CN114557936A

CN114557936A - Wolfberry lipstick and preparation method thereof

Info

Publication number: CN114557936A
Application number: CN202210303095.9A
Authority: CN
Inventors: 丁奎虎; 杨佳潇; 张卓阳; 张琪琪; 田金成; 游南林; 杨晨; 丁晓峰; 马立虎; 曹相玫
Original assignee: Ningxia Medical University
Current assignee: Ningxia Medical University
Priority date: 2022-03-24
Filing date: 2022-03-24
Publication date: 2022-05-31

Abstract

The invention discloses a medlar lipstick and a preparation method thereof, wherein the medlar lipstick comprises 5-15 parts of rose essence, 5-20 parts of beeswax, 20-30 parts of medlar polysaccharide extract, 10-20 parts of medlar red pigment extract, 7-10 parts of olive oil, 7-10 parts of glycerol, 1-5 parts of preservative, 3-6 parts of pearl powder, 2-6 parts of diisostearyl malate and 3-5 parts of edible pigment.

Description

Wolfberry lipstick and preparation method thereof

Technical Field

The invention relates to the technical field of daily chemical products, and particularly relates to a wolfberry lipstick and a preparation method thereof.

Background

The lipstick is a necessary cosmetic in daily life, and has the effects of moisturizing, preventing dryness and crack of lips and the like. The lipsticks which are frequently used in daily life mostly contain substances which are harmful to human bodies, such as hormones, pigments and the like, and people can easily eat the lipsticks by mistake when applying the lipsticks to the lips, so that the problems of the human bodies can be caused for a long time.

Disclosure of Invention

In view of the above, there is a need for a healthy wolfberry lipstick.

A preparation method of the wolfberry lipstick is also provided.

A wolfberry lipstick comprises 5-15 parts of rose essence, 5-20 parts of beeswax, 20-30 parts of wolfberry polysaccharide extract, 10-20 parts of wolfberry red pigment extract, 7-10 parts of olive oil, 7-10 parts of glycerol, 1-5 parts of preservative, 3-6 parts of pearl powder, 2-6 parts of diisostearyl malate and 3-5 parts of edible pigment.

The preparation method of the wolfberry lipstick utilizes the wolfberry lipstick raw material as described above for preparation, and comprises the following specific steps:

first dissolved material: dissolving 5-20 parts of beeswax at a first preset temperature to obtain a first dissolved material, and then filtering the first dissolved material to obtain a first filtrate;

second dissolved material: stirring and dissolving 7-10 parts of olive oil, 7-10 parts of glycerol, 5-15 parts of rose essence and 3-5 parts of edible pigment at a second preset temperature to obtain a second dissolved material, and then filtering the second dissolved material to obtain a second filtrate;

mixing: mixing and stirring the first filtrate, the second filtrate, 1-5 parts of preservative, 3-6 parts of pearl essence and 2-6 parts of diisostearyl malate to obtain a first mixture, adding 20-30 parts of lycium barbarum polysaccharide extract into the first mixture, and stirring the mixture to be completely melted under a preset temperature gradient; obtaining a second mixture;

filling and forming: the second mixture is centrifuged, filtered, and the filtrate is collected and poured into a mold at a fourth predetermined temperature.

Preferably, in the first step of dissolving the material, the first predetermined temperature is 90 ℃ to 130 ℃.

Preferably, in the second step of dissolving the material, the second predetermined temperature is 70 ℃ to 100 ℃.

Preferably, in the mixing step, the predetermined temperature gradient is that the first filtrate, the second filtrate, 1-5 parts of preservative, 3-6 parts of pearl powder and 2-6 parts of diisostearyl malate are stirred at 80-95 ℃ for a first predetermined time to obtain a premix, the temperature is reduced to 35-40 ℃, 20-30 parts of lycium barbarum polysaccharide extract and 10-20 parts of lycium barbarum pigment extract are added into the premix and stirred for a second predetermined time to obtain a first mixture.

Preferably, in the mixing step, the first predetermined time is 0.8 to 1.0 hour, and the second predetermined time is 0.5 to 1.0 hour.

