Pro Leu Met Glu Glu Lys Phe
290 295 300
cca gaa ttg ggt tta gtc aaa gaa gat tgc cta gaa atg tct tgg gct 960
Pro Glu Leu Gly Leu Val Lys Glu Asp Cys Leu Glu Met Ser Trp Ala
305 310 315 320
gag tct aac ttg tac agc ttt ttg ttc ttg ctg aga tct cca tct gaa 1008
Glu Ser Asn Leu Tyr Ser Phe Leu Phe Leu Leu Arg Ser Pro Ser Glu
325 330 335
acc ctg ttg aat aga aac cag aag tcc tct atc tcc aaa acc ttt ttc 1056
Thr Leu Leu Asn Arg Asn Gln Lys Ser Ser Ile Ser Lys Thr Phe Phe
340 345 350
aag gcc aag tcc gat ttt gtc aaa cag cca att cca gaa tct gct ttc 1104
Lys Ala Lys Ser Asp Phe Val Lys Gln Pro Ile Pro Glu Ser Ala Phe
355 360 365
gaa ggt tta tgg tcc aag ttt tat gaa gat gag gct aag tcc gcc atc 1152
Glu Gly Leu Trp Ser Lys Phe Tyr Glu Asp Glu Ala Lys Ser Ala Ile
370 375 380
atg gtt ttt gtt gct ttt ggt ggt aag atg gac gaa atc ttg gaa act 1200
Met Val Phe Val Ala Phe Gly Gly Lys Met Asp Glu Ile Leu Glu Thr
385 390 395 400
gaa tac cca tat cca cat aga gcc ggt aac tta tac act atc ttg tac 1248
Glu Tyr Pro Tyr Pro His Arg Ala Gly Asn Leu Tyr Thr Ile Leu Tyr
405 410 415
act gtt gac tgg gaa gag aaa gag aac atc aac tct gaa aag ttc atg 1296
Thr Val Asp Trp Glu Glu Lys Glu Asn Ile Asn Ser Glu Lys Phe Met
420 425 430
tcc tgg atg aga aga gtc tac aat tac atg act ccc tac gtt tct aag 1344
Ser Trp Met Arg Arg Val Tyr Asn Tyr Met Thr Pro Tyr Val Ser Lys
435 440 445
tct cca aga gaa gct tac atc aac tac aga gat ttg gac atc ggt aca 1392
Ser Pro Arg Glu Ala Tyr Ile Asn Tyr Arg Asp Leu Asp Ile Gly Thr
450 455 460
aac aag atc aac aac aga aag aga tct tac gct aag gct aga gta tgg 1440
Asn Lys Ile Asn Asn Arg Lys Arg Ser Tyr Ala Lys Ala Arg Val Trp
465 470 475 480
ggt gtt aag tac ttt aag aac aac ttc aac agg ctg gtt aac gtc aag 1488
Gly Val Lys Tyr Phe Lys Asn Asn Phe Asn Arg Leu Val Asn Val Lys
485 490 495
act ata gtt gac cca cag aat ttc ttc aga cac gaa caa tct att cca 1536
Thr Ile Val Asp Pro Gln Asn Phe Phe Arg His Glu Gln Ser Ile Pro
500 505 510
cca cta ttc ttg tcc tgg aaa aga tcc gat cat aag aag cac ttg tcc 1584
Pro Leu Phe Leu Ser Trp Lys Arg Ser Asp His Lys Lys His Leu Ser
515 520 525
tcc 1587
Ser
<210> 99
<211> 529
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 99
Met Ala Leu Pro Val Gln Glu Phe Leu His Cys Leu Ser Leu Arg Ser
1 5 10 15
Pro Asn Ser Ser Ile Ala Lys Leu Val Tyr Thr Gln Asn Asn Ser Ser
20 25 30
Tyr Ser Ser Val Leu Lys Ser Ala Ala Gln Asn Phe Arg Phe Thr Thr
35 40 45
Pro Ser Thr Pro Thr Pro Leu Ala Ile Val Thr Pro Leu His Ala Ser
50 55 60
His Ile Gln Ala Ala Val Tyr Cys Cys Arg Lys His Gly Leu Gln Val
65 70 75 80
Arg Thr Arg Ser Gly Gly His Asp Phe Glu Gly Leu Ser Tyr Thr Ser
85 90 95
Ala Tyr Lys Val Pro Phe Val Val Ile Asp Leu Val Asn Leu Arg Ser
100 105 110
Val Arg Val Asn Val Glu Lys Ala Thr Ala Trp Val Glu Ser Gly Ala
115 120 125
Thr Val Gly Glu Leu Tyr Asp Glu Ile Ala Arg Lys Ser Lys Thr Leu
130 135 140
Gly Phe Pro Ala Gly Ile Cys Pro Thr Leu Gly Val Gly Gly His Leu
145 150 155 160
Ser Gly Gly Gly Tyr Gly Leu Leu Phe Arg Lys Tyr Gly Leu Gly Ala
165 170 175
Asp Asn Val Ile Asp Ala Arg Leu Ile Asp Val Lys Gly Arg Ile Leu
180 185 190
Asp Lys Lys Ser Met Gly Glu Asp Leu Phe Trp Ala Ile Arg Gly Gly
195 200 205
Gly Gly Gly Thr Phe Gly Ile Val Leu Ala Trp Lys Leu Lys Leu Val
210 215 220
Ala Val Pro Ala Asn Val Thr Val Phe Thr Val Arg Lys Thr Leu Glu
225 230 235 240
Gln Asn Ala Thr Lys Leu Val His Arg Trp Gln Ser Ile Ala His Lys
245 250 255
Phe Pro Lys Glu Met His Leu Ser Ile Ala Ile Ala Arg Ala Asn Ser
260 265 270
Ser Glu Asp Glu Glu Lys Met Thr Ile Gln Ala Ser Phe Thr Ser Thr
275 280 285
Phe Leu Gly Ser Ile Asp Glu Leu Ile Pro Leu Met Glu Glu Lys Phe
290 295 300
Pro Glu Leu Gly Leu Val Lys Glu Asp Cys Leu Glu Met Ser Trp Ala
305 310 315 320
Glu Ser Asn Leu Tyr Ser Phe Leu Phe Leu Leu Arg Ser Pro Ser Glu
325 330 335
Thr Leu Leu Asn Arg Asn Gln Lys Ser Ser Ile Ser Lys Thr Phe Phe
340 345 350
Lys Ala Lys Ser Asp Phe Val Lys Gln Pro Ile Pro Glu Ser Ala Phe
355 360 365
Glu Gly Leu Trp Ser Lys Phe Tyr Glu Asp Glu Ala Lys Ser Ala Ile
370 375 380
Met Val Phe Val Ala Phe Gly Gly Lys Met Asp Glu Ile Leu Glu Thr
385 390 395 400
Glu Tyr Pro Tyr Pro His Arg Ala Gly Asn Leu Tyr Thr Ile Leu Tyr
405 410 415
Thr Val Asp Trp Glu Glu Lys Glu Asn Ile Asn Ser Glu Lys Phe Met
420 425 430
Ser Trp Met Arg Arg Val Tyr Asn Tyr Met Thr Pro Tyr Val Ser Lys
435 440 445
Ser Pro Arg Glu Ala Tyr Ile Asn Tyr Arg Asp Leu Asp Ile Gly Thr
450 455 460
Asn Lys Ile Asn Asn Arg Lys Arg Ser Tyr Ala Lys Ala Arg Val Trp
465 470 475 480
Gly Val Lys Tyr Phe Lys Asn Asn Phe Asn Arg Leu Val Asn Val Lys
485 490 495
Thr Ile Val Asp Pro Gln Asn Phe Phe Arg His Glu Gln Ser Ile Pro
500 505 510
Pro Leu Phe Leu Ser Trp Lys Arg Ser Asp His Lys Lys His Leu Ser
515 520 525
Ser
<210> 100
<211> 1611
<212> DNA
<213> 人工序列
<220>
<223> CBS133-A0A1U8MHW2-无信号肽-strep标签
<220>
<221> CDS
<222> (1)..(1611)
<400> 100
atg gct ttg cca gtt caa gaa ttc cta cat tgc ttg tct ttg aga tcc 48
Met Ala Leu Pro Val Gln Glu Phe Leu His Cys Leu Ser Leu Arg Ser
1 5 10 15
cca aac tct tct att gcc aag ttg gtc tat acc cag aac aac tct tct 96
Pro Asn Ser Ser Ile Ala Lys Leu Val Tyr Thr Gln Asn Asn Ser Ser
20 25 30
tac tcc tct gtt ttg aaa tcc gct gct caa aac ttc aga ttc act act 144
Tyr Ser Ser Val Leu Lys Ser Ala Ala Gln Asn Phe Arg Phe Thr Thr
35 40 45
cca tct act cca act cca ttg gct ata gtt act cca ttg cat gcc tct 192
Pro Ser Thr Pro Thr Pro Leu Ala Ile Val Thr Pro Leu His Ala Ser
50 55 60
cat att caa gct gct gtt tac tgt tgt aga aaa cac ggt tta caa gtc 240
His Ile Gln Ala Ala Val Tyr Cys Cys Arg Lys His Gly Leu Gln Val
65 70 75 80
aga act aga tct ggt ggt cat gat ttt gaa ggt ttg tct tac act tcc 288
Arg Thr Arg Ser Gly Gly His Asp Phe Glu Gly Leu Ser Tyr Thr Ser
85 90 95
gct tac aag gtt cca ttc gtt gtt atc gat ttg gtc aac ttg aga tcc 336
Ala Tyr Lys Val Pro Phe Val Val Ile Asp Leu Val Asn Leu Arg Ser
100 105 110
gtt aga gtt aac gtt gaa aaa gct act gct tgg gtt gaa tct ggt gct 384
Val Arg Val Asn Val Glu Lys Ala Thr Ala Trp Val Glu Ser Gly Ala
115 120 125
act gtt ggt gaa tta tac gac gaa att gcc aga aag tct aag acc ttg 432
Thr Val Gly Glu Leu Tyr Asp Glu Ile Ala Arg Lys Ser Lys Thr Leu
130 135 140
ggt ttt cca gct ggt att tgt cca act tta ggt gtt ggt ggt cac ttg 480
Gly Phe Pro Ala Gly Ile Cys Pro Thr Leu Gly Val Gly Gly His Leu
145 150 155 160
tct ggt ggt ggt tat ggt ttg ttg ttt aga aag tat ggt ttg ggt gcc 528
Ser Gly Gly Gly Tyr Gly Leu Leu Phe Arg Lys Tyr Gly Leu Gly Ala
165 170 175
gat aac gtc att gat gct aga ttg att gat gtc aag ggt cgt atc ttg 576
Asp Asn Val Ile Asp Ala Arg Leu Ile Asp Val Lys Gly Arg Ile Leu
180 185 190
gac aaa aag tct atg ggt gaa gat ttg ttt tgg gcc att aga ggt ggt 624
Asp Lys Lys Ser Met Gly Glu Asp Leu Phe Trp Ala Ile Arg Gly Gly
195 200 205
ggt ggc ggt act ttt ggt ata gtt ttg gct tgg aaa ttg aag ttg gtt 672
Gly Gly Gly Thr Phe Gly Ile Val Leu Ala Trp Lys Leu Lys Leu Val
210 215 220
gct gtt cca gct aac gtt act gtt ttc act gtt aga aag act ttg gaa 720
Ala Val Pro Ala Asn Val Thr Val Phe Thr Val Arg Lys Thr Leu Glu
225 230 235 240
caa aac gcc act aag ttg gtt cat aga tgg caa tct att gcc cac aag 768
Gln Asn Ala Thr Lys Leu Val His Arg Trp Gln Ser Ile Ala His Lys
245 250 255
ttc cca aaa gaa atg cac ttg tct att gct att gct agg gct aac tct 816
Phe Pro Lys Glu Met His Leu Ser Ile Ala Ile Ala Arg Ala Asn Ser
260 265 270
tcc gaa gat gaa gaa aag atg act atc caa gct tct ttc acc tct act 864
Ser Glu Asp Glu Glu Lys Met Thr Ile Gln Ala Ser Phe Thr Ser Thr
275 280 285
ttc ttg ggt tcc att gat gaa ttg atc cca ttg atg gaa gaa aag ttc 912
Phe Leu Gly Ser Ile Asp Glu Leu Ile Pro Leu Met Glu Glu Lys Phe
290 295 300
cca gaa ttg ggt tta gtc aaa gaa gat tgc cta gaa atg tct tgg gct 960
Pro Glu Leu Gly Leu Val Lys Glu Asp Cys Leu Glu Met Ser Trp Ala
305 310 315 320
gag tct aac ttg tac agc ttt ttg ttc ttg ctg aga tct cca tct gaa 1008
Glu Ser Asn Leu Tyr Ser Phe Leu Phe Leu Leu Arg Ser Pro Ser Glu
325 330 335
acc ctg ttg aat aga aac cag aag tcc tct atc tcc aaa acc ttt ttc 1056
Thr Leu Leu Asn Arg Asn Gln Lys Ser Ser Ile Ser Lys Thr Phe Phe
340 345 350
aag gcc aag tcc gat ttt gtc aaa cag cca att cca gaa tct gct ttc 1104
Lys Ala Lys Ser Asp Phe Val Lys Gln Pro Ile Pro Glu Ser Ala Phe
355 360 365
gaa ggt tta tgg tcc aag ttt tat gaa gat gag gct aag tcc gcc atc 1152
Glu Gly Leu Trp Ser Lys Phe Tyr Glu Asp Glu Ala Lys Ser Ala Ile
370 375 380
atg gtt ttt gtt gct ttt ggt ggt aag atg gac gaa atc ttg gaa act 1200
Met Val Phe Val Ala Phe Gly Gly Lys Met Asp Glu Ile Leu Glu Thr
385 390 395 400
gaa tac cca tat cca cat aga gcc ggt aac tta tac act atc ttg tac 1248
Glu Tyr Pro Tyr Pro His Arg Ala Gly Asn Leu Tyr Thr Ile Leu Tyr
405 410 415
act gtt gac tgg gaa gag aaa gag aac atc aac tct gaa aag ttc atg 1296
Thr Val Asp Trp Glu Glu Lys Glu Asn Ile Asn Ser Glu Lys Phe Met
420 425 430
tcc tgg atg aga aga gtc tac aat tac atg act ccc tac gtt tct aag 1344
Ser Trp Met Arg Arg Val Tyr Asn Tyr Met Thr Pro Tyr Val Ser Lys
435 440 445
tct cca aga gaa gct tac atc aac tac aga gat ttg gac atc ggt aca 1392
Ser Pro Arg Glu Ala Tyr Ile Asn Tyr Arg Asp Leu Asp Ile Gly Thr
450 455 460
aac aag atc aac aac aga aag aga tct tac gct aag gct aga gta tgg 1440
Asn Lys Ile Asn Asn Arg Lys Arg Ser Tyr Ala Lys Ala Arg Val Trp
465 470 475 480
ggt gtt aag tac ttt aag aac aac ttc aac agg ctg gtt aac gtc aag 1488
Gly Val Lys Tyr Phe Lys Asn Asn Phe Asn Arg Leu Val Asn Val Lys
485 490 495
act ata gtt gac cca cag aat ttc ttc aga cac gaa caa tct att cca 1536
Thr Ile Val Asp Pro Gln Asn Phe Phe Arg His Glu Gln Ser Ile Pro
500 505 510
cca cta ttc ttg tcc tgg aaa aga tcc gat cat aag aag cac ttg tcc 1584
Pro Leu Phe Leu Ser Trp Lys Arg Ser Asp His Lys Lys His Leu Ser
515 520 525
tcc tgg tca cat cca caa ttt gaa aag 1611
Ser Trp Ser His Pro Gln Phe Glu Lys
530 535
<210> 101
<211> 537
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 101
Met Ala Leu Pro Val Gln Glu Phe Leu His Cys Leu Ser Leu Arg Ser
1 5 10 15
Pro Asn Ser Ser Ile Ala Lys Leu Val Tyr Thr Gln Asn Asn Ser Ser
20 25 30
Tyr Ser Ser Val Leu Lys Ser Ala Ala Gln Asn Phe Arg Phe Thr Thr
35 40 45
Pro Ser Thr Pro Thr Pro Leu Ala Ile Val Thr Pro Leu His Ala Ser
50 55 60
His Ile Gln Ala Ala Val Tyr Cys Cys Arg Lys His Gly Leu Gln Val
65 70 75 80
Arg Thr Arg Ser Gly Gly His Asp Phe Glu Gly Leu Ser Tyr Thr Ser
85 90 95
Ala Tyr Lys Val Pro Phe Val Val Ile Asp Leu Val Asn Leu Arg Ser
100 105 110
Val Arg Val Asn Val Glu Lys Ala Thr Ala Trp Val Glu Ser Gly Ala
115 120 125
Thr Val Gly Glu Leu Tyr Asp Glu Ile Ala Arg Lys Ser Lys Thr Leu
130 135 140
Gly Phe Pro Ala Gly Ile Cys Pro Thr Leu Gly Val Gly Gly His Leu
145 150 155 160
Ser Gly Gly Gly Tyr Gly Leu Leu Phe Arg Lys Tyr Gly Leu Gly Ala
165 170 175
Asp Asn Val Ile Asp Ala Arg Leu Ile Asp Val Lys Gly Arg Ile Leu
180 185 190
Asp Lys Lys Ser Met Gly Glu Asp Leu Phe Trp Ala Ile Arg Gly Gly
195 200 205
Gly Gly Gly Thr Phe Gly Ile Val Leu Ala Trp Lys Leu Lys Leu Val
210 215 220
Ala Val Pro Ala Asn Val Thr Val Phe Thr Val Arg Lys Thr Leu Glu
225 230 235 240
Gln Asn Ala Thr Lys Leu Val His Arg Trp Gln Ser Ile Ala His Lys
245 250 255
Phe Pro Lys Glu Met His Leu Ser Ile Ala Ile Ala Arg Ala Asn Ser
260 265 270
Ser Glu Asp Glu Glu Lys Met Thr Ile Gln Ala Ser Phe Thr Ser Thr
275 280 285
Phe Leu Gly Ser Ile Asp Glu Leu Ile Pro Leu Met Glu Glu Lys Phe
290 295 300
Pro Glu Leu Gly Leu Val Lys Glu Asp Cys Leu Glu Met Ser Trp Ala
305 310 315 320
Glu Ser Asn Leu Tyr Ser Phe Leu Phe Leu Leu Arg Ser Pro Ser Glu
325 330 335
Thr Leu Leu Asn Arg Asn Gln Lys Ser Ser Ile Ser Lys Thr Phe Phe
340 345 350
Lys Ala Lys Ser Asp Phe Val Lys Gln Pro Ile Pro Glu Ser Ala Phe
355 360 365
Glu Gly Leu Trp Ser Lys Phe Tyr Glu Asp Glu Ala Lys Ser Ala Ile
370 375 380
Met Val Phe Val Ala Phe Gly Gly Lys Met Asp Glu Ile Leu Glu Thr
385 390 395 400
Glu Tyr Pro Tyr Pro His Arg Ala Gly Asn Leu Tyr Thr Ile Leu Tyr
405 410 415
Thr Val Asp Trp Glu Glu Lys Glu Asn Ile Asn Ser Glu Lys Phe Met
420 425 430
Ser Trp Met Arg Arg Val Tyr Asn Tyr Met Thr Pro Tyr Val Ser Lys
435 440 445
Ser Pro Arg Glu Ala Tyr Ile Asn Tyr Arg Asp Leu Asp Ile Gly Thr
450 455 460
Asn Lys Ile Asn Asn Arg Lys Arg Ser Tyr Ala Lys Ala Arg Val Trp
465 470 475 480
Gly Val Lys Tyr Phe Lys Asn Asn Phe Asn Arg Leu Val Asn Val Lys
485 490 495
Thr Ile Val Asp Pro Gln Asn Phe Phe Arg His Glu Gln Ser Ile Pro
500 505 510
Pro Leu Phe Leu Ser Trp Lys Arg Ser Asp His Lys Lys His Leu Ser
515 520 525
Ser Trp Ser His Pro Gln Phe Glu Lys
530 535
<210> 102
<211> 1668
<212> DNA
<213> 人工序列
<220>
<223> CBS133-A0A1U8MHW2-截断alpha因子-无信号肽-strep标签
<220>
<221> CDS
<222> (1)..(1668)
<400> 102
atg aga ttt cct tcc atc ttc acg gct gtg cta ttt gca gca tcc tcc 48
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser
1 5 10 15
gca ctt gca atg gct ttg cca gtt caa gaa ttc cta cat tgc ttg tct 96
Ala Leu Ala Met Ala Leu Pro Val Gln Glu Phe Leu His Cys Leu Ser
20 25 30
ttg aga tcc cca aac tct tct att gcc aag ttg gtc tat acc cag aac 144
Leu Arg Ser Pro Asn Ser Ser Ile Ala Lys Leu Val Tyr Thr Gln Asn
35 40 45
aac tct tct tac tcc tct gtt ttg aaa tcc gct gct caa aac ttc aga 192
Asn Ser Ser Tyr Ser Ser Val Leu Lys Ser Ala Ala Gln Asn Phe Arg
50 55 60
ttc act act cca tct act cca act cca ttg gct ata gtt act cca ttg 240
Phe Thr Thr Pro Ser Thr Pro Thr Pro Leu Ala Ile Val Thr Pro Leu
65 70 75 80
cat gcc tct cat att caa gct gct gtt tac tgt tgt aga aaa cac ggt 288
His Ala Ser His Ile Gln Ala Ala Val Tyr Cys Cys Arg Lys His Gly
85 90 95
tta caa gtc aga act aga tct ggt ggt cat gat ttt gaa ggt ttg tct 336
Leu Gln Val Arg Thr Arg Ser Gly Gly His Asp Phe Glu Gly Leu Ser
100 105 110
tac act tcc gct tac aag gtt cca ttc gtt gtt atc gat ttg gtc aac 384
Tyr Thr Ser Ala Tyr Lys Val Pro Phe Val Val Ile Asp Leu Val Asn
115 120 125
ttg aga tcc gtt aga gtt aac gtt gaa aaa gct act gct tgg gtt gaa 432
Leu Arg Ser Val Arg Val Asn Val Glu Lys Ala Thr Ala Trp Val Glu
130 135 140
tct ggt gct act gtt ggt gaa tta tac gac gaa att gcc aga aag tct 480
Ser Gly Ala Thr Val Gly Glu Leu Tyr Asp Glu Ile Ala Arg Lys Ser
145 150 155 160
aag acc ttg ggt ttt cca gct ggt att tgt cca act tta ggt gtt ggt 528
Lys Thr Leu Gly Phe Pro Ala Gly Ile Cys Pro Thr Leu Gly Val Gly
165 170 175
ggt cac ttg tct ggt ggt ggt tat ggt ttg ttg ttt aga aag tat ggt 576
Gly His Leu Ser Gly Gly Gly Tyr Gly Leu Leu Phe Arg Lys Tyr Gly
180 185 190
ttg ggt gcc gat aac gtc att gat gct aga ttg att gat gtc aag ggt 624
Leu Gly Ala Asp Asn Val Ile Asp Ala Arg Leu Ile Asp Val Lys Gly
195 200 205
cgt atc ttg gac aaa aag tct atg ggt gaa gat ttg ttt tgg gcc att 672
Arg Ile Leu Asp Lys Lys Ser Met Gly Glu Asp Leu Phe Trp Ala Ile
210 215 220
aga ggt ggt ggt ggc ggt act ttt ggt ata gtt ttg gct tgg aaa ttg 720
Arg Gly Gly Gly Gly Gly Thr Phe Gly Ile Val Leu Ala Trp Lys Leu
225 230 235 240
aag ttg gtt gct gtt cca gct aac gtt act gtt ttc act gtt aga aag 768
Lys Leu Val Ala Val Pro Ala Asn Val Thr Val Phe Thr Val Arg Lys
245 250 255
act ttg gaa caa aac gcc act aag ttg gtt cat aga tgg caa tct att 816
Thr Leu Glu Gln Asn Ala Thr Lys Leu Val His Arg Trp Gln Ser Ile
260 265 270
gcc cac aag ttc cca aaa gaa atg cac ttg tct att gct att gct agg 864
Ala His Lys Phe Pro Lys Glu Met His Leu Ser Ile Ala Ile Ala Arg
275 280 285
gct aac tct tcc gaa gat gaa gaa aag atg act atc caa gct tct ttc 912
Ala Asn Ser Ser Glu Asp Glu Glu Lys Met Thr Ile Gln Ala Ser Phe
290 295 300
acc tct act ttc ttg ggt tcc att gat gaa ttg atc cca ttg atg gaa 960
Thr Ser Thr Phe Leu Gly Ser Ile Asp Glu Leu Ile Pro Leu Met Glu
305 310 315 320
gaa aag ttc cca gaa ttg ggt tta gtc aaa gaa gat tgc cta gaa atg 1008
Glu Lys Phe Pro Glu Leu Gly Leu Val Lys Glu Asp Cys Leu Glu Met
325 330 335
tct tgg gct gag tct aac ttg tac agc ttt ttg ttc ttg ctg aga tct 1056
Ser Trp Ala Glu Ser Asn Leu Tyr Ser Phe Leu Phe Leu Leu Arg Ser
340 345 350
cca tct gaa acc ctg ttg aat aga aac cag aag tcc tct atc tcc aaa 1104
Pro Ser Glu Thr Leu Leu Asn Arg Asn Gln Lys Ser Ser Ile Ser Lys
355 360 365
acc ttt ttc aag gcc aag tcc gat ttt gtc aaa cag cca att cca gaa 1152
Thr Phe Phe Lys Ala Lys Ser Asp Phe Val Lys Gln Pro Ile Pro Glu
370 375 380
tct gct ttc gaa ggt tta tgg tcc aag ttt tat gaa gat gag gct aag 1200
Ser Ala Phe Glu Gly Leu Trp Ser Lys Phe Tyr Glu Asp Glu Ala Lys
385 390 395 400
tcc gcc atc atg gtt ttt gtt gct ttt ggt ggt aag atg gac gaa atc 1248
Ser Ala Ile Met Val Phe Val Ala Phe Gly Gly Lys Met Asp Glu Ile
405 410 415
ttg gaa act gaa tac cca tat cca cat aga gcc ggt aac tta tac act 1296
Leu Glu Thr Glu Tyr Pro Tyr Pro His Arg Ala Gly Asn Leu Tyr Thr
420 425 430
atc ttg tac act gtt gac tgg gaa gag aaa gag aac atc aac tct gaa 1344
Ile Leu Tyr Thr Val Asp Trp Glu Glu Lys Glu Asn Ile Asn Ser Glu
435 440 445
aag ttc atg tcc tgg atg aga aga gtc tac aat tac atg act ccc tac 1392
Lys Phe Met Ser Trp Met Arg Arg Val Tyr Asn Tyr Met Thr Pro Tyr
450 455 460
gtt tct aag tct cca aga gaa gct tac atc aac tac aga gat ttg gac 1440
Val Ser Lys Ser Pro Arg Glu Ala Tyr Ile Asn Tyr Arg Asp Leu Asp
465 470 475 480
atc ggt aca aac aag atc aac aac aga aag aga tct tac gct aag gct 1488
Ile Gly Thr Asn Lys Ile Asn Asn Arg Lys Arg Ser Tyr Ala Lys Ala
485 490 495
aga gta tgg ggt gtt aag tac ttt aag aac aac ttc aac agg ctg gtt 1536
Arg Val Trp Gly Val Lys Tyr Phe Lys Asn Asn Phe Asn Arg Leu Val
500 505 510
aac gtc aag act ata gtt gac cca cag aat ttc ttc aga cac gaa caa 1584
Asn Val Lys Thr Ile Val Asp Pro Gln Asn Phe Phe Arg His Glu Gln
515 520 525
tct att cca cca cta ttc ttg tcc tgg aaa aga tcc gat cat aag aag 1632
Ser Ile Pro Pro Leu Phe Leu Ser Trp Lys Arg Ser Asp His Lys Lys
530 535 540
cac ttg tcc tcc tgg tca cat cca caa ttt gaa aag 1668
His Leu Ser Ser Trp Ser His Pro Gln Phe Glu Lys
545 550 555
<210> 103
<211> 556
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 103
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser
1 5 10 15
Ala Leu Ala Met Ala Leu Pro Val Gln Glu Phe Leu His Cys Leu Ser
20 25 30
Leu Arg Ser Pro Asn Ser Ser Ile Ala Lys Leu Val Tyr Thr Gln Asn
35 40 45
Asn Ser Ser Tyr Ser Ser Val Leu Lys Ser Ala Ala Gln Asn Phe Arg
50 55 60
Phe Thr Thr Pro Ser Thr Pro Thr Pro Leu Ala Ile Val Thr Pro Leu
65 70 75 80
His Ala Ser His Ile Gln Ala Ala Val Tyr Cys Cys Arg Lys His Gly
85 90 95
Leu Gln Val Arg Thr Arg Ser Gly Gly His Asp Phe Glu Gly Leu Ser
100 105 110
Tyr Thr Ser Ala Tyr Lys Val Pro Phe Val Val Ile Asp Leu Val Asn
115 120 125
Leu Arg Ser Val Arg Val Asn Val Glu Lys Ala Thr Ala Trp Val Glu
130 135 140
Ser Gly Ala Thr Val Gly Glu Leu Tyr Asp Glu Ile Ala Arg Lys Ser
145 150 155 160
Lys Thr Leu Gly Phe Pro Ala Gly Ile Cys Pro Thr Leu Gly Val Gly
165 170 175
Gly His Leu Ser Gly Gly Gly Tyr Gly Leu Leu Phe Arg Lys Tyr Gly
180 185 190
Leu Gly Ala Asp Asn Val Ile Asp Ala Arg Leu Ile Asp Val Lys Gly
195 200 205
Arg Ile Leu Asp Lys Lys Ser Met Gly Glu Asp Leu Phe Trp Ala Ile
210 215 220
Arg Gly Gly Gly Gly Gly Thr Phe Gly Ile Val Leu Ala Trp Lys Leu
225 230 235 240
Lys Leu Val Ala Val Pro Ala Asn Val Thr Val Phe Thr Val Arg Lys
245 250 255
Thr Leu Glu Gln Asn Ala Thr Lys Leu Val His Arg Trp Gln Ser Ile
260 265 270
