CN114573392B - Granular fertilizer for delaying decline of Huanglongbing trees and method - Google Patents

Granular fertilizer for delaying decline of Huanglongbing trees and method Download PDF

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CN114573392B
CN114573392B CN202210241860.9A CN202210241860A CN114573392B CN 114573392 B CN114573392 B CN 114573392B CN 202210241860 A CN202210241860 A CN 202210241860A CN 114573392 B CN114573392 B CN 114573392B
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granular
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sulfate
fertilizer
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CN114573392A (en
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冀建华
候红乾
刘秀梅
蓝贤瑾
吕真真
刘益仁
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Institute of Soil Fertilizer Resources and Environment of Jiangxi Academy of Agricultural Sciences
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B7/00Fertilisers based essentially on alkali or ammonium orthophosphates
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/40Mixtures of one or more fertilisers with additives not having a specially fertilising activity for affecting fertiliser dosage or release rate; for affecting solubility
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/60Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/90Mixtures of one or more fertilisers with additives not having a specially fertilising activity for affecting the nitrification of ammonium compounds or urea in the soil
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00Fertilisers characterised by their form
    • C05G5/10Solid or semi-solid fertilisers, e.g. powders
    • C05G5/12Granules or flakes
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00Fertilisers characterised by their form
    • C05G5/30Layered or coated, e.g. dust-preventing coatings
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/20Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
    • Y02P60/21Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures

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  • Life Sciences & Earth Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Soil Sciences (AREA)
  • Plant Pathology (AREA)
  • Fertilizers (AREA)

Abstract

The invention discloses a granular fertilizer for delaying the decline of a Huanglongbing tree body and a method thereof. The dosage of manganese in the raw materials of the granular fertilizer is increased by about 300 percent, the NPK is reduced by 30 to 50 percent, and the usage amount of the granular fertilizer is 1 to 5kg per plant. The invention discovers that the excessive application of Mn element can inhibit the quantity of Huanglongbing germs in the root system of citrus, and meanwhile, the inhibition effect is better if NPK is reduced by 30-50%, and the special fertilizer designed based on the discovery can well achieve the effect of 'permanent cure' and delay the decline of the Huanglongbing tree body.

Description

Granular fertilizer for delaying decline of Huanglongbing trees and method
Technical Field
The invention belongs to the field of plant disease prevention and control, and particularly relates to a granular fertilizer for delaying the decline of a Huanglongbing tree body and a method.
Background
Citrus is one of the most economically valuable and widely cultivated fruits in the world. China is the world's leading citrus producing area. However, in recent years, orchard soil quality has been drastically reduced due to improper fertilization and improper agricultural management measures. Deterioration of soil quality environment leads to a decrease in plant disease resistance and outbreak of yellow dragon disease (HLB), while resulting in a significant decrease in citrus yield and quality.
Citrus yellow dragon disease (Citrus huanganglingbin), the most harmful Citrus disease in the world, causes enormous losses to the Citrus industry. It is called "cancer" of citrus, and at present, it has endangered the vast major citrus producing areas of our country, such as the major citrus producing areas of florida, california, paul, and the like. Mainly harms major types and types of oranges, tangerines, oranges, lemons, pummelos and the like. Particularly, the disease can be caused all the year round by weak disease resistance of banana oranges, sugar oranges, ponkan oranges, fortunella oranges, tea branch oranges and other varieties, the disease-induced varieties generally have the symptoms of uniform yellowing, mottled yellowing and element-deficiency yellowing, part of the tops of a few branches of an adult tree often yellow in the whole orange garden, and the yellowing branches expand to the whole tree in the second year, so that the tree body is declined. The fruits born by the diseased trees are small, have strange and different shapes, have inclined umbilicus, have smooth and lusterless epidermis and sour taste, have uneven yellow-green color on the epidermis and become orange-red near the pedicles of some varieties, thereby influencing the yield of the citrus, and are called as 'red nose fruit'. The disease has great harm to citrus production, the disease-susceptible varieties lose fruiting capacity and even die within 3-5 years after the disease is found, in recent years, the disease area is continuously enlarged, the bacterial source area is continuously increased, the epidemic situation diffusion speed is continuously improved, the damage loss is very serious, and the disease becomes a large destructive disease for citrus production.