Preferably, in the mixing step, the extraction method of the lycium barbarum polysaccharide extract comprises the following steps:

s1: crushing the medlar to obtain medlar powder, and adding pectinase and papain into the medlar powder to ensure that the medlar powder is degreased and kept nearly colorless to obtain a first product;

s2: filtering the first product to obtain a first product filtrate, adding distilled water into the first product filtrate, and performing ultrasonic leaching for 4-6 hours at the temperature of 80-100 ℃ to obtain a second product;

s3: centrifuging the second product at normal temperature, taking supernatant, filtering, and collecting the filtrate of the second product;

s4: performing rotary evaporation on the second product filtrate collected in the step S3 to obtain a third product, washing the third product with 95% ethanol, absolute ethyl alcohol and acetone in sequence, and then performing vacuum drying to obtain a concentrated solution;

s5: adding ethanol into the concentrated solution, standing, precipitating with ethanol, removing ethanol, and drying to obtain fructus Lycii polysaccharide.

Preferably, in the step S1: the weight ratio of the medlar powder to the pectinase and the papain is 1: 10-40: 10-40.

Preferably, in the mixing step, the extraction method of the lycium barbarum red pigment extract comprises the following steps:

pretreatment: soaking fructus Lycii in 0.lml/L sodium hydroxide solution for 22-26 hr, filtering, washing with clear water until the water solution is neutral, drying at 35-45 deg.C for 22-26 hr, and placing into a sealed container;

and (3) extraction of the medlar red pigment: soxhlet extraction of wolfberry fruit at 35-45 deg.c to obtain red pigment, rotary steaming the extracted liquid until the extracted liquid is concentrated to viscous state, and drying in vacuum drier to obtain red pigment product.

Preferably, in the filling and forming step, the fourth predetermined temperature is 70-80 ℃.

By adopting the technical scheme, the invention has the beneficial effects that:

(1) the raw materials added in the preparation of the wolfberry lipstick are green raw materials, so that people can moisten lips and keep faint scent after using the wolfberry lipstick, and people do not worry about the influence on the health of people after eating the wolfberry lipstick by mistake.

(2) The wolfberry fruit polysaccharide extract and the wolfberry fruit red pigment extract are added into the wolfberry fruit lipstick, so that lips of people can have color after the lipstick is used, the wolfberry fruit polysaccharide extract has the effects of reducing blood sugar, protecting blood vessels, resisting oxidation and ageing, the wolfberry fruit red pigment extract contains carotene, lutein and other substances, the human body immunity function can be improved, tumors can be prevented and inhibited, atherosclerosis can be prevented, and the wolfberry fruit polysaccharide extract is beneficial to human bodies after people eat the lipstick by mistake.

(3) According to the invention, the lycium barbarum polysaccharide extract and the lycium barbarum red pigment extract are added into the lycium barbarum lipstick, so that the apoptosis of lips can be reduced, the collagen fibers of the lips are protected from being damaged, and good moisturizing and moistening effects are achieved.

Drawings

FIG. 1 histological observation of rat skin (HE staining).

FIG. 2 staining of rat skin fibers.

Detailed Description

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following will further describe the embodiments.

The embodiment of the invention provides a medlar lipstick which comprises 5-15 parts of rose essence, 5-20 parts of beeswax, 20-30 parts of medlar polysaccharide extract, 10-20 parts of medlar red pigment extract, 7-10 parts of olive oil, 7-10 parts of glycerol, 1-5 parts of preservative, 3-6 parts of pearl powder, 2-6 parts of diisostearyl malate and 3-5 parts of edible pigment.

preparing materials: checking whether the raw materials are qualified;

first dissolved material: adding 5-20 parts of beeswax into a heating pot, dissolving the beeswax at a first preset temperature to obtain a first dissolved material, and then filtering the first dissolved material to obtain a first filtrate;

second dissolved material: adding 7-10 parts of olive oil, 7-10 parts of glycerol, 5-15 parts of rose essence and 3-5 parts of edible pigment into a vacuum double-material pot, stirring and dissolving at a second preset temperature to obtain a second dissolved material, and then filtering the second dissolved material to obtain a second filtrate;

filling and forming: centrifuging and filtering the second mixture, collecting the filtrate, and filling the filtrate into a mold at a fourth predetermined temperature.

In particular, the first dissolving material step is dissolved separately from the second dissolving material step, because: the main chemical components of beeswax are classified into 4 categories, namely esters, free acids, free alcohols and hydrocarbons, and in addition, the beeswax also contains trace volatile oil and pigments, and the rose essence contains abundant vitamin C, saccharides, non-volatile acids, flavonoid compounds and various pigments, and also contains various amino acids and trace elements which are necessary for human bodies. The mixture is separately dissolved and filtered to avoid the reaction of lipid in the beeswax and the non-volatile acid in the rose essence, thereby effectively ensuring the purity and the beneficial components of the beeswax and the rose essence.

