CN114533142B - Biopsy puncture gun for rapidly detecting benign and malignant tissue - Google Patents
Biopsy puncture gun for rapidly detecting benign and malignant tissue Download PDFInfo
- Publication number
- CN114533142B CN114533142B CN202210164762.XA CN202210164762A CN114533142B CN 114533142 B CN114533142 B CN 114533142B CN 202210164762 A CN202210164762 A CN 202210164762A CN 114533142 B CN114533142 B CN 114533142B
- Authority
- CN
- China
- Prior art keywords
- needle
- box body
- puncture
- cavity
- gun
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
- A61B10/02—Instruments for taking cell samples or for biopsy
- A61B10/0233—Pointed or sharp biopsy instruments
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The application relates to the field of medical equipment, in particular to a biopsy puncture gun for quickly detecting benign and malignant tissue, which comprises a handle part, a box body and a sleeve which are connected in sequence; needle cavities are arranged in the handle part, the box body and the sleeve, the needle cavities linearly extend from one end of the handle part, which is far away from the box body, to one end of the sleeve, which is far away from the box body, through the box body, and a puncture needle matched with the needle cavities is also arranged in the needle cavities; a reaction groove passing cavity is formed in one side wall of the box body, and the box body further comprises a reaction groove which is communicated with the needle cavity through the cavity; still including being located the pressure cutting part of reaction tank top, the pjncture needle be equipped with press cutting part matched with through-hole, still be equipped with the syringe connecting piece on the box body, the syringe connecting piece includes the injection mouth, injection mouth and reaction tank intercommunication. The biopsy puncture gun is characterized by comprising a puncture collection device, a sheet making device, a specificity marking device, a mechanical transmission device and a biopsy device.
Description
Technical Field
The application relates to the field of medical equipment, in particular to a biopsy puncture gun for rapidly detecting benign and malignant tissue.
Background
Preoperative biopsy is the most important part of diagnosis pathology, and is clinically used for carrying out preoperative biopsy puncture on tissues suspected to be malignant, and carrying out pathological examination can definitely determine benign and malignant tissues and guide the establishment of important diagnosis and treatment decisions such as surgical operation, chemotherapy scheme and the like. At present, biopsy puncture often depends on puncture under image guidance such as ultrasound and CT or a traditional blind puncture technology, and tissue most likely to be malignant is difficult to ensure to obtain through image observation or freehand operation, and tissue with pathological diagnosis significance is often difficult to clearly display, so that tissue most likely to be malignant is difficult to ensure to obtain through image observation or freehand operation, thereby leading to missed diagnosis of malignant lesion results in subsequent pathological diagnosis steps, or leading to repeated puncture of a patient, and increasing injury to the patient. A more reliable instrument for identifying benign and malignant diseases during a puncturing operation is desired to be developed.
Disclosure of Invention
In view of the above-mentioned drawbacks of the prior art, an object of the present application is to provide a biopsy puncture gun for rapid detection of benign and malignant tissue, which can rapidly give a preliminary puncture result to a clinician at the bedside of a biopsy operation, prompt benign and malignant tissue, assist the clinician in making a decision, and reduce unnecessary injuries to a patient. The invention mainly depends on the carbon-nitrogen quantum dot with the tumor specific marking function, the puncture gun is simple and integrated, the trouble of doctor operation is reduced as much as possible, the practicability is increased, and the invention is used for solving the problems in the existing operation.
To achieve the above and other related objects, the present application provides a biopsy puncture gun for rapid detection of benign and malignant tissue, comprising a handle portion, a box body and a sleeve connected in sequence; the needle cavity is arranged in the handle part, the box body and the sleeve, the end, away from the box body, of the needle cavity from the handle part extends to the end, away from the box body, of the sleeve through the box body in a straight line, and a puncture needle matched with the needle cavity is further arranged in the needle cavity; a reaction groove passing cavity is formed in one side wall of the box body, and the box body further comprises a reaction groove matched with the reaction groove passing cavity, wherein the reaction groove passing cavity is communicated with the needle cavity; still including being located the pressure cutting part of reaction tank top, the pjncture needle be equipped with press cutting part matched with through-hole, still be equipped with the syringe connecting piece on the box body, the syringe connecting piece includes the injection mouth, injection mouth and reaction tank intercommunication.
In some embodiments of the invention, the device further comprises a puncture needle base, wherein the puncture needle base is connected with the puncture needle; one end of the handle part, which is far away from the box body, is provided with a base accommodating cavity; the base receiving cavity communicates with the needle cavity.
In some embodiments of the present invention, the handle portion is provided with a sliding rail, the puncture needle base is provided with a sliding block capable of sliding along the sliding rail, and the extending direction of the sliding rail is parallel to the extending direction of the puncture needle.
In some embodiments of the present invention, the puncture needle base is provided with a marble, the handle part is provided with a first clamping groove matched with the marble, the handle part is provided with a pressing block, and the extending direction of the pressing block is perpendicular to the extending direction of the puncture needle; the pressing block can penetrate through the first clamping groove to press the marble; the handle part is provided with a first elastic piece, and the first elastic piece is in axial abutting connection with the puncture needle base; the pressing block is sleeved with a second elastic piece, and the second elastic piece is in circumferential abutting connection with the puncture needle base.