HLB is a bacterial disease caused by the gram-negative bacterium "Candidatus Liberibacter asiaticus" which is parasitic in plant phloem tissue, diaphorina citri being the main vector of transmission. HLB reduces transport of the photosynthetic products, and accumulation of the photosynthetic products affects the flow of other nutrients, resulting in inhibition of citrus growth. The occurrence of citrus HLB is associated with a number of factors. The population of plant-associated microorganisms can directly or indirectly influence the resistance diversity and stability of citrus towards HLB by changing the species of microorganism, which plays a crucial role in various physiological and biochemical processes. In addition, the mineral nutrient balance in the plant can improve the resistance of the plant to the adverse environment. Different citrus varieties have different stress resistance, and some citrus varieties have stronger resistance to HLB. In addition, climate and environmental conditions of citrus plantation can affect the spread of H LB. To date, the technology of digging out diseased trees, chemical pesticides, hybridization and transgenosis is the most important main strategy for controlling HL B, and meanwhile, a series of problems such as environmental pollution, long period and high cost are brought. Therefore, there is an urgent need to find an environmentally friendly and practical method for preventing HLB. Macronutrients and micronutrients are irreplaceable for plant growth, some of which are beneficial for the prevention of plant diseases, and they play a role in activating the plant immune system by altering microbial community structures and metabolic pathways.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides a granular fertilizer for delaying the decline of a Huanglong disease tree body, which specifically adopts the following technical scheme:
a granular fertilizer for delaying the decline of a yellow dragon disease tree body comprises the following raw materials in parts by weight: 4.58-8.9 parts of diamine phosphate, 7.4-15.3 parts of ammonium phosphate, 14-25 parts of potassium nitrate, 7.5-15 parts of ammonium sulfate, 0.9-2.5 parts of urea, 4.5-10 parts of calcium carbonate, 20-40 parts of calcium magnesium nitrate and 2.5-7.5 parts of manganese sulfate.
In the yellow dragon disease stage, citrus shows some symptoms of nutrient deficiency, so that a part of the technicians in the field can supplement nutrients for the direct purpose of supplementing corresponding nutrient elements to relieve the symptoms, which is similar to 'treating symptoms and not treating the root causes' in essence. The inventor deeply knows that the cause of the huanglongbing is the gram negative bacterium 'Candidatus Liberibacter asiaticus' parasitizing in the plant phloem tissue, so that the inventor deeply studies how to inhibit or limit the huanglongbing germs, and finds that when excessive Mn element (more than 3 times of the general dosage) is added, the number of the huanglongbing germs in the root system of the citrus can be well inhibited, the effect of delaying the decay of the huanglongbing tree body is realized, and the 'root cause control' is carried out to a certain extent.
Preferably, the granular fertilizer comprises the following raw materials in parts by weight: 4.58-6.86 parts of diamine phosphate, 7.4-11 parts of ammonium phosphate, 14-21 parts of potassium nitrate, 7.5-11.5 parts of ammonium sulfate, 0.9-1.3 parts of urea, 4.5-7 parts of calcium carbonate, 20-32 parts of calcium magnesium nitrate and 2.5-4.5 parts of manganese sulfate. The inventor also finds that on the basis of adding excessive Mn element, if the amount of NPK is reduced to a certain extent (30-50% less than the ordinary amount) for matching, the reduction of the amount of the yellow dragon germs in the citrus root system is more obvious.
