CN114540210A - Preparation and application of composite microbial agent for environment-friendly ecological toilet - Google Patents

Preparation and application of composite microbial agent for environment-friendly ecological toilet Download PDF

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CN114540210A
CN114540210A CN202011333786.0A CN202011333786A CN114540210A CN 114540210 A CN114540210 A CN 114540210A CN 202011333786 A CN202011333786 A CN 202011333786A CN 114540210 A CN114540210 A CN 114540210A
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ecological toilet
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闫志英
王茄灵
许力山
刘杨
姬高升
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Chengdu Institute of Biology of CAS
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Abstract

The invention relates to the technical field of microorganisms, and particularly relates to preparation and application of a composite microbial inoculum for an environment-friendly ecological toilet. The specific technical scheme is as follows: the environment-friendly composite microbial agent for the ecological toilet comprises pediococcus acidilactici and bacillus belvesii, can grow well in ecological side sitting excrement, occupies favorable ecological niches, inhibits the growth and the reproduction of odor-producing microorganisms, reduces odor release strength, and realizes odor in-situ source control. Meanwhile, the decomposition and degradation of the excrement are promoted.

Description

Preparation and application of composite microbial agent for environment-friendly ecological toilet
Technical Field
The invention relates to the technical field of microorganisms, and particularly relates to preparation and application of a composite microbial inoculum for an environment-friendly ecological toilet.
Background
Because of the large population of China and the large amount of human excrement and urine generated every day, how to solve the problems of excrement and foul smell is a common problem faced by big families. And most of the residents in cities and towns in China all adopt water to flush toilets, and human excrement and urine enter a sewage treatment plant for treatment through a municipal domestic sewage discharge pipe network. However, the water-flushing toilet not only increases the amount of the excrement, but also increases the difficulty of harmless treatment after the excrement is diluted, thereby being not beneficial to the reutilization of the excrement resource. In some old cities and rural areas, especially in alpine and desert regions in China, water resources are extremely deficient, dry toilets still exist, the difficulty of controlling excrement is high, the sealing performance is poor, and serious environmental pollution is caused; and some outdoor septic tanks have outstanding problems of odor and mosquitoes and flies, and the life quality of people is greatly reduced. Although the rural areas have the condition of manure utilization, if the manure carries virus and germs and is not subjected to harmless treatment, people who contact the manure are infected, and secondary pollution to the environment is caused. Therefore, the implementation of the control and management of the odor pollution of the excrement and the development of the research on removing the malodorous gas have important significance.
At present, the microbial agents which are applied, such as the invention patent with the Chinese patent application publication number of CN110157644A, disclose that the combination of Bacillus flexus and Bacillus megaterium can fix sulfur and nitrogen and effectively remove hydrogen sulfide and ammonia gas with odor and toxicity; but the biological diversity index is lower, the components are single, the effect is difficult to be exerted in a short time, and the deodorization efficiency is not high. For example, the invention patent with Chinese patent application publication No. CN106967640A discloses that the deodorization effect better than that of a single strain can be achieved by the cooperation of pseudomonas and bacillus and the comprehensive treatment of malodor; however, the contained pseudomonas and other conditional pathogenic bacteria have potential risks to human bodies and the environment. Therefore, the preparation of the efficient compound microbial deodorant bacterial agent needs to ensure the deodorant effect, and the microorganisms can quickly proliferate in the odor source to form a beneficial flora system, so that the odor intensity released by excrement is effectively reduced, and the safety of bacterial strains is also taken into consideration.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a high-efficiency microbial composite deodorant microbial inoculum capable of degrading excrement and inhibiting odor sources and a preparation method thereof.
In order to achieve the purpose, the invention is realized by the following technical scheme:
the invention discloses a composite microbial inoculum for an environment-friendly ecological toilet, which comprises pediococcus acidilactici and bacillus beiLeisi.