In some embodiments of the invention, the handle portion is removably attached to the case; the box body is detachably connected with the sleeve.
In some embodiments of the present invention, a rotary plug is provided at an end of the handle portion away from the base of the puncture needle, and a plug slot is provided on the case body, the plug slot being in communication with the needle cavity.
In some embodiments of the invention, a waste liquid tank is also provided in the cartridge.
In some embodiments of the invention, the height of the injection port is greater than the height of the reaction slot.
In some embodiments of the invention, the reaction tank further comprises a grip portion, the grip portion being located outside the cartridge body.
In some embodiments of the invention, the other side wall of the box body is provided with a cover plate passing cavity, and the cover plate is communicated with the needle cavity through the cavity; the reaction tank is also provided with a cover plate which can pass through the cover plate and is covered on the reaction tank through the cavity.
In some embodiments of the present invention, the pressing and cutting part includes a pressing button, a pressing sheet, and a third elastic member for resetting, which are connected; one end of the pressing piece, which is far away from the pressing piece button, is provided with a cutting edge.
In some embodiments of the invention, the top of the case is made of transparent material.
In some embodiments of the invention, the puncture needle comprises a needle point, and a needle groove is arranged at one end of the puncture needle, which is close to the needle point; the number of the through holes is multiple, one part of the through holes are arranged on the needle point, and the other part of the through holes are arranged on the needle groove.
In some embodiments of the invention, further comprising a syringe comprising carbon nitrogen fluorescent quantum dots therein.
Compared with the prior art, the beneficial effects of this application are:
the biopsy puncture gun for rapidly detecting benign and malignant tissue is a biopsy puncture gun with integrated puncture collection, sheet production and specific marking, can conveniently take out a special slide for identifying benign and malignant tissue while ensuring the normal operation of the existing biopsy puncture procedure through the design of an exquisite mechanical transmission device, conveniently completes real-time and sensitive tissue benign and malignant identification based on carbon-nitrogen quantum dots with tumor specific marking functions, and can still completely send the residual tissue to pathological examination. The integrated gun type design is adopted, so that the operation is convenient to hold, the operation trouble can be reduced, the pollution or loss generated during sample transfer can be avoided, the practicability is high, the use convenience and experience of clinicians are improved, and the device is more convenient and practical than a clinically common puncture needle.
Drawings
FIG. 1 is a schematic perspective view of a biopsy puncture gun for rapid detection of benign and malignant tissue in the present application.
FIG. 2 is a schematic perspective view of a biopsy puncture gun for rapid detection of benign and malignant tissue in the present application.
FIG. 3 is a schematic diagram of the split structure of the biopsy puncture gun for rapid detection of benign and malignant tissue in the present application.
FIG. 4 is a schematic view of the partial structure of the handle portion and the cartridge portion of the tissue benign and malignant rapid detection biopsy puncture gun of the present application.
Fig. 5 is a schematic partial structure of a cartridge body of the biopsy puncture gun for rapid detection of benign and malignant tissue in the present application.
FIG. 6 is a schematic diagram of the reaction well and cover plate structure of the biopsy puncture gun for rapid detection of benign and malignant tissue in the present application.
Fig. 7 is a schematic view showing the partial structure of a puncture needle of a biopsy puncture gun for rapid detection of benign and malignant tissue in the present application.
FIG. 8 is a schematic structural view of the rapid detection biopsy puncture gun for benign and malignant tissue of the present application.
Description of element reference numerals
1. Handle portion
11. Base accommodating cavity
12. Sliding rail
13. First clamping groove
14. Press block
15. First elastic piece
16. Rotary plug-in block
17. Pulling-out button
2. Box body
21. Reaction tank passing cavity
22. Reaction tank
221. Holding part
222. Second clamping groove
23. Pressing and cutting part
231. Tabletting press button
232. Tabletting
233. Third elastic piece
24. Syringe connector
241. Injection port
25. Insertion groove
26. Waste liquid tank
27. Cover plate passing cavity
28. Cover plate
29. Threaded hole
3. Casing pipe
31. Sleeve connecting block
4. Needle cavity
5. Puncture needle
51. Through hole
52. Needle tip
53. Needle groove
6. Puncture needle base
61. Sliding block
62. Marble ball
63. Needle body connecting block
7. Fixing ring
Detailed Description
In the description of the present application, it should be noted that, the devices, proportions, sizes, etc. shown in the drawings attached to the present application are merely used in conjunction with the disclosure of the present application, and are not intended to limit the applicable limitations of the present application, so that the modification, the change of the proportions, or the adjustment of the sizes of any devices should not be construed as technically essential, and without affecting the efficacy and the achievement of the purposes of the present application, the modifications shall still fall within the scope of the technical content disclosed in the present application. Also, references to orientations or positional relationships of the terms "center", "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., are based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present application and simplifying the description, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be configured and operated in a particular orientation, and therefore should not be construed as limiting the present application. Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present application, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the terms in this application will be understood by those of ordinary skill in the art as the case may be.