Preferably, the raw materials of the granular fertilizer also comprise 0.35 to 0.7 portion of borax. The inventor also finds that the addition of the element B can well inhibit the amount of yellow shoot in the scion.
Preferably, the raw materials of the granular fertilizer also comprise 0.12 to 0.22 part of copper sulfate, 3.5 to 6.5 parts of ferrous sulfate, 1.3 to 2.5 parts of chelated iron, 0.01 to 0.02 part of ammonium molybdate and 2.8 to 5.5 parts of zinc sulfate. After the addition of trace elements such as iron, zinc, copper, molybdenum and the like, the effect is further improved, the tree vigor of the HLB tree can be remarkably recovered in the next year, and high yield and high quality are realized.
Preferably, the borax is sodium borate decahydrate, the copper sulfate is copper sulfate pentahydrate, the ferrous sulfate is ferrous sulfate heptahydrate, the chelated iron is EDTA chelated iron, the manganese sulfate is manganese sulfate monohydrate, the ammonium molybdate is ammonium heptamolybdate, and the zinc sulfate is zinc sulfate heptahydrate.
The preparation method of the granular fertilizer comprises the following steps: crushing diammonium phosphate, ammonium phosphate, potassium nitrate, ammonium sulfate and urea, uniformly mixing, and granulating under the action of a binder to obtain granules I; crushing calcium carbonate and calcium magnesium nitrate, uniformly mixing, and granulating under the action of a binder to obtain granules II; crushing borax, copper sulfate, chelated iron, ferrous sulfate, manganese sulfate and zinc sulfate, uniformly mixing, spraying ammonium molybdate, and granulating under the action of a binder to obtain granules III; and uniformly mixing the first particle, the second particle and the third particle to obtain the granular fertilizer.
The slow-controlled release fertilizer is a new fertilizer type, and substances are slowly and continuously released through the modes of coating and the like, so that the waste of fertilizer components is avoided, and the use efficiency of the fertilizer is improved. On the basis, the inventor provides a sustained-release granular fertilizer for delaying the decline of the body of the yellow dragon disease based on the discovery of the invention, and the sustained-release granular fertilizer comprises the following raw materials in parts by weight: 5.6-14.12 parts of coated diammonium phosphate, 7.5-11.8 parts of granular ammonium phosphate, 15-30 parts of coated potassium nitrate, 8.5-13.5 parts of coated ammonium sulfate, 1-10 parts of coated urea, 5.05-8.5 parts of granular calcium carbonate, 22.5-37.5 parts of coated calcium magnesium nitrate, 2.8-4 parts of coated manganese sulfate and 0.4-0.7 part of granular water-soluble manganese sulfate.
Preferably, the raw materials of the sustained and controlled release granular fertilizer comprise, by weight: 5.6-14.12 parts of coated diammonium phosphate, 7.5-11.8 parts of granular ammonium phosphate, 15-30 parts of coated potassium nitrate, 8.5-13.5 parts of coated ammonium sulfate, 1-10 parts of coated urea, 5.05-8.5 parts of granular calcium carbonate, 22.5-37.5 parts of coated calcium magnesium nitrate, 2.8-6 parts of coated manganese sulfate and 0.4-1 part of granular water-soluble manganese sulfate.
Preferably, the raw materials of the sustained and controlled release granular fertilizer also comprise 0.41 to 0.68 part of coated borax.
Preferably, the raw materials of the sustained and controlled release granular fertilizer also comprise 0.15 to 0.25 portion of granular copper sulfate, 0.5 to 0.83 portion of granular water-soluble ferrous sulfate, 1.5 to 2.5 portions of granular chelated iron, 3.5 to 5.8 portions of coated ferrous sulfate, 0.01 to 0.02 portion of granular ammonium molybdate, 0.18 to 0.35 portion of granular water-soluble zinc sulfate and 3.1 to 5.1 portions of coated zinc sulfate.
