CN114563365A - Detection assembly and method of biological sample optical detection system - Google Patents
Detection assembly and method of biological sample optical detection system Download PDFInfo
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- CN114563365A CN114563365A CN202210197483.3A CN202210197483A CN114563365A CN 114563365 A CN114563365 A CN 114563365A CN 202210197483 A CN202210197483 A CN 202210197483A CN 114563365 A CN114563365 A CN 114563365A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N2021/0106—General arrangement of respective parts
- G01N2021/0112—Apparatus in one mechanical, optical or electronic block
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- Pathology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The invention discloses a detection component of a biological sample optical detection system, which comprises an optical analysis component and a light path component, wherein a light path lens is arranged on the light path component; the lower extreme slidable of second frock piece sets up in the bar spout, and the upper end is the free end, and a side of second frock piece is the line face of setting, is provided with low level net line and high-order net line on the line face of setting, and the net that low level net line and high-order net line formed is the square, and low level net line is close to the lower extreme of second frock piece, and high-order net line is close to the upper end of second frock piece. The invention relates to a state detection method of a biological sample optical detection system, which has novel conception and reasonable design, receives light spots formed on an optical path by arranging a first tooling part and a second tooling part, and realizes the detection of each part on the optical path by comparing the light spots with grids on the second tooling part.
Description
Technical Field
The invention relates to the technical field of biological optical detection, in particular to a detection assembly and a detection method of a biological sample optical detection system.
Background
In current sample analyzers, the optical system plays a very important role.
The concentration of the reaction solution or the absorption of the light energy by the main component contained in the sample by the optical system is converted into an output result, and the output result is converted into absorbance from transmittance.
Therefore, the accuracy of the installation position of the optical system determines the accuracy and reliability of the data processing precision and the performance of the whole sample analyzer.
Generally, an optical system mainly comprises a light source assembly, a light path assembly and an optical analysis system assembly.
The optical path assembly and the optical analysis system assembly are typically mounted on an optical base plate with a colorimetric container containing the reaction solution therebetween.
Light emitted from the light source component can hit a colorimetric container containing the reaction solution through the light path component.
When light with a certain light spot size passes through the reaction solution, the optical monochromator assembly receives light energy transmitted by the light spot size, and then the concentration of the reaction solution or the content of a certain sample component is analyzed by a spectrophotometry method to calculate the absorbance for the absorption (transmission) of the light energy.
The accuracy of the position of the light spot of the light path component on the colorimetric container is very important for the performance of the whole machine, namely the size, the front and back, the left and right, and the up and down positions of the light spot of the light path irradiating the reaction solution in the colorimetric container are directly influenced.
At present, no efficient and accurate debugging method, inspection method or detection tool is provided for common analyzers in the market.
Therefore, in the debugging process of the optical system, the defects that the uncertainty factors caused by artificial debugging errors, installation part machining errors and optical element process errors cannot be accurately measured and optimized and the like exist, and the most important point is that the analysis precision, the performance stability and the reliability of the whole machine are greatly influenced.
Disclosure of Invention
In view of the defects that in the debugging process of an optical system, uncertainty factors caused by artificial debugging errors, installation part machining errors and optical element process errors cannot be accurately measured and optimized, and the like, the most important problem is that the analysis precision, the performance stability and the reliability of the whole optical detection system are greatly influenced.
The invention is realized by the following technical scheme:
a detection component of a biological sample optical detection system comprises an optical analysis component and a light path component, wherein a light path lens is arranged on the light path component, and a first tool piece and a second tool piece are arranged between the optical analysis component and the light path component;
a bar-shaped sliding groove is formed in the first tooling part, and a graduated scale is arranged along the edge of the bar-shaped sliding groove;
the lower extreme slidable of second frock piece set up in the bar spout, the upper end is the free end, a side of second frock piece sets up the face for the line, the line sets up the face towards the light path subassembly, the line sets up and is provided with low level net line and high level net line on the face, just the net that low level net line and high level net line formed is the square, low level net line is close to the lower extreme of second frock piece, high level net line is close to the upper end of second frock piece.