Specifically, the preservative may be one of hydroxybenzoate, propyl p-hydroxybenzoate and sorbic acid.

Further, in the first material dissolving step, the first preset temperature is 90-130 ℃.

Further, in the second material dissolving step, the second preset temperature is 70-100 ℃.

Further, in the mixing step, the predetermined temperature gradient is that the first filtrate, the second filtrate, 1-5 parts of preservative, 3-6 parts of pearl powder and 2-6 parts of diisostearyl malate are stirred and mixed at 80-95 ℃, stirred for a first predetermined time to obtain a first mixture, then the temperature is reduced to 35-40 ℃, 20-30 parts of lycium barbarum polysaccharide extract and 10-20 parts of lycium barbarum red pigment extract are added into the premix and stirred for a second predetermined time to obtain a second mixture, and the influence of high temperature on the stability of the lycium barbarum extract is avoided.

Further, in the mixing step, the first predetermined time is 0.8-1.0h, and the second predetermined time is 0.5-1.0 h.

Further, in the mixing step, the extraction method of the lycium barbarum polysaccharide extract comprises the following steps:

s2: filtering the first product to obtain a first product filtrate, adding distilled water into the first product filtrate, and performing ultrasonic leaching at 80-100 ℃ for 4-6h to obtain a second product;

s4: performing rotary evaporation on the second product filtrate collected in the step S3 to obtain a third product, washing the third product with 95% ethanol, absolute ethyl alcohol and acetone in sequence to dissolve the third product and keep the third product in a solution state, and then performing vacuum drying to obtain a concentrated solution;

Specifically, the lycium barbarum red pigment extract can be prepared by using Chinese invention patent with application number CN201210543222.9, and can also be obtained from the market.

Further, in the step S1: the weight ratio of the medlar powder to the pectinase and the papain is 1: 10-40: 10-40.

Further, in the mixing step, the extraction method of the lycium barbarum red pigment extract comprises the following steps:

pretreatment: soaking the medlar in 0.lml/L sodium hydroxide solution for 22 to 26 hours, then filtering, washing the medlar with clear water until the water solution is neutral, drying for 22 to 26 hours at the temperature of between 35 and 45 ℃, and putting the medlar into a closed container for later use;

Specifically, the lycium barbarum red pigment extract can be prepared by using a Chinese invention patent with the application number of CN 201410748202.4; or may be purchased from the market.

Further, in the filling and forming step, the fourth preset temperature is 70-80 ℃.

The invention is further illustrated by the following experiments.

Extracting the lycium barbarum polysaccharide by the extraction method of the lycium barbarum polysaccharide extract, crushing lycium barbarum to obtain 100g of lycium barbarum powder, adding pectinase and papain to the lycium barbarum powder, and mixing the lycium barbarum polysaccharide extract with the lycium barbarum powder in a proportion of 1: 20: 20 to obtain a first product; filtering the first product, collecting filtrate, adding 200g of distilled water into the filtrate, and performing ultrasonic leaching at 90 ℃ for 6 hours to obtain a second product; centrifuging the second product at normal temperature, taking supernatant, filtering, and collecting filtrate; carrying out rotary evaporation on the filtrate to obtain a third product, washing the third product with 95% ethanol, absolute ethanol and acetone in sequence, and then drying in vacuum to obtain a concentrated solution; adding ethanol into the concentrated solution, standing for precipitating with ethanol, pouring out ethanol, and drying to obtain fructus Lycii polysaccharide with purity of 98%.

1. Materials:

female SD rats (SPF grade) of experimental animals 21 to 25 d were provided by the experimental animals center of the university of ningxia medical science [ animal certification numbers: SCXK (Nine) 2019-. The food is fed in cages with regular illumination and free diet, and has no adverse influence factors.

Glycerol (beijing bootoda technologies ltd), Masson staining kit (shanghai solibao biotechnology ltd). Antibody Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb (cell signaling technology Co.)

2. Instrument for measuring the position of a moving object

An ultraviolet lamp high-pressure pump lamp (Beijing Tianmai constant glow light source electric appliance Co., Ltd.), LEICAASP200 computer tissue dehydrator; LEICAEG116 embedding machine; leicirm 2145 paraffin embedding microtome; and (3) spreading and baking the CS-IV slices.

