CN114534532A - Synthetic blood for detecting protective products and preparation method thereof - Google Patents
Synthetic blood for detecting protective products and preparation method thereof Download PDFInfo
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- CN114534532A CN114534532A CN202210094962.2A CN202210094962A CN114534532A CN 114534532 A CN114534532 A CN 114534532A CN 202210094962 A CN202210094962 A CN 202210094962A CN 114534532 A CN114534532 A CN 114534532A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The invention provides synthetic blood for detecting protective products and a preparation method thereof, wherein the synthetic blood comprises 20-25 parts of thickening agent, 0.5-0.8 part of surfactant, 8-12 parts of dye and 40-48 parts of electrolyte, wherein the thickening agent comprises the following preparation raw materials in parts by weight: polyurethane: polyacrylic acid: polyethylene glycol: ethylene glycol is 3-10:1-15:5-30: 2-5. The synthetic blood for detecting the protective product, which is prepared by the invention, has the advantages of rich raw material sources, strong operability, simple integral preparation process and short time consumption, is further favorable for improving the detection efficiency of the protective product and reducing the use of materials, and the surface tension of the synthetic blood for detecting the protective product, which is prepared by the method, meets the standard requirement and is favorable for improving the detection accuracy.
Description
Technical Field
The invention relates to the field of preparation of protective product performance test products, in particular to synthetic blood for detecting protective products and a preparation method thereof.
Background
Workers in the health care industry who treat and care for injured or ill patients are exposed to biological fluids that can spread disease. These diseases caused by various microorganisms can cause serious hazards to life and health. Medical personnel are required to wear surgical masks to avoid exposure to biological fluids with bacteria and viruses while working. To evaluate the ability of a medical surgical mask to protect the face of medical personnel from exposure to blood and body fluids that may transmit disease, a synthetic blood penetration test was conducted. The synthetic blood is an important test solution for the test, and the optimal preparation of the synthetic blood is one of the ways of ensuring the test accuracy, improving the detection efficiency and reducing the detection cost. However, in the process of preparing synthetic blood in the prior art, the problems of difficult purchase of raw materials, difficult dissolution of components such as thickening agents and the like, long time consumption, serious material waste and low detection efficiency exist.
In summary, the above problems still remain to be solved in the field of preparing synthetic blood for detection of protective products.
Disclosure of Invention
Based on the technical scheme, the invention provides the synthetic blood for detecting the protective product, and the specific technical scheme is as follows:
the synthetic blood for detecting the protective product comprises the following raw materials in parts by weight: 20-25 parts of thickening agent, 0.5-0.8 part of surfactant, 8-12 parts of dye and 40-48 parts of electrolyte;
the thickening agent comprises the following preparation raw materials in parts by weight: polyurethane: polyacrylic acid: polyethylene glycol: ethylene glycol 3-10:1-15:5-30: 2-5;
the preparation method of the thickening agent comprises the following steps: adding polyurethane and polyacrylic acid into a stirring kettle, and stirring at a stirring speed of 500-1500 r/min for 10-20 min to obtain a mixture A; uniformly mixing polyethylene glycol and ethylene glycol to obtain a mixture B; and then adding the mixture A into the mixture B, heating to 75-95 ℃, and continuing stirring for 1-3 h to obtain the thickening agent.
Further, the surfactant is tween 20.
Further, the dye is amaranth.
Further, the electrolyte is sodium chloride.
Further, the synthetic blood further comprises distilled water.
Further, the surface tension of the synthetic blood is 40mN/m to 44 mN/m.
The invention also provides a preparation method of the synthetic blood for detecting the protective product, which comprises the following steps:
adding distilled water into the thickening agent according to the material-liquid ratio of 1g to 400mL for pretreatment, stirring until the thickening agent is completely dissolved, then adding 5-8 times of distilled water, and continuing to perform magnetic stirring at the stirring speed of 1200r/min-1800r/min for later use;
and after cooling to room temperature, continuing adding the surfactant, the electrolyte and the dye, stirring at the stirring speed of 300r/min-800r/min for 10min-20min, adding 5-8 times of distilled water, stirring at the stirring speed of 50r/min-250r/min for 10min-30min, and finally adjusting the pH to 7.3 +/-0.1 by using a pH regulator to obtain the synthetic blood for detecting the protective product.