Preferably, the pediococcus acidilactici is preserved in the China general microbiological culture Collection center with the preservation number of CGMCC NO: 19770 and/or the Bacillus belmentioned is preserved in China general microbiological culture Collection center with the preservation number of CGMCC NO: 19769.
preferably, the concentration of the bacterial liquid of the pediococcus acidilactici and the bacillus beiLeisi is 109CFU/mL。
Preferably, in the composite microbial inoculum, the volume ratio of the pediococcus acidilactici to the bacillus beiLeisi bacterial liquid is 0.5-2: 0.5-2.
Preferably, the complex microbial inoculum also comprises microbial fruit ferment.
Preferably, the microbial fruit ferment is prepared by inoculating lactobacillus and aroma-producing yeast into fruits and brown sugar, fermenting and filtering.
Correspondingly, the preparation method of the composite microbial inoculum for the environment-friendly ecological toilet comprises the steps of activating pediococcus acidilactici and bacillus belgii, respectively inoculating the activated pediococcus acidilactici and bacillus belgii into liquid culture media, and culturing until the concentrations of bacterial liquids are respectively 109And CFU/mL, mixing the two bacterial liquids to obtain a liquid microbial inoculum, and finally adding microbial fruit enzyme according to the proportion of 10-15% to v/v in the liquid microbial inoculum to obtain the liquid composite microbial inoculum.
Preferably, adding an adsorbent into the liquid composite microbial inoculum, uniformly stirring, and drying to obtain a dried composite microbial inoculum; and then adding an accelerant into the dried complex microbial inoculum, and uniformly stirring to obtain the solid complex microbial inoculum.
Preferably, the adsorbent is prepared by mixing bran, rice bran and vinasse, and the accelerant is yeast powder; in the liquid composite bacterial agent, the number of viable bacteria is more than or equal to 109CFU/mL, wherein the addition amount of the adsorbent is 0.5-2 kg/L; in the drying composite microbial inoculum, the addition amount of the accelerant is 4-7 g/kg.
Correspondingly, the composite microbial inoculum is applied to the deodorization treatment of the excrement in the ecological toilet.
The invention has the following beneficial effects:
1. the microorganisms in the composite microbial inoculum provided by the invention can grow well in the feces of the ecological toilet, occupy favorable ecological niches, inhibit the growth and reproduction of odor-producing microorganisms, reduce the odor release strength and realize the control of the odor in-situ source. Meanwhile, the decomposition and degradation of the excrement are promoted, and the volume of the excrement is reduced. The requirements of 'three-zero and three-change' of zero pollution, zero infection, zero waste discharge, resource, harmlessness and reduction are met.
2. The composite microbial inoculum provided by the invention is composed of beneficial microorganisms such as lactobacillus, saccharomycetes and bacillus, has extremely high activity, and can be rapidly proliferated in an odor source to form a high-efficiency stable probiotic system. The lactic acid bacteria have super-strong acid-producing capability, the yeast secretes physiological active substances, promotes the fermentation operation of excrement, constructs a toilet environment full of beneficial microorganisms, effectively improves the sanitary condition of the toilet, reduces the breeding of germs, and ensures the safety, comfort and sanitation of toilet.
3. The invention realizes effective combination of strains, and the combined microbial inoculum has obviously improved deodorization effect compared with single strain. The lactobacillus is used as the leading bacteria, beneficial bacteria coexist mutually and quickly grow into dominant bacteria, and the dominant bacteria play a role in inhibiting odor-producing harmful bacteria; meanwhile, the yeast can also generate fragrance and can cover odor.
4. The composite microbial inoculum provided by the invention has wide application range, can be normally used in high-cold and arid places such as inner Mongolia autonomous regions, has wide growth temperature, can resist high temperature and low temperature, and has obvious degradation and deodorization effects. The bacterial strains contained in the complex microbial inoculum are obtained by screening from landfill leachate and vinasse, and do not cause harm to the ecological environment and livestock and poultry animals.
