CN114521508B - Lactobacillus is to intestinal mucosa's protective capacities simulation experiment device - Google Patents
Lactobacillus is to intestinal mucosa's protective capacities simulation experiment device Download PDFInfo
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- CN114521508B CN114521508B CN202210178193.4A CN202210178193A CN114521508B CN 114521508 B CN114521508 B CN 114521508B CN 202210178193 A CN202210178193 A CN 202210178193A CN 114521508 B CN114521508 B CN 114521508B
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- A—HUMAN NECESSITIES
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- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K29/00—Other apparatus for animal husbandry
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K29/00—Other apparatus for animal husbandry
- A01K29/005—Monitoring or measuring activity, e.g. detecting heat or mating
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention belongs to the field of lactobacillus experiments, and particularly relates to a lactobacillus intestinal mucosa protection capability simulation experiment device which comprises a device shell, wherein a fixed handle is welded and fixed on the top end surface of the device shell, a wireless camera device is installed on a bolt in the device shell, a connecting plate is welded and fixed on the top end of a reset spring, a connecting handle is welded and fixed on the top end of the connecting plate, an isolation feeding assembly is installed on the connecting plate, and an automatic driving assembly and a feeding and collecting assembly are installed in the device shell.
Description
Technical Field
The invention relates to the field of lactobacillus experiments, in particular to a protective capability simulation experiment device of lactobacillus for intestinal mucosa.
Background
According to previous researches, the lactobacillus can activate aromatic hydrocarbon receptors and induce indigenous layer lymphocytes to secrete IL-22, and the intestinal epithelial cell proliferation and replacement are critical to maintaining the structure of the intestinal mucosa, however, due to the lack of a proper in vitro model, the protection capability of the lactobacillus on the intestinal mucosa is still poorly known, so that a simulation experiment device is required to be combined for experiment, and the conventional simulation experiment device on the market has some problems in the use process;
the current phase of research suggests that the initial T cells (h 0) differentiate into three cell populations following activation: th1 type cells, th2 type cells and Treg cells, the activation of Th1 and Th2 cells are mutually inhibited, the differentiation of Th0 to Th1 and Th2 is mainly determined by cytokine environment at the initial sensitization, regulatory T cells are a T cell population which is different from Th1 and Th2 cells and has immunoregulation action, these cells are involved in the pathological process of multiple immune diseases, and mouse large intestine inflammation model experiment shows that under the stress of lactobacillus and pathogenic microorganism, lactobacillus interacts with host immune system to play a protective effect, the protective effect is realized by the immunoregulation action of T cells to a certain extent, including Thl and Th2 immune response reaction, jijng and others use lactobacillus as antigen expression vector research to discover that lactobacillus expressing Muramyl Dipeptide (MDP) and differentiation hormone can stimulate innate immune cells, including differentiation into helper T cells and regulatory T cells, inducing Th1 and Th17 to generate response and reducing response of Treg cells, and leading the lactobacillus to respond to Th1 cells and Treg cells by activating MAPK signal pathways, and further finding that lactobacillus clactobacillus reuteri) can cure diet-induced diabetes and obesity and stimulate Treg cell development by regulating CD4' Foxp3+ CD25 Treg cells, and the lactobacillus plays a role under different physiological and pathological conditions, generally plays a role in protecting intestinal mucosa barriers by balancing Th1/Th2 and inducing the development and release of relevant cytokines of Treg cells, while a conventional simulation experiment device needs to be combined with a plurality of devices to model, experiment and contrast mice in the using process, and the staff need feed the work one by one to the mouse in moulding, experiment and the contrast, work efficiency is low to conventional simulation experiment device is when examining the excrement and urine of mouse, and the inconvenient excrement and urine to the mouse is collected and is observed the processing work, and the practicality is relatively poor, consequently needs to provide a lactobacillus to satisfy user's demand to the protective capacities simulation experiment device of intestinal mucosa.
Disclosure of Invention
This section is for the purpose of summarizing some aspects of embodiments of the invention and to briefly introduce some preferred embodiments, and in this section as well as in the abstract and the title of the invention of this application some simplifications or omissions may be made to avoid obscuring the purpose of this section, the abstract and the title of the invention, and such simplifications or omissions are not intended to limit the scope of the invention.