Further, the temperature of the distilled water added in the pretreatment is 75-90 ℃.
Further, the pH regulator is sodium hydroxide.
Further, the concentration of the sodium hydroxide is 2.5 mol/L.
The synthetic blood for detecting the protective product has the advantages of abundant raw material sources, strong operability, simple overall preparation process and short time, further contributes to improving the detection efficiency of the protective product, reduces the use of materials, meets the standard requirement on the surface tension of the synthetic blood for detecting the protective product, and contributes to improving the detection accuracy.
Drawings
FIG. 1 is a schematic drawing of a penetration test of synthetic blood prepared in example 1 of the present invention;
FIG. 2 is a schematic drawing of a penetration test of synthetic blood prepared in example 2 of the present invention;
FIG. 3 is a schematic drawing of a penetration test of synthetic blood prepared in example 3 of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail below with reference to embodiments thereof. It should be understood that the detailed description and specific examples, while indicating the scope of the invention, are intended for purposes of illustration only and are not intended to limit the scope of the invention.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.
The synthetic blood for detecting the protective product in one embodiment of the invention comprises the following raw materials in parts by weight: 20-25 parts of thickening agent, 0.5-0.8 part of surfactant, 8-12 parts of dye and 40-48 parts of electrolyte;
the thickening agent comprises the following preparation raw materials in parts by weight: polyurethane: polyacrylic acid: polyethylene glycol: ethylene glycol 3-10:1-15:5-30: 2-5;
the preparation method of the thickening agent comprises the following steps: adding polyurethane and polyacrylic acid into a stirring kettle, and stirring at a stirring speed of 500-1500 r/min for 10-20 min to obtain a mixture A; uniformly mixing polyethylene glycol and ethylene glycol to obtain a mixture B; and then adding the mixture A into the mixture B, heating to 75-95 ℃, and continuing stirring for 1-3 h to obtain the thickening agent.
In one embodiment, the surfactant is tween 20.
In one embodiment, the dye is amaranth.
In one embodiment, the electrolyte is sodium chloride.
In one embodiment, the synthetic blood further comprises distilled water.
In one embodiment, the synthetic blood has a surface tension of 40mN/m to 44 mN/m.
The invention also provides a preparation method of the synthetic blood for detecting the protective product, which comprises the following steps:
adding distilled water into the thickening agent according to the material-liquid ratio of 1g to 400mL for pretreatment, stirring until the thickening agent is completely dissolved, then adding 5-8 times of distilled water, and continuing to perform magnetic stirring at the stirring speed of 1200r/min-1800r/min for later use;
and after cooling to room temperature, continuing adding the surfactant, the electrolyte and the dye, stirring at the stirring speed of 300r/min-800r/min for 10min-20min, adding 5-8 times of distilled water, stirring at the stirring speed of 50r/min-250r/min for 10min-30min, and finally adjusting the pH to 7.3 +/-0.1 by using a pH regulator to obtain the synthetic blood for detecting the protective product.
In one embodiment, the temperature of the distilled water added in the pretreatment is 75 ℃ to 90 ℃.
In one embodiment, the pH adjusting agent is sodium hydroxide.
In one embodiment, the concentration of the sodium hydroxide is 2.5 mol/L.
The synthetic blood for detecting the protective product has the advantages of abundant raw material sources, strong operability, simple overall preparation process and short time, further contributes to improving the detection efficiency of the protective product, reduces the use of materials, meets the standard requirement on the surface tension of the synthetic blood for detecting the protective product, and contributes to improving the detection accuracy.
Embodiments of the present invention will be described in detail below with reference to specific examples.
Example 1:
a preparation method of synthetic blood for detecting protective products comprises the following steps:
adding 0.75g of polyurethane and 0.5g of polyacrylic acid into a stirring kettle, and stirring at a stirring speed of 1500r/min for 10min to obtain a mixture A; uniformly mixing 0.75g of polyethylene glycol and 0.5g of ethylene glycol to obtain a mixture B; then adding the mixture A into the mixture B, heating to 75 ℃, and continuing stirring for 3 hours to obtain a thickening agent;
adding 100mL of distilled water with the temperature of 85 ℃ into 2.5g of thickening agent for pretreatment, stirring until the thickening agent is completely dissolved, then adding 500mL of distilled water, and continuing to carry out magnetic stirring at the stirring speed of 1800r/min for later use;
and after cooling to room temperature, continuously adding 0.06g of Tween 20, 4.5g of sodium chloride and 1.0g of amaranth, stirring at the stirring speed of 800r/min for 10min, then stirring with 500mL of distilled water at the stirring speed of 50r/min for 10min, and finally adjusting the pH to 7.3 +/-0.1 by using sodium hydroxide to obtain the synthetic blood for detecting the protective product.