Detailed Description
The following will clearly and completely describe the technical solutions in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Unless otherwise indicated, the technical means used in the examples are conventional means well known to those skilled in the art.
1. The invention provides a composite microbial inoculum for an environment-friendly ecological toilet, which comprises pediococcus acidilactici and bacillus beiLeisi. The concentration of the bacterial liquid after fermentation of pediococcus acidilactici and bacillus belgii is 109CFU/mL. In the composite microbial inoculum, the volume ratio of the pediococcus acidilactici to the bacillus belgii is 0.5-2: 0.5-2, and preferably 1: 1.
Wherein, Pediococcus acidilactici (with the number of WP) is preserved in the China general microbiological culture Collection center of the Committee for culture Collection of microorganisms with the preservation number of CGMCC NO. 19770; and/or the Bacillus velezensis (WB) is preserved in the common microorganism center of China Committee for culture Collection of microorganisms with the preservation number of CGMCC NO. 19769. Pediococcus acidilactici and Bacillus belgii are both preserved in China general microbiological culture Collection center (CGMCC) at 2020, 05 and 06 days, and are addressed to Beijing, Tokyo, Yang-oriented region, Beichen Xilu No.1 Hospital.
The results of physiological and biochemical identification of Pediococcus acidilactici WP are shown in Table 1 below, and the results of physiological and biochemical identification of Bacillus belgii WB are shown in Table 2 below.
TABLE 1 physiological and biochemical identification results of Pediococcus acidilactici WP
Figure BDA0002796570030000041
TABLE 2 physiological and biochemical identification results of Bacillus belgii WB
Figure BDA0002796570030000042
Further, in the complex microbial inoculum, besides pediococcus acidilactici and bacillus belgii, microbial fruit enzymes are also included. The microbial fruit ferment is prepared by inoculating lactobacillus and aroma-producing yeast into fruit and brown sugar, fermenting, and filtering. The addition amount of the microbial fruit enzyme accounts for 10-15% (v/v) of the mixed bacterium liquid of pediococcus acidilactici and bacillus beiLeisi.
The specific preparation process of the microbial fruit enzyme comprises the following steps: cutting fruits into pieces, putting the cut fruits and water into a fermentation barrel according to a certain proportion, wherein the sample loading amount is 80-85% of the volume of the fermentation barrel, and simultaneously adding melted brown sugar; inoculating beneficial microorganism (Lactobacillus plantarum and aroma-producing yeast) bacterial liquid into the raw materials in the fermentation barrel, uniformly stirring, and then paving a plastic film to prevent the enzyme from losing. Fermenting for 3 months, and filtering to obtain ferment filtrate. Wherein the addition amount of the beneficial microorganism bacterial liquid is 0.006-0.7% of the weight of the mixture of the fruits and the water, and the viable bacteria amount is (0.5-10) × 108CFU/mL。
Wherein the mass ratio of the water to the fruits to the brown sugar is 10:3:1, and the fruits are selected from fruits which are not easy to rot and have fruit fragrance, such as apples, pears, orange peels, shaddock peels and the like, and are cut into pieces.
2. The invention also provides a preparation method of the complex microbial inoculum for the environment-friendly ecological toilet, and in the process of preparing the complex microbial inoculum, the used culture medium is as follows:
MRS solid culture medium subjected to high-temperature sterilization treatment (121 ℃, 30min) is quantitatively prepared, and the formula is as follows: 10g/L of peptone, 10g/L of beef extract, 5g/L of yeast extract, 5g/L of glucose, 5g/L of sodium acetate, 2g/L of diammonium citrate, 801 g/L of tween, 0.58g/L of magnesium sulfate, 0.05g/L of manganese sulfate, 2g/L of dipotassium hydrogen phosphate, 15-20g/L of agar and pH 6.2.
② beef extract peptone solid medium subjected to high-temperature sterilization treatment (121 ℃, 30min) is quantified for later use, and the formula is as follows: 3g/L of beef extract, 10g/L of peptone, 5g/L of NaCl, 15-20g/L of agar and 7.0-7.2 of pH value.
