CN114540532B - DNA bar code and method for identifying multiple wood in Machilus and Machilus - Google Patents
DNA bar code and method for identifying multiple wood in Machilus and Machilus Download PDFInfo
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Abstract
The invention discloses a DNA bar code and a method for identifying various woods in Nanmu and Machilus, which comprises the DNA bar code formed on the basis of any 2 or all combinations of plastid genome sequences rps16-psbI, psbE-petG, rps8-rpl16, rpl23-ycf2, ndhF-ccsA and ycf 1; and the following steps: s100, grinding a wood sample in a low-temperature environment to obtain a wood powder sample; s200, extracting a DNA sample in the wood powder sample; s300, amplifying ycf1, rps16-psbI and rps8-rpl16 barcode sequences in a plastid genome sequence by using a DNA sample as a template through PCR (polymerase chain reaction) to obtain an amplification product; s400, sequencing the amplified product to obtain sequences of ycf1, rps16-psbI and rps8-rpl16, and combining to obtain a combined barcode of ycf1+ rps16-psbI + rps8-rpl 16; s500, identifying various wood in the Nanmu and the Machilus by adopting a ycf1+ rps16-psbI + rps8-rpl16 combined bar code and based on a Taxon DNA method and a phylogenetic evolution tree method. The DNA bar code has richer sequence information sites, so that the species identification capability is improved; the method is high in identification accuracy, small in sampling amount and suitable for identifying the micro-damage of the high-value wood products.
Description
Technical Field
The invention relates to the field of molecular biology detection of wood species identification, in particular to a DNA bar code and a method for identifying various kinds of wood in Machilus and Machilus.
Background
According to the national standard of China Main Wood name (GB/T18513-2001), nanmu refers to Phoebe sp (Phoebe sp.) wood, which is well known in the world due to the characteristics of precious material, delicate structure, uniform wood color, strong glossiness, good dimensional stability and the like, and is widely applied to ancient buildings such as noble palace, gardens, temple and the like. In modern times, the product forms of nanmu zhu strings, handicraft articles, study rooms, furniture and the like are also diversified. Along with the trend of people toward good life, the demand of the society on nanmu resources is continuously increased, and the nanmu wood resources are increasingly deficient due to illegal and excessive exploitation. Therefore, nanmu resources are gradually receiving attention from international laws and regulations.
At present, nanmu (P.zhennan), phoebe bournei (P.bournei), zhejiang nan (P.chekiangensis) and the like are listed in protection level II of national key protection wild plant directory; meanwhile, several Nanmu genera are also listed in the International Union of Natural protection (IUCN) Red List. Because the wood structural characteristics of the nanmu and the related genus Machilus (Machilus sp.) are very close, high-value nanmu wood products in the wood trade are frequently full of time and fake. Accurate identification of the nanmu species not only provides technical support for effective implementation of important nanmu wood resources and relevant laws and regulations, but also provides important scientific basis for supervision of wood product industrial chains.
The general identification method based on wood anatomy generally has difficulty in identifying the two genera of Machilus and Machilus, and cannot accurately identify the species level, and the DNA molecular marking method provides an effective means for accurately identifying Machilus.
A DNA barcode is a standard, sufficiently variable, easily amplifiable, relatively short segment of DNA. In recent years, DNA barcodes have evolved into an important tool for species identification. However, a single internationally universal DNA barcode, such as rbcL, matK, etc., has a limited ability to identify species, particularly species with complex evolutionary relationships. In order to further improve the accuracy of identifying the nanmu tree species, the invention provides an exclusive DNA barcode and a combined barcode which are preferably suitable for closely related species based on multi-species plastid whole genome comparison, and a DNA barcode identification method is optimized.
Disclosure of Invention
The invention aims to provide a DNA bar code and a method for identifying various wood in Machilus and Machilus, and aims to solve the technical problems of low accuracy and complex method for identifying Machilus tree species in the prior art.
In order to solve the technical problems, the invention specifically provides the following technical scheme:
the invention provides a DNA barcode, which comprises a DNA barcode formed on the basis of any more than 2 or all combinations of plastid genome sequences rps16-psbI, psbE-petG, rps8-rpl16, rpl23-ycf2, ndhF-ccsA and ycf1, and is used for identifying various woods in nanmu and machilus.
As a preferred embodiment of the present invention, the ycf1+ rps16-psbI + rps8-rpl16 combinatorial barcode is shown in SEQ ID No.1-45 as reference for the ycf1+ rps16-psbI + rps8-rpl16 combinatorial barcode.
As a preferable embodiment of the present invention, the plurality of species of wood of the genus Machilus and the genus Machilus are respectively Phoebe bournei, phoma thunbergii p. Chekiangensis, bai Nan p. Neurontha, phoma gracile p. Omeiensis, phoma serrulata p. Sheareri, phoma nanseri p. Zhennan, phoma serrulata Mao Runnan Machilus balansae, phoma huadongensis m.leprophylla, phoma japonica m.pauhoi, phoma serrulata m.thunbergii, and phoma yunnanensis m.yunnanensis.
The invention provides a method for identifying various woods in Machilus and Machilus, which comprises the following steps:
s100, grinding a wood sample at the low temperature of-196 ℃ to prepare a wood powder sample;
s200, extracting a DNA sample in the wood powder sample;
s300, amplifying ycf1, rps16-psbI and rps8-rpl16 barcode sequences in a plastid genome sequence by using the DNA sample as a template through PCR to obtain an amplification product;
s400, sequencing the amplification products to obtain sequences of the ycf1, the rps16-psbI and the rps8-rpl16, and combining the sequences to obtain a combined barcode of the ycf1+ rps16-psbI + rps8-rpl 16;
s500, identifying various wood in the Nanmu and the Machilus by adopting the ycf1+ rps16-psbI + rps8-rpl16 combined bar code and based on a Taxon DNA method and a phylogenetic evolution tree method.
As a preferable aspect of the present invention, the method further includes, in step S100, the steps of:
s101, cutting wood into short wood pieces by using a sterile scalpel, soaking the short wood pieces in sterile 50% ethanol for 1 hour, and performing air drying treatment to obtain an air-dried sample;
s102, adding the air-dried sample and crosslinked polyvinylpyrrolidone powder with the mass ratio of 0.5-1 into a freezing and grinding tube, and mixing and grinding at the low temperature of-196 ℃ to prepare grinding powder;
s103, sieving the ground powder by a sieve of 50-200 meshes to prepare the wood powder sample;
and S104, putting the wood flour sample into a sterilized centrifugal tube for subpackaging and storing.
As a preferable embodiment of the present invention, the method further includes, in step S200, the steps of:
in step S200, the following steps are further included:
s201, adding a rinsing liquid into the wood powder sample, mixing for the first time, rinsing secondary metabolites of xylem of Machilus/Machilus of the wood powder sample by using the rinsing liquid, carrying out centrifugal treatment for 10-15min at 3000-6000 g by using a centrifugal machine after uniformly mixing, and discarding supernatant to obtain first rinsing powder;
s202, adding the rinsing liquid into the first rinsing powder, mixing for the second time, and repeating the centrifuging process in the step S201 to obtain second rinsing powder;
s203, extracting DNA from the second rinsing powder, and purifying the extracted DNA to obtain the DNA sample;
wherein the mixing time of the first mixing and the second mixing is 10min;
the rinsing liquid comprises: 100-300 mM Tris-HCl with pH 8.0, 10-30 mM EDTA with pH 8.0, 1-4% (w/v) PVP-40,5% (v/v) glycerol, 5-10% (w/v) PEG8000 and 300-500 mM sorbitol;
the rinsing liquid is added with 1-2% (v/v) of beta-mercaptoethanol before use.
As a preferable aspect of the present invention, in step S300,
the reaction system for PCR amplification is 0.5-3U DNA polymerase and 1.0-2.0 mM MgCl 2 200 mu M single dNTP, 0.1-5.0 mu M single primer, 0.1-1.5 mg/mL bovine serum albumin with pH of 7.0-8.0 and 10-1000 ng template DNA;
the reaction procedure for the PCR amplification is as follows: pre-denaturation at 94-96 deg.c for 0.5-10 min; denaturation at 94-96 deg.C for 0.05-2min, annealing at 40-65 deg.C for 0.5-2min, extension at 72 deg.C for 0.3-2 min, and circulation for 20-50 times; final extension at 72 deg.c for 2-15 min;
wherein the forward and reverse primers for amplifying the ycf1 sequence are shown in SEQ ID Nos. 46-51, the forward and reverse primers for amplifying the rps16-psbI sequence are shown in SEQ ID Nos. 52-55, and the forward and reverse primers for amplifying the rps8-rpl16 sequence are shown in SEQ ID Nos. 56-59.
As a preferable aspect of the present invention, in step S500,
the Taxon DNA method is based on best close match index judgment;
the phylogenetic evolutionary tree method is based on adjacency method identification.
As a preferred embodiment of the present invention, the index is based on the combined use of 4 indices of nucleotide polymorphism (Pi), DNA fragment acquisition success rate, fragment length and discrimination ability.
As a preferred embodiment of the present invention, the nucleotide polymorphism (Pi) indicator is based on a plastid whole genome sequence alignment and Pi > 0.003;
the success rate of obtaining the DNA fragments is more than or equal to 70 percent;
the length of the fragment is 150-500 bp;
the discriminative power is discriminated based on the Taxon DNA method and the phylogenetic tree method.
Compared with the prior art, the invention has the following beneficial effects:
the method is based on a DNA bar code, has richer sequence information sites, further improves species identification capability, realizes accurate identification of 11 species of Machilus and Machilus woods with extremely similar structural characteristics, and breaks through the limitation that the traditional wood identification technology cannot identify the wood to the seeds; and the optimal selection method of the high-resolution DNA bar codes suitable for identifying the wood of the closely related species is constructed, the identification accuracy is high, the sampling amount is small, and the method is not influenced by the sampling position, the original state of the sample and other factors.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It should be apparent that the drawings in the following description are merely exemplary and that other implementation drawings may be derived from the provided drawings by those of ordinary skill in the art without inventive effort.
FIG. 1 is a schematic flow chart of a preferred method of identifying DNA barcodes for 11 species of Machilus and Machilus wood according to the present invention;
FIG. 2 is a diagram of nucleotide polymorphisms (Pi) of the present invention providing an alignment of Phoebia and Machilus plastid whole genome sequences;
FIG. 3 is a graph showing the success rate of single and combinatorial barcode identification based on Taxon DNA and the neighbor method according to the present invention;
FIG. 4 is a drawing of a phylogenetic tree of Machilus and Machilus constructed based on the adjacency method.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.
As shown in FIGS. 1 to 4, the present invention provides a DNA barcode comprising a DNA barcode formed based on any 2 or more or all combinations of plastid genome sequences rps16-psbI, psbE-petG, rps8-rpl16, rpl23-ycf2, ndhF-ccsA and ycf 1.
Preferably, the DNA barcode is a ycf1+ rps16-psbI + rps8-rpl16 combinatorial barcode. The sequence ycf1+ rps16-psbI + rps8-rpl16 is shown in SEQ ID No.1-45 (see the appendix).
The preferred ycf1+ rps16-psbI + rps8-rpl16 sequence combined DNA bar code has obvious difference sites in 11 wood species of Machilus and Machilus, has strong identification capability, and breaks through the problem of low identification success rate of the universal DNA bar code.
Preferably, the DNA barcode is used to identify 11 species of machilus and machilus wood.
Wherein, the 11 species of Nanmu and Machilus wood are Phoebe bournei, P. Chekiangensis, bai Nan P. Neuranha, P. Meiiensis of Phyllostachys nigra, P. Meierensis of Phoebe, P. Sheareri, P. Zhennan, rust Mao Runnan Machilus balansae, M. Leprophylla, M.pauhoi, M.thunbergii and M.yunnanensis of Phyllostachys yunnanensis, respectively.
The invention provides a method for identifying various woods in Machilus and Machilus, which comprises the following steps:
s100, grinding a wood sample at the low temperature of-196 ℃ to prepare a wood powder sample;
s200, extracting a DNA sample in the wood powder sample;
s300, amplifying barcode sequences of ycf1, rps16-psbI and rps8-rpl16 by using a DNA sample as a template through PCR (polymerase chain reaction) to obtain an amplification product;
s400, sequencing the amplification product to obtain sequences of ycf1, rps16-psbI and rps8-rpl16, and combining the sequences to obtain a combined barcode of ycf1+ rps16-psbI + rps8-rpl 16;
s500, adopting a ycf1+ rps16-psbI + rps8-rpl16 combined bar code, and identifying 11 wood species of Machilus and Machilus based on a Taxon DNA method and a phylogenetic evolution tree method.
The method provides a new thought and technical approach for accurately identifying the nanmu sample, and the screened identifying primer has good specificity and higher amplification and sequencing success rate, and is suitable for DNA materials which are seriously degraded; meanwhile, the TaxonDNA method and the phylogenetic evolutionary tree method are simple to operate, tree species are comprehensively distinguished based on the two methods, and the identification accuracy is high. Because only the powdery sample is needed to extract DNA, the optimal selection method is not influenced by the factors of the material taking positions (boundary wood, boundary wood transition zone and core wood) and the original state of the sample (wood chips and wood powder); and the sampling amount is very small, so that the method is suitable for micro-damage identification.
Preferably, in step S100, the method further includes the steps of:
s101, cutting wood into short wood pieces by using a sterile scalpel, soaking the short wood pieces in sterile 50% ethanol for 1 hour, and then performing air drying treatment to obtain an air-dried sample;
s102, adding an air-dried sample and crosslinked polyvinylpyrrolidone powder in a mass ratio of 0.5-1 into a cryogrinding tube, and mixing and grinding at a low temperature of-196 ℃ to prepare grinding powder;
s103, sieving the ground powder by a sieve with 50-200 meshes to prepare a wood powder sample;
and S104, placing the wood flour sample into a sterilized centrifugal tube for subpackage and preservation.
Preferably, in step S200, the method further includes the steps of:
s201, adding a rinsing liquid into a wood powder sample, mixing for the first time, rinsing secondary metabolites of the xylem of the Machilus/Machilus of the wood powder sample by using the rinsing liquid, carrying out centrifugal treatment for 10-15min at 3000-6000 g by using a centrifugal machine after uniformly mixing, and discarding supernatant to obtain first rinsing powder;
s202, adding rinsing liquid into the first rinsing powder, mixing for the second time, and repeating the centrifuging process in the step S201 to obtain second rinsing powder;
s203, carrying out DNA extraction on the second rinsing powder, and purifying the extracted DNA to prepare a DNA sample;
wherein the mixing time of the first mixing and the second mixing is 10min;
the rinsing liquid comprises: 100-300 mM Tris-HCl with pH 8.0, 10-30 mM EDTA with pH 8.0, 1-4% (w/v) PVP-40,5% (v/v) glycerol, 5-10% (w/v) PEG8000 and 300-500 mM sorbitol;
the rinsing liquid is added with 1-2% (v/v) beta-mercaptoethanol before use.
The invention provides a rinsing method for a wood DNA extraction stage, which is used for effectively removing the inhibiting effect of secondary metabolites rich in oil cells/mucus cells in nanmu wood tissues on DNA extraction.
Preferably, in step S300, the PCR amplification reaction system is 0.5-3U DNA polymerase, 1.0-2.0 mM MgCl 2 200 mu M single dNTP, 0.1-5.0 mu M single primer, 0.1-1.5 mg/mL bovine serum albumin with pH of 7.0-8.0 and 10-1000 ng template DNA. The PCR amplification reaction conditions were as follows: pre-denaturation at 94-96 deg.c for 0.5-10 min; denaturation at 94-96 deg.C for 0.05-2min, annealing at 40-65 deg.C for 0.5-2min, extension at 72 deg.C for 0.3-2 min, and circulation for 20-50 times; and final extension at 72 deg.c for 2-15 min.
Wherein, the positive and negative primers for amplifying the ycf1 sequence are shown in SEQ ID No.46-51, the positive and negative primers for amplifying the rps16-psbI sequence are shown in SEQ ID No.52-55, and the positive and negative primers for amplifying the rps8-rpl16 sequence are shown in SEQ ID No. 56-59.
The identification primer screened by the invention has good specificity and higher amplification and sequencing success rate, and is suitable for wood materials which are subjected to deep processing such as high-temperature drying and coating or are stored for a long time and have DNA seriously degraded.
Preferably, in step S500, the taxon dna method is identified based on the "best close match" index, and the phylogenetic tree method is identified based on the Neighbor-join method (NJ). The TaxonDNA method and the phylogenetic evolutionary tree method are simple to operate, tree species are comprehensively distinguished based on the two methods, and the identification accuracy is high.
The preferable method for identifying the DNA barcodes of various Machilus and Machilus woods is the combined application of 4 indexes of nucleotide polymorphism (Pi), DNA fragment acquisition success rate, fragment length and identification capacity. The comprehensive indexes of nucleotide polymorphism (Pi), acquisition success rate, fragment length, identification capability and the like are used as the optimized standard of the wood DNA bar code, so that the identification accuracy is effectively improved, and the identification difficulty is reduced.
The nucleotide polymorphism (Pi) index is based on the comparison of plastid whole genome sequences, pi is more than 0.003, the DNA fragment acquisition success rate is more than or equal to 70 percent, the fragment length is 150-500 bp, and the discrimination capability is based on the discrimination of a Taxon DNA method and a phylogenetic tree method.
The experimental conditions of the experimental steps are less dependent, and can be met by common PCR laboratories.
The present invention constructs a DNA barcode, and the above scheme is explained as follows:
example 1
1. Phoebe/Machilus tree species wood plastid whole genome horizontal screening DNA hypervariable region
(1) Plasmid DNA whole genome sequences of Machilus/Machilus were downloaded from GenBank (Table 1);
TABLE 1 Photinia/Machilus plastid DNA genome information
| Name of tree species | GenBank accession number |
| Machilus yunnanensis | KT348516 |
| Machilus thunbergii | MH178404 |
| Machilus pauhoi | MH178403 |
| Machilus balansae | KT348517 |
| Phoebe bournei | KY346512 |
| Phoebe omeiensis | KX437772 |
| Phoebe zhennan | MH033832 |
| Phoebe chekiangensis | KY346511 |
| Phoebe sheareri | KX437773 |
(2) Performing the whole plastid genome sequence alignment by using MAFFT software;
(3) 6 DNA hypervariable regions such as rps16-psbI, psbE-petG, rps8-rpl16, rpl23-ycf2, ndhF-ccsA and ycf1 were selected as Nanmu candidate DNA barcodes based on nucleotide polymorphism index (Pi > 0.004) by using DnaSP 5.0 software (FIG. 2, table 2).
TABLE 2 NAMULIS PLANTINUM GENE DNA hypermutable region characteristics
2. Machilus/Machilus tree species wood DNA hypervariable region primer design
(1) 6 DNA hypervariable region sequences screened from the Nana and the Machilus species are respectively compared by BioEdit software, and the region of each sequence with an information site is determined.
(2) Primers were designed by Primer Premier 5 software, and the length of the target fragment was 300 to 500bp (Table 3).
TABLE 3 DNA hypervariable region sequence primer information
The invention constructs a method for identifying various woods in Machilus and Machilus, and the scheme is described by taking various wood samples as an example and combining specific embodiments.
Example 2
DNA identification of Nanmu/Machilus air-dried material sample
(1) 1 artificial forest log air-dried wood sample of nominal Zhejiang nan is identified as a Nanmu/Machilus wood through anatomical structure, but specific tree species of Nanmu or Machilus cannot be judged. The wood sample is thus identified based on the DNA method.
(2) Preparation of Wood samples
Selecting a wood sample, and cutting the surface of 1-2 mm of wood by using a scalpel treated by a nucleic acid pollution scavenger so as to avoid exogenous pollution; cutting off a plurality of wood chips from a wood sample, soaking the wood chips in a 50% ethanol solution for 1 hour, and then air-drying;
then transferring the sawdust into a grinding tube, adding crosslinked polyvinylpyrrolidone with the weight of 1/2 of the sample, placing the grinding tube in a freezing grinder for precooling for 2min at low temperature, grinding for 2min, cooling for 1min, grinding for 2min, and operating the frequency of 12cps; after grinding, the wood powder is subpackaged into 50ml sterile centrifuge tubes, the amount of the wood powder in each tube is 750mg, and the tubes are stored in a low-temperature refrigerator at minus 80 ℃ for later use.
(3) Wood DNA extraction
After wood flour was washed 3 times with a rinsing solution in a pre-treated ultra-clean working environment, 500mg of wood flour was subjected to DNA extraction.
(4) PCR amplification reaction and sequencing
Performing PCR amplification by using the wood DNA extract as a template, wherein amplification primers are shown as SEQ ID 46-59. The reaction system is 30 μ L: among them, premix Ex Taq 15. Mu.L (including l.25U Ex Taq DNA polymerase, 2mM MgCl2, 200. Mu.M single dNTP), 0.2. Mu.M single primer and about 10ng template DNA.
All PCR reactions were performed on a PCR amplificator.
The reaction procedure is as follows: pre-denaturation at 94 ℃ for 90s; denaturation at 94 ℃ 30s,45 ℃ (ycf 1), 45 ℃ (rps 16-psbI) and 46 ℃ (rps 8-rpl 16) were annealed for 20s, extension at 72 ℃ for 30s, respectively, and the cycle was 40 times; and finally extending for 7min at 72 ℃ to obtain the target DNA fragment amplified efficiently.
And purifying the amplified product and then performing bidirectional direct sequencing.
(5) Sequence alignment analysis and tree species identification
And combining the sequencing results of three sequences of ycf1, rps16-psbI and rps8-rpl16, and constructing a phylogenetic tree by adopting a combined sequence of ycf1+ rps16-psbI + rps8-rpl 16. The analysis result shows that the sample can be clustered with a Phoebe chekiangensis (sequence accession number KY 346511) sample published by GenBank (figure 3), and is distinguished from other Phoebe/Phoebe tree species.
The clustering result proves that the sample is Phoebe chekiangensis P.
Example 3
Naming Nanna/Machilus wood specimens
(1) 2 wood samples which are respectively named as Phoebe sp and Machilus sp from the institute of Wood industry of China forestry science research institute are identified as the wood of the Machilus/Machilus through the anatomical structure of the wood and the comprehensive sample information, but specific tree species of the Machilus or the Machilus cannot be judged. Thus, wood specimens were named based on the DNA method.
(2) Preparing wood flour and grinding to 200 meshes or above.
(3) Extracting DNA of wood:
DNA extraction was performed on wood in a pre-treated ultra-clean working environment. The DNA extraction stock solution was further purified by a purification Kit (High Pure PCR Template Kit, roche, germany) to a final DNA concentration of 1-200 ng/. Mu.L.
(4) PCR amplification reaction and sequencing
The above-mentioned DNA stock was amplified and sequenced with reference to the PCR amplification system and sequencing method of example 2.
(5) Sequence alignment analysis and tree species identification
Combining the sequencing results of three sequences of ycf1, rps16-psbI and rps8-rpl16, and constructing a phylogenetic tree by adopting a combined sequence of ycf1+ rps16-psbI + rps8-rpl 16. The analysis result shows that the product is nominally Phoebe sp and can be clustered with a Phoebe P.sheareri (sequence accession number KX 437773) sample published by GenBank; nominally Machilus sp. Was able to cluster with Machilus pauhoi (sequence accession number MH 178403) samples published in GenBank (fig. 3), but was distinguished from other nanmu/Machilus tree species.
The clustering result proves that 2 wood samples are respectively the p.
Example 4
Nanmu structure building sample DNA identification
(1) The 3 samples which are respectively from the wood components of the wood structure building in the Beijing area and are nominally nanmu are identified as nanmu/machilus wood through anatomical structures, but specific tree species of the nanmu or the machilus cannot be judged. Therefore, the tree species information of the wood member sample for construction is identified based on the DNA method.
(2) In an ultra-clean working environment with UV irradiation for 48 hours, a sample is placed in a 3% sodium hypochlorite solution for soaking for 30min, and then a scalpel processed by a nucleic acid pollution scavenger is used for cutting the surface of wood with the thickness of 1-2 mm so as to avoid exogenous pollution;
cutting off a plurality of sawdust from a wood sample, placing in a cryogrinding instrument for precooling for 2min at low temperature, grinding for 2min, cooling for 1min, grinding for 2min, and operating at the frequency of 12cps; after grinding, the fine wood powder is subpackaged into 2ml of micro sterile centrifuge tubes, the amount of the wood powder in each tube is 100mg, and the tubes are stored in a low-temperature refrigerator at minus 80 ℃ for later use.
(3) Wood DNA extraction
In a pre-treated ultra-clean working environment, the DNA extraction method of example 2 was referenced, while 3 parts of 100mg of wood flour were subjected to DNA extraction.
3 DNA extracts were further purified and concentrated using a purification Kit (DNeasy Powerclean Pro clean Kit, qiagen, germany) to increase the DNA concentration to a final concentration of 0.04-2 ng/. Mu.L.
(4) PCR amplification reaction and sequencing
The above-mentioned DNA stock was amplified and sequenced with reference to the PCR amplification system and the sequencing method in reference example 2.
(5) Sequence alignment analysis and tree species identification
And combining the sequencing results of three sequences of ycf1, rps16-psbI and rps8-rpl16, and constructing a phylogenetic tree by adopting a combined sequence of ycf1+ rps16-psbI + rps8-rpl 16. The analysis result shows that 3 wood structure building samples which are nominal as nanmu can be clustered with a nanmu P.zhennan sample sequence published by GenBank and are distinguished from other nanmu/machina tree species.
The clustering result proves that all 3 wood structure building samples with nominal nanmu are P.zhennan.
As shown in the above embodiments, the DNA barcode and the method for identifying multiple kinds of wood in machilus and machilus provided by the present invention have the following advantages:
(1) Through two combinations and three combinations of 6 DNA fragments including rps16-psbI, psbE-petG, rps8-rpl16, rpl23-ycf2, ndhF-ccsA and ycf1, the combination of ycf1+ rps16-psbI + rps8-rpl16, ycf1+ ndhF-ccsA + rpl23-ycf2 and ycf1+ ndhF-ccsA + rps8-rpl16 has DNA coding gap, and the DNA coding gap can be used as a high-resolution DNA bar code for identifying 11 species of genus/Machilus wood;
(2) Aiming at samples in different preservation states such as fresh wood, air-dried wood, wood samples and long-term storage wood, the wood DNA acquisition success rate (PCR amplification success rate and sequencing success rate) is high, the wood DNA acquisition success rate provided by the invention is more than 70%, the sample sampling amount is small, and the method belongs to micro-damage identification for high-value wood products;
(3) The highest success rate of species identification was obtained with the DNA combinatorial barcode ycf1+ rps16-psbI + rps8-rpl16 as assessed by both the Taxon DNA and the neighbor joining methods (as shown in FIG. 3).
By the DNA bar code and the method for identifying various woods in the Machilus and the Machilus, a high-resolution DNA bar code and a method for acquiring DNA of the woods are provided, and an identification method between a DNA sequence of the woods and a reference sequence is provided, so that the accurate identification of the 11 kinds of woods of the Machilus and the Machilus is realized, the success rate of DNA acquisition and the accuracy of the woods of the Machilus and the Machilus are improved, the problem that the woods cannot be identified to the seed level by a traditional wood identification method is solved, an efficient and reliable identification method is provided for the fields of quality inspection and quarantine of high-value wood products and operation and trade of the woods, and the method has high practical value.
The above embodiments are only exemplary embodiments of the present application, and are not intended to limit the present application, and the protection scope of the present application is defined by the claims. Various modifications and equivalents may be made by those skilled in the art within the spirit and scope of the present application and such modifications and equivalents should also be considered to be within the scope of the present application.
Sequence listing
<110> institute of Wood industry of China institute of forestry science and research
<120> DNA bar code and method for identifying various wood in Machilus and Machilus
<141> 2022-02-20
<160> 59
<170> SIPOSequenceListing 1.0
<210> 1
<211> 2700
<212> DNA
<213> Phoebe zhennan
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ctacttcaat atcgcgaagc atatcttatt cttaaggaaa gaatcaggaa tttttttgga 240
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aatatatcca ttttatgttc aagtttttgg taatcagtag gaagcatatc atgaatctta 1020
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agcaacaata acgtcaccaa tatgagcata tcgttgatta ctagctccta tgattcgaat 1740
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ccattcagcg tatgttcgcc ccctcgccca tattcatatt gaaggttagt tagtcatttg 1920
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agcaacaata acgtcaccaa tatgagcata tcgttgatta ctagctccta tgattcgaat 1740
acacatcaat tctcgagccc cgctgttgtc cgctacattc aaatgggtct gaggttgaat 1800
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ccattcagcg tatgttcgcc ccctcgccca tattcatatt gaaggttagt tagtcatttg 1920
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aaatgcgaaa taaaagagaa gtctctacct tccctaaagt aaatataaat aaagataggg 2040
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tcccataaga agatcctttt aaattggctt tggatatcat tcatgatccc cagggcagaa 2280
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ttcattttga ttctacatct acatgtgtag aatcatagca acaaccaatt caatagatat 2580
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<210> 3
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<212> DNA
<213> Phoebe zhennan
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ctacttcaat atcgcgaagc atatcttatt cttaaggaaa gaatcaggaa tttttttgga 240
acacgaagaa tatttgattc caaatcaagg cataaaaaac ttcagaattc tggaatgaat 300
gaatggaaaa actggttaag gggtcattat caatacaatt tatctcagga tttatcgata 360
atcttttttg ttgattcaag gaaaagttgt acattgactc tagaaatatt aacagtactt 420
agaaagatat gtatgtatat tctttcgttg aaaaatgtca aaaaatagtg ccatttgcgt 480
atgaatatga atctgttact tcttcttttt gatatctgcc aaatgggttt ctgcgattcc 540
gatcttttat caccacaact tgtatcgtta ggactaatat ttatatccgg agttagaaat 600
atttttcttt tgtcttttgc acccctttct atttgatttc tgattagggt tgttctatcg 660
ttttcctcct cccgctcttc aaaagaatcg atcaatttgt atgatcgtcg aggaatcttt 720
ttaccgattt cttctattcc aatagatttt ttttgaattg tttgattatt tggatcagtt 780
gtaattacat cgaatagaaa tttcaaacat tttgttttat tttctgaatc aaatcttctt 840
tgttcggata ttaaaacaag ctttttaaaa ttaagattaa aaccagttgt tgatttccta 900
gctaattcac tgatagaggt caagaaagga ccaatgtctg tcgataatga ttctccatta 960
aatatatcca ttttatgttc aagtttttgg taatcagtag gaagcatatc atgaatctta 1020
tttatccaaa ccattcctat agaatcttct gtccccattc cacctaaaat cctaggaatt 1080
cgttgatggt tggaatagat tcgtagaccg ggacgactga tacgctttaa aatatttcta 1140
tatgttcctt tcctattcct tctatgtcgc agggttgaaa ccaaaaaata tttgttgttt 1200
tccctatgtt tcctaacatt ttcaataaac ccttcttgta gaagtatttt aacaatgttt 1260
tcggcgatat tagtagatgc tattcgaacc gttccttttt tatccatgtt agcatttctt 1320
atagaagtta ttatatcggc aatagtgtcc ctacccatga cgaactaaaa ttatgggtgc 1380
cttccagttt tgatataatc aacatgttac tttttttttt tcatctacta gtatttataa 1440
gacttcagga gctaatgaga ctattttagt gaaattcaac tgtctcaatt cccgcgcgat 1500
cgctccaaaa actcgagttc cttttggatt tccttcttga tcaatgacaa ctgctgcatt 1560
gtcatcatat cgtattatca taccgttgtc gcgcttgagt tctttacatg tacgtacaat 1620
tacagctctg atcacttctg atctttcgag aggcatattg ggcactgctt ctttgattac 1680
agcaacaata acgtcaccaa tatgagcata tcgttgatta ctagctccta tgattcgaat 1740
acacatcaat tctcgagccc cgctgttgtc cgctacattc aaatgggtct gaggttgaat 1800
catatcattt tttttttaac aaagatcaaa gtgaaattca aaaaaaaaaa actgggtggg 1860
ccattcagcg tatgttcgcc ccctcgccca tattcatatt gaaggttagt tagtcatttg 1920
gataaacttt atgccccata tctatgatat ttggattctc acatgatgca ttctgagtcg 1980
aaatgcgaaa taaaagagaa gtctctacct tccctaaagt aaatataaat aaagataggg 2040
tagacaaaat gactaattct atgtggatcc tgaatcctaa aaaacagggg ggttcttggt 2100
attaattcca tcgctggaat taaagctcct gagactgggg tgggacaaaa tcaaacaaaa 2160
tagtaacaac gaattgatat gaacccattt tgctttgtac ttatacaaga caggatagca 2220
tcccataaga agatcctttt aaattggctt tggatatcat tcatgatccc cagggcagaa 2280
ttaagacaga tcaaactaaa gatattaagc ataacaaaca ttttgttctt ggagaaaatt 2340
tcattattat tgggttattg atataagggg aaaatgaaga aagaagggaa agtaggccgc 2400
aaaatctttc tttttctttg aactccctca atcaaaaatc cttcaattct caaatgagaa 2460
tagaaggaat cataccttac atacaatatc ttaattgaat tatatgtaat gatcaataaa 2520
ttcattttga ttctacatct acatgtgtag aatcatagca acaaccaatt caatagatat 2580
tggggctgga attgacgaac aaccaatacc gatattagtg tttatcggtg tcgaatagaa 2640
ataaaaatgg ggcgtggcca agtggtaagg caacgggttt tggtcccgtt actcggaggt 2700
<210> 4
<211> 2700
<212> DNA
<213> Phoebe zhennan
<400> 4
gaagggattg atgaaacaaa tcaaaaaaaa attcacttta tttcgactat aaaaaagtct 60
ctttctaata ttagtaataa taaatcaaag atttctggtg acctatattc cttttcacaa 120
gcatctgtat tttacaaatt atcacaaatc caagctatta agaagtatca tttgagatct 180
ctacttcaat atcgcgaagc atatcttatt cttaaggaaa gaatcaggaa tttttttgga 240
acacgaagaa tatttgattc caaatcaagg cataaaaaac ttcagaattc tggaatgaat 300
gaatggaaaa actggttaag gggtcattat caatacaatt tatctcagga tttatcgata 360
atcttttttg ttgattcaag gaaaagttgt acattgactc tagaaatatt aacagtactt 420
agaaagatat gtatgtatat tctttcgttg aaaaatgtca aaaaatagtg ccatttgcgt 480
atgaatatga atctgttact tcttcttttt gatatctgcc aaatgggttt ctgcgattcc 540
gatcttttat caccacaact tgtatcgtta ggactaatat ttatatccgg agttagaaat 600
atttttcttt tgtcttttgc acccctttct atttgatttc tgattagggt tgttctatcg 660
ttttcctcct cccgctcttc aaaagaatcg atcaatttgt atgatcgtcg aggaatcttt 720
ttaccgattt cttctattcc aatagatttt ttttgaattg tttgattatt tggatcagtt 780
gtaattacat cgaatagaaa tttcaaacat tttgttttat tttctgaatc aaatcttctt 840
tgttcggata ttaaaacaag ctttttaaaa ttaagattaa aaccagttgt tgatttccta 900
gctaattcac tgatagaggt caagaaagga ccaatgtctg tcgataatga ttctccatta 960
aatatatcca ttttatgttc aagtttttgg taatcagtag gaagcatatc atgaatctta 1020
tttatccaaa ccattcctat agaatcttct gtccccattc cacctaaaat cctaggaatt 1080
cgttgatggt tggaatagat tcgtagaccg ggacgactga tacgctttaa aatatttcta 1140
tatgttcctt tcctattcct tctatgtcgc agggttgaaa ccaaaaaata tttgttgttt 1200
tccctatgtt tcctaacatt ttcaataaac ccttcttgta gaagtatttt aacaatgttt 1260
tcggcgatat tagtagatgc tattcgaacc gttccttttt tatccatgtt agcatttctt 1320
atagaagtta ttatatcggc aatagtgtcc ctacccatga cgaactaaaa ttatgggtgc 1380
cttccagttt tgatataatc aacatgttac tttttttttt tcatctacta gtatttataa 1440
gacttcagga gctaatgaga ctattttagt gaaattcaac tgtctcaatt cccgcgcgat 1500
cgctccaaaa actcgagttc cttttggatt tccttcttga tcaatgacaa ctgctgcatt 1560
gtcatcatat cgtattatca taccgttgtc gcgcttgagt tctttacatg tacgtacaat 1620
tacagctctg atcacttctg atctttcgag aggcatattg ggcactgctt ctttgattac 1680
agcaacaata acgtcaccaa tatgagcata tcgttgatta ctagctccta tgattcgaat 1740
acacatcaat tctcgagccc cgctgttgtc cgctacattc aaatgggtct gaggttgaat 1800
catatcattt tttttttaac aaagatcaaa gtgaaattca aaaaaaaaaa actgggtggg 1860
ccattcagcg tatgttcgcc ccctcgccca tattcatatt gaaggttagt tagtcatttg 1920
gataaacttt atgccccata tctatgatat ttggattctc acatgatgca ttctgagtcg 1980
aaatgcgaaa taaaagagaa gtctctacct tccctaaagt aaatataaat aaagataggg 2040
tagacaaaat gactaattct atgtggatcc tgaatcctaa aaaacagggg ggttcttggt 2100
attaattcca tcgctggaat taaagctcct gagactgggg tgggacaaaa tcaaacaaaa 2160
tagtaacaac gaattgatat gaacccattt tgctttgtac ttatacaaga caggatagca 2220
tcccataaga agatcctttt aaattggctt tggatatcat tcatgatccc cagggcagaa 2280
ttaagacaga tcaaactaaa gatattaagc ataacaaaca ttttgttctt ggagaaaatt 2340
tcattattat tgggttattg atataagggg aaaatgaaga aagaagggaa agtaggccgc 2400
aaaatctttc tttttctttg aactccctca atcaaaaatc cttcaattct caaatgagaa 2460
tagaaggaat cataccttac atacaatatc ttaattgaat tatatgtaat gatcaataaa 2520
ttcattttga ttctacatct acatgtgtag aatcatagca acaaccaatt caatagatat 2580
tggggctgga attgacgaac aaccaatacc gatattagtg tttatcggtg tcgaatagaa 2640
ataaaaatgg ggcgtggcca agtggtaagg caacgggttt tggtcccgtt actcggaggt 2700
<210> 5
<211> 2700
<212> DNA
<213> Phoebe zhennan
<400> 5
gaagggattg atgaaacaaa tcaaaaaaaa attcacttta tttcgactat aaaaaagtct 60
ctttctaata ttagtaataa taaatcaaag atttctggtg acctatattc cttttcacaa 120
gcatctgtat tttacaaatt atcacaaatc caagctatta agaagtatca tttgagatct 180
ctacttcaat atcgcgaagc atatcttatt cttaaggaaa gaatcaggaa tttttttgga 240
acacgaagaa tatttgattc caaatcaagg cataaaaaac ttcagaattc tggaatgaat 300
gaatggaaaa actggttaag gggtcattat caatacaatt tatctcagga tttatcgata 360
atcttttttg ttgattcaag gaaaagttgt acattgactc tagaaatatt aacagtactt 420
agaaagatat gtatgtatat tctttcgttg aaaaatgtca aaaaatagtg ccatttgcgt 480
atgaatatga atctgttact tcttcttttt gatatctgcc aaatgggttt ctgcgattcc 540
gatcttttat caccacaact tgtatcgtta ggactaatat ttatatccgg agttagaaat 600
atttttcttt tgtcttttgc acccctttct atttgatttc tgattagggt tgttctatcg 660
ttttcctcct cccgctcttc aaaagaatcg atcaatttgt atgatcgtcg aggaatcttt 720
ttaccgattt cttctattcc aatagatttt ttttgaattg tttgattatt tggatcagtt 780
gtaattacat cgaatagaaa tttcaaacat tttgttttat tttctgaatc aaatcttctt 840
tgttcggata ttaaaacaag ctttttaaaa ttaagattaa aaccagttgt tgatttccta 900
gctaattcac tgatagaggt caagaaagga ccaatgtctg tcgataatga ttctccatta 960
aatatatcca ttttatgttc aagtttttgg taatcagtag gaagcatatc atgaatctta 1020
tttatccaaa ccattcctat agaatcttct gtccccattc cacctaaaat cctaggaatt 1080
cgttgatggt tggaatagat tcgtagaccg ggacgactga tacgctttaa aatatttcta 1140
tatgttcctt tcctattcct tctatgtcgc agggttgaaa ccaaaaaata tttgttgttt 1200
tccctatgtt tcctaacatt ttcaataaac ccttcttgta gaagtatttt aacaatgttt 1260
tcggcgatat tagtagatgc tattcgaacc gttccttttt tatccatgtt agcatttctt 1320
atagaagtta ttatatcggc aatagtgtcc ctacccatga cgaactaaaa ttatgggtgc 1380
cttccagttt tgatataatc aacatgttac tttttttttt tcatctacta gtatttataa 1440
gacttcagga gctaatgaga ctattttagt gaaattcaac tgtctcaatt cccgcgcgat 1500
cgctccaaaa actcgagttc cttttggatt tccttcttga tcaatgacaa ctgctgcatt 1560
gtcatcatat cgtattatca taccgttgtc gcgcttgagt tctttacatg tacgtacaat 1620
tacagctctg atcacttctg atctttcgag aggcatattg ggcactgctt ctttgattac 1680
agcaacaata acgtcaccaa tatgagcata tcgttgatta ctagctccta tgattcgaat 1740
acacatcaat tctcgagccc cgctgttgtc cgctacattc aaatgggtct gaggttgaat 1800
catatcattt tttttttaac aaagatcaaa gtgaaattca aaaaaaaaaa actgggtggg 1860
ccattcagcg tatgttcgcc ccctcgccca tattcatatt gaaggttagt tagtcatttg 1920
gataaacttt atgccccata tctatgatat ttggattctc acatgatgca ttctgagtcg 1980
aaatgcgaaa taaaagagaa gtctctacct tccctaaagt aaatataaat aaagataggg 2040
tagacaaaat gactaattct atgtggatcc tgaatcctaa aaaacagggg ggttcttggt 2100
attaattcca tcgctggaat taaagctcct gagactgggg tgggacaaaa tcaaacaaaa 2160
tagtaacaac gaattgatat gaacccattt tgctttgtac ttatacaaga caggatagca 2220
tcccataaga agatcctttt aaattggctt tggatatcat tcatgatccc cagggcagaa 2280
ttaagacaga tcaaactaaa gatattaagc ataacaaaca ttttgttctt ggagaaaatt 2340
tcattattat tgggttattg atataagggg aaaatgaaga aagaagggaa agtaggccgc 2400
aaaatctttc tttttctttg aactccctca atcaaaaatc cttcaattct caaatgagaa 2460
tagaaggaat cataccttac atacaatatc ttaattgaat tatatgtaat gatcaataaa 2520
ttcattttga ttctacatct acatgtgtag aatcatagca acaaccaatt caatagatat 2580
tggggctgga attgacgaac aaccaatacc gatattagtg tttatcggtg tcgaatagaa 2640
ataaaaatgg ggcgtggcca agtggtaagg caacgggttt tggtcccgtt actcggaggt 2700
<210> 6
<211> 2700
<212> DNA
<213> Phoebe zhennan
<400> 6
gaagggattg atgaaacaaa tcaaaaaaaa attcacttta tttcgactat aaaaaagtct 60
ctttctaata ttagtaataa taaatcaaag atttctggtg acctatattc cttttcacaa 120
gcatctgtat tttacaaatt atcacaaatc caagctatta agaagtatca tttgagatct 180
ctacttcaat atcgcgaagc atatcttatt cttaaggaaa gaatcaggaa tttttttgga 240
acacgaagaa tatttgattc caaatcaagg cataaaaaac ttcagaattc tggaatgaat 300
gaatggaaaa actggttaag gggtcattat caatacaatt tatctcagga tttatcgata 360
atcttttttg ttgattcaag gaaaagttgt acattgactc tagaaatatt aacagtactt 420
agaaagatat gtatgtatat tctttcgttg aaaaatgtca aaaaatagtg ccatttgcgt 480
atgaatatga atctgttact tcttcttttt gatatctgcc aaatgggttt ctgcgattcc 540
gatcttttat caccacaact tgtatcgtta ggactaatat ttatatccgg agttagaaat 600
atttttcttt tgtcttttgc acccctttct atttgatttc tgattagggt tgttctatcg 660
ttttcctcct cccgctcttc aaaagaatcg atcaatttgt atgatcgtcg aggaatcttt 720
ttaccgattt cttctattcc aatagatttt ttttgaattg tttgattatt tggatcagtt 780
gtaattacat cgaatagaaa tttcaaacat tttgttttat tttctgaatc aaatcttctt 840
tgttcggata ttaaaacaag ctttttaaaa ttaagattaa aaccagttgt tgatttccta 900
gctaattcac tgatagaggt caagaaagga ccaatgtctg tcgataatga ttctccatta 960
aatatatcca ttttatgttc aagtttttgg taatcagtag gaagcatatc atgaatctta 1020
tttatccaaa ccattcctat agaatcttct gtccccattc cacctaaaat cctaggaatt 1080
cgttgatggt tggaatagat tcgtagaccg ggacgactga tacgctttaa aatatttcta 1140
tatgttcctt tcctattcct tctatgtcgc agggttgaaa ccaaaaaata tttgttgttt 1200
tccctatgtt tcctaacatt ttcaataaac ccttcttgta gaagtatttt aacaatgttt 1260
tcggcgatat tagtagatgc tattcgaacc gttccttttt tatccatgtt agcatttctt 1320
atagaagtta ttatatcggc aatagtgtcc ctacccatga cgaactaaaa ttatgggtgc 1380
cttccagttt tgatataatc aacatgttac tttttttttt tcatctacta gtatttataa 1440
gacttcagga gctaatgaga ctattttagt gaaattcaac tgtctcaatt cccgcgcgat 1500
cgctccaaaa actcgagttc cttttggatt tccttcttga tcaatgacaa ctgctgcatt 1560
gtcatcatat cgtattatca taccgttgtc gcgcttgagt tctttacatg tacgtacaat 1620
tacagctctg atcacttctg atctttcgag aggcatattg ggcactgctt ctttgattac 1680
agcaacaata acgtcaccaa tatgagcata tcgttgatta ctagctccta tgattcgaat 1740
acacatcaat tctcgagccc cgctgttgtc cgctacattc aaatgggtct gaggttgaat 1800
catatcattt tttttttaac aaagatcaaa gtgaaattca aaaaaaaaaa actgggtggg 1860
ccattcagcg tatgttcgcc ccctcgccca tattcatatt gaaggttagt tagtcatttg 1920
gataaacttt atgccccata tctatgatat ttggattctc acatgatgca ttctgagtcg 1980
aaatgcgaaa taaaagagaa gtctctacct tccctaaagt aaatataaat aaagataggg 2040
tagacaaaat gactaattct atgtggatcc tgaatcctaa aaaacagggg ggttcttggt 2100
attaattcca tcgctggaat taaagctcct gagactgggg tgggacaaaa tcaaacaaaa 2160
tagtaacaac gaattgatat gaacccattt tgctttgtac ttatacaaga caggatagca 2220
tcccataaga agatcctttt aaattggctt tggatatcat tcatgatccc cagggcagaa 2280
ttaagacaga tcaaactaaa gatattaagc ataacaaaca ttttgttctt ggagaaaatt 2340
tcattattat tgggttattg atataagggg aaaatgaaga aagaagggaa agtaggccgc 2400
aaaatctttc tttttctttg aactccctca atcaaaaatc cttcaattct caaatgagaa 2460
tagaaggaat cataccttac atacaatatc ttaattgaat tatatgtaat gatcaataaa 2520
ttcattttga ttctacatct acatgtgtag aatcatagca acaaccaatt caatagatat 2580
tggggctgga attgacgaac aaccaatacc gatattagtg tttatcggtg tcgaatagaa 2640
ataaaaatgg ggcgtggcca agtggtaagg caacgggttt tggtcccgtt actcggaggt 2700
<210> 7
<211> 2700
<212> DNA
<213> Phoebe zhennan
<400> 7
gaagggattg atgaaacaaa tcaaaaaaaa attcacttta tttcgactat aaaaaagtct 60
ctttctaata ttagtaataa taaatcaaag atttctggtg acctatattc cttttcacaa 120
gcatctgtat tttacaaatt atcacaaatc caagctatta agaagtatca tttgagatct 180
ctacttcaat atcgcgaagc atatcttatt cttaaggaaa gaatcaggaa tttttttgga 240
acacgaagaa tatttgattc caaatcaagg cataaaaaac ttcagaattc tggaatgaat 300
gaatggaaaa actggttaag gggtcattat caatacaatt tatctcagga tttatcgata 360
atcttttttg ttgattcaag gaaaagttgt acattgactc tagaaatatt aacagtactt 420
agaaagatat gtatgtatat tctttcgttg aaaaatgtca aaaaatagtg ccatttgcgt 480
atgaatatga atctgttact tcttcttttt gatatctgcc aaatgggttt ctgcgattcc 540
gatcttttat caccacaact tgtatcgtta ggactaatat ttatatccgg agttagaaat 600
atttttcttt tgtcttttgc acccctttct atttgatttc tgattagggt tgttctatcg 660
ttttcctcct cccgctcttc aaaagaatcg atcaatttgt atgatcgtcg aggaatcttt 720
ttaccgattt cttctattcc aatagatttt ttttgaattg tttgattatt tggatcagtt 780
gtaattacat cgaatagaaa tttcaaacat tttgttttat tttctgaatc aaatcttctt 840
tgttcggata ttaaaacaag ctttttaaaa ttaagattaa aaccagttgt tgatttccta 900
gctaattcac tgatagaggt caagaaagga ccaatgtctg tcgataatga ttctccatta 960
aatatatcca ttttatgttc aagtttttgg taatcagtag gaagcatatc atgaatctta 1020
tttatccaaa ccattcctat agaatcttct gtccccattc cacctaaaat cctaggaatt 1080
cgttgatggt tggaatagat tcgtagaccg ggacgactga tacgctttaa aatatttcta 1140
tatgttcctt tcctattcct tctatgtcgc agggttgaaa ccaaaaaata tttgttgttt 1200
tccctatgtt tcctaacatt ttcaataaac ccttcttgta gaagtatttt aacaatgttt 1260
tcggcgatat tagtagatgc tattcgaacc gttccttttt tatccatgtt agcatttctt 1320
atagaagtta ttatatcggc aatagtgtcc ctacccatga cgaactaaaa ttatgggtgc 1380
cttccagttt tgatataatc aacatgttac tttttttttt tcatctacta gtatttataa 1440
gacttcagga gctaatgaga ctattttagt gaaattcaac tgtctcaatt cccgcgcgat 1500
cgctccaaaa actcgagttc cttttggatt tccttcttga tcaatgacaa ctgctgcatt 1560
gtcatcatat cgtattatca taccgttgtc gcgcttgagt tctttacatg tacgtacaat 1620
tacagctctg atcacttctg atctttcgag aggcatattg ggcactgctt ctttgattac 1680
agcaacaata acgtcaccaa tatgagcata tcgttgatta ctagctccta tgattcgaat 1740
acacatcaat tctcgagccc cgctgttgtc cgctacattc aaatgggtct gaggttgaat 1800
catatcattt tttttttaac aaagatcaaa gtgaaattca aaaaaaaaaa actgggtggg 1860
ccattcagcg tatgttcgcc ccctcgccca tattcatatt gaaggttagt tagtcatttg 1920
gataaacttt atgccccata tctatgatat ttggattctc acatgatgca ttctgagtcg 1980
aaatgcgaaa taaaagagaa gtctctacct tccctaaagt aaatataaat aaagataggg 2040
tagacaaaat gactaattct atgtggatcc tgaatcctaa aaaacagggg ggttcttggt 2100
attaattcca tcgctggaat taaagctcct gagactgggg tgggacaaaa tcaaacaaaa 2160
tagtaacaac gaattgatat gaacccattt tgctttgtac ttatacaaga caggatagca 2220
tcccataaga agatcctttt aaattggctt tggatatcat tcatgatccc cagggcagaa 2280
ttaagacaga tcaaactaaa gatattaagc ataacaaaca ttttgttctt ggagaaaatt 2340
tcattattat tgggttattg atataagggg aaaatgaaga aagaagggaa agtaggccgc 2400
aaaatctttc tttttctttg aactccctca atcaaaaatc cttcaattct caaatgagaa 2460
tagaaggaat cataccttac atacaatatc ttaattgaat tatatgtaat gatcaataaa 2520
ttcattttga ttctacatct acatgtgtag aatcatagca acaaccaatt caatagatat 2580
tggggctgga attgacgaac aaccaatacc gatattagtg tttatcggtg tcgaatagaa 2640
ataaaaatgg ggcgtggcca agtggtaagg caacgggttt tggtcccgtt actcggaggt 2700
<210> 8
<211> 2698
<212> DNA
<213> Phoebe sheareri
<400> 8
gaagggattg atgaaacaaa tcaaaaaaaa attcacttta tttcgactat aaaaaagtct 60
ctttctaata ttagtaataa taaatcaaag atttctggtg acctatattc cttttcacaa 120
gcatctgtat tttacaaatt atcacaaatc caagctatta agaagtctca tttgagatct 180
ctacttcaat atcgcgaagc atatcttatt cttaaggaaa gaatcaggaa tttttttgga 240
acacgaagaa tatttgattc caaatcaagg cataaaaaac ttcagaattc tggaatgaat 300
gaatggaaaa actggttaag gggtcattat caatacaatt tatctcagga tttatcgata 360
atcttttttg ttgattcaag gaaaagttgt acattgactc tagaaatatt aacagtactt 420
agaaagatat gtatgtatat tctttcgttg aaaaatgtca aaaaatagtg ccatttgcgt 480
atgaatatga atctgttact tcttcttttt gatatctgcc aaatgggttt ctgcgattcc 540
gatcttttat caccacaact tgtatcgtta ggactaatat ttatatccgg agttagaaat 600
atttttcttt tgtcttttgc acccctttct atttgatttc tgattagggt tgttctatcg 660
ttttcctcct cccgctcttc aaaagaatcg atcaatttgt atgatcgtcg aggaatcttt 720
ttaccgattt cttctattcc aatagatttt ttttgaattg tttgattatt tggatcagtt 780
gtaattacat cgaatagaaa tttcaaacat tttgttttat tttctgaatc aaatcttctt 840
tgttcggata ttaaaacaag ctttttaaaa ttaagattaa aaccagttgt tgatttccta 900
gctaattcac tgatagaggt caagaaagga ccaatgtctg tcgataatga ttctccatta 960
aatatatcca ttttatgttc aagtttttgg taatcagtag gaagcatatc atgaatctta 1020
tttatccaaa ccattcctat agaatcttct gtccccattc cacctaaaat cctaggaatt 1080
cgttgatggt tggaatagat tcgtagaccg ggacgactga tacgctttaa aatatttcta 1140
tatgttcctt tcctattcct tctatgtcgc agggttgaaa ccaaaaaata tttgttgttt 1200
tccctatgtt tcctaacatt ttcaataaac ccttcttgta gaagtatttt aacaatgttt 1260
tcggcgatat tagtagatgc tattcgaacc gttccttttt tatccatgtt agcatttctt 1320
atagaagtta ttatatcggc aatagtgtcc ctacccatga cgaactaaaa ttatgggtgc 1380
cttccagttt tgatataatc aacatgttac tttttttttt tcatctacta gtatttataa 1440
gacttcagga gctaatgaga ctattttagt gaaattcaac tgtctcaatt cccgcgcgat 1500
cgctccaaaa actcgagttc cttttggatt tccttcttga tcaatgacaa ctgctgcatt 1560
gtcatcatat cgtattatca taccgttgtc gcgcttgagt tctttacatg tacgtacaat 1620
tacagctctg atcacttctg atctttcgag aggcatattg ggcactgctt ctttgattac 1680
agcaacaata acgtcaccaa tatgagcata tcgttgatta ctagctccta tgattcgaat 1740
acacatcaat tctcgagccc cgctgttgtc cgctacattc aaatgggtct gaggttgaat 1800
catatcattt tttttttaac aaagatcaaa gtgaaattca aaaaaaaaac tgggtgggcc 1860
attcagcgta tgttcgcccc ctcgcccata ttcatattga aggttagtta gtcatttgga 1920
taaactttat gccccatatc tatgatattt ggattctcac atgatgcatt ctgagtcgaa 1980
atgcgaaata aaagagaagt ctctaccttc cctaaagtaa atataaataa agatagggta 2040
gacaaaatga ctaattctat gtggatcctg aatcctaaaa aacagggggg ttcttggtat 2100
taattccatc gctggaatta aagctcctga gactggggtg ggacaaaatc aaacaaaata 2160
gtaacaacga attgatatga acccattttg ctttgtactt atacaagaca ggatagcatc 2220
ccataagaag atccttttaa attggctttg gatatgattc atgatcccca gggcagaatt 2280
aagacagatc aaactaaaga tattaagcat aacaaacatt ttgttcttgg agaaaatttc 2340
attattattg ggttattgat ataaggggaa aatgaagaaa gaagggaaag taggccgcaa 2400
aatctttctt tttctttgaa ctccctcaat caaaaatcct tcaattctca aatgagaata 2460
gaaggaatca taccttacat acaatatctt aattgaatta tatgtaatga tcaataaatt 2520
cattttgatt ctacatctac atgtgtagaa tcatagcaac aaccaattca atagatattg 2580
gggctggaat tgacgaacaa ccaataccga tattagtgtt tatcggtgtc gaatagaaat 2640
aaaaatgggg cgtggccaag tggtaaggca acgggttttg gtcccgttac tcggaggt 2698
<210> 9
<211> 2698
<212> DNA
<213> Phoebe sheareri
<400> 9
gaagggattg atgaaacaaa tcaaaaaaaa attcacttta tttcgactat aaaaaagtct 60
ctttctaata ttagtaataa taaatcaaag atttctggtg acctatattc cttttcacaa 120
gcatctgtat tttacaaatt atcacaaatc caagctatta agaagtctca tttgagatct 180
ctacttcaat atcgcgaagc atatcttatt cttaaggaaa gaatcaggaa tttttttgga 240
acacgaagaa tatttgattc caaatcaagg cataaaaaac ttcagaattc tggaatgaat 300
gaatggaaaa actggttaag gggtcattat caatacaatt tatctcagga tttatcgata 360
atcttttttg ttgattcaag gaaaagttgt acattgactc tagaaatatt aacagtactt 420
agaaagatat gtatgtatat tctttcgttg aaaaatgtca aaaaatagtg ccatttgcgt 480
atgaatatga atctgttact tcttcttttt gatatctgcc aaatgggttt ctgcgattcc 540
gatcttttat caccacaact tgtatcgtta ggactaatat ttatatccgg agttagaaat 600
atttttcttt tgtcttttgc acccctttct atttgatttc tgattagggt tgttctatcg 660
ttttcctcct cccgctcttc aaaagaatcg atcaatttgt atgatcgtcg aggaatcttt 720
ttaccgattt cttctattcc aatagatttt ttttgaattg tttgattatt tggatcagtt 780
gtaattacat cgaatagaaa tttcaaacat tttgttttat tttctgaatc aaatcttctt 840
tgttcggata ttaaaacaag ctttttaaaa ttaagattaa aaccagttgt tgatttccta 900
gctaattcac tgatagaggt caagaaagga ccaatgtctg tcgataatga ttctccatta 960
aatatatcca ttttatgttc aagtttttgg taatcagtag gaagcatatc atgaatctta 1020
tttatccaaa ccattcctat agaatcttct gtccccattc cacctaaaat cctaggaatt 1080
cgttgatggt tggaatagat tcgtagaccg ggacgactga tacgctttaa aatatttcta 1140
tatgttcctt tcctattcct tctatgtcgc agggttgaaa ccaaaaaata tttgttgttt 1200
tccctatgtt tcctaacatt ttcaataaac ccttcttgta gaagtatttt aacaatgttt 1260
tcggcgatat tagtagatgc tattcgaacc gttccttttt tatccatgtt agcatttctt 1320
atagaagtta ttatatcggc aatagtgtcc ctacccatga cgaactaaaa ttatgggtgc 1380
cttccagttt tgatataatc aacatgttac tttttttttt tcatctacta gtatttataa 1440
gacttcagga gctaatgaga ctattttagt gaaattcaac tgtctcaatt cccgcgcgat 1500
cgctccaaaa actcgagttc cttttggatt tccttcttga tcaatgacaa ctgctgcatt 1560
gtcatcatat cgtattatca taccgttgtc gcgcttgagt tctttacatg tacgtacaat 1620
tacagctctg atcacttctg atctttcgag aggcatattg ggcactgctt ctttgattac 1680
agcaacaata acgtcaccaa tatgagcata tcgttgatta ctagctccta tgattcgaat 1740
acacatcaat tctcgagccc cgctgttgtc cgctacattc aaatgggtct gaggttgaat 1800
catatcattt tttttttaac aaagatcaaa gtgaaattca aaaaaaaaac tgggtgggcc 1860
attcagcgta tgttcgcccc ctcgcccata ttcatattga aggttagtta gtcatttgga 1920
taaactttat gccccatatc tatgatattt ggattctcac atgatgcatt ctgagtcgaa 1980
atgcgaaata aaagagaagt ctctaccttc cctaaagtaa atataaataa agatagggta 2040
gacaaaatga ctaattctat gtggatcctg aatcctaaaa aacagggggg ttcttggtat 2100
taattccatc gctggaatta aagctcctga gactggggtg ggacaaaatc aaacaaaata 2160
gtaacaacga attgatatga acccattttg ctttgtactt atacaagaca ggatagcatc 2220
ccataagaag atccttttaa attggctttg gatatgattc atgatcccca gggcagaatt 2280
aagacagatc aaactaaaga tattaagcat aacaaacatt ttgttcttgg agaaaatttc 2340
attattattg ggttattgat ataaggggaa aatgaagaaa gaagggaaag taggccgcaa 2400
aatctttctt tttctttgaa ctccctcaat caaaaatcct tcaattctca aatgagaata 2460
gaaggaatca taccttacat acaatatctt aattgaatta tatgtaatga tcaataaatt 2520
cattttgatt ctacatctac atgtgtagaa tcatagcaac aaccaattca atagatattg 2580
gggctggaat tgacgaacaa ccaataccga tattagtgtt tatcggtgtc gaatagaaat 2640
aaaaatgggg cgtggccaag tggtaaggca acgggttttg gtcccgttac tcggaggt 2698
<210> 10
<211> 2698
<212> DNA
<213> Phoebe sheareri
<400> 10
gaagggattg atgaaacaaa tcaaaaaaaa attcacttta tttcgactat aaaaaagtct 60
ctttctaata ttagtaataa taaatcaaag atttctggtg acctatattc cttttcacaa 120
gcatctgtat tttacaaatt atcacaaatc caagctatta agaagtctca tttgagatct 180
ctacttcaat atcgcgaagc atatcttatt cttaaggaaa gaatcaggaa tttttttgga 240
acacgaagaa tatttgattc caaatcaagg cataaaaaac ttcagaattc tggaatgaat 300
gaatggaaaa actggttaag gggtcattat caatacaatt tatctcagga tttatcgata 360
atcttttttg ttgattcaag gaaaagttgt acattgactc tagaaatatt aacagtactt 420
agaaagatat gtatgtatat tctttcgttg aaaaatgtca aaaaatagtg ccatttgcgt 480
atgaatatga atctgttact tcttcttttt gatatctgcc aaatgggttt ctgcgattcc 540
gatcttttat caccacaact tgtatcgtta ggactaatat ttatatccgg agttagaaat 600
atttttcttt tgtcttttgc acccctttct atttgatttc tgattagggt tgttctatcg 660
ttttcctcct cccgctcttc aaaagaatcg atcaatttgt atgatcgtcg aggaatcttt 720
ttaccgattt cttctattcc aatagatttt ttttgaattg tttgattatt tggatcagtt 780
gtaattacat cgaatagaaa tttcaaacat tttgttttat tttctgaatc aaatcttctt 840
tgttcggata ttaaaacaag ctttttaaaa ttaagattaa aaccagttgt tgatttccta 900
gctaattcac tgatagaggt caagaaagga ccaatgtctg tcgataatga ttctccatta 960
aatatatcca ttttatgttc aagtttttgg taatcagtag gaagcatatc atgaatctta 1020
tttatccaaa ccattcctat agaatcttct gtccccattc cacctaaaat cctaggaatt 1080
cgttgatggt tggaatagat tcgtagaccg ggacgactga tacgctttaa aatatttcta 1140
tatgttcctt tcctattcct tctatgtcgc agggttgaaa ccaaaaaata tttgttgttt 1200
tccctatgtt tcctaacatt ttcaataaac ccttcttgta gaagtatttt aacaatgttt 1260
tcggcgatat tagtagatgc tattcgaacc gttccttttt tatccatgtt agcatttctt 1320
atagaagtta ttatatcggc aatagtgtcc ctacccatga cgaactaaaa ttatgggtgc 1380
cttccagttt tgatataatc aacatgttac tttttttttt tcatctacta gtatttataa 1440
gacttcagga gctaatgaga ctattttagt gaaattcaac tgtctcaatt cccgcgcgat 1500
cgctccaaaa actcgagttc cttttggatt tccttcttga tcaatgacaa ctgctgcatt 1560
gtcatcatat cgtattatca taccgttgtc gcgcttgagt tctttacatg tacgtacaat 1620
tacagctctg atcacttctg atctttcgag aggcatattg ggcactgctt ctttgattac 1680
agcaacaata acgtcaccaa tatgagcata tcgttgatta ctagctccta tgattcgaat 1740
acacatcaat tctcgagccc cgctgttgtc cgctacattc aaatgggtct gaggttgaat 1800
catatcattt tttttttaac aaagatcaaa gtgaaattca aaaaaaaaac tgggtgggcc 1860
attcagcgta tgttcgcccc ctcgcccata ttcatattga aggttagtta gtcatttgga 1920
taaactttat gccccatatc tatgatattt ggattctcac atgatgcatt ctgagtcgaa 1980
atgcgaaata aaagagaagt ctctaccttc cctaaagtaa atataaataa agatagggta 2040
gacaaaatga ctaattctat gtggatcctg aatcctaaaa aacagggggg ttcttggtat 2100
taattccatc gctggaatta aagctcctga gactggggtg ggacaaaatc aaacaaaata 2160
gtaacaacga attgatatga acccattttg ctttgtactt atacaagaca ggatagcatc 2220
ccataagaag atccttttaa attggctttg gatatgattc atgatcccca gggcagaatt 2280
aagacagatc aaactaaaga tattaagcat aacaaacatt ttgttcttgg agaaaatttc 2340
attattattg ggttattgat ataaggggaa aatgaagaaa gaagggaaag taggccgcaa 2400
aatctttctt tttctttgaa ctccctcaat caaaaatcct tcaattctca aatgagaata 2460
gaaggaatca taccttacat acaatatctt aattgaatta tatgtaatga tcaataaatt 2520
cattttgatt ctacatctac atgtgtagaa tcatagcaac aaccaattca atagatattg 2580
gggctggaat tgacgaacaa ccaataccga tattagtgtt tatcggtgtc gaatagaaat 2640
aaaaatgggg cgtggccaag tggtaaggca acgggttttg gtcccgttac tcggaggt 2698
<210> 11
<211> 2698
<212> DNA
<213> Phoebe sheareri
<400> 11
gaagggattg atgaaacaaa tcaaaaaaaa attcacttta tttcgactat aaaaaagtct 60
ctttctaata ttagtaataa taaatcaaag atttctggtg acctatattc cttttcacaa 120
gcatctgtat tttacaaatt atcacaaatc caagctatta agaagtctca tttgagatct 180
ctacttcaat atcgcgaagc atatcttatt cttaaggaaa gaatcaggaa tttttttgga 240
acacgaagaa tatttgattc caaatcaagg cataaaaaac ttcagaattc tggaatgaat 300
gaatggaaaa actggttaag gggtcattat caatacaatt tatctcagga tttatcgata 360
atcttttttg ttgattcaag gaaaagttgt acattgactc tagaaatatt aacagtactt 420
agaaagatat gtatgtatat tctttcgttg aaaaatgtca aaaaatagtg ccatttgcgt 480
atgaatatga atctgttact tcttcttttt gatatctgcc aaatgggttt ctgcgattcc 540
gatcttttat caccacaact tgtatcgtta ggactaatat ttatatccgg agttagaaat 600
atttttcttt tgtcttttgc acccctttct atttgatttc tgattagggt tgttctatcg 660
ttttcctcct cccgctcttc aaaagaatcg atcaatttgt atgatcgtcg aggaatcttt 720
ttaccgattt cttctattcc aatagatttt ttttgaattg tttgattatt tggatcagtt 780
gtaattacat cgaatagaaa tttcaaacat tttgttttat tttctgaatc aaatcttctt 840
tgttcggata ttaaaacaag ctttttaaaa ttaagattaa aaccagttgt tgatttccta 900
gctaattcac tgatagaggt caagaaagga ccaatgtctg tcgataatga ttctccatta 960
aatatatcca ttttatgttc aagtttttgg taatcagtag gaagcatatc atgaatctta 1020
tttatccaaa ccattcctat agaatcttct gtccccattc cacctaaaat cctaggaatt 1080
cgttgatggt tggaatagat tcgtagaccg ggacgactga tacgctttaa aatatttcta 1140
tatgttcctt tcctattcct tctatgtcgc agggttgaaa ccaaaaaata tttgttgttt 1200
tccctatgtt tcctaacatt ttcaataaac ccttcttgta gaagtatttt aacaatgttt 1260
tcggcgatat tagtagatgc tattcgaacc gttccttttt tatccatgtt agcatttctt 1320
atagaagtta ttatatcggc aatagtgtcc ctacccatga cgaactaaaa ttatgggtgc 1380
cttccagttt tgatataatc aacatgttac tttttttttt tcatctacta gtatttataa 1440
gacttcagga gctaatgaga ctattttagt gaaattcaac tgtctcaatt cccgcgcgat 1500
cgctccaaaa actcgagttc cttttggatt tccttcttga tcaatgacaa ctgctgcatt 1560
gtcatcatat cgtattatca taccgttgtc gcgcttgagt tctttacatg tacgtacaat 1620
tacagctctg atcacttctg atctttcgag aggcatattg ggcactgctt ctttgattac 1680
agcaacaata acgtcaccaa tatgagcata tcgttgatta ctagctccta tgattcgaat 1740
acacatcaat tctcgagccc cgctgttgtc cgctacattc aaatgggtct gaggttgaat 1800
catatcattt tttttttaac aaagatcaaa gtgaaattca aaaaaaaaac tgggtgggcc 1860
attcagcgta tgttcgcccc ctcgcccata ttcatattga aggttagtta gtcatttgga 1920
taaactttat gccccatatc tatgatattt ggattctcac atgatgcatt ctgagtcgaa 1980
atgcgaaata aaagagaagt ctctaccttc cctaaagtaa atataaataa agatagggta 2040
gacaaaatga ctaattctat gtggatcctg aatcctaaaa aacagggggg ttcttggtat 2100
taattccatc gctggaatta aagctcctga gactggggtg ggacaaaatc aaacaaaata 2160
gtaacaacga attgatatga acccattttg ctttgtactt atacaagaca ggatagcatc 2220
ccataagaag atccttttaa attggctttg gatatgattc atgatcccca gggcagaatt 2280
aagacagatc aaactaaaga tattaagcat aacaaacatt ttgttcttgg agaaaatttc 2340
attattattg ggttattgat ataaggggaa aatgaagaaa gaagggaaag taggccgcaa 2400
aatctttctt tttctttgaa ctccctcaat caaaaatcct tcaattctca aatgagaata 2460
gaaggaatca taccttacat acaatatctt aattgaatta tatgtaatga tcaataaatt 2520
cattttgatt ctacatctac atgtgtagaa tcatagcaac aaccaattca atagatattg 2580
gggctggaat tgacgaacaa ccaataccga tattagtgtt tatcggtgtc gaatagaaat 2640
aaaaatgggg cgtggccaag tggtaaggca acgggttttg gtcccgttac tcggaggt 2698
<210> 12
<211> 2698
<212> DNA
<213> Phoebe sheareri
<400> 12
gaagggattg atgaaacaaa tcaaaaaaaa attcacttta tttcgactat aaaaaagtct 60
ctttctaata ttagtaataa taaatcaaag atttctggtg acctatattc cttttcacaa 120
gcatctgtat tttacaaatt atcacaaatc caagctatta agaagtctca tttgagatct 180
ctacttcaat atcgcgaagc atatcttatt cttaaggaaa gaatcaggaa tttttttgga 240
acacgaagaa tatttgattc caaatcaagg cataaaaaac ttcagaattc tggaatgaat 300
gaatggaaaa actggttaag gggtcattat caatacaatt tatctcagga tttatcgata 360
atcttttttg ttgattcaag gaaaagttgt acattgactc tagaaatatt aacagtactt 420
agaaagatat gtatgtatat tctttcgttg aaaaatgtca aaaaatagtg ccatttgcgt 480
atgaatatga atctgttact tcttcttttt gatatctgcc aaatgggttt ctgcgattcc 540
gatcttttat caccacaact tgtatcgtta ggactaatat ttatatccgg agttagaaat 600
atttttcttt tgtcttttgc acccctttct atttgatttc tgattagggt tgttctatcg 660
ttttcctcct cccgctcttc aaaagaatcg atcaatttgt atgatcgtcg aggaatcttt 720
ttaccgattt cttctattcc aatagatttt ttttgaattg tttgattatt tggatcagtt 780
gtaattacat cgaatagaaa tttcaaacat tttgttttat tttctgaatc aaatcttctt 840
tgttcggata ttaaaacaag ctttttaaaa ttaagattaa aaccagttgt tgatttccta 900
gctaattcac tgatagaggt caagaaagga ccaatgtctg tcgataatga ttctccatta 960
aatatatcca ttttatgttc aagtttttgg taatcagtag gaagcatatc atgaatctta 1020
tttatccaaa ccattcctat agaatcttct gtccccattc cacctaaaat cctaggaatt 1080
cgttgatggt tggaatagat tcgtagaccg ggacgactga tacgctttaa aatatttcta 1140
tatgttcctt tcctattcct tctatgtcgc agggttgaaa ccaaaaaata tttgttgttt 1200
tccctatgtt tcctaacatt ttcaataaac ccttcttgta gaagtatttt aacaatgttt 1260
tcggcgatat tagtagatgc tattcgaacc gttccttttt tatccatgtt agcatttctt 1320
atagaagtta ttatatcggc aatagtgtcc ctacccatga cgaactaaaa ttatgggtgc 1380
cttccagttt tgatataatc aacatgttac tttttttttt tcatctacta gtatttataa 1440
gacttcagga gctaatgaga ctattttagt gaaattcaac tgtctcaatt cccgcgcgat 1500
cgctccaaaa actcgagttc cttttggatt tccttcttga tcaatgacaa ctgctgcatt 1560
gtcatcatat cgtattatca taccgttgtc gcgcttgagt tctttacatg tacgtacaat 1620
tacagctctg atcacttctg atctttcgag aggcatattg ggcactgctt ctttgattac 1680
agcaacaata acgtcaccaa tatgagcata tcgttgatta ctagctccta tgattcgaat 1740
acacatcaat tctcgagccc cgctgttgtc cgctacattc aaatgggtct gaggttgaat 1800
catatcattt tttttttaac aaagatcaaa gtgaaattca aaaaaaaaac tgggtgggcc 1860
attcagcgta tgttcgcccc ctcgcccata ttcatattga aggttagtta gtcatttgga 1920
taaactttat gccccatatc tatgatattt ggattctcac atgatgcatt ctgagtcgaa 1980
atgcgaaata aaagagaagt ctctaccttc cctaaagtaa atataaataa agatagggta 2040
gacaaaatga ctaattctat gtggatcctg aatcctaaaa aacagggggg ttcttggtat 2100
taattccatc gctggaatta aagctcctga gactggggtg ggacaaaatc aaacaaaata 2160
gtaacaacga attgatatga acccattttg ctttgtactt atacaagaca ggatagcatc 2220
ccataagaag atccttttaa attggctttg gatatgattc atgatcccca gggcagaatt 2280
aagacagatc aaactaaaga tattaagcat aacaaacatt ttgttcttgg agaaaatttc 2340
attattattg ggttattgat ataaggggaa aatgaagaaa gaagggaaag taggccgcaa 2400
aatctttctt tttctttgaa ctccctcaat caaaaatcct tcaattctca aatgagaata 2460
gaaggaatca taccttacat acaatatctt aattgaatta tatgtaatga tcaataaatt 2520
cattttgatt ctacatctac atgtgtagaa tcatagcaac aaccaattca atagatattg 2580
gggctggaat tgacgaacaa ccaataccga tattagtgtt tatcggtgtc gaatagaaat 2640
aaaaatgggg cgtggccaag tggtaaggca acgggttttg gtcccgttac tcggaggt 2698
<210> 13
<211> 2699
<212> DNA
<213> Phoebe chekiangensis
<400> 13
gaagggattg atgaaacaaa tcaaaaaaaa attcacttta tttcgactat aaaaaagtct 60
ctttctaata ttagtaataa taaatcaaag atttctggtg acctatattc cttttcacaa 120
gcatctgtat tttacaaatt atcacaaatc caagctatta agaagtatca tttgagatct 180
ctacttcaat atcgcgaagc atatcttatt cttaaggaaa gaatcaggaa tttttttgga 240
acacgaagaa tatttgattc caaatcaagg cataaaaaac ttcagaattc tggaatgaat 300
gaatggaaaa actggttaag gggtcattat caatacaatt tatctcagga tttatcgata 360
atcttttttg ttgattcaag gaaaagttgt acattgactc tagaaatatt aacagtactt 420
agaaagatat gtatgtatat tctttcgttg aaaaatatca aaaaatagtg ccatttgcgt 480
atgaatatga atcttttact tcttcttttt gatatctgcc aaatgggttt ctgcgattcc 540
gatcttttat caccacaact tgtatcgtta ggactaatat ttatatccgg agttagaaat 600
atttttcttt tgtcttttgc acccctttct atttgatttc tgattagggt tgttctatcg 660
ttttcctcct cccgctcttc aaaagaatcg atcaatttgt atgatcgtcg aggaatcttt 720
ttaccgattt cttctattcc aatagatttt ttttgaattg tttgattatt tggatcagtt 780
gtaattacat cgaatagaaa tttcaaacat tttgttttat tttctgaatc aaatcttctt 840
tgttcggata ttaaaacaag ctttttaaaa ttaagattaa aaccagttgt tgatttccta 900
gctaattcac tgatagaggt caagaaagga ccaatgtctg tcgataatga ttctccatta 960
aatatatcca ttttatgttc aagtttttgg taatcagtag gaagcatatc atgaatctta 1020
tttatccaaa ccattcctat agaatcttct gtccccattc cacctaaaat cctaggaatt 1080
cgttgatggt tggaatagat tcgtagaccg ggacgactga tacgctttaa aatatttcta 1140
tatgttcctt tcctattcct tctatgtcgc agggttgaaa ccaaaaaata tttgttgttt 1200
tccctatgtt tcctaacatt ttcaataaac ccttcttgta gaagtatttt aacaatgttt 1260
tcggcgatat tagtagatgc tattcgaacc gttccttttt tatccatgtt agcatttctt 1320
atagaagtta ttatatcggc aatagtgtcc ctacccatga cgaactaaaa ttatgggtgc 1380
cttccagttt tgatataatc aacatgttac tttttttttt tcatctacta gtatttataa 1440
gacttcagga gctaatgaga ctattttagt gaaattcaac tgtctcaatt cccgcgcgat 1500
cgctccaaaa actcgagttc cttttggatt tccttcttga tcaatgacaa ctgctgcatt 1560
gtcatcatat cgtattatca taccgttgtc gcgcttgagt tctttacatg tacgtacaat 1620
tacagctctg atcacttctg atctttcgag aggcatattg ggcactgctt ctttgattac 1680
agcaacaata acgtcaccaa tatgagcata tcgttgatta ctagctccta tgattcgaat 1740
acacatcaat tctcgagccc cgctgttgtc cgctacattc aaatgggtct gaggttgaat 1800
catatcattt tttttttaac aaagatcaaa gtgaaattca aaaaaaaaaa ctgggtgggc 1860
cattcagcgt atgttcgccc cctcgcccat attcatattg aaggttagtc agtcatttgg 1920
ataaacttta tgccccatat ctatgatatt tggattctca catgatgcat tctgagtcga 1980
aatgcgaaat aaaagagaag tctctacctt ccctaaagta aatataaata aagatagggt 2040
agacaaaatg actaattcta tgtggatcct gaatcctaaa aaacaggggg gttcttggta 2100
ttaattccat cgctggaatt aaagctcctg agactggggt gggacaaaat caaacaaaat 2160
agtaacaacg aattgatatg aacccatttt gctttgtact tatacaagac aggatagcat 2220
cccataagaa gatcctttta aattggcttt ggatatgatt catgatcccc agggcagaat 2280
taagacagat caaactaaag atattaagca taacaaacat tttgttcttg gagaaaattt 2340
cattattatt gggttattga tataagggga aaatgaagaa agaagggaaa gtaggccgca 2400
aaatctttct ttttctttga actccctcaa tcaaaaatcc ttcaattctc aaatgagaat 2460
agaaggaatc ataccttaca tacaatatct taattgaatt atatgtaatg atcaataaat 2520
tcattttgat tctacatcta catgtgtaga atcatagcaa caaccaattc aatagatatt 2580
ggggctggaa ttgacgaaca accaataccg atattagtgt ttatcagtgt cgaatagaaa 2640
taaaaatggg gcgtggccaa gtggtaaggc aacgggtttt ggtcccgtta ctcggaggt 2699
<210> 14
<211> 2699
<212> DNA
<213> Phoebe chekiangensis
<400> 14
gaagggattg atgaaacaaa tcaaaaaaaa attcacttta tttcgactat aaaaaagtct 60
ctttctaata ttagtaataa taaatcaaag atttctggtg acctatattc cttttcacaa 120
gcatctgtat tttacaaatt atcacaaatc caagctatta agaagtatca tttgagatct 180
ctacttcaat atcgcgaagc atatcttatt cttaaggaaa gaatcaggaa tttttttgga 240
acacgaagaa tatttgattc caaatcaagg cataaaaaac ttcagaattc tggaatgaat 300
gaatggaaaa actggttaag gggtcattat caatacaatt tatctcagga tttatcgata 360
atcttttttg ttgattcaag gaaaagttgt acattgactc tagaaatatt aacagtactt 420
agaaagatat gtatgtatat tctttcgttg aaaaatatca aaaaatagtg ccatttgcgt 480
atgaatatga atcttttact tcttcttttt gatatctgcc aaatgggttt ctgcgattcc 540
gatcttttat caccacaact tgtatcgtta ggactaatat ttatatccgg agttagaaat 600
atttttcttt tgtcttttgc acccctttct atttgatttc tgattagggt tgttctatcg 660
ttttcctcct cccgctcttc aaaagaatcg atcaatttgt atgatcgtcg aggaatcttt 720
ttaccgattt cttctattcc aatagatttt ttttgaattg tttgattatt tggatcagtt 780
gtaattacat cgaatagaaa tttcaaacat tttgttttat tttctgaatc aaatcttctt 840
tgttcggata ttaaaacaag ctttttaaaa ttaagattaa aaccagttgt tgatttccta 900
gctaattcac tgatagaggt caagaaagga ccaatgtctg tcgataatga ttctccatta 960
aatatatcca ttttatgttc aagtttttgg taatcagtag gaagcatatc atgaatctta 1020
tttatccaaa ccattcctat agaatcttct gtccccattc cacctaaaat cctaggaatt 1080
cgttgatggt tggaatagat tcgtagaccg ggacgactga tacgctttaa aatatttcta 1140
tatgttcctt tcctattcct tctatgtcgc agggttgaaa ccaaaaaata tttgttgttt 1200
tccctatgtt tcctaacatt ttcaataaac ccttcttgta gaagtatttt aacaatgttt 1260
tcggcgatat tagtagatgc tattcgaacc gttccttttt tatccatgtt agcatttctt 1320
atagaagtta ttatatcggc aatagtgtcc ctacccatga cgaactaaaa ttatgggtgc 1380
cttccagttt tgatataatc aacatgttac tttttttttt tcatctacta gtatttataa 1440
gacttcagga gctaatgaga ctattttagt gaaattcaac tgtctcaatt cccgcgcgat 1500
cgctccaaaa actcgagttc cttttggatt tccttcttga tcaatgacaa ctgctgcatt 1560
gtcatcatat cgtattatca taccgttgtc gcgcttgagt tctttacatg tacgtacaat 1620
tacagctctg atcacttctg atctttcgag aggcatattg ggcactgctt ctttgattac 1680
agcaacaata acgtcaccaa tatgagcata tcgttgatta ctagctccta tgattcgaat 1740
acacatcaat tctcgagccc cgctgttgtc cgctacattc aaatgggtct gaggttgaat 1800
catatcattt tttttttaac aaagatcaaa gtgaaattca aaaaaaaaaa ctgggtgggc 1860
cattcagcgt atgttcgccc cctcgcccat attcatattg aaggttagtc agtcatttgg 1920
ataaacttta tgccccatat ctatgatatt tggattctca catgatgcat tctgagtcga 1980
aatgcgaaat aaaagagaag tctctacctt ccctaaagta aatataaata aagatagggt 2040
agacaaaatg actaattcta tgtggatcct gaatcctaaa aaacaggggg gttcttggta 2100
ttaattccat cgctggaatt aaagctcctg agactggggt gggacaaaat caaacaaaat 2160
agtaacaacg aattgatatg aacccatttt gctttgtact tatacaagac aggatagcat 2220
cccataagaa gatcctttta aattggcttt ggatatgatt catgatcccc agggcagaat 2280
taagacagat caaactaaag atattaagca taacaaacat tttgttcttg gagaaaattt 2340
cattattatt gggttattga tataagggga aaatgaagaa agaagggaaa gtaggccgca 2400
aaatctttct ttttctttga actccctcaa tcaaaaatcc ttcaattctc aaatgagaat 2460
agaaggaatc ataccttaca tacaatatct taattgaatt atatgtaatg atcaataaat 2520
tcattttgat tctacatcta catgtgtaga atcatagcaa caaccaattc aatagatatt 2580
ggggctggaa ttgacgaaca accaataccg atattagtgt ttatcagtgt cgaatagaaa 2640
taaaaatggg gcgtggccaa gtggtaaggc aacgggtttt ggtcccgtta ctcggaggt 2699
<210> 15
<211> 2699
<212> DNA
<213> Phoebe chekiangensis
<400> 15
gaagggattg atgaaacaaa tcaaaaaaaa attcacttta tttcgactat aaaaaagtct 60
ctttctaata ttagtaataa taaatcaaag atttctggtg acctatattc cttttcacaa 120
gcatctgtat tttacaaatt atcacaaatc caagctatta agaagtatca tttgagatct 180
ctacttcaat atcgcgaagc atatcttatt cttaaggaaa gaatcaggaa tttttttgga 240
acacgaagaa tatttgattc caaatcaagg cataaaaaac ttcagaattc tggaatgaat 300
gaatggaaaa actggttaag gggtcattat caatacaatt tatctcagga tttatcgata 360
atcttttttg ttgattcaag gaaaagttgt acattgactc tagaaatatt aacagtactt 420
agaaagatat gtatgtatat tctttcgttg aaaaatatca aaaaatagtg ccatttgcgt 480
atgaatatga atcttttact tcttcttttt gatatctgcc aaatgggttt ctgcgattcc 540
gatcttttat caccacaact tgtatcgtta ggactaatat ttatatccgg agttagaaat 600
atttttcttt tgtcttttgc acccctttct atttgatttc tgattagggt tgttctatcg 660
ttttcctcct cccgctcttc aaaagaatcg atcaatttgt atgatcgtcg aggaatcttt 720
ttaccgattt cttctattcc aatagatttt ttttgaattg tttgattatt tggatcagtt 780
gtaattacat cgaatagaaa tttcaaacat tttgttttat tttctgaatc aaatcttctt 840
tgttcggata ttaaaacaag ctttttaaaa ttaagattaa aaccagttgt tgatttccta 900
gctaattcac tgatagaggt caagaaagga ccaatgtctg tcgataatga ttctccatta 960
aatatatcca ttttatgttc aagtttttgg taatcagtag gaagcatatc atgaatctta 1020
tttatccaaa ccattcctat agaatcttct gtccccattc cacctaaaat cctaggaatt 1080
cgttgatggt tggaatagat tcgtagaccg ggacgactga tacgctttaa aatatttcta 1140
tatgttcctt tcctattcct tctatgtcgc agggttgaaa ccaaaaaata tttgttgttt 1200
tccctatgtt tcctaacatt ttcaataaac ccttcttgta gaagtatttt aacaatgttt 1260
tcggcgatat tagtagatgc tattcgaacc gttccttttt tatccatgtt agcatttctt 1320
atagaagtta ttatatcggc aatagtgtcc ctacccatga cgaactaaaa ttatgggtgc 1380
cttccagttt tgatataatc aacatgttac tttttttttt tcatctacta gtatttataa 1440
gacttcagga gctaatgaga ctattttagt gaaattcaac tgtctcaatt cccgcgcgat 1500
cgctccaaaa actcgagttc cttttggatt tccttcttga tcaatgacaa ctgctgcatt 1560
gtcatcatat cgtattatca taccgttgtc gcgcttgagt tctttacatg tacgtacaat 1620
tacagctctg atcacttctg atctttcgag aggcatattg ggcactgctt ctttgattac 1680
agcaacaata acgtcaccaa tatgagcata tcgttgatta ctagctccta tgattcgaat 1740
acacatcaat tctcgagccc cgctgttgtc cgctacattc aaatgggtct gaggttgaat 1800
catatcattt tttttttaac aaagatcaaa gtgaaattca aaaaaaaaaa ctgggtgggc 1860
cattcagcgt atgttcgccc cctcgcccat attcatattg aaggttagtc agtcatttgg 1920
ataaacttta tgccccatat ctatgatatt tggattctca catgatgcat tctgagtcga 1980
aatgcgaaat aaaagagaag tctctacctt ccctaaagta aatataaata aagatagggt 2040
agacaaaatg actaattcta tgtggatcct gaatcctaaa aaacaggggg gttcttggta 2100
ttaattccat cgctggaatt aaagctcctg agactggggt gggacaaaat caaacaaaat 2160
agtaacaacg aattgatatg aacccatttt gctttgtact tatacaagac aggatagcat 2220
cccataagaa gatcctttta aattggcttt ggatatgatt catgatcccc agggcagaat 2280
taagacagat caaactaaag atattaagca taacaaacat tttgttcttg gagaaaattt 2340
cattattatt gggttattga tataagggga aaatgaagaa agaagggaaa gtaggccgca 2400
aaatctttct ttttctttga actccctcaa tcaaaaatcc ttcaattctc aaatgagaat 2460
agaaggaatc ataccttaca tacaatatct taattgaatt atatgtaatg atcaataaat 2520
tcattttgat tctacatcta catgtgtaga atcatagcaa caaccaattc aatagatatt 2580
ggggctggaa ttgacgaaca accaataccg atattagtgt ttatcagtgt cgaatagaaa 2640
taaaaatggg gcgtggccaa gtggtaaggc aacgggtttt ggtcccgtta ctcggaggt 2699
<210> 16
<211> 2699
<212> DNA
<213> Phoebe chekiangensis
<400> 16
gaagggattg atgaaacaaa tcaaaaaaaa attcacttta tttcgactat aaaaaagtct 60
ctttctaata ttagtaataa taaatcaaag atttctggtg acctatattc cttttcacaa 120
gcatctgtat tttacaaatt atcacaaatc caagctatta agaagtatca tttgagatct 180
ctacttcaat atcgcgaagc atatcttatt cttaaggaaa gaatcaggaa tttttttgga 240
acacgaagaa tatttgattc caaatcaagg cataaaaaac ttcagaattc tggaatgaat 300
gaatggaaaa actggttaag gggtcattat caatacaatt tatctcagga tttatcgata 360
atcttttttg ttgattcaag gaaaagttgt acattgactc tagaaatatt aacagtactt 420
agaaagatat gtatgtatat tctttcgttg aaaaatatca aaaaatagtg ccatttgcgt 480
atgaatatga atcttttact tcttcttttt gatatctgcc aaatgggttt ctgcgattcc 540
gatcttttat caccacaact tgtatcgtta ggactaatat ttatatccgg agttagaaat 600
atttttcttt tgtcttttgc acccctttct atttgatttc tgattagggt tgttctatcg 660
ttttcctcct cccgctcttc aaaagaatcg atcaatttgt atgatcgtcg aggaatcttt 720
ttaccgattt cttctattcc aatagatttt ttttgaattg tttgattatt tggatcagtt 780
gtaattacat cgaatagaaa tttcaaacat tttgttttat tttctgaatc aaatcttctt 840
tgttcggata ttaaaacaag ctttttaaaa ttaagattaa aaccagttgt tgatttccta 900
gctaattcac tgatagaggt caagaaagga ccaatgtctg tcgataatga ttctccatta 960
aatatatcca ttttatgttc aagtttttgg taatcagtag gaagcatatc atgaatctta 1020
tttatccaaa ccattcctat agaatcttct gtccccattc cacctaaaat cctaggaatt 1080
cgttgatggt tggaatagat tcgtagaccg ggacgactga tacgctttaa aatatttcta 1140
tatgttcctt tcctattcct tctatgtcgc agggttgaaa ccaaaaaata tttgttgttt 1200
tccctatgtt tcctaacatt ttcaataaac ccttcttgta gaagtatttt aacaatgttt 1260
tcggcgatat tagtagatgc tattcgaacc gttccttttt tatccatgtt agcatttctt 1320
atagaagtta ttatatcggc aatagtgtcc ctacccatga cgaactaaaa ttatgggtgc 1380
cttccagttt tgatataatc aacatgttac tttttttttt tcatctacta gtatttataa 1440
gacttcagga gctaatgaga ctattttagt gaaattcaac tgtctcaatt cccgcgcgat 1500
cgctccaaaa actcgagttc cttttggatt tccttcttga tcaatgacaa ctgctgcatt 1560
gtcatcatat cgtattatca taccgttgtc gcgcttgagt tctttacatg tacgtacaat 1620
tacagctctg atcacttctg atctttcgag aggcatattg ggcactgctt ctttgattac 1680
agcaacaata acgtcaccaa tatgagcata tcgttgatta ctagctccta tgattcgaat 1740
acacatcaat tctcgagccc cgctgttgtc cgctacattc aaatgggtct gaggttgaat 1800
catatcattt tttttttaac aaagatcaaa gtgaaattca aaaaaaaaaa ctgggtgggc 1860
cattcagcgt atgttcgccc cctcgcccat attcatattg aaggttagtc agtcatttgg 1920
ataaacttta tgccccatat ctatgatatt tggattctca catgatgcat tctgagtcga 1980
aatgcgaaat aaaagagaag tctctacctt ccctaaagta aatataaata aagatagggt 2040
agacaaaatg actaattcta tgtggatcct gaatcctaaa aaacaggggg gttcttggta 2100
ttaattccat cgctggaatt aaagctcctg agactggggt gggacaaaat caaacaaaat 2160
agtaacaacg aattgatatg aacccatttt gctttgtact tatacaagac aggatagcat 2220
cccataagaa gatcctttta aattggcttt ggatatgatt catgatcccc agggcagaat 2280
taagacagat caaactaaag atattaagca taacaaacat tttgttcttg gagaaaattt 2340
cattattatt gggttattga tataagggga aaatgaagaa agaagggaaa gtaggccgca 2400
aaatctttct ttttctttga actccctcaa tcaaaaatcc ttcaattctc aaatgagaat 2460
agaaggaatc ataccttaca tacaatatct taattgaatt atatgtaatg atcaataaat 2520
tcattttgat tctacatcta catgtgtaga atcatagcaa caaccaattc aatagatatt 2580
ggggctggaa ttgacgaaca accaataccg atattagtgt ttatcagtgt cgaatagaaa 2640
taaaaatggg gcgtggccaa gtggtaaggc aacgggtttt ggtcccgtta ctcggaggt 2699
<210> 17
<211> 2699
<212> DNA
<213> Phoebe chekiangensis
<400> 17
gaagggattg atgaaacaaa tcaaaaaaaa attcacttta tttcgactat aaaaaagtct 60
ctttctaata ttagtaataa taaatcaaag atttctggtg acctatattc cttttcacaa 120
gcatctgtat tttacaaatt atcacaaatc caagctatta agaagtatca tttgagatct 180
ctacttcaat atcgcgaagc atatcttatt cttaaggaaa gaatcaggaa tttttttgga 240
acacgaagaa tatttgattc caaatcaagg cataaaaaac ttcagaattc tggaatgaat 300
gaatggaaaa actggttaag gggtcattat caatacaatt tatctcagga tttatcgata 360
atcttttttg ttgattcaag gaaaagttgt acattgactc tagaaatatt aacagtactt 420
agaaagatat gtatgtatat tctttcgttg aaaaatatca aaaaatagtg ccatttgcgt 480
atgaatatga atcttttact tcttcttttt gatatctgcc aaatgggttt ctgcgattcc 540
gatcttttat caccacaact tgtatcgtta ggactaatat ttatatccgg agttagaaat 600
atttttcttt tgtcttttgc acccctttct atttgatttc tgattagggt tgttctatcg 660
ttttcctcct cccgctcttc aaaagaatcg atcaatttgt atgatcgtcg aggaatcttt 720
ttaccgattt cttctattcc aatagatttt ttttgaattg tttgattatt tggatcagtt 780
gtaattacat cgaatagaaa tttcaaacat tttgttttat tttctgaatc aaatcttctt 840
tgttcggata ttaaaacaag ctttttaaaa ttaagattaa aaccagttgt tgatttccta 900
gctaattcac tgatagaggt caagaaagga ccaatgtctg tcgataatga ttctccatta 960
aatatatcca ttttatgttc aagtttttgg taatcagtag gaagcatatc atgaatctta 1020
tttatccaaa ccattcctat agaatcttct gtccccattc cacctaaaat cctaggaatt 1080
cgttgatggt tggaatagat tcgtagaccg ggacgactga tacgctttaa aatatttcta 1140
tatgttcctt tcctattcct tctatgtcgc agggttgaaa ccaaaaaata tttgttgttt 1200
tccctatgtt tcctaacatt ttcaataaac ccttcttgta gaagtatttt aacaatgttt 1260
tcggcgatat tagtagatgc tattcgaacc gttccttttt tatccatgtt agcatttctt 1320
atagaagtta ttatatcggc aatagtgtcc ctacccatga cgaactaaaa ttatgggtgc 1380
cttccagttt tgatataatc aacatgttac tttttttttt tcatctacta gtatttataa 1440
gacttcagga gctaatgaga ctattttagt gaaattcaac tgtctcaatt cccgcgcgat 1500
cgctccaaaa actcgagttc cttttggatt tccttcttga tcaatgacaa ctgctgcatt 1560
gtcatcatat cgtattatca taccgttgtc gcgcttgagt tctttacatg tacgtacaat 1620
tacagctctg atcacttctg atctttcgag aggcatattg ggcactgctt ctttgattac 1680
agcaacaata acgtcaccaa tatgagcata tcgttgatta ctagctccta tgattcgaat 1740
acacatcaat tctcgagccc cgctgttgtc cgctacattc aaatgggtct gaggttgaat 1800
catatcattt tttttttaac aaagatcaaa gtgaaattca aaaaaaaaaa ctgggtgggc 1860
cattcagcgt atgttcgccc cctcgcccat attcatattg aaggttagtc agtcatttgg 1920
ataaacttta tgccccatat ctatgatatt tggattctca catgatgcat tctgagtcga 1980
aatgcgaaat aaaagagaag tctctacctt ccctaaagta aatataaata aagatagggt 2040
agacaaaatg actaattcta tgtggatcct gaatcctaaa aaacaggggg gttcttggta 2100
ttaattccat cgctggaatt aaagctcctg agactggggt gggacaaaat caaacaaaat 2160
agtaacaacg aattgatatg aacccatttt gctttgtact tatacaagac aggatagcat 2220
cccataagaa gatcctttta aattggcttt ggatatgatt catgatcccc agggcagaat 2280
taagacagat caaactaaag atattaagca taacaaacat tttgttcttg gagaaaattt 2340
cattattatt gggttattga tataagggga aaatgaagaa agaagggaaa gtaggccgca 2400
aaatctttct ttttctttga actccctcaa tcaaaaatcc ttcaattctc aaatgagaat 2460
agaaggaatc ataccttaca tacaatatct taattgaatt atatgtaatg atcaataaat 2520
tcattttgat tctacatcta catgtgtaga atcatagcaa caaccaattc aatagatatt 2580
ggggctggaa ttgacgaaca accaataccg atattagtgt ttatcagtgt cgaatagaaa 2640
taaaaatggg gcgtggccaa gtggtaaggc aacgggtttt ggtcccgtta ctcggaggt 2699
<210> 18
<211> 2698
<212> DNA
<213> Machilus thunbergii
<400> 18
gaagggattg atgaaacaaa tcaaaaaaaa attcaattta tttcgactat aaaaaagtct 60
ctttctaata ttagtaataa taaatcaaaa atttctggtg acccatattc cttttcacaa 120
gcatctgtat tttacaaatt atcacaaatc gaagctatca agaagtatca tttgagatct 180
ctacttcaat atcgcgaagc atatcttatt cttaaggata gaatcaggaa tttttttgga 240
acacgaagaa tatttgattc caaatcaagg cataaaaaac ttccgaattc tggaatgaat 300
gaatggaaaa actggttaag gggtcattat caatacaatt tatctcagga tttatcgata 360
atcttttttg ttgattcaag gaaaagttgt acattgactc tagaaatatt aaccgtacat 420
agaaagatat gtatgtatat tctttcgttg aaaaatgtca aaaaatagtg ccatttgcgt 480
atgaatatga atctgttact tcttcttttt gatatctgcc aaataggttt ctgcgattcc 540
gatcttttat caccacaact tgtatcgtta ggactaatat ttatatccgg agttagaaat 600
atttttcttt tgtcttttgc acccctttct atttgatttc tgattagggt tgttctatcg 660
ttttcctcct cccgctcttc aaaagaatcg atcaatttgt atgatcgtcg aggaatcttt 720
ttaccgattt cttctattcc aatagatttt ttttgaattg tttgattatt tggatcagtt 780
gtaattacat cgaatagaaa tttcaaacat ttttttttat tttctgaatc aaatcttctt 840
tgttcggata ttaaaacaag ctttttcaaa ttaagattaa aaccagttgt tgatttccta 900
gctaattcac tgatagaggt caagaaagga ccaatgtctg tcgataatga ttctccatta 960
aatatatcca ttttatgttc aagtttttgg taatcagtag gaagcatatc atgaatctta 1020
tttatccaaa ccattcctat ggaatcttct gtccccattc cacctaaaat cctaggaatt 1080
cgttgatggt tggaatagat tcgtagaccg ggacgactga tacgctttaa aatagttcta 1140
tatgttcctt tcctattcct tctatgtcgc agggttgaaa ccaaaaaata tttgttgttt 1200
tccctatgtt tcctaacatt ttcaataaac ccttcttgta gaagtatttt aacaatgttt 1260
tcggcgatat tagtagatgc tattcgaacc gttccttttt tatccatgtt agcatttctt 1320
atagaagtta ttatatcggc aatagtgtcc ctacccatga cgaactaaaa ttatgggtgc 1380
cttccagttt tgatacaatc aacatgttcc tttttttttt tcatctacta gtatttataa 1440
gacttcagga gctaatgaga ctattttagt gaaattcaac tgtctcaatt cccgcgcgat 1500
cgctccaaaa acgcgagttc cttttggatt tccttcttga tcaatgacaa ctgctgcatt 1560
gtcatcatat cgtattatca taccgttgtc gcgtttgagt tctttacatg tacgtacaat 1620
tacagctctg atcacttctg atctttcgag aggcatattg ggcactgctt ctttgattac 1680
agcaacaata acgtcaccaa tatgagcata tcgttgatta ctagctccta tgattcgaat 1740
acacatcaat tctcgagccc cgctgttgtc cgctacattc aaatgggtct gaggttgaat 1800
catatcattt tttttttaac aaagatcaaa gtgaaattca aaaaaaaaac tgggtgggcc 1860
attcagcgta tgttcgcccc ctcgcccata ttcatattga aggttagtta gtcatttgga 1920
taaactttat gtcccatatc tatgatattt ggattctcac atgatgcatt ctgagtcgaa 1980
atgcgaaata aaagagaagt ctcgaccttc cctaaagtaa atataaataa agatagggta 2040
gacaaaatga ctaattctat gtggatcctg aatcctaaaa aacggggggg ttcttggtat 2100
taattccagc gatggaatta aagctcctga gactggggtg ggacaaaatc aaacaaaata 2160
gtaacaacga attgatatga acccattttg ctttgtactt atacaagaca ggatagcatc 2220
ccataagaag atccttttaa attggctttg gatatgattc atgatcccca gggcagaatt 2280
aagacagatc aaactaaaga tattaagcat aacaaacatt ttgttcttgg agaaaatttc 2340
attattattg ggttattgat ataaggggaa aatgaagaaa gaagggaaag taggccgcaa 2400
aatatttctt tttctttgaa ctccctcaat caaaaatcct tcaattctca aatgagaata 2460
gaaggaatca taccttacat acaatatctt aattgaatta tatgtaatga tcaataaatt 2520
cattttgatt ctacatctac atgtgtaaaa tcatagcaac aaccaattca atagatattg 2580
gggctggaat tgacgaacaa ccaataccga tattagtgtt tatcggtgtc gaatagaaat 2640
aaaaatgggg cgtggccaag tggtaaggca acgggttttg gtcccgttac tcggaggt 2698
<210> 19
<211> 2698
<212> DNA
<213> Machilus thunbergii
<400> 19
gaagggattg atgaaacaaa tcaaaaaaaa attcaattta tttcgactat aaaaaagtct 60
ctttctaata ttagtaataa taaatcaaaa atttctggtg acccatattc cttttcacaa 120
gcatctgtat tttacaaatt atcacaaatc gaagctatca agaagtatca tttgagatct 180
ctacttcaat atcgcgaagc atatcttatt cttaaggata gaatcaggaa tttttttgga 240
acacgaagaa tatttgattc caaatcaagg cataaaaaac ttccgaattc tggaatgaat 300
gaatggaaaa actggttaag gggtcattat caatacaatt tatctcagga tttatcgata 360
atcttttttg ttgattcaag gaaaagttgt acattgactc tagaaatatt aaccgtacat 420
agaaagatat gtatgtatat tctttcgttg aaaaatgtca aaaaatagtg ccatttgcgt 480
atgaatatga atctgttact tcttcttttt gatatctgcc aaataggttt ctgcgattcc 540
gatcttttat caccacaact tgtatcgtta ggactaatat ttatatccgg agttagaaat 600
atttttcttt tgtcttttgc acccctttct atttgatttc tgattagggt tgttctatcg 660
ttttcctcct cccgctcttc aaaagaatcg atcaatttgt atgatcgtcg aggaatcttt 720
ttaccgattt cttctattcc aatagatttt ttttgaattg tttgattatt tggatcagtt 780
gtaattacat cgaatagaaa tttcaaacat ttttttttat tttctgaatc aaatcttctt 840
tgttcggata ttaaaacaag ctttttcaaa ttaagattaa aaccagttgt tgatttccta 900
gctaattcac tgatagaggt caagaaagga ccaatgtctg tcgataatga ttctccatta 960
aatatatcca ttttatgttc aagtttttgg taatcagtag gaagcatatc atgaatctta 1020
tttatccaaa ccattcctat ggaatcttct gtccccattc cacctaaaat cctaggaatt 1080
cgttgatggt tggaatagat tcgtagaccg ggacgactga tacgctttaa aatagttcta 1140
tatgttcctt tcctattcct tctatgtcgc agggttgaaa ccaaaaaata tttgttgttt 1200
tccctatgtt tcctaacatt ttcaataaac ccttcttgta gaagtatttt aacaatgttt 1260
tcggcgatat tagtagatgc tattcgaacc gttccttttt tatccatgtt agcatttctt 1320
atagaagtta ttatatcggc aatagtgtcc ctacccatga cgaactaaaa ttatgggtgc 1380
cttccagttt tgatacaatc aacatgttcc tttttttttt tcatctacta gtatttataa 1440
gacttcagga gctaatgaga ctattttagt gaaattcaac tgtctcaatt cccgcgcgat 1500
cgctccaaaa acgcgagttc cttttggatt tccttcttga tcaatgacaa ctgctgcatt 1560
gtcatcatat cgtattatca taccgttgtc gcgtttgagt tctttacatg tacgtacaat 1620
tacagctctg atcacttctg atctttcgag aggcatattg ggcactgctt ctttgattac 1680
agcaacaata acgtcaccaa tatgagcata tcgttgatta ctagctccta tgattcgaat 1740
acacatcaat tctcgagccc cgctgttgtc cgctacattc aaatgggtct gaggttgaat 1800
catatcattt tttttttaac aaagatcaaa gtgaaattca aaaaaaaaac tgggtgggcc 1860
attcagcgta tgttcgcccc ctcgcccata ttcatattga aggttagtta gtcatttgga 1920
taaactttat gtcccatatc tatgatattt ggattctcac atgatgcatt ctgagtcgaa 1980
atgcgaaata aaagagaagt ctcgaccttc cctaaagtaa atataaataa agatagggta 2040
gacaaaatga ctaattctat gtggatcctg aatcctaaaa aacggggggg ttcttggtat 2100
taattccagc gatggaatta aagctcctga gactggggtg ggacaaaatc aaacaaaata 2160
gtaacaacga attgatatga acccattttg ctttgtactt atacaagaca ggatagcatc 2220
ccataagaag atccttttaa attggctttg gatatgattc atgatcccca gggcagaatt 2280
aagacagatc aaactaaaga tattaagcat aacaaacatt ttgttcttgg agaaaatttc 2340
attattattg ggttattgat ataaggggaa aatgaagaaa gaagggaaag taggccgcaa 2400
aatatttctt tttctttgaa ctccctcaat caaaaatcct tcaattctca aatgagaata 2460
gaaggaatca taccttacat acaatatctt aattgaatta tatgtaatga tcaataaatt 2520
cattttgatt ctacatctac atgtgtaaaa tcatagcaac aaccaattca atagatattg 2580
gggctggaat tgacgaacaa ccaataccga tattagtgtt tatcggtgtc gaatagaaat 2640
aaaaatgggg cgtggccaag tggtaaggca acgggttttg gtcccgttac tcggaggt 2698
<210> 20
<211> 2698
<212> DNA
<213> Machilus thunbergii
<400> 20
gaagggattg atgaaacaaa tcaaaaaaaa attcaattta tttcgactat aaaaaagtct 60
ctttctaata ttagtaataa taaatcaaaa atttctggtg acccatattc cttttcacaa 120
gcatctgtat tttacaaatt atcacaaatc gaagctatca agaagtatca tttgagatct 180
ctacttcaat atcgcgaagc atatcttatt cttaaggata gaatcaggaa tttttttgga 240
acacgaagaa tatttgattc caaatcaagg cataaaaaac ttccgaattc tggaatgaat 300
gaatggaaaa actggttaag gggtcattat caatacaatt tatctcagga tttatcgata 360
atcttttttg ttgattcaag gaaaagttgt acattgactc tagaaatatt aaccgtacat 420
agaaagatat gtatgtatat tctttcgttg aaaaatgtca aaaaatagtg ccatttgcgt 480
atgaatatga atctgttact tcttcttttt gatatctgcc aaataggttt ctgcgattcc 540
gatcttttat caccacaact tgtatcgtta ggactaatat ttatatccgg agttagaaat 600
atttttcttt tgtcttttgc acccctttct atttgatttc tgattagggt tgttctatcg 660
ttttcctcct cccgctcttc aaaagaatcg atcaatttgt atgatcgtcg aggaatcttt 720
ttaccgattt cttctattcc aatagatttt ttttgaattg tttgattatt tggatcagtt 780
gtaattacat cgaatagaaa tttcaaacat ttttttttat tttctgaatc aaatcttctt 840
tgttcggata ttaaaacaag ctttttcaaa ttaagattaa aaccagttgt tgatttccta 900
gctaattcac tgatagaggt caagaaagga ccaatgtctg tcgataatga ttctccatta 960
aatatatcca ttttatgttc aagtttttgg taatcagtag gaagcatatc atgaatctta 1020
tttatccaaa ccattcctat ggaatcttct gtccccattc cacctaaaat cctaggaatt 1080
cgttgatggt tggaatagat tcgtagaccg ggacgactga tacgctttaa aatagttcta 1140
tatgttcctt tcctattcct tctatgtcgc agggttgaaa ccaaaaaata tttgttgttt 1200
tccctatgtt tcctaacatt ttcaataaac ccttcttgta gaagtatttt aacaatgttt 1260
tcggcgatat tagtagatgc tattcgaacc gttccttttt tatccatgtt agcatttctt 1320
atagaagtta ttatatcggc aatagtgtcc ctacccatga cgaactaaaa ttatgggtgc 1380
cttccagttt tgatacaatc aacatgttcc tttttttttt tcatctacta gtatttataa 1440
gacttcagga gctaatgaga ctattttagt gaaattcaac tgtctcaatt cccgcgcgat 1500
cgctccaaaa acgcgagttc cttttggatt tccttcttga tcaatgacaa ctgctgcatt 1560
gtcatcatat cgtattatca taccgttgtc gcgtttgagt tctttacatg tacgtacaat 1620
tacagctctg atcacttctg atctttcgag aggcatattg ggcactgctt ctttgattac 1680
agcaacaata acgtcaccaa tatgagcata tcgttgatta ctagctccta tgattcgaat 1740
acacatcaat tctcgagccc cgctgttgtc cgctacattc aaatgggtct gaggttgaat 1800
catatcattt tttttttaac aaagatcaaa gtgaaattca aaaaaaaaac tgggtgggcc 1860
attcagcgta tgttcgcccc ctcgcccata ttcatattga aggttagtta gtcatttgga 1920
taaactttat gtcccatatc tatgatattt ggattctcac atgatgcatt ctgagtcgaa 1980
atgcgaaata aaagagaagt ctcgaccttc cctaaagtaa atataaataa agatagggta 2040
gacaaaatga ctaattctat gtggatcctg aatcctaaaa aacggggggg ttcttggtat 2100
taattccagc gatggaatta aagctcctga gactggggtg ggacaaaatc aaacaaaata 2160
gtaacaacga attgatatga acccattttg ctttgtactt atacaagaca ggatagcatc 2220
ccataagaag atccttttaa attggctttg gatatgattc atgatcccca gggcagaatt 2280
aagacagatc aaactaaaga tattaagcat aacaaacatt ttgttcttgg agaaaatttc 2340
attattattg ggttattgat ataaggggaa aatgaagaaa gaagggaaag taggccgcaa 2400
aatatttctt tttctttgaa ctccctcaat caaaaatcct tcaattctca aatgagaata 2460
gaaggaatca taccttacat acaatatctt aattgaatta tatgtaatga tcaataaatt 2520
cattttgatt ctacatctac atgtgtaaaa tcatagcaac aaccaattca atagatattg 2580
gggctggaat tgacgaacaa ccaataccga tattagtgtt tatcggtgtc gaatagaaat 2640
aaaaatgggg cgtggccaag tggtaaggca acgggttttg gtcccgttac tcggaggt 2698
<210> 21
<211> 2698
<212> DNA
<213> Machilus thunbergii
<400> 21
gaagggattg atgaaacaaa tcaaaaaaaa attcaattta tttcgactat aaaaaagtct 60
ctttctaata ttagtaataa taaatcaaaa atttctggtg acccatattc cttttcacaa 120
gcatctgtat tttacaaatt atcacaaatc gaagctatca agaagtatca tttgagatct 180
ctacttcaat atcgcgaagc atatcttatt cttaaggata gaatcaggaa tttttttgga 240
acacgaagaa tatttgattc caaatcaagg cataaaaaac ttccgaattc tggaatgaat 300
gaatggaaaa actggttaag gggtcattat caatacaatt tatctcagga tttatcgata 360
atcttttttg ttgattcaag gaaaagttgt acattgactc tagaaatatt aaccgtacat 420
agaaagatat gtatgtatat tctttcgttg aaaaatgtca aaaaatagtg ccatttgcgt 480
atgaatatga atctgttact tcttcttttt gatatctgcc aaataggttt ctgcgattcc 540
gatcttttat caccacaact tgtatcgtta ggactaatat ttatatccgg agttagaaat 600
atttttcttt tgtcttttgc acccctttct atttgatttc tgattagggt tgttctatcg 660
ttttcctcct cccgctcttc aaaagaatcg atcaatttgt atgatcgtcg aggaatcttt 720
ttaccgattt cttctattcc aatagatttt ttttgaattg tttgattatt tggatcagtt 780
gtaattacat cgaatagaaa tttcaaacat ttttttttat tttctgaatc aaatcttctt 840
tgttcggata ttaaaacaag ctttttcaaa ttaagattaa aaccagttgt tgatttccta 900
gctaattcac tgatagaggt caagaaagga ccaatgtctg tcgataatga ttctccatta 960
aatatatcca ttttatgttc aagtttttgg taatcagtag gaagcatatc atgaatctta 1020
tttatccaaa ccattcctat ggaatcttct gtccccattc cacctaaaat cctaggaatt 1080
cgttgatggt tggaatagat tcgtagaccg ggacgactga tacgctttaa aatagttcta 1140
tatgttcctt tcctattcct tctatgtcgc agggttgaaa ccaaaaaata tttgttgttt 1200
tccctatgtt tcctaacatt ttcaataaac ccttcttgta gaagtatttt aacaatgttt 1260
tcggcgatat tagtagatgc tattcgaacc gttccttttt tatccatgtt agcatttctt 1320
atagaagtta ttatatcggc aatagtgtcc ctacccatga cgaactaaaa ttatgggtgc 1380
cttccagttt tgatacaatc aacatgttcc tttttttttt tcatctacta gtatttataa 1440
gacttcagga gctaatgaga ctattttagt gaaattcaac tgtctcaatt cccgcgcgat 1500
cgctccaaaa acgcgagttc cttttggatt tccttcttga tcaatgacaa ctgctgcatt 1560
gtcatcatat cgtattatca taccgttgtc gcgtttgagt tctttacatg tacgtacaat 1620
tacagctctg atcacttctg atctttcgag aggcatattg ggcactgctt ctttgattac 1680
agcaacaata acgtcaccaa tatgagcata tcgttgatta ctagctccta tgattcgaat 1740
acacatcaat tctcgagccc cgctgttgtc cgctacattc aaatgggtct gaggttgaat 1800
catatcattt tttttttaac aaagatcaaa gtgaaattca aaaaaaaaac tgggtgggcc 1860
attcagcgta tgttcgcccc ctcgcccata ttcatattga aggttagtta gtcatttgga 1920
taaactttat gtcccatatc tatgatattt ggattctcac atgatgcatt ctgagtcgaa 1980
atgcgaaata aaagagaagt ctcgaccttc cctaaagtaa atataaataa agatagggta 2040
gacaaaatga ctaattctat gtggatcctg aatcctaaaa aacggggggg ttcttggtat 2100
taattccagc gatggaatta aagctcctga gactggggtg ggacaaaatc aaacaaaata 2160
gtaacaacga attgatatga acccattttg ctttgtactt atacaagaca ggatagcatc 2220
ccataagaag atccttttaa attggctttg gatatgattc atgatcccca gggcagaatt 2280
aagacagatc aaactaaaga tattaagcat aacaaacatt ttgttcttgg agaaaatttc 2340
attattattg ggttattgat ataaggggaa aatgaagaaa gaagggaaag taggccgcaa 2400
aatatttctt tttctttgaa ctccctcaat caaaaatcct tcaattctca aatgagaata 2460
gaaggaatca taccttacat acaatatctt aattgaatta tatgtaatga tcaataaatt 2520
cattttgatt ctacatctac atgtgtaaaa tcatagcaac aaccaattca atagatattg 2580
gggctggaat tgacgaacaa ccaataccga tattagtgtt tatcggtgtc gaatagaaat 2640
aaaaatgggg cgtggccaag tggtaaggca acgggttttg gtcccgttac tcggaggt 2698
<210> 22
<211> 2698
<212> DNA
<213> Machilus thunbergii
<400> 22
gaagggattg atgaaacaaa tcaaaaaaaa attcaattta tttcgactat aaaaaagtct 60
ctttctaata ttagtaataa taaatcaaaa atttctggtg acccatattc cttttcacaa 120
gcatctgtat tttacaaatt atcacaaatc gaagctatca agaagtatca tttgagatct 180
ctacttcaat atcgcgaagc atatcttatt cttaaggata gaatcaggaa tttttttgga 240
acacgaagaa tatttgattc caaatcaagg cataaaaaac ttccgaattc tggaatgaat 300
gaatggaaaa actggttaag gggtcattat caatacaatt tatctcagga tttatcgata 360
atcttttttg ttgattcaag gaaaagttgt acattgactc tagaaatatt aaccgtacat 420
agaaagatat gtatgtatat tctttcgttg aaaaatgtca aaaaatagtg ccatttgcgt 480
atgaatatga atctgttact tcttcttttt gatatctgcc aaataggttt ctgcgattcc 540
gatcttttat caccacaact tgtatcgtta ggactaatat ttatatccgg agttagaaat 600
atttttcttt tgtcttttgc acccctttct atttgatttc tgattagggt tgttctatcg 660
ttttcctcct cccgctcttc aaaagaatcg atcaatttgt atgatcgtcg aggaatcttt 720
ttaccgattt cttctattcc aatagatttt ttttgaattg tttgattatt tggatcagtt 780
gtaattacat cgaatagaaa tttcaaacat ttttttttat tttctgaatc aaatcttctt 840
tgttcggata ttaaaacaag ctttttcaaa ttaagattaa aaccagttgt tgatttccta 900
gctaattcac tgatagaggt caagaaagga ccaatgtctg tcgataatga ttctccatta 960
aatatatcca ttttatgttc aagtttttgg taatcagtag gaagcatatc atgaatctta 1020
tttatccaaa ccattcctat ggaatcttct gtccccattc cacctaaaat cctaggaatt 1080
cgttgatggt tggaatagat tcgtagaccg ggacgactga tacgctttaa aatagttcta 1140
tatgttcctt tcctattcct tctatgtcgc agggttgaaa ccaaaaaata tttgttgttt 1200
tccctatgtt tcctaacatt ttcaataaac ccttcttgta gaagtatttt aacaatgttt 1260
tcggcgatat tagtagatgc tattcgaacc gttccttttt tatccatgtt agcatttctt 1320
atagaagtta ttatatcggc aatagtgtcc ctacccatga cgaactaaaa ttatgggtgc 1380
cttccagttt tgatacaatc aacatgttcc tttttttttt tcatctacta gtatttataa 1440
gacttcagga gctaatgaga ctattttagt gaaattcaac tgtctcaatt cccgcgcgat 1500
cgctccaaaa acgcgagttc cttttggatt tccttcttga tcaatgacaa ctgctgcatt 1560
gtcatcatat cgtattatca taccgttgtc gcgtttgagt tctttacatg tacgtacaat 1620
tacagctctg atcacttctg atctttcgag aggcatattg ggcactgctt ctttgattac 1680
agcaacaata acgtcaccaa tatgagcata tcgttgatta ctagctccta tgattcgaat 1740
acacatcaat tctcgagccc cgctgttgtc cgctacattc aaatgggtct gaggttgaat 1800
catatcattt tttttttaac aaagatcaaa gtgaaattca aaaaaaaaac tgggtgggcc 1860
attcagcgta tgttcgcccc ctcgcccata ttcatattga aggttagtta gtcatttgga 1920
taaactttat gtcccatatc tatgatattt ggattctcac atgatgcatt ctgagtcgaa 1980
atgcgaaata aaagagaagt ctcgaccttc cctaaagtaa atataaataa agatagggta 2040
gacaaaatga ctaattctat gtggatcctg aatcctaaaa aacggggggg ttcttggtat 2100
taattccagc gatggaatta aagctcctga gactggggtg ggacaaaatc aaacaaaata 2160
gtaacaacga attgatatga acccattttg ctttgtactt atacaagaca ggatagcatc 2220
ccataagaag atccttttaa attggctttg gatatgattc atgatcccca gggcagaatt 2280
aagacagatc aaactaaaga tattaagcat aacaaacatt ttgttcttgg agaaaatttc 2340
attattattg ggttattgat ataaggggaa aatgaagaaa gaagggaaag taggccgcaa 2400
aatatttctt tttctttgaa ctccctcaat caaaaatcct tcaattctca aatgagaata 2460
gaaggaatca taccttacat acaatatctt aattgaatta tatgtaatga tcaataaatt 2520
cattttgatt ctacatctac atgtgtaaaa tcatagcaac aaccaattca atagatattg 2580
gggctggaat tgacgaacaa ccaataccga tattagtgtt tatcggtgtc gaatagaaat 2640
aaaaatgggg cgtggccaag tggtaaggca acgggttttg gtcccgttac tcggaggt 2698
<210> 23
<211> 2697
<212> DNA
<213> Machilus leptophylla
<400> 23
gaagggattg atgaaacaaa taaaaaaaaa attcaattta tttcgactat aaaaaagtat 60
ctttctaata ttagtaataa taaatcaaaa atttctggtg acccatattc cttttcacaa 120
gcatctgtat tttacaaatt atcacaaatc gaagctatca agaagtatca tttgagatct 180
ctacttcaat atcgcgaagc atatcttatt cttaaggata gaatcaggaa tttttttgga 240
acacgaagaa tatttgattc caaatcaagg cataaaaaac ttccgaattc tggaatgaat 300
gaatggaaaa actggttaag gggtcattat caatacaatt tatctcagga tttatcgata 360
atattttttg ttgattcaag gaaaagttgt acattgactc tagaaatatt aaccgtacat 420
agaaagatat gtatgtatat tctttcgttg aaaaatgtca aaaaatagtg ccatttgcgt 480
atgaatatga atctgttact tcttcttttt gatatctgcc aaataggttt ctgcgattcc 540
gatcttttat caccacaact tgtatcgtta ggactaatat ttatatccgg agttagaaat 600
atttttcttt tgtcttttgc acccctttct atttgatttc tgattagggt tgttctatcg 660
ttttcctcct cccgctcttc aaaagaatcg atcaatttgt atgatcgtcg aggaatcttt 720
ttaccgattt cttctattcc aatagatttt ttttgaattg tttgattatt tggatcagtt 780
gtaattacat cgaatagaaa tttcaaacat ttttttttat tttctgaatc aaatcttctt 840
tgttcggata ttaaaacaag ctttttcaaa ttaagattaa aaccagttgt tgatttccta 900
gctaattcac tgatagaggt caagaaagga ccaatgtctg tcgataatga ttctccatta 960
aatatatcca ttttatgttc aagtttttgg taatcagtag gaagcatatc atgaatctta 1020
tttatccaaa ccattcctat ggaatcttct gtccccattc cacctaaaat cctaggaatt 1080
cgttgatggt tggaatagat tcgtagaccg ggacgactga tacgctttaa aatagttcta 1140
tatgttcctt tcctattcct tctatgtcgc agggttgaaa ccaaaaaata tttgttgttt 1200
tccctatgtt tcctaacatt ttcaataaac ccttcttgta gaagtatttt aacaatgttt 1260
tcggcgatat tagtagatgc tattcgaacc gttccttttt tatccatgtt agcatttctt 1320
atagaagtta ttatatcggc aatagtgtcc ctacccatga cgaactaaaa ttatgggtgc 1380
cttccagttt tgatacaatc aacatgttcc tttttttttt tcatctacta gtatttataa 1440
gacttcagga gctaatgaga ctattttagt gaaattcaac tgtctcaatt cccgcgcgat 1500
cgctccaaaa acgcgagttc cttttggatt tccttcttga tcaatgacaa ctgctgcatt 1560
gtcatcatat cgtattatca taccgttgtc gcgtttgagt tctttacatg tacgtacaat 1620
tacagctctg atcacttctg atctttcgag aggcatattg ggcactgctt ctttgattac 1680
agcaacaata acgtcaccaa tatgagcata tcgttgatta ctagctccta tgattcgaat 1740
acacatcaat tctcgagccc cgctgttgtc cgctacattc aaatgggtct gaggttgaat 1800
catatcattt tttttttaac aaagatcaaa gtgaaattca aaaaaaaact gggtgggcca 1860
ttcagcgtat gttcgccccc tcgcccatat tcatattgaa ggttagttag tcatttggat 1920
aaactttatg tcccatatct atgatatttg gattctcaca tgatgcattc tgagtcgaaa 1980
tgcgaaataa aagagaagtc tcgaccttcc ctaaagtaaa tataaataaa gatagggtag 2040
acaaaatgac taattctatg tggatcctga atcctaaaaa acgggggggt tcttggtatt 2100
aattccagcg atggaattaa agctcctgag actggggtgg gacaaaatca aacaaaatag 2160
taacaacgaa ttgatatgaa cccattttgc tttgtactta tacaagacag gatagcatcc 2220
cataagaaga tccttttaaa ttggctttgg atatgattca tgatccccag ggcagaatta 2280
agacagatca aactaaagat attaagcata acaaacattt tgttcttgga gaaaatttca 2340
ttattattgg gttattgata taaggggaaa atgaagaaag aagggaaagt aggccgcaaa 2400
atatttcttt ttctttgaac tccctcaatc aaaaatcctt caattctcaa atgagaatag 2460
aaggaatcat accttacata caatatctta attgaattat atgtaatgat caataaattc 2520
attttgattc tacatctaca tgtgtaaaat catagcaaca accaattcaa tagatattgg 2580
ggctggaatt gacgaacaac caataccgat attagtgttt atcggtgtcg aatagaaata 2640
aaaatggggc gtggccaagt ggtaaggcaa cgggttttgg tcccgttact cggaggt 2697
<210> 24
<211> 2697
<212> DNA
<213> Machilus leptophylla
<400> 24
gaagggattg atgaaacaaa taaaaaaaaa attcaattta tttcgactat aaaaaagtat 60
ctttctaata ttagtaataa taaatcaaaa atttctggtg acccatattc cttttcacaa 120
gcatctgtat tttacaaatt atcacaaatc gaagctatca agaagtatca tttgagatct 180
ctacttcaat atcgcgaagc atatcttatt cttaaggata gaatcaggaa tttttttgga 240
acacgaagaa tatttgattc caaatcaagg cataaaaaac ttccgaattc tggaatgaat 300
gaatggaaaa actggttaag gggtcattat caatacaatt tatctcagga tttatcgata 360
atattttttg ttgattcaag gaaaagttgt acattgactc tagaaatatt aaccgtacat 420
agaaagatat gtatgtatat tctttcgttg aaaaatgtca aaaaatagtg ccatttgcgt 480
atgaatatga atctgttact tcttcttttt gatatctgcc aaataggttt ctgcgattcc 540
gatcttttat caccacaact tgtatcgtta ggactaatat ttatatccgg agttagaaat 600
atttttcttt tgtcttttgc acccctttct atttgatttc tgattagggt tgttctatcg 660
ttttcctcct cccgctcttc aaaagaatcg atcaatttgt atgatcgtcg aggaatcttt 720
ttaccgattt cttctattcc aatagatttt ttttgaattg tttgattatt tggatcagtt 780
gtaattacat cgaatagaaa tttcaaacat ttttttttat tttctgaatc aaatcttctt 840
tgttcggata ttaaaacaag ctttttcaaa ttaagattaa aaccagttgt tgatttccta 900
gctaattcac tgatagaggt caagaaagga ccaatgtctg tcgataatga ttctccatta 960
aatatatcca ttttatgttc aagtttttgg taatcagtag gaagcatatc atgaatctta 1020
tttatccaaa ccattcctat ggaatcttct gtccccattc cacctaaaat cctaggaatt 1080
cgttgatggt tggaatagat tcgtagaccg ggacgactga tacgctttaa aatagttcta 1140
tatgttcctt tcctattcct tctatgtcgc agggttgaaa ccaaaaaata tttgttgttt 1200
tccctatgtt tcctaacatt ttcaataaac ccttcttgta gaagtatttt aacaatgttt 1260
tcggcgatat tagtagatgc tattcgaacc gttccttttt tatccatgtt agcatttctt 1320
atagaagtta ttatatcggc aatagtgtcc ctacccatga cgaactaaaa ttatgggtgc 1380
cttccagttt tgatacaatc aacatgttcc tttttttttt tcatctacta gtatttataa 1440
gacttcagga gctaatgaga ctattttagt gaaattcaac tgtctcaatt cccgcgcgat 1500
cgctccaaaa acgcgagttc cttttggatt tccttcttga tcaatgacaa ctgctgcatt 1560
gtcatcatat cgtattatca taccgttgtc gcgtttgagt tctttacatg tacgtacaat 1620
tacagctctg atcacttctg atctttcgag aggcatattg ggcactgctt ctttgattac 1680
agcaacaata acgtcaccaa tatgagcata tcgttgatta ctagctccta tgattcgaat 1740
acacatcaat tctcgagccc cgctgttgtc cgctacattc aaatgggtct gaggttgaat 1800
catatcattt tttttttaac aaagatcaaa gtgaaattca aaaaaaaact gggtgggcca 1860
ttcagcgtat gttcgccccc tcgcccatat tcatattgaa ggttagttag tcatttggat 1920
aaactttatg tcccatatct atgatatttg gattctcaca tgatgcattc tgagtcgaaa 1980
tgcgaaataa aagagaagtc tcgaccttcc ctaaagtaaa tataaataaa gatagggtag 2040
acaaaatgac taattctatg tggatcctga atcctaaaaa acgggggggt tcttggtatt 2100
aattccagcg atggaattaa agctcctgag actggggtgg gacaaaatca aacaaaatag 2160
taacaacgaa ttgatatgaa cccattttgc tttgtactta tacaagacag gatagcatcc 2220
cataagaaga tccttttaaa ttggctttgg atatgattca tgatccccag ggcagaatta 2280
agacagatca aactaaagat attaagcata acaaacattt tgttcttgga gaaaatttca 2340
ttattattgg gttattgata taaggggaaa atgaagaaag aagggaaagt aggccgcaaa 2400
atatttcttt ttctttgaac tccctcaatc aaaaatcctt caattctcaa atgagaatag 2460
aaggaatcat accttacata caatatctta attgaattat atgtaatgat caataaattc 2520
attttgattc tacatctaca tgtgtaaaat catagcaaca accaattcaa tagatattgg 2580
ggctggaatt gacgaacaac caataccgat attagtgttt atcggtgtcg aatagaaata 2640
aaaatggggc gtggccaagt ggtaaggcaa cgggttttgg tcccgttact cggaggt 2697
<210> 25
<211> 2697
<212> DNA
<213> Machilus leptophylla
<400> 25
gaagggattg atgaaacaaa taaaaaaaaa attcaattta tttcgactat aaaaaagtat 60
ctttctaata ttagtaataa taaatcaaaa atttctggtg acccatattc cttttcacaa 120
gcatctgtat tttacaaatt atcacaaatc gaagctatca agaagtatca tttgagatct 180
ctacttcaat atcgcgaagc atatcttatt cttaaggata gaatcaggaa tttttttgga 240
acacgaagaa tatttgattc caaatcaagg cataaaaaac ttccgaattc tggaatgaat 300
gaatggaaaa actggttaag gggtcattat caatacaatt tatctcagga tttatcgata 360
atattttttg ttgattcaag gaaaagttgt acattgactc tagaaatatt aaccgtacat 420
agaaagatat gtatgtatat tctttcgttg aaaaatgtca aaaaatagtg ccatttgcgt 480
atgaatatga atctgttact tcttcttttt gatatctgcc aaataggttt ctgcgattcc 540
gatcttttat caccacaact tgtatcgtta ggactaatat ttatatccgg agttagaaat 600
atttttcttt tgtcttttgc acccctttct atttgatttc tgattagggt tgttctatcg 660
ttttcctcct cccgctcttc aaaagaatcg atcaatttgt atgatcgtcg aggaatcttt 720
ttaccgattt cttctattcc aatagatttt ttttgaattg tttgattatt tggatcagtt 780
gtaattacat cgaatagaaa tttcaaacat ttttttttat tttctgaatc aaatcttctt 840
tgttcggata ttaaaacaag ctttttcaaa ttaagattaa aaccagttgt tgatttccta 900
gctaattcac tgatagaggt caagaaagga ccaatgtctg tcgataatga ttctccatta 960
aatatatcca ttttatgttc aagtttttgg taatcagtag gaagcatatc atgaatctta 1020
tttatccaaa ccattcctat ggaatcttct gtccccattc cacctaaaat cctaggaatt 1080
cgttgatggt tggaatagat tcgtagaccg ggacgactga tacgctttaa aatagttcta 1140
tatgttcctt tcctattcct tctatgtcgc agggttgaaa ccaaaaaata tttgttgttt 1200
tccctatgtt tcctaacatt ttcaataaac ccttcttgta gaagtatttt aacaatgttt 1260
tcggcgatat tagtagatgc tattcgaacc gttccttttt tatccatgtt agcatttctt 1320
atagaagtta ttatatcggc aatagtgtcc ctacccatga cgaactaaaa ttatgggtgc 1380
cttccagttt tgatacaatc aacatgttcc tttttttttt tcatctacta gtatttataa 1440
gacttcagga gctaatgaga ctattttagt gaaattcaac tgtctcaatt cccgcgcgat 1500
cgctccaaaa acgcgagttc cttttggatt tccttcttga tcaatgacaa ctgctgcatt 1560
gtcatcatat cgtattatca taccgttgtc gcgtttgagt tctttacatg tacgtacaat 1620
tacagctctg atcacttctg atctttcgag aggcatattg ggcactgctt ctttgattac 1680
agcaacaata acgtcaccaa tatgagcata tcgttgatta ctagctccta tgattcgaat 1740
acacatcaat tctcgagccc cgctgttgtc cgctacattc aaatgggtct gaggttgaat 1800
catatcattt tttttttaac aaagatcaaa gtgaaattca aaaaaaaact gggtgggcca 1860
ttcagcgtat gttcgccccc tcgcccatat tcatattgaa ggttagttag tcatttggat 1920
aaactttatg tcccatatct atgatatttg gattctcaca tgatgcattc tgagtcgaaa 1980
tgcgaaataa aagagaagtc tcgaccttcc ctaaagtaaa tataaataaa gatagggtag 2040
acaaaatgac taattctatg tggatcctga atcctaaaaa acgggggggt tcttggtatt 2100
aattccagcg atggaattaa agctcctgag actggggtgg gacaaaatca aacaaaatag 2160
taacaacgaa ttgatatgaa cccattttgc tttgtactta tacaagacag gatagcatcc 2220
cataagaaga tccttttaaa ttggctttgg atatgattca tgatccccag ggcagaatta 2280
agacagatca aactaaagat attaagcata acaaacattt tgttcttgga gaaaatttca 2340
ttattattgg gttattgata taaggggaaa atgaagaaag aagggaaagt aggccgcaaa 2400
atatttcttt ttctttgaac tccctcaatc aaaaatcctt caattctcaa atgagaatag 2460
aaggaatcat accttacata caatatctta attgaattat atgtaatgat caataaattc 2520
attttgattc tacatctaca tgtgtaaaat catagcaaca accaattcaa tagatattgg 2580
ggctggaatt gacgaacaac caataccgat attagtgttt atcggtgtcg aatagaaata 2640
aaaatggggc gtggccaagt ggtaaggcaa cgggttttgg tcccgttact cggaggt 2697
<210> 26
<211> 2697
<212> DNA
<213> Machilus leptophylla
<400> 26
gaagggattg atgaaacaaa taaaaaaaaa attcaattta tttcgactat aaaaaagtat 60
ctttctaata ttagtaataa taaatcaaaa atttctggtg acccatattc cttttcacaa 120
gcatctgtat tttacaaatt atcacaaatc gaagctatca agaagtatca tttgagatct 180
ctacttcaat atcgcgaagc atatcttatt cttaaggata gaatcaggaa tttttttgga 240
acacgaagaa tatttgattc caaatcaagg cataaaaaac ttccgaattc tggaatgaat 300
gaatggaaaa actggttaag gggtcattat caatacaatt tatctcagga tttatcgata 360
atattttttg ttgattcaag gaaaagttgt acattgactc tagaaatatt aaccgtacat 420
agaaagatat gtatgtatat tctttcgttg aaaaatgtca aaaaatagtg ccatttgcgt 480
atgaatatga atctgttact tcttcttttt gatatctgcc aaataggttt ctgcgattcc 540
gatcttttat caccacaact tgtatcgtta ggactaatat ttatatccgg agttagaaat 600
atttttcttt tgtcttttgc acccctttct atttgatttc tgattagggt tgttctatcg 660
ttttcctcct cccgctcttc aaaagaatcg atcaatttgt atgatcgtcg aggaatcttt 720
ttaccgattt cttctattcc aatagatttt ttttgaattg tttgattatt tggatcagtt 780
gtaattacat cgaatagaaa tttcaaacat ttttttttat tttctgaatc aaatcttctt 840
tgttcggata ttaaaacaag ctttttcaaa ttaagattaa aaccagttgt tgatttccta 900
gctaattcac tgatagaggt caagaaagga ccaatgtctg tcgataatga ttctccatta 960
aatatatcca ttttatgttc aagtttttgg taatcagtag gaagcatatc atgaatctta 1020
tttatccaaa ccattcctat ggaatcttct gtccccattc cacctaaaat cctaggaatt 1080
cgttgatggt tggaatagat tcgtagaccg ggacgactga tacgctttaa aatagttcta 1140
tatgttcctt tcctattcct tctatgtcgc agggttgaaa ccaaaaaata tttgttgttt 1200
tccctatgtt tcctaacatt ttcaataaac ccttcttgta gaagtatttt aacaatgttt 1260
tcggcgatat tagtagatgc tattcgaacc gttccttttt tatccatgtt agcatttctt 1320
atagaagtta ttatatcggc aatagtgtcc ctacccatga cgaactaaaa ttatgggtgc 1380
cttccagttt tgatacaatc aacatgttcc tttttttttt tcatctacta gtatttataa 1440
gacttcagga gctaatgaga ctattttagt gaaattcaac tgtctcaatt cccgcgcgat 1500
cgctccaaaa acgcgagttc cttttggatt tccttcttga tcaatgacaa ctgctgcatt 1560
gtcatcatat cgtattatca taccgttgtc gcgtttgagt tctttacatg tacgtacaat 1620
tacagctctg atcacttctg atctttcgag aggcatattg ggcactgctt ctttgattac 1680
agcaacaata acgtcaccaa tatgagcata tcgttgatta ctagctccta tgattcgaat 1740
acacatcaat tctcgagccc cgctgttgtc cgctacattc aaatgggtct gaggttgaat 1800
catatcattt tttttttaac aaagatcaaa gtgaaattca aaaaaaaact gggtgggcca 1860
ttcagcgtat gttcgccccc tcgcccatat tcatattgaa ggttagttag tcatttggat 1920
aaactttatg tcccatatct atgatatttg gattctcaca tgatgcattc tgagtcgaaa 1980
tgcgaaataa aagagaagtc tcgaccttcc ctaaagtaaa tataaataaa gatagggtag 2040
acaaaatgac taattctatg tggatcctga atcctaaaaa acgggggggt tcttggtatt 2100
aattccagcg atggaattaa agctcctgag actggggtgg gacaaaatca aacaaaatag 2160
taacaacgaa ttgatatgaa cccattttgc tttgtactta tacaagacag gatagcatcc 2220
cataagaaga tccttttaaa ttggctttgg atatgattca tgatccccag ggcagaatta 2280
agacagatca aactaaagat attaagcata acaaacattt tgttcttgga gaaaatttca 2340
ttattattgg gttattgata taaggggaaa atgaagaaag aagggaaagt aggccgcaaa 2400
atatttcttt ttctttgaac tccctcaatc aaaaatcctt caattctcaa atgagaatag 2460
aaggaatcat accttacata caatatctta attgaattat atgtaatgat caataaattc 2520
attttgattc tacatctaca tgtgtaaaat catagcaaca accaattcaa tagatattgg 2580
ggctggaatt gacgaacaac caataccgat attagtgttt atcggtgtcg aatagaaata 2640
aaaatggggc gtggccaagt ggtaaggcaa cgggttttgg tcccgttact cggaggt 2697
<210> 27
<211> 2697
<212> DNA
<213> Machilus leptophylla
<400> 27
gaagggattg atgaaacaaa taaaaaaaaa attcaattta tttcgactat aaaaaagtat 60
ctttctaata ttagtaataa taaatcaaaa atttctggtg acccatattc cttttcacaa 120
gcatctgtat tttacaaatt atcacaaatc gaagctatca agaagtatca tttgagatct 180
ctacttcaat atcgcgaagc atatcttatt cttaaggata gaatcaggaa tttttttgga 240
acacgaagaa tatttgattc caaatcaagg cataaaaaac ttccgaattc tggaatgaat 300
gaatggaaaa actggttaag gggtcattat caatacaatt tatctcagga tttatcgata 360
atattttttg ttgattcaag gaaaagttgt acattgactc tagaaatatt aaccgtacat 420
agaaagatat gtatgtatat tctttcgttg aaaaatgtca aaaaatagtg ccatttgcgt 480
atgaatatga atctgttact tcttcttttt gatatctgcc aaataggttt ctgcgattcc 540
gatcttttat caccacaact tgtatcgtta ggactaatat ttatatccgg agttagaaat 600
atttttcttt tgtcttttgc acccctttct atttgatttc tgattagggt tgttctatcg 660
ttttcctcct cccgctcttc aaaagaatcg atcaatttgt atgatcgtcg aggaatcttt 720
ttaccgattt cttctattcc aatagatttt ttttgaattg tttgattatt tggatcagtt 780
gtaattacat cgaatagaaa tttcaaacat ttttttttat tttctgaatc aaatcttctt 840
tgttcggata ttaaaacaag ctttttcaaa ttaagattaa aaccagttgt tgatttccta 900
gctaattcac tgatagaggt caagaaagga ccaatgtctg tcgataatga ttctccatta 960
aatatatcca ttttatgttc aagtttttgg taatcagtag gaagcatatc atgaatctta 1020
tttatccaaa ccattcctat ggaatcttct gtccccattc cacctaaaat cctaggaatt 1080
cgttgatggt tggaatagat tcgtagaccg ggacgactga tacgctttaa aatagttcta 1140
tatgttcctt tcctattcct tctatgtcgc agggttgaaa ccaaaaaata tttgttgttt 1200
tccctatgtt tcctaacatt ttcaataaac ccttcttgta gaagtatttt aacaatgttt 1260
tcggcgatat tagtagatgc tattcgaacc gttccttttt tatccatgtt agcatttctt 1320
atagaagtta ttatatcggc aatagtgtcc ctacccatga cgaactaaaa ttatgggtgc 1380
cttccagttt tgatacaatc aacatgttcc tttttttttt tcatctacta gtatttataa 1440
gacttcagga gctaatgaga ctattttagt gaaattcaac tgtctcaatt cccgcgcgat 1500
cgctccaaaa acgcgagttc cttttggatt tccttcttga tcaatgacaa ctgctgcatt 1560
gtcatcatat cgtattatca taccgttgtc gcgtttgagt tctttacatg tacgtacaat 1620
tacagctctg atcacttctg atctttcgag aggcatattg ggcactgctt ctttgattac 1680
agcaacaata acgtcaccaa tatgagcata tcgttgatta ctagctccta tgattcgaat 1740
acacatcaat tctcgagccc cgctgttgtc cgctacattc aaatgggtct gaggttgaat 1800
catatcattt tttttttaac aaagatcaaa gtgaaattca aaaaaaaact gggtgggcca 1860
ttcagcgtat gttcgccccc tcgcccatat tcatattgaa ggttagttag tcatttggat 1920
aaactttatg tcccatatct atgatatttg gattctcaca tgatgcattc tgagtcgaaa 1980
tgcgaaataa aagagaagtc tcgaccttcc ctaaagtaaa tataaataaa gatagggtag 2040
acaaaatgac taattctatg tggatcctga atcctaaaaa acgggggggt tcttggtatt 2100
aattccagcg atggaattaa agctcctgag actggggtgg gacaaaatca aacaaaatag 2160
taacaacgaa ttgatatgaa cccattttgc tttgtactta tacaagacag gatagcatcc 2220
cataagaaga tccttttaaa ttggctttgg atatgattca tgatccccag ggcagaatta 2280
agacagatca aactaaagat attaagcata acaaacattt tgttcttgga gaaaatttca 2340
ttattattgg gttattgata taaggggaaa atgaagaaag aagggaaagt aggccgcaaa 2400
atatttcttt ttctttgaac tccctcaatc aaaaatcctt caattctcaa atgagaatag 2460
aaggaatcat accttacata caatatctta attgaattat atgtaatgat caataaattc 2520
attttgattc tacatctaca tgtgtaaaat catagcaaca accaattcaa tagatattgg 2580
ggctggaatt gacgaacaac caataccgat attagtgttt atcggtgtcg aatagaaata 2640
aaaatggggc gtggccaagt ggtaaggcaa cgggttttgg tcccgttact cggaggt 2697
<210> 28
<211> 2698
<212> DNA
<213> Machilus pauhoi
<400> 28
gaagggattg atgaaacaaa tcaaaaaaaa attcaattta tttcgactat aaaaaagtct 60
ctttctaata ttagtaataa taaatcaaaa atttctggtg acccatattc cttttcacaa 120
gcatctgtat tttacaaatt atcacaaatc gaagctatca agaagtatca tttgagatct 180
ctacttcaat atcgcgaagc atatcttatt cttaaggata gaatcaggaa tttttttgga 240
acacgaagaa tatttgattc caaatcaagg cataaaaaac ttccgaattc tggaatgaat 300
gaatggaaaa actggttaag gggtcattat caatacaatt tatctcagga tttatcgata 360
atcttttttg ttgattcaag gaaaagttgt acattgactc tagaaatatt aaccgtacat 420
agaaagatat gtatgtatat tctttcgttg aaaaatgtca aaaaatagtg ccatttgcgt 480
atgaatatga atctgttact tcttcttttt gatatctgcc aaataggttt ctgcgattcc 540
gatcttttat caccacaact tgtatcgtta ggactaatat ttatatccgg agttagaaat 600
atttttcttt tgtcttttgc acccctttct atttgatttc tgattagggt tgttctatcg 660
ttttcctcct cccgctcttc aaaagaatcg atcaatttgt atgatcgtcg aggaatcttt 720
ttaccgattt cttctattcc aatagatttt ttttgaattg tttgattatt tggatcagtt 780
gtaattacat cgaatagaaa tttcaaacat tttgttttat tttctgaatc aaatcttctt 840
tgttcggata ttaaaacaag ctttttcaaa ttaagattaa aaccagttgt tgatttccta 900
gctaattcac tgatagaggt caagaaagga ccaatgtctg tcgataatga ttctccatta 960
aatatatcca ttttatgttc aagtttttgg taatcagtag gaagcatatc atgaatctta 1020
tttatccaaa ccattcctat ggaatcttct gtccccattc cacctaaaat cctaggaatt 1080
cgttgatggt tggaatagat tcgtagaccg ggacgactga tacgctttaa aatagttcta 1140
tatgttcctt tcctattcct tctatgtcgc agggttgaaa ccaaaaaata tttgttgttt 1200
tccctatgtt tcctaacatt ttcaataaac ccttcttgta gaagtatttt aacaatgttt 1260
tcggcgatat tagtagatgc tattcgaacc gttccttttt tatccatgtt agcatttctt 1320
atagaagtta ttatatcggc aatagtgtcc ctacccatga cgaactaaaa ttatgggtgc 1380
cttccagttt tgatacaatc aacatgttcc tttttttttt tcatctacta gtatttataa 1440
gacttcagga gctaatgaga ctattttagt gaaattcaac tgtctcaatt cccgcgcgat 1500
cgctccaaaa acgcgagttc cttttggatt tccttcttga tcaatgacaa ctgctgcatt 1560
gtcatcatat cgtattatca taccgttgtc gcgtttgagt tctttacatg tacgtacaat 1620
tacagctctg atcacttctg atctttcgag aggcatattg ggcactgctt ctttgattac 1680
agcaacaata acgtcaccaa tatgagcata tcgttgatta ctagctccta tgattcgaat 1740
acacatcaat tctcgagccc cgctgttgtc cgctacattc aaatgggtct gaggttgaat 1800
catatcattt tttttttaac aaagatcaaa gtgaaattca aaaaaaaaac tgggtgggcc 1860
attcagcgta tgttcgcccc ctcgcccata ttcatattga aggttagtta gtcatttgga 1920
taaactttat gtcccatatc tatgatattt ggattctcac atgatgcatt ctgagtcgaa 1980
atgcgaaata aaagagaagt ctcgaccttc cctaaagtaa atataaataa agatagggta 2040
gacaaaatga ctaattctat gtggatcctg aatcctaaaa aacggggggg ttcttggtat 2100
taattccagc gatggaatta aagctcctga gactggggtg ggacaaaatc aaacaaaata 2160
gtaacaacga attgatatga acccattttg ctttgtactt atacaagaca ggatagcatc 2220
ccataagaag atccttttaa attggctttg gatatgattc atgatcccca gggcagaatt 2280
aagacagatc aaactaaaga tattaagcat aacaaacatt ttgttcttgg agaaaatttc 2340
attattattg ggttattgat ataaggggaa aatgaagaaa gaagggaaag taggccgcaa 2400
aatatttctt tttctttgaa ctccctcaat caaaaatcct tcaattctca aatgagaata 2460
gaaggaatca taccttacat acaatatctt aattgaatta tatgtaatga tcaataaatt 2520
cattttgatt ctacatctac atgtgtaaaa tcatagcaac aaccaattca atagatattg 2580
gggctggaat tgacgaacaa ccaataccga tattagtgtt tatcggtgtc gaatagaaat 2640
aaaaatgggg cgtggccaag tggtaaggca acgggttttg gtcccgttac tcggaggt 2698
<210> 29
<211> 2698
<212> DNA
<213> Machilus pauhoi
<400> 29
gaagggattg atgaaacaaa tcaaaaaaaa attcaattta tttcgactat aaaaaagtct 60
ctttctaata ttagtaataa taaatcaaaa atttctggtg acccatattc cttttcacaa 120
gcatctgtat tttacaaatt atcacaaatc gaagctatca agaagtatca tttgagatct 180
ctacttcaat atcgcgaagc atatcttatt cttaaggata gaatcaggaa tttttttgga 240
acacgaagaa tatttgattc caaatcaagg cataaaaaac ttccgaattc tggaatgaat 300
gaatggaaaa actggttaag gggtcattat caatacaatt tatctcagga tttatcgata 360
atcttttttg ttgattcaag gaaaagttgt acattgactc tagaaatatt aaccgtacat 420
agaaagatat gtatgtatat tctttcgttg aaaaatgtca aaaaatagtg ccatttgcgt 480
atgaatatga atctgttact tcttcttttt gatatctgcc aaataggttt ctgcgattcc 540
gatcttttat caccacaact tgtatcgtta ggactaatat ttatatccgg agttagaaat 600
atttttcttt tgtcttttgc acccctttct atttgatttc tgattagggt tgttctatcg 660
ttttcctcct cccgctcttc aaaagaatcg atcaatttgt atgatcgtcg aggaatcttt 720
ttaccgattt cttctattcc aatagatttt ttttgaattg tttgattatt tggatcagtt 780
gtaattacat cgaatagaaa tttcaaacat tttgttttat tttctgaatc aaatcttctt 840
tgttcggata ttaaaacaag ctttttcaaa ttaagattaa aaccagttgt tgatttccta 900
gctaattcac tgatagaggt caagaaagga ccaatgtctg tcgataatga ttctccatta 960
aatatatcca ttttatgttc aagtttttgg taatcagtag gaagcatatc atgaatctta 1020
tttatccaaa ccattcctat ggaatcttct gtccccattc cacctaaaat cctaggaatt 1080
cgttgatggt tggaatagat tcgtagaccg ggacgactga tacgctttaa aatagttcta 1140
tatgttcctt tcctattcct tctatgtcgc agggttgaaa ccaaaaaata tttgttgttt 1200
tccctatgtt tcctaacatt ttcaataaac ccttcttgta gaagtatttt aacaatgttt 1260
tcggcgatat tagtagatgc tattcgaacc gttccttttt tatccatgtt agcatttctt 1320
atagaagtta ttatatcggc aatagtgtcc ctacccatga cgaactaaaa ttatgggtgc 1380
cttccagttt tgatacaatc aacatgttcc tttttttttt tcatctacta gtatttataa 1440
gacttcagga gctaatgaga ctattttagt gaaattcaac tgtctcaatt cccgcgcgat 1500
cgctccaaaa acgcgagttc cttttggatt tccttcttga tcaatgacaa ctgctgcatt 1560
gtcatcatat cgtattatca taccgttgtc gcgtttgagt tctttacatg tacgtacaat 1620
tacagctctg atcacttctg atctttcgag aggcatattg ggcactgctt ctttgattac 1680
agcaacaata acgtcaccaa tatgagcata tcgttgatta ctagctccta tgattcgaat 1740
acacatcaat tctcgagccc cgctgttgtc cgctacattc aaatgggtct gaggttgaat 1800
catatcattt tttttttaac aaagatcaaa gtgaaattca aaaaaaaaac tgggtgggcc 1860
attcagcgta tgttcgcccc ctcgcccata ttcatattga aggttagtta gtcatttgga 1920
taaactttat gtcccatatc tatgatattt ggattctcac atgatgcatt ctgagtcgaa 1980
atgcgaaata aaagagaagt ctcgaccttc cctaaagtaa atataaataa agatagggta 2040
gacaaaatga ctaattctat gtggatcctg aatcctaaaa aacggggggg ttcttggtat 2100
taattccagc gatggaatta aagctcctga gactggggtg ggacaaaatc aaacaaaata 2160
gtaacaacga attgatatga acccattttg ctttgtactt atacaagaca ggatagcatc 2220
ccataagaag atccttttaa attggctttg gatatgattc atgatcccca gggcagaatt 2280
aagacagatc aaactaaaga tattaagcat aacaaacatt ttgttcttgg agaaaatttc 2340
attattattg ggttattgat ataaggggaa aatgaagaaa gaagggaaag taggccgcaa 2400
aatatttctt tttctttgaa ctccctcaat caaaaatcct tcaattctca aatgagaata 2460
gaaggaatca taccttacat acaatatctt aattgaatta tatgtaatga tcaataaatt 2520
cattttgatt ctacatctac atgtgtaaaa tcatagcaac aaccaattca atagatattg 2580
gggctggaat tgacgaacaa ccaataccga tattagtgtt tatcggtgtc gaatagaaat 2640
aaaaatgggg cgtggccaag tggtaaggca acgggttttg gtcccgttac tcggaggt 2698
<210> 30
<211> 2698
<212> DNA
<213> Machilus pauhoi
<400> 30
gaagggattg atgaaacaaa tcaaaaaaaa attcaattta tttcgactat aaaaaagtct 60
ctttctaata ttagtaataa taaatcaaaa atttctggtg acccatattc cttttcacaa 120
gcatctgtat tttacaaatt atcacaaatc gaagctatca agaagtatca tttgagatct 180
ctacttcaat atcgcgaagc atatcttatt cttaaggata gaatcaggaa tttttttgga 240
acacgaagaa tatttgattc caaatcaagg cataaaaaac ttccgaattc tggaatgaat 300
gaatggaaaa actggttaag gggtcattat caatacaatt tatctcagga tttatcgata 360
atcttttttg ttgattcaag gaaaagttgt acattgactc tagaaatatt aaccgtacat 420
agaaagatat gtatgtatat tctttcgttg aaaaatgtca aaaaatagtg ccatttgcgt 480
atgaatatga atctgttact tcttcttttt gatatctgcc aaataggttt ctgcgattcc 540
gatcttttat caccacaact tgtatcgtta ggactaatat ttatatccgg agttagaaat 600
atttttcttt tgtcttttgc acccctttct atttgatttc tgattagggt tgttctatcg 660
ttttcctcct cccgctcttc aaaagaatcg atcaatttgt atgatcgtcg aggaatcttt 720
ttaccgattt cttctattcc aatagatttt ttttgaattg tttgattatt tggatcagtt 780
gtaattacat cgaatagaaa tttcaaacat tttgttttat tttctgaatc aaatcttctt 840
tgttcggata ttaaaacaag ctttttcaaa ttaagattaa aaccagttgt tgatttccta 900
gctaattcac tgatagaggt caagaaagga ccaatgtctg tcgataatga ttctccatta 960
aatatatcca ttttatgttc aagtttttgg taatcagtag gaagcatatc atgaatctta 1020
tttatccaaa ccattcctat ggaatcttct gtccccattc cacctaaaat cctaggaatt 1080
cgttgatggt tggaatagat tcgtagaccg ggacgactga tacgctttaa aatagttcta 1140
tatgttcctt tcctattcct tctatgtcgc agggttgaaa ccaaaaaata tttgttgttt 1200
tccctatgtt tcctaacatt ttcaataaac ccttcttgta gaagtatttt aacaatgttt 1260
tcggcgatat tagtagatgc tattcgaacc gttccttttt tatccatgtt agcatttctt 1320
atagaagtta ttatatcggc aatagtgtcc ctacccatga cgaactaaaa ttatgggtgc 1380
cttccagttt tgatacaatc aacatgttcc tttttttttt tcatctacta gtatttataa 1440
gacttcagga gctaatgaga ctattttagt gaaattcaac tgtctcaatt cccgcgcgat 1500
cgctccaaaa acgcgagttc cttttggatt tccttcttga tcaatgacaa ctgctgcatt 1560
gtcatcatat cgtattatca taccgttgtc gcgtttgagt tctttacatg tacgtacaat 1620
tacagctctg atcacttctg atctttcgag aggcatattg ggcactgctt ctttgattac 1680
agcaacaata acgtcaccaa tatgagcata tcgttgatta ctagctccta tgattcgaat 1740
acacatcaat tctcgagccc cgctgttgtc cgctacattc aaatgggtct gaggttgaat 1800
catatcattt tttttttaac aaagatcaaa gtgaaattca aaaaaaaaac tgggtgggcc 1860
attcagcgta tgttcgcccc ctcgcccata ttcatattga aggttagtta gtcatttgga 1920
taaactttat gtcccatatc tatgatattt ggattctcac atgatgcatt ctgagtcgaa 1980
atgcgaaata aaagagaagt ctcgaccttc cctaaagtaa atataaataa agatagggta 2040
gacaaaatga ctaattctat gtggatcctg aatcctaaaa aacggggggg ttcttggtat 2100
taattccagc gatggaatta aagctcctga gactggggtg ggacaaaatc aaacaaaata 2160
gtaacaacga attgatatga acccattttg ctttgtactt atacaagaca ggatagcatc 2220
ccataagaag atccttttaa attggctttg gatatgattc atgatcccca gggcagaatt 2280
aagacagatc aaactaaaga tattaagcat aacaaacatt ttgttcttgg agaaaatttc 2340
attattattg ggttattgat ataaggggaa aatgaagaaa gaagggaaag taggccgcaa 2400
aatatttctt tttctttgaa ctccctcaat caaaaatcct tcaattctca aatgagaata 2460
gaaggaatca taccttacat acaatatctt aattgaatta tatgtaatga tcaataaatt 2520
cattttgatt ctacatctac atgtgtaaaa tcatagcaac aaccaattca atagatattg 2580
gggctggaat tgacgaacaa ccaataccga tattagtgtt tatcggtgtc gaatagaaat 2640
aaaaatgggg cgtggccaag tggtaaggca acgggttttg gtcccgttac tcggaggt 2698
<210> 31
<211> 2698
<212> DNA
<213> Machilus pauhoi
<400> 31
gaagggattg atgaaacaaa tcaaaaaaaa attcaattta tttcgactat aaaaaagtct 60
ctttctaata ttagtaataa taaatcaaaa atttctggtg acccatattc cttttcacaa 120
gcatctgtat tttacaaatt atcacaaatc gaagctatca agaagtatca tttgagatct 180
ctacttcaat atcgcgaagc atatcttatt cttaaggata gaatcaggaa tttttttgga 240
acacgaagaa tatttgattc caaatcaagg cataaaaaac ttccgaattc tggaatgaat 300
gaatggaaaa actggttaag gggtcattat caatacaatt tatctcagga tttatcgata 360
atcttttttg ttgattcaag gaaaagttgt acattgactc tagaaatatt aaccgtacat 420
agaaagatat gtatgtatat tctttcgttg aaaaatgtca aaaaatagtg ccatttgcgt 480
atgaatatga atctgttact tcttcttttt gatatctgcc aaataggttt ctgcgattcc 540
gatcttttat caccacaact tgtatcgtta ggactaatat ttatatccgg agttagaaat 600
atttttcttt tgtcttttgc acccctttct atttgatttc tgattagggt tgttctatcg 660
ttttcctcct cccgctcttc aaaagaatcg atcaatttgt atgatcgtcg aggaatcttt 720
ttaccgattt cttctattcc aatagatttt ttttgaattg tttgattatt tggatcagtt 780
gtaattacat cgaatagaaa tttcaaacat tttgttttat tttctgaatc aaatcttctt 840
tgttcggata ttaaaacaag ctttttcaaa ttaagattaa aaccagttgt tgatttccta 900
gctaattcac tgatagaggt caagaaagga ccaatgtctg tcgataatga ttctccatta 960
aatatatcca ttttatgttc aagtttttgg taatcagtag gaagcatatc atgaatctta 1020
tttatccaaa ccattcctat ggaatcttct gtccccattc cacctaaaat cctaggaatt 1080
cgttgatggt tggaatagat tcgtagaccg ggacgactga tacgctttaa aatagttcta 1140
tatgttcctt tcctattcct tctatgtcgc agggttgaaa ccaaaaaata tttgttgttt 1200
tccctatgtt tcctaacatt ttcaataaac ccttcttgta gaagtatttt aacaatgttt 1260
tcggcgatat tagtagatgc tattcgaacc gttccttttt tatccatgtt agcatttctt 1320
atagaagtta ttatatcggc aatagtgtcc ctacccatga cgaactaaaa ttatgggtgc 1380
cttccagttt tgatacaatc aacatgttcc tttttttttt tcatctacta gtatttataa 1440
gacttcagga gctaatgaga ctattttagt gaaattcaac tgtctcaatt cccgcgcgat 1500
cgctccaaaa acgcgagttc cttttggatt tccttcttga tcaatgacaa ctgctgcatt 1560
gtcatcatat cgtattatca taccgttgtc gcgtttgagt tctttacatg tacgtacaat 1620
tacagctctg atcacttctg atctttcgag aggcatattg ggcactgctt ctttgattac 1680
agcaacaata acgtcaccaa tatgagcata tcgttgatta ctagctccta tgattcgaat 1740
acacatcaat tctcgagccc cgctgttgtc cgctacattc aaatgggtct gaggttgaat 1800
catatcattt tttttttaac aaagatcaaa gtgaaattca aaaaaaaaac tgggtgggcc 1860
attcagcgta tgttcgcccc ctcgcccata ttcatattga aggttagtta gtcatttgga 1920
taaactttat gtcccatatc tatgatattt ggattctcac atgatgcatt ctgagtcgaa 1980
atgcgaaata aaagagaagt ctcgaccttc cctaaagtaa atataaataa agatagggta 2040
gacaaaatga ctaattctat gtggatcctg aatcctaaaa aacggggggg ttcttggtat 2100
taattccagc gatggaatta aagctcctga gactggggtg ggacaaaatc aaacaaaata 2160
gtaacaacga attgatatga acccattttg ctttgtactt atacaagaca ggatagcatc 2220
ccataagaag atccttttaa attggctttg gatatgattc atgatcccca gggcagaatt 2280
aagacagatc aaactaaaga tattaagcat aacaaacatt ttgttcttgg agaaaatttc 2340
attattattg ggttattgat ataaggggaa aatgaagaaa gaagggaaag taggccgcaa 2400
aatatttctt tttctttgaa ctccctcaat caaaaatcct tcaattctca aatgagaata 2460
gaaggaatca taccttacat acaatatctt aattgaatta tatgtaatga tcaataaatt 2520
cattttgatt ctacatctac atgtgtaaaa tcatagcaac aaccaattca atagatattg 2580
gggctggaat tgacgaacaa ccaataccga tattagtgtt tatcggtgtc gaatagaaat 2640
aaaaatgggg cgtggccaag tggtaaggca acgggttttg gtcccgttac tcggaggt 2698
<210> 32
<211> 2698
<212> DNA
<213> Machilus pauhoi
<400> 32
gaagggattg atgaaacaaa tcaaaaaaaa attcaattta tttcgactat aaaaaagtct 60
ctttctaata ttagtaataa taaatcaaaa atttctggtg acccatattc cttttcacaa 120
gcatctgtat tttacaaatt atcacaaatc gaagctatca agaagtatca tttgagatct 180
ctacttcaat atcgcgaagc atatcttatt cttaaggata gaatcaggaa tttttttgga 240
acacgaagaa tatttgattc caaatcaagg cataaaaaac ttccgaattc tggaatgaat 300
gaatggaaaa actggttaag gggtcattat caatacaatt tatctcagga tttatcgata 360
atcttttttg ttgattcaag gaaaagttgt acattgactc tagaaatatt aaccgtacat 420
agaaagatat gtatgtatat tctttcgttg aaaaatgtca aaaaatagtg ccatttgcgt 480
atgaatatga atctgttact tcttcttttt gatatctgcc aaataggttt ctgcgattcc 540
gatcttttat caccacaact tgtatcgtta ggactaatat ttatatccgg agttagaaat 600
atttttcttt tgtcttttgc acccctttct atttgatttc tgattagggt tgttctatcg 660
ttttcctcct cccgctcttc aaaagaatcg atcaatttgt atgatcgtcg aggaatcttt 720
ttaccgattt cttctattcc aatagatttt ttttgaattg tttgattatt tggatcagtt 780
gtaattacat cgaatagaaa tttcaaacat tttgttttat tttctgaatc aaatcttctt 840
tgttcggata ttaaaacaag ctttttcaaa ttaagattaa aaccagttgt tgatttccta 900
gctaattcac tgatagaggt caagaaagga ccaatgtctg tcgataatga ttctccatta 960
aatatatcca ttttatgttc aagtttttgg taatcagtag gaagcatatc atgaatctta 1020
tttatccaaa ccattcctat ggaatcttct gtccccattc cacctaaaat cctaggaatt 1080
cgttgatggt tggaatagat tcgtagaccg ggacgactga tacgctttaa aatagttcta 1140
tatgttcctt tcctattcct tctatgtcgc agggttgaaa ccaaaaaata tttgttgttt 1200
tccctatgtt tcctaacatt ttcaataaac ccttcttgta gaagtatttt aacaatgttt 1260
tcggcgatat tagtagatgc tattcgaacc gttccttttt tatccatgtt agcatttctt 1320
atagaagtta ttatatcggc aatagtgtcc ctacccatga cgaactaaaa ttatgggtgc 1380
cttccagttt tgatacaatc aacatgttcc tttttttttt tcatctacta gtatttataa 1440
gacttcagga gctaatgaga ctattttagt gaaattcaac tgtctcaatt cccgcgcgat 1500
cgctccaaaa acgcgagttc cttttggatt tccttcttga tcaatgacaa ctgctgcatt 1560
gtcatcatat cgtattatca taccgttgtc gcgtttgagt tctttacatg tacgtacaat 1620
tacagctctg atcacttctg atctttcgag aggcatattg ggcactgctt ctttgattac 1680
agcaacaata acgtcaccaa tatgagcata tcgttgatta ctagctccta tgattcgaat 1740
acacatcaat tctcgagccc cgctgttgtc cgctacattc aaatgggtct gaggttgaat 1800
catatcattt tttttttaac aaagatcaaa gtgaaattca aaaaaaaaac tgggtgggcc 1860
attcagcgta tgttcgcccc ctcgcccata ttcatattga aggttagtta gtcatttgga 1920
taaactttat gtcccatatc tatgatattt ggattctcac atgatgcatt ctgagtcgaa 1980
atgcgaaata aaagagaagt ctcgaccttc cctaaagtaa atataaataa agatagggta 2040
gacaaaatga ctaattctat gtggatcctg aatcctaaaa aacggggggg ttcttggtat 2100
taattccagc gatggaatta aagctcctga gactggggtg ggacaaaatc aaacaaaata 2160
gtaacaacga attgatatga acccattttg ctttgtactt atacaagaca ggatagcatc 2220
ccataagaag atccttttaa attggctttg gatatgattc atgatcccca gggcagaatt 2280
aagacagatc aaactaaaga tattaagcat aacaaacatt ttgttcttgg agaaaatttc 2340
attattattg ggttattgat ataaggggaa aatgaagaaa gaagggaaag taggccgcaa 2400
aatatttctt tttctttgaa ctccctcaat caaaaatcct tcaattctca aatgagaata 2460
gaaggaatca taccttacat acaatatctt aattgaatta tatgtaatga tcaataaatt 2520
cattttgatt ctacatctac atgtgtaaaa tcatagcaac aaccaattca atagatattg 2580
gggctggaat tgacgaacaa ccaataccga tattagtgtt tatcggtgtc gaatagaaat 2640
aaaaatgggg cgtggccaag tggtaaggca acgggttttg gtcccgttac tcggaggt 2698
<210> 33
<211> 2700
<212> DNA
<213> Phoebe zhennan
<400> 33
gaagggattg atgaaacaaa tcaaaaaaaa attcacttta tttcgactat aaaaaagtct 60
ctttctaata ttagtaataa taaatcaaag atttctggtg acctatattc cttttcacaa 120
gcatctgtat tttacaaatt atcacaaatc caagctatta agaagtatca tttgagatct 180
ctacttcaat atcgcgaagc atatcttatt cttaaggaaa gaatcaggaa tttttttgga 240
acacgaagaa tatttgattc caaatcaagg cataaaaaac ttcagaattc tggaatgaat 300
gaatggaaaa actggttaag gggtcattat caatacaatt tatctcagga tttatcgata 360
atcttttttg ttgattcaag gaaaagttgt acattgactc tagaaatatt aacagtactt 420
agaaagatat gtatgtatat tctttcgttg aaaaatgtca aaaaatagtg ccatttgcgt 480
atgaatatga atctgttact tcttcttttt gatatctgcc aaatgggttt ctgcgattcc 540
gatcttttat caccacaact tgtatcgtta ggactaatat ttatatccgg agttagaaat 600
atttttcttt tgtcttttgc acccctttct atttgatttc tgattagggt tgttctatcg 660
ttttcctcct cccgctcttc aaaagaatcg atcaatttgt atgatcgtcg aggaatcttt 720
ttaccgattt cttctattcc aatagatttt ttttgaattg tttgattatt tggatcagtt 780
gtaattacat cgaatagaaa tttcaaacat tttgttttat tttctgaatc aaatcttctt 840
tgttcggata ttaaaacaag ctttttaaaa ttaagattaa aaccagttgt tgatttccta 900
gctaattcac tgatagaggt caagaaagga ccaatgtctg tcgataatga ttctccatta 960
aatatatcca ttttatgttc aagtttttgg taatcagtag gaagcatatc atgaatctta 1020
tttatccaaa ccattcctat agaatcttct gtccccattc cacctaaaat cctaggaatt 1080
cgttgatggt tggaatagat tcgtagaccg ggacgactga tacgctttaa aatatttcta 1140
tatgttcctt tcctattcct tctatgtcgc agggttgaaa ccaaaaaata tttgttgttt 1200
tccctatgtt tcctaacatt ttcaataaac ccttcttgta gaagtatttt aacaatgttt 1260
tcggcgatat tagtagatgc tattcgaacc gttccttttt tatccatgtt agcatttctt 1320
atagaagtta ttatatcggc aatagtgtcc ctacccatga cgaactaaaa ttatgggtgc 1380
cttccagttt tgatataatc aacatgttac tttttttttt tcatctacta gtatttataa 1440
gacttcagga gctaatgaga ctattttagt gaaattcaac tgtctcaatt cccgcgcgat 1500
cgctccaaaa actcgagttc cttttggatt tccttcttga tcaatgacaa ctgctgcatt 1560
gtcatcatat cgtattatca taccgttgtc gcgcttgagt tctttacatg tacgtacaat 1620
tacagctctg atcacttctg atctttcgag aggcatattg ggcactgctt ctttgattac 1680
agcaacaata acgtcaccaa tatgagcata tcgttgatta ctagctccta tgattcgaat 1740
acacatcaat tctcgagccc cgctgttgtc cgctacattc aaatgggtct gaggttgaat 1800
catatcattt tttttttaac aaagatcaaa gtgaaattca aaaaaaaaaa actgggtggg 1860
ccattcagcg tatgttcgcc ccctcgccca tattcatatt gaaggttagt tagtcatttg 1920
gataaacttt atgccccata tctatgatat ttggattctc acatgatgca ttctgagtcg 1980
aaatgcgaaa taaaagagaa gtctctacct tccctaaagt aaatataaat aaagataggg 2040
tagacaaaat gactaattct atgtggatcc tgaatcctaa aaaacagggg ggttcttggt 2100
attaattcca tcgctggaat taaagctcct gagactgggg tgggacaaaa tcaaacaaaa 2160
tagtaacaac gaattgatat gaacccattt tgctttgtac ttatacaaga caggatagca 2220
tcccataaga agatcctttt aaattggctt tggatatcat tcatgatccc cagggcagaa 2280
ttaagacaga tcaaactaaa gatattaagc ataacaaaca ttttgttctt ggagaaaatt 2340
tcattattat tgggttattg atataagggg aaaatgaaga aagaagggaa agtaggccgc 2400
aaaatctttc tttttctttg aactccctca atcaaaaatc cttcaattct caaatgagaa 2460
tagaaggaat cataccttac atacaatatc ttaattgaat tatatgtaat gatcaataaa 2520
ttcattttga ttctacatct acatgtgtag aatcatagca acaaccaatt caatagatat 2580
tggggctgga attgacgaac aaccaatacc gatattagtg tttatcggtg tcgaatagaa 2640
ataaaaatgg ggcgtggcca agtggtaagg caacgggttt tggtcccgtt actcggaggt 2700
<210> 34
<211> 2699
<212> DNA
<213> Phoebe bournei
<400> 34
gaagggattg atgaaacaaa tcaaaaaaaa attcacttta tttcgactat aaaaaagtct 60
ctttctaata ttagtaataa taaatcaaag atttctggtg acctatattc cttttcacaa 120
gcatctgtat tttacaaatt atcacaaatc caagctatta agaagtatca tttgagatct 180
ctacttcaat atcgcgaagc atatcttatt cttaaggaaa gaatcaggaa tttttttgga 240
acacgaagaa tatttgattc caaatcaagg cataaaaaac ttcagaattc tggaatgaat 300
gaatggaaaa actggttaag gggtcattat caatacaatt tatctcagga tttatcgata 360
atcttttttg ttgattcaag gaaaagttgt acattgactc tagaaatatt aacagtactt 420
agaaagatat gtatgtatat tctttcgttg aaaaatgtca aaaaatagtg ccatttgcgt 480
atgaatatga atctgttact tcttcttttt gatatctgcc aaatgggttt ctgcgattcc 540
gatcttttat caccacaact tgtatcgtta ggactaatat ttatatccgg agttagaaat 600
atttttcttt tgtcttttgc acccctttct atttgatttc tgattagggt tgttctatcg 660
ttttcctcct cccgctcttc aaaagaatcg atcaatttgt atgatcgtcg aggaatcttt 720
ttaccgattt cttctattcc aatagatttt ttttgaattg tttgattatt tggatcagtt 780
gtaattacat cgaatagaaa tttcaaacat ttttttttat tttctgaatc aaatcttctt 840
tgttcggata ttaaaacaag ctttttaaaa ttaagattaa aaccagttgt tgatttccta 900
gctaattcac tgatagaggt caagaaagga ccaatgtctg tcgataatga ttctccatta 960
aatatatcca ttttatgctc aagtttttgg taatcagtag gaagcatatc atgaatctta 1020
tttatccaaa ccattcctat agaatcttct gtccccattc cacctaaaat cctaggaatt 1080
cgttgatggt tggaatagat tcgtagaccg ggacgactga tacgctttaa aatatttcta 1140
tatgttcctt tcctattcct tctatgtcgc agggttgaaa ccaaaaaata tttgttgttt 1200
tccctatgtt tcctaacatt ttcaataaac ccttcttgta gaagtatttt aacaatgttt 1260
tcggcgatat tagtagatgc tattcgaacc gttccttttt tatccatgtt agcatttctt 1320
atagaagtta ttatatcggc aatagtgtcc ctacccatga cgaactaaaa ttatgggtgc 1380
cttccagttt tgatataatc aacatgttac tttttttttt tcatctacta gtatttataa 1440
gacttcagga gctaatgaga ctattttagt gaaattcaac tgtctcaatt cccgcgcgat 1500
cgctccaaaa actcgagttc cttttggatt tccttcttga tcaatgacaa ctgctgcatt 1560
gtcatcatat cgtattatca taccgttgtc gcgcttgagt tctttacatg tacgtacaat 1620
tacagctctg atcacttctg atctttcgag aggcatattg ggcactgctt ctttgattac 1680
agcaacaata acgtcaccaa tatgagcata tcgttgatta ctagctccta tgattcgaat 1740
acacatcaat tctcgagccc cgctgttgtc cgctacattc aaatgggtct gaggttgaat 1800
catatcattt tttttttaac aaagatcaaa gtgaaattca aaaaaaaaaa ctgggtgggc 1860
cattcagcgt atgttcgccc cctcgcccat attcatattg aaggttagtt agtcatttgg 1920
ataaacttta tgccccatat ctatgatatt tggattctca catgatgcat tctgagtcga 1980
aatgcgaaat aaaagagaag tctctacctt ccctaaagta aatataaata aagatagggt 2040
agacaaaatg actaattcta tgtggatcct gaatcctaaa aaacaggggg gttcttggta 2100
ttaattccat cgctggaatt aaagctcctg agactggggt gggacaaaat caaacaaaat 2160
agtaacaacg aattgatatg aacccatttt gctttgtact tatacaagac aggatagcat 2220
cccataagaa gatcctttta aattggcttt ggatatgatt catgatcccc agggcagaat 2280
taagacagat caaactaaag atattaagca taacaaacat tttgttcttg gagaaaattt 2340
cattattatt gggttattga tataagggga aaatgaagaa agaagggaaa gtaggccgca 2400
aaatctttct ttttctttga actccctcaa tcaaaaatcc ttcaattctc aaatgagaat 2460
agaaggaatc ataccttaca tacaatatct taattgaatt atatgtaatg atcaataaat 2520
tcattttgat tctacatcta catgtgtaga atcatagcaa caaccaattc aatagatatt 2580
ggggctggaa ttgacgaaca accaataccg atattagtgt ttatcggtgt cgaatagaaa 2640
taaaaatggg gcgtggccaa gtggtaaggc aacgggtttt ggtcccgtta ctcggaggt 2699
<210> 35
<211> 2699
<212> DNA
<213> Phoebe chekiangensis
<400> 35
gaagggattg atgaaacaaa tcaaaaaaaa attcacttta tttcgactat aaaaaagtct 60
ctttctaata ttagtaataa taaatcaaag atttctggtg acctatattc cttttcacaa 120
gcatctgtat tttacaaatt atcacaaatc caagctatta agaagtatca tttgagatct 180
ctacttcaat atcgcgaagc atatcttatt cttaaggaaa gaatcaggaa tttttttgga 240
acacgaagaa tatttgattc caaatcaagg cataaaaaac ttcagaattc tggaatgaat 300
gaatggaaaa actggttaag gggtcattat caatacaatt tatctcagga tttatcgata 360
atcttttttg ttgattcaag gaaaagttgt acattgactc tagaaatatt aacagtactt 420
agaaagatat gtatgtatat tctttcgttg aaaaatatca aaaaatagtg ccatttgcgt 480
atgaatatga atcttttact tcttcttttt gatatctgcc aaatgggttt ctgcgattcc 540
gatcttttat caccacaact tgtatcgtta ggactaatat ttatatccgg agttagaaat 600
atttttcttt tgtcttttgc acccctttct atttgatttc tgattagggt tgttctatcg 660
ttttcctcct cccgctcttc aaaagaatcg atcaatttgt atgatcgtcg aggaatcttt 720
ttaccgattt cttctattcc aatagatttt ttttgaattg tttgattatt tggatcagtt 780
gtaattacat cgaatagaaa tttcaaacat tttgttttat tttctgaatc aaatcttctt 840
tgttcggata ttaaaacaag ctttttaaaa ttaagattaa aaccagttgt tgatttccta 900
gctaattcac tgatagaggt caagaaagga ccaatgtctg tcgataatga ttctccatta 960
aatatatcca ttttatgttc aagtttttgg taatcagtag gaagcatatc atgaatctta 1020
tttatccaaa ccattcctat agaatcttct gtccccattc cacctaaaat cctaggaatt 1080
cgttgatggt tggaatagat tcgtagaccg ggacgactga tacgctttaa aatatttcta 1140
tatgttcctt tcctattcct tctatgtcgc agggttgaaa ccaaaaaata tttgttgttt 1200
tccctatgtt tcctaacatt ttcaataaac ccttcttgta gaagtatttt aacaatgttt 1260
tcggcgatat tagtagatgc tattcgaacc gttccttttt tatccatgtt agcatttctt 1320
atagaagtta ttatatcggc aatagtgtcc ctacccatga cgaactaaaa ttatgggtgc 1380
cttccagttt tgatataatc aacatgttac tttttttttt tcatctacta gtatttataa 1440
gacttcagga gctaatgaga ctattttagt gaaattcaac tgtctcaatt cccgcgcgat 1500
cgctccaaaa actcgagttc cttttggatt tccttcttga tcaatgacaa ctgctgcatt 1560
gtcatcatat cgtattatca taccgttgtc gcgcttgagt tctttacatg tacgtacaat 1620
tacagctctg atcacttctg atctttcgag aggcatattg ggcactgctt ctttgattac 1680
agcaacaata acgtcaccaa tatgagcata tcgttgatta ctagctccta tgattcgaat 1740
acacatcaat tctcgagccc cgctgttgtc cgctacattc aaatgggtct gaggttgaat 1800
catatcattt tttttttaac aaagatcaaa gtgaaattca aaaaaaaaaa ctgggtgggc 1860
cattcagcgt atgttcgccc cctcgcccat attcatattg aaggttagtc agtcatttgg 1920
ataaacttta tgccccatat ctatgatatt tggattctca catgatgcat tctgagtcga 1980
aatgcgaaat aaaagagaag tctctacctt ccctaaagta aatataaata aagatagggt 2040
agacaaaatg actaattcta tgtggatcct gaatcctaaa aaacaggggg gttcttggta 2100
ttaattccat cgctggaatt aaagctcctg agactggggt gggacaaaat caaacaaaat 2160
agtaacaacg aattgatatg aacccatttt gctttgtact tatacaagac aggatagcat 2220
cccataagaa gatcctttta aattggcttt ggatatgatt catgatcccc agggcagaat 2280
taagacagat caaactaaag atattaagca taacaaacat tttgttcttg gagaaaattt 2340
cattattatt gggttattga tataagggga aaatgaagaa agaagggaaa gtaggccgca 2400
aaatctttct ttttctttga actccctcaa tcaaaaatcc ttcaattctc aaatgagaat 2460
agaaggaatc ataccttaca tacaatatct taattgaatt atatgtaatg atcaataaat 2520
tcattttgat tctacatcta catgtgtaga atcatagcaa caaccaattc aatagatatt 2580
ggggctggaa ttgacgaaca accaataccg atattagtgt ttatcagtgt cgaatagaaa 2640
taaaaatggg gcgtggccaa gtggtaaggc aacgggtttt ggtcccgtta ctcggaggt 2699
<210> 37
<211> 2698
<212> DNA
<213> Phoebe sheareri
<400> 37
gaagggattg atgaaacaaa tcaaaaaaaa attcacttta tttcgactat aaaaaagtct 60
ctttctaata ttagtaataa taaatcaaag atttctggtg acctatattc cttttcacaa 120
gcatctgtat tttacaaatt atcacaaatc caagctatta agaagtctca tttgagatct 180
ctacttcaat atcgcgaagc atatcttatt cttaaggaaa gaatcaggaa tttttttgga 240
acacgaagaa tatttgattc caaatcaagg cataaaaaac ttcagaattc tggaatgaat 300
gaatggaaaa actggttaag gggtcattat caatacaatt tatctcagga tttatcgata 360
atcttttttg ttgattcaag gaaaagttgt acattgactc tagaaatatt aacagtactt 420
agaaagatat gtatgtatat tctttcgttg aaaaatgtca aaaaatagtg ccatttgcgt 480
atgaatatga atctgttact tcttcttttt gatatctgcc aaatgggttt ctgcgattcc 540
gatcttttat caccacaact tgtatcgtta ggactaatat ttatatccgg agttagaaat 600
atttttcttt tgtcttttgc acccctttct atttgatttc tgattagggt tgttctatcg 660
ttttcctcct cccgctcttc aaaagaatcg atcaatttgt atgatcgtcg aggaatcttt 720
ttaccgattt cttctattcc aatagatttt ttttgaattg tttgattatt tggatcagtt 780
gtaattacat cgaatagaaa tttcaaacat tttgttttat tttctgaatc aaatcttctt 840
tgttcggata ttaaaacaag ctttttaaaa ttaagattaa aaccagttgt tgatttccta 900
gctaattcac tgatagaggt caagaaagga ccaatgtctg tcgataatga ttctccatta 960
aatatatcca ttttatgttc aagtttttgg taatcagtag gaagcatatc atgaatctta 1020
tttatccaaa ccattcctat agaatcttct gtccccattc cacctaaaat cctaggaatt 1080
cgttgatggt tggaatagat tcgtagaccg ggacgactga tacgctttaa aatatttcta 1140
tatgttcctt tcctattcct tctatgtcgc agggttgaaa ccaaaaaata tttgttgttt 1200
tccctatgtt tcctaacatt ttcaataaac ccttcttgta gaagtatttt aacaatgttt 1260
tcggcgatat tagtagatgc tattcgaacc gttccttttt tatccatgtt agcatttctt 1320
atagaagtta ttatatcggc aatagtgtcc ctacccatga cgaactaaaa ttatgggtgc 1380
cttccagttt tgatataatc aacatgttac tttttttttt tcatctacta gtatttataa 1440
gacttcagga gctaatgaga ctattttagt gaaattcaac tgtctcaatt cccgcgcgat 1500
cgctccaaaa actcgagttc cttttggatt tccttcttga tcaatgacaa ctgctgcatt 1560
gtcatcatat cgtattatca taccgttgtc gcgcttgagt tctttacatg tacgtacaat 1620
tacagctctg atcacttctg atctttcgag aggcatattg ggcactgctt ctttgattac 1680
agcaacaata acgtcaccaa tatgagcata tcgttgatta ctagctccta tgattcgaat 1740
acacatcaat tctcgagccc cgctgttgtc cgctacattc aaatgggtct gaggttgaat 1800
catatcattt tttttttaac aaagatcaaa gtgaaattca aaaaaaaaac tgggtgggcc 1860
attcagcgta tgttcgcccc ctcgcccata ttcatattga aggttagtta gtcatttgga 1920
taaactttat gccccatatc tatgatattt ggattctcac atgatgcatt ctgagtcgaa 1980
atgcgaaata aaagagaagt ctctaccttc cctaaagtaa atataaataa agatagggta 2040
gacaaaatga ctaattctat gtggatcctg aatcctaaaa aacagggggg ttcttggtat 2100
taattccatc gctggaatta aagctcctga gactggggtg ggacaaaatc aaacaaaata 2160
gtaacaacga attgatatga acccattttg ctttgtactt atacaagaca ggatagcatc 2220
ccataagaag atccttttaa attggctttg gatatgattc atgatcccca gggcagaatt 2280
aagacagatc aaactaaaga tattaagcat aacaaacatt ttgttcttgg agaaaatttc 2340
attattattg ggttattgat ataaggggaa aatgaagaaa gaagggaaag taggccgcaa 2400
aatctttctt tttctttgaa ctccctcaat caaaaatcct tcaattctca aatgagaata 2460
gaaggaatca taccttacat acaatatctt aattgaatta tatgtaatga tcaataaatt 2520
cattttgatt ctacatctac atgtgtagaa tcatagcaac aaccaattca atagatattg 2580
gggctggaat tgacgaacaa ccaataccga tattagtgtt tatcggtgtc gaatagaaat 2640
aaaaatgggg cgtggccaag tggtaaggca acgggttttg gtcccgttac tcggaggt 2698
<210> 38
<211> 2699
<212> DNA
<213> Phoebe omeiensis
<400> 38
gaagggattg atgaaacaaa tcaaaaaaaa attcacttta tttcgactat aaaaaagcct 60
ctttctaata ttagtaataa taaatcaaag atttctggtg acctatattc cttttcacaa 120
gcatctgtat tttacaaatt atcacaaatc caagctatta agaagtatca tttgagatct 180
ctacttcaat atcgcgaagc atatcttatt cttaaggaaa gaatcaggaa tttttttgga 240
acacgaagaa tatttgattc caaatcaagg cataaaaaac ttcagaattc tggaatgaat 300
gaatggaaaa actggttaag gggtcattat caatacaatt tatctcagga tttatcgata 360
atcttttttg ttgattcaag gaaaagttgt acattgactc tagaaatatt aacagtactt 420
agaaagatat gtatgtatat tctttcgttg aaaaatgtca aaaaatagtg ccatttgcgt 480
atgaatatga atctgttact tcttcttttt gatatctgcc aaatgggttt ctgcgattcc 540
gatcttttat caccacaact tgtatcgtta ggactaatat ttatatccgg agttagaaat 600
atttttcttt tgtcttttgc acccctttct atttgatttc tgattagggt tgttctatcg 660
ttttcctcct cccgctcttc aaaagaatcg atcaatttgt atgatcgtcg aggaatcttt 720
ttaccgattt cttctattcc aatagatttt ttttgaattg tttgattatt tggatcagtt 780
gtaattacat cgaatagaaa tttcaaacat tttgttttat tttctgaatc aaatcttctt 840
tgttcggata ttaaaacaag cttttttaaa ttaagattaa aaccagttgt tgatttccta 900
gctaattcac tgatagaggt caagaaagga ccaatgtctg tcgataatga ttctccatta 960
aatatatcca ttttatgttc aagtttttgg taatcagtag gaagcatatc atgaatctta 1020
tttatccaaa ccattcctat agaatcttct gtccccatcc cacctaaaat cctaggaatt 1080
cgttgatggt tggaatagat tcgtagaccg ggacgactga tacgctttaa aatatttcta 1140
tatgttcctt tcctattcct tctatgtcgc agggttgaaa ccaaaaaata tttgttgttt 1200
tctctatgtt tcctaacatt ttcaataaac ccttcttgta gaagtatttt aacaatgttt 1260
tcggcgatat tagtagatgc tattcgaacc gttccttttt tatccatgtt agcatttctt 1320
atagaagtta ttatatcggc aatagtgtcc ctacccatga cgaactaaaa ttatgggtgc 1380
cttccagttt tgatataatc aacatgttcc tttttttttt tcatctacta gtatttataa 1440
gacttcagga gctaatgaga ctattttagt gaaattcaac tgtctcaatt cccgcgcgat 1500
cgctccaaaa actcgagttc cttttggatt tccttcttga tcaatgacaa ctgctgcatt 1560
gtcatcatat cgtattatca taccgttgtc gcgtttgagt tctttacatg tacgtacaat 1620
tacagctctg atcacttctg atctttcgag aggcatattg ggcactgctt ctttgattac 1680
agcaacaata acgtcaccaa tatgagcata tcgttgatta ctagctccta tgattcgaat 1740
acacatcaat tctcgagccc cgctgttgtc cgctacattc aaatgggtct gaggttgaat 1800
catatcattt tttttttaac aaagatcaaa gtgaaattca aaaaaaaaaa ctgggtgggc 1860
cattcagcgt atgttcgccc cctcgcccat attcatattg aaggttagtt agtcatttgg 1920
ataaacttta tgccccatat ctatgatatt tggattctca catgatgcat tctgagtcga 1980
aacgcgaaat aaaagagaag tctctacctt ccctaaagta aatataaata aggatagggt 2040
agacaaaatg actaattcta tgtggatcct gaatcctaaa aaacgggggg gttcttggta 2100
ttaattccat cgctggaatt aaagctcctg agactggggt gggacaaaat caaacaaaat 2160
agtaacaacg aattgatatg aacccatttt gctttgtact tatacaagac aggatagcat 2220
cccataagaa gatcctttta aattggcttt ggatatgatt catgatcccc agggcagaat 2280
taagacagat caaactaaag atattaagca taacaaacat tttgttcttg gagaaaattt 2340
cattattatt gggttattga tataagggga aaatgaagaa agaagggaaa gtaggccgca 2400
aaatctttct ttttctttga actccctcaa tcaaaaatcc ttcaattctc aaatgagaat 2460
agaaggaatc ataccttaca tacaatatct taattgaatt atatgtaatg atcaataaat 2520
tcattttgat tctacatcta catgtgtaga atcatagcaa caaccaattc aatagatatt 2580
ggggctggaa ttgacgaaca accaataccg atattagtgt ttatcggtgt cgaatagaaa 2640
taaaaatggg gcgtggccaa gtggtaaggc aacgggtttt ggtcccgtta ctcggaggt 2699
<210> 38
<211> 2700
<212> DNA
<213> Phoebe neurantha
<400> 38
gaagggattg atgaaacaaa tcaaaaaaaa attaacttta tttcgactat aaaaaagtct 60
ctttctaata ttagtaataa taaatcaaag atttctggtg acctatattc cttttcacaa 120
gcatctgtat tttacaaatt atcacaaatc caagctatta agaagtatca tttgagatct 180
ctacttcaat atcgcgaagc atatcttatt cttaaggaaa gaatcaggaa tttttttgga 240
acacgaagaa tatttgattc caaatcaagg cataaaaaac ttccgaattc tggaatgaat 300
gaatggaaaa actggttaag gggtcattat caatacaatt tatctcagga tttatcgata 360
atcttttttg ttgattcaag gaaaagttgt acattgactc tagaaatatt aacagtacat 420
agaaagatat gtatgtatat tctttcgttg aaaaatgtca aaaaatagtg ccatttgcgt 480
atgaatatga atctgttact tcttcttttt gatatctgcc aaataggttt ctgcgattcc 540
gatcttttat caccacaact tgtatcgtta ggactaatat ttatatccgg agttagaaat 600
atttttcttt tgtcttttgc acccctttct atttgatttc tgattagggt tgttctatcg 660
ttttcctcct cccgctcttc aaaagaatcg atcaatttgt atgatcgtcg aggaatcttt 720
ttaccgattt cttctattcc aatagatttt ttttgaattg tttgattatt tggatcagtt 780
gtaattacat cgaatagaaa tttcaaacat tttttttgat tttctgaatc aaatcttctt 840
tgttcggata ttaaaacaag ctttttaaaa ttaagattaa aaccagttgt tgatttccta 900
gctaattcac tgatagaggt caagaaagga ccaatgtctg tcgataatga ttctccatta 960
aatatatcca ttttatgttc aagtttttgg taatcagtag gaagcatatc atgaatctta 1020
tttatccaaa ccattcctat agaatcttct gtccccatcc cacctaaaat cctaggaatt 1080
cgttgatggt tggaatagat tcgtagaccg ggacgactga tacgctttaa aatatttcta 1140
tatgttcctt tcctattcct tctatgtcgc agggttgaaa ccaaaaaata tttgttgttt 1200
tctctatgtt tcctaacatt ttcaataaac ccttcttgta gaagtatttt aacaatgttt 1260
tcggcgatat tagtagatgc tattcgaacc gttccttttt tatccatgtt agcatttctt 1320
atagaagtta ttatatcggc aatagtgtcc ctacccatga cgaactaaaa ttatgggtgc 1380
cttccagttt tgatataatc aacatgttcc tttttttttt tcatctacta gtatttataa 1440
gacttcagga gctaatgaga ctattttagt gaaattcaac tgtctcaatt cccgcgcgat 1500
cgctccaaaa actcgagttc cttttggatt tccttcttga tcaatgacaa ctgctgcatt 1560
gtcatcatat cgtattatca taccgttgtc gcgtttgagt tctttacatg tacgtacaat 1620
tacagctctg atcacttctg atctttcgag aggcatattg ggcactgctt ctttgattac 1680
agcaacaata acgtcaccaa tatgagcata tcgttgatta ctagctccta tgattcgaat 1740
acacatcaat tctcgagccc cgctgttgtc cgctacattc aaatgggtct gaggttgaat 1800
catatcattt tttttttaac aaagatcaaa gtgaaattca aaaaaaaaaa actgggtggg 1860
ccattcagcg tatgttcgcc ccctcgccca tattcatatt gaaggttagt tagtcatttg 1920
gataaacttt atgccccata tctatgatat ttggattctc acatgatgca ttctgagtcg 1980
aaacgcgaaa taaaagagaa gtctctacct tccctaaagt aaatataaat aaagataggg 2040
tagacaaaat gactaattct atgtggatcc tgaatcctaa aaaacggggg ggttcttggt 2100
attaattcca tcgctggaat taaagctcct gagactgggg tgggacaaaa tcaaacaaaa 2160
tagtaacaac gaattgatat gaacccattt tgctttgtac ttatacaaga caggatagca 2220
tcccataaga agatcctttt aaattggctt tggatatgat tcatgatccc cagggcagaa 2280
ttaagacaga tcaaactaaa gatattaagc ataacaaaca ttttgttctt ggagaaaatt 2340
tcattattat tgggttattg atataagggg aaaatgaaga aagaagggaa agtaggccgc 2400
aaaatctttc tttttctttg aactccctca atcaaaaatc cttcaattct caaatgagaa 2460
tagaaggaat cataccttac atacaatatc ttaattgaat tatatgtaat gatcaataaa 2520
ttcattttga ttctacatct acatgtgtag aatcatagca acaaccaatt caatagatat 2580
tggggctgga attgacgaac aaccaatacc gatattagtg tttatcggtg tcgaatagaa 2640
ataaaaatgg ggcgtggcca agtggtaagg caacgggttt tggtcccgtt actcggaggt 2700
<210> 39
<211> 2700
<212> DNA
<213> Phoebe neurantha
<400> 39
gaagggattg atgaaacaaa tcaaaaaaaa attaacttta tttcgactat aaaaaagtct 60
ctttctaata ttagtaataa taaatcaaag atttctggtg acctatattc cttttcacaa 120
gcatctgtat tttacaaatt atcacaaatc caagctatta agaagtatca tttgagatct 180
ctacttcaat atcgcgaagc atatcttatt cttaaggaaa gaatcaggaa tttttttgga 240
acacgaagaa tatttgattc caaatcaagg cataaaaaac ttccgaattc tggaatgaat 300
gaatggaaaa actggttaag gggtcattat caatacaatt tatctcagga tttatcgata 360
atcttttttg ttgattcaag gaaaagttgt acattgactc tagaaatatt aacagtacat 420
agaaagatat gtatgtatat tctttcgttg aaaaatgtca aaaaatagtg ccatttgcgt 480
atgaatatga atctgttact tcttcttttt gatatctgcc aaataggttt ctgcgattcc 540
gatcttttat caccacaact tgtatcgtta ggactaatat ttatatccgg agttagaaat 600
atttttcttt tgtcttttgc acccctttct atttgatttc tgattagggt tgttctatcg 660
ttttcctcct cccgctcttc aaaagaatcg atcaatttgt atgatcgtcg aggaatcttt 720
ttaccgattt cttctattcc aatagatttt ttttgaattg tttgattatt tggatcagtt 780
gtaattacat cgaatagaaa tttcaaacat tttttttgat tttctgaatc aaatcttctt 840
tgttcggata ttaaaacaag ctttttaaaa ttaagattaa aaccagttgt tgatttccta 900
gctaattcac tgatagaggt caagaaagga ccaatgtctg tcgataatga ttctccatta 960
aatatatcca ttttatgttc aagtttttgg taatcagtag gaagcatatc atgaatctta 1020
tttatccaaa ccattcctat agaatcttct gtccccattc cacctaaaat cctaggaatt 1080
cgttgatggt tggaatagat tcgtagaccg ggacgactga tacgctttaa aatatttcta 1140
tatgttcctt tcctattcct tctatgtcgc agggttgaaa ccaaaaaata tttgttgttt 1200
tctctatgtt tcctaacatt ttcaataaac ccttcttgta gaagtatttt aacaatgttt 1260
tcggcgatat tagtagatgc tattcgaacc gttccttttt tatccatgtt agcatttctt 1320
atagaagtta ttatatcggc aatagtgtcc ctacccatga cgaactaaaa ttatgggtgc 1380
cttccagttt tgatataatc aacatgttcc tttttttttt tcatctacta gtatttataa 1440
gacttcagga gctaatgaga ctattttagt gaaattcaac tgtctcaatt cccgcgcgat 1500
cgctccaaaa actcgagttc cttttggatt tccttcttga tcaatgacaa ctgctgcatt 1560
gtcatcatat cgtattatca taccgttgtc gcgtttgagt tctttacatg tacgtacaat 1620
tacagctctg atcacttctg atctttcgag aggcatattg ggcactgctt ctttgattac 1680
agcaacaata acgtcaccaa tatgagcata tcgttgatta ctagctccta tgattcgaat 1740
acacatcaat tctcgagccc cgctgttgtc cgctacattc aaatgggtct gaggttgaat 1800
catatcattt tttttttaac aaagatcaaa gtgaaattca aaaaaaaaaa actgggtggg 1860
ccattcagcg tatgttcgcc ccctcgccca tattcatatt gaaggttagt tagtcatttg 1920
gataaacttt atgccccata tctatgatat ttggattctc acatgatgca ttctgagtcg 1980
aaacgcgaaa taaaagagaa gtctctacct tccctaaagt aaatataaat aaagataggg 2040
tagacaaaat gactaattct atgtggatcc tgaatcctaa aaaacggggg ggttcttggt 2100
attaattcca tcgctggaat taaagctcct gagactgggg tgggacaaaa tcaaacaaaa 2160
tagtaacaac gaattgatat gaacccattt tgctttgtac ttatacaaga caggatagca 2220
tcccataaga agatcctttt aaattggctt tggatatgat tcatgatccc cagggcagaa 2280
ttaagacaga tcaaactaaa gatattaagc ataacaaaca ttttgttctt ggagaaaatt 2340
tcattattat tgggttattg atataagggg aaaatgaaga aagaagggaa agtaggccgc 2400
aaaatctttc tttttctttg aactccctca atcaaaaatc cttcaattct caaatgagaa 2460
tagaaggaat cataccttac atacaatatc ttaattgaat tatatgtaat gatcaataaa 2520
ttcattttga ttctacatct acatgtgtag aatcatagca acaaccaatt caatagatat 2580
tggggctgga attgacgaac aaccaatacc gatattagtg tttatcggtg tcgaatagaa 2640
ataaaaatgg ggcgtggcca agtggtaagg caacgggttt tggtcccgtt actcggaggt 2700
<210> 40
<211> 2700
<212> DNA
<213> Phoebe neurantha
<400> 40
gaagggattg atgaaacaaa tcaaaaaaaa attaacttta tttcgactat aaaaaagtct 60
ctttctaata ttagtaataa taaatcaaag atttctggtg acctatattc cttttcacaa 120
gcatctgtat tttacaaatt atcacaaatc caagctatta agaagtatca tttgagatct 180
ctacttcaat atcgcgaagc atatcttatt cttaaggaaa gaatcaggaa tttttttgga 240
acacgaagaa tatttgattc caaatcaagg cataaaaaac ttccgaattc tggaatgaat 300
gaatggaaaa actggttaag gggtcattat caatacaatt tatctcagga tttatcgata 360
atcttttttg ttgattcaag gaaaagttgt acattgactc tagaaatatt aacagtacat 420
agaaagatat gtatgtatat tctttcgttg aaaaatgtca aaaaatagtg ccatttgcgt 480
atgaatatga atctgttact tcttcttttt gatatctgcc aaataggttt ctgcgattcc 540
gatcttttat caccacaact tgtatcgtta ggactaatat ttatatccgg agttagaaat 600
atttttcttt tgtcttttgc acccctttct atttgatttc tgattagggt tgttctatcg 660
ttttcctcct cccgctcttc aaaagaatcg atcaatttgt atgatcgtcg aggaatcttt 720
ttaccgattt cttctattcc aatagatttt ttttgaattg tttgattatt tggatcagtt 780
gtaattacat cgaatagaaa tttcaaacat tttttttgat tttctgaatc aaatcttctt 840
tgttcggata ttaaaacaag ctttttaaaa ttaagattaa aaccagttgt tgatttccta 900
gctaattcac tgatagaggt caagaaagga ccaatgtctg tcgataatga ttctccatta 960
aatatatcca ttttatgttc aagtttttgg taatcagtag gaagcatatc atgaatctta 1020
tttatccaaa ccattcctat agaatcttct gtccccatcc cacctaaaat cctaggaatt 1080
cgttgatggt tggaatagat tcgtagaccg ggacgactga tacgctttaa aatatttcta 1140
tatgttcctt tcctattcct tctatgtcgc agggttgaaa ccaaaaaata tttgttgttt 1200
tctctatgtt tcctaacatt ttcaataaac ccttcttgta gaagtatttt aacaatgttt 1260
tcggcgatat tagtagatgc tattcgaacc gttccttttt tatccatgtt agcatttctt 1320
atagaagtta ttatatcggc aatagtgtcc ctacccatga cgaactaaaa ttatgggtgc 1380
cttccagttt tgatataatc aacatgttcc tttttttttt tcatctacta gtatttataa 1440
gacttcagga gctaatgaga ctattttagt gaaattcaac tgtctcaatt cccgcgcgat 1500
cgctccaaaa actcgagttc cttttggatt tccttcttga tcaatgacaa ctgctgcatt 1560
gtcatcatat cgtattatca taccgttgtc gcgtttgagt tctttacatg tacgtacaat 1620
tacagctctg atcacttctg atctttcgag aggcatattg ggcactgctt ctttgattac 1680
agcaacaata acgtcaccaa tatgagcata tcgttgatta ctagctccta tgattcgaat 1740
acacatcaat tctcgagccc cgctgttgtc cgctacattc aaatgggtct gaggttgaat 1800
catatcattt tttttttaac aaagatcaaa gtgaaattca aaaaaaaaaa actgggtggg 1860
ccattcagcg tatgttcgcc ccctcgccca tattcatatt gaaggttagt tagtcatttg 1920
gataaacttt atgccccata tctatgatat ttggattctc acatgatgca ttctgagtcg 1980
aaacgcgaaa taaaagagaa gtctctacct tccctaaagt aaatataaat aaagataggg 2040
tagacaaaat gactaattct atgtggatcc tgaatcctaa aaaacggggg ggttcttggt 2100
attaattcca tcgctggaat taaagctcct gagactgggg tgggacaaaa tcaaacaaaa 2160
tagtaacaac gaattgatat gaacccattt tgctttgtac ttatacaaga caggatagca 2220
tcccataaga agatcctttt aaattggctt tggatatgat tcatgatccc cagggcagaa 2280
ttaagacaga tcaaactaaa gatattaagc ataacaaaca ttttgttctt ggagaaaatt 2340
tcattattat tgggttattg atataagggg aaaatgaaga aagaagggaa agtaggccgc 2400
aaaatctttc tttttctttg aactccctca atcaaaaatc cttcaattct caaatgagaa 2460
tagaaggaat cataccttac atacaatatc ttaattgaat tatatgtaat gatcaataaa 2520
ttcattttga ttctacatct acatgtgtag aatcatagca acaaccaatt caatagatat 2580
tggggctgga attgacgaac aaccaatacc gatattagtg tttatcggtg tcgaatagaa 2640
ataaaaatgg ggcgtggcca agtggtaagg caacgggttt tggtcccgtt actcggaggt 2700
<210> 41
<211> 2700
<212> DNA
<213> Phoebe neurantha
<400> 41
gaagggattg atgaaacaaa tcaaaaaaaa attaacttta tttcgactat aaaaaagtct 60
ctttctaata ttagtaataa taaatcaaag atttctggtg acctatattc cttttcacaa 120
gcatctgtat tttacaaatt atcacaaatc caagctatta agaagtatca tttgagatct 180
ctacttcaat atcgcgaagc atatcttatt cttaaggaaa gaatcaggaa tttttttgga 240
acacgaagaa tatttgattc caaatcaagg cataaaaaac ttccgaattc tggaatgaat 300
gaatggaaaa actggttaag gggtcattat caatacaatt tatctcagga tttatcgata 360
atcttttttg ttgattcaag gaaaagttgt acattgactc tagaaatatt aacagtacat 420
agaaagatat gtatgtatat tctttcgttg aaaaatgtca aaaaatagtg ccatttgcgt 480
atgaatatga atctgttact tcttcttttt gatatctgcc aaataggttt ctgcgattcc 540
gatcttttat caccacaact tgtatcgtta ggactaatat ttatatccgg agttagaaat 600
atttttcttt tgtcttttgc acccctttct atttgatttc tgattagggt tgttctatcg 660
ttttcctcct cccgctcttc aaaagaatcg atcaatttgt atgatcgtcg aggaatcttt 720
ttaccgattt cttctattcc aatagatttt ttttgaattg tttgattatt tggatcagtt 780
gtaattacat cgaatagaaa tttcaaacat tttttttgat tttctgaatc aaatcttctt 840
tgttcggata ttaaaacaag ctttttaaaa ttaagattaa aaccagttgt tgatttccta 900
gctaattcac tgatagaggt caagaaagga ccaatgtctg tcgataatga ttctccatta 960
aatatatcca ttttatgttc aagtttttgg taatcagtag gaagcatatc atgaatctta 1020
tttatccaaa ccattcctat agaatcttct gtccccatcc cacctaaaat cctaggaatt 1080
cgttgatggt tggaatagat tcgtagaccg ggacgactga tacgctttaa aatatttcta 1140
tatgttcctt tcctattcct tctatgtcgc agggttgaaa ccaaaaaata tttgttgttt 1200
tctctatgtt tcctaacatt ttcaataaac ccttcttgta gaagtatttt aacaatgttt 1260
tcggcgatat tagtagatgc tattcgaacc gttccttttt tatccatgtt agcatttctt 1320
atagaagtta ttatatcggc aatagtgtcc ctacccatga cgaactaaaa ttatgggtgc 1380
cttccagttt tgatataatc aacatgttcc tttttttttt tcatctacta gtatttataa 1440
gacttcagga gctaatgaga ctattttagt gaaattcaac tgtctcaatt cccgcgcgat 1500
cgctccaaaa actcgagttc cttttggatt tccttcttga tcaatgacaa ctgctgcatt 1560
gtcatcatat cgtattatca taccgttgtc gcgtttgagt tctttacatg tacgtacaat 1620
tacagctctg atcacttctg atctttcgag aggcatattg ggcactgctt ctttgattac 1680
agcaacaata acgtcaccaa tatgagcata tcgttgatta ctagctccta tgattcgaat 1740
acacatcaat tctcgagccc cgctgttgtc cgctacattc aaatgggtct gaggttgaat 1800
catatcattt tttttttaac aaagatcaaa gtgaaattca aaaaaaaaaa actgggtggg 1860
ccattcagcg tatgttcgcc ccctcgccca tattcatatt gaaggttagt tagtcatttg 1920
gataaacttt atgccccata tctatgatat ttggattctc acatgatgca ttctgagtcg 1980
aaacgcgaaa taaaagagaa gtctctacct tccctaaagt aaatataaat aaagataggg 2040
tagacaaaat gactaattct atgtggatcc tgaatcctaa aaaacggggg ggttcttggt 2100
attaattcca tcgctggaat taaagctcct gagactgggg tgggacaaaa tcaaacaaaa 2160
tagtaacaac gaattgatat gaacccattt tgctttgtac ttatacaaga caggatagca 2220
tcccataaga agatcctttt aaattggctt tggatatgat tcatgatccc cagggcagaa 2280
ttaagacaga tcaaactaaa gatattaagc ataacaaaca ttttgttctt ggagaaaatt 2340
tcattattat tgggttattg atataagggg aaaatgaaga aagaagggaa agtaggccgc 2400
aaaatctttc tttttctttg aactccctca atcaaaaatc cttcaattct caaatgagaa 2460
tagaaggaat cataccttac atacaatatc ttaattgaat tatatgtaat gatcaataaa 2520
ttcattttga ttctacatct acatgtgtag aatcatagca acaaccaatt caatagatat 2580
tggggctgga attgacgaac aaccaatacc gatattagtg tttatcggtg tcgaatagaa 2640
ataaaaatgg ggcgtggcca agtggtaagg caacgggttt tggtcccgtt actcggaggt 2700
<210> 42
<211> 2712
<212> DNA
<213> Machilus balansae
<400> 42
gaagggattg atgaaacaaa taaaaaaaaa attcacttta tttcgactat aaaaaagtct 60
ctttctaata ttagtaataa taaatcaaag atttctggtg acccatattc cttttcacaa 120
gcatctgtat tttacaaatt atcacaaatc caagctatca agaagtatca tttgagatct 180
ctacttcaat atcgcgaagc atatcttatt cttaaggata gaatcaggaa tttttttgga 240
acacgaagaa tatttgattc caaatcaagg cataaaaaac ttccgaattc tggaatgaat 300
gaatggaaaa actggttaag gggtcattat caatacaatt tatctcagga tttatcgata 360
atcttttttg ttgattcaag gaaaagttgt acattgactc tagaaatatt aaccgtacat 420
agaaagatat gtatgtatat tctttcgttg aaaaatgtca aaaaatagtg ccatttgcgt 480
atgaatatga atctgttact tcttcttttt gatatctgcc aaataggttt ctgcgattcc 540
gatcttttat caccacaact tgtatcgtta ggactcatat ttatatccgg agttagaaat 600
atttttcttt tgtcttttgc acccctttct atttgatttc tgattagggt tgttctatcg 660
ttttcctcct cccgctcttc aaaagaatcg atcaatttgt atgatcgtcg aggaatcttt 720
ttaccgattt cttctattcc aatagatttt ttttgaattg tttgattatt tggatcagtt 780
gtaattacat cgaatagaaa tttcaaacat tttgttttat tttctgaatc aaatcttctt 840
tgttcggata ttaaaacaag ctttttcaaa ttaagattaa aaccagttgt tgatttccta 900
gctaattcac tgatagaggt caagaaagga ccaatgtctg tcgataatga ttctccatta 960
aatatatcca ttttatgttc aagtttttgg taatcagtag gaagcatatc atgaatctta 1020
tttatccaaa ccattcctat ggaatcttct gtccccattc cacctaaaat cctaggaatt 1080
cgttgatggt tggaatagat tcgtagaccg ggacgactga tacgctttaa aatagttcta 1140
tatgttcctt tcctattcct tctatgtcgc agggttgaaa ccaaaaaata tttgttgttt 1200
tccctatgtt tcctaacatt ttcaataaac ccttcttgta gaagtatttt aacaatgttt 1260
tcggcgatat tagtagatgc tattcgaacc gttccttttt tatccatgtt agcatttctt 1320
atagaagtta ttatatcggc aatagtgtcc ctacccatga cgaactaaaa ttatgggtgc 1380
cttccagttt tgatataatc aacatgttcc tttttttttt tcatctacta gtatttataa 1440
gacttcagga gctaatgaga ctattttagt gaaattcaac tgtctcaatt cccgcgcgat 1500
cgctccaaaa actcgagttc cttttggatt tccttcttga tcaatgacaa ctgctgcatt 1560
gtcatcatat cgtattatca taccgttgtc gcgtttgagt tctttacatg tacgtacaat 1620
tacagctctg atcacttctg atctttcgag aggcatattg ggcactgctt ctttgattac 1680
agcaacaata acgtcaccaa tatgagcata tcgttgatta ctagctccta tgatttgaat 1740
acacatcaat tctcgagccc cgctgttgtc cgctacattc aaaagggttt gagggtgaat 1800
catatcattt tttttttaac aaagatcaaa gtgaaattca aaaaaaaaaa aaaaaaactg 1860
ggtgggccat tcagcgtatg ttcgccccct cgcccatatt catattgaag gttagttagt 1920
catttggata aactttatgt cccatatcta tgatatttgg attctcacat gatgcattct 1980
gagtcgaaat gcgaaataaa agagaagtct cgaccttccc taaagtaaat ataaataaag 2040
atagggtaga caaaatgact aattctatgt ggatcctgaa tcctaaaaaa cgggggggtt 2100
cttggtatta attccagcga tggaattaaa gctcctgaga ctggggtggg acaaaatcaa 2160
acaaaatagt aacaacgaat tgatatgaac ccattttgct ttgtacttat acaagtacaa 2220
gacaggatag catcccataa gaagatcctt ttaaattggc tttggatatg attcatgatc 2280
cccagggcag aattaagaca gatcaaacta aagatattaa gcataacaaa cattttgttc 2340
ttggagaaaa tttcattatt attgggttat tgatataagg ggaaaatgaa gaaagaaggg 2400
aaagtaggcc gcaaaatatt tctttttctt tgaactccct caatcaaaaa tccttccatt 2460
ctcaaatgag aatagaagga atcatacctt acatacaata tcttaattga attatatgta 2520
atgatcaata aattcatttt gattctacat ctacatgtgt aaaatcatag caacaaccaa 2580
ttcaatagat attggggctg gaattgacga acaaccaata ccgatattag tgtttatcgg 2640
tgtcgaatag aaataaaaat ggggcgtggc caagtggtaa ggcaacgggt tttggtcccg 2700
ttactcggag gt 2712
<210> 43
<211> 2697
<212> DNA
<213> Machilus thunbergii
<400> 43
gaagggattg atgaaacaaa tcaaaaaaaa attcaattta tttcgactat aaaaaagtct 60
ctttctaata ttagtaataa taaatcaaaa atttctggtg acccatattc cttttcacaa 120
gcatctgtat tttacaaatt atcacaaatc gaagctatca agaagtatca tttgagatct 180
ctacttcaat atcgcgaagc atatcttatt cttaaggata gaatcaggaa tttttttgga 240
acacgaagaa tatttgattc caaatcaagg cataaaaaac ttccgaattc tggaatgaat 300
gaatggaaaa actggttaag gggtcattat caatacaatt tatctcagga tttatcgata 360
atcttttttg ttgattcaag gaaaagttgt acattgactc tagaaatatt aaccgtacat 420
agaaagatat gtatgtatat tctttcgttg aaaaatgtca aaaaatagtg ccatttgcgt 480
atgaatatga atctgttact tcttcttttt gatatctgcc aaataggttt ctgcgattcc 540
gatcttttat caccacaact tgtatcgtta ggactaatat ttatatccgg agttagaaat 600
atttttcttt tgtcttttgc acccctttct atttgatttc tgattagggt tgttctatcg 660
ttttcctcct cccgctcttc aaaagaatcg atcaatttgt atgatcgtcg aggaatcttt 720
ttaccgattt cttctattcc aatagatttt ttttgaattg tttgattatt tggatcagtt 780
gtaattacat cgaatagaaa tttcaaacat ttttttttat tttctgaatc aaatcttctt 840
tgttcggata ttaaaacaag ctttttcaaa ttaagattaa aaccagttgt tgatttccta 900
gctaattcac tgatagaggt caagaaagga ccaatgtctg tcgataatga ttctccatta 960
aatatatcca ttttatgttc aagtttttgg taatcagtag gaagcatatc atgaatctta 1020
tttatccaaa ccattcctat ggaatcttct gtccccattc cacctaaaat cctaggaatt 1080
cgttgatggt tggaatagat tcgtagaccg ggacgactga tacgctttaa aatagttcta 1140
tatgttcctt tcctattcct tctatgtcgc agggttgaaa ccaaaaaata tttgttgttt 1200
tccctatgtt tcctaacatt ttcaataaac ccttcttgta gaagtatttt aacaatgttt 1260
tcggcgatat tagtagatgc tattcgaacc gttccttttt tatccatgtt agcatttctt 1320
atagaagtta ttatatcggc aatagtgtcc ctacccatga cgaactaaaa ttatgggtgc 1380
cttccagttt tgatacaatc aacatgttcc tttttttttt tcatctacta gtatttataa 1440
gacttcagga gctaatgaga ctattttagt gaaattcaac tgtctcaatt cccgcgcgat 1500
cgctccaaaa acgcgagttc cttttggatt tccttcttga tcaatgacaa ctgctgcatt 1560
gtcatcatat cgtattatca taccgttgtc gcgtttgagt tctttacatg tacgtacaat 1620
tacagctctg atcacttctg atctttcgag aggcatattg ggcactgctt ctttgattac 1680
agcaacaata acgtcaccaa tatgagcata tcgttgatta ctagctccta tgattcgaat 1740
acacatcaat tctcgagccc cgctgttgtc cgctacattc aaatgggtct gaggttgaat 1800
catatcattt tttttttaac aaagatcaaa gtgaaattca aaaaaaaact gggtgggcca 1860
ttcagcgtat gttcgccccc tcgcccatat tcatattgaa ggttagttag tcatttggat 1920
aaactttatg tcccatatct atgatatttg gattctcaca tgatgcattc tgagtcgaaa 1980
tgcgaaataa aagagaagtc tcgaccttcc ctaaagtaaa tataaataaa gatagggtag 2040
acaaaatgac taattctatg tggatcctga atcctaaaaa acgggggggt tcttggtatt 2100
aattccagcg atggaattaa agctcctgag actggggtgg gacaaaatca aacaaaatag 2160
taacaacgaa ttgatatgaa cccattttgc tttgtactta tacaagacag gatagcatcc 2220
cataagaaga tccttttaaa ttggctttgg atatgattca tgatccccag ggcagaatta 2280
agacagatca aactaaagat attaagcata acaaacattt tgttcttgga gaaaatttca 2340
ttattattgg gttattgata taaggggaaa atgaagaaag aagggaaagt aggccgcaaa 2400
atatttcttt ttctttgaac tccctcaatc aaaaatcctt caattctcaa atgagaatag 2460
aaggaatcat accttacata caatatctta attgaattat atgtaatgat caataaattc 2520
attttgattc tacatctaca tgtgtaaaat catagcaaca accaattcaa tagatattgg 2580
ggctggaatt gacgaacaac caataccgat attagtgttt atcggtgtcg aatagaaata 2640
aaaatggggc gtggccaagt ggtaaggcaa cgggttttgg tcccgttact cggaggt 2697
<210> 44
<211> 2697
<212> DNA
<213> Machilus pauhoi
<400> 44
gaagggattg atgaaacaaa tcaaaaaaaa attcaattta tttcgactat aaaaaagtct 60
ctttctaata ttagtaataa taaatcaaaa atttctggtg acccatattc cttttcacaa 120
gcatctgtat tttacaaatt atcacaaatc gaagctatca agaagtatca tttgagatct 180
ctacttcaat atcgcgaagc atatcttatt cttaaggata gaatcaggaa tttttttgga 240
acacgaagaa tatttgattc caaatcaagg cataaaaaac ttccgaattc tggaatgaat 300
gaatggaaaa actggttaag gggtcattat caatacaatt tatctcagga tttatcgata 360
atcttttttg ttgattcaag gaaaagttgt acattgactc tagaaatatt aaccgtacat 420
agaaagatat gtatgtatat tctttcgttg aaaaatgtca aaaaatagtg ccatttgcgt 480
atgaatatga atctgttact tcttcttttt gatatctgcc aaataggttt ctgcgattcc 540
gatcttttat caccacaact tgtatcgtta ggactaatat ttatatccgg agttagaaat 600
atttttcttt tgtcttttgc acccctttct atttgatttc tgattagggt tgttctatcg 660
ttttcctcct cccgctcttc aaaagaatcg atcaatttgt atgatcgtcg aggaatcttt 720
ttaccgattt cttctattcc aatagatttt ttttgaattg tttgattatt tggatcagtt 780
gtaattacat cgaatagaaa tttcaaacat tttgttttat tttctgaatc aaatcttctt 840
tgttcggata ttaaaacaag ctttttcaaa ttaagattaa aaccagttgt tgatttccta 900
gctaattcac tgatagaggt caagaaagga ccaatgtctg tcgataatga ttctccatta 960
aatatatcca ttttatgttc aagtttttgg taatcagtag gaagcatatc atgaatctta 1020
tttatccaaa ccattcctat ggaatcttct gtccccattc cacctaaaat cctaggaatt 1080
cgttgatggt tggaatagat tcgtagaccg ggacgactga tacgctttaa aatagttcta 1140
tatgttcctt tcctattcct tctatgtcgc agggttgaaa ccaaaaaata tttgttgttt 1200
tccctatgtt tcctaacatt ttcaataaac ccttcttgta gaagtatttt aacaatgttt 1260
tcggcgatat tagtagatgc tattcgaacc gttccttttt tatccatgtt agcatttctt 1320
atagaagtta ttatatcggc aatagtgtcc ctacccatga cgaactaaaa ttatgggtgc 1380
cttccagttt tgatacaatc aacatgttcc tttttttttt tcatctacta gtatttataa 1440
gacttcagga gctaatgaga ctattttagt gaaattcaac tgtctcaatt cccgcgcgat 1500
cgctccaaaa acgcgagttc cttttggatt tccttcttga tcaatgacaa ctgctgcatt 1560
gtcatcatat cgtattatca taccgttgtc gcgtttgagt tctttacatg tacgtacaat 1620
tacagctctg atcacttctg atctttcgag aggcatattg ggcactgctt ctttgattac 1680
agcaacaata acgtcaccaa tatgagcata tcgttgatta ctagctccta tgattcgaat 1740
acacatcaat tctcgagccc cgctgttgtc cgctacattc aaatgggtct gaggttgaat 1800
catatcattt tttttttaac aaagatcaaa gtgaaattca aaaaaaaact gggtgggcca 1860
ttcagcgtat gttcgccccc tcgcccatat tcatattgaa ggttagttag tcatttggat 1920
aaactttatg tcccatatct atgatatttg gattctcaca tgatgcattc tgagtcgaaa 1980
tgcgaaataa aagagaagtc tcgaccttcc ctaaagtaaa tataaataaa gatagggtag 2040
acaaaatgac taattctatg tggatcctga atcctaaaaa acgggggggt tcttggtatt 2100
aattccagcg atggaattaa agctcctgag actggggtgg gacaaaatca aacaaaatag 2160
taacaacgaa ttgatatgaa cccattttgc tttgtactta tacaagacag gatagcatcc 2220
cataagaaga tccttttaaa ttggctttgg atatgattca tgatccccag ggcagaatta 2280
agacagatca aactaaagat attaagcata acaaacattt tgttcttgga gaaaatttca 2340
ttattattgg gttattgata taaggggaaa atgaagaaag aagggaaagt aggccgcaaa 2400
atatttcttt ttctttgaac tccctcaatc aaaaatcctt caattctcaa atgagaatag 2460
aaggaatcat accttacata caatatctta attgaattat atgtaatgat caataaattc 2520
atttttattc tacatctaca tgtgtaaaat catagcaaca accaattcaa tagatattgg 2580
ggctggaatt gacgaacaac caataccgat attagtgttt atcggtgtcg aatagaaata 2640
aaaatggggc gtggccaagt ggtaaggcaa cgggttttgg tcccgttact cggaggt 2697
<210> 45
<211> 2699
<212> DNA
<213> Machilus yunnanensis
<400> 45
gaagggattg atgaaacaaa tcaaaaaaaa attcaattta tttcgactat aaaaaagtct 60
ctttctaata ttagtaataa taaatcaaaa atttctggtg acccatattc cttttcacaa 120
gcatctgtat tttacaaatt atcacaaatc gaagctatca agaagtatca tttgagatct 180
ctacttcaat atcgcgaagc atatcttatt cttaaggata gaatcaggaa tttttttgga 240
acacgaagaa tatttgattc caaatcaagg cataaaaaac ttccgaattc tggaatgaat 300
gaatggaaaa actggttaag gggtcattat caatacaatt tatctcagga tttatcgata 360
atcttttttg ttgattcaag gaaaagttgt acattgactc tagaaatatt aaccgtacat 420
agaaagatat gtatgtatat tctttcgttg aaaaatgtca aaaaatagtg ccatttgcgt 480
atgaatatga atctgttact tcttcttttt gatatctgcc aaataggttt ctgcgattcc 540
gatcttttat caccacaact tgtatcgtta ggactaatat ttatatccgg agttagaaat 600
atttttcttt tgtcttttgc acccctttct atttgatttc tgattagggt tgttctatcg 660
ttttcctcct cccgctcttc aaaagaatcg atcaatttgt atgatcgtcg aggaatcttt 720
ttaccgattt cttctattcc aatagatttt ttttgaattg tttgattatt tggatcagtt 780
gtaattacat cgaatagaaa tttcaaacat tttgttttat tttctgaatc aaatcttctt 840
tgttcggata ttaaaacaag ctttttcaaa ttaagattaa aaccagttgt tgatttccta 900
gctaattcac tgatagaggt caagaaagga ccaatgtctg tcgataatga ttctccatta 960
aatatatcca ttttatgttc aagtttttgg taatcagtag gaagcatatc atgaatctta 1020
tttatccaaa ccattcctat ggaatcttct gtccccattc cacctaaaat cctaggaatt 1080
cgttgatggt tggaatagat tcgtagaccg ggacgactga tacgctttaa aatagttcta 1140
tatgttcctt tcctattcct tctatgtcgc agggttgaaa ccaaaaaata tttgttgttt 1200
tccctatgtt tcctaacatt ttcaataaac ccttcttgta gaagtatttt aacaatgttt 1260
tcggcgatat tagtagatgc tattcgaacc gttccttttt tatccatgtt agcatttctt 1320
atagaagtta ttatatcggc aatagtgtcc ctacccatga cgaactaaaa ttatgggtgc 1380
cttccagttt tgatacaatc aacatgttcc tttttttttt tcatctacta gtatttataa 1440
gacttcagga gctaatgaga ctattttagt gaaattcaac tgtctcaatt cccgcgcgat 1500
cgctccaaaa acgcgagttc cttttggatt tccttcttga tcaatgacaa ctgctgcatt 1560
gtcatcatat cgtattatca taccgttgtc gcgtttgagt tctttacatg tacgtacaat 1620
tacagctctg atcacttctg atctttcgag aggcatattg ggcactgctt ctttgattac 1680
agcaacaata acgtcaccca tatgagcata tcgttgatta ctagctccta tgatttgaat 1740
acacatcaat tctcgagccc cgctgttgtt cgctacattc aaaagggttt gggggtgaat 1800
catatcattt tttttttaac aaagatcaaa gtgaaattca aaaaaaaaaa ctgggtgggc 1860
cattcagcgt atgttcgccc cctcgcccat attcatattg aaggttagtt agtcatttgg 1920
ataaacttta tgtcccatat ctatgatatt tggattctca catgatgcat tctgagtcga 1980
aatgcgaaat aaaagagaag tctcgacctt ccctaaagta aatataaata aagatagggt 2040
agacaaaatg actaattcta tgtggatcct gaatcctaaa aaacgggggg gttcttggta 2100
ttaattccag cgatggaatt aaagctcctg agactggggt gggacaaaat caaacaaaat 2160
agtaacaacg aattgatatg aacccatttt gctttgtact tatacaagac aggatagcat 2220
cccataagaa gatcctttta aattggcttt ggatatgatt catgatcccc agggcagaat 2280
taagacagat caaactaaag atattaagca taacaaacat tttgttcttg gagaaaattt 2340
cattattatt gggttattga tataagggga aaatgaagaa agaagggaaa gtaggccgca 2400
aaatatttct ttttctttga actccctcaa tcaaaaatcc ttcaattctc aaatgagaat 2460
agaaggaatc ataccttaca tacaatatct taattgaatt atatgtaatg atcaataaat 2520
tcattttgat tctacatcta catgtgtaaa atcatagcaa caaccaattc aatagatatt 2580
ggggctggaa ttgacgaaca accaataccg atattagtgt ttatcggtgt cgaatagaaa 2640
taaaaatggg gcgtggccaa gtggtaaggc aacgggtttt ggtcccgtta ctcggaggt 2699
<210> 46
<211> 19
<212> DNA
<213> Artificial Sequence
<400> 46
gaccacctag cctgagata 19
<210> 47
<211> 19
<212> DNA
<213> Artificial Sequence
<400> 47
agaagggatt gatgaaaca 19
<210> 48
<211> 21
<212> DNA
<213> Artificial Sequence
<400> 48
tgatttgttt catcaatccc t 21
<210> 49
<211> 21
<212> DNA
<213> Artificial Sequence
<400> 49
cgatagaaca accctaatca g 21
<210> 50
<211> 19
<212> DNA
<213> Artificial Sequence
<400> 50
tttggctttt cttgaacga 19
<210> 51
<211> 20
<212> DNA
<213> Artificial Sequence
<400> 51
gatgactcaa tcacttcgac 20
<210> 52
<211> 19
<212> DNA
<213> Artificial Sequence
<400> 52
tcctttgcgg attaactca 19
<210> 53
<211> 20
<212> DNA
<213> Artificial Sequence
<400> 53
taggatggga acaaagatca 20
<210> 54
<211> 20
<212> DNA
<213> Artificial Sequence
<400> 54
aaggattcga acctccgagt 20
<210> 55
<211> 25
<212> DNA
<213> Artificial Sequence
<400> 55
agggcagaat taagacagat caaac 25
<210> 56
<211> 23
<212> DNA
<213> Artificial Sequence
<400> 56
ccaattcttt ctagtcgagc ttc 23
<210> 57
<211> 24
<212> DNA
<213> Artificial Sequence
<400> 57
gatcacgtta gtagattgtg tctc 24
<210> 58
<211> 23
<212> DNA
<213> Artificial Sequence
<400> 58
tgagacacaa tctactaacg tga 23
<210> 59
<211> 20
<212> DNA
<213> Artificial Sequence
<400> 59
ttttcctttg ccttttgcat 20
Claims (8)
1. A DNA barcode for identifying a plurality of woods in a genus machilus and a genus machilus;
the DNA barcode is a ycf1+ rps16-psbI + rps8-rpl16 combined barcode, and the ycf1+ rps16-psbI + rps8-rpl16 combined barcode is shown as SEQ ID No. 1-45;
the wood of the Phoebe bournei, phoebe Zhejiang Phoebe P.chekiangensis, bai Nan P.neurontha, phoebe leaf P.meiensis, phoebe P.sheareri, phoebe P.zhennan, rust Mao Runnan machinery balansae, phoebe M.leprophylla, phoebe M.pauhoi, phoebe M.thunbergii and Phoebe M.yunnanensis are respectively used as the wood of the Phoebe and Phoebe thunbergii.
2. A method for identifying a plurality of wood species in machilus and machilus based on the DNA barcode of claim 1, comprising the steps of:
s100, grinding a wood sample at the low temperature of-196 ℃ to prepare a wood powder sample;
s200, extracting a DNA sample in the wood powder sample;
s300, amplifying ycf1, rps16-psbI and rps8-rpl16 barcode sequences in a plastid genome sequence by using the DNA sample as a template through PCR to obtain an amplification product;
s400, sequencing the amplification products to obtain sequences of the ycf1, the rps16-psbI and the rps8-rpl16, and combining the sequences to obtain a combined barcode of the ycf1+ rps16-psbI + rps8-rpl 16;
s500, identifying various wood in the Nanmu and the Machilus by adopting the ycf1+ rps16-psbI + rps8-rpl16 combined bar code and based on a Taxon DNA method and a phylogenetic evolution tree method.
3. The method of claim 2, wherein the step S100 further comprises the steps of:
s101, cutting wood into short wood pieces by using a sterile scalpel, soaking the short wood pieces in sterile 50% ethanol for 1 hour, and performing air drying treatment to obtain an air-dried sample;
s102, adding the air-dried sample and crosslinked polyvinylpyrrolidone powder with the mass ratio of 0.5-1 into a freezing and grinding tube, and mixing and grinding at the low temperature of-196 ℃ to prepare grinding powder;
s103, sieving the ground powder by a sieve of 50-200 meshes to prepare the wood powder sample;
and S104, putting the wood flour sample into a sterilized centrifugal tube for subpackaging and storing.
4. The method for identifying a plurality of woods in the genus machilus and the genus machilus as claimed in claim 2, further comprising the step of, in step S200:
s201, adding a rinsing liquid into the wood flour sample, mixing for the first time, rinsing the secondary metabolites of xylem of Nana/Machilus of the wood flour sample by using the rinsing liquid, centrifuging for 10-15min at 3000-6000 g by using a centrifuge after uniformly mixing, and discarding supernatant liquid to obtain first rinsing powder;
s202, adding the rinsing liquid into the first rinsing powder, mixing for the second time, and repeating the centrifugation process in the step S201 to obtain second rinsing powder;
s203, extracting DNA from the second rinsing powder, and purifying the extracted DNA to obtain the DNA sample;
wherein the mixing time of the first mixing and the second mixing is 10min;
the rinsing liquid comprises: 100-300 mM Tris-HCl with pH 8.0, 10-30 mM Tris-HCl with pH 8.0, 1-4%w/v PVP-40,5% v/v glycerol, 5-10% w/vPEG8000 and 300-500 mM sorbitol;
before use, the rinsing liquid is added with 1-2%v/v beta-mercaptoethanol.
5. The method of claim 2, wherein in step S300,
the reaction system for PCR amplification is 0.5-3U DNA polymerase and 1.0-2.0 mM MgCl 2 200 mu M single dNTP, 0.1-5.0 mu M single primer, 0.1-1.5 mg/mL bovine serum albumin with pH of 7.0-8.0 and 10-1000 ng template DNA;
the reaction procedure for the PCR amplification is as follows: pre-denaturation at 94-96 deg.c for 0.5-10 min; denaturation at 94-96 deg.C for 0.05-2min, annealing at 40-65 deg.C for 0.5-2min, extension at 72 deg.C for 0.3-2 min, and circulation for 20-50 times; final extension at 72 deg.c for 2-15 min;
wherein, the positive and negative primers for amplifying the ycf1 sequence are shown in SEQ ID No.46-51, the positive and negative primers for amplifying the rps16-psbI sequence are shown in SEQ ID No.52-55, and the positive and negative primers for amplifying the rps8-rpl16 sequence are shown in SEQ ID No. 56-59.
6. The method of claim 2, wherein in step S500;
the Taxon DNA method is based on best close match index judgment;
the phylogenetic evolutionary tree method is based on adjacency method identification.
7. The method of claim 2, wherein the index is based on the combined use of 4 indices of nucleotide polymorphism Pi, DNA fragment acquisition success rate, fragment length, and discrimination ability.
8. The method of claim 7, wherein the nucleotide polymorphism Pi index is based on a plastid genome-wide sequence alignment and Pi > 0.003;
the success rate of obtaining the DNA fragments is more than or equal to 70 percent;
the length of the fragment is 150-500 bp;
the discriminative power is discriminated based on the Taxon DNA method and the phylogenetic tree method.
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