In some embodiments, the optical assembly further comprises a second tool, wherein the second tool is configured to be mounted to the optical chassis.
In some embodiments, the first tooling component is disposed on the optical backplane by a locating pin.
In some embodiments, the apparatus further comprises a heating block, and the first tool, the second tool, the optical analysis component, and the optical path component are disposed on the heating block.
In some embodiments, the first tooling component is disposed on the heating seat by a locating pin.
In some embodiments, the first tooling member and the second tooling member are each rectangular block-shaped.
In some embodiments, the low-order gridlines and the high-order gridlines are both grid-like.
The second objective of the present invention is to provide a method for detecting the state of a biological sample optical detection system, comprising the following steps:
taking an optical bottom plate, arranging an optical analysis component and a light path component on the optical bottom plate, and setting a light source arrangement position on the optical bottom plate, wherein the light source arrangement position, the light path component and the optical analysis component form a light path for detection;
setting a sample detection position on the optical path and between the optical analysis component and the optical path component;
installing a first tool piece at the sample detection position, and installing a second tool piece on the first tool piece;
setting a light source assembly at the light source arrangement position, wherein light rays emitted by the light source assembly penetrate through the light path assembly along a light path and irradiate the high-position grid pattern of the second workpiece to form a first light spot;
and sliding the second tooling part on the first tooling part, enabling the first light spot to move at the high-position grid texture, and judging the appropriate condition of the light path component according to the alignment of the first light spot and the corresponding grid in the moving process.
Further, in some embodiments, the step of taking an optical substrate and disposing an optical analysis component and an optical path component on the optical substrate, and setting a light source mounting position on the optical substrate, the light source mounting position forming an optical path for detection with the optical path component and the optical analysis component comprises:
and an optical lens is arranged on the light path component.
Further, in some embodiments, the method further comprises the steps of:
a reaction disc heating seat is arranged on the optical bottom plate;
a mounting position corresponding to the sample detection position is arranged on the reaction disc heating seat, the first tooling part is mounted in the mounting position, and the second tooling part is mounted on the first tooling part;
opening the light source assembly, wherein light rays emitted by the light source assembly penetrate through the light path assembly along a light path and irradiate the low-position grid pattern of the second workpiece to form a second light spot;
and judging the quality of the heating seat of the reaction disk by comparing the second light spot with the first light spot.
Compared with the prior art, the invention has the following advantages and beneficial effects.
The invention has novel conception and reasonable design, receives light spots formed on an optical path by arranging the first tooling part and the second tooling part, and realizes the detection of each part on the optical path by comparing the light spots with grids on the second tooling part, wherein the content which can be judged by the detection specifically comprises the following contents:
firstly, checking whether the installation size of an optical lens assembled on an optical path seat of the optical path component is qualified or not, and particularly whether the machining of a 45-degree inclined plane of the installation seat of the optical path component is qualified or not;
checking whether the optical lens of the optical path component is assembled in place;
checking whether optical lenses (lenses and reflectors) of the optical path component are qualified;
checking whether the front stage optical path in the optical system is collimated;
checking the focusing position of the imaging light spot of the preceding stage light path in the optical system, which is favorable for researching the minimum reaction volume of the cuvette solution of the analyzer;
sixthly, checking whether the heating seat of the reaction disc is processed to be qualified.
Drawings
In order to more clearly illustrate the technical solutions of the exemplary embodiments of the present invention, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and that for those skilled in the art, other related drawings can be obtained from these drawings without inventive effort. In the drawings:
FIG. 1 is a schematic structural diagram of an embodiment of the present invention;
FIG. 2 is an enlarged schematic view of section E of FIG. 1;
fig. 3 is a schematic view of an assembly structure of the first tooling member and the second tooling member in the present invention.
