CN114540250A - Fermentation inoculant for rapeseed meal and preparation method thereof - Google Patents

Fermentation inoculant for rapeseed meal and preparation method thereof Download PDF

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CN114540250A
CN114540250A CN202210309837.9A CN202210309837A CN114540250A CN 114540250 A CN114540250 A CN 114540250A CN 202210309837 A CN202210309837 A CN 202210309837A CN 114540250 A CN114540250 A CN 114540250A
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rapeseed meal
parts
fermentation
bag
culture medium
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赵琦锴
王景峰
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Leshan Hengfeng Huabang Biotechnology Co ltd
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Leshan Hengfeng Huabang Biotechnology Co ltd
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    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/24—Compounds of alkaline earth metals, e.g. magnesium
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/30—Oligoelements
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
    • A23L5/27—Removal of unwanted matter, e.g. deodorisation or detoxification by chemical treatment, by adsorption or by absorption
    • A23L5/276—Treatment with inorganic compounds
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
    • A23L5/28—Removal of unwanted matter, e.g. deodorisation or detoxification using microorganisms
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
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    • C12N1/18—Baker's yeast; Brewer's yeast
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/14—Hydrolases (3)
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    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
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    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/11—Lactobacillus
    • A23V2400/169—Plantarum
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Abstract

The invention provides a fermentation microbial inoculum for rapeseed meal, and relates to the field of microbial fermentation. The fermentation inoculant for rapeseed meal comprises the following components: the preparation method of the fermentation inoculant for the rapeseed meal comprises the following steps of (1) preparing a fermentation inoculant for the rapeseed meal, wherein the fermentation inoculant comprises the following steps: the method comprises the following steps: step one, fungus cultivation; step two, extracting strains; step three, configuration is carried out; and step four, packaging. Through adopting mineral substance and microbial srain together to participate in the fermentation, make inside harmful substance handle comparatively rapidly, reduce the time of soybean meal fermentation by a wide margin, through using mineral substance processing earlier, make inside partial harmful substance react, then get rid of remaining harmful substance through the microbial srain, not only get rid of comparatively cleanly, can effectively increase the protein content of rapeseed meal moreover.