Ala His Lys Phe Pro Lys Glu Met His Leu Ser Ile Ala Ile Ala Arg
275 280 285
Ala Asn Ser Ser Glu Asp Glu Glu Lys Met Thr Ile Gln Ala Ser Phe
290 295 300
Thr Ser Thr Phe Leu Gly Ser Ile Asp Glu Leu Ile Pro Leu Met Glu
305 310 315 320
Glu Lys Phe Pro Glu Leu Gly Leu Val Lys Glu Asp Cys Leu Glu Met
325 330 335
Ser Trp Ala Glu Ser Asn Leu Tyr Ser Phe Leu Phe Leu Leu Arg Ser
340 345 350
Pro Ser Glu Thr Leu Leu Asn Arg Asn Gln Lys Ser Ser Ile Ser Lys
355 360 365
Thr Phe Phe Lys Ala Lys Ser Asp Phe Val Lys Gln Pro Ile Pro Glu
370 375 380
Ser Ala Phe Glu Gly Leu Trp Ser Lys Phe Tyr Glu Asp Glu Ala Lys
385 390 395 400
Ser Ala Ile Met Val Phe Val Ala Phe Gly Gly Lys Met Asp Glu Ile
405 410 415
Leu Glu Thr Glu Tyr Pro Tyr Pro His Arg Ala Gly Asn Leu Tyr Thr
420 425 430
Ile Leu Tyr Thr Val Asp Trp Glu Glu Lys Glu Asn Ile Asn Ser Glu
435 440 445
Lys Phe Met Ser Trp Met Arg Arg Val Tyr Asn Tyr Met Thr Pro Tyr
450 455 460
Val Ser Lys Ser Pro Arg Glu Ala Tyr Ile Asn Tyr Arg Asp Leu Asp
465 470 475 480
Ile Gly Thr Asn Lys Ile Asn Asn Arg Lys Arg Ser Tyr Ala Lys Ala
485 490 495
Arg Val Trp Gly Val Lys Tyr Phe Lys Asn Asn Phe Asn Arg Leu Val
500 505 510
Asn Val Lys Thr Ile Val Asp Pro Gln Asn Phe Phe Arg His Glu Gln
515 520 525
Ser Ile Pro Pro Leu Phe Leu Ser Trp Lys Arg Ser Asp His Lys Lys
530 535 540
His Leu Ser Ser Trp Ser His Pro Gln Phe Glu Lys
545 550 555
<210> 104
<211> 1878
<212> DNA
<213> 人工序列
<220>
<223> CBS133-A0A1U8MHW2-全长alpha因子-无信号肽-strep标签
<220>
<221> CDS
<222> (1)..(1878)
<400> 104
atg aga ttt cct tca att ttt act gct gtt tta ttc gca gca tcc tcc 48
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser
1 5 10 15
gca tta gct gct cca gtc aac act aca aca gaa gat gaa acg gca caa 96
Ala Leu Ala Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln
20 25 30
att ccg gct gaa gct gtc atc ggt tac tca gat tta gaa ggg gat ttc 144
Ile Pro Ala Glu Ala Val Ile Gly Tyr Ser Asp Leu Glu Gly Asp Phe
35 40 45
gat gtt gct gtt ttg cca ttt tcc aac agc aca aat aac ggg tta ttg 192
Asp Val Ala Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu
50 55 60
ttt ata aat act act att gcc agc att gct gct aaa gaa gaa ggg gta 240
Phe Ile Asn Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val
65 70 75 80
tct ctc gag aaa aga gag gct gaa gct atg gct ttg cca gtt caa gaa 288
Ser Leu Glu Lys Arg Glu Ala Glu Ala Met Ala Leu Pro Val Gln Glu
85 90 95
ttc cta cat tgc ttg tct ttg aga tcc cca aac tct tct att gcc aag 336
Phe Leu His Cys Leu Ser Leu Arg Ser Pro Asn Ser Ser Ile Ala Lys
100 105 110
ttg gtc tat acc cag aac aac tct tct tac tcc tct gtt ttg aaa tcc 384
Leu Val Tyr Thr Gln Asn Asn Ser Ser Tyr Ser Ser Val Leu Lys Ser
115 120 125
gct gct caa aac ttc aga ttc act act cca tct act cca act cca ttg 432
Ala Ala Gln Asn Phe Arg Phe Thr Thr Pro Ser Thr Pro Thr Pro Leu
130 135 140
gct ata gtt act cca ttg cat gcc tct cat att caa gct gct gtt tac 480
Ala Ile Val Thr Pro Leu His Ala Ser His Ile Gln Ala Ala Val Tyr
145 150 155 160
tgt tgt aga aaa cac ggt tta caa gtc aga act aga tct ggt ggt cat 528
Cys Cys Arg Lys His Gly Leu Gln Val Arg Thr Arg Ser Gly Gly His
165 170 175
gat ttt gaa ggt ttg tct tac act tcc gct tac aag gtt cca ttc gtt 576
Asp Phe Glu Gly Leu Ser Tyr Thr Ser Ala Tyr Lys Val Pro Phe Val
180 185 190
gtt atc gat ttg gtc aac ttg aga tcc gtt aga gtt aac gtt gaa aaa 624
Val Ile Asp Leu Val Asn Leu Arg Ser Val Arg Val Asn Val Glu Lys
195 200 205
gct act gct tgg gtt gaa tct ggt gct act gtt ggt gaa tta tac gac 672
Ala Thr Ala Trp Val Glu Ser Gly Ala Thr Val Gly Glu Leu Tyr Asp
210 215 220
gaa att gcc aga aag tct aag acc ttg ggt ttt cca gct ggt att tgt 720
Glu Ile Ala Arg Lys Ser Lys Thr Leu Gly Phe Pro Ala Gly Ile Cys
225 230 235 240
cca act tta ggt gtt ggt ggt cac ttg tct ggt ggt ggt tat ggt ttg 768
Pro Thr Leu Gly Val Gly Gly His Leu Ser Gly Gly Gly Tyr Gly Leu
245 250 255
ttg ttt aga aag tat ggt ttg ggt gcc gat aac gtc att gat gct aga 816
Leu Phe Arg Lys Tyr Gly Leu Gly Ala Asp Asn Val Ile Asp Ala Arg
260 265 270
ttg att gat gtc aag ggt cgt atc ttg gac aaa aag tct atg ggt gaa 864
Leu Ile Asp Val Lys Gly Arg Ile Leu Asp Lys Lys Ser Met Gly Glu
275 280 285
gat ttg ttt tgg gcc att aga ggt ggt ggt ggc ggt act ttt ggt ata 912
Asp Leu Phe Trp Ala Ile Arg Gly Gly Gly Gly Gly Thr Phe Gly Ile
290 295 300
gtt ttg gct tgg aaa ttg aag ttg gtt gct gtt cca gct aac gtt act 960
Val Leu Ala Trp Lys Leu Lys Leu Val Ala Val Pro Ala Asn Val Thr
305 310 315 320
gtt ttc act gtt aga aag act ttg gaa caa aac gcc act aag ttg gtt 1008
Val Phe Thr Val Arg Lys Thr Leu Glu Gln Asn Ala Thr Lys Leu Val
325 330 335
cat aga tgg caa tct att gcc cac aag ttc cca aaa gaa atg cac ttg 1056
His Arg Trp Gln Ser Ile Ala His Lys Phe Pro Lys Glu Met His Leu
340 345 350
tct att gct att gct agg gct aac tct tcc gaa gat gaa gaa aag atg 1104
Ser Ile Ala Ile Ala Arg Ala Asn Ser Ser Glu Asp Glu Glu Lys Met
355 360 365
act atc caa gct tct ttc acc tct act ttc ttg ggt tcc att gat gaa 1152
Thr Ile Gln Ala Ser Phe Thr Ser Thr Phe Leu Gly Ser Ile Asp Glu
370 375 380
ttg atc cca ttg atg gaa gaa aag ttc cca gaa ttg ggt tta gtc aaa 1200
Leu Ile Pro Leu Met Glu Glu Lys Phe Pro Glu Leu Gly Leu Val Lys
385 390 395 400
gaa gat tgc cta gaa atg tct tgg gct gag tct aac ttg tac agc ttt 1248
Glu Asp Cys Leu Glu Met Ser Trp Ala Glu Ser Asn Leu Tyr Ser Phe
405 410 415
ttg ttc ttg ctg aga tct cca tct gaa acc ctg ttg aat aga aac cag 1296
Leu Phe Leu Leu Arg Ser Pro Ser Glu Thr Leu Leu Asn Arg Asn Gln
420 425 430
aag tcc tct atc tcc aaa acc ttt ttc aag gcc aag tcc gat ttt gtc 1344
Lys Ser Ser Ile Ser Lys Thr Phe Phe Lys Ala Lys Ser Asp Phe Val
435 440 445
aaa cag cca att cca gaa tct gct ttc gaa ggt tta tgg tcc aag ttt 1392
Lys Gln Pro Ile Pro Glu Ser Ala Phe Glu Gly Leu Trp Ser Lys Phe
450 455 460
tat gaa gat gag gct aag tcc gcc atc atg gtt ttt gtt gct ttt ggt 1440
Tyr Glu Asp Glu Ala Lys Ser Ala Ile Met Val Phe Val Ala Phe Gly
465 470 475 480
ggt aag atg gac gaa atc ttg gaa act gaa tac cca tat cca cat aga 1488
Gly Lys Met Asp Glu Ile Leu Glu Thr Glu Tyr Pro Tyr Pro His Arg
485 490 495
gcc ggt aac tta tac act atc ttg tac act gtt gac tgg gaa gag aaa 1536
Ala Gly Asn Leu Tyr Thr Ile Leu Tyr Thr Val Asp Trp Glu Glu Lys
500 505 510
gag aac atc aac tct gaa aag ttc atg tcc tgg atg aga aga gtc tac 1584
Glu Asn Ile Asn Ser Glu Lys Phe Met Ser Trp Met Arg Arg Val Tyr
515 520 525
aat tac atg act ccc tac gtt tct aag tct cca aga gaa gct tac atc 1632
Asn Tyr Met Thr Pro Tyr Val Ser Lys Ser Pro Arg Glu Ala Tyr Ile
530 535 540
aac tac aga gat ttg gac atc ggt aca aac aag atc aac aac aga aag 1680
Asn Tyr Arg Asp Leu Asp Ile Gly Thr Asn Lys Ile Asn Asn Arg Lys
545 550 555 560
aga tct tac gct aag gct aga gta tgg ggt gtt aag tac ttt aag aac 1728
Arg Ser Tyr Ala Lys Ala Arg Val Trp Gly Val Lys Tyr Phe Lys Asn
565 570 575
aac ttc aac agg ctg gtt aac gtc aag act ata gtt gac cca cag aat 1776
Asn Phe Asn Arg Leu Val Asn Val Lys Thr Ile Val Asp Pro Gln Asn
580 585 590
ttc ttc aga cac gaa caa tct att cca cca cta ttc ttg tcc tgg aaa 1824
Phe Phe Arg His Glu Gln Ser Ile Pro Pro Leu Phe Leu Ser Trp Lys
595 600 605
aga tcc gat cat aag aag cac ttg tcc tcc tgg tca cat cca caa ttt 1872
Arg Ser Asp His Lys Lys His Leu Ser Ser Trp Ser His Pro Gln Phe
610 615 620
gaa aag 1878
Glu Lys
625
<210> 105
<211> 626
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 105
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser
1 5 10 15
Ala Leu Ala Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln
20 25 30
Ile Pro Ala Glu Ala Val Ile Gly Tyr Ser Asp Leu Glu Gly Asp Phe
35 40 45
Asp Val Ala Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu
50 55 60
Phe Ile Asn Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val
65 70 75 80
Ser Leu Glu Lys Arg Glu Ala Glu Ala Met Ala Leu Pro Val Gln Glu
85 90 95
Phe Leu His Cys Leu Ser Leu Arg Ser Pro Asn Ser Ser Ile Ala Lys
100 105 110
Leu Val Tyr Thr Gln Asn Asn Ser Ser Tyr Ser Ser Val Leu Lys Ser
115 120 125
Ala Ala Gln Asn Phe Arg Phe Thr Thr Pro Ser Thr Pro Thr Pro Leu
130 135 140
Ala Ile Val Thr Pro Leu His Ala Ser His Ile Gln Ala Ala Val Tyr
145 150 155 160
Cys Cys Arg Lys His Gly Leu Gln Val Arg Thr Arg Ser Gly Gly His
165 170 175
Asp Phe Glu Gly Leu Ser Tyr Thr Ser Ala Tyr Lys Val Pro Phe Val
180 185 190
Val Ile Asp Leu Val Asn Leu Arg Ser Val Arg Val Asn Val Glu Lys
195 200 205
Ala Thr Ala Trp Val Glu Ser Gly Ala Thr Val Gly Glu Leu Tyr Asp
210 215 220
Glu Ile Ala Arg Lys Ser Lys Thr Leu Gly Phe Pro Ala Gly Ile Cys
225 230 235 240
Pro Thr Leu Gly Val Gly Gly His Leu Ser Gly Gly Gly Tyr Gly Leu
245 250 255
Leu Phe Arg Lys Tyr Gly Leu Gly Ala Asp Asn Val Ile Asp Ala Arg
260 265 270
Leu Ile Asp Val Lys Gly Arg Ile Leu Asp Lys Lys Ser Met Gly Glu
275 280 285
Asp Leu Phe Trp Ala Ile Arg Gly Gly Gly Gly Gly Thr Phe Gly Ile
290 295 300
Val Leu Ala Trp Lys Leu Lys Leu Val Ala Val Pro Ala Asn Val Thr
305 310 315 320
Val Phe Thr Val Arg Lys Thr Leu Glu Gln Asn Ala Thr Lys Leu Val
325 330 335
His Arg Trp Gln Ser Ile Ala His Lys Phe Pro Lys Glu Met His Leu
340 345 350
Ser Ile Ala Ile Ala Arg Ala Asn Ser Ser Glu Asp Glu Glu Lys Met
355 360 365
Thr Ile Gln Ala Ser Phe Thr Ser Thr Phe Leu Gly Ser Ile Asp Glu
370 375 380
Leu Ile Pro Leu Met Glu Glu Lys Phe Pro Glu Leu Gly Leu Val Lys
385 390 395 400
Glu Asp Cys Leu Glu Met Ser Trp Ala Glu Ser Asn Leu Tyr Ser Phe
405 410 415
Leu Phe Leu Leu Arg Ser Pro Ser Glu Thr Leu Leu Asn Arg Asn Gln
420 425 430
Lys Ser Ser Ile Ser Lys Thr Phe Phe Lys Ala Lys Ser Asp Phe Val
435 440 445
Lys Gln Pro Ile Pro Glu Ser Ala Phe Glu Gly Leu Trp Ser Lys Phe
450 455 460
Tyr Glu Asp Glu Ala Lys Ser Ala Ile Met Val Phe Val Ala Phe Gly
465 470 475 480
Gly Lys Met Asp Glu Ile Leu Glu Thr Glu Tyr Pro Tyr Pro His Arg
485 490 495
Ala Gly Asn Leu Tyr Thr Ile Leu Tyr Thr Val Asp Trp Glu Glu Lys
500 505 510
Glu Asn Ile Asn Ser Glu Lys Phe Met Ser Trp Met Arg Arg Val Tyr
515 520 525
Asn Tyr Met Thr Pro Tyr Val Ser Lys Ser Pro Arg Glu Ala Tyr Ile
530 535 540
Asn Tyr Arg Asp Leu Asp Ile Gly Thr Asn Lys Ile Asn Asn Arg Lys
545 550 555 560
Arg Ser Tyr Ala Lys Ala Arg Val Trp Gly Val Lys Tyr Phe Lys Asn
565 570 575
Asn Phe Asn Arg Leu Val Asn Val Lys Thr Ile Val Asp Pro Gln Asn
580 585 590
Phe Phe Arg His Glu Gln Ser Ile Pro Pro Leu Phe Leu Ser Trp Lys
595 600 605
Arg Ser Asp His Lys Lys His Leu Ser Ser Trp Ser His Pro Gln Phe
610 615 620
Glu Lys
625
<210> 106
<211> 1638
<212> DNA
<213> 人工序列
<220>
<223> CBS134-A0A1J3CT99
<220>
<221> CDS
<222> (1)..(1638)
<400> 106
atg agg atc tct aag cca att cca gtc atc tcc tac att ttg ttc ttg 48
Met Arg Ile Ser Lys Pro Ile Pro Val Ile Ser Tyr Ile Leu Phe Leu
1 5 10 15
gcc caa tac ttc tca ttg cac aca aaa gaa cct acc tct tct acc tca 96
Ala Gln Tyr Phe Ser Leu His Thr Lys Glu Pro Thr Ser Ser Thr Ser
20 25 30
ttg caa gat gat ttc atc aag tgc ttg cac aga aac acc aat gtt gat 144
Leu Gln Asp Asp Phe Ile Lys Cys Leu His Arg Asn Thr Asn Val Asp
35 40 45
ttc cca ttg gac aag act ttc ttc act cca caa aga aac tcc tcc aac 192
Phe Pro Leu Asp Lys Thr Phe Phe Thr Pro Gln Arg Asn Ser Ser Asn
50 55 60
ttc aaa gaa gtt ttg aac acc acc gtc tgg aat caa aga tat atg act 240
Phe Lys Glu Val Leu Asn Thr Thr Val Trp Asn Gln Arg Tyr Met Thr
65 70 75 80
aag tct atg ccc aag cca gag ttc att ttt aag cca gtt cat gaa tcc 288
Lys Ser Met Pro Lys Pro Glu Phe Ile Phe Lys Pro Val His Glu Ser
85 90 95
cat gtc caa gcc tct att att tgc tct aag aag ttg aga atc cac ttc 336
His Val Gln Ala Ser Ile Ile Cys Ser Lys Lys Leu Arg Ile His Phe
100 105 110
aga gtt aga tct ggt ggt cat gat tat gag ggt ttg tct tac gtt tct 384
Arg Val Arg Ser Gly Gly His Asp Tyr Glu Gly Leu Ser Tyr Val Ser
115 120 125
caa atg gaa act cca ttc gtc ttg atc gat ttg tgg aag ttg aag caa 432
Gln Met Glu Thr Pro Phe Val Leu Ile Asp Leu Trp Lys Leu Lys Gln
130 135 140
atc aac gtc gac atc gaa gat aat tct gct tgg gtt caa gct ggt gct 480
Ile Asn Val Asp Ile Glu Asp Asn Ser Ala Trp Val Gln Ala Gly Ala
145 150 155 160
act act ggt gag ttg tat tat aga att gct gcc aag tct aag gtt cat 528
Thr Thr Gly Glu Leu Tyr Tyr Arg Ile Ala Ala Lys Ser Lys Val His
165 170 175
ggt ttt cca gct ggt ttg tgt tct act gtt ggt ata ggt ggt cat atc 576
Gly Phe Pro Ala Gly Leu Cys Ser Thr Val Gly Ile Gly Gly His Ile
180 185 190
act ggt ggt ggt tat ggt act ttg atg aga aag tat ggt ttg tcc acc 624
Thr Gly Gly Gly Tyr Gly Thr Leu Met Arg Lys Tyr Gly Leu Ser Thr
195 200 205
gat aac gtt ttg gat gct aga atc gtt gat gct aat ggt cag ttg ttg 672
Asp Asn Val Leu Asp Ala Arg Ile Val Asp Ala Asn Gly Gln Leu Leu
210 215 220
aac aga act gct atg ggt gaa gat ttg ttc tgg gct att aga ggt ggt 720
Asn Arg Thr Ala Met Gly Glu Asp Leu Phe Trp Ala Ile Arg Gly Gly
225 230 235 240
ggt ggc ggt tca ttt ggt gtt att ttg gct tgg aag atc aag ttg gtt 768
Gly Gly Gly Ser Phe Gly Val Ile Leu Ala Trp Lys Ile Lys Leu Val
245 250 255
cca gtt cca gaa act ttg acc att ttc act att acc aag acc ttg gaa 816
Pro Val Pro Glu Thr Leu Thr Ile Phe Thr Ile Thr Lys Thr Leu Glu
260 265 270
caa gat gcc ggt ttc aag att ttg tct aag tgg caa aga atc gtc gac 864
Gln Asp Ala Gly Phe Lys Ile Leu Ser Lys Trp Gln Arg Ile Val Asp
275 280 285
aag ttg gta gaa gaa ttg tcc atc aga gtc ttg ttc aga aac act ggt 912
Lys Leu Val Glu Glu Leu Ser Ile Arg Val Leu Phe Arg Asn Thr Gly
290 295 300
aac aag act gtt act act acc tac aga ggt gaa ttc ttg ggt gaa aaa 960
Asn Lys Thr Val Thr Thr Thr Tyr Arg Gly Glu Phe Leu Gly Glu Lys
305 310 315 320
ggt aca ttg atg gaa gtc atg aag aag tgc ttt cca gaa ttg ggt ttg 1008
Gly Thr Leu Met Glu Val Met Lys Lys Cys Phe Pro Glu Leu Gly Leu
325 330 335
act caa gag gat tgc att gaa atg tct tgg atc gaa act gtt gtc aac 1056
Thr Gln Glu Asp Cys Ile Glu Met Ser Trp Ile Glu Thr Val Val Asn
340 345 350
aat gct ggt ttt act act aga cca cct cca cca ttg gaa att ttg ttg 1104
Asn Ala Gly Phe Thr Thr Arg Pro Pro Pro Pro Leu Glu Ile Leu Leu
355 360 365
caa cca tct tct cca tac ggt aga cca tac ttc aaa gct aag tct gat 1152
Gln Pro Ser Ser Pro Tyr Gly Arg Pro Tyr Phe Lys Ala Lys Ser Asp
370 375 380
ttc gtc aaa gaa ccc att cca gtt ttg ggt tta aag ggt atg ttc aag 1200
Phe Val Lys Glu Pro Ile Pro Val Leu Gly Leu Lys Gly Met Phe Lys
385 390 395 400
aga atg atc gaa gag gat gcc tct ttc ttg att tgg aaa cca tac ggt 1248
Arg Met Ile Glu Glu Asp Ala Ser Phe Leu Ile Trp Lys Pro Tyr Gly
405 410 415
ggt atg atg gct aga att cca gaa tcc gaa att cca ttt cca cac aga 1296
Gly Met Met Ala Arg Ile Pro Glu Ser Glu Ile Pro Phe Pro His Arg
420 425 430
aac ggt act atc ttc aag atc cat tac ttc acc aag tgg tct gaa aac 1344
Asn Gly Thr Ile Phe Lys Ile His Tyr Phe Thr Lys Trp Ser Glu Asn
435 440 445
ggt tct tct tct gat gac aaa cac atg aag tgg atc agg gaa ctg tat 1392
Gly Ser Ser Ser Asp Asp Lys His Met Lys Trp Ile Arg Glu Leu Tyr
450 455 460
tct tat atg acc cca tac gtt tct tct aac cca aga caa gct tac gtt 1440
Ser Tyr Met Thr Pro Tyr Val Ser Ser Asn Pro Arg Gln Ala Tyr Val
465 470 475 480
aac tac aga gat ttg gac atc ggt cag aac aag aag aat gac tct gaa 1488
Asn Tyr Arg Asp Leu Asp Ile Gly Gln Asn Lys Lys Asn Asp Ser Glu
485 490 495
gat aac ttt aga agg gct caa att tgg ggt gct aag tac ttt aag aac 1536
Asp Asn Phe Arg Arg Ala Gln Ile Trp Gly Ala Lys Tyr Phe Lys Asn
500 505 510
aac ttc aac agg ctg gtc aag atc aag aca aaa gtt gat cca gaa aac 1584
Asn Phe Asn Arg Leu Val Lys Ile Lys Thr Lys Val Asp Pro Glu Asn
515 520 525
ttg ttc agg cac gaa caa tct att cca cca tta cca att aga agg cag 1632
Leu Phe Arg His Glu Gln Ser Ile Pro Pro Leu Pro Ile Arg Arg Gln
530 535 540
acc tct 1638
Thr Ser
545
<210> 107
<211> 546
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 107
Met Arg Ile Ser Lys Pro Ile Pro Val Ile Ser Tyr Ile Leu Phe Leu
1 5 10 15
Ala Gln Tyr Phe Ser Leu His Thr Lys Glu Pro Thr Ser Ser Thr Ser
20 25 30
Leu Gln Asp Asp Phe Ile Lys Cys Leu His Arg Asn Thr Asn Val Asp
35 40 45
Phe Pro Leu Asp Lys Thr Phe Phe Thr Pro Gln Arg Asn Ser Ser Asn
50 55 60
Phe Lys Glu Val Leu Asn Thr Thr Val Trp Asn Gln Arg Tyr Met Thr
65 70 75 80
Lys Ser Met Pro Lys Pro Glu Phe Ile Phe Lys Pro Val His Glu Ser
85 90 95
His Val Gln Ala Ser Ile Ile Cys Ser Lys Lys Leu Arg Ile His Phe
100 105 110
Arg Val Arg Ser Gly Gly His Asp Tyr Glu Gly Leu Ser Tyr Val Ser
115 120 125
Gln Met Glu Thr Pro Phe Val Leu Ile Asp Leu Trp Lys Leu Lys Gln
130 135 140
Ile Asn Val Asp Ile Glu Asp Asn Ser Ala Trp Val Gln Ala Gly Ala
145 150 155 160
Thr Thr Gly Glu Leu Tyr Tyr Arg Ile Ala Ala Lys Ser Lys Val His
165 170 175
Gly Phe Pro Ala Gly Leu Cys Ser Thr Val Gly Ile Gly Gly His Ile
180 185 190
Thr Gly Gly Gly Tyr Gly Thr Leu Met Arg Lys Tyr Gly Leu Ser Thr
195 200 205
Asp Asn Val Leu Asp Ala Arg Ile Val Asp Ala Asn Gly Gln Leu Leu
210 215 220
Asn Arg Thr Ala Met Gly Glu Asp Leu Phe Trp Ala Ile Arg Gly Gly
225 230 235 240
Gly Gly Gly Ser Phe Gly Val Ile Leu Ala Trp Lys Ile Lys Leu Val
245 250 255
Pro Val Pro Glu Thr Leu Thr Ile Phe Thr Ile Thr Lys Thr Leu Glu
260 265 270
Gln Asp Ala Gly Phe Lys Ile Leu Ser Lys Trp Gln Arg Ile Val Asp
275 280 285
Lys Leu Val Glu Glu Leu Ser Ile Arg Val Leu Phe Arg Asn Thr Gly
290 295 300
Asn Lys Thr Val Thr Thr Thr Tyr Arg Gly Glu Phe Leu Gly Glu Lys
305 310 315 320
Gly Thr Leu Met Glu Val Met Lys Lys Cys Phe Pro Glu Leu Gly Leu
325 330 335
Thr Gln Glu Asp Cys Ile Glu Met Ser Trp Ile Glu Thr Val Val Asn
340 345 350
Asn Ala Gly Phe Thr Thr Arg Pro Pro Pro Pro Leu Glu Ile Leu Leu
355 360 365
Gln Pro Ser Ser Pro Tyr Gly Arg Pro Tyr Phe Lys Ala Lys Ser Asp
370 375 380
Phe Val Lys Glu Pro Ile Pro Val Leu Gly Leu Lys Gly Met Phe Lys
385 390 395 400
Arg Met Ile Glu Glu Asp Ala Ser Phe Leu Ile Trp Lys Pro Tyr Gly
405 410 415
Gly Met Met Ala Arg Ile Pro Glu Ser Glu Ile Pro Phe Pro His Arg
420 425 430
Asn Gly Thr Ile Phe Lys Ile His Tyr Phe Thr Lys Trp Ser Glu Asn
435 440 445
Gly Ser Ser Ser Asp Asp Lys His Met Lys Trp Ile Arg Glu Leu Tyr
450 455 460
Ser Tyr Met Thr Pro Tyr Val Ser Ser Asn Pro Arg Gln Ala Tyr Val
465 470 475 480
Asn Tyr Arg Asp Leu Asp Ile Gly Gln Asn Lys Lys Asn Asp Ser Glu
485 490 495
Asp Asn Phe Arg Arg Ala Gln Ile Trp Gly Ala Lys Tyr Phe Lys Asn
500 505 510
Asn Phe Asn Arg Leu Val Lys Ile Lys Thr Lys Val Asp Pro Glu Asn
515 520 525
Leu Phe Arg His Glu Gln Ser Ile Pro Pro Leu Pro Ile Arg Arg Gln
530 535 540
Thr Ser
545
<210> 108
<211> 1569
<212> DNA
<213> 人工序列
<220>
<223> CBS134-A0A1J3CT99-无信号肽
<220>
<221> CDS
<222> (1)..(1569)
<400> 108
atg aaa gaa cct acc tct tct acc tca ttg caa gat gat ttc atc aag 48
Met Lys Glu Pro Thr Ser Ser Thr Ser Leu Gln Asp Asp Phe Ile Lys
1 5 10 15
tgc ttg cac aga aac acc aat gtt gat ttc cca ttg gac aag act ttc 96
Cys Leu His Arg Asn Thr Asn Val Asp Phe Pro Leu Asp Lys Thr Phe
20 25 30
ttc act cca caa aga aac tcc tcc aac ttc aaa gaa gtt ttg aac acc 144
Phe Thr Pro Gln Arg Asn Ser Ser Asn Phe Lys Glu Val Leu Asn Thr
35 40 45
acc gtc tgg aat caa aga tat atg act aag tct atg ccc aag cca gag 192
Thr Val Trp Asn Gln Arg Tyr Met Thr Lys Ser Met Pro Lys Pro Glu
50 55 60
ttc att ttt aag cca gtt cat gaa tcc cat gtc caa gcc tct att att 240
Phe Ile Phe Lys Pro Val His Glu Ser His Val Gln Ala Ser Ile Ile
65 70 75 80
tgc tct aag aag ttg aga atc cac ttc aga gtt aga tct ggt ggt cat 288
Cys Ser Lys Lys Leu Arg Ile His Phe Arg Val Arg Ser Gly Gly His
85 90 95
gat tat gag ggt ttg tct tac gtt tct caa atg gaa act cca ttc gtc 336
Asp Tyr Glu Gly Leu Ser Tyr Val Ser Gln Met Glu Thr Pro Phe Val
100 105 110
ttg atc gat ttg tgg aag ttg aag caa atc aac gtc gac atc gaa gat 384
Leu Ile Asp Leu Trp Lys Leu Lys Gln Ile Asn Val Asp Ile Glu Asp
115 120 125
aat tct gct tgg gtt caa gct ggt gct act act ggt gag ttg tat tat 432
Asn Ser Ala Trp Val Gln Ala Gly Ala Thr Thr Gly Glu Leu Tyr Tyr
130 135 140
aga att gct gcc aag tct aag gtt cat ggt ttt cca gct ggt ttg tgt 480
Arg Ile Ala Ala Lys Ser Lys Val His Gly Phe Pro Ala Gly Leu Cys
145 150 155 160
tct act gtt ggt ata ggt ggt cat atc act ggt ggt ggt tat ggt act 528
Ser Thr Val Gly Ile Gly Gly His Ile Thr Gly Gly Gly Tyr Gly Thr
165 170 175
ttg atg aga aag tat ggt ttg tcc acc gat aac gtt ttg gat gct aga 576
Leu Met Arg Lys Tyr Gly Leu Ser Thr Asp Asn Val Leu Asp Ala Arg
180 185 190
atc gtt gat gct aat ggt cag ttg ttg aac aga act gct atg ggt gaa 624
Ile Val Asp Ala Asn Gly Gln Leu Leu Asn Arg Thr Ala Met Gly Glu
195 200 205
gat ttg ttc tgg gct att aga ggt ggt ggt ggc ggt tca ttt ggt gtt 672
Asp Leu Phe Trp Ala Ile Arg Gly Gly Gly Gly Gly Ser Phe Gly Val
210 215 220
att ttg gct tgg aag atc aag ttg gtt cca gtt cca gaa act ttg acc 720
Ile Leu Ala Trp Lys Ile Lys Leu Val Pro Val Pro Glu Thr Leu Thr
225 230 235 240
att ttc act att acc aag acc ttg gaa caa gat gcc ggt ttc aag att 768
Ile Phe Thr Ile Thr Lys Thr Leu Glu Gln Asp Ala Gly Phe Lys Ile
245 250 255
ttg tct aag tgg caa aga atc gtc gac aag ttg gta gaa gaa ttg tcc 816
Leu Ser Lys Trp Gln Arg Ile Val Asp Lys Leu Val Glu Glu Leu Ser
260 265 270
atc aga gtc ttg ttc aga aac act ggt aac aag act gtt act act acc 864
Ile Arg Val Leu Phe Arg Asn Thr Gly Asn Lys Thr Val Thr Thr Thr
275 280 285
tac aga ggt gaa ttc ttg ggt gaa aaa ggt aca ttg atg gaa gtc atg 912
Tyr Arg Gly Glu Phe Leu Gly Glu Lys Gly Thr Leu Met Glu Val Met
290 295 300
aag aag tgc ttt cca gaa ttg ggt ttg act caa gag gat tgc att gaa 960
Lys Lys Cys Phe Pro Glu Leu Gly Leu Thr Gln Glu Asp Cys Ile Glu
305 310 315 320
atg tct tgg atc gaa act gtt gtc aac aat gct ggt ttt act act aga 1008
Met Ser Trp Ile Glu Thr Val Val Asn Asn Ala Gly Phe Thr Thr Arg
325 330 335
cca cct cca cca ttg gaa att ttg ttg caa cca tct tct cca tac ggt 1056
Pro Pro Pro Pro Leu Glu Ile Leu Leu Gln Pro Ser Ser Pro Tyr Gly
340 345 350
aga cca tac ttc aaa gct aag tct gat ttc gtc aaa gaa ccc att cca 1104
Arg Pro Tyr Phe Lys Ala Lys Ser Asp Phe Val Lys Glu Pro Ile Pro
355 360 365
gtt ttg ggt tta aag ggt atg ttc aag aga atg atc gaa gag gat gcc 1152
Val Leu Gly Leu Lys Gly Met Phe Lys Arg Met Ile Glu Glu Asp Ala
370 375 380
tct ttc ttg att tgg aaa cca tac ggt ggt atg atg gct aga att cca 1200
Ser Phe Leu Ile Trp Lys Pro Tyr Gly Gly Met Met Ala Arg Ile Pro
385 390 395 400
gaa tcc gaa att cca ttt cca cac aga aac ggt act atc ttc aag atc 1248
Glu Ser Glu Ile Pro Phe Pro His Arg Asn Gly Thr Ile Phe Lys Ile
405 410 415
cat tac ttc acc aag tgg tct gaa aac ggt tct tct tct gat gac aaa 1296
His Tyr Phe Thr Lys Trp Ser Glu Asn Gly Ser Ser Ser Asp Asp Lys
420 425 430
cac atg aag tgg atc agg gaa ctg tat tct tat atg acc cca tac gtt 1344
His Met Lys Trp Ile Arg Glu Leu Tyr Ser Tyr Met Thr Pro Tyr Val
435 440 445
tct tct aac cca aga caa gct tac gtt aac tac aga gat ttg gac atc 1392
Ser Ser Asn Pro Arg Gln Ala Tyr Val Asn Tyr Arg Asp Leu Asp Ile
450 455 460
ggt cag aac aag aag aat gac tct gaa gat aac ttt aga agg gct caa 1440
Gly Gln Asn Lys Lys Asn Asp Ser Glu Asp Asn Phe Arg Arg Ala Gln
465 470 475 480
att tgg ggt gct aag tac ttt aag aac aac ttc aac agg ctg gtc aag 1488
Ile Trp Gly Ala Lys Tyr Phe Lys Asn Asn Phe Asn Arg Leu Val Lys
485 490 495
atc aag aca aaa gtt gat cca gaa aac ttg ttc agg cac gaa caa tct 1536
Ile Lys Thr Lys Val Asp Pro Glu Asn Leu Phe Arg His Glu Gln Ser
500 505 510
att cca cca tta cca att aga agg cag acc tct 1569
Ile Pro Pro Leu Pro Ile Arg Arg Gln Thr Ser
515 520
<210> 109
<211> 523
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 109
Met Lys Glu Pro Thr Ser Ser Thr Ser Leu Gln Asp Asp Phe Ile Lys
1 5 10 15
Cys Leu His Arg Asn Thr Asn Val Asp Phe Pro Leu Asp Lys Thr Phe
20 25 30
Phe Thr Pro Gln Arg Asn Ser Ser Asn Phe Lys Glu Val Leu Asn Thr
35 40 45
Thr Val Trp Asn Gln Arg Tyr Met Thr Lys Ser Met Pro Lys Pro Glu
50 55 60
Phe Ile Phe Lys Pro Val His Glu Ser His Val Gln Ala Ser Ile Ile
65 70 75 80
Cys Ser Lys Lys Leu Arg Ile His Phe Arg Val Arg Ser Gly Gly His
85 90 95
Asp Tyr Glu Gly Leu Ser Tyr Val Ser Gln Met Glu Thr Pro Phe Val
100 105 110
Leu Ile Asp Leu Trp Lys Leu Lys Gln Ile Asn Val Asp Ile Glu Asp
115 120 125
Asn Ser Ala Trp Val Gln Ala Gly Ala Thr Thr Gly Glu Leu Tyr Tyr
130 135 140
Arg Ile Ala Ala Lys Ser Lys Val His Gly Phe Pro Ala Gly Leu Cys
145 150 155 160
Ser Thr Val Gly Ile Gly Gly His Ile Thr Gly Gly Gly Tyr Gly Thr
165 170 175
Leu Met Arg Lys Tyr Gly Leu Ser Thr Asp Asn Val Leu Asp Ala Arg
180 185 190
Ile Val Asp Ala Asn Gly Gln Leu Leu Asn Arg Thr Ala Met Gly Glu
195 200 205
Asp Leu Phe Trp Ala Ile Arg Gly Gly Gly Gly Gly Ser Phe Gly Val
210 215 220
Ile Leu Ala Trp Lys Ile Lys Leu Val Pro Val Pro Glu Thr Leu Thr
225 230 235 240
Ile Phe Thr Ile Thr Lys Thr Leu Glu Gln Asp Ala Gly Phe Lys Ile
245 250 255
Leu Ser Lys Trp Gln Arg Ile Val Asp Lys Leu Val Glu Glu Leu Ser
260 265 270
Ile Arg Val Leu Phe Arg Asn Thr Gly Asn Lys Thr Val Thr Thr Thr
275 280 285
Tyr Arg Gly Glu Phe Leu Gly Glu Lys Gly Thr Leu Met Glu Val Met
290 295 300
Lys Lys Cys Phe Pro Glu Leu Gly Leu Thr Gln Glu Asp Cys Ile Glu
305 310 315 320
Met Ser Trp Ile Glu Thr Val Val Asn Asn Ala Gly Phe Thr Thr Arg
325 330 335
Pro Pro Pro Pro Leu Glu Ile Leu Leu Gln Pro Ser Ser Pro Tyr Gly
340 345 350
Arg Pro Tyr Phe Lys Ala Lys Ser Asp Phe Val Lys Glu Pro Ile Pro
355 360 365
Val Leu Gly Leu Lys Gly Met Phe Lys Arg Met Ile Glu Glu Asp Ala
370 375 380
Ser Phe Leu Ile Trp Lys Pro Tyr Gly Gly Met Met Ala Arg Ile Pro
385 390 395 400
Glu Ser Glu Ile Pro Phe Pro His Arg Asn Gly Thr Ile Phe Lys Ile
405 410 415
His Tyr Phe Thr Lys Trp Ser Glu Asn Gly Ser Ser Ser Asp Asp Lys
420 425 430
His Met Lys Trp Ile Arg Glu Leu Tyr Ser Tyr Met Thr Pro Tyr Val
435 440 445
Ser Ser Asn Pro Arg Gln Ala Tyr Val Asn Tyr Arg Asp Leu Asp Ile
450 455 460
Gly Gln Asn Lys Lys Asn Asp Ser Glu Asp Asn Phe Arg Arg Ala Gln
465 470 475 480
Ile Trp Gly Ala Lys Tyr Phe Lys Asn Asn Phe Asn Arg Leu Val Lys
485 490 495
Ile Lys Thr Lys Val Asp Pro Glu Asn Leu Phe Arg His Glu Gln Ser
500 505 510
Ile Pro Pro Leu Pro Ile Arg Arg Gln Thr Ser
515 520
<210> 110
<211> 1593
<212> DNA
<213> 人工序列
<220>
<223> CBS134-A0A1J3CT99-无信号肽-strep标签
<220>
<221> CDS
<222> (1)..(1593)
<400> 110
atg aaa gaa cct acc tct tct acc tca ttg caa gat gat ttc atc aag 48
Met Lys Glu Pro Thr Ser Ser Thr Ser Leu Gln Asp Asp Phe Ile Lys
1 5 10 15
tgc ttg cac aga aac acc aat gtt gat ttc cca ttg gac aag act ttc 96
Cys Leu His Arg Asn Thr Asn Val Asp Phe Pro Leu Asp Lys Thr Phe
20 25 30
ttc act cca caa aga aac tcc tcc aac ttc aaa gaa gtt ttg aac acc 144
Phe Thr Pro Gln Arg Asn Ser Ser Asn Phe Lys Glu Val Leu Asn Thr
35 40 45
acc gtc tgg aat caa aga tat atg act aag tct atg ccc aag cca gag 192
Thr Val Trp Asn Gln Arg Tyr Met Thr Lys Ser Met Pro Lys Pro Glu
50 55 60
ttc att ttt aag cca gtt cat gaa tcc cat gtc caa gcc tct att att 240
Phe Ile Phe Lys Pro Val His Glu Ser His Val Gln Ala Ser Ile Ile
65 70 75 80
tgc tct aag aag ttg aga atc cac ttc aga gtt aga tct ggt ggt cat 288
Cys Ser Lys Lys Leu Arg Ile His Phe Arg Val Arg Ser Gly Gly His
85 90 95
gat tat gag ggt ttg tct tac gtt tct caa atg gaa act cca ttc gtc 336
Asp Tyr Glu Gly Leu Ser Tyr Val Ser Gln Met Glu Thr Pro Phe Val
100 105 110
ttg atc gat ttg tgg aag ttg aag caa atc aac gtc gac atc gaa gat 384
Leu Ile Asp Leu Trp Lys Leu Lys Gln Ile Asn Val Asp Ile Glu Asp
115 120 125
aat tct gct tgg gtt caa gct ggt gct act act ggt gag ttg tat tat 432
Asn Ser Ala Trp Val Gln Ala Gly Ala Thr Thr Gly Glu Leu Tyr Tyr
130 135 140
aga att gct gcc aag tct aag gtt cat ggt ttt cca gct ggt ttg tgt 480
Arg Ile Ala Ala Lys Ser Lys Val His Gly Phe Pro Ala Gly Leu Cys
145 150 155 160
tct act gtt ggt ata ggt ggt cat atc act ggt ggt ggt tat ggt act 528
Ser Thr Val Gly Ile Gly Gly His Ile Thr Gly Gly Gly Tyr Gly Thr
165 170 175
ttg atg aga aag tat ggt ttg tcc acc gat aac gtt ttg gat gct aga 576
Leu Met Arg Lys Tyr Gly Leu Ser Thr Asp Asn Val Leu Asp Ala Arg
180 185 190
atc gtt gat gct aat ggt cag ttg ttg aac aga act gct atg ggt gaa 624
Ile Val Asp Ala Asn Gly Gln Leu Leu Asn Arg Thr Ala Met Gly Glu
195 200 205
gat ttg ttc tgg gct att aga ggt ggt ggt ggc ggt tca ttt ggt gtt 672
Asp Leu Phe Trp Ala Ile Arg Gly Gly Gly Gly Gly Ser Phe Gly Val
210 215 220
att ttg gct tgg aag atc aag ttg gtt cca gtt cca gaa act ttg acc 720
Ile Leu Ala Trp Lys Ile Lys Leu Val Pro Val Pro Glu Thr Leu Thr
225 230 235 240
att ttc act att acc aag acc ttg gaa caa gat gcc ggt ttc aag att 768
Ile Phe Thr Ile Thr Lys Thr Leu Glu Gln Asp Ala Gly Phe Lys Ile
245 250 255
ttg tct aag tgg caa aga atc gtc gac aag ttg gta gaa gaa ttg tcc 816
Leu Ser Lys Trp Gln Arg Ile Val Asp Lys Leu Val Glu Glu Leu Ser
260 265 270
atc aga gtc ttg ttc aga aac act ggt aac aag act gtt act act acc 864
Ile Arg Val Leu Phe Arg Asn Thr Gly Asn Lys Thr Val Thr Thr Thr
275 280 285
tac aga ggt gaa ttc ttg ggt gaa aaa ggt aca ttg atg gaa gtc atg 912
Tyr Arg Gly Glu Phe Leu Gly Glu Lys Gly Thr Leu Met Glu Val Met
290 295 300
aag aag tgc ttt cca gaa ttg ggt ttg act caa gag gat tgc att gaa 960
Lys Lys Cys Phe Pro Glu Leu Gly Leu Thr Gln Glu Asp Cys Ile Glu
305 310 315 320
atg tct tgg atc gaa act gtt gtc aac aat gct ggt ttt act act aga 1008
Met Ser Trp Ile Glu Thr Val Val Asn Asn Ala Gly Phe Thr Thr Arg
325 330 335
cca cct cca cca ttg gaa att ttg ttg caa cca tct tct cca tac ggt 1056
Pro Pro Pro Pro Leu Glu Ile Leu Leu Gln Pro Ser Ser Pro Tyr Gly
340 345 350
aga cca tac ttc aaa gct aag tct gat ttc gtc aaa gaa ccc att cca 1104
Arg Pro Tyr Phe Lys Ala Lys Ser Asp Phe Val Lys Glu Pro Ile Pro
355 360 365
gtt ttg ggt tta aag ggt atg ttc aag aga atg atc gaa gag gat gcc 1152
Val Leu Gly Leu Lys Gly Met Phe Lys Arg Met Ile Glu Glu Asp Ala
370 375 380
tct ttc ttg att tgg aaa cca tac ggt ggt atg atg gct aga att cca 1200
Ser Phe Leu Ile Trp Lys Pro Tyr Gly Gly Met Met Ala Arg Ile Pro
385 390 395 400
gaa tcc gaa att cca ttt cca cac aga aac ggt act atc ttc aag atc 1248
Glu Ser Glu Ile Pro Phe Pro His Arg Asn Gly Thr Ile Phe Lys Ile
405 410 415
cat tac ttc acc aag tgg tct gaa aac ggt tct tct tct gat gac aaa 1296
His Tyr Phe Thr Lys Trp Ser Glu Asn Gly Ser Ser Ser Asp Asp Lys
420 425 430
cac atg aag tgg atc agg gaa ctg tat tct tat atg acc cca tac gtt 1344
His Met Lys Trp Ile Arg Glu Leu Tyr Ser Tyr Met Thr Pro Tyr Val
435 440 445
tct tct aac cca aga caa gct tac gtt aac tac aga gat ttg gac atc 1392
Ser Ser Asn Pro Arg Gln Ala Tyr Val Asn Tyr Arg Asp Leu Asp Ile
450 455 460
ggt cag aac aag aag aat gac tct gaa gat aac ttt aga agg gct caa 1440
Gly Gln Asn Lys Lys Asn Asp Ser Glu Asp Asn Phe Arg Arg Ala Gln
465 470 475 480
att tgg ggt gct aag tac ttt aag aac aac ttc aac agg ctg gtc aag 1488
Ile Trp Gly Ala Lys Tyr Phe Lys Asn Asn Phe Asn Arg Leu Val Lys
485 490 495
atc aag aca aaa gtt gat cca gaa aac ttg ttc agg cac gaa caa tct 1536
Ile Lys Thr Lys Val Asp Pro Glu Asn Leu Phe Arg His Glu Gln Ser
500 505 510
att cca cca tta cca att aga agg cag acc tct tgg tca cat cca caa 1584
Ile Pro Pro Leu Pro Ile Arg Arg Gln Thr Ser Trp Ser His Pro Gln
515 520 525
ttt gaa aag 1593
Phe Glu Lys
530
<210> 111
<211> 531
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 111
Met Lys Glu Pro Thr Ser Ser Thr Ser Leu Gln Asp Asp Phe Ile Lys
1 5 10 15
Cys Leu His Arg Asn Thr Asn Val Asp Phe Pro Leu Asp Lys Thr Phe
20 25 30
Phe Thr Pro Gln Arg Asn Ser Ser Asn Phe Lys Glu Val Leu Asn Thr
35 40 45
Thr Val Trp Asn Gln Arg Tyr Met Thr Lys Ser Met Pro Lys Pro Glu
50 55 60
Phe Ile Phe Lys Pro Val His Glu Ser His Val Gln Ala Ser Ile Ile
65 70 75 80
Cys Ser Lys Lys Leu Arg Ile His Phe Arg Val Arg Ser Gly Gly His
85 90 95
Asp Tyr Glu Gly Leu Ser Tyr Val Ser Gln Met Glu Thr Pro Phe Val
100 105 110
Leu Ile Asp Leu Trp Lys Leu Lys Gln Ile Asn Val Asp Ile Glu Asp
115 120 125
Asn Ser Ala Trp Val Gln Ala Gly Ala Thr Thr Gly Glu Leu Tyr Tyr
130 135 140
Arg Ile Ala Ala Lys Ser Lys Val His Gly Phe Pro Ala Gly Leu Cys
145 150 155 160
Ser Thr Val Gly Ile Gly Gly His Ile Thr Gly Gly Gly Tyr Gly Thr
165 170 175
Leu Met Arg Lys Tyr Gly Leu Ser Thr Asp Asn Val Leu Asp Ala Arg
180 185 190
Ile Val Asp Ala Asn Gly Gln Leu Leu Asn Arg Thr Ala Met Gly Glu
195 200 205
Asp Leu Phe Trp Ala Ile Arg Gly Gly Gly Gly Gly Ser Phe Gly Val
210 215 220
Ile Leu Ala Trp Lys Ile Lys Leu Val Pro Val Pro Glu Thr Leu Thr
225 230 235 240
Ile Phe Thr Ile Thr Lys Thr Leu Glu Gln Asp Ala Gly Phe Lys Ile
245 250 255
Leu Ser Lys Trp Gln Arg Ile Val Asp Lys Leu Val Glu Glu Leu Ser
260 265 270
Ile Arg Val Leu Phe Arg Asn Thr Gly Asn Lys Thr Val Thr Thr Thr
275 280 285
Tyr Arg Gly Glu Phe Leu Gly Glu Lys Gly Thr Leu Met Glu Val Met
290 295 300
Lys Lys Cys Phe Pro Glu Leu Gly Leu Thr Gln Glu Asp Cys Ile Glu
305 310 315 320
Met Ser Trp Ile Glu Thr Val Val Asn Asn Ala Gly Phe Thr Thr Arg
325 330 335
Pro Pro Pro Pro Leu Glu Ile Leu Leu Gln Pro Ser Ser Pro Tyr Gly
340 345 350
Arg Pro Tyr Phe Lys Ala Lys Ser Asp Phe Val Lys Glu Pro Ile Pro
355 360 365
Val Leu Gly Leu Lys Gly Met Phe Lys Arg Met Ile Glu Glu Asp Ala
370 375 380
Ser Phe Leu Ile Trp Lys Pro Tyr Gly Gly Met Met Ala Arg Ile Pro
385 390 395 400
Glu Ser Glu Ile Pro Phe Pro His Arg Asn Gly Thr Ile Phe Lys Ile
405 410 415
His Tyr Phe Thr Lys Trp Ser Glu Asn Gly Ser Ser Ser Asp Asp Lys
420 425 430
His Met Lys Trp Ile Arg Glu Leu Tyr Ser Tyr Met Thr Pro Tyr Val
435 440 445
Ser Ser Asn Pro Arg Gln Ala Tyr Val Asn Tyr Arg Asp Leu Asp Ile
450 455 460
Gly Gln Asn Lys Lys Asn Asp Ser Glu Asp Asn Phe Arg Arg Ala Gln
465 470 475 480
Ile Trp Gly Ala Lys Tyr Phe Lys Asn Asn Phe Asn Arg Leu Val Lys
485 490 495
Ile Lys Thr Lys Val Asp Pro Glu Asn Leu Phe Arg His Glu Gln Ser
500 505 510
Ile Pro Pro Leu Pro Ile Arg Arg Gln Thr Ser Trp Ser His Pro Gln
515 520 525
Phe Glu Lys
530
<210> 112
<211> 1650
<212> DNA
<213> 人工序列
<220>
<223> CBS134-A0A1J3CT99-截断alpha因子-无信号肽-strep标签
<220>
<221> CDS
<222> (1)..(1650)
<400> 112
atg aga ttt cct tcc atc ttc acg gct gtg cta ttt gca gca tcc tcc 48
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser
1 5 10 15
gca ctt gca atg aaa gaa cct acc tct tct acc tca ttg caa gat gat 96
Ala Leu Ala Met Lys Glu Pro Thr Ser Ser Thr Ser Leu Gln Asp Asp
20 25 30
ttc atc aag tgc ttg cac aga aac acc aat gtt gat ttc cca ttg gac 144
Phe Ile Lys Cys Leu His Arg Asn Thr Asn Val Asp Phe Pro Leu Asp
35 40 45
aag act ttc ttc act cca caa aga aac tcc tcc aac ttc aaa gaa gtt 192
Lys Thr Phe Phe Thr Pro Gln Arg Asn Ser Ser Asn Phe Lys Glu Val
50 55 60
ttg aac acc acc gtc tgg aat caa aga tat atg act aag tct atg ccc 240
Leu Asn Thr Thr Val Trp Asn Gln Arg Tyr Met Thr Lys Ser Met Pro
65 70 75 80
aag cca gag ttc att ttt aag cca gtt cat gaa tcc cat gtc caa gcc 288
Lys Pro Glu Phe Ile Phe Lys Pro Val His Glu Ser His Val Gln Ala
85 90 95
tct att att tgc tct aag aag ttg aga atc cac ttc aga gtt aga tct 336
Ser Ile Ile Cys Ser Lys Lys Leu Arg Ile His Phe Arg Val Arg Ser
100 105 110
ggt ggt cat gat tat gag ggt ttg tct tac gtt tct caa atg gaa act 384
Gly Gly His Asp Tyr Glu Gly Leu Ser Tyr Val Ser Gln Met Glu Thr
115 120 125
cca ttc gtc ttg atc gat ttg tgg aag ttg aag caa atc aac gtc gac 432
Pro Phe Val Leu Ile Asp Leu Trp Lys Leu Lys Gln Ile Asn Val Asp
130 135 140
atc gaa gat aat tct gct tgg gtt caa gct ggt gct act act ggt gag 480
Ile Glu Asp Asn Ser Ala Trp Val Gln Ala Gly Ala Thr Thr Gly Glu
145 150 155 160
ttg tat tat aga att gct gcc aag tct aag gtt cat ggt ttt cca gct 528
Leu Tyr Tyr Arg Ile Ala Ala Lys Ser Lys Val His Gly Phe Pro Ala
165 170 175
ggt ttg tgt tct act gtt ggt ata ggt ggt cat atc act ggt ggt ggt 576
Gly Leu Cys Ser Thr Val Gly Ile Gly Gly His Ile Thr Gly Gly Gly
180 185 190
tat ggt act ttg atg aga aag tat ggt ttg tcc acc gat aac gtt ttg 624
Tyr Gly Thr Leu Met Arg Lys Tyr Gly Leu Ser Thr Asp Asn Val Leu
195 200 205
gat gct aga atc gtt gat gct aat ggt cag ttg ttg aac aga act gct 672
Asp Ala Arg Ile Val Asp Ala Asn Gly Gln Leu Leu Asn Arg Thr Ala
210 215 220
atg ggt gaa gat ttg ttc tgg gct att aga ggt ggt ggt ggc ggt tca 720
Met Gly Glu Asp Leu Phe Trp Ala Ile Arg Gly Gly Gly Gly Gly Ser
225 230 235 240
ttt ggt gtt att ttg gct tgg aag atc aag ttg gtt cca gtt cca gaa 768
Phe Gly Val Ile Leu Ala Trp Lys Ile Lys Leu Val Pro Val Pro Glu
245 250 255
act ttg acc att ttc act att acc aag acc ttg gaa caa gat gcc ggt 816
Thr Leu Thr Ile Phe Thr Ile Thr Lys Thr Leu Glu Gln Asp Ala Gly
260 265 270
ttc aag att ttg tct aag tgg caa aga atc gtc gac aag ttg gta gaa 864
Phe Lys Ile Leu Ser Lys Trp Gln Arg Ile Val Asp Lys Leu Val Glu
275 280 285
gaa ttg tcc atc aga gtc ttg ttc aga aac act ggt aac aag act gtt 912
Glu Leu Ser Ile Arg Val Leu Phe Arg Asn Thr Gly Asn Lys Thr Val
290 295 300
act act acc tac aga ggt gaa ttc ttg ggt gaa aaa ggt aca ttg atg 960
Thr Thr Thr Tyr Arg Gly Glu Phe Leu Gly Glu Lys Gly Thr Leu Met
305 310 315 320
gaa gtc atg aag aag tgc ttt cca gaa ttg ggt ttg act caa gag gat 1008
Glu Val Met Lys Lys Cys Phe Pro Glu Leu Gly Leu Thr Gln Glu Asp
325 330 335
tgc att gaa atg tct tgg atc gaa act gtt gtc aac aat gct ggt ttt 1056
Cys Ile Glu Met Ser Trp Ile Glu Thr Val Val Asn Asn Ala Gly Phe
340 345 350
act act aga cca cct cca cca ttg gaa att ttg ttg caa cca tct tct 1104
Thr Thr Arg Pro Pro Pro Pro Leu Glu Ile Leu Leu Gln Pro Ser Ser
355 360 365
cca tac ggt aga cca tac ttc aaa gct aag tct gat ttc gtc aaa gaa 1152
Pro Tyr Gly Arg Pro Tyr Phe Lys Ala Lys Ser Asp Phe Val Lys Glu
370 375 380
ccc att cca gtt ttg ggt tta aag ggt atg ttc aag aga atg atc gaa 1200
Pro Ile Pro Val Leu Gly Leu Lys Gly Met Phe Lys Arg Met Ile Glu
385 390 395 400
gag gat gcc tct ttc ttg att tgg aaa cca tac ggt ggt atg atg gct 1248
Glu Asp Ala Ser Phe Leu Ile Trp Lys Pro Tyr Gly Gly Met Met Ala
405 410 415
aga att cca gaa tcc gaa att cca ttt cca cac aga aac ggt act atc 1296
Arg Ile Pro Glu Ser Glu Ile Pro Phe Pro His Arg Asn Gly Thr Ile
420 425 430
ttc aag atc cat tac ttc acc aag tgg tct gaa aac ggt tct tct tct 1344
Phe Lys Ile His Tyr Phe Thr Lys Trp Ser Glu Asn Gly Ser Ser Ser
435 440 445
gat gac aaa cac atg aag tgg atc agg gaa ctg tat tct tat atg acc 1392
Asp Asp Lys His Met Lys Trp Ile Arg Glu Leu Tyr Ser Tyr Met Thr
450 455 460
cca tac gtt tct tct aac cca aga caa gct tac gtt aac tac aga gat 1440
Pro Tyr Val Ser Ser Asn Pro Arg Gln Ala Tyr Val Asn Tyr Arg Asp
465 470 475 480
ttg gac atc ggt cag aac aag aag aat gac tct gaa gat aac ttt aga 1488
Leu Asp Ile Gly Gln Asn Lys Lys Asn Asp Ser Glu Asp Asn Phe Arg
485 490 495
agg gct caa att tgg ggt gct aag tac ttt aag aac aac ttc aac agg 1536
Arg Ala Gln Ile Trp Gly Ala Lys Tyr Phe Lys Asn Asn Phe Asn Arg