Example 2:
a preparation method of synthetic blood for detecting protective products comprises the following steps:
adding 0.75g of polyurethane and 0.5g of polyacrylic acid into a stirring kettle, and stirring at a stirring speed of 1500r/min for 10min to obtain a mixture A; uniformly mixing 0.75g of polyethylene glycol and 0.5g of ethylene glycol to obtain a mixture B; then adding the mixture A into the mixture B, heating to 85 ℃, and continuing stirring for 2h to obtain a thickening agent;
adding 100mL of distilled water with the temperature of 85 ℃ into 2.5g of thickening agent for pretreatment, stirring until the thickening agent is completely dissolved, then adding 500mL of distilled water, and continuing to carry out magnetic stirring at the stirring speed of 1200r/min for later use;
after cooling to room temperature, continuously adding 0.06g of Tween 20, 4.5g of sodium chloride and 1.0g of amaranth, stirring at a stirring speed of 300r/min for 20min, then stirring with 500mL of distilled water at a stirring speed of 50r/min for 10min, and finally adjusting the pH to 7.3 +/-0.1 by using sodium hydroxide to obtain the synthetic blood for detecting the protective product.
Example 3:
a preparation method of synthetic blood for detecting protective products comprises the following steps:
adding 0.6g of polyurethane and 0.4g of polyacrylic acid into a stirring kettle, and stirring at a stirring speed of 1000r/min for 20min to obtain a mixture A; uniformly mixing 0.6g of polyethylene glycol and 0.4g of ethylene glycol to obtain a mixture B; then adding the mixture A into the mixture B, heating to 80 ℃, and continuing stirring for 2h to obtain a thickening agent;
adding 100mL of distilled water with the temperature of 85 ℃ into 2.0g of thickening agent for pretreatment, stirring until the thickening agent is completely dissolved, then adding 500mL of distilled water, and continuing to carry out magnetic stirring at the stirring speed of 1400r/min for later use;
and after cooling to room temperature, continuously adding 0.06g of Tween 20, 4.5g of sodium chloride and 1.0g of amaranth, stirring for 15min at the stirring speed of 500r/min, stirring for 15min with 500mL of distilled water at the stirring speed of 200r/min, and finally adjusting the pH to 7.3 +/-0.1 by using sodium hydroxide to obtain the synthetic blood for detecting the protective product.
Comparative examples 1 to 3:
the only difference from example 1 is: the content of the thickener added was the same as in example 1 except that the content was changed, and the following table 1 shows details.
Table 1:
comparative example 4:
the only difference from example 1 is: the thickener was not pretreated and was otherwise the same as in example 1.
Comparative example 5:
the difference from example 1 is that: sodium carboxymethylcellulose was used as a thickener, and the other procedure was the same as in example 1.
Comparative example 6:
the only difference from example 2 is: the preparation method of the thickening agent comprises the following steps: the same procedure as in example 2 was repeated except that 0.75g of polyurethane, 0.75g of polyethylene glycol and 0.5g of ethylene glycol were mixed uniformly to prepare a thickener.
The dissolution properties of the thickeners of examples 1 to 3 and comparative examples 1 to 6 were observed, and the results are shown in table 2 below.
Table 2:
as can be seen from the data analysis of Table 1, the thickener prepared by the method has the remarkable advantage of high dissolution rate after being added into distilled water for pretreatment, and the dissolution effect of the thickener is also obviously superior to that of the conventional sodium carboxymethyl cellulose thickener. In addition, the components of the thickener have synergistic effect, and the thickener prepared by compounding the components with more excellent use effect meets the use requirement.
The surface tension tests of examples 1-3 and comparative examples 1-6 were carried out in accordance with GB/T22237-2008 "determination of surfactant surface tension", and the results are shown in Table 3 below.