Furthermore, in the description of the present application, unless otherwise indicated, the meaning of "a plurality" is two or more.
As shown in fig. 1 to 8, an embodiment of the present application provides a biopsy puncture gun for rapid detection of benign and malignant tissue, comprising a handle portion 1, a box body 2 and a sleeve 3 connected in sequence; the handle part 1, the box body 2 and the sleeve 3 are internally provided with a needle cavity 4, the needle cavity 4 extends from one end of the handle part 1 far away from the box body 2 to one end of the sleeve 3 far away from the box body 2 through the box body 2 in a straight line, the needle cavity 4 is also internally provided with a puncture needle 5 matched with the needle cavity 4, the extending direction of the puncture needle 5 is consistent with the extending direction of the needle cavity 4, and the puncture needle 5 can reciprocate relative to the needle cavity 4; a reaction tank passing cavity 21 is arranged on one side wall of the box body 2, and the box body further comprises a reaction tank 22 matched with the reaction tank passing cavity 21, wherein the reaction tank 22 is inserted into the box body 2 after passing through the reaction tank passing cavity 21. The reaction tank is communicated with the needle cavity 4 through a cavity 21; and a pressing and cutting part 23 above the reaction tank 22, wherein the pressing and cutting part 23 is arranged on the box body 2 in normal condition. The puncture needle 5 is provided with a through hole 51 which is matched with the pressing and cutting part 23, and the through hole 51 can be positioned at one end of the puncture needle 5 which is intended to contact with tissues in general. The box body 2 is also provided with a syringe connecting piece 24, the syringe connecting piece 24 comprises an injection port 241, and the injection port 241 is communicated with the reaction tank 22. When the biopsy puncture gun is applied, the puncture needle 5 moves towards the direction close to the pre-puncture tissue relative to the needle cavity 4, after the tissue to be detected is taken down, moves towards the direction close to the handle part 1 relative to the needle cavity 4, when the puncture needle 5 moves to the box body 2, when the through hole 51 on the puncture needle 5 is in a straight line with the pressing and cutting part 23, at least part of the tissue to be detected in the through hole 51 can be cut down and falls into the reaction tank 22 by pressing the pressing and cutting part 23 towards the needle cavity 4, the detection liquid containing carbon-nitrogen fluorescent quantum dots is injected through the injection port 241, and the normal cells almost have no fluorescent emission after being detected by the fluorescence spectrophotometer or observed under the naked eye of the fluorescence microscope, so that whether tumor cells exist in the tissue or not can be identified.
In the biopsy puncture gun for rapidly detecting benign and malignant tissue provided by the invention, as shown in fig. 3, the biopsy puncture gun further comprises a puncture needle base 6, wherein the puncture needle base 6 is connected with a puncture needle 5; as shown in fig. 1, a base accommodating cavity 11 is arranged at one end of the handle part 1 away from the box body 2; the base receiving chamber 11 communicates with the needle chamber 4 and the lancet base 6 is reciprocally movable relative to the base receiving chamber 11.
In some embodiments, the puncture needle base 6 is detachably connected with the puncture needle 5, so that replacement and storage are convenient.
In some embodiments, the size of the lancet base 6 is larger than the diameter of the lancet 5, thereby further limiting the range of motion of the lancet 5 within the needle cavity 4.
In other embodiments, as shown in FIG. 3, the lancet base 6 includes a needle hub 63, with the needle hub 63 being removably connected to the lancet 5. The needle body connecting block 63 is provided with a needle body accommodating cavity, the puncture needle 5 can be embedded into the needle body accommodating cavity, and the puncture needle 5 is spliced with the needle body connecting block 63. The size of the lancet base 6 is larger than the diameter of the needle connecting block 63, and the diameter of the needle connecting block 63 is larger than the diameter of the lancet 5.
In the biopsy puncture gun for rapidly detecting benign and malignant tissue provided by the invention, as shown in fig. 4, a sliding rail 12 is arranged on the handle part 1, a sliding block 61 capable of sliding along the sliding rail 12 is arranged on the puncture needle base 6, and the extending direction of the sliding rail 12 is parallel to the extending direction of the puncture needle 5. By the cooperation of the slider 61 on the puncture needle base 6 and the slide rail 12 of the handle portion 1, the movement direction of the puncture needle 5 can be stabilized.
In the biopsy puncture gun for rapidly detecting benign and malignant tissue provided by the invention, as shown in fig. 1 and 4, a marble 62 is arranged on the puncture needle base 6, and a first clamping groove 13 matched with the marble 62 is arranged on the handle part 1. When the marble 62 is engaged with the first engaging groove 13, the positional relationship between the puncture needle 5 and the needle cavity 4 is maintained, and the tissue can be punctured. In some embodiments, a marble 62 is provided on the needle connection block 63.