Reference numbers and corresponding part names in the drawings:
the device comprises a first tool part-100, a strip-shaped chute-110 and a graduated scale-111;
a second workpiece-200, a texture setting surface-210, a low-position grid texture-211 and a high-position grid texture-212;
optical analysis assembly-300;
an optical path component-400;
a bottom plate-500;
sample detection position-A, light source installation position-B, light path-L.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail below with reference to examples and accompanying drawings, and the exemplary embodiments and descriptions thereof are only used for explaining the present invention and are not meant to limit the present invention.
Referring to fig. 1-3, a testing assembly of an optical testing system for biological samples includes a first tool 100, a second tool 200, an optical analysis assembly 300, and an optical path assembly 400.
The optical analysis module 300 is an analysis device assembly for receiving light, and the light enters the optical analysis module 300 to be analyzed.
The light path component 400 is a light transmissive or reflective component disposed on the light path. The optical path lens is provided with a lens and a reflector.
The first tool 100 and the second tool 200 are used as a whole when embodying the function.
The first tool 100 and the second tool 200 are disposed between the optical analysis assembly 300 and the optical path assembly 400.
The first tool 100 is used for positioning, i.e. directly positioned and arranged at a setting position for setting a sample to be tested.
For sliding the second tool 200, a strip-shaped chute 110 is provided on the first tool 100. In order to quantitatively determine the moving position of the second tooling member 200 on the first tooling member 100, a scale 111 is disposed along the edge of the strip-shaped chute 110.
In some specific embodiments, the first tool 100 and the second tool 200 are each rectangular block-shaped.
The lower end of the second tool 200 is slidably disposed in the bar-shaped sliding groove 110, and the upper end is a free end.
As shown in fig. 3, one side surface of the second tool part 200 is a texture setting surface 210, and the texture setting surface 210 faces the optical path component 400.
The texture arrangement surface 210 is provided with low-order grid textures 211 and high-order grid textures 212, and grids formed by the low-order grid textures 211 and the high-order grid textures 212 are squares.
The lower grid pattern 211 is close to the lower end of the second tool 200, and the upper grid pattern 212 is close to the upper end of the second tool 200.
In some embodiments, the lower gridlines 211 and the upper gridlines 212 are all matted.
As shown in fig. 3, each of the lower grid patterns 211 and the upper grid patterns 212 includes a plurality of rectangular lattices, and any adjacent four lattices may form a specific shape of a field.
In order to stably arrange the components, in some embodiments, the optical base plate 500 is further included, and the first tool 100, the second tool 200, the optical analysis assembly 300, and the optical path assembly 400 are arranged on the optical base plate 500.
Further, in some embodiments, the first tooling 100 is disposed on the optical backplane 500 by positioning pins.
In order to meet the requirement of optical detection, the first tooling member 100 and the second tooling member 200 are both fine tooling members. The first tool 100 and the second tool 200 are assembled on the optical backplane 500 by providing positioning pins, so that the first tool 100 and the second tool 200 can be conveniently assembled and disassembled.
In order to perform the detection on the reaction disk heating seat, in some embodiments, the reaction disk heating seat is further included, and the first tool 100, the second tool 200, the optical analysis assembly 300, and the optical path assembly 400 are disposed on the reaction disk heating seat.
Further, to facilitate mounting and dismounting, in some embodiments, the first tooling member 100 is disposed on the reaction tray heating seat by a positioning pin.
A state detection method of a biological sample optical detection system comprises the following steps:
s100, taking the optical substrate 500, and disposing the optical analysis component 300 and the optical path component 400 on the optical substrate 500, and setting a light source mounting position B on the optical substrate 500, where the light source mounting position B, the optical path component 400 and the optical analysis component form an optical path L for detection.
The optical detection system for detecting biological samples comprises an optical analysis component 300 for analyzing received light and an optical path component 400 for transmitting light. In order to realize the optical path for detection, a light source mounting position B is set on the optical substrate 500 for mounting the light source. The light source generates light, which passes through the light path assembly 400 and irradiates into the optical analysis assembly 300, so as to analyze the light.