The raw materials are mixed evenly to prepare the sustained and controlled release granular fertilizer. Preferably, the particle size of the granulated and coated fertilizers is 1-2mm. The coated fertilizer is coated by resin. Preferably, the granular copper sulfate, the granular water-soluble ferrous sulfate, the granular chelated iron, the granular water-soluble manganese sulfate, the granular ammonium molybdate and the granular water-soluble zinc sulfate are respectively obtained by crushing copper sulfate pentahydrate, ferrous sulfate heptahydrate, EDTA chelated iron, manganese sulfate monohydrate, ammonium heptamolybdate and zinc sulfate heptahydrate and then granulating under the action of a binder.
The invention also provides a fertilization method for delaying the decline of the Huanglongbing trees based on the discovery and the designed granular fertilizer, which comprises the following steps:
the granular fertilizer is applied to citrus yellow shoot trees for three times, the granular fertilizer is applied as a base fertilizer for the first time, the application standard is 1-2 kg/plant, the granular fertilizer is applied in the tip-extracting flowering phase for the second time, the application standard is 0.5-1.5 kg/plant, the granular fertilizer is applied in the fruit expanding growth phase for the third time, and the application standard is 1.5-2.5 kg/plant; or the controlled-release granular fertilizer is used as a base fertilizer to be applied to the citrus greening disease trees, and the application standard is 1-4kg per tree.
The granular fertilizer and the application method thereof are suitable for oranges, nanfeng tangerines and navel oranges.
The invention has the beneficial effects that:
(1) The invention discovers that the excessive application of Mn element can inhibit the quantity of Huanglongbing germs in the root system of citrus, and meanwhile, the inhibition effect is better if NP K is reduced by 30-50%, and the particle fertilizer designed based on the discovery can well achieve the effect of 'permanent cure' and delay the decline of the Huanglongbing tree body.
(2) The granular fertilizer prepared by the invention can greatly improve the yield of the Gannan navel orange by continuous and multiple application, and obviously improve the quality of the Gannan navel orange.
(3) Compared with the conventional fertilizer special for citrus, the sustained-release granular fertilizer prepared by the invention has the advantages that the sustained-release manganese, the sustained-release boron and the sustained-release zinc are greatly added, and the interactive effect among elements is utilized, so that the content of the pholiopsis citrina of the citrus tree can be effectively reduced by continuous and repeated application, the symptom of the pholiopsis citrina is greatly relieved, the growth of the root system is promoted, and the tree vigor is obviously recovered.
(4) Compared with the existing prevention and control measures (disease tree digging, psylla prevention and tetracycline removal) for citrus yellow shoot, the citrus granular fertilizer prepared by the invention can provide sufficient nutrition for citrus trees, is convenient, efficient, low in cost, fast in synergism, green, environment-friendly and pollution-free.
Detailed Description
The idea and the technical effect of the present invention will be clearly and completely described in the following with reference to the embodiments, so as to fully understand the purpose, the scheme and the effect of the present invention.
Example 1:
a slow and controlled release fertilizer comprises the following raw materials in parts by weight: 6.58 parts of coated diammonium phosphate, 9.22 parts of granular ammonium phosphate, 20 parts of coated potassium nitrate, 11 parts of coated ammonium sulfate, 1.28 parts of coated urea, 5.63 parts of granular calcium carbonate, 28.75 parts of coated calcium magnesium nitrate, 0.62 part of coated manganese sulfate monohydrate and 0.16 part of granular manganese sulfate monohydrate.
Example 2:
a slow and controlled release fertilizer comprises the following raw materials in parts by weight: 14.12 parts of coated diammonium phosphate, 8.77 parts of granular ammonium phosphate, 30 parts of coated potassium nitrate, 12.83 parts of coated ammonium sulfate, 10 parts of coated urea, 5.63 parts of granular calcium carbonate, 28.75 parts of coated calcium magnesium nitrate, 3.1 parts of coated manganese sulfate monohydrate and 0.5 part of granular manganese sulfate monohydrate.