In the biopsy puncture gun for rapidly detecting benign and malignant tissue provided by the invention, as shown in fig. 1, a pressing block 14 is arranged on a handle part 1, and the extending direction of the pressing block 14 is perpendicular to the extending direction of a puncture needle 5; the pressing block 14 can pass through the first clamping groove 13 to press the marble 62; the handle part 1 is provided with a first elastic piece 15, and the first elastic piece 15 is in axial abutting connection with the puncture needle base 6; however, when the pressing block 14 moves toward the ball 62, the ball 62 is pressed, so that the ball 62 is separated from the first clamping groove 13, and the puncture needle base 6 and the puncture needle 5 are rapidly pulled away under the action of the first elastic member 15, and the tissue in the body is carried out. The pressing block 14 is sleeved with a second elastic member (not shown), and the second elastic member is in circumferential abutting connection with the puncture needle base 6. After the tissue is taken out, the puncture needle base 6 is pushed to move towards the direction of the box body 2, the first elastic piece is reset, and the marble 62 returns to the first clamping groove 13 to be clamped. At this time, the second elastic member is reset by moving the pressing block 14 in a direction away from the marble 62. In some embodiments, the first elastic member 15 or the second elastic member may be, for example, a spring. In some embodiments, the end of the push block 14 remote from the marble 62 is provided with a pull-off button 17.
In the biopsy puncture gun for rapidly detecting benign and malignant tissue, the handle part 1 is detachably connected with the box body 2; the box body 2 and the sleeve 3 are detachably connected, so that the box is convenient to install and store.
In the biopsy puncture gun for rapidly detecting benign and malignant tissue provided by the invention, as shown in fig. 3, a rotary plug-in block 16 is arranged at one end of the handle part 1 far away from the puncture needle base 6, as shown in fig. 4, a plug-in groove 25 matched with the rotary plug-in block 16 is arranged on the box body 2, and the plug-in groove 25 is communicated with the needle cavity 4. More specifically, in some embodiments, the outer periphery of the rotary plug-in block 16 is provided with a plurality of protrusions, the outer periphery of the plug-in groove 25 is provided with grooves corresponding to the protrusions one by one, and after the rotary plug-in block 16 is inserted into the plug-in groove 25, the handle base is rotated, so that the protrusions and the grooves are fixedly connected one by one.
In the biopsy puncture gun for rapidly detecting benign and malignant tissue provided by the invention, as shown in fig. 1, a waste liquid tank 26 is also arranged in the box body 2. After the solution injected through the injection port 241 enters the reaction tank 22, one side edge of the reaction tank 22 is leveled with the inner edge of the reaction box, so that the excessive waste liquid can flow into the waste liquid tank 26, the injected liquid amount can be prevented from being excessive, and the cleaning is convenient. In some preferred embodiments, the bottom of waste tank 26 is provided with a waste drain that can be opened and closed.
In the biopsy puncture gun for rapidly detecting benign and malignant tissue provided by the invention, the height of the injection port 241 is higher than that of the notch of the reaction tank 22, so that injection liquid can conveniently enter the reaction tank 22.
In the rapid tissue benign and malignant detection biopsy puncture gun provided by the invention, as shown in fig. 2, the reaction tank 22 further comprises a holding part 221, and the holding part 221 is positioned outside the box body 2. The holding portion 221 on the reaction tank 22 facilitates the drawing of the reaction tank 22 from the case 2.
In the rapid detection biopsy puncture gun for benign and malignant tissue provided by the invention, the capacity of the reaction tank 22 is a fixed value, so that the accurate volume ratio of the reaction solution to the tissue cells is ensured. In some embodiments, the reaction tank 22 has a side length of 5 to 15mm and a depth of 0.5 to 1.5mm.
In the biopsy puncture gun for rapidly detecting benign and malignant tissue provided by the invention, as shown in fig. 5, a cover plate passing cavity 27 is arranged on the other side wall of the box body 2, and the cover plate is communicated with the needle cavity 4 through the cavity 27; as in fig. 1, a lid 28 is also included which can be passed through the lid through chamber 27 to be closed to the reaction tank 22. The cartridge 2, the reaction vessel 22, and the cover 28 cooperate. In some embodiments, the reaction tank 22 is provided with a second slot 222 that mates with the cover 28. More specifically, the second clamping grooves 222 may be provided on both sides of the reaction groove 22 or may be provided in the holding portion 221. Thus, the cover plate 28 can be stabilized on the reaction tank 22 to form an integrated structure, and is convenient to take out for subsequent detection. The reaction tank 22 is specifically structured to be inserted into the reaction cassette and to have the cover sheet 28 stably fitted thereon.
In the biopsy punch gun for rapid detection of benign and malignant tissue according to the present invention, as shown in fig. 5, the pressing and cutting portion 23 includes a pressing button 231, a pressing piece 232, and a third elastic member 233 for resetting. The end of the pressing piece 232 away from the pressing piece button 231 is provided with a cutting edge. In some embodiments, the housing 2 is provided with a housing cavity for housing the third elastic member 233, and the housing cavity includes two third elastic members 233, which are symmetrically disposed in the housing 2, respectively, and the pressing piece 232 is located between the two third elastic members 233, and the extending direction of the pressing piece 232 is parallel to the extending direction of the third elastic members 233. In some embodiments, the third elastic member 233 may be a spring, for example.