Forming the optical path L is necessary to realize the post-detection of the sample.
S200, setting a sample detection position a on the optical path and between the optical analysis component 300 and the optical path component 400.
Since sample detection requires that a sample be placed in the detection optical path in order to perform detection, a sample detection position a is provided between the optical analysis assembly 300 and the optical path assembly 400 for mounting a sample detection device, as shown in fig. 2.
S300, a first tool 100 is attached to the sample detection position a, and a second tool 200 is attached to the first tool 100.
The two tooling parts are used for detecting light rays generated by the light path, and the light rays are used for irradiating the sample arrangement position. The first tool 100 is thus arranged at the sample detection position a for detecting the light path.
S400, setting a light source assembly at the light source positioning position B, wherein light emitted by the light source assembly penetrates through the light path assembly 400 along a light path and irradiates the high-position grid pattern 212 of the second workpiece 200 to form a first light spot.
The light source assembly emits light, the light passes through the light path assembly 400 through the light path, and irradiates the high-order grid pattern 212 of the second workpiece 200 to form a first light spot, so as to complete the projection of the light spot.
S500, sliding the second tooling member 200 on the first tooling member 100, moving the first light spot at the high-position grid pattern 212, and determining the suitable condition of the light path component 400 according to the alignment between the first light spot and the corresponding grid during the moving process.
The light spot formed by the light is received by adjusting the second tool part 200, and then the specific comparison and judgment are realized through the movement of the first light spot.
Since the first light spot is in a grid shape in actual implementation, a contrast can be achieved by shifting and the high-order grid pattern 212.
Further, in some embodiments, the step S100 includes:
an optical lens is disposed on the optical path assembly 400.
In a specific implementation, the optical lens is a lens or a mirror.
Further, in some embodiments, the method further comprises the steps of:
s001, arranging a reaction disc heating seat on the optical bottom plate 500.
The reaction plate is prepared for detection by arranging a reaction plate heating seat.
And S002, arranging a position corresponding to the sample detection position A on the reaction disc heating seat, installing the first tooling part 100 in the arranging position, and installing the second tooling part 200 on the first tooling part 100.
In order to detect the reaction disk heating seat, the first tool 100 and the second tool 200 are disposed at corresponding positions. The corresponding position is the location of the sample detection position a.
And S003, opening the light source assembly, wherein light rays emitted by the light source assembly penetrate through the light path assembly 400 along a light path and irradiate the low-position grid pattern 211 of the second tool part 200 to form a second light spot.
When the light source assembly is turned on, light is generated. The light passes through the light path assembly 400 and irradiates the lower grid pattern 211 to form a second light spot.
And S004, judging the quality of the reaction disk heating seat by comparing the second light spot with the first light spot.
The above-mentioned embodiments are intended to illustrate the objects, technical solutions and advantages of the present invention in further detail, and it should be understood that the above-mentioned embodiments are merely exemplary embodiments of the present invention, and are not intended to limit the scope of the present invention, and any modifications, equivalent substitutions, improvements and the like made within the spirit and principle of the present invention should be included in the scope of the present invention.
Claims (10)
1. A detection component of an optical detection system for biological samples, comprising an optical analysis component (300) and an optical path component (400), wherein an optical path lens is arranged on the optical path component (400), and further comprising a first tool (100) and a second tool (200) which are arranged between the optical analysis component (300) and the optical path component (400);
a bar-shaped sliding groove (110) is formed in the first tool part (100), and a graduated scale (111) is arranged along the edge of the bar-shaped sliding groove (110);
the lower extreme slidable of second frock piece (200) set up in bar spout (110), the upper end is the free end, a side of second frock piece (200) sets up face (210) for the line, line set up face (210) towards light path subassembly (400), line set up and be provided with low position grid line (211) and high position grid line (212) on face (210), just the grid that low position grid line (211) and high position grid line formed is the square, low position grid line is close to the lower extreme of second frock piece (200), high position grid line (212) are close to the upper end of second frock piece (200).