3. Animal grouping and modeling

40 rats were randomly divided into 4 groups: control (blank group), long wave Ultraviolet (UVA) group, UVA + lycium barbarum polysaccharide group, UVA + glycerol group, 10 in each group. The back fur is removed by adopting a fur pushing device special for animals, and the exposed area is 2cm multiplied by 3 cm. Adding fructus Lycii polysaccharide powder into glycerol, and stirring with glass cup to obtain fructus Lycii polysaccharide glycerol suspension (fructus Lycii polysaccharide: 0.3 g/ml). The Control group and UVA group were not treated, 0.6 mL of glycerin was applied to each back of UVA + glycerin group, 0.6 mL of Lycium barbarum polysaccharide glycerin suspension was applied to each back of UVA + Lycium barbarum polysaccharide group, 1 time before each irradiation of ultraviolet light, and the remaining 3 groups except Control group were placed at a distance of 30cm from ultraviolet lamp (300W), that is, rats received energy of 0.0265J cm^-2·s^-1The rat model of skin lesions was made by irradiation for 0.5h/d for one week continuously. Skin tissues of the dorsal epilation area were taken, fixed with paraformaldehyde, embedded in paraffin, and sectioned for the following experiments.

HE staining

Hematoxylin is a basic dye that stains basophilic structures bluish violet. Eosin is an acid dye that dyes eosinophilic structures red. Dewaxing and rehydrating before dyeing, putting the slices into hematoxylin water solution for dip dyeing for 10 minutes during dyeing, washing off floating color, separating color of color separation liquid for several seconds, and then fully washing with running water. Then the obtained product is placed into eosin staining solution for staining for 1 minute and is fully washed by water. The sections were finally incubated 2 times in 95% ethanol for 10 seconds each. Incubations were repeated 2 times in 100% ethanol for 10 seconds each. The incubation in xylene was repeated 2 times for 10 seconds each. Coverslipping and mounting medium were used.

Masson staining

Paraffin sections were dewaxed and rehydrated and stained according to Masson staining instructions. Firstly, staining for 5min-10min by using Weigart iron hematoxylin staining solution. Then the obtained mixture is put into an acidic ethanol differentiation solution for short-term differentiation and is fully washed. Then, the mixture is dyed by Masson blue dyeing solution for 3-5min and then is fully washed by water. And dyeing in the ponceau fuchsin dyeing solution for 5-10min, sequentially soaking and washing for 2min by using weak acid working solution, phosphomolybdic acid solution and weak acid working solution, putting into aniline blue dyeing solution for dyeing for 1-2min, fully washing by using the weak acid working solution, and finally incubating and slicing in 95% ethanol for 2 times, wherein each time lasts for 10 seconds. Incubations were repeated 2 times in 100% ethanol for 10 seconds each. The incubation in xylene was repeated 2 times for 10 seconds each. Coverslipping and mounting reagent mounting were used. The staining was observed under a microscope, and collagen fibers were stained blue, cell nuclei were stained black blue, and muscle fibers were stained red.

6. Immunohistochemistry and outcome determination

Dewaxing and rehydrating the slices, repairing antigens, dewaxing and rehydrating firstly, then repairing the antigens, blocking endogenous peroxidase by using 3% hydrogen peroxide for 10 minutes at room temperature, washing by PBS, then dropping 5% goat serum into a 37 ℃ incubator for 30 minutes, then adding a drop of clean Caspase-3 into each slide, and standing overnight at 4 ℃. And (3) adding a polymer reinforcing agent after the PBS is washed the second day, putting the slices into a 37 ℃ incubator for 30min, adding a drop of mouse anti-rabbit serum into each slice after the PBS is washed, putting the slices into the 37 ℃ incubator for 1h, adding DAB color developing solution, and finally counterstaining with hematoxylin dye solution. Observing, dehydrating and sealing.

All results are obtained by two experimenters observing under an optical microscope set under the same condition and obtaining consistent opinions. The sections were placed under a high power lens and 10 fields were randomly selected. The criteria are as follows: (1) and judging the number of positive cells: scoring the percentage of positive cells in the total number of cells, scoring 0 as 0, 1-25% as 1, 26-50% as 2, 51-75% as 3, and >75% as 4; (2) and determining the degree of cell staining (compared with background staining): the color was scored as 0 point for no color, 1 point for light yellow, 2 points for brown and 3 points for tan. The product of the two scores is the immunoreactivity intensity distribution index (IRIDI). A larger value of IRIDI indicates a larger expression level of cleared Caspase-3 in the tissue.