Description

Fermentation inoculant for rapeseed meal and preparation method thereof
Technical Field
The invention relates to the technical field of microbial fermentation, in particular to a fermentation microbial inoculum for rapeseed meal and a preparation method thereof.
Background
The content of crude protein in the rapeseed dregs should be above 32%, and the content of crude fiber should be below 12%. Rape is one of the main oil crops in China, and 95 percent of rapeseeds in China are used for producing edible oil. The common oil pressing process of rapeseed includes two processes of power rotary pressing and prepressing leaching, wherein the byproduct of the former process is rapeseed cake, and the byproduct of the latter process is rapeseed dregs.
The fermented rapeseed meal is a protein raw material product for feeding, which is prepared by taking rapeseed meal as a main raw material, taking corn and bran as auxiliary raw materials, adding feeding microorganisms and feeding enzyme preparations for solid state fermentation, and drying. Through the microbial fermentation and the action of enzyme, the rapeseed meal is effectively detoxified, the protein content of the rapeseed meal is improved, the protein quality in the rapeseed meal is improved, and the protein digestibility and the nutritional value of the rapeseed meal are improved, so that the use proportion of the rapeseed meal in a feed formula is increased, and the shortage of feed protein resources in China is made up.
The existing fermentation inoculum has longer fermentation time on rapeseed meal, and the content of protein generated by fermentation is less, so that the protein digestion rate is lower.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides a fermentation inoculant for rapeseed meal and a preparation method thereof, and solves the problems of long fermentation time and low protein digestibility.
(II) technical scheme
In order to achieve the purpose, the invention is realized by the following technical scheme: a fermentation inoculum for rapeseed meal comprises the following components: bacillus amyloliquefaciens, lactic acid bacteria, cellulose, saccharomyces cerevisiae, mineral substances and lactobacillus plantarum.
Preferably, the fermentation inoculant comprises the following components in parts by mass: 1-50 parts of bacillus amyloliquefaciens, 1-20 parts of lactic acid bacteria, 1-20 parts of cellulase, 1-50 parts of saccharomyces cerevisiae, 30-80 parts of minerals and 1-20 parts of lactobacillus plantarum.
Preferably, the total viable count of the bacillus amyloliquefaciens is 1-20 hundred million CFU/g, the total viable count of the lactic acid bacteria is 100-300 hundred million CFU/g, the total viable count of the saccharomyces cerevisiae is 50-100 hundred million CFU/g, and the total viable count of the lactobacillus plantarum is 80-200 hundred million CFU/g.
Preferably, the minerals comprise: the corrosion inhibitor comprises the following components in parts by weight: 1-5 parts of sodium hydroxide, 6-10 parts of calcium carbide, 3-6 parts of copper sulfate, 4-7 parts of zinc sulfate and 10-20 parts of sodium chloride.
A preparation method of a fermentation inoculant for rapeseed meal comprises the following steps:
step one, fungus cultivation: placing bacillus amyloliquefaciens, lactic acid bacteria, saccharomyces cerevisiae and lactobacillus plantarum in a proper culture medium to enable the total number of living bacteria in the bacillus amyloliquefaciens to reach the standard;
step two, strain extraction: separating the strains which are cultured to reach the standard by a centrifugal machine, collecting the strains, and placing the strains in a strain bag for weighing;
step three, configuration: preparing bacillus amyloliquefaciens, lactic acid bacteria, cellulose, saccharomyces cerevisiae and lactobacillus plantarum according to corresponding mass ratio, and filling into a bag A;
step four, packaging: mixing the mineral substances according to the corresponding proportion, weighing according to the mass ratio in the third step, filling into a bag B, and packaging the bag A and the bag B together.
Preferably, the culture medium adopted by the bacillus amyloliquefaciens is a broth culture medium, the culture medium adopted by the saccharomyces cerevisiae is a potato-glucose culture medium, and the culture medium adopted by the lactic acid bacteria and the lactobacillus plantarum is a lactic acid bacteria culture medium.
A use method of a fermentation inoculant for rapeseed meal comprises the following steps:
step one, rapeseed meal pretreatment: scattering the extruded rapeseed meal, and removing internal bacteria through sterilization treatment;
step two, chemical reaction: mixing the mineral substances in the bag B into the sterilized rapeseed meal, and uniformly mixing for 4-6 hours;
step three, mixed bacteria reaction: and (3) mixing the microbial inoculum in the bag A into the reacted rapeseed meal, then uniformly mixing, and reacting for 12-16 hours.