Table 3:
as can be seen from the data analysis in Table 3, the surface tension of the synthetic blood prepared by the present invention meets the use requirements, the components and the mixture ratio of the components affect the surface tension of the synthetic blood, the penetration performance of the synthetic blood increases with the decrease of the surface tension, the surface tension of the human body fluid (excluding saliva) ranges from 42mN/m to 60mN/m, and the synthetic blood with the surface tension in the range of (42 +/-2) mN/m should be used to ensure the accuracy of the test.
The synthetic blood of examples 1 to 3 and the synthetic blood of comparative examples 1 to 6 were subjected to the penetration test, and 5 samples of the surgical mask were tested per group, and the results are shown in table 4 below.
Table 4:
as is clear from the analysis in Table 4, when the surface tension is more than 40mN/m, the inner surface of the medical surgical mask is free from the blood penetration phenomenon. When the surface tension is lower than 40mN/m, a minute amount of blood penetration phenomenon occurs on the inner surface of the mask. The synthetic blood for detecting the protective product has a more accurate detection result.
In addition, fig. 1 is a schematic diagram of a penetration test of the synthetic blood prepared in example 1, fig. 2 is a schematic diagram of a penetration test of the synthetic blood prepared in example 2, and fig. 3 is a schematic diagram of a penetration test of the synthetic blood prepared in example 3, it can be seen from fig. 1 to 3 that the synthetic blood prepared by the present invention can conform to the detection field of the medical surgical mask, and the synthetic blood is spread out to a similar extent on the outer surface of the medical surgical mask, which illustrates that the synthetic blood prepared by the present invention has a more stable advantage.
All possible combinations of the technical features of the above embodiments may not be described for the sake of brevity, but should be considered as within the scope of the present disclosure as long as there is no contradiction between the combinations of the technical features.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (10)
1. The synthetic blood for detecting the protective product is characterized by comprising the following raw materials in parts by weight: 20-25 parts of thickening agent, 0.5-0.8 part of surfactant, 8-12 parts of dye and 40-48 parts of electrolyte;
the thickening agent comprises the following preparation raw materials in parts by weight: polyurethane: polyacrylic acid: polyethylene glycol: ethylene glycol 3-10:1-15:5-30: 2-5;
the preparation method of the thickening agent comprises the following steps: adding polyurethane and polyacrylic acid into a stirring kettle, and stirring at a stirring speed of 500-1500 r/min for 10-20 min to obtain a mixture A; uniformly mixing polyethylene glycol and ethylene glycol to obtain a mixture B; and then adding the mixture A into the mixture B, heating to 75-95 ℃, and continuing stirring for 1-3 h to obtain the thickening agent.
2. The synthetic blood for assay of a protective product according to claim 1 wherein said surfactant is tween 20.
3. The synthetic blood for detection of protective products of claim 1 wherein said dye is amaranth.
4. The synthetic blood for assay of a protective product according to claim 1, wherein said electrolyte is sodium chloride.
5. The synthetic blood for assay of a protective product according to claim 1, wherein the synthetic blood further comprises distilled water.
6. The synthetic blood for assay of a protective product according to claim 1, wherein the synthetic blood has a surface tension of 40mN/m to 44 mN/m.
7. A method for preparing synthetic blood for testing protective products according to any one of claims 1 to 6, comprising the following steps:
adding distilled water into the thickening agent according to the material-liquid ratio of 1g to 400mL for pretreatment, stirring until the thickening agent is completely dissolved, then adding 5-8 times of distilled water, and continuously performing magnetic stirring at the stirring speed of 1200r/min-1800r/min for later use;
and after cooling to room temperature, continuing adding the surfactant, the electrolyte and the dye, stirring at the stirring speed of 300r/min-800r/min for 10min-20min, adding 5-8 times of distilled water, stirring at the stirring speed of 50r/min-250r/min for 10min-30min, and finally adjusting the pH to 7.3 +/-0.1 by using a pH regulator to obtain the synthetic blood for detecting the protective product.
8. The method for preparing synthetic blood for assay of a protective product according to claim 7, wherein the temperature of the distilled water added in the pretreatment is 75 ℃ to 90 ℃.
9. The method of claim 8, wherein the pH adjusting agent is sodium hydroxide.
10. The method for preparing synthetic blood for assay of a protected product according to claim 8, wherein the concentration of sodium hydroxide is 2.5 mol/L.
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