Statistical analysis of experimental data was performed using SPSS 25.0, one-way anova was used to compare differences in skin thickness for different groups of rats, Kruskal-Wallis H test using multiple independent sample comparisons to compare differences in clear Caspase-3 protein IRIDI for different groups, and pairwise tests for each group were performed. Take a =0.01 as the test standard.

7. As a result, the

(1) Morphological observation of skin tissue

Successfully establishes a rat skin photoaging model. The HE dyeing result shows that the skin structure of the Control group (figure 1 a) is complete, the horny layer of the skin has no thickening phenomenon, the skin thickening is obviously different from the thickening of the epidermis of the UVA group (P is less than 0.01), the distribution is uniform, inflammatory cells are not infiltrated, the distribution of fur sebaceous glands is uniform, the content of light pink fibers is rich, the thickness is uniform, and the shape is smooth; in the UVA group (FIG. 1 b) there is hyperkeratosis of the stratum corneum and thickening of the stratum corneum. Keratinocyte was proliferated in a large amount, and was arranged in a non-polar manner, the stratum spinosum and stratum basale were thickened, the epidermis was thickened about twice as compared with the Control group rats (table 1), a part of epidermal cells were necrotically disintegrated, melanocytes were proliferated, obvious melanin granules were present, langerhans cells were proliferated, and inflammatory cell infiltration was observed. The sub-dermal local tissue is dark blue and located in the interstitial space. Uneven thickness of collagen fibers. The capillary vessels are dilated and hyperemia is caused, and sebaceous glands are massively proliferated and aggregated, so that the result shows that the skin photoaging model is successfully established. Melanin can absorb UV and reduce DNA mutations in the nucleus, and is a protective measure of the body against UV. In the UVA + lycium barbarum polysaccharide group (figure 1 c), the thickening of the horny layer of the skin is obviously reduced, the thickening is obviously different from that of the UVA group, the necrosis of epidermal cells is reduced, the cells in epidermal acanthocytes and basal lamina cells are only slightly proliferated, the melanin granules are not obvious, and the sebaceous glands are not obviously aggregated, so that the damage of the UVA to the skin is really reduced by the lycium barbarum polysaccharide; compared with the UVA + glycerin group (figure 1 d), the skin stratum corneum also has hyperkeratosis, the thickening degree of the epidermis is not significantly different from that of the UVA group, the hyperplasia of sebaceous glands is accumulated, the blood vessel is dilated and congested, and the melanin particles are obviously consistent with the damage of the UVA group, which shows that the glycerin has no effect of resisting the UVA loss. Therefore, the lycium barbarum polysaccharide has a certain effect of resisting skin UVA damage.

(2) Modification of skin collagen fibers

Changes in collagen fibers of each group were compared by Masson staining. It can be seen that the Control group (fig. 2 a) has uniform skin thickness, clear and no thickening of the spinous layer and the basal layer; the collagen fiber expression amount of the dermis layer is high, the collagen fiber is distributed in a net shape, the thickness is uniform, and no obvious fracture exists; the unprotected UVA group (fig. 2 b) thickened the epidermis and the keratinocytes presented a non-polar arrangement. The local tissue under the dermis is dark blue and is located in the interstitial tissue. The collagen fibril content is reduced. The muscle fibers become wider in cross section. Indicating that UVA has certain damage to collagen fibers of the skin; the form of the collagen fiber of the UVA + lycium barbarum polysaccharide group (figure 2 c) is obviously improved compared with that of the UVA group, the content of the collagen fiber is obviously increased, the breakage degree of the collagen fiber is light, the arrangement is compact, and the damage of the UVA to skin collagen is reduced by lycium barbarum polysaccharide; the damage to collagen fibers was more pronounced in the UVA + glycerol group (fig. 2 d) than in the UVA group, which was disorganized, and it was seen that glycerol did not prevent the effects of UVA on collagen fiber damage. The results show that the lycium barbarum polysaccharide has the effect of resisting UVA damage to collagen fibers.