In the biopsy puncture gun for rapidly detecting benign and malignant tissue provided by the invention, as shown in fig. 5, a fixing ring 7 for fixing the puncture needle 5 is further arranged in the box body 2, and the diameter of the fixing ring 7 is slightly larger than that of the puncture needle 5, so that the puncture needle 5 can pass through the fixing ring 7 but is not too swayed.
In the rapid tissue benign and malignant detection biopsy puncture gun provided by the invention, the top of the box body 2 is made of transparent material, for example, glass. The relative positions of the through hole 51 on the puncture needle 5 and the pressing and cutting portion 23 are easily observed.
In the biopsy puncture gun for rapidly detecting benign and malignant tissue provided by the invention, as shown in fig. 7, the puncture needle 5 comprises a needle point 52, and a needle groove 53 is arranged at one end, close to the needle point 52, of the puncture needle 5; the number of the through holes 51 is plural, one part of the through holes 51 is arranged on the needle tip 52, and the other part of the through holes 51 is arranged on the needle groove 53. The needle groove 53 is a semi-packed structure that reduces the pressure within the needle and facilitates the removal of tissue cells from the body. The through-holes 51 in the needle tip 52 and the needle groove 53 cooperate with the pressing and cutting portion 23 to cut a part of the tissue into the reaction groove 22.
In the biopsy puncture gun for quickly detecting benign and malignant tissue, the inner diameter of the sleeve 3 is slightly larger than the diameter of the puncture needle 5, so that the puncture needle 5 can move in the sleeve 3 but cannot shake too much. In some embodiments, the sleeve 3 is provided with a sleeve connection block 31, and the sleeve 3 is connected with the box body 2 through the sleeve connection block 31, for example, the sleeve connection block can be in threaded connection, and in fig. 3, the box body 2 is provided with a threaded hole 29 matched with the sleeve connection block 31. The puncture needle 5 can pass through the tissue cell and is matched with puncture to take out the tissue cell.
The rapid tissue benign and malignant detection biopsy puncture gun provided by the invention further comprises an injector, wherein the injector comprises carbon-nitrogen fluorescence quantum dots. The carbon-nitrogen fluorescent quantum dots used in the invention have a specific labeling function on tumor cells. The carbon-nitrogen fluorescent quantum dot is 3.2nm in average diameter prepared by a 2, 3-diaminophenazine hydrothermal method, has good quantum dot yield and photoluminescence characteristics, can enter cells through cell membranes, and emits fluorescence of about 530nm under excitation of fluorescence of about 400 nm. NAD+ in living tissue can obviously increase fluorescence value excited by carbon nitrogen fluorescence quantum dots, and NAD+ level in cytoplasm of normal cells is only 1-10 multiplied by 10 -6 M, whereas nad+ levels in the cytoplasm of tumor cells are significantly increased due to abnormal glycolysis. After the incubation reaction of the carbon-nitrogen fluorescent quantum dot solution and the excitation of the fluorescent light irradiation at 400nm, tumor cells hidden in the tissue emit stronger fluorescence, and normal cells almost have no fluorescence emission, so that whether the tumor cells exist in the tissue can be identified. After the puncture is finished, a benign and malignant result can be obtained through fluorescence detection only for 5-10min, and the next puncture or inspection decision is guided. The traditional bedside puncture requires a clinician to select different angles for multiple puncture tests according to influence observation or personal experience, and then the puncture tested tissues are sent to a pathology department for examination, and the possibility of missing key tissues still exists.The invention directly collects and detects at the bedside in one step to obtain a primary benign and malignant identification result, assists a clinician in making a decision whether to re-puncture or not, provides an important reference for the subsequent diagnosis of a pathologist, and reduces unnecessary injuries to patients. The initial detection consumes little tissue and hardly affects the subsequent pathological examination.
The application process of the tissue benign and malignant rapid detection biopsy puncture gun (as shown in figures 1-8) comprises the following steps:
1) The handle part 1, the box body 2 and the sleeve 3 are connected, the puncture needle 5 and the puncture needle base 6 are connected through the needle body connecting block 63, the needle cavity 4 linearly extends from one end of the handle part 1 far away from the box body 2 to one end of the sleeve 3 far away from the box body 2 through the box body 2, and the puncture needle 5 penetrates through the needle cavity 4.
2) The puncture needle 5 is inserted into the tumor to be examined of the patient by bare hands or under the guidance of an image such as ultrasound.
3) When the push button 17 on the handle part 1 is pressed down and the pressing block 14 moves towards the marble 62, the marble 62 is extruded, so that the marble 62 is separated from the first clamping groove 13, under the action of the first elastic piece 15, the puncture needle base 6 and the puncture needle 5 are rapidly pulled away, the tissue in the body is brought out, and then the puncture needle base 6 is pulled in the direction away from the tissue in the body manually, so that the through hole 51 (serving as a first sampling point) on the needle tip 52 of the puncture needle 5 is positioned right below the pressing piece 232.