And thirdly, quantifying the MRS liquid culture medium subjected to high-temperature sterilization treatment (115 ℃ and 30min) for later use, wherein the MRS solid culture medium is prepared without adding agar.
And fourthly, quantitatively preparing the beef extract peptone liquid culture medium subjected to high-temperature sterilization treatment (at the temperature of 121 ℃ for 20min) for later use, wherein the formula is that agar is not added into the beef extract peptone solid culture medium.
The preparation process of the composite microbial inoculum comprises the following steps:
(1) inoculating Pediococcus acidilactici WP into MRS solid slant culture for activation and standby application, and inoculating Bacillus belgii WB into beef extract peptone solid slant culture medium for activation and standby application;
(2) inoculating activated pediococcus acidilactici WP into an MRS liquid culture medium, inoculating Bacillus belgii WB into a beef extract peptone liquid culture medium, and performing shake culture for 24h at 30 ℃ at 160 r/min;
(3) counting and adjusting cultured Pediococcus acidilactici WP bacterial liquid and Bacillus belgii WB bacterial liquid respectively until the bacterial liquid concentration of each bacterial strain is 109CFU/mL, mixing the two bacterial liquids according to the volume ratio of 1:1 to form a mixed bacterial liquid, and adding 10-15% (v/v) of the prepared enzyme filtrate to finally obtain the liquid composite microbial inoculum. In the liquid composite bacterial agent, the number of viable bacteria is more than or equal to 109CFU/mL, pH 5-7; suspension, brown yellow in color.
Further, the prepared liquid composite microbial inoculum can be prepared into a solid composite microbial inoculum, and the specific preparation process comprises the following steps: adding an adsorbent into the prepared liquid composite microbial inoculum, uniformly stirring, and then drying and culturing for 20-24 h in a constant-temperature incubator at 40 ℃ until the water content is 15-20%, thus obtaining a dried composite microbial inoculum; and then, adding an accelerant into the dried complex microbial inoculum, uniformly stirring to obtain a solid complex microbial inoculum, quantitatively packaging and storing at a low temperature (4-10 ℃). In the solid composite microbial inoculum, the viable count is more than or equal to 109CFU/mL, dry particles, milky surface.
The adsorbent is prepared by mixing sterilized bran, rice bran and vinasse according to the volume ratio of 2-4: 1-3 (preferably 2:2:1), and the addition amount of the adsorbent in a liquid composite microbial agent is 0.5-2 kg/L, preferably 1 kg/L; the accelerant is yeast powder, and the addition amount of the accelerant in the dried composite microbial inoculum is 4-7 g/kg, preferably 5 g/kg.
3. The invention also discloses application of the composite microbial inoculum to deodorization treatment of feces in the environment-friendly ecological toilet, which is mainly used for deodorizing the environment-friendly ecological toilet and decomposing and degrading the feces, and simultaneously reducing mosquitoes and flies and improving sanitary conditions of the toilet. The specific application method comprises the following steps: after the excrement enters a fermentation tank in the closestool, the filler, the solid compound microbial inoculum and the excrement are mixed by rotating a spiral stirrer in the fermentation tank. Under the state of constant temperature (35 ℃), the microorganisms in the composite microbial inoculum decompose the solid excrement into carbon dioxide, water and a small amount of excrement residues; and deodorizing the urine by using a liquid composite microbial inoculum, and specifically adding the liquid composite microbial inoculum according to 1-2% of the urine. After the solid excrement and urine are treated, the solid excrement and urine can be made into farmyard manure, so that the harmless utilization of manure resources is realized. Wherein, the filler comprises sawdust, rice hull and straw powder, and the specific proportion is prepared conventionally according to actual conditions. It should be noted that: the prepared composite microbial inoculum is matched with an ecological closestool (toilet bowl) for use, generally, 400-600 g of microbial inoculum is added into one closestool (toilet bowl), 3-6 kg of filler is added, the operation is continued, and the composite microbial inoculum is added after the material is changed for half a year or one year. Because the excrement is accompanied with urine, the added microbial inoculum generally adopts a solid compound microbial inoculum.