2. The detection assembly of the optical detection system for biological samples according to claim 1, further comprising an optical base plate (500), wherein the first tool (100), the second tool (200), the optical analysis assembly (300) and the optical path assembly (400) are disposed on the optical base plate (500).
3. The detection assembly of the optical detection system for biological samples according to claim 2, wherein the first tool member (100) is disposed on the optical base plate (500) by a positioning pin.
4. The optical detection assembly of claim 1, further comprising a reaction tray heating base, wherein the first tool (100), the second tool (200), the optical analysis assembly (300) and the optical path assembly (400) are disposed on the reaction tray heating base.
5. The optical detection assembly of biological sample according to claim 4, wherein the first tool member (100) is disposed on the reaction tray heating seat by a positioning pin.
6. The optical detection assembly of biological samples according to claim 1, wherein the first and second tools (100, 200) are rectangular blocks.
7. The optical detection assembly of biological sample according to claim 1, wherein the lower grid (211) and the upper grid (212) are grid-shaped.
8. A state detection method of a biological sample optical detection system is characterized by comprising the following steps:
taking an optical bottom plate (500), arranging an optical analysis component (300) and an optical path component (400) on the optical bottom plate (500), and setting a light source mounting position (B) on the optical bottom plate (500), wherein the light source mounting position (B), the optical path component (400) and the optical analysis component form an optical path (L) for detection;
-setting a sample detection position (a) in said optical path and between said optical analysis assembly (300) and said optical path assembly (400);
-providing a first tool (100) at the sample detection position (a), providing a second tool (200) on the first tool (100);
setting a light source assembly at the light source arrangement position (B), wherein light rays emitted by the light source assembly penetrate through the light path assembly (400) along a light path and irradiate the high-position grid pattern (212) of the second tool part (200) to form a first light spot;
and sliding the second tool part (200) on the first tool part (100), wherein the first light spot moves at the high-position grid texture (212), and the suitable condition of the light path component (400) is judged according to the alignment of the first light spot and the corresponding grid in the moving process.
9. The method as claimed in claim 8, wherein the step of taking the optical base plate (500), disposing the optical analysis module (300) and the optical path module (400) on the optical base plate (500), and setting the light source mounting position (B) on the optical base plate (500), the light source mounting position (B) forming the light path (L) for detection with the optical path module (400) and the optical analysis module comprises:
an optical lens is arranged on the light path component (400).
10. The method for detecting the state of an optical inspection system for biological samples according to claim 1, further comprising the steps of:
a reaction disc heating seat is arranged on the optical bottom plate (500);
a setting position corresponding to the sample detection position (A) is arranged on the reaction disc heating seat, the first tool piece (100) is arranged in the setting position, and the second tool piece (200) is arranged on the first tool piece (100);
opening the light source assembly, wherein light rays emitted by the light source assembly penetrate through the light path assembly (400) along a light path and irradiate the low-position grid pattern (211) of the second tool (200) to form a second light spot;
and judging the quality of the heating seat of the reaction disk by comparing the second light spot with the first light spot.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210197483.3A CN114563365A (en) | 2022-03-01 | 2022-03-01 | Detection assembly and method of biological sample optical detection system |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210197483.3A CN114563365A (en) | 2022-03-01 | 2022-03-01 | Detection assembly and method of biological sample optical detection system |
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| Publication Number | Publication Date |
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| CN114563365A true CN114563365A (en) | 2022-05-31 |
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| CN202210197483.3A Pending CN114563365A (en) | 2022-03-01 | 2022-03-01 | Detection assembly and method of biological sample optical detection system |
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| CN (1) | CN114563365A (en) |
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- 2022-03-01 CN CN202210197483.3A patent/CN114563365A/en active Pending
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