Example 3:
a slow and controlled release fertilizer comprises the following raw materials in parts by weight: 6.58 parts of coated diammonium phosphate, 9.22 parts of granular ammonium phosphate, 20 parts of coated potassium nitrate, 11 parts of coated ammonium sulfate, 1.28 parts of coated urea, 5.63 parts of granular calcium carbonate, 28.75 parts of coated calcium magnesium nitrate, 3.1 parts of coated manganese sulfate monohydrate and 0.5 part of granular manganese sulfate monohydrate.
Example 4:
the slow and controlled release fertilizer comprises the following raw materials in parts by weight: 6.58 parts of coated diammonium phosphate, 9.22 parts of granular ammonium phosphate, 20 parts of coated potassium nitrate, 11 parts of coated ammonium sulfate, 1.28 parts of coated urea, 5.63 parts of granular calcium carbonate, 28.75 parts of coated calcium magnesium nitrate and 0.52 part of coated sodium borate.
Example 5:
a slow and controlled release fertilizer comprises the following raw materials in parts by weight: 6.58 parts of coated diammonium phosphate, 9.22 parts of granular ammonium phosphate-ammonium phosphate, 20 parts of coated potassium nitrate, 11 parts of coated ammonium sulfate, 1.28 parts of coated urea, 5.63 parts of granular calcium carbonate, 28.75 parts of coated calcium magnesium nitrate, 0.52 part of coated sodium borate, 3.1 parts of coated manganese sulfate monohydrate and 0.5 part of granular manganese sulfate monohydrate.
Example 6:
a slow and controlled release fertilizer comprises the following raw materials in parts by weight: 6.58 parts of coated diammonium phosphate, 9.22 parts of particle ammonium phosphate nitrate, 20 parts of coated potassium nitrate, 11 parts of coated ammonium sulfate, 1.28 parts of coated urea, 5.63 parts of particle calcium carbonate, 28.75 parts of coated calcium magnesium nitrate, 0.52 part of coated sodium borate, 3.1 parts of coated manganese sulfate monohydrate, 0.5 part of particle manganese sulfate monohydrate, 0.55 part of particle ferrous sulfate heptahydrate, 1.69 parts of EDTA chelated iron, 4.43 parts of coated ferrous sulfate heptahydrate, 0.16 part of copper sulfate pentahydrate, 0.01 part of ammonium heptamolybdate, 0.23 part of particle zinc sulfate heptahydrate and 3.9 parts of coated zinc sulfate heptahydrate.
Example 7:
set control (ck): a slow and controlled release fertilizer comprises the following raw materials in parts by weight: 14.12 parts of coated diammonium phosphate, 8.77 parts of granular ammonium phosphate nitrate, 30 parts of coated potassium nitrate, 12.83 parts of coated ammonium sulfate, 10 parts of coated urea, 5.63 parts of granular calcium carbonate and 28.75 parts of coated calcium magnesium nitrate (the conventional dosage of NPKCaMg in the controlled release fertilizer for oranges is also the common dosage level in the prior art). The experiments were carried out using examples 1-6 and the control group, as follows.
Test site: ganzhou citrus scientific research institute.
The test varieties are: the test was carried out for 3 years with continuous localization, navel orange variety: a Newhall; the age of the tree: all the trees used in the test were infected navel orange trees for 4 years.
In the test, each treatment is repeated for 3 times, 21 cells are provided, 5 navel orange trees are planted in each cell, the cells are arranged in a random block mode, and the first tree and the last tree of each cell are marked correspondingly.