The invention is further illustrated below with reference to specific examples.
Example 1
1. Adding 10mL of liquid composite microbial inoculum into a beaker (1L) filled with 400g of fresh pig manure, sealing the beaker by a preservative film, respectively adding Pediococcus acidilactici WP bacterial liquid and Bacillus beijerinckii WB bacterial liquid into the beaker (1L) filled with 400g of fresh pig manure according to the method, and adding only one bacterial liquid into each beaker. At the same time, the bacteria-free agent is used asThe control group is provided with three replicates in each group of experiments, and is cultured in a constant temperature box at 35 ℃ and stirred regularly every day; meanwhile, a plurality of comparison groups are arranged by using different strains, and the specific reference is shown in the lower comparison example. After 7d, samples were taken for volatile solids VS, odor intensity values, NH3Concentration and H2The concentration of S. The degradation performance of the microbial inoculum is measured according to the change condition of VS, and the odor degree and NH3Concentration and H2And the deodorizing effect of the microbial inoculum is measured according to the change condition of the S concentration. The deodorizing effect of the microbial inoculum is measured by the change condition. The results are shown in Table 3 below. The results show that: the combined microbial inoculum has obviously improved deodorization effect compared with single bacterium.
TABLE 3 degradation and deodorization effects of the microbial inoculum
Figure BDA0002796570030000071
2. On the basis of the above experiment, the experiment was continued. The method specifically comprises the following steps: according to the above experiment, after 7d sampling test, stirring was continued periodically every day, and sampling test was performed at 14d, 21d and 30d, respectively. Specific results are shown in table 4 below. The control group showed that the feces of the control group emitted VS, odor intensity value, NH with the increase of time3Concentration and H2The S concentration also increased to a stable level.
TABLE 4 degradation and deodorization effects of the bacterial agents at different time periods
Figure BDA0002796570030000081
The results from table 4 above show that: with the extension of the degradation time, the content of residual organic solids in the culture tends to decrease, and the VS value decreases with the extension of the time, which indicates that the tested strains have the degradation effect on the solids in the excrement. The fresh excrement culture basically reaches no odor and no smell after a period of strong odor, which shows that the composite microbial inoculum has strong deodorizing effect on the odor generated in the excrement degradation process.
3. When VS, odor intensity, NH in feces3Concentration and H2And when the concentration of S reaches the maximum value or is in a stable stage, in order to verify the degradation and deodorization effects of the composite microbial inoculum on the substances, after the step 2 and the experiment for 30d, the composite microbial inoculum is added into excrement of a control group, six groups are arranged in the control group, each three groups are an experiment, and 10mL of liquid composite microbial inoculum and 10g of solid composite microbial inoculum are respectively added. Without stirring, samples were taken every 3 days to determine volatile solids VS, odor intensity values, NH3Concentration and H2The concentration of S. Specific results are shown in table 5 below. By combining the table 4 and the table 5, it can be seen that the complex microbial inoculum disclosed by the invention is used for treating whether the feces generate odor and VS; and the composite microbial inoculum is used for treating the natural plant material under the condition of stable VS and odor, so that the VS and the odor can be effectively degraded and deodorized. The solid complex microbial inoculum has larger contact surface of odor and the solid complex microbial inoculum due to larger surface area. Therefore, the solid complex microbial inoculum has good treatment effect on odor. However, the VS can be directly contacted with the liquid complex microbial inoculum, so that the liquid complex microbial inoculum has a good treatment effect on the VS.