(3) Altered expression of Caspase-3, an apoptosis factor in skin

The caspase family plays an important role in the execution of apoptosis in mammalian cells. Cleaved Caspase-3 is active Caspase-3 and is the key protease in apoptosis. Firstly, observing the whole section by a low power microscope, and finding that the positive cells in the Control group are lightly stained and few in number and are mainly distributed in sebaceous gland sweat gland cells. While positive cells in the UVA and UVA + glycerol groups stained deeply and were distributed mainly in the epidermal layer. Indicating that UVA irradiation can induce abnormal apoptosis of cells so as to damage the skin. There is less positive cells in the dermis. The IRIDI values of each group are shown in Table 2. The IRIDI values of the Control group and the UVA + lycium barbarum polysaccharide group clear Caspase-3 have no significant difference, but the IRIDI values of the Control group and the UVA + lycium barbarum polysaccharide group are significant difference. Indicating that UVA irradiation can induce abnormal apoptosis of cells so as to damage the skin. The UVA + lycium barbarum polysaccharides group has the effect of reducing apoptosis. There was no significant difference in IRIDI values between the UVA group and the UVA + glycerol group, indicating that glycerol was not protective. The above shows that the skin cell apoptosis caused by UVA can be obviously reduced under the protection of lycium barbarum polysaccharide.

TABLE 2 distribution of positive cells in each group

P1: comparison with Control group P2: comparison with UVA group P3: comparison with UVA + Lycium Bararum polysaccharides group

P values have been corrected by Bonferroni

In conclusion, the lycium barbarum polysaccharide has certain effects of resisting skin UVA damage, resisting UVA damage collagen fibers and reducing skin cell apoptosis caused by UVA, and further the lycium barbarum polysaccharide extract is used as a raw material of the lipstick, so that damage of ultraviolet rays to lips can be reduced, lip wrinkles can be reduced, and the water holding capacity and elasticity of the lips can be ensured.

The steps in the method of the embodiment of the invention can be sequentially adjusted, combined and deleted according to actual needs.

While the invention has been described with reference to a preferred embodiment, it will be understood by those skilled in the art that various changes in form and detail may be made therein without departing from the spirit and scope of the invention.

Claims

1. A medlar lipstick is characterized in that: comprises 5-15 parts of rose essence, 5-20 parts of beeswax, 20-30 parts of wolfberry polysaccharide extract, 10-20 parts of wolfberry red pigment extract, 7-10 parts of olive oil, 7-10 parts of glycerol, 1-5 parts of preservative, 3-6 parts of pearl powder, 2-6 parts of diisostearyl malate and 3-5 parts of edible pigment.

2. A preparation method of a medlar lipstick is characterized by comprising the following steps: the preparation method of the wolfberry lipstick raw material according to claim 1 comprises the following specific steps:

3. The method of making a wolfberry lipstick as claimed in claim 2, wherein: in the first material dissolving step, the first preset temperature is 90-130 ℃.

4. The method of making a wolfberry lipstick as claimed in claim 2, wherein: in the second material dissolving step, the second preset temperature is 70-100 ℃.

5. The method of making a wolfberry lipstick as claimed in claim 2, wherein: in the mixing step, the predetermined temperature gradient is that the first filtrate, the second filtrate, 1-5 parts of preservative, 3-6 parts of pearl essence and 2-6 parts of diisostearyl malate are stirred for a first predetermined time at 80-95 ℃ to obtain a premix, the temperature is reduced to 35-40 ℃, 20-30 parts of lycium barbarum polysaccharide extract and 10-20 parts of lycium barbarum pigment extract are added into the premix and stirred for a second predetermined time to obtain a first mixture.

6. The method of making a wolfberry lipstick as claimed in claim 2, wherein: in the mixing step, the first preset time is 0.8-1.0h, and the second preset time is 0.5-1.0 h.

7. The method of making a wolfberry lipstick as claimed in claim 2, wherein: in the mixing step, the extraction method of the lycium barbarum polysaccharide extract comprises the following steps:

8. The method of making a wolfberry lipstick as claimed in claim 7, wherein: in the step S1: the weight ratio of the medlar powder to the pectinase and the papain is 1: 10-40: 10-40.

9. The method of making a wolfberry lipstick as claimed in claim 2, wherein: in the mixing step, the extraction method of the lycium barbarum red pigment extract comprises the following steps:

and (3) extraction of the medlar red pigment: soxhlet extraction of wolfberry fruit at 85-95 deg.c to obtain red pigment, rotary steaming the extracted liquid until the extracted liquid is concentrated to viscous state, and drying in vacuum drier to obtain red pigment product.

10. The method of making a wolfberry lipstick as claimed in claim 2, wherein: in the filling and forming step, the fourth preset temperature is 70-80 ℃.