Fertilization time and method: when base fertilizer is applied every year, the slow-release fertilizer is uniformly scattered on the ground surface along a water dripping line, then the soil is ploughed by 10-20c m to uniformly mix the fertilizer with the soil, and the sampling method comprises the following steps: every year, 3 trees with even growth vigor are selected in each district to measure yield and sample in the mature period of navel oranges, each tree is independently counted to produce, 4 navel orange fruits with even sizes are collected in 4 different directions of each tree to form a sample, quality index and Ct value are measured, 4 root system samples in 4 different directions of each tree are mixed to form a sample, root system form index and Ct value are measured, 12 diseased leaves in 4 different directions of each tree are mixed to form a sample, and Ct value of a vein of each leaf is measured.
The Ct value is measured by real-time fluorescent quantitative PCR, and the leaf, root and fruit samples are measured by real-time fluorescent quantitative PCR after DNA is extracted. The primers used for the real-time fluorescent quantitative PCR are HLBasf and HLBr, and the probe is HLBp. By dd H 2 O and DNA extracted from healthy navel orange material are used as blank control and negative control, respectively, and diseased navel orange DNA is known as positive control. 20 μ L of a fluorescent quantitative PCR amplification system comprising: 10 μ L Bestar qPCR Master Mix, 8 μ L ddH 2 O, 0.4. Mu.L HLBasf (10. Mu. Mol/L), 0.4. Mu.L HLBr (10. Mu. Mol/L), 0.2. Mu.L HLBp (5. Mu. Mol/L) and 1. Mu.L DNA solution. A Real-Time PCR instrument is selected for PCR. The reaction conditions are as follows: 95 ℃ for 10s at 2min,95 ℃;60, 30s,40 cycles.
Root morphology was measured using a root scanner LA2400 and analyzed using a WinRHIO analysis system.
The details of each group are shown in Table 1.
TABLE 1
Figure BDA0003542551630000051
Figure BDA0003542551630000061
The effect on navel orange production results are shown in table 2.
TABLE 2
Treatment of Year one (kg/strain) The second year (kg/strain) Year three (kg/strain)
CK 36.45 35.04 31.97
T1 34.56 32.14 29.55
T2 35.12 33.22 44.19
T3 32.67 39.50 49.15
T4 35.01 36.03 46.85
T5 36.09 45.48 55.95
T6 37.18 47.21 61.06
The effect on navel orange quality is shown in table 3.
TABLE 3
Figure BDA0003542551630000062
The results of the effect on the root system of navel orange are shown in Table 4.
TABLE 4
Figure BDA0003542551630000063
Figure BDA0003542551630000071
The results of the effects on the HLB (Ct) germ content in the root system, leaves and fruits of navel oranges are shown in Table 5.
TABLE 5
Figure BDA0003542551630000072
Note: the detection result is displayed by the cycle number (namely Ct value) when the fluorescence signal in the reaction tube reaches a set threshold, wherein the Ct value is less than or equal to 35, the sample is positive for the Huanglong disease, the Ct value is more than 35, the sample is negative, and the lower the Ct value is, the higher the content of the Huanglong pathogen is.
From the data of tables 2-5, the following conclusions can be found: (1) Excessive application of manganese can inhibit the quantity of yellow dragon disease germs in the root system of the citrus; while the effect of the normal amount of manganese supplemented T1 is not obvious; (2) When the NPK is reduced by 30-50%, the quantity of the manganese which is increased by 300% in matched application can better inhibit the quantity of the yellow dragon disease germs in the root system; (3) The effect of the manganese and boron combined application on inhibiting the content of citrus yellow shoot germs is far better than that of single application; (4) Under the condition that NPK is reduced by 30-50%, the tree vigor of the HLB tree can be remarkably recovered by adding boron, manganese and other trace elements in the second year, and high yield and high quality are realized.
The above description is only a preferred embodiment of the present invention, and the present invention is not limited to the above embodiment, and the present invention shall fall within the protection scope of the present invention as long as the technical effects of the present invention are achieved by the same means. The invention is capable of other modifications and variations in its technical solution and/or its implementation, within the scope of protection of the invention.