TABLE 5 degradation deodorizing effect of the bacterial agent at high concentration of malodor (reduction/%)
Figure BDA0002796570030000091
Comparative example
1. Comparative experiments were performed using other species, as in the experiment in step 1 of example 1. The combinations of the different species are shown in table a below. The concentration of the bacterial liquid of each strain is 109CFU/mL, the volume ratio of the bacterial liquid of two strains in each group is 1:1, and the group 1 is 1:1:1: 1. The amount of the bacterial suspension prepared for each strain was the same as that of example 1.
TABLE a compounding of different strains
Figure BDA0002796570030000092
Figure BDA0002796570030000101
2. After experiments 7d, 14d, 21d, 30d, samples were taken for volatile solids VS, odor intensity values, NH3Concentration and H2The concentration of S. Specific results are shown in table b below.
TABLE b deodorant Effect of different strains (% reduction)
Figure BDA0002796570030000102
Figure BDA0002796570030000111
3. According to table b, the deodorizing effect of the bacteria solutions at different volume ratios is shown in table c below. For economy, Table c shows only one deodorizing effect after 30 d. The ratios in table c are in the order from left to right in table a.
TABLE c deodorant Effect of strains of different volume ratios (% reduction)
Figure BDA0002796570030000112
Figure BDA0002796570030000121
Example 2
Selecting four ecological toilets in a Chengdu Qinglong lake wetland park, adding 5kg of filler and 500g of solid compound microbial agent (after activation), starting a device, and putting into use; compared with other ecological toilets without microbial inoculum. Samples were taken after 7d, 14d, 21d and 30d, respectively, to determine the volatile solids VS, odor intensity values, NH3Concentration and H2And (4) taking the average value of the S concentration. Evaluating the degradation performance of the microbial inoculum according to the change condition of VS, and according to odor degree and NH3Concentration and H2And evaluating the deodorization effect of the microbial inoculum according to the change condition of the S concentration. Measuring knotThe results are shown in Table 6 below.
TABLE 6 degradation and deodorization effects of the microbial inoculum
Figure BDA0002796570030000122
Example 3
Installing four novel toilet bowl devices of ecological toilets in a factory with a double-flow area of a metropolis, respectively adding 500g of solid-state compound microbial agent (after activation) into three toilets filled with 5kg of filler, starting the device, and putting the toilets into use; while the other one is used as a reference without adding bactericide, samples are respectively taken after 7d, 14d, 21d and 30d to determine the volatile solid VS, odor intensity value and NH3Concentration and H2And (4) taking the average value of the S concentration. Evaluating the degradation performance of the microbial inoculum according to the change condition of VS, and according to odor degree and NH3Concentration and H2And evaluating the deodorization effect of the microbial inoculum according to the change condition of the S concentration. The measurement results are shown in Table 7 below.
TABLE 7 degradation and deodorization effects of microbial Agents
Figure BDA0002796570030000131
Example 4
Carrying out small batch test of 30 households, respectively filling 5kg of filler into a toilet bowl of each household, taking 15 households as a control group without adding a microbial inoculum, adding 500g of solid-state compound microbial inoculum (after activation) into the toilet bowl of each household, and starting a device for use; after the toilet runs for 7d, 14d, 21d and 30d respectively, samples were taken to determine volatile solids VS, odor intensity values, NH3Concentration and H2And (4) taking the average value of the S concentration. Evaluating the degradation performance of the microbial inoculum according to the change condition of VS, and according to odor degree and NH3Concentration and H2And evaluating the deodorization effect of the microbial inoculum according to the change condition of the S concentration. The measurement results are shown in Table 8 below. The results show that: the strain formed by the microbial inoculum is stable, the effect is improved, and the degradation and deodorization functions can still be exerted in farmers with higher daily use frequency.