Preferably, a stirrer is adopted in the mixing of the second step and the third step.
(III) advantageous effects
The invention provides a fermentation inoculant for rapeseed meal and a preparation method thereof. The method has the following beneficial effects:
1. according to the invention, mineral substances and microbial bacteria are adopted to jointly participate in fermentation, so that the harmful substances in the soybean meal are treated more quickly, and the fermentation time of the soybean meal is greatly reduced.
2. According to the method, the minerals are used for treatment, so that partial harmful substances in the rapeseed meal react, and then the residual harmful substances are removed through the microbial bacteria, so that the removal is clean, and the protein content of the rapeseed meal can be effectively increased.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example (b):
the embodiment of the invention provides a fermentation inoculant for rapeseed meal, which comprises the following components: bacillus amyloliquefaciens, lactic acid bacteria, cellulose, saccharomyces cerevisiae, mineral substances and lactobacillus plantarum.
The fermentation inoculum comprises the following components in parts by mass: 1-50 parts of bacillus amyloliquefaciens, 1-20 parts of lactic acid bacteria, 1-20 parts of cellulase, 1-50 parts of saccharomyces cerevisiae, 30-80 parts of mineral substances and 1-20 parts of lactobacillus plantarum.
The total viable count of bacillus amyloliquefaciens is 1-20 hundred million CFU/g, the total viable count of lactobacillus is 100-300 hundred million CFU/g, the total viable count of saccharomyces cerevisiae is 50-100 hundred million CFU/g, the total viable count of lactobacillus plantarum is 80-200 hundred million CFU/g, and the minerals comprise: the corrosion-resistant steel plate comprises sodium hydroxide, calcium carbide, copper sulfate, zinc sulfate and sodium chloride, wherein the sodium hydroxide, the calcium carbide, the copper sulfate, the zinc sulfate and the sodium chloride are as follows in parts by weight: 1-5 parts of sodium hydroxide, 6-10 parts of calcium carbide, 3-6 parts of copper sulfate, 4-7 parts of zinc sulfate and 10-20 parts of sodium chloride.
A preparation method of a fermentation inoculant for rapeseed meal comprises the following steps:
step one, fungus cultivation: placing bacillus amyloliquefaciens, lactic acid bacteria, saccharomyces cerevisiae and lactobacillus plantarum in a proper culture medium to enable the total number of living bacteria in the bacillus amyloliquefaciens to reach the standard;
step two, strain extraction: separating the strains which are cultured to reach the standard by a centrifugal machine, collecting the strains, and placing the strains in a strain bag for weighing;
step three, configuration: preparing bacillus amyloliquefaciens, lactic acid bacteria, cellulose, saccharomyces cerevisiae and lactobacillus plantarum according to corresponding mass ratio, and filling into a bag A;
step four, packaging: mixing the minerals according to the corresponding proportion, weighing according to the mass ratio in the step three, filling into a bag B, and packaging the bag A and the bag B together.
The culture medium adopted by the bacillus amyloliquefaciens is a broth culture medium, the culture medium adopted by the saccharomyces cerevisiae is a potato-glucose culture medium, and the culture medium adopted by the lactobacillus and the lactobacillus plantarum is a lactobacillus culture medium.
A use method of a fermentation inoculant for rapeseed meal comprises the following steps:
step one, rapeseed meal pretreatment: scattering the extruded rapeseed meal, and removing internal bacteria through sterilization treatment;
step two, chemical reaction: mixing the mineral substances in the bag B into the sterilized rapeseed meal, and uniformly mixing for 4-6 hours;
step three, mixed bacteria reaction: and (3) mixing the microbial inoculum in the bag A into the reacted rapeseed meal, then uniformly mixing, and reacting for 12-16 hours.
And a stirrer is adopted when the step two and the step three are mixed.
Comparative example one:
the difference from the embodiment is as follows: no mineral component is added.
Comparative example two:
the difference of the other embodiments is as follows: only mineral components are added, and saccharomyces cerevisiae is added.
The comparative data of the examples and comparative examples one and two are shown in table one:
table one example compares the data with the comparative example
Total time of treatment (H) Protein digestibility (%)
Examples 16～22 85～88
Comparative example 1 28～32 78～83
Comparative example No. two 24～36 72～75
As can be seen from the data in Table I, the method of the present invention can effectively reduce the fermentation time and simultaneously improve the internal protein digestibility.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (8)