4) Pressing the press button 231 causes a small portion (about 0.4mm by 6 um) of the tissue to be examined in the needle 5 to be cut into the square recess in the reaction well 22 (square recess slide) to form a thin to light transmissive slice.
5) The carbon-nitrogen fluorescence quantum dot solution is injected into the gun body from the injection port 241 at the side of the gun body by using a micro injector, the liquid flows into the reaction tank 22 along the guide pipe, fully contacts with the tissue slice, and the redundant liquid flows into the waste liquid tanks 26 at the two sides.
6) Standing for 2-3min to allow the mixture to incubate fully, slowly injecting about 1ml of phosphate buffer PBS from a liquid inlet by using a micro-injector, and washing the slices fully.
7) A cover slip 28 (cover slip) is inserted from the side cover slip of the box body 2 through the cavity 27 to form a complete cover slip, and the reaction tank 22 covered with the cover slip 28 is completely taken out from the reaction tank through the cavity 21 at the corresponding other side of the box body 2.
8) And (3) placing the cover glass into a spectrophotometer to measure the fluorescence value of the specimen, and comparing the fluorescence value range of tumor cells to judge whether tumor cells exist in the obtained tissue.
9) After the above steps are completed, a new reaction groove 22 is inserted from the side of the case 2, the puncture needle 5 is moved, and the through hole 51 (second sampling point) of the needle groove 53 in the middle of the puncture needle 5 is moved to the position right under the pressing piece 232. Repeating the above steps 4) -8).
10 Taking out the puncture needle base 6, the puncture needle 5 and the sleeve 3 from the tail end of the puncture gun, and filling the residual tissues into a sterile sample-feeding bag for further detection in a pathology department. If the two fluorescence value detection indicates no tumor cells, the puncture needle 5 can be replaced according to clinical requirements to perform secondary puncture.
According to the biopsy puncture gun for rapidly detecting benign and malignant tissue, provided by the invention, a principle that carbon and nitrogen quantum dots are specifically combined with tumor cells to carry out imaging marking is utilized, a spectrophotometer is utilized to rapidly display a puncture result on a biopsy puncture site, whether tumor cells exist in a puncture acquisition tissue is judged, and a clinician is assisted to carry out next puncture or pathological examination decision. For patients with high suspicion of malignant lesions, if the result shows that the obtained tissue is free of tumor cells, a clinician can select a replacement part to puncture again, so that the possibility of missed diagnosis is reduced; if tumor cells are present, the clinician may combine with the patient's clinical manifestation to reduce unnecessary re-punctures. In addition, the result of the tumor cell specific marker can be used for a pathologist to refer to, and the tumor cell property can be accurately judged by combining the results of subsequent microscopic observation, karyotype analysis, immunohistochemistry and the like.
The carbon-nitrogen fluorescent quantum dot used in the invention is a high-sensitivity tumor cell probe, NAD+ can obviously increase the fluorescence intensity excited by the carbon-nitrogen fluorescent quantum dot, and the NAD+ level in tumor cells is obviously higher than that of normal cells. Compared with the existing in-vitro tumor detection solution, the carbon-nitrogen fluorescence quantum dot has more sensitive and specific tumor marking performance, and has innovation and breakthrough.
In conclusion, the biopsy puncture gun for rapidly detecting benign and malignant tissue provided by the embodiment of the invention is designed for puncture collection, sheet making and specific marking, and through the design of an exquisite mechanical transmission device, a special slide can be conveniently taken out for identifying benign and malignant tissue while the normal operation of the existing biopsy puncture procedure is ensured, so that real-time and sensitive tissue benign and malignant identification is conveniently completed, and the residual tissue can still be completely sent to pathological examination. The integrated gun type design is adopted, so that the operation is convenient to hold, the operation trouble can be reduced, the pollution or loss generated during sample transfer can be avoided, the practicability is high, the use convenience and experience of clinicians are improved, and the device is more convenient and practical than the clinical common puncture needle 5.
In conclusion, the method and the device effectively overcome various defects in the prior art and have high industrial utilization value.
The foregoing embodiments are merely illustrative of the principles of the present application and their effectiveness, and are not intended to limit the application. Modifications and variations may be made to the above-described embodiments by those of ordinary skill in the art without departing from the spirit and scope of the present application. Accordingly, it is intended that all equivalent modifications and variations which may be accomplished by persons skilled in the art without departing from the spirit and technical spirit of the disclosure be covered by the claims of this application.