TABLE 8 degradation and deodorization effects of the microbial inoculum
Figure BDA0002796570030000132
Figure BDA0002796570030000141
Example 5
Selecting eight mobile public toilets to install an ecological toilet in a NaLin lake scenic spot of an inner Mongolia autonomous region, adding 5kg of filler and 500g of solid compound microbial agent (after activation) into four of the mobile public toilets, and starting a device for use; compared with other four mobile public toilets without the bactericide. Samples were taken after 7d, 14d, 21d and 30d, respectively, to determine the volatile solids VS, odor intensity values, NH3Concentration and H2And (4) taking the average value of the S concentration. Evaluating the degradation performance of the microbial inoculum according to the change condition of VS, and according to odor degree and NH3Concentration and H2And evaluating the deodorization effect of the microbial inoculum according to the change condition of the S concentration. The measurement results are shown in Table 9 below. The results show that: even in high-cold arid areas, the complex microbial inoculum can still play the roles of degrading excrement and inhibiting odor generation.
TABLE 9 degradation and deodorization effects of the microbial Agents
Figure BDA0002796570030000142
The above-described embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solutions of the present invention can be made by those skilled in the art without departing from the spirit of the present invention, and the technical solutions of the present invention are within the scope of the present invention defined by the claims.

Claims (10)

1. The composite microbial inoculum for the environment-friendly ecological toilet is characterized in that: the complex microbial inoculum comprises pediococcus acidilactici and bacillus belgii.
2. The composite microbial inoculum for the environment-friendly ecological toilet as claimed in claim 1, which is characterized in that: the pediococcus acidilactici is preserved in the common microorganism center of China Committee for culture Collection of microorganisms, and the preservation number is CGMCC NO: 19770 and/or the Bacillus belmentioned is preserved in China general microbiological culture Collection center with the preservation number of CGMCC NO: 19769.
3. the composite microbial inoculum for the environment-friendly ecological toilet as claimed in claim 1, which is characterized in that: the bacterial liquid concentrations of the pediococcus acidilactici and the bacillus belgii are both 109CFU/mL。
4. The composite microbial inoculum for the environment-friendly ecological toilet as claimed in claim 3, which is characterized in that: in the composite microbial inoculum, the volume ratio of the pediococcus acidilactici to the bacillus belgii is 0.5-2: 0.5-2.
5. The composite microbial inoculum for the environmental ecological toilet as claimed in claim 1 or 2, which is characterized in that: the compound microbial inoculum also comprises microbial fruit ferment.
6. The composite microbial inoculum for the environment-friendly ecological toilet as claimed in claim 1, which is characterized in that: the microbial fruit ferment is prepared by inoculating lactobacillus and aroma-producing yeast into fruits and brown sugar, fermenting and filtering.
7. A preparation method of a composite microbial inoculum for an environment-friendly ecological toilet is characterized by comprising the following steps: activating Pediococcus acidilactici and Bacillus belgii, inoculating into liquid culture medium, and culturing until the bacterial liquid concentration is 109And CFU/mL, mixing the two bacterial liquids to obtain a liquid microbial inoculum, and finally adding microbial fruit enzyme according to the proportion of 10-15% to v/v in the liquid microbial inoculum to obtain the liquid composite microbial inoculum.
8. The preparation method of the composite microbial inoculum for the environmental ecological toilet as claimed in claim 7, which is characterized in that: adding an adsorbent into the liquid composite microbial inoculum, uniformly stirring, and drying to obtain a dried composite microbial inoculum; and then adding an accelerant into the dried complex microbial inoculum, and uniformly stirring to obtain the solid complex microbial inoculum.
9. The method for preparing the composite microbial inoculum for the environmental ecological toilet as claimed in claim 8, is characterized in that: the adsorbent is prepared by mixing bran, rice bran and vinasse, and the accelerant is yeast powder; in the liquid composite bacterial agent, the number of viable bacteria is more than or equal to 109CFU/mL, wherein the addition amount of the adsorbent is 0.5-2 kg/L; in the drying composite microbial inoculum, the addition amount of the accelerant is 4-7 g/kg.
10. The application of the composite microbial inoculum of any one of claims 1 to 6 in the deodorization treatment of the feces in an ecological toilet.
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