Claims (3)

1. A method for delaying the decline of Huanglongbing trees is characterized in that granular fertilizers are applied to citrus Huanglongbing trees, the fertilizers are applied for three times, the first time is used as base fertilizers and is applied according to the application standard of 1-2 kg/plant, the second time is applied in the slight-drawing flowering period, the application standard is 0.5-1.5 kg/plant, the third time is applied in the fruit expanding growth period, and the application standard is 1.5-2.5 kg/plant;
the granular fertilizer comprises the following raw materials in parts by weight: 4.58-6.86 parts of diammonium phosphate, 7.4-11 parts of ammonium phosphate, 14-21 parts of potassium nitrate, 7.5-11.5 parts of ammonium sulfate, 0.9-1.3 parts of urea, 4.5-7 parts of calcium carbonate, 20-32 parts of calcium magnesium nitrate, 2.5-4.5 parts of manganese sulfate and 0.35-0.7 part of borax;
or applying the citrus greening disease tree body by using the sustained and controlled release granular fertilizer as a base fertilizer, wherein the application standard is 1-4kg per plant;
the sustained and controlled release granular fertilizer comprises the following raw materials in parts by weight: 5.6-8 parts of coated diammonium phosphate, 7.5-11.8 parts of granular ammonium phosphate, 15-24 parts of coated potassium nitrate, 8.5-13.5 parts of coated ammonium sulfate, 1-1.65 parts of coated urea, 5.05-8.5 parts of granular calcium carbonate, 22.5-37.5 parts of coated calcium magnesium nitrate, 2.8-4 parts of coated manganese sulfate, 0.4-0.7 part of granular water-soluble manganese sulfate and 0.41-0.68 part of coated borax.
2. The method as claimed in claim 1, wherein the raw materials of the granular fertilizer further comprise, by weight, 0.12 to 0.22 parts of copper sulfate, 3.5 to 6.5 parts of ferrous sulfate, 1.3 to 2.5 parts of chelated iron, 0.01 to 0.02 parts of ammonium molybdate, and 2.8 to 5.5 parts of zinc sulfate.
3. The method as claimed in claim 1, wherein the raw materials of the controlled-release granular fertilizer further comprise, by weight, 0.15 to 0.25 parts of granular copper sulfate, 0.5 to 0.83 parts of granular water-soluble ferrous sulfate, 1.5 to 2.5 parts of granular chelated iron, 3.5 to 5.8 parts of coated ferrous sulfate, 0.01 to 0.02 parts of granular ammonium molybdate, 0.18 to 0.35 parts of granular water-soluble zinc sulfate, and 3.1 to 5.1 parts of coated zinc sulfate.
CN202210241860.9A 2022-03-11 2022-03-11 Granular fertilizer for delaying decline of Huanglongbing trees and method Active CN114573392B (en)

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106748468A (en) * 2016-12-21 2017-05-31 安徽省司尔特肥业股份有限公司 A kind of liquid fertilizer of anti-Citrus Huanglongbing pathogen and preparation method thereof, fertilizing method
CN107522551A (en) * 2017-09-18 2017-12-29 湖南农业大学 A kind of citrus special fertilizer and preparation method thereof and application process
CN109111324A (en) * 2018-09-30 2019-01-01 曲沃县万乡红肥业有限公司 The water-soluble fertilizer of one planting fruit-trees yellow twig prevention and control

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106748468A (en) * 2016-12-21 2017-05-31 安徽省司尔特肥业股份有限公司 A kind of liquid fertilizer of anti-Citrus Huanglongbing pathogen and preparation method thereof, fertilizing method
CN107522551A (en) * 2017-09-18 2017-12-29 湖南农业大学 A kind of citrus special fertilizer and preparation method thereof and application process
CN109111324A (en) * 2018-09-30 2019-01-01 曲沃县万乡红肥业有限公司 The water-soluble fertilizer of one planting fruit-trees yellow twig prevention and control

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