Claims (10)
1. The biopsy puncture gun for rapidly detecting benign and malignant tissue is characterized by comprising a handle part (1), a box body (2) and a sleeve (3) which are connected in sequence; the novel medical needle is characterized in that needle cavities (4) are arranged in the handle part (1), the box body (2) and the sleeve (3), the needle cavities (4) linearly extend from one end, far away from the box body (2), of the handle part (1) to one end, far away from the box body (2), of the sleeve (3), and a puncture needle (5) matched with the needle cavities is further arranged in the needle cavities (4); a reaction groove passing cavity (21) is formed in one side wall of the box body (2), and the box body further comprises a reaction groove (22) matched with the reaction groove passing cavity (21), wherein the reaction groove is communicated with the needle cavity (4) through the cavity (21); the device further comprises a pressing and cutting part (23) positioned above the reaction tank (22), the puncture needle (5) is provided with a through hole (51) matched with the pressing and cutting part (23), the box body (2) is further provided with a syringe connecting piece (24), the syringe connecting piece (24) comprises an injection port (241), and the injection port (241) is communicated with the reaction tank (22); a cover plate passing cavity (27) is formed in the other side wall of the box body (2), and the cover plate is communicated with the needle cavity (4) through the cavity (27); the reaction tank also comprises a cover plate (28) which can pass through the cover plate and is covered on the reaction tank (22) through the cavity (27); the pressing and cutting part (23) comprises a pressing button (231), a pressing piece (232) and a third elastic piece (233) for resetting which are connected; one end of the pressing piece (232) far away from the pressing piece button (231) is provided with a cutting edge; the puncture needle (5) comprises a needle point (52), and a needle groove (53) is formed in one end, close to the needle point (52), of the puncture needle (5); the number of the through holes (51) is multiple, one part of the through holes (51) are arranged on the needle point (52), and the other part of the through holes (51) are arranged on the needle groove (53); the device also comprises an injector, wherein the injector comprises carbon-nitrogen fluorescent quantum dots.
2. The rapid tissue wellness detection biopsy puncture gun of claim 1, further comprising a puncture needle base (6), said puncture needle base (6) being connected to a puncture needle (5); one end of the handle part (1) far away from the box body (2) is provided with a base accommodating cavity (11); the base accommodating cavity (11) is communicated with the needle cavity (4).
3. The biopsy puncture gun for rapid detection of benign and malignant tissue according to claim 2, wherein a slide rail (12) is provided on the handle portion (1), a slider (61) capable of sliding along the slide rail (12) is provided on the puncture needle base (6), and an extending direction of the slide rail (12) is parallel to an extending direction of the puncture needle (5).
4. The biopsy puncture gun for rapid detection of benign and malignant tissue according to claim 2, characterized in that a marble (62) is arranged on the puncture needle base (6), a first clamping groove (13) matched with the marble (62) is arranged on the handle part (1), a pressing block (14) is arranged on the handle part (1), and the extending direction of the pressing block (14) is perpendicular to the extending direction of the puncture needle (5); the pressing block (14) can penetrate through the first clamping groove (13) to press the marble (62); a first elastic piece (15) is arranged on the handle part (1), and the first elastic piece (15) is in axial abutting connection with the puncture needle base (6); the pressing block (14) is sleeved with a second elastic piece, and the second elastic piece is in circumferential abutting connection with the puncture needle base (6).
5. The rapid detection biopsy puncture gun for benign and malignant tissue according to claim 1, characterized in that the handle portion (1) is detachably connected with the box body (2); the box body (2) is detachably connected with the sleeve (3).
6. The biopsy puncture gun for rapid detection of benign and malignant tissue according to claim 2, characterized in that a rotary plug (16) is arranged at one end of the handle part (1) far away from the puncture needle base (6), a plug groove (25) matched with the rotary plug (16) is arranged on the box body (2), and the plug groove (25) is communicated with the needle cavity (4).
7. The rapid detection biopsy puncture gun for benign and malignant tissue according to claim 1, wherein a waste liquid tank (26) is further arranged in the box body (2).
8. The rapid tissue wellness detection biopsy puncture gun of claim 1, wherein the injection port (241) has a height higher than the height of the port of the reaction well (22).
9. The rapid tissue wellness detection biopsy puncture gun according to claim 1, wherein the reaction tank (22) further comprises a grip portion (221), the grip portion (221) being located outside the cartridge (2).