1. A fermentation inoculant for rapeseed meal, which is characterized in that: comprises the following components: bacillus amyloliquefaciens, lactic acid bacteria, cellulose, saccharomyces cerevisiae, mineral substances and lactobacillus plantarum.
2. The fermentation inoculant for rapeseed meal according to claim 1, which is characterized in that: the fermentation inoculant comprises the following components in parts by mass: 1-50 parts of bacillus amyloliquefaciens, 1-20 parts of lactic acid bacteria, 1-20 parts of cellulase, 1-50 parts of saccharomyces cerevisiae, 30-80 parts of minerals and 1-20 parts of lactobacillus plantarum.
3. The fermentation inoculant for rapeseed meal according to claim 1, which is characterized in that: the total viable count of the bacillus amyloliquefaciens is 1-20 hundred million CFU/g, the total viable count of the lactic acid bacteria is 100-300 hundred million CFU/g, the total viable count of the saccharomyces cerevisiae is 50-100 hundred million CFU/g, and the total viable count of the lactobacillus plantarum is 80-200 hundred million CFU/g.
4. The fermentation inoculant for rapeseed meal according to claim 1, which is characterized in that: the minerals include: the corrosion-resistant steel plate comprises sodium hydroxide, calcium carbide, copper sulfate, zinc sulfate and sodium chloride, wherein the sodium hydroxide, the calcium carbide, the copper sulfate, the zinc sulfate and the sodium chloride are as follows in parts by weight: 1-5 parts of sodium hydroxide, 6-10 parts of calcium carbide, 3-6 parts of copper sulfate, 4-7 parts of zinc sulfate and 10-20 parts of sodium chloride.
5. The fermentation inoculant for rapeseed meal according to claim 1, which is characterized in that: the preparation method comprises the following steps:
step one, fungus cultivation: placing bacillus amyloliquefaciens, lactic acid bacteria, saccharomyces cerevisiae and lactobacillus plantarum in a proper culture medium to enable the total number of living bacteria in the bacillus amyloliquefaciens to reach the standard;
step two, strain extraction: separating the strains which are cultured to reach the standard by a centrifugal machine, collecting the strains, and placing the strains in a strain bag for weighing;
step three, configuration: preparing bacillus amyloliquefaciens, lactic acid bacteria, cellulose, saccharomyces cerevisiae and lactobacillus plantarum according to corresponding mass ratio, and filling into a bag A;
step four, packaging: mixing the minerals according to the corresponding proportion, weighing according to the mass ratio in the step three, filling into a bag B, and packaging the bag A and the bag B together.
6. The fermentation inoculant for rapeseed meal according to claim 5, wherein: the culture medium adopted by the bacillus amyloliquefaciens is a broth culture medium, the culture medium adopted by the saccharomyces cerevisiae is a potato-glucose culture medium, and the culture medium adopted by the lactic acid bacteria and the lactobacillus plantarum is a lactic acid bacteria culture medium.
7. The fermentation inoculant for rapeseed meal according to claim 5, wherein: the method comprises the following use methods:
step one, rapeseed meal pretreatment: scattering the extruded rapeseed meal, and removing internal bacteria through sterilization treatment;
step two, chemical reaction: mixing the mineral substances in the bag B into the sterilized rapeseed meal, and uniformly mixing for 4-6 hours;
step three, mixed bacteria reaction: and (3) mixing the microbial inoculum in the bag A into the reacted rapeseed meal, then uniformly mixing, and reacting for 12-16 hours.
8. The fermentation inoculum for rapeseed meal according to claim 7, characterized in that: and a stirrer is adopted when the step two and the step three are mixed.
CN202210309837.9A 2022-03-28 2022-03-28 Fermentation inoculant for rapeseed meal and preparation method thereof Pending CN114540250A (en)

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KR20200087388A (en) * 2019-01-10 2020-07-21 정채규 Animal feed additive using agricultural wastes and process for preparing the same
CN112980707A (en) * 2019-12-02 2021-06-18 安琪酵母股份有限公司 Microbial inoculum for removing anti-nutritional factors in soybean meal and preparation process of fermented soybean meal
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CN1044386A (en) * 1990-02-28 1990-08-08 中国科学院成都有机化学研究所 A kind of rapeseed dregs chemical detoxication
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KR20200087388A (en) * 2019-01-10 2020-07-21 정채규 Animal feed additive using agricultural wastes and process for preparing the same
CN112980707A (en) * 2019-12-02 2021-06-18 安琪酵母股份有限公司 Microbial inoculum for removing anti-nutritional factors in soybean meal and preparation process of fermented soybean meal
CN113073056A (en) * 2020-01-06 2021-07-06 安琪酵母股份有限公司 Fermentation inoculant for rapeseed meal and application thereof

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