500 505 510
ctg gtc aag atc aag aca aaa gtt gat cca gaa aac ttg ttc agg cac 1584
Leu Val Lys Ile Lys Thr Lys Val Asp Pro Glu Asn Leu Phe Arg His
515 520 525
gaa caa tct att cca cca tta cca att aga agg cag acc tct tgg tca 1632
Glu Gln Ser Ile Pro Pro Leu Pro Ile Arg Arg Gln Thr Ser Trp Ser
530 535 540
cat cca caa ttt gaa aag 1650
His Pro Gln Phe Glu Lys
545 550
<210> 113
<211> 550
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 113
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser
1 5 10 15
Ala Leu Ala Met Lys Glu Pro Thr Ser Ser Thr Ser Leu Gln Asp Asp
20 25 30
Phe Ile Lys Cys Leu His Arg Asn Thr Asn Val Asp Phe Pro Leu Asp
35 40 45
Lys Thr Phe Phe Thr Pro Gln Arg Asn Ser Ser Asn Phe Lys Glu Val
50 55 60
Leu Asn Thr Thr Val Trp Asn Gln Arg Tyr Met Thr Lys Ser Met Pro
65 70 75 80
Lys Pro Glu Phe Ile Phe Lys Pro Val His Glu Ser His Val Gln Ala
85 90 95
Ser Ile Ile Cys Ser Lys Lys Leu Arg Ile His Phe Arg Val Arg Ser
100 105 110
Gly Gly His Asp Tyr Glu Gly Leu Ser Tyr Val Ser Gln Met Glu Thr
115 120 125
Pro Phe Val Leu Ile Asp Leu Trp Lys Leu Lys Gln Ile Asn Val Asp
130 135 140
Ile Glu Asp Asn Ser Ala Trp Val Gln Ala Gly Ala Thr Thr Gly Glu
145 150 155 160
Leu Tyr Tyr Arg Ile Ala Ala Lys Ser Lys Val His Gly Phe Pro Ala
165 170 175
Gly Leu Cys Ser Thr Val Gly Ile Gly Gly His Ile Thr Gly Gly Gly
180 185 190
Tyr Gly Thr Leu Met Arg Lys Tyr Gly Leu Ser Thr Asp Asn Val Leu
195 200 205
Asp Ala Arg Ile Val Asp Ala Asn Gly Gln Leu Leu Asn Arg Thr Ala
210 215 220
Met Gly Glu Asp Leu Phe Trp Ala Ile Arg Gly Gly Gly Gly Gly Ser
225 230 235 240
Phe Gly Val Ile Leu Ala Trp Lys Ile Lys Leu Val Pro Val Pro Glu
245 250 255
Thr Leu Thr Ile Phe Thr Ile Thr Lys Thr Leu Glu Gln Asp Ala Gly
260 265 270
Phe Lys Ile Leu Ser Lys Trp Gln Arg Ile Val Asp Lys Leu Val Glu
275 280 285
Glu Leu Ser Ile Arg Val Leu Phe Arg Asn Thr Gly Asn Lys Thr Val
290 295 300
Thr Thr Thr Tyr Arg Gly Glu Phe Leu Gly Glu Lys Gly Thr Leu Met
305 310 315 320
Glu Val Met Lys Lys Cys Phe Pro Glu Leu Gly Leu Thr Gln Glu Asp
325 330 335
Cys Ile Glu Met Ser Trp Ile Glu Thr Val Val Asn Asn Ala Gly Phe
340 345 350
Thr Thr Arg Pro Pro Pro Pro Leu Glu Ile Leu Leu Gln Pro Ser Ser
355 360 365
Pro Tyr Gly Arg Pro Tyr Phe Lys Ala Lys Ser Asp Phe Val Lys Glu
370 375 380
Pro Ile Pro Val Leu Gly Leu Lys Gly Met Phe Lys Arg Met Ile Glu
385 390 395 400
Glu Asp Ala Ser Phe Leu Ile Trp Lys Pro Tyr Gly Gly Met Met Ala
405 410 415
Arg Ile Pro Glu Ser Glu Ile Pro Phe Pro His Arg Asn Gly Thr Ile
420 425 430
Phe Lys Ile His Tyr Phe Thr Lys Trp Ser Glu Asn Gly Ser Ser Ser
435 440 445
Asp Asp Lys His Met Lys Trp Ile Arg Glu Leu Tyr Ser Tyr Met Thr
450 455 460
Pro Tyr Val Ser Ser Asn Pro Arg Gln Ala Tyr Val Asn Tyr Arg Asp
465 470 475 480
Leu Asp Ile Gly Gln Asn Lys Lys Asn Asp Ser Glu Asp Asn Phe Arg
485 490 495
Arg Ala Gln Ile Trp Gly Ala Lys Tyr Phe Lys Asn Asn Phe Asn Arg
500 505 510
Leu Val Lys Ile Lys Thr Lys Val Asp Pro Glu Asn Leu Phe Arg His
515 520 525
Glu Gln Ser Ile Pro Pro Leu Pro Ile Arg Arg Gln Thr Ser Trp Ser
530 535 540
His Pro Gln Phe Glu Lys
545 550
<210> 114
<211> 1860
<212> DNA
<213> 人工序列
<220>
<223> CBS134-A0A1J3CT99-全长alpha因子-无信号肽-strep标签
<220>
<221> CDS
<222> (1)..(1860)
<400> 114
atg aga ttt cct tca att ttt act gct gtt tta ttc gca gca tcc tcc 48
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser
1 5 10 15
gca tta gct gct cca gtc aac act aca aca gaa gat gaa acg gca caa 96
Ala Leu Ala Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln
20 25 30
att ccg gct gaa gct gtc atc ggt tac tca gat tta gaa ggg gat ttc 144
Ile Pro Ala Glu Ala Val Ile Gly Tyr Ser Asp Leu Glu Gly Asp Phe
35 40 45
gat gtt gct gtt ttg cca ttt tcc aac agc aca aat aac ggg tta ttg 192
Asp Val Ala Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu
50 55 60
ttt ata aat act act att gcc agc att gct gct aaa gaa gaa ggg gta 240
Phe Ile Asn Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val
65 70 75 80
tct ctc gag aaa aga gag gct gaa gct atg aaa gaa cct acc tct tct 288
Ser Leu Glu Lys Arg Glu Ala Glu Ala Met Lys Glu Pro Thr Ser Ser
85 90 95
acc tca ttg caa gat gat ttc atc aag tgc ttg cac aga aac acc aat 336
Thr Ser Leu Gln Asp Asp Phe Ile Lys Cys Leu His Arg Asn Thr Asn
100 105 110
gtt gat ttc cca ttg gac aag act ttc ttc act cca caa aga aac tcc 384
Val Asp Phe Pro Leu Asp Lys Thr Phe Phe Thr Pro Gln Arg Asn Ser
115 120 125
tcc aac ttc aaa gaa gtt ttg aac acc acc gtc tgg aat caa aga tat 432
Ser Asn Phe Lys Glu Val Leu Asn Thr Thr Val Trp Asn Gln Arg Tyr
130 135 140
atg act aag tct atg ccc aag cca gag ttc att ttt aag cca gtt cat 480
Met Thr Lys Ser Met Pro Lys Pro Glu Phe Ile Phe Lys Pro Val His
145 150 155 160
gaa tcc cat gtc caa gcc tct att att tgc tct aag aag ttg aga atc 528
Glu Ser His Val Gln Ala Ser Ile Ile Cys Ser Lys Lys Leu Arg Ile
165 170 175
cac ttc aga gtt aga tct ggt ggt cat gat tat gag ggt ttg tct tac 576
His Phe Arg Val Arg Ser Gly Gly His Asp Tyr Glu Gly Leu Ser Tyr
180 185 190
gtt tct caa atg gaa act cca ttc gtc ttg atc gat ttg tgg aag ttg 624
Val Ser Gln Met Glu Thr Pro Phe Val Leu Ile Asp Leu Trp Lys Leu
195 200 205
aag caa atc aac gtc gac atc gaa gat aat tct gct tgg gtt caa gct 672
Lys Gln Ile Asn Val Asp Ile Glu Asp Asn Ser Ala Trp Val Gln Ala
210 215 220
ggt gct act act ggt gag ttg tat tat aga att gct gcc aag tct aag 720
Gly Ala Thr Thr Gly Glu Leu Tyr Tyr Arg Ile Ala Ala Lys Ser Lys
225 230 235 240
gtt cat ggt ttt cca gct ggt ttg tgt tct act gtt ggt ata ggt ggt 768
Val His Gly Phe Pro Ala Gly Leu Cys Ser Thr Val Gly Ile Gly Gly
245 250 255
cat atc act ggt ggt ggt tat ggt act ttg atg aga aag tat ggt ttg 816
His Ile Thr Gly Gly Gly Tyr Gly Thr Leu Met Arg Lys Tyr Gly Leu
260 265 270
tcc acc gat aac gtt ttg gat gct aga atc gtt gat gct aat ggt cag 864
Ser Thr Asp Asn Val Leu Asp Ala Arg Ile Val Asp Ala Asn Gly Gln
275 280 285
ttg ttg aac aga act gct atg ggt gaa gat ttg ttc tgg gct att aga 912
Leu Leu Asn Arg Thr Ala Met Gly Glu Asp Leu Phe Trp Ala Ile Arg
290 295 300
ggt ggt ggt ggc ggt tca ttt ggt gtt att ttg gct tgg aag atc aag 960
Gly Gly Gly Gly Gly Ser Phe Gly Val Ile Leu Ala Trp Lys Ile Lys
305 310 315 320
ttg gtt cca gtt cca gaa act ttg acc att ttc act att acc aag acc 1008
Leu Val Pro Val Pro Glu Thr Leu Thr Ile Phe Thr Ile Thr Lys Thr
325 330 335
ttg gaa caa gat gcc ggt ttc aag att ttg tct aag tgg caa aga atc 1056
Leu Glu Gln Asp Ala Gly Phe Lys Ile Leu Ser Lys Trp Gln Arg Ile
340 345 350
gtc gac aag ttg gta gaa gaa ttg tcc atc aga gtc ttg ttc aga aac 1104
Val Asp Lys Leu Val Glu Glu Leu Ser Ile Arg Val Leu Phe Arg Asn
355 360 365
act ggt aac aag act gtt act act acc tac aga ggt gaa ttc ttg ggt 1152
Thr Gly Asn Lys Thr Val Thr Thr Thr Tyr Arg Gly Glu Phe Leu Gly
370 375 380
gaa aaa ggt aca ttg atg gaa gtc atg aag aag tgc ttt cca gaa ttg 1200
Glu Lys Gly Thr Leu Met Glu Val Met Lys Lys Cys Phe Pro Glu Leu
385 390 395 400
ggt ttg act caa gag gat tgc att gaa atg tct tgg atc gaa act gtt 1248
Gly Leu Thr Gln Glu Asp Cys Ile Glu Met Ser Trp Ile Glu Thr Val
405 410 415
gtc aac aat gct ggt ttt act act aga cca cct cca cca ttg gaa att 1296
Val Asn Asn Ala Gly Phe Thr Thr Arg Pro Pro Pro Pro Leu Glu Ile
420 425 430
ttg ttg caa cca tct tct cca tac ggt aga cca tac ttc aaa gct aag 1344
Leu Leu Gln Pro Ser Ser Pro Tyr Gly Arg Pro Tyr Phe Lys Ala Lys
435 440 445
tct gat ttc gtc aaa gaa ccc att cca gtt ttg ggt tta aag ggt atg 1392
Ser Asp Phe Val Lys Glu Pro Ile Pro Val Leu Gly Leu Lys Gly Met
450 455 460
ttc aag aga atg atc gaa gag gat gcc tct ttc ttg att tgg aaa cca 1440
Phe Lys Arg Met Ile Glu Glu Asp Ala Ser Phe Leu Ile Trp Lys Pro
465 470 475 480
tac ggt ggt atg atg gct aga att cca gaa tcc gaa att cca ttt cca 1488
Tyr Gly Gly Met Met Ala Arg Ile Pro Glu Ser Glu Ile Pro Phe Pro
485 490 495
cac aga aac ggt act atc ttc aag atc cat tac ttc acc aag tgg tct 1536
His Arg Asn Gly Thr Ile Phe Lys Ile His Tyr Phe Thr Lys Trp Ser
500 505 510
gaa aac ggt tct tct tct gat gac aaa cac atg aag tgg atc agg gaa 1584
Glu Asn Gly Ser Ser Ser Asp Asp Lys His Met Lys Trp Ile Arg Glu
515 520 525
ctg tat tct tat atg acc cca tac gtt tct tct aac cca aga caa gct 1632
Leu Tyr Ser Tyr Met Thr Pro Tyr Val Ser Ser Asn Pro Arg Gln Ala
530 535 540
tac gtt aac tac aga gat ttg gac atc ggt cag aac aag aag aat gac 1680
Tyr Val Asn Tyr Arg Asp Leu Asp Ile Gly Gln Asn Lys Lys Asn Asp
545 550 555 560
tct gaa gat aac ttt aga agg gct caa att tgg ggt gct aag tac ttt 1728
Ser Glu Asp Asn Phe Arg Arg Ala Gln Ile Trp Gly Ala Lys Tyr Phe
565 570 575
aag aac aac ttc aac agg ctg gtc aag atc aag aca aaa gtt gat cca 1776
Lys Asn Asn Phe Asn Arg Leu Val Lys Ile Lys Thr Lys Val Asp Pro
580 585 590
gaa aac ttg ttc agg cac gaa caa tct att cca cca tta cca att aga 1824
Glu Asn Leu Phe Arg His Glu Gln Ser Ile Pro Pro Leu Pro Ile Arg
595 600 605
agg cag acc tct tgg tca cat cca caa ttt gaa aag 1860
Arg Gln Thr Ser Trp Ser His Pro Gln Phe Glu Lys
610 615 620
<210> 115
<211> 620
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 115
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser
1 5 10 15
Ala Leu Ala Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln
20 25 30
Ile Pro Ala Glu Ala Val Ile Gly Tyr Ser Asp Leu Glu Gly Asp Phe
35 40 45
Asp Val Ala Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu
50 55 60
Phe Ile Asn Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val
65 70 75 80
Ser Leu Glu Lys Arg Glu Ala Glu Ala Met Lys Glu Pro Thr Ser Ser
85 90 95
Thr Ser Leu Gln Asp Asp Phe Ile Lys Cys Leu His Arg Asn Thr Asn
100 105 110
Val Asp Phe Pro Leu Asp Lys Thr Phe Phe Thr Pro Gln Arg Asn Ser
115 120 125
Ser Asn Phe Lys Glu Val Leu Asn Thr Thr Val Trp Asn Gln Arg Tyr
130 135 140
Met Thr Lys Ser Met Pro Lys Pro Glu Phe Ile Phe Lys Pro Val His
145 150 155 160
Glu Ser His Val Gln Ala Ser Ile Ile Cys Ser Lys Lys Leu Arg Ile
165 170 175
His Phe Arg Val Arg Ser Gly Gly His Asp Tyr Glu Gly Leu Ser Tyr
180 185 190
Val Ser Gln Met Glu Thr Pro Phe Val Leu Ile Asp Leu Trp Lys Leu
195 200 205
Lys Gln Ile Asn Val Asp Ile Glu Asp Asn Ser Ala Trp Val Gln Ala
210 215 220
Gly Ala Thr Thr Gly Glu Leu Tyr Tyr Arg Ile Ala Ala Lys Ser Lys
225 230 235 240
Val His Gly Phe Pro Ala Gly Leu Cys Ser Thr Val Gly Ile Gly Gly
245 250 255
His Ile Thr Gly Gly Gly Tyr Gly Thr Leu Met Arg Lys Tyr Gly Leu
260 265 270
Ser Thr Asp Asn Val Leu Asp Ala Arg Ile Val Asp Ala Asn Gly Gln
275 280 285
Leu Leu Asn Arg Thr Ala Met Gly Glu Asp Leu Phe Trp Ala Ile Arg
290 295 300
Gly Gly Gly Gly Gly Ser Phe Gly Val Ile Leu Ala Trp Lys Ile Lys
305 310 315 320
Leu Val Pro Val Pro Glu Thr Leu Thr Ile Phe Thr Ile Thr Lys Thr
325 330 335
Leu Glu Gln Asp Ala Gly Phe Lys Ile Leu Ser Lys Trp Gln Arg Ile
340 345 350
Val Asp Lys Leu Val Glu Glu Leu Ser Ile Arg Val Leu Phe Arg Asn
355 360 365
Thr Gly Asn Lys Thr Val Thr Thr Thr Tyr Arg Gly Glu Phe Leu Gly
370 375 380
Glu Lys Gly Thr Leu Met Glu Val Met Lys Lys Cys Phe Pro Glu Leu
385 390 395 400
Gly Leu Thr Gln Glu Asp Cys Ile Glu Met Ser Trp Ile Glu Thr Val
405 410 415
Val Asn Asn Ala Gly Phe Thr Thr Arg Pro Pro Pro Pro Leu Glu Ile
420 425 430
Leu Leu Gln Pro Ser Ser Pro Tyr Gly Arg Pro Tyr Phe Lys Ala Lys
435 440 445
Ser Asp Phe Val Lys Glu Pro Ile Pro Val Leu Gly Leu Lys Gly Met
450 455 460
Phe Lys Arg Met Ile Glu Glu Asp Ala Ser Phe Leu Ile Trp Lys Pro
465 470 475 480
Tyr Gly Gly Met Met Ala Arg Ile Pro Glu Ser Glu Ile Pro Phe Pro
485 490 495
His Arg Asn Gly Thr Ile Phe Lys Ile His Tyr Phe Thr Lys Trp Ser
500 505 510
Glu Asn Gly Ser Ser Ser Asp Asp Lys His Met Lys Trp Ile Arg Glu
515 520 525
Leu Tyr Ser Tyr Met Thr Pro Tyr Val Ser Ser Asn Pro Arg Gln Ala
530 535 540
Tyr Val Asn Tyr Arg Asp Leu Asp Ile Gly Gln Asn Lys Lys Asn Asp
545 550 555 560
Ser Glu Asp Asn Phe Arg Arg Ala Gln Ile Trp Gly Ala Lys Tyr Phe
565 570 575
Lys Asn Asn Phe Asn Arg Leu Val Lys Ile Lys Thr Lys Val Asp Pro
580 585 590
Glu Asn Leu Phe Arg His Glu Gln Ser Ile Pro Pro Leu Pro Ile Arg
595 600 605
Arg Gln Thr Ser Trp Ser His Pro Gln Phe Glu Lys
610 615 620
<210> 116
<211> 1638
<212> DNA
<213> 人工序列
<220>
<223> CBS146-A0A1J3J6A2
<220>
<221> CDS
<222> (1)..(1638)
<400> 116
atg agg atc tct aag cca att cca gtc atc tcc tac att ttg ttc ttg 48
Met Arg Ile Ser Lys Pro Ile Pro Val Ile Ser Tyr Ile Leu Phe Leu
1 5 10 15
gcc caa tac ttc tca ttg cac aca aaa gaa cct acc tct tct acc tca 96
Ala Gln Tyr Phe Ser Leu His Thr Lys Glu Pro Thr Ser Ser Thr Ser
20 25 30
ttg caa gat gat ttt gtt aag tgc ttg cac aga aac acc aac gtt gat 144
Leu Gln Asp Asp Phe Val Lys Cys Leu His Arg Asn Thr Asn Val Asp
35 40 45
ttt cca ttg gac aag act ttc ttc acc cca caa aga aac tcc tcc aac 192
Phe Pro Leu Asp Lys Thr Phe Phe Thr Pro Gln Arg Asn Ser Ser Asn
50 55 60
ttc aaa gaa gtc ttg aac tct acc gtc tgg aat caa aga tat atg acc 240
Phe Lys Glu Val Leu Asn Ser Thr Val Trp Asn Gln Arg Tyr Met Thr
65 70 75 80
aag tct atg cca aag cca gag ttc att ttt aag cca gtt cat gaa tcc 288
Lys Ser Met Pro Lys Pro Glu Phe Ile Phe Lys Pro Val His Glu Ser
85 90 95
cat gtc caa gcc tct att att tgc tct aag aag ttg aga atc cac ttc 336
His Val Gln Ala Ser Ile Ile Cys Ser Lys Lys Leu Arg Ile His Phe
100 105 110
aga gtt aga tct ggt ggt cat gat tat gag ggt ttg tct tac gtt tct 384
Arg Val Arg Ser Gly Gly His Asp Tyr Glu Gly Leu Ser Tyr Val Ser
115 120 125
caa atg gaa act cca ttc gtc ttg atc gat ttg tgg aag ttg aag cag 432
Gln Met Glu Thr Pro Phe Val Leu Ile Asp Leu Trp Lys Leu Lys Gln
130 135 140
att aac gtc gac atc gaa gat aat tct gct tgg gtt caa gct ggt gct 480
Ile Asn Val Asp Ile Glu Asp Asn Ser Ala Trp Val Gln Ala Gly Ala
145 150 155 160
act act ggt gag ttg tat tat aga att gcc gag aag tct aag gtt cat 528
Thr Thr Gly Glu Leu Tyr Tyr Arg Ile Ala Glu Lys Ser Lys Val His
165 170 175
ggt ttt cca gct ggt ttg tgt tct act gtt ggt ata ggt ggt cat atc 576
Gly Phe Pro Ala Gly Leu Cys Ser Thr Val Gly Ile Gly Gly His Ile
180 185 190
act ggt ggt ggt tat ggt act ttg atg aga aag tat ggt ttg tcc acc 624
Thr Gly Gly Gly Tyr Gly Thr Leu Met Arg Lys Tyr Gly Leu Ser Thr
195 200 205
gat aac gtt ttg gat gct aga atc gtt gat gct aat ggt cag ttg ttg 672
Asp Asn Val Leu Asp Ala Arg Ile Val Asp Ala Asn Gly Gln Leu Leu
210 215 220
aac aga act gct atg ggt gaa gat ttg ttc tgg gct att tct ggt ggc 720
Asn Arg Thr Ala Met Gly Glu Asp Leu Phe Trp Ala Ile Ser Gly Gly
225 230 235 240
ggt ggt ggt agt ttt ggt gtt att ttg gct tgg aag att aag ttg gtt 768
Gly Gly Gly Ser Phe Gly Val Ile Leu Ala Trp Lys Ile Lys Leu Val
245 250 255
cca gtt cca gaa acc ttg acc att ttc act att acc aag acc ttg gaa 816
Pro Val Pro Glu Thr Leu Thr Ile Phe Thr Ile Thr Lys Thr Leu Glu
260 265 270
caa gat gcc ggt ttc aag att ttg tcc aaa tgg caa aga atc gtc gac 864
Gln Asp Ala Gly Phe Lys Ile Leu Ser Lys Trp Gln Arg Ile Val Asp
275 280 285
aag ttg gtt gaa gaa ttg tcc atc aga gtc ttg ttc aga aac act ggt 912
Lys Leu Val Glu Glu Leu Ser Ile Arg Val Leu Phe Arg Asn Thr Gly
290 295 300
aac aag act gtt act act acc tac aga ggt gaa ttc ttg ggt gaa aaa 960
Asn Lys Thr Val Thr Thr Thr Tyr Arg Gly Glu Phe Leu Gly Glu Lys
305 310 315 320
ggt aca ttg atg gaa gtc atg aac aag tgc ttt cca gaa ttg ggt ttg 1008
Gly Thr Leu Met Glu Val Met Asn Lys Cys Phe Pro Glu Leu Gly Leu
325 330 335
act caa gag gat tgc att gaa atg tct tgg atc gaa act gtt gtc aac 1056
Thr Gln Glu Asp Cys Ile Glu Met Ser Trp Ile Glu Thr Val Val Asn
340 345 350
aat gct ggt ttt act act aga cca cca cct cca ttg gaa att ttg ttg 1104
Asn Ala Gly Phe Thr Thr Arg Pro Pro Pro Pro Leu Glu Ile Leu Leu
355 360 365
caa cca tct tct cca tac ggt aga cca tac ttc aaa gct aag tct gat 1152
Gln Pro Ser Ser Pro Tyr Gly Arg Pro Tyr Phe Lys Ala Lys Ser Asp
370 375 380
ttc gtc aaa gaa ccc att cca gtt ttg ggt tta aag ggt atg ttc aag 1200
Phe Val Lys Glu Pro Ile Pro Val Leu Gly Leu Lys Gly Met Phe Lys
385 390 395 400
aga atg atc gaa gag gat gcc tct ttc ttg att tgg aaa cca tac ggt 1248
Arg Met Ile Glu Glu Asp Ala Ser Phe Leu Ile Trp Lys Pro Tyr Gly
405 410 415
ggt atg atg gct aga att cca gaa tct gaa ttg cca ttt cca cac aga 1296
Gly Met Met Ala Arg Ile Pro Glu Ser Glu Leu Pro Phe Pro His Arg
420 425 430
aac ggt act att ttc aag atc cat tac ttc acc aag tgg tcc gaa aat 1344
Asn Gly Thr Ile Phe Lys Ile His Tyr Phe Thr Lys Trp Ser Glu Asn
435 440 445
ggt tct tct tct gat gac aaa cac atg aag tgg atc agg gaa ctg tat 1392
Gly Ser Ser Ser Asp Asp Lys His Met Lys Trp Ile Arg Glu Leu Tyr
450 455 460
tct tat atg acc cca tac gtt tct tct aac cca aga caa gct tac gtt 1440
Ser Tyr Met Thr Pro Tyr Val Ser Ser Asn Pro Arg Gln Ala Tyr Val
465 470 475 480
aac tac aga gat ttg gac atc ggt cag aac aag aag aac gat tct gaa 1488
Asn Tyr Arg Asp Leu Asp Ile Gly Gln Asn Lys Lys Asn Asp Ser Glu
485 490 495
aac aat ttc aga agg gct caa att tgg ggt gct aag tac ttt aag aac 1536
Asn Asn Phe Arg Arg Ala Gln Ile Trp Gly Ala Lys Tyr Phe Lys Asn
500 505 510
aac ttc aac agg ctg gtc aag atc aag aca aaa gtt gat cca gaa aac 1584
Asn Phe Asn Arg Leu Val Lys Ile Lys Thr Lys Val Asp Pro Glu Asn
515 520 525
ttg ttc agg cac gaa caa tct att cca cca ttg cca att aga agg caa 1632
Leu Phe Arg His Glu Gln Ser Ile Pro Pro Leu Pro Ile Arg Arg Gln
530 535 540
acc tct 1638
Thr Ser
545
<210> 117
<211> 546
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 117
Met Arg Ile Ser Lys Pro Ile Pro Val Ile Ser Tyr Ile Leu Phe Leu
1 5 10 15
Ala Gln Tyr Phe Ser Leu His Thr Lys Glu Pro Thr Ser Ser Thr Ser
20 25 30
Leu Gln Asp Asp Phe Val Lys Cys Leu His Arg Asn Thr Asn Val Asp
35 40 45
Phe Pro Leu Asp Lys Thr Phe Phe Thr Pro Gln Arg Asn Ser Ser Asn
50 55 60
Phe Lys Glu Val Leu Asn Ser Thr Val Trp Asn Gln Arg Tyr Met Thr
65 70 75 80
Lys Ser Met Pro Lys Pro Glu Phe Ile Phe Lys Pro Val His Glu Ser
85 90 95
His Val Gln Ala Ser Ile Ile Cys Ser Lys Lys Leu Arg Ile His Phe
100 105 110
Arg Val Arg Ser Gly Gly His Asp Tyr Glu Gly Leu Ser Tyr Val Ser
115 120 125
Gln Met Glu Thr Pro Phe Val Leu Ile Asp Leu Trp Lys Leu Lys Gln
130 135 140
Ile Asn Val Asp Ile Glu Asp Asn Ser Ala Trp Val Gln Ala Gly Ala
145 150 155 160
Thr Thr Gly Glu Leu Tyr Tyr Arg Ile Ala Glu Lys Ser Lys Val His
165 170 175
Gly Phe Pro Ala Gly Leu Cys Ser Thr Val Gly Ile Gly Gly His Ile
180 185 190
Thr Gly Gly Gly Tyr Gly Thr Leu Met Arg Lys Tyr Gly Leu Ser Thr
195 200 205
Asp Asn Val Leu Asp Ala Arg Ile Val Asp Ala Asn Gly Gln Leu Leu
210 215 220
Asn Arg Thr Ala Met Gly Glu Asp Leu Phe Trp Ala Ile Ser Gly Gly
225 230 235 240
Gly Gly Gly Ser Phe Gly Val Ile Leu Ala Trp Lys Ile Lys Leu Val
245 250 255
Pro Val Pro Glu Thr Leu Thr Ile Phe Thr Ile Thr Lys Thr Leu Glu
260 265 270
Gln Asp Ala Gly Phe Lys Ile Leu Ser Lys Trp Gln Arg Ile Val Asp
275 280 285
Lys Leu Val Glu Glu Leu Ser Ile Arg Val Leu Phe Arg Asn Thr Gly
290 295 300
Asn Lys Thr Val Thr Thr Thr Tyr Arg Gly Glu Phe Leu Gly Glu Lys
305 310 315 320
Gly Thr Leu Met Glu Val Met Asn Lys Cys Phe Pro Glu Leu Gly Leu
325 330 335
Thr Gln Glu Asp Cys Ile Glu Met Ser Trp Ile Glu Thr Val Val Asn
340 345 350
Asn Ala Gly Phe Thr Thr Arg Pro Pro Pro Pro Leu Glu Ile Leu Leu
355 360 365
Gln Pro Ser Ser Pro Tyr Gly Arg Pro Tyr Phe Lys Ala Lys Ser Asp
370 375 380
Phe Val Lys Glu Pro Ile Pro Val Leu Gly Leu Lys Gly Met Phe Lys
385 390 395 400
Arg Met Ile Glu Glu Asp Ala Ser Phe Leu Ile Trp Lys Pro Tyr Gly
405 410 415
Gly Met Met Ala Arg Ile Pro Glu Ser Glu Leu Pro Phe Pro His Arg
420 425 430
Asn Gly Thr Ile Phe Lys Ile His Tyr Phe Thr Lys Trp Ser Glu Asn
435 440 445
Gly Ser Ser Ser Asp Asp Lys His Met Lys Trp Ile Arg Glu Leu Tyr
450 455 460
Ser Tyr Met Thr Pro Tyr Val Ser Ser Asn Pro Arg Gln Ala Tyr Val
465 470 475 480
Asn Tyr Arg Asp Leu Asp Ile Gly Gln Asn Lys Lys Asn Asp Ser Glu
485 490 495
Asn Asn Phe Arg Arg Ala Gln Ile Trp Gly Ala Lys Tyr Phe Lys Asn
500 505 510
Asn Phe Asn Arg Leu Val Lys Ile Lys Thr Lys Val Asp Pro Glu Asn
515 520 525
Leu Phe Arg His Glu Gln Ser Ile Pro Pro Leu Pro Ile Arg Arg Gln
530 535 540
Thr Ser
545
<210> 118
<211> 1569
<212> DNA
<213> 人工序列
<220>
<223> CBS146-A0A1J3J6A2-无信号肽
<220>
<221> CDS
<222> (1)..(1569)
<400> 118
atg aaa gaa cct acc tct tct acc tca ttg caa gat gat ttt gtt aag 48
Met Lys Glu Pro Thr Ser Ser Thr Ser Leu Gln Asp Asp Phe Val Lys
1 5 10 15
tgc ttg cac aga aac acc aac gtt gat ttt cca ttg gac aag act ttc 96
Cys Leu His Arg Asn Thr Asn Val Asp Phe Pro Leu Asp Lys Thr Phe
20 25 30
ttc acc cca caa aga aac tcc tcc aac ttc aaa gaa gtc ttg aac tct 144
Phe Thr Pro Gln Arg Asn Ser Ser Asn Phe Lys Glu Val Leu Asn Ser
35 40 45
acc gtc tgg aat caa aga tat atg acc aag tct atg cca aag cca gag 192
Thr Val Trp Asn Gln Arg Tyr Met Thr Lys Ser Met Pro Lys Pro Glu
50 55 60
ttc att ttt aag cca gtt cat gaa tcc cat gtc caa gcc tct att att 240
Phe Ile Phe Lys Pro Val His Glu Ser His Val Gln Ala Ser Ile Ile
65 70 75 80
tgc tct aag aag ttg aga atc cac ttc aga gtt aga tct ggt ggt cat 288
Cys Ser Lys Lys Leu Arg Ile His Phe Arg Val Arg Ser Gly Gly His
85 90 95
gat tat gag ggt ttg tct tac gtt tct caa atg gaa act cca ttc gtc 336
Asp Tyr Glu Gly Leu Ser Tyr Val Ser Gln Met Glu Thr Pro Phe Val
100 105 110
ttg atc gat ttg tgg aag ttg aag cag att aac gtc gac atc gaa gat 384
Leu Ile Asp Leu Trp Lys Leu Lys Gln Ile Asn Val Asp Ile Glu Asp
115 120 125
aat tct gct tgg gtt caa gct ggt gct act act ggt gag ttg tat tat 432
Asn Ser Ala Trp Val Gln Ala Gly Ala Thr Thr Gly Glu Leu Tyr Tyr
130 135 140
aga att gcc gag aag tct aag gtt cat ggt ttt cca gct ggt ttg tgt 480
Arg Ile Ala Glu Lys Ser Lys Val His Gly Phe Pro Ala Gly Leu Cys
145 150 155 160
tct act gtt ggt ata ggt ggt cat atc act ggt ggt ggt tat ggt act 528
Ser Thr Val Gly Ile Gly Gly His Ile Thr Gly Gly Gly Tyr Gly Thr
165 170 175
ttg atg aga aag tat ggt ttg tcc acc gat aac gtt ttg gat gct aga 576
Leu Met Arg Lys Tyr Gly Leu Ser Thr Asp Asn Val Leu Asp Ala Arg
180 185 190
atc gtt gat gct aat ggt cag ttg ttg aac aga act gct atg ggt gaa 624
Ile Val Asp Ala Asn Gly Gln Leu Leu Asn Arg Thr Ala Met Gly Glu
195 200 205
gat ttg ttc tgg gct att tct ggt ggc ggt ggt ggt agt ttt ggt gtt 672
Asp Leu Phe Trp Ala Ile Ser Gly Gly Gly Gly Gly Ser Phe Gly Val
210 215 220
att ttg gct tgg aag att aag ttg gtt cca gtt cca gaa acc ttg acc 720
Ile Leu Ala Trp Lys Ile Lys Leu Val Pro Val Pro Glu Thr Leu Thr
225 230 235 240
att ttc act att acc aag acc ttg gaa caa gat gcc ggt ttc aag att 768
Ile Phe Thr Ile Thr Lys Thr Leu Glu Gln Asp Ala Gly Phe Lys Ile
245 250 255
ttg tcc aaa tgg caa aga atc gtc gac aag ttg gtt gaa gaa ttg tcc 816
Leu Ser Lys Trp Gln Arg Ile Val Asp Lys Leu Val Glu Glu Leu Ser
260 265 270
atc aga gtc ttg ttc aga aac act ggt aac aag act gtt act act acc 864
Ile Arg Val Leu Phe Arg Asn Thr Gly Asn Lys Thr Val Thr Thr Thr
275 280 285
tac aga ggt gaa ttc ttg ggt gaa aaa ggt aca ttg atg gaa gtc atg 912
Tyr Arg Gly Glu Phe Leu Gly Glu Lys Gly Thr Leu Met Glu Val Met
290 295 300
aac aag tgc ttt cca gaa ttg ggt ttg act caa gag gat tgc att gaa 960
Asn Lys Cys Phe Pro Glu Leu Gly Leu Thr Gln Glu Asp Cys Ile Glu
305 310 315 320
atg tct tgg atc gaa act gtt gtc aac aat gct ggt ttt act act aga 1008
Met Ser Trp Ile Glu Thr Val Val Asn Asn Ala Gly Phe Thr Thr Arg
325 330 335
cca cca cct cca ttg gaa att ttg ttg caa cca tct tct cca tac ggt 1056
Pro Pro Pro Pro Leu Glu Ile Leu Leu Gln Pro Ser Ser Pro Tyr Gly
340 345 350
aga cca tac ttc aaa gct aag tct gat ttc gtc aaa gaa ccc att cca 1104
Arg Pro Tyr Phe Lys Ala Lys Ser Asp Phe Val Lys Glu Pro Ile Pro
355 360 365
gtt ttg ggt tta aag ggt atg ttc aag aga atg atc gaa gag gat gcc 1152
Val Leu Gly Leu Lys Gly Met Phe Lys Arg Met Ile Glu Glu Asp Ala
370 375 380
tct ttc ttg att tgg aaa cca tac ggt ggt atg atg gct aga att cca 1200
Ser Phe Leu Ile Trp Lys Pro Tyr Gly Gly Met Met Ala Arg Ile Pro
385 390 395 400
gaa tct gaa ttg cca ttt cca cac aga aac ggt act att ttc aag atc 1248
Glu Ser Glu Leu Pro Phe Pro His Arg Asn Gly Thr Ile Phe Lys Ile
405 410 415
cat tac ttc acc aag tgg tcc gaa aat ggt tct tct tct gat gac aaa 1296
His Tyr Phe Thr Lys Trp Ser Glu Asn Gly Ser Ser Ser Asp Asp Lys
420 425 430
cac atg aag tgg atc agg gaa ctg tat tct tat atg acc cca tac gtt 1344
His Met Lys Trp Ile Arg Glu Leu Tyr Ser Tyr Met Thr Pro Tyr Val
435 440 445
tct tct aac cca aga caa gct tac gtt aac tac aga gat ttg gac atc 1392
Ser Ser Asn Pro Arg Gln Ala Tyr Val Asn Tyr Arg Asp Leu Asp Ile
450 455 460
ggt cag aac aag aag aac gat tct gaa aac aat ttc aga agg gct caa 1440
Gly Gln Asn Lys Lys Asn Asp Ser Glu Asn Asn Phe Arg Arg Ala Gln
465 470 475 480
att tgg ggt gct aag tac ttt aag aac aac ttc aac agg ctg gtc aag 1488
Ile Trp Gly Ala Lys Tyr Phe Lys Asn Asn Phe Asn Arg Leu Val Lys
485 490 495
atc aag aca aaa gtt gat cca gaa aac ttg ttc agg cac gaa caa tct 1536
Ile Lys Thr Lys Val Asp Pro Glu Asn Leu Phe Arg His Glu Gln Ser
500 505 510
att cca cca ttg cca att aga agg caa acc tct 1569
Ile Pro Pro Leu Pro Ile Arg Arg Gln Thr Ser
515 520
<210> 119
<211> 523
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 119
Met Lys Glu Pro Thr Ser Ser Thr Ser Leu Gln Asp Asp Phe Val Lys
1 5 10 15
Cys Leu His Arg Asn Thr Asn Val Asp Phe Pro Leu Asp Lys Thr Phe
20 25 30
Phe Thr Pro Gln Arg Asn Ser Ser Asn Phe Lys Glu Val Leu Asn Ser
35 40 45
Thr Val Trp Asn Gln Arg Tyr Met Thr Lys Ser Met Pro Lys Pro Glu
50 55 60
Phe Ile Phe Lys Pro Val His Glu Ser His Val Gln Ala Ser Ile Ile
65 70 75 80
Cys Ser Lys Lys Leu Arg Ile His Phe Arg Val Arg Ser Gly Gly His
85 90 95
Asp Tyr Glu Gly Leu Ser Tyr Val Ser Gln Met Glu Thr Pro Phe Val
100 105 110
Leu Ile Asp Leu Trp Lys Leu Lys Gln Ile Asn Val Asp Ile Glu Asp
115 120 125
Asn Ser Ala Trp Val Gln Ala Gly Ala Thr Thr Gly Glu Leu Tyr Tyr
130 135 140
Arg Ile Ala Glu Lys Ser Lys Val His Gly Phe Pro Ala Gly Leu Cys
145 150 155 160
Ser Thr Val Gly Ile Gly Gly His Ile Thr Gly Gly Gly Tyr Gly Thr
165 170 175
Leu Met Arg Lys Tyr Gly Leu Ser Thr Asp Asn Val Leu Asp Ala Arg
180 185 190
Ile Val Asp Ala Asn Gly Gln Leu Leu Asn Arg Thr Ala Met Gly Glu
195 200 205
Asp Leu Phe Trp Ala Ile Ser Gly Gly Gly Gly Gly Ser Phe Gly Val
210 215 220
Ile Leu Ala Trp Lys Ile Lys Leu Val Pro Val Pro Glu Thr Leu Thr
225 230 235 240
Ile Phe Thr Ile Thr Lys Thr Leu Glu Gln Asp Ala Gly Phe Lys Ile
245 250 255
Leu Ser Lys Trp Gln Arg Ile Val Asp Lys Leu Val Glu Glu Leu Ser
260 265 270
Ile Arg Val Leu Phe Arg Asn Thr Gly Asn Lys Thr Val Thr Thr Thr
275 280 285
Tyr Arg Gly Glu Phe Leu Gly Glu Lys Gly Thr Leu Met Glu Val Met
290 295 300
Asn Lys Cys Phe Pro Glu Leu Gly Leu Thr Gln Glu Asp Cys Ile Glu
305 310 315 320
Met Ser Trp Ile Glu Thr Val Val Asn Asn Ala Gly Phe Thr Thr Arg
325 330 335
Pro Pro Pro Pro Leu Glu Ile Leu Leu Gln Pro Ser Ser Pro Tyr Gly
340 345 350
Arg Pro Tyr Phe Lys Ala Lys Ser Asp Phe Val Lys Glu Pro Ile Pro
355 360 365
Val Leu Gly Leu Lys Gly Met Phe Lys Arg Met Ile Glu Glu Asp Ala
370 375 380
Ser Phe Leu Ile Trp Lys Pro Tyr Gly Gly Met Met Ala Arg Ile Pro
385 390 395 400
Glu Ser Glu Leu Pro Phe Pro His Arg Asn Gly Thr Ile Phe Lys Ile
405 410 415
His Tyr Phe Thr Lys Trp Ser Glu Asn Gly Ser Ser Ser Asp Asp Lys
420 425 430
His Met Lys Trp Ile Arg Glu Leu Tyr Ser Tyr Met Thr Pro Tyr Val
435 440 445
Ser Ser Asn Pro Arg Gln Ala Tyr Val Asn Tyr Arg Asp Leu Asp Ile
450 455 460
Gly Gln Asn Lys Lys Asn Asp Ser Glu Asn Asn Phe Arg Arg Ala Gln
465 470 475 480
Ile Trp Gly Ala Lys Tyr Phe Lys Asn Asn Phe Asn Arg Leu Val Lys
485 490 495
Ile Lys Thr Lys Val Asp Pro Glu Asn Leu Phe Arg His Glu Gln Ser
500 505 510
Ile Pro Pro Leu Pro Ile Arg Arg Gln Thr Ser
515 520
<210> 120
<211> 1593
<212> DNA
<213> 人工序列
<220>
<223> CBS146-A0A1J3J6A2-无信号肽-strep标签
<220>
<221> CDS
<222> (1)..(1593)
<400> 120
atg aaa gaa cct acc tct tct acc tca ttg caa gat gat ttt gtt aag 48
Met Lys Glu Pro Thr Ser Ser Thr Ser Leu Gln Asp Asp Phe Val Lys
1 5 10 15
tgc ttg cac aga aac acc aac gtt gat ttt cca ttg gac aag act ttc 96
Cys Leu His Arg Asn Thr Asn Val Asp Phe Pro Leu Asp Lys Thr Phe
20 25 30
ttc acc cca caa aga aac tcc tcc aac ttc aaa gaa gtc ttg aac tct 144
Phe Thr Pro Gln Arg Asn Ser Ser Asn Phe Lys Glu Val Leu Asn Ser
35 40 45
acc gtc tgg aat caa aga tat atg acc aag tct atg cca aag cca gag 192
Thr Val Trp Asn Gln Arg Tyr Met Thr Lys Ser Met Pro Lys Pro Glu
50 55 60
ttc att ttt aag cca gtt cat gaa tcc cat gtc caa gcc tct att att 240
Phe Ile Phe Lys Pro Val His Glu Ser His Val Gln Ala Ser Ile Ile
65 70 75 80
tgc tct aag aag ttg aga atc cac ttc aga gtt aga tct ggt ggt cat 288
Cys Ser Lys Lys Leu Arg Ile His Phe Arg Val Arg Ser Gly Gly His
85 90 95
gat tat gag ggt ttg tct tac gtt tct caa atg gaa act cca ttc gtc 336
Asp Tyr Glu Gly Leu Ser Tyr Val Ser Gln Met Glu Thr Pro Phe Val
100 105 110
ttg atc gat ttg tgg aag ttg aag cag att aac gtc gac atc gaa gat 384
Leu Ile Asp Leu Trp Lys Leu Lys Gln Ile Asn Val Asp Ile Glu Asp
115 120 125
aat tct gct tgg gtt caa gct ggt gct act act ggt gag ttg tat tat 432
Asn Ser Ala Trp Val Gln Ala Gly Ala Thr Thr Gly Glu Leu Tyr Tyr
130 135 140
aga att gcc gag aag tct aag gtt cat ggt ttt cca gct ggt ttg tgt 480
Arg Ile Ala Glu Lys Ser Lys Val His Gly Phe Pro Ala Gly Leu Cys
145 150 155 160
tct act gtt ggt ata ggt ggt cat atc act ggt ggt ggt tat ggt act 528
Ser Thr Val Gly Ile Gly Gly His Ile Thr Gly Gly Gly Tyr Gly Thr
165 170 175
ttg atg aga aag tat ggt ttg tcc acc gat aac gtt ttg gat gct aga 576
Leu Met Arg Lys Tyr Gly Leu Ser Thr Asp Asn Val Leu Asp Ala Arg
180 185 190
atc gtt gat gct aat ggt cag ttg ttg aac aga act gct atg ggt gaa 624
Ile Val Asp Ala Asn Gly Gln Leu Leu Asn Arg Thr Ala Met Gly Glu
195 200 205
gat ttg ttc tgg gct att tct ggt ggc ggt ggt ggt agt ttt ggt gtt 672
Asp Leu Phe Trp Ala Ile Ser Gly Gly Gly Gly Gly Ser Phe Gly Val
210 215 220
att ttg gct tgg aag att aag ttg gtt cca gtt cca gaa acc ttg acc 720
Ile Leu Ala Trp Lys Ile Lys Leu Val Pro Val Pro Glu Thr Leu Thr
225 230 235 240
att ttc act att acc aag acc ttg gaa caa gat gcc ggt ttc aag att 768
Ile Phe Thr Ile Thr Lys Thr Leu Glu Gln Asp Ala Gly Phe Lys Ile
245 250 255
ttg tcc aaa tgg caa aga atc gtc gac aag ttg gtt gaa gaa ttg tcc 816
Leu Ser Lys Trp Gln Arg Ile Val Asp Lys Leu Val Glu Glu Leu Ser
260 265 270
atc aga gtc ttg ttc aga aac act ggt aac aag act gtt act act acc 864
Ile Arg Val Leu Phe Arg Asn Thr Gly Asn Lys Thr Val Thr Thr Thr
275 280 285
tac aga ggt gaa ttc ttg ggt gaa aaa ggt aca ttg atg gaa gtc atg 912
Tyr Arg Gly Glu Phe Leu Gly Glu Lys Gly Thr Leu Met Glu Val Met
290 295 300
aac aag tgc ttt cca gaa ttg ggt ttg act caa gag gat tgc att gaa 960
Asn Lys Cys Phe Pro Glu Leu Gly Leu Thr Gln Glu Asp Cys Ile Glu
305 310 315 320
atg tct tgg atc gaa act gtt gtc aac aat gct ggt ttt act act aga 1008
Met Ser Trp Ile Glu Thr Val Val Asn Asn Ala Gly Phe Thr Thr Arg
325 330 335
cca cca cct cca ttg gaa att ttg ttg caa cca tct tct cca tac ggt 1056
Pro Pro Pro Pro Leu Glu Ile Leu Leu Gln Pro Ser Ser Pro Tyr Gly
340 345 350
aga cca tac ttc aaa gct aag tct gat ttc gtc aaa gaa ccc att cca 1104
Arg Pro Tyr Phe Lys Ala Lys Ser Asp Phe Val Lys Glu Pro Ile Pro
355 360 365
gtt ttg ggt tta aag ggt atg ttc aag aga atg atc gaa gag gat gcc 1152
Val Leu Gly Leu Lys Gly Met Phe Lys Arg Met Ile Glu Glu Asp Ala
370 375 380
tct ttc ttg att tgg aaa cca tac ggt ggt atg atg gct aga att cca 1200
Ser Phe Leu Ile Trp Lys Pro Tyr Gly Gly Met Met Ala Arg Ile Pro
385 390 395 400
gaa tct gaa ttg cca ttt cca cac aga aac ggt act att ttc aag atc 1248
Glu Ser Glu Leu Pro Phe Pro His Arg Asn Gly Thr Ile Phe Lys Ile
405 410 415
cat tac ttc acc aag tgg tcc gaa aat ggt tct tct tct gat gac aaa 1296
His Tyr Phe Thr Lys Trp Ser Glu Asn Gly Ser Ser Ser Asp Asp Lys
420 425 430
cac atg aag tgg atc agg gaa ctg tat tct tat atg acc cca tac gtt 1344
His Met Lys Trp Ile Arg Glu Leu Tyr Ser Tyr Met Thr Pro Tyr Val
435 440 445
tct tct aac cca aga caa gct tac gtt aac tac aga gat ttg gac atc 1392
Ser Ser Asn Pro Arg Gln Ala Tyr Val Asn Tyr Arg Asp Leu Asp Ile
450 455 460
ggt cag aac aag aag aac gat tct gaa aac aat ttc aga agg gct caa 1440
Gly Gln Asn Lys Lys Asn Asp Ser Glu Asn Asn Phe Arg Arg Ala Gln
465 470 475 480
att tgg ggt gct aag tac ttt aag aac aac ttc aac agg ctg gtc aag 1488
Ile Trp Gly Ala Lys Tyr Phe Lys Asn Asn Phe Asn Arg Leu Val Lys
485 490 495
atc aag aca aaa gtt gat cca gaa aac ttg ttc agg cac gaa caa tct 1536
Ile Lys Thr Lys Val Asp Pro Glu Asn Leu Phe Arg His Glu Gln Ser
500 505 510
att cca cca ttg cca att aga agg caa acc tct tgg tca cat cca caa 1584
Ile Pro Pro Leu Pro Ile Arg Arg Gln Thr Ser Trp Ser His Pro Gln
515 520 525
ttt gaa aag 1593
Phe Glu Lys
530
<210> 121
<211> 531
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 121
Met Lys Glu Pro Thr Ser Ser Thr Ser Leu Gln Asp Asp Phe Val Lys
1 5 10 15
Cys Leu His Arg Asn Thr Asn Val Asp Phe Pro Leu Asp Lys Thr Phe
20 25 30
Phe Thr Pro Gln Arg Asn Ser Ser Asn Phe Lys Glu Val Leu Asn Ser
35 40 45
Thr Val Trp Asn Gln Arg Tyr Met Thr Lys Ser Met Pro Lys Pro Glu
50 55 60
Phe Ile Phe Lys Pro Val His Glu Ser His Val Gln Ala Ser Ile Ile
65 70 75 80
Cys Ser Lys Lys Leu Arg Ile His Phe Arg Val Arg Ser Gly Gly His
85 90 95
Asp Tyr Glu Gly Leu Ser Tyr Val Ser Gln Met Glu Thr Pro Phe Val
100 105 110
Leu Ile Asp Leu Trp Lys Leu Lys Gln Ile Asn Val Asp Ile Glu Asp
115 120 125
Asn Ser Ala Trp Val Gln Ala Gly Ala Thr Thr Gly Glu Leu Tyr Tyr
130 135 140
Arg Ile Ala Glu Lys Ser Lys Val His Gly Phe Pro Ala Gly Leu Cys
145 150 155 160
Ser Thr Val Gly Ile Gly Gly His Ile Thr Gly Gly Gly Tyr Gly Thr
165 170 175
Leu Met Arg Lys Tyr Gly Leu Ser Thr Asp Asn Val Leu Asp Ala Arg
180 185 190
Ile Val Asp Ala Asn Gly Gln Leu Leu Asn Arg Thr Ala Met Gly Glu
195 200 205
Asp Leu Phe Trp Ala Ile Ser Gly Gly Gly Gly Gly Ser Phe Gly Val
210 215 220
Ile Leu Ala Trp Lys Ile Lys Leu Val Pro Val Pro Glu Thr Leu Thr
225 230 235 240
Ile Phe Thr Ile Thr Lys Thr Leu Glu Gln Asp Ala Gly Phe Lys Ile
245 250 255
Leu Ser Lys Trp Gln Arg Ile Val Asp Lys Leu Val Glu Glu Leu Ser
260 265 270
Ile Arg Val Leu Phe Arg Asn Thr Gly Asn Lys Thr Val Thr Thr Thr
275 280 285
Tyr Arg Gly Glu Phe Leu Gly Glu Lys Gly Thr Leu Met Glu Val Met
290 295 300
Asn Lys Cys Phe Pro Glu Leu Gly Leu Thr Gln Glu Asp Cys Ile Glu
305 310 315 320
Met Ser Trp Ile Glu Thr Val Val Asn Asn Ala Gly Phe Thr Thr Arg
325 330 335
Pro Pro Pro Pro Leu Glu Ile Leu Leu Gln Pro Ser Ser Pro Tyr Gly
340 345 350
Arg Pro Tyr Phe Lys Ala Lys Ser Asp Phe Val Lys Glu Pro Ile Pro
355 360 365
Val Leu Gly Leu Lys Gly Met Phe Lys Arg Met Ile Glu Glu Asp Ala
370 375 380
Ser Phe Leu Ile Trp Lys Pro Tyr Gly Gly Met Met Ala Arg Ile Pro
385 390 395 400
Glu Ser Glu Leu Pro Phe Pro His Arg Asn Gly Thr Ile Phe Lys Ile
405 410 415
His Tyr Phe Thr Lys Trp Ser Glu Asn Gly Ser Ser Ser Asp Asp Lys
420 425 430
His Met Lys Trp Ile Arg Glu Leu Tyr Ser Tyr Met Thr Pro Tyr Val
435 440 445
Ser Ser Asn Pro Arg Gln Ala Tyr Val Asn Tyr Arg Asp Leu Asp Ile
450 455 460
Gly Gln Asn Lys Lys Asn Asp Ser Glu Asn Asn Phe Arg Arg Ala Gln
465 470 475 480
Ile Trp Gly Ala Lys Tyr Phe Lys Asn Asn Phe Asn Arg Leu Val Lys
485 490 495
Ile Lys Thr Lys Val Asp Pro Glu Asn Leu Phe Arg His Glu Gln Ser
500 505 510
Ile Pro Pro Leu Pro Ile Arg Arg Gln Thr Ser Trp Ser His Pro Gln
515 520 525
Phe Glu Lys
530
<210> 122
<211> 1650
<212> DNA
<213> 人工序列
<220>
<223> CBS146-A0A1J3J6A2-截断alpha因子-无信号肽-strep标签
<220>
<221> CDS
<222> (1)..(1650)
<400> 122
atg aga ttt cct tcc atc ttc acg gct gtg cta ttt gca gca tcc tcc 48
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser
1 5 10 15
gca ctt gca atg aaa gaa cct acc tct tct acc tca ttg caa gat gat 96
Ala Leu Ala Met Lys Glu Pro Thr Ser Ser Thr Ser Leu Gln Asp Asp
20 25 30
ttt gtt aag tgc ttg cac aga aac acc aac gtt gat ttt cca ttg gac 144
Phe Val Lys Cys Leu His Arg Asn Thr Asn Val Asp Phe Pro Leu Asp
35 40 45
aag act ttc ttc acc cca caa aga aac tcc tcc aac ttc aaa gaa gtc 192
Lys Thr Phe Phe Thr Pro Gln Arg Asn Ser Ser Asn Phe Lys Glu Val
50 55 60
ttg aac tct acc gtc tgg aat caa aga tat atg acc aag tct atg cca 240
Leu Asn Ser Thr Val Trp Asn Gln Arg Tyr Met Thr Lys Ser Met Pro
65 70 75 80
aag cca gag ttc att ttt aag cca gtt cat gaa tcc cat gtc caa gcc 288
Lys Pro Glu Phe Ile Phe Lys Pro Val His Glu Ser His Val Gln Ala
85 90 95
tct att att tgc tct aag aag ttg aga atc cac ttc aga gtt aga tct 336
Ser Ile Ile Cys Ser Lys Lys Leu Arg Ile His Phe Arg Val Arg Ser
100 105 110
ggt ggt cat gat tat gag ggt ttg tct tac gtt tct caa atg gaa act 384
Gly Gly His Asp Tyr Glu Gly Leu Ser Tyr Val Ser Gln Met Glu Thr
115 120 125
cca ttc gtc ttg atc gat ttg tgg aag ttg aag cag att aac gtc gac 432
Pro Phe Val Leu Ile Asp Leu Trp Lys Leu Lys Gln Ile Asn Val Asp
130 135 140
atc gaa gat aat tct gct tgg gtt caa gct ggt gct act act ggt gag 480
Ile Glu Asp Asn Ser Ala Trp Val Gln Ala Gly Ala Thr Thr Gly Glu
145 150 155 160
ttg tat tat aga att gcc gag aag tct aag gtt cat ggt ttt cca gct 528
Leu Tyr Tyr Arg Ile Ala Glu Lys Ser Lys Val His Gly Phe Pro Ala
165 170 175
ggt ttg tgt tct act gtt ggt ata ggt ggt cat atc act ggt ggt ggt 576
Gly Leu Cys Ser Thr Val Gly Ile Gly Gly His Ile Thr Gly Gly Gly
180 185 190
tat ggt act ttg atg aga aag tat ggt ttg tcc acc gat aac gtt ttg 624
Tyr Gly Thr Leu Met Arg Lys Tyr Gly Leu Ser Thr Asp Asn Val Leu
195 200 205
gat gct aga atc gtt gat gct aat ggt cag ttg ttg aac aga act gct 672
Asp Ala Arg Ile Val Asp Ala Asn Gly Gln Leu Leu Asn Arg Thr Ala
210 215 220
atg ggt gaa gat ttg ttc tgg gct att tct ggt ggc ggt ggt ggt agt 720
Met Gly Glu Asp Leu Phe Trp Ala Ile Ser Gly Gly Gly Gly Gly Ser
225 230 235 240
ttt ggt gtt att ttg gct tgg aag att aag ttg gtt cca gtt cca gaa 768
Phe Gly Val Ile Leu Ala Trp Lys Ile Lys Leu Val Pro Val Pro Glu
245 250 255
acc ttg acc att ttc act att acc aag acc ttg gaa caa gat gcc ggt 816
Thr Leu Thr Ile Phe Thr Ile Thr Lys Thr Leu Glu Gln Asp Ala Gly
260 265 270
ttc aag att ttg tcc aaa tgg caa aga atc gtc gac aag ttg gtt gaa 864
Phe Lys Ile Leu Ser Lys Trp Gln Arg Ile Val Asp Lys Leu Val Glu
275 280 285
gaa ttg tcc atc aga gtc ttg ttc aga aac act ggt aac aag act gtt 912
Glu Leu Ser Ile Arg Val Leu Phe Arg Asn Thr Gly Asn Lys Thr Val
290 295 300
act act acc tac aga ggt gaa ttc ttg ggt gaa aaa ggt aca ttg atg 960
Thr Thr Thr Tyr Arg Gly Glu Phe Leu Gly Glu Lys Gly Thr Leu Met
305 310 315 320
gaa gtc atg aac aag tgc ttt cca gaa ttg ggt ttg act caa gag gat 1008
Glu Val Met Asn Lys Cys Phe Pro Glu Leu Gly Leu Thr Gln Glu Asp
325 330 335
tgc att gaa atg tct tgg atc gaa act gtt gtc aac aat gct ggt ttt 1056
Cys Ile Glu Met Ser Trp Ile Glu Thr Val Val Asn Asn Ala Gly Phe
340 345 350
act act aga cca cca cct cca ttg gaa att ttg ttg caa cca tct tct 1104
Thr Thr Arg Pro Pro Pro Pro Leu Glu Ile Leu Leu Gln Pro Ser Ser
355 360 365
cca tac ggt aga cca tac ttc aaa gct aag tct gat ttc gtc aaa gaa 1152
Pro Tyr Gly Arg Pro Tyr Phe Lys Ala Lys Ser Asp Phe Val Lys Glu
370 375 380
ccc att cca gtt ttg ggt tta aag ggt atg ttc aag aga atg atc gaa 1200
Pro Ile Pro Val Leu Gly Leu Lys Gly Met Phe Lys Arg Met Ile Glu
385 390 395 400
gag gat gcc tct ttc ttg att tgg aaa cca tac ggt ggt atg atg gct 1248
Glu Asp Ala Ser Phe Leu Ile Trp Lys Pro Tyr Gly Gly Met Met Ala
405 410 415
aga att cca gaa tct gaa ttg cca ttt cca cac aga aac ggt act att 1296
Arg Ile Pro Glu Ser Glu Leu Pro Phe Pro His Arg Asn Gly Thr Ile
420 425 430
ttc aag atc cat tac ttc acc aag tgg tcc gaa aat ggt tct tct tct 1344
Phe Lys Ile His Tyr Phe Thr Lys Trp Ser Glu Asn Gly Ser Ser Ser
435 440 445
gat gac aaa cac atg aag tgg atc agg gaa ctg tat tct tat atg acc 1392
Asp Asp Lys His Met Lys Trp Ile Arg Glu Leu Tyr Ser Tyr Met Thr
450 455 460
cca tac gtt tct tct aac cca aga caa gct tac gtt aac tac aga gat 1440
Pro Tyr Val Ser Ser Asn Pro Arg Gln Ala Tyr Val Asn Tyr Arg Asp
465 470 475 480
ttg gac atc ggt cag aac aag aag aac gat tct gaa aac aat ttc aga 1488
Leu Asp Ile Gly Gln Asn Lys Lys Asn Asp Ser Glu Asn Asn Phe Arg
485 490 495
agg gct caa att tgg ggt gct aag tac ttt aag aac aac ttc aac agg 1536
Arg Ala Gln Ile Trp Gly Ala Lys Tyr Phe Lys Asn Asn Phe Asn Arg
500 505 510
ctg gtc aag atc aag aca aaa gtt gat cca gaa aac ttg ttc agg cac 1584
Leu Val Lys Ile Lys Thr Lys Val Asp Pro Glu Asn Leu Phe Arg His
515 520 525
gaa caa tct att cca cca ttg cca att aga agg caa acc tct tgg tca 1632
Glu Gln Ser Ile Pro Pro Leu Pro Ile Arg Arg Gln Thr Ser Trp Ser
530 535 540
cat cca caa ttt gaa aag 1650
His Pro Gln Phe Glu Lys
545 550
<210> 123
<211> 550
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 123
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser
1 5 10 15
Ala Leu Ala Met Lys Glu Pro Thr Ser Ser Thr Ser Leu Gln Asp Asp
20 25 30
Phe Val Lys Cys Leu His Arg Asn Thr Asn Val Asp Phe Pro Leu Asp
35 40 45
Lys Thr Phe Phe Thr Pro Gln Arg Asn Ser Ser Asn Phe Lys Glu Val
50 55 60
Leu Asn Ser Thr Val Trp Asn Gln Arg Tyr Met Thr Lys Ser Met Pro
65 70 75 80
Lys Pro Glu Phe Ile Phe Lys Pro Val His Glu Ser His Val Gln Ala
85 90 95
Ser Ile Ile Cys Ser Lys Lys Leu Arg Ile His Phe Arg Val Arg Ser
100 105 110
Gly Gly His Asp Tyr Glu Gly Leu Ser Tyr Val Ser Gln Met Glu Thr
115 120 125
Pro Phe Val Leu Ile Asp Leu Trp Lys Leu Lys Gln Ile Asn Val Asp
130 135 140
Ile Glu Asp Asn Ser Ala Trp Val Gln Ala Gly Ala Thr Thr Gly Glu
145 150 155 160
Leu Tyr Tyr Arg Ile Ala Glu Lys Ser Lys Val His Gly Phe Pro Ala
165 170 175
Gly Leu Cys Ser Thr Val Gly Ile Gly Gly His Ile Thr Gly Gly Gly
180 185 190
Tyr Gly Thr Leu Met Arg Lys Tyr Gly Leu Ser Thr Asp Asn Val Leu
195 200 205
Asp Ala Arg Ile Val Asp Ala Asn Gly Gln Leu Leu Asn Arg Thr Ala
210 215 220
Met Gly Glu Asp Leu Phe Trp Ala Ile Ser Gly Gly Gly Gly Gly Ser
225 230 235 240
Phe Gly Val Ile Leu Ala Trp Lys Ile Lys Leu Val Pro Val Pro Glu
245 250 255
Thr Leu Thr Ile Phe Thr Ile Thr Lys Thr Leu Glu Gln Asp Ala Gly
260 265 270
Phe Lys Ile Leu Ser Lys Trp Gln Arg Ile Val Asp Lys Leu Val Glu
275 280 285
Glu Leu Ser Ile Arg Val Leu Phe Arg Asn Thr Gly Asn Lys Thr Val
290 295 300
Thr Thr Thr Tyr Arg Gly Glu Phe Leu Gly Glu Lys Gly Thr Leu Met
305 310 315 320
Glu Val Met Asn Lys Cys Phe Pro Glu Leu Gly Leu Thr Gln Glu Asp
325 330 335
Cys Ile Glu Met Ser Trp Ile Glu Thr Val Val Asn Asn Ala Gly Phe
340 345 350
Thr Thr Arg Pro Pro Pro Pro Leu Glu Ile Leu Leu Gln Pro Ser Ser
355 360 365
Pro Tyr Gly Arg Pro Tyr Phe Lys Ala Lys Ser Asp Phe Val Lys Glu
370 375 380
Pro Ile Pro Val Leu Gly Leu Lys Gly Met Phe Lys Arg Met Ile Glu
385 390 395 400
Glu Asp Ala Ser Phe Leu Ile Trp Lys Pro Tyr Gly Gly Met Met Ala
405 410 415
Arg Ile Pro Glu Ser Glu Leu Pro Phe Pro His Arg Asn Gly Thr Ile
420 425 430
Phe Lys Ile His Tyr Phe Thr Lys Trp Ser Glu Asn Gly Ser Ser Ser
435 440 445
Asp Asp Lys His Met Lys Trp Ile Arg Glu Leu Tyr Ser Tyr Met Thr
450 455 460
Pro Tyr Val Ser Ser Asn Pro Arg Gln Ala Tyr Val Asn Tyr Arg Asp
465 470 475 480
Leu Asp Ile Gly Gln Asn Lys Lys Asn Asp Ser Glu Asn Asn Phe Arg
485 490 495
Arg Ala Gln Ile Trp Gly Ala Lys Tyr Phe Lys Asn Asn Phe Asn Arg
500 505 510
Leu Val Lys Ile Lys Thr Lys Val Asp Pro Glu Asn Leu Phe Arg His
515 520 525
Glu Gln Ser Ile Pro Pro Leu Pro Ile Arg Arg Gln Thr Ser Trp Ser
530 535 540
His Pro Gln Phe Glu Lys
545 550
<210> 124
<211> 1860
<212> DNA
<213> 人工序列
<220>
<223> CBS146-A0A1J3J6A2-全长alpha因子-无信号肽-strep标签
<220>
<221> CDS
<222> (1)..(1860)
<400> 124
atg aga ttt cct tca att ttt act gct gtt tta ttc gca gca tcc tcc 48
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser
1 5 10 15
gca tta gct gct cca gtc aac act aca aca gaa gat gaa acg gca caa 96
Ala Leu Ala Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln
20 25 30
att ccg gct gaa gct gtc atc ggt tac tca gat tta gaa ggg gat ttc 144
Ile Pro Ala Glu Ala Val Ile Gly Tyr Ser Asp Leu Glu Gly Asp Phe
35 40 45
gat gtt gct gtt ttg cca ttt tcc aac agc aca aat aac ggg tta ttg 192
Asp Val Ala Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu
50 55 60
ttt ata aat act act att gcc agc att gct gct aaa gaa gaa ggg gta 240
Phe Ile Asn Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val
65 70 75 80
tct ctc gag aaa aga gag gct gaa gct atg aaa gaa cct acc tct tct 288
Ser Leu Glu Lys Arg Glu Ala Glu Ala Met Lys Glu Pro Thr Ser Ser
85 90 95
acc tca ttg caa gat gat ttt gtt aag tgc ttg cac aga aac acc aac 336
Thr Ser Leu Gln Asp Asp Phe Val Lys Cys Leu His Arg Asn Thr Asn
100 105 110
gtt gat ttt cca ttg gac aag act ttc ttc acc cca caa aga aac tcc 384
Val Asp Phe Pro Leu Asp Lys Thr Phe Phe Thr Pro Gln Arg Asn Ser
115 120 125
tcc aac ttc aaa gaa gtc ttg aac tct acc gtc tgg aat caa aga tat 432
Ser Asn Phe Lys Glu Val Leu Asn Ser Thr Val Trp Asn Gln Arg Tyr
130 135 140
atg acc aag tct atg cca aag cca gag ttc att ttt aag cca gtt cat 480
Met Thr Lys Ser Met Pro Lys Pro Glu Phe Ile Phe Lys Pro Val His
145 150 155 160
gaa tcc cat gtc caa gcc tct att att tgc tct aag aag ttg aga atc 528
Glu Ser His Val Gln Ala Ser Ile Ile Cys Ser Lys Lys Leu Arg Ile
165 170 175
cac ttc aga gtt aga tct ggt ggt cat gat tat gag ggt ttg tct tac 576
His Phe Arg Val Arg Ser Gly Gly His Asp Tyr Glu Gly Leu Ser Tyr
180 185 190
gtt tct caa atg gaa act cca ttc gtc ttg atc gat ttg tgg aag ttg 624
Val Ser Gln Met Glu Thr Pro Phe Val Leu Ile Asp Leu Trp Lys Leu
195 200 205
aag cag att aac gtc gac atc gaa gat aat tct gct tgg gtt caa gct 672
Lys Gln Ile Asn Val Asp Ile Glu Asp Asn Ser Ala Trp Val Gln Ala
210 215 220
ggt gct act act ggt gag ttg tat tat aga att gcc gag aag tct aag 720
Gly Ala Thr Thr Gly Glu Leu Tyr Tyr Arg Ile Ala Glu Lys Ser Lys
225 230 235 240
gtt cat ggt ttt cca gct ggt ttg tgt tct act gtt ggt ata ggt ggt 768
Val His Gly Phe Pro Ala Gly Leu Cys Ser Thr Val Gly Ile Gly Gly
245 250 255
cat atc act ggt ggt ggt tat ggt act ttg atg aga aag tat ggt ttg 816
His Ile Thr Gly Gly Gly Tyr Gly Thr Leu Met Arg Lys Tyr Gly Leu
260 265 270
tcc acc gat aac gtt ttg gat gct aga atc gtt gat gct aat ggt cag 864
Ser Thr Asp Asn Val Leu Asp Ala Arg Ile Val Asp Ala Asn Gly Gln
275 280 285
ttg ttg aac aga act gct atg ggt gaa gat ttg ttc tgg gct att tct 912
Leu Leu Asn Arg Thr Ala Met Gly Glu Asp Leu Phe Trp Ala Ile Ser
290 295 300
ggt ggc ggt ggt ggt agt ttt ggt gtt att ttg gct tgg aag att aag 960
Gly Gly Gly Gly Gly Ser Phe Gly Val Ile Leu Ala Trp Lys Ile Lys
305 310 315 320
ttg gtt cca gtt cca gaa acc ttg acc att ttc act att acc aag acc 1008
Leu Val Pro Val Pro Glu Thr Leu Thr Ile Phe Thr Ile Thr Lys Thr
325 330 335
ttg gaa caa gat gcc ggt ttc aag att ttg tcc aaa tgg caa aga atc 1056
Leu Glu Gln Asp Ala Gly Phe Lys Ile Leu Ser Lys Trp Gln Arg Ile
340 345 350
gtc gac aag ttg gtt gaa gaa ttg tcc atc aga gtc ttg ttc aga aac 1104
Val Asp Lys Leu Val Glu Glu Leu Ser Ile Arg Val Leu Phe Arg Asn
355 360 365
act ggt aac aag act gtt act act acc tac aga ggt gaa ttc ttg ggt 1152
Thr Gly Asn Lys Thr Val Thr Thr Thr Tyr Arg Gly Glu Phe Leu Gly
370 375 380
gaa aaa ggt aca ttg atg gaa gtc atg aac aag tgc ttt cca gaa ttg 1200
Glu Lys Gly Thr Leu Met Glu Val Met Asn Lys Cys Phe Pro Glu Leu
385 390 395 400
ggt ttg act caa gag gat tgc att gaa atg tct tgg atc gaa act gtt 1248
Gly Leu Thr Gln Glu Asp Cys Ile Glu Met Ser Trp Ile Glu Thr Val
405 410 415
gtc aac aat gct ggt ttt act act aga cca cca cct cca ttg gaa att 1296
Val Asn Asn Ala Gly Phe Thr Thr Arg Pro Pro Pro Pro Leu Glu Ile
420 425 430
ttg ttg caa cca tct tct cca tac ggt aga cca tac ttc aaa gct aag 1344
Leu Leu Gln Pro Ser Ser Pro Tyr Gly Arg Pro Tyr Phe Lys Ala Lys
435 440 445
tct gat ttc gtc aaa gaa ccc att cca gtt ttg ggt tta aag ggt atg 1392
Ser Asp Phe Val Lys Glu Pro Ile Pro Val Leu Gly Leu Lys Gly Met
450 455 460
ttc aag aga atg atc gaa gag gat gcc tct ttc ttg att tgg aaa cca 1440
Phe Lys Arg Met Ile Glu Glu Asp Ala Ser Phe Leu Ile Trp Lys Pro
465 470 475 480
tac ggt ggt atg atg gct aga att cca gaa tct gaa ttg cca ttt cca 1488
Tyr Gly Gly Met Met Ala Arg Ile Pro Glu Ser Glu Leu Pro Phe Pro
485 490 495
cac aga aac ggt act att ttc aag atc cat tac ttc acc aag tgg tcc 1536
His Arg Asn Gly Thr Ile Phe Lys Ile His Tyr Phe Thr Lys Trp Ser
500 505 510
gaa aat ggt tct tct tct gat gac aaa cac atg aag tgg atc agg gaa 1584
Glu Asn Gly Ser Ser Ser Asp Asp Lys His Met Lys Trp Ile Arg Glu
515 520 525
ctg tat tct tat atg acc cca tac gtt tct tct aac cca aga caa gct 1632
Leu Tyr Ser Tyr Met Thr Pro Tyr Val Ser Ser Asn Pro Arg Gln Ala
530 535 540
tac gtt aac tac aga gat ttg gac atc ggt cag aac aag aag aac gat 1680
Tyr Val Asn Tyr Arg Asp Leu Asp Ile Gly Gln Asn Lys Lys Asn Asp
545 550 555 560
tct gaa aac aat ttc aga agg gct caa att tgg ggt gct aag tac ttt 1728
Ser Glu Asn Asn Phe Arg Arg Ala Gln Ile Trp Gly Ala Lys Tyr Phe
565 570 575
aag aac aac ttc aac agg ctg gtc aag atc aag aca aaa gtt gat cca 1776
Lys Asn Asn Phe Asn Arg Leu Val Lys Ile Lys Thr Lys Val Asp Pro
580 585 590
gaa aac ttg ttc agg cac gaa caa tct att cca cca ttg cca att aga 1824
Glu Asn Leu Phe Arg His Glu Gln Ser Ile Pro Pro Leu Pro Ile Arg
595 600 605
agg caa acc tct tgg tca cat cca caa ttt gaa aag 1860
Arg Gln Thr Ser Trp Ser His Pro Gln Phe Glu Lys
610 615 620
<210> 125
<211> 620
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 125
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser
1 5 10 15
Ala Leu Ala Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln
20 25 30
Ile Pro Ala Glu Ala Val Ile Gly Tyr Ser Asp Leu Glu Gly Asp Phe
35 40 45
Asp Val Ala Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu
50 55 60
Phe Ile Asn Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val
65 70 75 80
Ser Leu Glu Lys Arg Glu Ala Glu Ala Met Lys Glu Pro Thr Ser Ser
85 90 95
Thr Ser Leu Gln Asp Asp Phe Val Lys Cys Leu His Arg Asn Thr Asn
100 105 110
Val Asp Phe Pro Leu Asp Lys Thr Phe Phe Thr Pro Gln Arg Asn Ser
115 120 125
Ser Asn Phe Lys Glu Val Leu Asn Ser Thr Val Trp Asn Gln Arg Tyr
130 135 140
Met Thr Lys Ser Met Pro Lys Pro Glu Phe Ile Phe Lys Pro Val His
145 150 155 160
Glu Ser His Val Gln Ala Ser Ile Ile Cys Ser Lys Lys Leu Arg Ile
165 170 175
His Phe Arg Val Arg Ser Gly Gly His Asp Tyr Glu Gly Leu Ser Tyr
180 185 190
Val Ser Gln Met Glu Thr Pro Phe Val Leu Ile Asp Leu Trp Lys Leu
195 200 205
Lys Gln Ile Asn Val Asp Ile Glu Asp Asn Ser Ala Trp Val Gln Ala
210 215 220
Gly Ala Thr Thr Gly Glu Leu Tyr Tyr Arg Ile Ala Glu Lys Ser Lys
225 230 235 240
Val His Gly Phe Pro Ala Gly Leu Cys Ser Thr Val Gly Ile Gly Gly
245 250 255
His Ile Thr Gly Gly Gly Tyr Gly Thr Leu Met Arg Lys Tyr Gly Leu
260 265 270
Ser Thr Asp Asn Val Leu Asp Ala Arg Ile Val Asp Ala Asn Gly Gln
275 280 285
Leu Leu Asn Arg Thr Ala Met Gly Glu Asp Leu Phe Trp Ala Ile Ser
290 295 300
Gly Gly Gly Gly Gly Ser Phe Gly Val Ile Leu Ala Trp Lys Ile Lys
305 310 315 320
Leu Val Pro Val Pro Glu Thr Leu Thr Ile Phe Thr Ile Thr Lys Thr
325 330 335
Leu Glu Gln Asp Ala Gly Phe Lys Ile Leu Ser Lys Trp Gln Arg Ile
340 345 350
Val Asp Lys Leu Val Glu Glu Leu Ser Ile Arg Val Leu Phe Arg Asn
355 360 365
Thr Gly Asn Lys Thr Val Thr Thr Thr Tyr Arg Gly Glu Phe Leu Gly
370 375 380
Glu Lys Gly Thr Leu Met Glu Val Met Asn Lys Cys Phe Pro Glu Leu
385 390 395 400
Gly Leu Thr Gln Glu Asp Cys Ile Glu Met Ser Trp Ile Glu Thr Val
405 410 415
Val Asn Asn Ala Gly Phe Thr Thr Arg Pro Pro Pro Pro Leu Glu Ile
420 425 430
Leu Leu Gln Pro Ser Ser Pro Tyr Gly Arg Pro Tyr Phe Lys Ala Lys
435 440 445
Ser Asp Phe Val Lys Glu Pro Ile Pro Val Leu Gly Leu Lys Gly Met
450 455 460
Phe Lys Arg Met Ile Glu Glu Asp Ala Ser Phe Leu Ile Trp Lys Pro
465 470 475 480
Tyr Gly Gly Met Met Ala Arg Ile Pro Glu Ser Glu Leu Pro Phe Pro
485 490 495
His Arg Asn Gly Thr Ile Phe Lys Ile His Tyr Phe Thr Lys Trp Ser
500 505 510
Glu Asn Gly Ser Ser Ser Asp Asp Lys His Met Lys Trp Ile Arg Glu
515 520 525
Leu Tyr Ser Tyr Met Thr Pro Tyr Val Ser Ser Asn Pro Arg Gln Ala
530 535 540
Tyr Val Asn Tyr Arg Asp Leu Asp Ile Gly Gln Asn Lys Lys Asn Asp
545 550 555 560
Ser Glu Asn Asn Phe Arg Arg Ala Gln Ile Trp Gly Ala Lys Tyr Phe
565 570 575
Lys Asn Asn Phe Asn Arg Leu Val Lys Ile Lys Thr Lys Val Asp Pro
580 585 590
Glu Asn Leu Phe Arg His Glu Gln Ser Ile Pro Pro Leu Pro Ile Arg
595 600 605
Arg Gln Thr Ser Trp Ser His Pro Gln Phe Glu Lys
610 615 620
<210> 126
<211> 1629
<212> DNA
<213> 人工序列
<220>
<223> CBS152-A0A1S3BE31
<220>
<221> CDS
<222> (1)..(1629)
<400> 126
atg aag tcc ttg atc acc tct ttc ttg cac atc ttc atc gtt ttg tct 48
Met Lys Ser Leu Ile Thr Ser Phe Leu His Ile Phe Ile Val Leu Ser
1 5 10 15
ttt gct tgg gct gct tct gct cat aat cat gaa tct ttc tta cac tgc 96
Phe Ala Trp Ala Ala Ser Ala His Asn His Glu Ser Phe Leu His Cys
20 25 30
ttg tcc cat cat tct cca aac acc tct tct atc tcc aag att atc tac 144
Leu Ser His His Ser Pro Asn Thr Ser Ser Ile Ser Lys Ile Ile Tyr
35 40 45
act cca act aac ccc tct tac tcc tct gtt ttg aac ttc tcc att cac 192
Thr Pro Thr Asn Pro Ser Tyr Ser Ser Val Leu Asn Phe Ser Ile His
50 55 60
aac ttg aga ttc acc tct cca aaa act cca aag cca cat att atc gtt 240
Asn Leu Arg Phe Thr Ser Pro Lys Thr Pro Lys Pro His Ile Ile Val
65 70 75 80
acc cca ttc ttg gtg tct caa att caa gcc tct att atc tgc gct aaa 288
Thr Pro Phe Leu Val Ser Gln Ile Gln Ala Ser Ile Ile Cys Ala Lys
85 90 95
aag acc ggt ttc caa atc aga act aga tct ggt ggt cat gat tac gaa 336
Lys Thr Gly Phe Gln Ile Arg Thr Arg Ser Gly Gly His Asp Tyr Glu
100 105 110
ggt ttg tct tac gtt tcc gat gtt tcc ttc gtt gtt gtt gac ttg att 384
Gly Leu Ser Tyr Val Ser Asp Val Ser Phe Val Val Val Asp Leu Ile
115 120 125
aac ctg aga tcc att tct gtt gat gcc gaa aac aat gtt gct tgg gtt 432
Asn Leu Arg Ser Ile Ser Val Asp Ala Glu Asn Asn Val Ala Trp Val
130 135 140
caa tct ggt gct act att ggt gag ttg tac tac aga att gcc gaa aag 480
Gln Ser Gly Ala Thr Ile Gly Glu Leu Tyr Tyr Arg Ile Ala Glu Lys
145 150 155 160
tct aag acc ttg ggt ttt cca gct ggt gtt tgt cca act gtt ggt gtt 528
Ser Lys Thr Leu Gly Phe Pro Ala Gly Val Cys Pro Thr Val Gly Val
165 170 175
ggt ggt cac ttt tct ggt ggt ggt tat ggt ttg atg ttg aga aaa ttt 576
Gly Gly His Phe Ser Gly Gly Gly Tyr Gly Leu Met Leu Arg Lys Phe
180 185 190
ggt ttg gcc gct gat aac gtc att gat gct tac ttc att gat gtc aac 624
Gly Leu Ala Ala Asp Asn Val Ile Asp Ala Tyr Phe Ile Asp Val Asn
195 200 205
ggt aag ctg cat gac aga aaa tct atg ggt gaa gat gtt ttc tgg gct 672
Gly Lys Leu His Asp Arg Lys Ser Met Gly Glu Asp Val Phe Trp Ala
210 215 220
att aga ggt ggt ggt ggc gct tct ttt ggt ata gtt ttg gct tgg aag 720
Ile Arg Gly Gly Gly Gly Ala Ser Phe Gly Ile Val Leu Ala Trp Lys
225 230 235 240
atc aag ttg ttg cca gtt cca tct atc gtt act gtt ttc act att acc 768
Ile Lys Leu Leu Pro Val Pro Ser Ile Val Thr Val Phe Thr Ile Thr
245 250 255
aga acc ttg gaa caa aac gcc acc gat ttg att cat aga tgg caa tac 816
Arg Thr Leu Glu Gln Asn Ala Thr Asp Leu Ile His Arg Trp Gln Tyr
260 265 270
gtt tct tct aag cag gat gac aag ttg ttc atc aga att atc ttg acc 864
Val Ser Ser Lys Gln Asp Asp Lys Leu Phe Ile Arg Ile Ile Leu Thr
275 280 285
ggt atc aac acc acc aat tca caa cat ggt aac aag aga act att gaa 912
Gly Ile Asn Thr Thr Asn Ser Gln His Gly Asn Lys Arg Thr Ile Glu
290 295 300
gct gcc ttc aac tct ttg ttc ttg ggt aag att gaa gag ttg gtc cca 960
Ala Ala Phe Asn Ser Leu Phe Leu Gly Lys Ile Glu Glu Leu Val Pro
305 310 315 320
att atg gaa aag act ttc cca gaa ttg ggt ttg acc aaa gaa gat tgc 1008
Ile Met Glu Lys Thr Phe Pro Glu Leu Gly Leu Thr Lys Glu Asp Cys
325 330 335
att gag atg tcc tgg att gag tct gtc ttg tac ttt gct ggt ttt tct 1056
Ile Glu Met Ser Trp Ile Glu Ser Val Leu Tyr Phe Ala Gly Phe Ser
340 345 350
aga ggt caa cca ttg aac gtt ttg ttg gat aga agg cca ttg act cca 1104
Arg Gly Gln Pro Leu Asn Val Leu Leu Asp Arg Arg Pro Leu Thr Pro
355 360 365
aag aga ttt ttc aag gct aag tcc gat tac gtc aac gaa cct att cca 1152
Lys Arg Phe Phe Lys Ala Lys Ser Asp Tyr Val Asn Glu Pro Ile Pro
370 375 380
aaa gct ggt ttg gaa ggt att tgg gag ttg ttc gat gaa gaa gaa gct 1200
Lys Ala Gly Leu Glu Gly Ile Trp Glu Leu Phe Asp Glu Glu Glu Ala
385 390 395 400
gaa gct gct gtt ttg att ttg tct cca tac ggt ggt atc atg gac aag 1248
Glu Ala Ala Val Leu Ile Leu Ser Pro Tyr Gly Gly Ile Met Asp Lys
405 410 415
att tcc gaa tcc gaa att cca ttt cca cat aga gct ggc aac ctg tac 1296
Ile Ser Glu Ser Glu Ile Pro Phe Pro His Arg Ala Gly Asn Leu Tyr
420 425 430
aaa atc caa cat ttg gtt tac tgg gac gaa gaa ggt gaa gat att gct 1344
Lys Ile Gln His Leu Val Tyr Trp Asp Glu Glu Gly Glu Asp Ile Ala
435 440 445
aag aga cat atc aac tgg atc aga aag ctg tac tct tac atg gct cca 1392
Lys Arg His Ile Asn Trp Ile Arg Lys Leu Tyr Ser Tyr Met Ala Pro
450 455 460
ttc gtt tct aaa aat cca aga gct gcc tac atc aac tac aga gat ttg 1440
Phe Val Ser Lys Asn Pro Arg Ala Ala Tyr Ile Asn Tyr Arg Asp Leu
465 470 475 480
gat att ggc acc aac gaa aag aac ggt aga aac act tct tac aac gaa 1488
Asp Ile Gly Thr Asn Glu Lys Asn Gly Arg Asn Thr Ser Tyr Asn Glu
485 490 495
gct tct gtt tgg ggt gtt aag tac ttt aag ggt aac ttc aag agg ctg 1536
Ala Ser Val Trp Gly Val Lys Tyr Phe Lys Gly Asn Phe Lys Arg Leu
500 505 510
gtt tct gtt aag aca aag gtt gac cca tcc aat ttc ttc aag aac gaa 1584
Val Ser Val Lys Thr Lys Val Asp Pro Ser Asn Phe Phe Lys Asn Glu
515 520 525
caa tcc att cca tcc ttg aaa cca tgg tgg aac aag aga ggt tat 1629
Gln Ser Ile Pro Ser Leu Lys Pro Trp Trp Asn Lys Arg Gly Tyr
530 535 540
<210> 127
<211> 543
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 127
Met Lys Ser Leu Ile Thr Ser Phe Leu His Ile Phe Ile Val Leu Ser
1 5 10 15
Phe Ala Trp Ala Ala Ser Ala His Asn His Glu Ser Phe Leu His Cys
20 25 30
Leu Ser His His Ser Pro Asn Thr Ser Ser Ile Ser Lys Ile Ile Tyr
35 40 45
Thr Pro Thr Asn Pro Ser Tyr Ser Ser Val Leu Asn Phe Ser Ile His
50 55 60
Asn Leu Arg Phe Thr Ser Pro Lys Thr Pro Lys Pro His Ile Ile Val
65 70 75 80
Thr Pro Phe Leu Val Ser Gln Ile Gln Ala Ser Ile Ile Cys Ala Lys
85 90 95
Lys Thr Gly Phe Gln Ile Arg Thr Arg Ser Gly Gly His Asp Tyr Glu
100 105 110
Gly Leu Ser Tyr Val Ser Asp Val Ser Phe Val Val Val Asp Leu Ile
115 120 125
Asn Leu Arg Ser Ile Ser Val Asp Ala Glu Asn Asn Val Ala Trp Val
130 135 140
Gln Ser Gly Ala Thr Ile Gly Glu Leu Tyr Tyr Arg Ile Ala Glu Lys
145 150 155 160
Ser Lys Thr Leu Gly Phe Pro Ala Gly Val Cys Pro Thr Val Gly Val
165 170 175
Gly Gly His Phe Ser Gly Gly Gly Tyr Gly Leu Met Leu Arg Lys Phe
180 185 190
Gly Leu Ala Ala Asp Asn Val Ile Asp Ala Tyr Phe Ile Asp Val Asn
195 200 205
Gly Lys Leu His Asp Arg Lys Ser Met Gly Glu Asp Val Phe Trp Ala
210 215 220
Ile Arg Gly Gly Gly Gly Ala Ser Phe Gly Ile Val Leu Ala Trp Lys
225 230 235 240
Ile Lys Leu Leu Pro Val Pro Ser Ile Val Thr Val Phe Thr Ile Thr
245 250 255
Arg Thr Leu Glu Gln Asn Ala Thr Asp Leu Ile His Arg Trp Gln Tyr
260 265 270
Val Ser Ser Lys Gln Asp Asp Lys Leu Phe Ile Arg Ile Ile Leu Thr
275 280 285
Gly Ile Asn Thr Thr Asn Ser Gln His Gly Asn Lys Arg Thr Ile Glu
290 295 300
Ala Ala Phe Asn Ser Leu Phe Leu Gly Lys Ile Glu Glu Leu Val Pro
305 310 315 320
Ile Met Glu Lys Thr Phe Pro Glu Leu Gly Leu Thr Lys Glu Asp Cys
325 330 335
Ile Glu Met Ser Trp Ile Glu Ser Val Leu Tyr Phe Ala Gly Phe Ser
340 345 350
Arg Gly Gln Pro Leu Asn Val Leu Leu Asp Arg Arg Pro Leu Thr Pro
355 360 365
Lys Arg Phe Phe Lys Ala Lys Ser Asp Tyr Val Asn Glu Pro Ile Pro
370 375 380
Lys Ala Gly Leu Glu Gly Ile Trp Glu Leu Phe Asp Glu Glu Glu Ala
385 390 395 400
Glu Ala Ala Val Leu Ile Leu Ser Pro Tyr Gly Gly Ile Met Asp Lys
405 410 415
Ile Ser Glu Ser Glu Ile Pro Phe Pro His Arg Ala Gly Asn Leu Tyr
420 425 430
Lys Ile Gln His Leu Val Tyr Trp Asp Glu Glu Gly Glu Asp Ile Ala
435 440 445
Lys Arg His Ile Asn Trp Ile Arg Lys Leu Tyr Ser Tyr Met Ala Pro
450 455 460
Phe Val Ser Lys Asn Pro Arg Ala Ala Tyr Ile Asn Tyr Arg Asp Leu
465 470 475 480
Asp Ile Gly Thr Asn Glu Lys Asn Gly Arg Asn Thr Ser Tyr Asn Glu
485 490 495
Ala Ser Val Trp Gly Val Lys Tyr Phe Lys Gly Asn Phe Lys Arg Leu
500 505 510
Val Ser Val Lys Thr Lys Val Asp Pro Ser Asn Phe Phe Lys Asn Glu
515 520 525
Gln Ser Ile Pro Ser Leu Lys Pro Trp Trp Asn Lys Arg Gly Tyr
530 535 540
<210> 128
<211> 1566
<212> DNA
<213> 人工序列
<220>
<223> CBS152-A0A1S3BE31-无信号肽
<220>
<221> CDS
<222> (1)..(1566)
<400> 128
atg gct cat aat cat gaa tct ttc tta cac tgc ttg tcc cat cat tct 48
Met Ala His Asn His Glu Ser Phe Leu His Cys Leu Ser His His Ser
1 5 10 15
cca aac acc tct tct atc tcc aag att atc tac act cca act aac ccc 96
Pro Asn Thr Ser Ser Ile Ser Lys Ile Ile Tyr Thr Pro Thr Asn Pro
20 25 30
tct tac tcc tct gtt ttg aac ttc tcc att cac aac ttg aga ttc acc 144
Ser Tyr Ser Ser Val Leu Asn Phe Ser Ile His Asn Leu Arg Phe Thr
35 40 45
tct cca aaa act cca aag cca cat att atc gtt acc cca ttc ttg gtg 192
Ser Pro Lys Thr Pro Lys Pro His Ile Ile Val Thr Pro Phe Leu Val
50 55 60
tct caa att caa gcc tct att atc tgc gct aaa aag acc ggt ttc caa 240
Ser Gln Ile Gln Ala Ser Ile Ile Cys Ala Lys Lys Thr Gly Phe Gln
65 70 75 80
atc aga act aga tct ggt ggt cat gat tac gaa ggt ttg tct tac gtt 288
Ile Arg Thr Arg Ser Gly Gly His Asp Tyr Glu Gly Leu Ser Tyr Val
85 90 95
tcc gat gtt tcc ttc gtt gtt gtt gac ttg att aac ctg aga tcc att 336
Ser Asp Val Ser Phe Val Val Val Asp Leu Ile Asn Leu Arg Ser Ile
100 105 110
tct gtt gat gcc gaa aac aat gtt gct tgg gtt caa tct ggt gct act 384
Ser Val Asp Ala Glu Asn Asn Val Ala Trp Val Gln Ser Gly Ala Thr
115 120 125
att ggt gag ttg tac tac aga att gcc gaa aag tct aag acc ttg ggt 432
Ile Gly Glu Leu Tyr Tyr Arg Ile Ala Glu Lys Ser Lys Thr Leu Gly
130 135 140
ttt cca gct ggt gtt tgt cca act gtt ggt gtt ggt ggt cac ttt tct 480
Phe Pro Ala Gly Val Cys Pro Thr Val Gly Val Gly Gly His Phe Ser
145 150 155 160
ggt ggt ggt tat ggt ttg atg ttg aga aaa ttt ggt ttg gcc gct gat 528
Gly Gly Gly Tyr Gly Leu Met Leu Arg Lys Phe Gly Leu Ala Ala Asp
165 170 175
aac gtc att gat gct tac ttc att gat gtc aac ggt aag ctg cat gac 576
Asn Val Ile Asp Ala Tyr Phe Ile Asp Val Asn Gly Lys Leu His Asp
180 185 190
aga aaa tct atg ggt gaa gat gtt ttc tgg gct att aga ggt ggt ggt 624
Arg Lys Ser Met Gly Glu Asp Val Phe Trp Ala Ile Arg Gly Gly Gly
195 200 205
ggc gct tct ttt ggt ata gtt ttg gct tgg aag atc aag ttg ttg cca 672
Gly Ala Ser Phe Gly Ile Val Leu Ala Trp Lys Ile Lys Leu Leu Pro
210 215 220
gtt cca tct atc gtt act gtt ttc act att acc aga acc ttg gaa caa 720
Val Pro Ser Ile Val Thr Val Phe Thr Ile Thr Arg Thr Leu Glu Gln
225 230 235 240
aac gcc acc gat ttg att cat aga tgg caa tac gtt tct tct aag cag 768
Asn Ala Thr Asp Leu Ile His Arg Trp Gln Tyr Val Ser Ser Lys Gln
245 250 255
gat gac aag ttg ttc atc aga att atc ttg acc ggt atc aac acc acc 816
Asp Asp Lys Leu Phe Ile Arg Ile Ile Leu Thr Gly Ile Asn Thr Thr
260 265 270
aat tca caa cat ggt aac aag aga act att gaa gct gcc ttc aac tct 864
Asn Ser Gln His Gly Asn Lys Arg Thr Ile Glu Ala Ala Phe Asn Ser
275 280 285
ttg ttc ttg ggt aag att gaa gag ttg gtc cca att atg gaa aag act 912
Leu Phe Leu Gly Lys Ile Glu Glu Leu Val Pro Ile Met Glu Lys Thr
290 295 300
ttc cca gaa ttg ggt ttg acc aaa gaa gat tgc att gag atg tcc tgg 960
Phe Pro Glu Leu Gly Leu Thr Lys Glu Asp Cys Ile Glu Met Ser Trp
305 310 315 320
att gag tct gtc ttg tac ttt gct ggt ttt tct aga ggt caa cca ttg 1008
Ile Glu Ser Val Leu Tyr Phe Ala Gly Phe Ser Arg Gly Gln Pro Leu
325 330 335
aac gtt ttg ttg gat aga agg cca ttg act cca aag aga ttt ttc aag 1056
Asn Val Leu Leu Asp Arg Arg Pro Leu Thr Pro Lys Arg Phe Phe Lys
340 345 350
gct aag tcc gat tac gtc aac gaa cct att cca aaa gct ggt ttg gaa 1104
Ala Lys Ser Asp Tyr Val Asn Glu Pro Ile Pro Lys Ala Gly Leu Glu
355 360 365
ggt att tgg gag ttg ttc gat gaa gaa gaa gct gaa gct gct gtt ttg 1152
Gly Ile Trp Glu Leu Phe Asp Glu Glu Glu Ala Glu Ala Ala Val Leu
370 375 380
att ttg tct cca tac ggt ggt atc atg gac aag att tcc gaa tcc gaa 1200
Ile Leu Ser Pro Tyr Gly Gly Ile Met Asp Lys Ile Ser Glu Ser Glu
385 390 395 400
att cca ttt cca cat aga gct ggc aac ctg tac aaa atc caa cat ttg 1248
Ile Pro Phe Pro His Arg Ala Gly Asn Leu Tyr Lys Ile Gln His Leu
405 410 415
gtt tac tgg gac gaa gaa ggt gaa gat att gct aag aga cat atc aac 1296
Val Tyr Trp Asp Glu Glu Gly Glu Asp Ile Ala Lys Arg His Ile Asn
420 425 430
tgg atc aga aag ctg tac tct tac atg gct cca ttc gtt tct aaa aat 1344
Trp Ile Arg Lys Leu Tyr Ser Tyr Met Ala Pro Phe Val Ser Lys Asn
435 440 445
cca aga gct gcc tac atc aac tac aga gat ttg gat att ggc acc aac 1392
Pro Arg Ala Ala Tyr Ile Asn Tyr Arg Asp Leu Asp Ile Gly Thr Asn
450 455 460
gaa aag aac ggt aga aac act tct tac aac gaa gct tct gtt tgg ggt 1440
Glu Lys Asn Gly Arg Asn Thr Ser Tyr Asn Glu Ala Ser Val Trp Gly
465 470 475 480
gtt aag tac ttt aag ggt aac ttc aag agg ctg gtt tct gtt aag aca 1488
Val Lys Tyr Phe Lys Gly Asn Phe Lys Arg Leu Val Ser Val Lys Thr
485 490 495
aag gtt gac cca tcc aat ttc ttc aag aac gaa caa tcc att cca tcc 1536
Lys Val Asp Pro Ser Asn Phe Phe Lys Asn Glu Gln Ser Ile Pro Ser
500 505 510
ttg aaa cca tgg tgg aac aag aga ggt tat 1566
Leu Lys Pro Trp Trp Asn Lys Arg Gly Tyr
515 520
<210> 129
<211> 522
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 129
Met Ala His Asn His Glu Ser Phe Leu His Cys Leu Ser His His Ser
1 5 10 15
Pro Asn Thr Ser Ser Ile Ser Lys Ile Ile Tyr Thr Pro Thr Asn Pro
20 25 30
Ser Tyr Ser Ser Val Leu Asn Phe Ser Ile His Asn Leu Arg Phe Thr
35 40 45
Ser Pro Lys Thr Pro Lys Pro His Ile Ile Val Thr Pro Phe Leu Val
50 55 60
Ser Gln Ile Gln Ala Ser Ile Ile Cys Ala Lys Lys Thr Gly Phe Gln
65 70 75 80
Ile Arg Thr Arg Ser Gly Gly His Asp Tyr Glu Gly Leu Ser Tyr Val
85 90 95
Ser Asp Val Ser Phe Val Val Val Asp Leu Ile Asn Leu Arg Ser Ile
100 105 110
Ser Val Asp Ala Glu Asn Asn Val Ala Trp Val Gln Ser Gly Ala Thr
115 120 125
Ile Gly Glu Leu Tyr Tyr Arg Ile Ala Glu Lys Ser Lys Thr Leu Gly
130 135 140
Phe Pro Ala Gly Val Cys Pro Thr Val Gly Val Gly Gly His Phe Ser
145 150 155 160
Gly Gly Gly Tyr Gly Leu Met Leu Arg Lys Phe Gly Leu Ala Ala Asp
165 170 175
Asn Val Ile Asp Ala Tyr Phe Ile Asp Val Asn Gly Lys Leu His Asp
180 185 190
Arg Lys Ser Met Gly Glu Asp Val Phe Trp Ala Ile Arg Gly Gly Gly
195 200 205
Gly Ala Ser Phe Gly Ile Val Leu Ala Trp Lys Ile Lys Leu Leu Pro
210 215 220
Val Pro Ser Ile Val Thr Val Phe Thr Ile Thr Arg Thr Leu Glu Gln
225 230 235 240
Asn Ala Thr Asp Leu Ile His Arg Trp Gln Tyr Val Ser Ser Lys Gln
245 250 255
Asp Asp Lys Leu Phe Ile Arg Ile Ile Leu Thr Gly Ile Asn Thr Thr
260 265 270
Asn Ser Gln His Gly Asn Lys Arg Thr Ile Glu Ala Ala Phe Asn Ser
275 280 285
Leu Phe Leu Gly Lys Ile Glu Glu Leu Val Pro Ile Met Glu Lys Thr
290 295 300
Phe Pro Glu Leu Gly Leu Thr Lys Glu Asp Cys Ile Glu Met Ser Trp
305 310 315 320
Ile Glu Ser Val Leu Tyr Phe Ala Gly Phe Ser Arg Gly Gln Pro Leu
325 330 335
Asn Val Leu Leu Asp Arg Arg Pro Leu Thr Pro Lys Arg Phe Phe Lys
340 345 350
Ala Lys Ser Asp Tyr Val Asn Glu Pro Ile Pro Lys Ala Gly Leu Glu
355 360 365
Gly Ile Trp Glu Leu Phe Asp Glu Glu Glu Ala Glu Ala Ala Val Leu
370 375 380
Ile Leu Ser Pro Tyr Gly Gly Ile Met Asp Lys Ile Ser Glu Ser Glu
385 390 395 400
Ile Pro Phe Pro His Arg Ala Gly Asn Leu Tyr Lys Ile Gln His Leu
405 410 415
Val Tyr Trp Asp Glu Glu Gly Glu Asp Ile Ala Lys Arg His Ile Asn
420 425 430
Trp Ile Arg Lys Leu Tyr Ser Tyr Met Ala Pro Phe Val Ser Lys Asn
435 440 445
Pro Arg Ala Ala Tyr Ile Asn Tyr Arg Asp Leu Asp Ile Gly Thr Asn
450 455 460
Glu Lys Asn Gly Arg Asn Thr Ser Tyr Asn Glu Ala Ser Val Trp Gly
465 470 475 480
Val Lys Tyr Phe Lys Gly Asn Phe Lys Arg Leu Val Ser Val Lys Thr
485 490 495
Lys Val Asp Pro Ser Asn Phe Phe Lys Asn Glu Gln Ser Ile Pro Ser
500 505 510
Leu Lys Pro Trp Trp Asn Lys Arg Gly Tyr
515 520
<210> 130
<211> 1590
<212> DNA
<213> 人工序列
<220>
<223> CBS152-A0A1S3BE31-无信号肽-strep标签
<220>
<221> CDS
<222> (1)..(1590)
<400> 130
atg gct cat aat cat gaa tct ttc tta cac tgc ttg tcc cat cat tct 48
Met Ala His Asn His Glu Ser Phe Leu His Cys Leu Ser His His Ser
1 5 10 15
cca aac acc tct tct atc tcc aag att atc tac act cca act aac ccc 96
Pro Asn Thr Ser Ser Ile Ser Lys Ile Ile Tyr Thr Pro Thr Asn Pro
20 25 30
tct tac tcc tct gtt ttg aac ttc tcc att cac aac ttg aga ttc acc 144
Ser Tyr Ser Ser Val Leu Asn Phe Ser Ile His Asn Leu Arg Phe Thr
35 40 45
tct cca aaa act cca aag cca cat att atc gtt acc cca ttc ttg gtg 192
Ser Pro Lys Thr Pro Lys Pro His Ile Ile Val Thr Pro Phe Leu Val
50 55 60
tct caa att caa gcc tct att atc tgc gct aaa aag acc ggt ttc caa 240
Ser Gln Ile Gln Ala Ser Ile Ile Cys Ala Lys Lys Thr Gly Phe Gln
65 70 75 80
atc aga act aga tct ggt ggt cat gat tac gaa ggt ttg tct tac gtt 288
Ile Arg Thr Arg Ser Gly Gly His Asp Tyr Glu Gly Leu Ser Tyr Val
85 90 95
tcc gat gtt tcc ttc gtt gtt gtt gac ttg att aac ctg aga tcc att 336
Ser Asp Val Ser Phe Val Val Val Asp Leu Ile Asn Leu Arg Ser Ile
100 105 110
tct gtt gat gcc gaa aac aat gtt gct tgg gtt caa tct ggt gct act 384
Ser Val Asp Ala Glu Asn Asn Val Ala Trp Val Gln Ser Gly Ala Thr
115 120 125
att ggt gag ttg tac tac aga att gcc gaa aag tct aag acc ttg ggt 432
Ile Gly Glu Leu Tyr Tyr Arg Ile Ala Glu Lys Ser Lys Thr Leu Gly
130 135 140
ttt cca gct ggt gtt tgt cca act gtt ggt gtt ggt ggt cac ttt tct 480
Phe Pro Ala Gly Val Cys Pro Thr Val Gly Val Gly Gly His Phe Ser
145 150 155 160
ggt ggt ggt tat ggt ttg atg ttg aga aaa ttt ggt ttg gcc gct gat 528
Gly Gly Gly Tyr Gly Leu Met Leu Arg Lys Phe Gly Leu Ala Ala Asp
165 170 175
aac gtc att gat gct tac ttc att gat gtc aac ggt aag ctg cat gac 576
Asn Val Ile Asp Ala Tyr Phe Ile Asp Val Asn Gly Lys Leu His Asp
180 185 190
aga aaa tct atg ggt gaa gat gtt ttc tgg gct att aga ggt ggt ggt 624
Arg Lys Ser Met Gly Glu Asp Val Phe Trp Ala Ile Arg Gly Gly Gly
195 200 205
ggc gct tct ttt ggt ata gtt ttg gct tgg aag atc aag ttg ttg cca 672
Gly Ala Ser Phe Gly Ile Val Leu Ala Trp Lys Ile Lys Leu Leu Pro
210 215 220
gtt cca tct atc gtt act gtt ttc act att acc aga acc ttg gaa caa 720
Val Pro Ser Ile Val Thr Val Phe Thr Ile Thr Arg Thr Leu Glu Gln
225 230 235 240
aac gcc acc gat ttg att cat aga tgg caa tac gtt tct tct aag cag 768
Asn Ala Thr Asp Leu Ile His Arg Trp Gln Tyr Val Ser Ser Lys Gln
245 250 255
gat gac aag ttg ttc atc aga att atc ttg acc ggt atc aac acc acc 816
Asp Asp Lys Leu Phe Ile Arg Ile Ile Leu Thr Gly Ile Asn Thr Thr
260 265 270
aat tca caa cat ggt aac aag aga act att gaa gct gcc ttc aac tct 864
Asn Ser Gln His Gly Asn Lys Arg Thr Ile Glu Ala Ala Phe Asn Ser
275 280 285
ttg ttc ttg ggt aag att gaa gag ttg gtc cca att atg gaa aag act 912
Leu Phe Leu Gly Lys Ile Glu Glu Leu Val Pro Ile Met Glu Lys Thr
290 295 300
ttc cca gaa ttg ggt ttg acc aaa gaa gat tgc att gag atg tcc tgg 960
Phe Pro Glu Leu Gly Leu Thr Lys Glu Asp Cys Ile Glu Met Ser Trp
305 310 315 320
att gag tct gtc ttg tac ttt gct ggt ttt tct aga ggt caa cca ttg 1008
Ile Glu Ser Val Leu Tyr Phe Ala Gly Phe Ser Arg Gly Gln Pro Leu
325 330 335
aac gtt ttg ttg gat aga agg cca ttg act cca aag aga ttt ttc aag 1056
Asn Val Leu Leu Asp Arg Arg Pro Leu Thr Pro Lys Arg Phe Phe Lys
340 345 350
gct aag tcc gat tac gtc aac gaa cct att cca aaa gct ggt ttg gaa 1104
Ala Lys Ser Asp Tyr Val Asn Glu Pro Ile Pro Lys Ala Gly Leu Glu
355 360 365
ggt att tgg gag ttg ttc gat gaa gaa gaa gct gaa gct gct gtt ttg 1152
Gly Ile Trp Glu Leu Phe Asp Glu Glu Glu Ala Glu Ala Ala Val Leu
370 375 380
att ttg tct cca tac ggt ggt atc atg gac aag att tcc gaa tcc gaa 1200
Ile Leu Ser Pro Tyr Gly Gly Ile Met Asp Lys Ile Ser Glu Ser Glu
385 390 395 400
att cca ttt cca cat aga gct ggc aac ctg tac aaa atc caa cat ttg 1248
Ile Pro Phe Pro His Arg Ala Gly Asn Leu Tyr Lys Ile Gln His Leu
405 410 415
gtt tac tgg gac gaa gaa ggt gaa gat att gct aag aga cat atc aac 1296
Val Tyr Trp Asp Glu Glu Gly Glu Asp Ile Ala Lys Arg His Ile Asn
420 425 430
tgg atc aga aag ctg tac tct tac atg gct cca ttc gtt tct aaa aat 1344
Trp Ile Arg Lys Leu Tyr Ser Tyr Met Ala Pro Phe Val Ser Lys Asn
435 440 445
cca aga gct gcc tac atc aac tac aga gat ttg gat att ggc acc aac 1392
Pro Arg Ala Ala Tyr Ile Asn Tyr Arg Asp Leu Asp Ile Gly Thr Asn
450 455 460
gaa aag aac ggt aga aac act tct tac aac gaa gct tct gtt tgg ggt 1440
Glu Lys Asn Gly Arg Asn Thr Ser Tyr Asn Glu Ala Ser Val Trp Gly
465 470 475 480
gtt aag tac ttt aag ggt aac ttc aag agg ctg gtt tct gtt aag aca 1488
Val Lys Tyr Phe Lys Gly Asn Phe Lys Arg Leu Val Ser Val Lys Thr
485 490 495
aag gtt gac cca tcc aat ttc ttc aag aac gaa caa tcc att cca tcc 1536
Lys Val Asp Pro Ser Asn Phe Phe Lys Asn Glu Gln Ser Ile Pro Ser
500 505 510
ttg aaa cca tgg tgg aac aag aga ggt tat tgg tca cat cca caa ttt 1584
Leu Lys Pro Trp Trp Asn Lys Arg Gly Tyr Trp Ser His Pro Gln Phe
515 520 525
gaa aag 1590
Glu Lys
530
<210> 131
<211> 530
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 131
Met Ala His Asn His Glu Ser Phe Leu His Cys Leu Ser His His Ser
1 5 10 15
Pro Asn Thr Ser Ser Ile Ser Lys Ile Ile Tyr Thr Pro Thr Asn Pro
20 25 30
Ser Tyr Ser Ser Val Leu Asn Phe Ser Ile His Asn Leu Arg Phe Thr
35 40 45
Ser Pro Lys Thr Pro Lys Pro His Ile Ile Val Thr Pro Phe Leu Val
50 55 60
Ser Gln Ile Gln Ala Ser Ile Ile Cys Ala Lys Lys Thr Gly Phe Gln
65 70 75 80
Ile Arg Thr Arg Ser Gly Gly His Asp Tyr Glu Gly Leu Ser Tyr Val
85 90 95
Ser Asp Val Ser Phe Val Val Val Asp Leu Ile Asn Leu Arg Ser Ile
100 105 110
Ser Val Asp Ala Glu Asn Asn Val Ala Trp Val Gln Ser Gly Ala Thr
115 120 125
Ile Gly Glu Leu Tyr Tyr Arg Ile Ala Glu Lys Ser Lys Thr Leu Gly
130 135 140
Phe Pro Ala Gly Val Cys Pro Thr Val Gly Val Gly Gly His Phe Ser
145 150 155 160
Gly Gly Gly Tyr Gly Leu Met Leu Arg Lys Phe Gly Leu Ala Ala Asp
165 170 175
Asn Val Ile Asp Ala Tyr Phe Ile Asp Val Asn Gly Lys Leu His Asp
180 185 190
Arg Lys Ser Met Gly Glu Asp Val Phe Trp Ala Ile Arg Gly Gly Gly
195 200 205
Gly Ala Ser Phe Gly Ile Val Leu Ala Trp Lys Ile Lys Leu Leu Pro
210 215 220
Val Pro Ser Ile Val Thr Val Phe Thr Ile Thr Arg Thr Leu Glu Gln
225 230 235 240
Asn Ala Thr Asp Leu Ile His Arg Trp Gln Tyr Val Ser Ser Lys Gln
245 250 255
Asp Asp Lys Leu Phe Ile Arg Ile Ile Leu Thr Gly Ile Asn Thr Thr
260 265 270
Asn Ser Gln His Gly Asn Lys Arg Thr Ile Glu Ala Ala Phe Asn Ser
275 280 285
Leu Phe Leu Gly Lys Ile Glu Glu Leu Val Pro Ile Met Glu Lys Thr
290 295 300
Phe Pro Glu Leu Gly Leu Thr Lys Glu Asp Cys Ile Glu Met Ser Trp
305 310 315 320
Ile Glu Ser Val Leu Tyr Phe Ala Gly Phe Ser Arg Gly Gln Pro Leu
325 330 335
Asn Val Leu Leu Asp Arg Arg Pro Leu Thr Pro Lys Arg Phe Phe Lys
340 345 350
Ala Lys Ser Asp Tyr Val Asn Glu Pro Ile Pro Lys Ala Gly Leu Glu
355 360 365
Gly Ile Trp Glu Leu Phe Asp Glu Glu Glu Ala Glu Ala Ala Val Leu
370 375 380
Ile Leu Ser Pro Tyr Gly Gly Ile Met Asp Lys Ile Ser Glu Ser Glu
385 390 395 400
Ile Pro Phe Pro His Arg Ala Gly Asn Leu Tyr Lys Ile Gln His Leu
405 410 415
Val Tyr Trp Asp Glu Glu Gly Glu Asp Ile Ala Lys Arg His Ile Asn
420 425 430
Trp Ile Arg Lys Leu Tyr Ser Tyr Met Ala Pro Phe Val Ser Lys Asn
435 440 445
Pro Arg Ala Ala Tyr Ile Asn Tyr Arg Asp Leu Asp Ile Gly Thr Asn
450 455 460
Glu Lys Asn Gly Arg Asn Thr Ser Tyr Asn Glu Ala Ser Val Trp Gly
465 470 475 480
Val Lys Tyr Phe Lys Gly Asn Phe Lys Arg Leu Val Ser Val Lys Thr
485 490 495
Lys Val Asp Pro Ser Asn Phe Phe Lys Asn Glu Gln Ser Ile Pro Ser
500 505 510
Leu Lys Pro Trp Trp Asn Lys Arg Gly Tyr Trp Ser His Pro Gln Phe
515 520 525
Glu Lys
530
<210> 132
<211> 1647
<212> DNA
<213> 人工序列
<220>
<223> CBS152-A0A1S3BE31-无信号肽-截断alpha因子-strep标签
<220>
<221> CDS
<222> (1)..(1647)
<400> 132
atg aga ttt cct tcc atc ttc acg gct gtg cta ttt gca gca tcc tcc 48
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser
1 5 10 15
gca ctt gca atg gct cat aat cat gaa tct ttc tta cac tgc ttg tcc 96
Ala Leu Ala Met Ala His Asn His Glu Ser Phe Leu His Cys Leu Ser
20 25 30
cat cat tct cca aac acc tct tct atc tcc aag att atc tac act cca 144
His His Ser Pro Asn Thr Ser Ser Ile Ser Lys Ile Ile Tyr Thr Pro
35 40 45
act aac ccc tct tac tcc tct gtt ttg aac ttc tcc att cac aac ttg 192
Thr Asn Pro Ser Tyr Ser Ser Val Leu Asn Phe Ser Ile His Asn Leu
50 55 60
aga ttc acc tct cca aaa act cca aag cca cat att atc gtt acc cca 240
Arg Phe Thr Ser Pro Lys Thr Pro Lys Pro His Ile Ile Val Thr Pro
65 70 75 80
ttc ttg gtg tct caa att caa gcc tct att atc tgc gct aaa aag acc 288
Phe Leu Val Ser Gln Ile Gln Ala Ser Ile Ile Cys Ala Lys Lys Thr
85 90 95
ggt ttc caa atc aga act aga tct ggt ggt cat gat tac gaa ggt ttg 336
Gly Phe Gln Ile Arg Thr Arg Ser Gly Gly His Asp Tyr Glu Gly Leu
100 105 110
tct tac gtt tcc gat gtt tcc ttc gtt gtt gtt gac ttg att aac ctg 384
Ser Tyr Val Ser Asp Val Ser Phe Val Val Val Asp Leu Ile Asn Leu
115 120 125
aga tcc att tct gtt gat gcc gaa aac aat gtt gct tgg gtt caa tct 432
Arg Ser Ile Ser Val Asp Ala Glu Asn Asn Val Ala Trp Val Gln Ser
130 135 140
ggt gct act att ggt gag ttg tac tac aga att gcc gaa aag tct aag 480
Gly Ala Thr Ile Gly Glu Leu Tyr Tyr Arg Ile Ala Glu Lys Ser Lys
145 150 155 160
acc ttg ggt ttt cca gct ggt gtt tgt cca act gtt ggt gtt ggt ggt 528
Thr Leu Gly Phe Pro Ala Gly Val Cys Pro Thr Val Gly Val Gly Gly
165 170 175
cac ttt tct ggt ggt ggt tat ggt ttg atg ttg aga aaa ttt ggt ttg 576
His Phe Ser Gly Gly Gly Tyr Gly Leu Met Leu Arg Lys Phe Gly Leu
180 185 190
gcc gct gat aac gtc att gat gct tac ttc att gat gtc aac ggt aag 624
Ala Ala Asp Asn Val Ile Asp Ala Tyr Phe Ile Asp Val Asn Gly Lys
195 200 205
ctg cat gac aga aaa tct atg ggt gaa gat gtt ttc tgg gct att aga 672
Leu His Asp Arg Lys Ser Met Gly Glu Asp Val Phe Trp Ala Ile Arg
210 215 220
ggt ggt ggt ggc gct tct ttt ggt ata gtt ttg gct tgg aag atc aag 720
Gly Gly Gly Gly Ala Ser Phe Gly Ile Val Leu Ala Trp Lys Ile Lys
225 230 235 240
ttg ttg cca gtt cca tct atc gtt act gtt ttc act att acc aga acc 768
Leu Leu Pro Val Pro Ser Ile Val Thr Val Phe Thr Ile Thr Arg Thr
245 250 255
ttg gaa caa aac gcc acc gat ttg att cat aga tgg caa tac gtt tct 816
Leu Glu Gln Asn Ala Thr Asp Leu Ile His Arg Trp Gln Tyr Val Ser
260 265 270
tct aag cag gat gac aag ttg ttc atc aga att atc ttg acc ggt atc 864
Ser Lys Gln Asp Asp Lys Leu Phe Ile Arg Ile Ile Leu Thr Gly Ile
275 280 285
aac acc acc aat tca caa cat ggt aac aag aga act att gaa gct gcc 912
Asn Thr Thr Asn Ser Gln His Gly Asn Lys Arg Thr Ile Glu Ala Ala
290 295 300
ttc aac tct ttg ttc ttg ggt aag att gaa gag ttg gtc cca att atg 960
Phe Asn Ser Leu Phe Leu Gly Lys Ile Glu Glu Leu Val Pro Ile Met
305 310 315 320
gaa aag act ttc cca gaa ttg ggt ttg acc aaa gaa gat tgc att gag 1008
Glu Lys Thr Phe Pro Glu Leu Gly Leu Thr Lys Glu Asp Cys Ile Glu
325 330 335
atg tcc tgg att gag tct gtc ttg tac ttt gct ggt ttt tct aga ggt 1056
Met Ser Trp Ile Glu Ser Val Leu Tyr Phe Ala Gly Phe Ser Arg Gly
340 345 350
caa cca ttg aac gtt ttg ttg gat aga agg cca ttg act cca aag aga 1104
Gln Pro Leu Asn Val Leu Leu Asp Arg Arg Pro Leu Thr Pro Lys Arg
355 360 365
ttt ttc aag gct aag tcc gat tac gtc aac gaa cct att cca aaa gct 1152
Phe Phe Lys Ala Lys Ser Asp Tyr Val Asn Glu Pro Ile Pro Lys Ala
370 375 380
ggt ttg gaa ggt att tgg gag ttg ttc gat gaa gaa gaa gct gaa gct 1200
Gly Leu Glu Gly Ile Trp Glu Leu Phe Asp Glu Glu Glu Ala Glu Ala
385 390 395 400
gct gtt ttg att ttg tct cca tac ggt ggt atc atg gac aag att tcc 1248
Ala Val Leu Ile Leu Ser Pro Tyr Gly Gly Ile Met Asp Lys Ile Ser
405 410 415
gaa tcc gaa att cca ttt cca cat aga gct ggc aac ctg tac aaa atc 1296
Glu Ser Glu Ile Pro Phe Pro His Arg Ala Gly Asn Leu Tyr Lys Ile
420 425 430
caa cat ttg gtt tac tgg gac gaa gaa ggt gaa gat att gct aag aga 1344
Gln His Leu Val Tyr Trp Asp Glu Glu Gly Glu Asp Ile Ala Lys Arg
435 440 445
cat atc aac tgg atc aga aag ctg tac tct tac atg gct cca ttc gtt 1392
His Ile Asn Trp Ile Arg Lys Leu Tyr Ser Tyr Met Ala Pro Phe Val
450 455 460
tct aaa aat cca aga gct gcc tac atc aac tac aga gat ttg gat att 1440
Ser Lys Asn Pro Arg Ala Ala Tyr Ile Asn Tyr Arg Asp Leu Asp Ile
465 470 475 480
ggc acc aac gaa aag aac ggt aga aac act tct tac aac gaa gct tct 1488
Gly Thr Asn Glu Lys Asn Gly Arg Asn Thr Ser Tyr Asn Glu Ala Ser
485 490 495
gtt tgg ggt gtt aag tac ttt aag ggt aac ttc aag agg ctg gtt tct 1536
Val Trp Gly Val Lys Tyr Phe Lys Gly Asn Phe Lys Arg Leu Val Ser
500 505 510
gtt aag aca aag gtt gac cca tcc aat ttc ttc aag aac gaa caa tcc 1584
Val Lys Thr Lys Val Asp Pro Ser Asn Phe Phe Lys Asn Glu Gln Ser
515 520 525
att cca tcc ttg aaa cca tgg tgg aac aag aga ggt tat tgg tca cat 1632
Ile Pro Ser Leu Lys Pro Trp Trp Asn Lys Arg Gly Tyr Trp Ser His
530 535 540
cca caa ttt gaa aag 1647
Pro Gln Phe Glu Lys
545
<210> 133
<211> 549
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 133
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser
1 5 10 15
Ala Leu Ala Met Ala His Asn His Glu Ser Phe Leu His Cys Leu Ser
20 25 30
His His Ser Pro Asn Thr Ser Ser Ile Ser Lys Ile Ile Tyr Thr Pro
35 40 45
Thr Asn Pro Ser Tyr Ser Ser Val Leu Asn Phe Ser Ile His Asn Leu
50 55 60
Arg Phe Thr Ser Pro Lys Thr Pro Lys Pro His Ile Ile Val Thr Pro
65 70 75 80
Phe Leu Val Ser Gln Ile Gln Ala Ser Ile Ile Cys Ala Lys Lys Thr
85 90 95
Gly Phe Gln Ile Arg Thr Arg Ser Gly Gly His Asp Tyr Glu Gly Leu
100 105 110
Ser Tyr Val Ser Asp Val Ser Phe Val Val Val Asp Leu Ile Asn Leu
115 120 125
Arg Ser Ile Ser Val Asp Ala Glu Asn Asn Val Ala Trp Val Gln Ser
130 135 140
Gly Ala Thr Ile Gly Glu Leu Tyr Tyr Arg Ile Ala Glu Lys Ser Lys
145 150 155 160
Thr Leu Gly Phe Pro Ala Gly Val Cys Pro Thr Val Gly Val Gly Gly
165 170 175
His Phe Ser Gly Gly Gly Tyr Gly Leu Met Leu Arg Lys Phe Gly Leu
180 185 190
Ala Ala Asp Asn Val Ile Asp Ala Tyr Phe Ile Asp Val Asn Gly Lys
195 200 205
Leu His Asp Arg Lys Ser Met Gly Glu Asp Val Phe Trp Ala Ile Arg
210 215 220
Gly Gly Gly Gly Ala Ser Phe Gly Ile Val Leu Ala Trp Lys Ile Lys
225 230 235 240
Leu Leu Pro Val Pro Ser Ile Val Thr Val Phe Thr Ile Thr Arg Thr
245 250 255
Leu Glu Gln Asn Ala Thr Asp Leu Ile His Arg Trp Gln Tyr Val Ser
260 265 270
Ser Lys Gln Asp Asp Lys Leu Phe Ile Arg Ile Ile Leu Thr Gly Ile
275 280 285
Asn Thr Thr Asn Ser Gln His Gly Asn Lys Arg Thr Ile Glu Ala Ala
290 295 300
Phe Asn Ser Leu Phe Leu Gly Lys Ile Glu Glu Leu Val Pro Ile Met
305 310 315 320
Glu Lys Thr Phe Pro Glu Leu Gly Leu Thr Lys Glu Asp Cys Ile Glu
325 330 335
Met Ser Trp Ile Glu Ser Val Leu Tyr Phe Ala Gly Phe Ser Arg Gly
340 345 350
Gln Pro Leu Asn Val Leu Leu Asp Arg Arg Pro Leu Thr Pro Lys Arg
355 360 365
Phe Phe Lys Ala Lys Ser Asp Tyr Val Asn Glu Pro Ile Pro Lys Ala
370 375 380
Gly Leu Glu Gly Ile Trp Glu Leu Phe Asp Glu Glu Glu Ala Glu Ala
385 390 395 400
Ala Val Leu Ile Leu Ser Pro Tyr Gly Gly Ile Met Asp Lys Ile Ser
405 410 415
Glu Ser Glu Ile Pro Phe Pro His Arg Ala Gly Asn Leu Tyr Lys Ile
420 425 430
Gln His Leu Val Tyr Trp Asp Glu Glu Gly Glu Asp Ile Ala Lys Arg
435 440 445
His Ile Asn Trp Ile Arg Lys Leu Tyr Ser Tyr Met Ala Pro Phe Val
450 455 460
Ser Lys Asn Pro Arg Ala Ala Tyr Ile Asn Tyr Arg Asp Leu Asp Ile
465 470 475 480
Gly Thr Asn Glu Lys Asn Gly Arg Asn Thr Ser Tyr Asn Glu Ala Ser
485 490 495
Val Trp Gly Val Lys Tyr Phe Lys Gly Asn Phe Lys Arg Leu Val Ser
500 505 510
Val Lys Thr Lys Val Asp Pro Ser Asn Phe Phe Lys Asn Glu Gln Ser
515 520 525
Ile Pro Ser Leu Lys Pro Trp Trp Asn Lys Arg Gly Tyr Trp Ser His
530 535 540
Pro Gln Phe Glu Lys
545
<210> 134
<211> 1857
<212> DNA
<213> 人工序列
<220>
<223> CBS152-A0A1S3BE31-无信号肽-全长alpha因子-strep标签
<220>
<221> CDS
<222> (1)..(1857)
<400> 134
atg aga ttt cct tca att ttt act gct gtt tta ttc gca gca tcc tcc 48
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser
1 5 10 15
gca tta gct gct cca gtc aac act aca aca gaa gat gaa acg gca caa 96
Ala Leu Ala Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln
20 25 30
att ccg gct gaa gct gtc atc ggt tac tca gat tta gaa ggg gat ttc 144
Ile Pro Ala Glu Ala Val Ile Gly Tyr Ser Asp Leu Glu Gly Asp Phe
35 40 45
gat gtt gct gtt ttg cca ttt tcc aac agc aca aat aac ggg tta ttg 192
Asp Val Ala Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu
50 55 60
ttt ata aat act act att gcc agc att gct gct aaa gaa gaa ggg gta 240
Phe Ile Asn Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val
65 70 75 80
tct ctc gag aaa aga gag gct gaa gct atg gct cat aat cat gaa tct 288
Ser Leu Glu Lys Arg Glu Ala Glu Ala Met Ala His Asn His Glu Ser
85 90 95
ttc tta cac tgc ttg tcc cat cat tct cca aac acc tct tct atc tcc 336
Phe Leu His Cys Leu Ser His His Ser Pro Asn Thr Ser Ser Ile Ser
100 105 110
aag att atc tac act cca act aac ccc tct tac tcc tct gtt ttg aac 384
Lys Ile Ile Tyr Thr Pro Thr Asn Pro Ser Tyr Ser Ser Val Leu Asn
115 120 125
ttc tcc att cac aac ttg aga ttc acc tct cca aaa act cca aag cca 432
Phe Ser Ile His Asn Leu Arg Phe Thr Ser Pro Lys Thr Pro Lys Pro
130 135 140
cat att atc gtt acc cca ttc ttg gtg tct caa att caa gcc tct att 480
His Ile Ile Val Thr Pro Phe Leu Val Ser Gln Ile Gln Ala Ser Ile
145 150 155 160
atc tgc gct aaa aag acc ggt ttc caa atc aga act aga tct ggt ggt 528
Ile Cys Ala Lys Lys Thr Gly Phe Gln Ile Arg Thr Arg Ser Gly Gly
165 170 175
cat gat tac gaa ggt ttg tct tac gtt tcc gat gtt tcc ttc gtt gtt 576
His Asp Tyr Glu Gly Leu Ser Tyr Val Ser Asp Val Ser Phe Val Val
180 185 190
gtt gac ttg att aac ctg aga tcc att tct gtt gat gcc gaa aac aat 624
Val Asp Leu Ile Asn Leu Arg Ser Ile Ser Val Asp Ala Glu Asn Asn
195 200 205
gtt gct tgg gtt caa tct ggt gct act att ggt gag ttg tac tac aga 672
Val Ala Trp Val Gln Ser Gly Ala Thr Ile Gly Glu Leu Tyr Tyr Arg
210 215 220
att gcc gaa aag tct aag acc ttg ggt ttt cca gct ggt gtt tgt cca 720
Ile Ala Glu Lys Ser Lys Thr Leu Gly Phe Pro Ala Gly Val Cys Pro
225 230 235 240
act gtt ggt gtt ggt ggt cac ttt tct ggt ggt ggt tat ggt ttg atg 768
Thr Val Gly Val Gly Gly His Phe Ser Gly Gly Gly Tyr Gly Leu Met
245 250 255
ttg aga aaa ttt ggt ttg gcc gct gat aac gtc att gat gct tac ttc 816
Leu Arg Lys Phe Gly Leu Ala Ala Asp Asn Val Ile Asp Ala Tyr Phe
260 265 270
att gat gtc aac ggt aag ctg cat gac aga aaa tct atg ggt gaa gat 864
Ile Asp Val Asn Gly Lys Leu His Asp Arg Lys Ser Met Gly Glu Asp
275 280 285
gtt ttc tgg gct att aga ggt ggt ggt ggc gct tct ttt ggt ata gtt 912
Val Phe Trp Ala Ile Arg Gly Gly Gly Gly Ala Ser Phe Gly Ile Val
290 295 300
ttg gct tgg aag atc aag ttg ttg cca gtt cca tct atc gtt act gtt 960
Leu Ala Trp Lys Ile Lys Leu Leu Pro Val Pro Ser Ile Val Thr Val
305 310 315 320
ttc act att acc aga acc ttg gaa caa aac gcc acc gat ttg att cat 1008
Phe Thr Ile Thr Arg Thr Leu Glu Gln Asn Ala Thr Asp Leu Ile His
325 330 335
aga tgg caa tac gtt tct tct aag cag gat gac aag ttg ttc atc aga 1056
Arg Trp Gln Tyr Val Ser Ser Lys Gln Asp Asp Lys Leu Phe Ile Arg
340 345 350
att atc ttg acc ggt atc aac acc acc aat tca caa cat ggt aac aag 1104
Ile Ile Leu Thr Gly Ile Asn Thr Thr Asn Ser Gln His Gly Asn Lys
355 360 365
aga act att gaa gct gcc ttc aac tct ttg ttc ttg ggt aag att gaa 1152
Arg Thr Ile Glu Ala Ala Phe Asn Ser Leu Phe Leu Gly Lys Ile Glu
370 375 380
gag ttg gtc cca att atg gaa aag act ttc cca gaa ttg ggt ttg acc 1200
Glu Leu Val Pro Ile Met Glu Lys Thr Phe Pro Glu Leu Gly Leu Thr
385 390 395 400
aaa gaa gat tgc att gag atg tcc tgg att gag tct gtc ttg tac ttt 1248
Lys Glu Asp Cys Ile Glu Met Ser Trp Ile Glu Ser Val Leu Tyr Phe
405 410 415
gct ggt ttt tct aga ggt caa cca ttg aac gtt ttg ttg gat aga agg 1296
Ala Gly Phe Ser Arg Gly Gln Pro Leu Asn Val Leu Leu Asp Arg Arg
420 425 430
cca ttg act cca aag aga ttt ttc aag gct aag tcc gat tac gtc aac 1344
Pro Leu Thr Pro Lys Arg Phe Phe Lys Ala Lys Ser Asp Tyr Val Asn
435 440 445
gaa cct att cca aaa gct ggt ttg gaa ggt att tgg gag ttg ttc gat 1392
Glu Pro Ile Pro Lys Ala Gly Leu Glu Gly Ile Trp Glu Leu Phe Asp
450 455 460
gaa gaa gaa gct gaa gct gct gtt ttg att ttg tct cca tac ggt ggt 1440
Glu Glu Glu Ala Glu Ala Ala Val Leu Ile Leu Ser Pro Tyr Gly Gly
465 470 475 480
atc atg gac aag att tcc gaa tcc gaa att cca ttt cca cat aga gct 1488
Ile Met Asp Lys Ile Ser Glu Ser Glu Ile Pro Phe Pro His Arg Ala
485 490 495
ggc aac ctg tac aaa atc caa cat ttg gtt tac tgg gac gaa gaa ggt 1536
Gly Asn Leu Tyr Lys Ile Gln His Leu Val Tyr Trp Asp Glu Glu Gly
500 505 510
gaa gat att gct aag aga cat atc aac tgg atc aga aag ctg tac tct 1584
Glu Asp Ile Ala Lys Arg His Ile Asn Trp Ile Arg Lys Leu Tyr Ser
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tac atg gct cca ttc gtt tct aaa aat cca aga gct gcc tac atc aac 1632
Tyr Met Ala Pro Phe Val Ser Lys Asn Pro Arg Ala Ala Tyr Ile Asn
530 535 540
tac aga gat ttg gat att ggc acc aac gaa aag aac ggt aga aac act 1680
Tyr Arg Asp Leu Asp Ile Gly Thr Asn Glu Lys Asn Gly Arg Asn Thr
545 550 555 560
tct tac aac gaa gct tct gtt tgg ggt gtt aag tac ttt aag ggt aac 1728
Ser Tyr Asn Glu Ala Ser Val Trp Gly Val Lys Tyr Phe Lys Gly Asn
565 570 575
ttc aag agg ctg gtt tct gtt aag aca aag gtt gac cca tcc aat ttc 1776
Phe Lys Arg Leu Val Ser Val Lys Thr Lys Val Asp Pro Ser Asn Phe
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ttc aag aac gaa caa tcc att cca tcc ttg aaa cca tgg tgg aac aag 1824
Phe Lys Asn Glu Gln Ser Ile Pro Ser Leu Lys Pro Trp Trp Asn Lys
595 600 605
aga ggt tat tgg tca cat cca caa ttt gaa aag 1857
Arg Gly Tyr Trp Ser His Pro Gln Phe Glu Lys
610 615
<210> 135
<211> 619
<212> PRT
<213> 人工序列
<220>
<223> 合成构建物
<400> 135
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser
1 5 10 15
Ala Leu Ala Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln
20 25 30
Ile Pro Ala Glu Ala Val Ile Gly Tyr Ser Asp Leu Glu Gly Asp Phe
35 40 45
Asp Val Ala Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu
50 55 60
Phe Ile Asn Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val
65 70 75 80
Ser Leu Glu Lys Arg Glu Ala Glu Ala Met Ala His Asn His Glu Ser
85 90 95
Phe Leu His Cys Leu Ser His His Ser Pro Asn Thr Ser Ser Ile Ser
100 105 110
Lys Ile Ile Tyr Thr Pro Thr Asn Pro Ser Tyr Ser Ser Val Leu Asn
115 120 125
Phe Ser Ile His Asn Leu Arg Phe Thr Ser Pro Lys Thr Pro Lys Pro
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His Ile Ile Val Thr Pro Phe Leu Val Ser Gln Ile Gln Ala Ser Ile
145 150 155 160
Ile Cys Ala Lys Lys Thr Gly Phe Gln Ile Arg Thr Arg Ser Gly Gly
165 170 175
His Asp Tyr Glu Gly Leu Ser Tyr Val Ser Asp Val Ser Phe Val Val
180 185 190
Val Asp Leu Ile Asn Leu Arg Ser Ile Ser Val Asp Ala Glu Asn Asn
195 200 205
Val Ala Trp Val Gln Ser Gly Ala Thr Ile Gly Glu Leu Tyr Tyr Arg
210 215 220
Ile Ala Glu Lys Ser Lys Thr Leu Gly Phe Pro Ala Gly Val Cys Pro
225 230 235 240
Thr Val Gly Val Gly Gly His Phe Ser Gly Gly Gly Tyr Gly Leu Met
245 250 255
Leu Arg Lys Phe Gly Leu Ala Ala Asp Asn Val Ile Asp Ala Tyr Phe
260 265 270
Ile Asp Val Asn Gly Lys Leu His Asp Arg Lys Ser Met Gly Glu Asp
275 280 285
Val Phe Trp Ala Ile Arg Gly Gly Gly Gly Ala Ser Phe Gly Ile Val
290 295 300
Leu Ala Trp Lys Ile Lys Leu Leu Pro Val Pro Ser Ile Val Thr Val
305 310 315 320
Phe Thr Ile Thr Arg Thr Leu Glu Gln Asn Ala Thr Asp Leu Ile His
325 330 335
Arg Trp Gln Tyr Val Ser Ser Lys Gln Asp Asp Lys Leu Phe Ile Arg
340 345 350
Ile Ile Leu Thr Gly Ile Asn Thr Thr Asn Ser Gln His Gly Asn Lys
355 360 365
Arg Thr Ile Glu Ala Ala Phe Asn Ser Leu Phe Leu Gly Lys Ile Glu
370 375 380
Glu Leu Val Pro Ile Met Glu Lys Thr Phe Pro Glu Leu Gly Leu Thr
385 390 395 400
Lys Glu Asp Cys Ile Glu Met Ser Trp Ile Glu Ser Val Leu Tyr Phe
405 410 415
Ala Gly Phe Ser Arg Gly Gln Pro Leu Asn Val Leu Leu Asp Arg Arg
420 425 430
Pro Leu Thr Pro Lys Arg Phe Phe Lys Ala Lys Ser Asp Tyr Val Asn
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Glu Pro Ile Pro Lys Ala Gly Leu Glu Gly Ile Trp Glu Leu Phe Asp
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Glu Glu Glu Ala Glu Ala Ala Val Leu Ile Leu Ser Pro Tyr Gly Gly
465 470 475 480
Ile Met Asp Lys Ile Ser Glu Ser Glu Ile Pro Phe Pro His Arg Ala
485 490 495
Gly Asn Leu Tyr Lys Ile Gln His Leu Val Tyr Trp Asp Glu Glu Gly
500 505 510
Glu Asp Ile Ala Lys Arg His Ile Asn Trp Ile Arg Lys Leu Tyr Ser
515 520 525
Tyr Met Ala Pro Phe Val Ser Lys Asn Pro Arg Ala Ala Tyr Ile Asn
530 535 540
Tyr Arg Asp Leu Asp Ile Gly Thr Asn Glu Lys Asn Gly Arg Asn Thr
545 550 555 560
Ser Tyr Asn Glu Ala Ser Val Trp Gly Val Lys Tyr Phe Lys Gly Asn
565 570 575
Phe Lys Arg Leu Val Ser Val Lys Thr Lys Val Asp Pro Ser Asn Phe
580 585 590
Phe Lys Asn Glu Gln Ser Ile Pro Ser Leu Lys Pro Trp Trp Asn Lys
595 600 605
Arg Gly Tyr Trp Ser His Pro Gln Phe Glu Lys
610 615

Claims (14)

1.生产一种或多种大麻素的方法，所述方法包括使大麻萜酚酸(CBGA)与大麻素合酶直系同源物接触，其中所述直系同源物来自除大麻以外的生物体。
2.根据权利要求1所述的方法，其中所述大麻素合酶直系同源物来自橙子、甘蓝型油菜、烟草、陆地棉、水稻亚种籼稻、水稻亚种粳稻、拟南芥亚种lyrata、类芽孢杆菌Aloe-11、链霉菌ipomoeae 91-03、芜菁、桃、枯草芽孢杆菌168、拟南芥、罂粟或寄生疫霉P1569。
3.根据权利要求2所述的方法，其中所述大麻素合酶直系同源物选自表1和2中所示的直系同源物。
4.根据权利要求3所述的方法，其中所述大麻素合酶直系同源物包括选自SEQ ID NO:7、16、23、31、37、47、57、67、77、87、97、107、117和127的氨基酸序列，或与SEQ ID NO:7、16、23、31、37、47、57、67、77、87、97、107和127具有70％或更高同一性的序列。
5.根据权利要求1所述的方法，其中所生产的大麻素具有358.5g/mol、374.5g/mol或330.5g/mol的配方重量。
6.根据权利要求5所述的方法，其中所生产的大麻素包括六氢次大麻呋酚酸和大麻酚素。
7.根据权利要求6所述的方法，其中所生产的大麻素不含配方重量为358.5g/mol的任何大麻素，并且所述大麻素合酶直系同源物包括SEQ ID NO:67、77、97或127的氨基酸序列或与SEQ ID NO:67、77、97或127具有70％或更高同一性的序列。
8.根据权利要求1所述的方法，其中，所述大麻素合酶直系同源物是在酿酒酵母、解脂耶氏酵母、马克斯克鲁维酵母或毕赤酵母中产生的重组酶。
9.根据权利要求8所述的方法，其中所述大麻素合酶直系同源物选自表1和2中所示的直系同源物。
10.根据权利要求9所述的方法，其中所述大麻素合酶直系同源物包括选自SEQ ID NO:7、16、23、31、37、47、57、67、77、87、97、107、117和127的氨基酸序列，或与SEQ ID NO:7、16、23、31、37、47、57、67、77、87、97、107、117和127具有70％或更高同一性的序列。
11.一种酿酒酵母或毕赤酵母的重组细胞，在其基因组中包含编码大麻素合酶直系同源物的核酸，其中大麻素合酶直系同源物来自橙子、甘蓝型油菜、烟草、陆地棉、水稻亚种籼稻、水稻亚种粳稻、拟南芥亚种lyrata、类芽孢杆菌Aloe-11、链霉菌ipomoeae 91-03、芜菁、桃、枯草芽孢杆菌168、拟南芥、罂粟或寄生疫霉P1569，所述大麻素合酶直系同源物在重组细胞中以活性形式表达。
12.根据权利要求11所述的重组细胞，其中所述核酸编码选自表1和表2所示的大麻素合酶直系同源物。
13.根据权利要求12所述的重组细胞，其中所述核酸编码大麻素合酶直系同源物，所述直系同源物包括选自SEQ ID NO:7、16、23、31、37、47、57、67、77、87、97、107、117和127的氨基酸序列，或与SEQ ID NO:7、16、23、31、37、47、57、67、77、87、97、107、117和127具有70％或更高同一性的序列。
14.根据权利要求13所述的重组细胞，其中所述核酸编码大麻素合酶直系同源物，所述直系同源物包括SEQ ID NO:67、77、97或127的氨基酸序列或与SEQ ID NO:67、77、97或127具有70％或更高同一性的序列。
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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140057251A1 (en) * 2011-08-18 2014-02-27 Medicinal Genomics Corporation Cannabis Genomes and Uses Thereof
CN105555265A (zh) * 2013-02-28 2016-05-04 全谱实验室有限公司 大麻素的生物合成
WO2016189384A1 (en) * 2015-05-28 2016-12-01 Tweed Inc. Cannabis plants having modified expression of thca synthase
WO2017139496A1 (en) * 2016-02-09 2017-08-17 Cevolva Biotech, Inc. Microbial engineering for the production of cannabinoids and cannabinoid precursors
WO2019046941A1 (en) * 2017-09-05 2019-03-14 Inmed Pharmaceuticals Inc. METABOLIC ENGINEERING OF E. COLI FOR BIOSYNTHESIS OF CANNABINOID PRODUCTS

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2898272T3 (es) * 2017-04-27 2022-03-04 Univ California Microorganismos y métodos para producir cannabinoides y derivados de cannabinoides
WO2019190945A1 (en) * 2018-03-29 2019-10-03 William Marsh Rice University Biosynthesis of olivetolic acid

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140057251A1 (en) * 2011-08-18 2014-02-27 Medicinal Genomics Corporation Cannabis Genomes and Uses Thereof
CN105555265A (zh) * 2013-02-28 2016-05-04 全谱实验室有限公司 大麻素的生物合成
WO2016189384A1 (en) * 2015-05-28 2016-12-01 Tweed Inc. Cannabis plants having modified expression of thca synthase
WO2017139496A1 (en) * 2016-02-09 2017-08-17 Cevolva Biotech, Inc. Microbial engineering for the production of cannabinoids and cannabinoid precursors
WO2019046941A1 (en) * 2017-09-05 2019-03-14 Inmed Pharmaceuticals Inc. METABOLIC ENGINEERING OF E. COLI FOR BIOSYNTHESIS OF CANNABINOID PRODUCTS

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
ARYAL N等: "Distribution of cannabinoid synthase genes in non-Cannabis organisms", JOURNAL OF CANNABIS RESEARCH, vol. 1, no. 1, pages 4, XP093089111, DOI: 10.1186/s42238-019-0008-7 *
BRASSICA RAPA GENOME SEQUENCING PROJECT CONSORTIUM: "M4DIE5_BRARP，BBE domain-containing protein", UNIPROT, 18 September 2019 (2019-09-18), pages 1 - 2 *
彭思琪: "大麻二酚酸合成毕赤酵母菌株的构建及优化", 中国优秀硕士学位论文全文数据库工程科技Ⅰ辑, no. 10, pages 018 - 64 *

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