10. The rapid tissue quality detection biopsy puncture gun according to claim 1, characterized in that the top of the box body (2) is made of transparent material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210164762.XA CN114533142B (en) | 2022-02-23 | 2022-02-23 | Biopsy puncture gun for rapidly detecting benign and malignant tissue |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210164762.XA CN114533142B (en) | 2022-02-23 | 2022-02-23 | Biopsy puncture gun for rapidly detecting benign and malignant tissue |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114533142A CN114533142A (en) | 2022-05-27 |
| CN114533142B true CN114533142B (en) | 2023-07-04 |
Family
ID=81677705
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210164762.XA Active CN114533142B (en) | 2022-02-23 | 2022-02-23 | Biopsy puncture gun for rapidly detecting benign and malignant tissue |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114533142B (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014084650A1 (en) * | 2012-11-30 | 2014-06-05 | 국립암센터 | Biotissue sampling apparatus |
| CN109758188A (en) * | 2019-02-19 | 2019-05-17 | 刘振宇 | A kind of clinical medicine biopsy sampling collection device |
| CN209332214U (en) * | 2018-11-01 | 2019-09-03 | 上海交通大学医学院附属第九人民医院 | A kind of cyst of jaw intervention sting device |
| JP2020039884A (en) * | 2019-11-13 | 2020-03-19 | シー・アール・バード・インコーポレーテッドＣ Ｒ Ｂａｒｄ Ｉｎｃｏｒｐｏｒａｔｅｄ | Biopsy device |
| CN110982518A (en) * | 2019-11-19 | 2020-04-10 | 上海应用技术大学 | Nitrogen-sulfur co-doped carbon quantum dot fluorescent probe for cysteine detection and preparation and application thereof |
| CN112075958A (en) * | 2019-06-13 | 2020-12-15 | 上海交通大学医学院附属第九人民医院 | Puncture sampling device |
| CN113855808A (en) * | 2021-09-08 | 2021-12-31 | 山西医科大学第二医院 | Application of nitrogen-doped carbon quantum dot delivery system in cartilage tissue |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5649547A (en) * | 1994-03-24 | 1997-07-22 | Biopsys Medical, Inc. | Methods and devices for automated biopsy and collection of soft tissue |
| WO2016105769A1 (en) * | 2014-12-23 | 2016-06-30 | Boston Scientific Scimed, Inc. | Marker for detection and confirmation of peripheral lung nodules |
| WO2018161067A1 (en) * | 2017-03-03 | 2018-09-07 | Arizona Board Of Regents On Behalf Of The University Of Arizona | Biopsy system for enhanced tissue harvesting |
-
2022
- 2022-02-23 CN CN202210164762.XA patent/CN114533142B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014084650A1 (en) * | 2012-11-30 | 2014-06-05 | 국립암센터 | Biotissue sampling apparatus |
| CN209332214U (en) * | 2018-11-01 | 2019-09-03 | 上海交通大学医学院附属第九人民医院 | A kind of cyst of jaw intervention sting device |
| CN109758188A (en) * | 2019-02-19 | 2019-05-17 | 刘振宇 | A kind of clinical medicine biopsy sampling collection device |
| CN112075958A (en) * | 2019-06-13 | 2020-12-15 | 上海交通大学医学院附属第九人民医院 | Puncture sampling device |
| JP2020039884A (en) * | 2019-11-13 | 2020-03-19 | シー・アール・バード・インコーポレーテッドＣ Ｒ Ｂａｒｄ Ｉｎｃｏｒｐｏｒａｔｅｄ | Biopsy device |
| CN110982518A (en) * | 2019-11-19 | 2020-04-10 | 上海应用技术大学 | Nitrogen-sulfur co-doped carbon quantum dot fluorescent probe for cysteine detection and preparation and application thereof |
| CN113855808A (en) * | 2021-09-08 | 2021-12-31 | 山西医科大学第二医院 | Application of nitrogen-doped carbon quantum dot delivery system in cartilage tissue |
Non-Patent Citations (1)
| Title |
|---|
| 分子影像与肝脏肿瘤诊治研究进展;王程远;王亚儒;唐海灵;;转化医学电子杂志(第02期);第58-62段 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114533142A (en) | 2022-05-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP4302517B2 (en) | In-situ inspection device adapter | |
| JP2019193868A (en) | System, instrument, and method for collecting body fluid sample | |
| US8846333B2 (en) | Method and device for visual detection of hemolysis | |
| KR20030011804A (en) | Method and device for sampling and analyzing interstitial fluid and whole blood samples | |
| JP4299675B2 (en) | Integrated storage adapter | |
| US11906410B2 (en) | Cell inspection device and cell inspection method | |
| US20180289329A1 (en) | Biological information detection device | |
| CN114533142B (en) | Biopsy puncture gun for rapidly detecting benign and malignant tissue | |
| JP2005073798A (en) | Puncture needle device for endoscope | |
| CN217408855U (en) | Novel puncture biopsy device | |
| CN214342330U (en) | Integrated fingertip blood sampling detection card | |
| KR102458777B1 (en) | Biological Fluid Collection and Stabilization Systems | |
| CN218331171U (en) | Intraoperative tissue benign and malignant rapid identification detection rod | |
| CN213430266U (en) | Intelligent biopsy needle | |
| JPH10501619A (en) | Swivel take-out plate | |
| Rossi et al. | Fine needle biopsy of thyroid nodules: the importance of technique | |
| CN217717766U (en) | Quick disease diagnosis device | |
| CN220757425U (en) | Biopsy needle | |
| CN218067657U (en) | Fecal occult blood detection reagent bottle | |
| CN217878915U (en) | Fecal occult blood detection reagent bottle | |
| CN218128553U (en) | Blood taking needle | |
| CN213489164U (en) | Novel puncture needle for nephrology department | |
| CN212540132U (en) | Device for detecting sperm concentration by near infrared spectrum | |
| CN212140452U (en) | A blood calcium detector for paracymeal patient that subtracts | |
| CN216622409U (en) | Glucometer data recorder |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |