CN114600889B - Composite herbicide containing 2-methyl-4-chloro and application thereof in inhibiting weed growth - Google Patents
Composite herbicide containing 2-methyl-4-chloro and application thereof in inhibiting weed growth Download PDFInfo
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- CN114600889B CN114600889B CN202210371945.9A CN202210371945A CN114600889B CN 114600889 B CN114600889 B CN 114600889B CN 202210371945 A CN202210371945 A CN 202210371945A CN 114600889 B CN114600889 B CN 114600889B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N39/00—Biocides, pest repellants or attractants, or plant growth regulators containing aryloxy- or arylthio-aliphatic or cycloaliphatic compounds, containing the group or, e.g. phenoxyethylamine, phenylthio-acetonitrile, phenoxyacetone
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- A01N39/04—Aryloxy-acetic acids; Derivatives thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
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Abstract
The invention discloses a compound herbicide containing 2-methyl-4-chloro and application thereof in inhibiting weed growth, wherein the herbicide is 2-methyl-4-chloro, and the herbicide synergist is o-phthalaldehyde or 2, 3-dihydroxybenzaldehyde. The two compounds of the o-phthalaldehyde or the 2, 3-dihydroxybenzaldehyde have remarkable synergistic effect on the herbicide 2-methyl-4-chloro, the dosage of the herbicide 2-methyl-4-chloro can be reduced, the influence of the herbicide on the environment is reduced, the risk of drug resistance of weeds to the 2-methyl-4-chloro is reduced, and the cost of the medicament can be reduced. The compound herbicide has the advantages of simple formula, low cost and excellent weeding effect, can be widely used for inhibiting the growth of various weeds, and has a wide application range.
Description
Technical Field
The invention relates to the technical field of herbicides, in particular to a compound herbicide containing 2-methyl-4-chloro and application thereof in inhibiting weed growth.
Background
Since the growth of weeds greatly affects the normal growth of crops, the yield and quality of agricultural products are easily reduced, and even the particles are not harvested when serious. Accordingly, there has been a constant interest in herbicidal technology in agricultural production. The traditional manual weeding method is low in efficiency and high in labor cost. With the rapid development of technology, chemical herbicides and the advantages of high weeding efficiency and convenient use thereof have become one of the indispensable technical measures in modern agricultural production.
A variety of different chemical herbicides have been developed, but the herbicidal performance of a single herbicide is limited, and herbicide compositions used by mixing a plurality of herbicides have been developed gradually to improve the herbicidal effect of the herbicides. The mixed application of the herbicide is to mix the herbicides with different action mechanisms so as to achieve the purposes of improving the weeding effect, delaying the formation of herbicide resistance of weeds to the herbicide, expanding the weed killing spectrum and the like; the synergistic agent is added by adding a compound with synergistic effect into the herbicide preparation to improve the weeding effect of the herbicide.
2-methyl-4-chloro (MCPA-Na; 2-methyl-4-chlorophenoxy acetic acid) is a phenoxyacetic acid selective hormone herbicide, is widely used for preventing and killing various annual and perennial broadleaf weeds and part of sedge weeds in crop fields such as rice, wheat and the like and after non-cultivated land seedlings, and has the advantages of high efficiency, low toxicity, safety and the like, and is widely used.
The weeding performance of the 2-methyl-4-chloro is further improved, and the method has important significance for the healthy development of herbicide industry in China and the guarantee of grain safety in China. However, there is no herbicidal synergist having a significant synergistic effect on 2-methyl-4-chloro.
Accordingly, there is a need for further improvements in the art.
Disclosure of Invention
In view of the above problems, the present invention provides a compound herbicide containing 2-methyl-4-chloro and application thereof, wherein the compound herbicide uses phthalaldehyde and 2, 3-dihydroxybenzaldehyde as herbicide synergists, and the synergists effectively improve the herbicidal activity of the 2-methyl-4-chloro through synergistic cooperation.
The applicant discovers through a large number of experiments and researches that two compounds of phthalic dicarboxaldehyde or 2, 3-dihydroxybenzaldehyde are used independently, have good herbicidal activity, can effectively inhibit the growth of the rhizomes of amaranthus retroflexus, eclipta prostrata, barnyard grass, crabgrass, goosegrass and nutgrass galingale seedlings, have different degrees of herbicidal effects on the amaranthus retroflexus under the condition of potting soil treatment, and have higher synergistic activity on 2-methyl-4-chloro when used as a herbicide synergist. Therefore, the o-phthalaldehyde or the 2, 3-dihydroxybenzaldehyde can be used as a herbicide synergist to be matched with the 2-methyl-4-chloro, so that the herbicide cost can be reduced while the herbicide activity is improved.
For this purpose, the invention provides the following technical scheme:
in a first aspect, the present invention provides a composite herbicide comprising 2-methyl-4-chloro, which comprises a herbicide comprising 2-methyl-4-chloro as a major ingredient and a herbicide synergist.
On the basis, in order to prolong the duration of the herbicide synergist, a carrier, an antioxidant or other auxiliary agents can be added into the herbicide synergist.
Further, in the compound herbicide containing 2-methyl-4-chloro, the herbicide synergist is phthalic dicarboxaldehyde.
Preferably, the concentration of the phthalic aldehyde is 1.5625mg/L to 12.5mg/L, and the concentration of the 2-methyl-4-chloro is 0.025mg/L to 25mg/L.
Experiments prove that the phthalic dicarboxaldehyde has a synergistic effect on 2-methyl-4-chloro. Experiments prove that the concentrations of 0mg/L, 1.5625mg/L, 3.125mg/L, 6.25mg/L and 12.5mg/L of the phthalic dicarboxaldehyde are mutually mixed with the concentrations of 0mg/L, 0.025mg/L, 0.25mg/L, 2.5mg/L and 25mg/L of the 2-methyl-4-chloro, and the good synergistic effect is shown.
Preferably, in the composite herbicide, the mass ratio of 2-methyl-4-chloro to phthalic aldehyde is (8-20): 1. In the range, the synergistic effect of the phthalic dicarboxaldehyde on 2-methyl-4-chloro is better. Preferably, the mass ratio of the 2-methyl-4-chloro to the phthalic aldehyde is 16:1; under the condition of the proportion, the synergistic effect is obvious, and the co-toxicity coefficients of the embryonic axis and the radicle of the amaranthus retroflexus seedling are 173.1 and 290.3 respectively, which shows that the two compounds have good synergistic effect.
Further preferably, the mass percentage concentration of 2-methyl-4-chloro and phthalic aldehyde is 94.1% and 5.9%, respectively. Under the condition of the concentration, the weeding effect of the composite herbicide is optimal.
Further, in the composite herbicide containing 2-methyl-4-chloro provided by the invention, the herbicide synergist is 2, 3-dihydroxybenzaldehyde.
Preferably, the concentration of 2, 3-dihydroxybenzaldehyde is 3.125mg/L to 25mg/L, and the concentration of 2-methyl-4-chloro is 0.025mg/L to 25mg/L.
Experiments prove that the five concentrations of 0, 3, 125mg/L, 6.25mg/L, 12.5mg/L and 25mg/L of 2, 3-dihydroxybenzaldehyde are mutually mixed with the five concentrations of 0mg/L, 0.025mg/L, 0.25mg/L, 2.5mg/L and 25mg/L of 2-methyl-4-chloro, and the good synergistic effect is shown.
Preferably, in the composite herbicide, the mass ratio of the 2-methyl-4-chloro to the 2, 3-dihydroxybenzaldehyde is (1.5-50): 1. In the range, the synergistic effect of the phthalic dicarboxaldehyde on 2-methyl-4-chloro is better. Further preferably, when the mass ratio of the 2-methyl-4-chloro to the 2, 3-dihydroxybenzaldehyde is 14:1, the synergistic effect of the mixture is remarkable, and the co-toxicity coefficients of the mixture on the hypocotyl and the radicle of the amaranthus retroflexus seedling are 253.4 and 300.5 respectively.
Further preferably, the mass percentage concentrations of 2-methyl-4-chloro, 2, 3-dihydroxybenzaldehyde are 93.3% and 6.7%, respectively. Under the condition of the concentration, the weeding effect of the compound herbicide is optimal.
In a second aspect, the present invention also provides the use of the above-described composite herbicide for inhibiting the growth of weeds, wherein the weeds are amaranthus retroflexus, eclipta prostrata, barnyard grass, crabgrass, gooseberry, nutgrass galingale rhizome and the like.
The invention has the following beneficial effects:
1. the o-phthalaldehyde and the 2, 3-dihydroxybenzaldehyde are found to have remarkable synergistic effect on the herbicide 2-methyl-4-chloro which is commonly used at present for the first time. The compound herbicide can be prepared by mixing, has obvious inhibition activity on the growth of weed seedling radicle (seed root) and hypocotyl (coleoptile) of amaranthus retroflexus, eclipta, crabgrass, barnyard grass, goosegrass, nutgrass galingale and the like, and has excellent weeding effect.
2. According to the invention, through optimizing the mixing concentration and proportion of the compounds, the synergistic effect of the o-phthalaldehyde or the 2, 3-dihydroxybenzaldehyde on the 2-methyl-4-chloro can be maximized, and the use of the synergistic agent can obviously reduce the dosage of the herbicide 2-methyl-4-chloro, so that the influence of the herbicide on the environment is reduced, and the risk of drug resistance of weeds on the 2-methyl-4-chloro is reduced; meanwhile, the price of the phthalic dicarboxaldehyde and the 2, 3-dihydroxybenzaldehyde is far lower than that of 2-methyl-4-chloro, so that the cost of the medicament can be reduced by mixing.
Drawings
FIG. 1 shows the control effect of soil treatment of phthalic dicarboxaldehyde and 2, 3-dihydroxybenzaldehyde on amaranthus retroflexus under potting conditions; 3, 6-dichloropyridine acid was used as a control agent.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to fall within the scope of the invention. In the present invention, the equipment, materials, etc. used are commercially available or commonly used in the art, unless otherwise specified. The methods in the following examples are conventional in the art unless otherwise specified.
Experimental materials and Activity determination methods
1. Recipient plant seed
The following examples use 6 farmland-familiar weeds, amaranthus retroflexus (Amaranthus retroflexus l.), eclipta, barnyard grass (echihhloerus galli), crabgrass (digitariasaanguina), eleusina (eleusindica), nutgrass (cyperus rotundus) as recipient plants. Seeds were harvested from green island city, i.e., the Gao Cheng farm in the ink district, and from a wasteland near the green island agricultural university where no herbicide was used. Before use, the plant seeds are stored in a seed cabinet at 6 ℃ for standby.
2. Test agent
98% phthalic dicarboxaldehyde (Shanghai Miclin Biochemical technology Co., ltd.)
2, 3-dihydroxybenzaldehyde (Shanghai Ala Biochemical technology Co., ltd.)
98% of 2-methyl-4-chloro (Heilier pharmaceutical Co., ltd.)
99% of 3, 6-dichloropicolinic acid (Ark Pharm)
3. Medicament treatment
Determining herbicidal activity of phthalaldehyde and 2, 3-dihydroxybenzaldehyde
Different concentration series were set according to the sensitivity of each weed, 5 concentrations per series.
Equivalent effect line measurement
Five series concentrations of phthalic dicarboxaldehyde or 2, 3-dihydroxybenzaldehyde and five series concentrations of 2-methyl-4-chloro are mixed with each other, and 25 mixed concentration treatments are set. Before preparation, three reagents are dissolved by dimethyl sulfoxide to prepare high-concentration mother solution for later use. Wherein, the serial concentration of the phthalic dicarboxaldehyde is 0mg/L, 1.5625mg/L, 3.125mg/L, 6.25mg/L and 12.5mg/L; the serial concentrations of 2, 3-dihydroxybenzaldehyde are 0mg/L, 3.125mg/L, 6.25mg/L, 12.5mg/L and 25mg/L; the serial concentrations of 2-methyl-4-chloro were 0mg/L, 0.025mg/L, 0.25mg/L, 2.5mg/L and 25mg/L.
Determination of co-toxicity coefficient
The method needs to separately determine the EC of each single agent and mixed agent of the mixed components 50 And calculating the co-toxicity coefficient by combining the percentage of each single agent in the mixture.
The mixing ratio of 2-methyl-4-chloro and phthalic aldehyde was set to be 16:1 (94.1% and 5.9% by weight of the two, respectively). Wherein, the series concentration of the 2-methyl-4-chloride independent treatment is 0.0085mg/L, 0.085mg/L, 0.85mg/L and 8.5mg/L, the series concentration of the phthalic dicarboxaldehyde independent treatment is 0mg/L, 1.5625mg/L, 3.125mg/L, 6.25mg/L and 12.5mg/L, and the series concentration of the mixture treatment is 0mg/L, 0.0085mg/L, 0.085mg/L, 0.85mg/L and 8.5mg/L.
The mixing ratio of 2-methyl-4-chloro and 2, 3-dihydroxybenzaldehyde was set to be 14:1 (93.3% and 6.7% by weight respectively). The concentrations of the series of 2-methyl-4-chloro treatments were 0mg/L, 0.05mg/L, 0.5mg/L, 5mg/L and 50mg/L, and the concentrations of the series of 2, 3-dihydroxybenzaldehyde treatments were 0mg/L, 3.125mg/L, 6.25mg/L, 12.5mg/L and 25.0mg, and the concentrations of the series of the mixture treatments were 0mg/L, 0.05mg/L, 0.5mg/L, 5mg/L and 50mg/L.
4. Biological Activity assay
Preparation method of agar
(1) Treatment of test plant seeds
Soaking the seeds of Amaranthus retroflexus in 2% sodium hypochlorite solution for 10-15min, washing with distilled water for 5-6 times, and soaking in water for 6-8 hr. Spreading two layers of kitchen paper in a square tray which is cleaned and sterilized by 75% alcohol, wetting with distilled water, flushing the water-absorbed amaranthus retroflexus seeds with distilled water for several times, uniformly placing on the kitchen paper, covering, placing in a constant temperature climatic incubator at 25 ℃ for germination acceleration, and keeping until the radicle (seed root) of the plant seedling grows to 3-5mm for later use.
(2) Preparation of agar containing medicament
The mother solution of phthalaldehyde (or 2, 3-dihydroxybenzaldehyde) and 2-methyl-4-chloro is absorbed into 0.5% agar solution to prepare 100ml agar matrixes with different mixed concentrations, and then the agar matrixes are evenly distributed in three small beakers, and the agar matrixes are placed for condensation. Agar medium containing DMSO alone was used as a blank. The DMSO content in each treatment was kept consistent, and was 1% in the medium effect line method and 0.5% in the co-toxicity coefficient method.
(3) Transplanting of test plant seedlings
Selecting plant sprouting seeds with basically consistent root length, firstly inserting 5 small holes on the surface of a solidified agar culture medium by using pointed forceps, then lightly inserting radicle of the seeds, repeating 5 grains per beaker for 3 times, placing in a paper box for shading, and culturing for 3-4d in a plant growth box. The growth chamber was set up with continuous circulation of light for 14h (25 ℃) and darkness for 10h (20 ℃) with a relative humidity of 60%.
(4) Result measurement and data analysis
The treated seedlings were removed from the beaker, the lengths of radicle and coleoptile were measured with a vernier caliper, and the growth was calculated. The data were analyzed using Excel software, the rate of inhibition and standard error for radicle and hypocotyl were calculated, and the effective medium concentration (EC 50) for each treatment was analyzed using SPSS software. The main calculation formula is as follows:
growth = radicle (or hypocotyl) length after treatment-radicle (or hypocotyl) length before treatment
Inhibition ratio (%) = (control growth amount-treatment growth amount)/control growth amount×100
Wherein: a is a medicament A; b is a medicament B; m is a mixture of the medicaments A and B; p (P) A And P B The percentages of the A medicament and the B medicament in the mixture are respectively.
Potted plant method (soil treatment)
And (5) airing and breaking field soil, and sieving the field soil with a 20-mesh sieve. Pricking 4-5 small holes at the bottom of a disposable plastic cup, loading the sieved soil to a height of 0.5cm from the cup mouth, placing the soil in a tray filled with tap water, inoculating germinated seeds after water absorption and complete penetration into surface soil,5 grains per cup, and then covering fine soil with the thickness of 0.5cm until the fine soil is leveled with the cup opening. The medicament spraying is carried out by a walking type spray tower. Taking aqueous solution containing solvent (DSMO) and Tween 20 at the same concentration as blank control, and the dosage is 450L/hm 2 . Each treatment was repeated three times. Seedlings were observed daily for emergence and growth after application. And about three weeks after treatment, when the difference of growth vigor is obvious, the number of surviving plants is investigated, the plant height and fresh weight of other plants are measured, and the plant height control effect and fresh weight control effect are calculated.
Example determination of herbicidal Activity of phthalic dicarboxaldehyde, 2, 3-dihydroxybenzaldehyde
1. Agar assay
(1) The experimental method comprises the following steps:
the inhibition effect of phthalic aldehyde and 2, 3-dihydroxybenzaldehyde on growth of radicle (seed root) and hypocotyl (coleoptile) of six main farmland weed seedlings was measured by the agar method.
(2) Experimental results and analysis:
TABLE 1 effective neutral concentrations of phthalic dicarboxaldehyde and 2, 3-dihydroxybenzaldehyde (EC) to inhibit weed seedling growth 50 ，mg/L)
From the results shown in Table 1, it was found that phthalic aldehyde and 2, 3-dihydroxybenzaldehyde had remarkable inhibitory activity against the growth of six weeds (particularly radicle (seed root)) tested, so that the inhibitory activity against amaranthus retroflexus, eclipta, barnyard grass and crabgrass was the highest, the inhibitory activity against the growth of nutgrass flatsedge and goosegrass, particularly both coleoptile, was relatively low, and similar inhibitory trends were exhibited between the two agents. Wherein, the inhibition of six weed hypocotyls (coleoptile) by phthalic dicarboxaldehyde EC 50 From low to high, the dosage is respectively 4.039mg/L (amaranthus retroflexus), 4.714mg/L (eclipta alba), 4.831mg/L (crabgrass), 6.729mg/L (barnyard grass) and 23.898mg/L (Eleutherococcus) and 31.262mg/L (Cyperus rotundus), inhibiting EC on their radicle (seed root) 50 From low to high, 0.805mg/L (eclipta), 0.925mg/L (amaranthus retroflexus), 2.050mg/L (barnyard grass), 3.048mg/L (goosegrass), 3.215mg/L (crabgrass) and 3.570mg/L (nutgrass galingale rhizome) are respectively arranged in sequence. Inhibition of six weed hypocotyls (coleoptile) by 2, 3-dihydroxybenzaldehyde EC 50 From low to high, 4.836mg/L (amaranthus retroflexus), 6.317mg/L (eclipta), 7.831mg/L (crabgrass), 9.234mg/L (barnyard grass), 25.736mg/L (goosegrass) and 33.248mg/L (nutgrass) respectively inhibit EC of radicle (seed root) thereof 50 From low to high, the dosage is respectively 1.024mg/L (amaranthus retroflexus), 1.805mg/L (eclipta alba), 2.054mg/L (barnyard grass), 3.332mg/L (goosegrass), 4.215mg/L (nutgrass) and 6.451mg/L (goosegrass).
2. Potted plant test
(1) The experimental method comprises the following steps: the control effect of the two agents on the most sensitive amaranthus retroflexus is further determined by a potting method through soil treatment.
(2) Experimental results and analysis
TABLE 2 control effects of soil treatment of O-phthalaldehyde and 2, 3-dihydroxybenzaldehyde on Amaranthus retroflexus under potting conditions
The results in Table 2 and FIG. 1 show that the control effect of the phthalic dicarboxaldehyde on amaranth is basically equivalent to that of the common herbicide 3, 6-dichloropicolinic acid, and the plant height and fresh weight control effect are only 50.2% and 45.5% respectively at 200mg/L, and the control effect is as high as 100% at the concentration of 1000 mg/L-5000 mg/L.
While the effect of the 2, 3-dihydroxybenzaldehyde soil treatment on the amaranthus retroflexus is lower than that of the o-phthalaldehyde, the preventing and removing effect of the 2, 3-dihydroxybenzaldehyde soil treatment on the amaranthus retroflexus cannot be well reflected under the soil treatment condition, but the inhibiting effect of the 2, 3-dihydroxybenzaldehyde is further increased along with the increase of the concentration of the 2, 3-dihydroxybenzaldehyde; the plant height control effect and fresh weight control effect under 5000mg/L treatment respectively reach 73.6% and 85.5%, which shows that the agent has higher control effect on the growth of amaranthus retroflexus seedlings under the soil treatment condition, and the required treatment concentration is relatively higher. The reason for this result may be related to the fact that the adsorption of 2, 3-dihydroxybenzaldehyde is higher than that of phthalic dicarboxaldehyde by organic matters in the soil, etc., resulting in the decrease of the effectiveness of 2, 3-dihydroxybenzaldehyde. As the agar method is used for measuring the toxicity of the medicament to the seedlings of the tested plants, the influence condition is single, and the potting method is used for combining various factors such as soil, and the difference of the effective concentration of the two factors is normal.
Example herbicidal effect of Di-o-phthalaldehyde and 2-methyl-4-chloro
1. The experimental method comprises the following steps:
(1) Five concentrations of the respective agents of phthalic dicarboxaldehyde (0 mg/L, 1.5625mg/L, 3.125mg/L, 6.25mg/L and 12.5 mg/L) and 2-methyl-4-chloro (0.025 mg/L, 0.25mg/L, 2.5mg/L and 25 mg/L) were respectively prepared, and were compounded with each other, and herbicidal effects of the compounded agents were measured.
(2) Preparing a composite herbicide: the mass ratio of 2-methyl-4-chloro to 2, 3-dihydroxybenzaldehyde was 16:1 (94.1% and 5.9% of the two agents by mass), and the respective ECs were obtained by treating the two agents respectively according to the series concentrations of the single agent and the mixed agent listed in the above agent treatment section 50 The value and thus the co-toxicity coefficient are calculated by the method described above.
2. Experimental results:
TABLE 3 Effect of o-phthalaldehyde and 2-methyl-4-chloro mixing on growth of amaranthus retroflexus seedling hypocotyls
Note that: the data in the table are inhibition (%) + -standard error.
TABLE 4 Effect of o-phthalaldehyde and 2-methyl-4-chloro mixing on growth of radicle of Amaranthus retroflexus seedling
Note that: the data in the table are inhibition (%) + -standard error.
(1) From the results in tables 3 and 4, it can be seen that: the inhibition rate of the growth of amaranthus retroflexus hypocotyl and radicle is improved along with the increase of the mixing concentration of the other medicament under each concentration of the o-phthalaldehyde or 2-methyl-4-chloro, and the o-phthalaldehyde and radicle show good synergistic effect.
Table 5 2 Co-toxicity coefficient of methyl 4 chloride and o-phthalaldehyde in 16:1 mixture for growth of radicle and hypocotyl of Amaranthus retroflexus seedling
(2) The results of the data in Table 3 and Table 4 and the effect lines are analyzed to determine that the synergistic ratio range is that the mass ratio of 2-methyl-4-chloro to phthalic aldehyde is between 8:1 and 20:1. Considering that 2 methyl 4 chloride is a herbicide commonly used in production, based on the thought that 2 methyl 4 chloride is taken as a main body of the mixed agent and phthalic aldehyde is taken as a synergistic agent, the proportion showing a good synergistic effect is selected, the mixed agent is mixed in a proportion of 16:1 by mass (94.1% and 5.9% by mass of the two agents respectively), the mixed agent is treated according to the serial concentrations of the single agent and the mixed agent listed in the agent treatment part to obtain respective EC50 values, and then the calculated co-toxicity coefficient calculation formula is adopted to measure that the co-toxicity coefficients of the two agents to the growth of the embryonic axis and the radicle of the anti-branch seedling are 173.1 and 290.3 respectively when the mixed agent and the o-branch seedling are mixed in the proportion, and the synergistic effect is obviously higher than 100, as shown in Table 5.
Example determination of herbicidal Activity of Tri-2, 3-dihydroxybenzaldehyde and 2-methyl-4-chloro
1. Experimental method
(1) Herbicidal effects were measured on five concentrations of 2, 3-dihydroxybenzaldehyde (0, 3.125mg/L, 6.25mg/L, 12.5mg/L and 25 mg/L) and 2-methyl-4-chloro (0 mg/L, 0.025mg/L, 0.25mg/L, 2.5mg/L and 25 mg/L) each.
(2) Preparing a composite herbicide: the mass ratio of 2-methyl-4-chloro to 2, 3-dihydroxybenzaldehyde was 14:1 (the parts by mass of the two medicaments were 93.3% and 6.7%, respectively), and the respective ECs were obtained by treating the respective mixtures according to the series concentrations of the single medicaments and the mixed medicaments listed in the medicament treatment section 50 The value and thus the co-toxicity coefficient are calculated by the method described above.
2. Experimental results and analysis:
TABLE 6 influence of 2, 3-dihydroxybenzaldehyde and 2-methyl-4-chloro on the growth of the hypocotyl of Amaranthus retroflexus seedlings
Note that: the data in the table are inhibition (%) + -standard error.
TABLE 7 influence of 2, 3-dihydroxybenzaldehyde on growth of radicle of Amaranthus retroflexus seedlings by mixing with 2-methyl-4-chloro
Note that: the data in the table are inhibition (%) + -standard error.
(1) From the results of tables 6 and 7, it was found that the inhibition rate of growth of amaranthus retroflexus hypocotyl and radicle at each concentration thereof was increased with the increase of the compounding concentration of the other agent, regardless of whether 2, 3-dihydroxybenzaldehyde or 2-methyl-4-chloro, and a good synergistic effect was exhibited.
Table 8 2 methyl 4 chloride and 2, 3-dihydroxybenzaldehyde were prepared according to 14:1 blending co-toxicity coefficients for the growth of radicle and hypocotyl of amaranthus retroflexus seedling
(2) The result analysis of the results in the table 6, the table 7 and the equal effect line shows that the synergistic ratio range is that the mass ratio of 2-methyl-4-chloro to 2, 3-dihydroxybenzaldehyde is 1.5:1-50:1. Considering that 2 methyl 4 chloride is a herbicide commonly used in production, the 2 methyl 4 chloride which shows better synergistic effect is selected based on the thought that the mixed agent takes 2 methyl 4 chloride as a main body and 2, 3-dihydroxybenzaldehyde as a synergistic agent: 2, 3-dihydroxybenzaldehyde=14:1 (93.3% and 6.7% of the two agents by mass fraction), and each EC was obtained by treating each agent in accordance with the series of concentrations of the single agent and the mixed agent listed in the above agent treatment section 50 The value is further calculated by the co-toxicity coefficient calculation formula, and the co-toxicity coefficients of the two for the growth of the embryonic axis and the radicle of the amaranthus retroflexus seedling are 253.4 and 300.5 respectively when the two are mixed, which are obviously higher than 100, and the synergistic effect is shown (the result is shown in table 8).
Claims (5)
1. A composite herbicide comprising 2-methyl-4-chloro, which comprises a herbicide and a herbicide synergist as main components, characterized in that the herbicide is 2-methyl-4-chloro; the herbicide synergist is phthalic dicarboxaldehyde or 2, 3-dihydroxybenzaldehyde; the mass ratio of the 2-methyl-4-chloro to the phthalic aldehyde is (8-20): 1; the mass ratio of the 2-methyl-4-chloro to the 2, 3-dihydroxybenzaldehyde is (1.5-50): 1.
2. The composite herbicide of claim 1, wherein the concentration of phthalic aldehyde is 1.5625mg/L to 12.5mg/L, the concentration of 2, 3-dihydroxybenzaldehyde is 3.125mg/L to 25mg/L, and the concentration of 2-methyl 4 chloride is 0.025mg/L to 25mg/L.
3. The composite herbicide according to claim 1, characterized in that the mass ratio of 2-methyl-4-chloro to phthalaldehyde is 16:1.
4. The composite herbicide according to claim 1, characterized in that the mass ratio of 2-methyl-4-chloro to 2, 3-dihydroxybenzaldehyde is 14:1.
5. The use of a composite herbicide according to any one of claims 1-4 for inhibiting the growth of weeds such as amaranthus retroflexus, eclipta, barnyard grass, crabgrass, gooseberry and nutgrass galingale.
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