The invention is provided in view of the problems of the existing lactobacillus in the intestinal mucosa protective capability simulation experiment device.
To solve the above technical problem, according to an aspect of the present invention, the present invention provides the following technical solutions: the utility model provides a lactobacillus is to intestinal mucosa's protective capacities simulation experiment device, includes the device shell, welded fastening has fixed handle on the top face of device shell, wireless camera equipment is installed to bolt in the device shell, welded fastening has reset spring on the top face of device shell, reset spring's top welded fastening has the linkage plate, the top welded fastening of linkage plate has the connection handle, install on the linkage plate and keep apart the subassembly of feeding, install in the device shell and drive the subassembly automatically and put in the collection subassembly.
As a preferred scheme of the lactobacillus protective capability simulation experiment device for intestinal mucosa, the invention comprises the following steps: the fixed handles are symmetrically distributed on two sides of the top end of the device shell, the reset spring is fixed in the middle of the bottom of the connecting plate, and the central axis of the connecting plate and the central axis of the device shell are located on the same vertical central line.
As a preferred scheme of the lactobacillus protective capability simulation experiment device for intestinal mucosa, the invention comprises the following steps: the subassembly is fed in the isolation includes the division board, feeds a section of thick bamboo, wheel ratch, circular gear, hob, a spacing section of thick bamboo, storage box and conveying pipeline, the top welded fastening of division board is on the bottom face of linking plate, feed a section of thick bamboo screw connection on the bottom side end face of division board, wheel ratch screw connection is on the middle part side end face of division board, circular gear meshing connects on the wheel ratch, circular gear welded fastening is on the top of hob, the hob passes through sealed bearing and rotates to be connected in a spacing section of thick bamboo and on the storage box, a spacing section of thick bamboo screw connection is on the inside bottom face of storage box, the top bolted connection of conveying pipeline is on the bottom face of a spacing section of thick bamboo, conveying pipeline bolted fastening is on the bottom face of storage box.
As a preferred scheme of the lactobacillus protective capability simulation experiment device for intestinal mucosa, the invention comprises the following steps: the baffle plate is symmetrically distributed on two sides of the connecting plate, the length of the baffle plate is greater than the height of the inner space of the device shell, the width of the baffle plate is equal to the width of the inner space of the device shell, and the feeding barrels are equidistantly distributed on the end face of the side of the bottom of the baffle plate.
As a preferred scheme of the lactobacillus protective capability simulation experiment device for intestinal mucosa, the invention comprises the following steps: the feeding barrel is in one-to-one correspondence with the conveying pipe through the limiting barrel, the central axis of the feeding barrel and the central axis of the conveying pipe are located on the same vertical central line, the diameter of the inner space of the limiting barrel is equal to that of the spiral rod, and the length of the spiral rod is larger than that of the inner space of the limiting barrel.
As a preferred scheme of the lactobacillus protective capability simulation experiment device for intestinal mucosa, the invention comprises the following steps: the automatic subassembly of driving is including dragging line, fixed coil, connecting axle, flexible brush and fixed frame, the top bolt that drags the line is on the bottom face of linkage plate, the bottom winding that drags the line is on fixed coil, fixed coil welded fastening is on the connecting axle, the connecting axle rotates to be connected in the device shell, flexible brush bolt is on the connecting axle, fixed frame welded fastening is in the device shell, welded fastening has the spiral spring in the fixed frame, the inner welded fastening of spiral spring is on the connecting axle.
As a preferred scheme of the lactobacillus protective capability simulation experiment device for intestinal mucosa, the invention comprises the following steps: the fixed coil is fixed in the middle part of connecting axle, flexible brush equidistance distributes on the connecting axle, fixed frame symmetric distribution is in the both sides of connecting axle, fixed frame and volute spiral spring one-to-one.
As a preferred scheme of the lactobacillus protective capability simulation experiment device for intestinal mucosa, the invention comprises the following steps: put in and collect the subassembly and include the intercommunication groove, protection door plant, first magnetic sheet, second magnetic sheet and collecting plate, the intercommunication groove runs through the bottom of seting up at the device shell, hub connection between the top of protection door plant and the device shell, the protection door plant sets up at the intercommunication inslot, first magnetic sheet screwed connection is in the bottom of protection door plant, first magnetic sheet adsorbs to be fixed on the second magnetic sheet, second magnetic sheet screwed connection is at the top of collecting plate, the spacing sliding connection of collecting plate is in the intercommunication inslot.
As a preferred scheme of the lactobacillus protective capability simulation experiment device for intestinal mucosa, the invention comprises the following steps: the width of the guard door plate and the width of the collecting plate are equal to the width of the inner space of the communicating groove, the length of the collecting plate is equal to the width of the device shell, the first magnetic plate and the second magnetic plate are respectively fixed at the middle part of the bottom of the guard door plate and the middle part of the top of the collecting plate, the shape and the size of the first magnetic plate are equal to the shape and the size of the second magnetic plate, and the cross section of the first magnetic plate is L-shaped.
Compared with the prior art, the invention has the beneficial effects that:
1. be provided with the isolation and feed the subassembly, the motion of utilizing the division board can carry out the moulding of integral type and follow-up isolated contrast simulation experiment work to the mouse, and the division board can drive circular gear through the teeth of a cogwheel pole and rotate, and then can drive the hob at spacing section of thick bamboo internal rotation, this moment under the rotation effect of hob, can carry the material equivalent of feeding in the storage box to each feeding section of thick bamboo in through the conveying pipeline, and then add work when can the various feeding materials of automatic stabilization completion, the use high efficiency of device has effectively been improved.
2. Be provided with the automatic subassembly of driveing, when the linkage plate drove the division board upward movement, it can drive the epaxial flexible brush rotation of connecting through fixed coil to drag the line, and then can drive the mouse after the success of moulding automatically to the avris observe the region in, and then can guarantee the stability and the convenience of follow-up contrast formula simulation experiment, increased the use variety and the stability of device.
3. Be provided with the collecting board, through the collecting board that uses the inside bottom of another collecting board thrust unit shell, move to the collecting board after the device shell is inside and the replacement use until another collecting board, change collecting board that can be convenient stable can effectively avoid the mouse to flee simultaneously, can carry out convenient stable collection work of taking to the excrement and urine of mouse in the experiment, and then can guarantee the observation accuracy and the work convenience of simulation experiment.
4. Be provided with the protection door plant, can carry out convenient stable the input and the work of taking to the mouse through rotating the protection door plant, the second magnetic sheet that the protection door plant used first magnetic sheet and collecting plate jointly simultaneously can accomplish jointly and adsorb fixed work, has increased the use variety and the high efficiency of device.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the present invention will be described in detail below with reference to the accompanying drawings and detailed embodiments, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without inventive exercise. Wherein:
FIG. 1 is a schematic diagram of the overall three-dimensional structure of a lactobacillus protective capability simulation experiment device for intestinal mucosa;
FIG. 2 is a schematic structural diagram of a protecting capability simulation experiment device of lactobacillus for intestinal mucosa at A in FIG. 1;
FIG. 3 is a schematic view of a three-dimensional structure of an isolation plate of a simulated experimental device for the protective capability of lactobacillus on intestinal mucosa;
FIG. 4 is a schematic perspective view of a collecting plate of the experimental device for simulating the protective capability of lactobacillus on intestinal mucosa;
FIG. 5 is a schematic perspective view of a protective door plate of the experimental device for simulating the protective capacity of lactobacillus on intestinal mucosa;
FIG. 6 is a schematic view of the overall structure of the experimental apparatus for simulating the protective ability of lactobacillus on intestinal mucosa according to the present invention;
FIG. 7 is a schematic structural view of a spiral rod of a lactobacillus protective capability simulation experiment device for intestinal mucosa;
FIG. 8 is a schematic view of a top view structure of a stationary coil of a lactobacillus intestinal mucosa protection ability simulation experiment device of the present invention;
FIG. 9 is a schematic structural view of a volute spring of a simulated experimental device for the protection capability of lactobacillus on intestinal mucosa;
FIG. 10 is a schematic view of a top-down structure of a limiting cylinder of an experimental device for simulating the protective capability of lactobacillus on intestinal mucosa;
fig. 11 is a schematic structural diagram of a protective capability simulation experiment device of lactobacillus for intestinal mucosa at a position B in fig. 10.
Reference numbers in the figures: 1. a device housing; 2. fixing a handle; 3. a wireless image pickup apparatus; 4. a return spring; 5. a connector tile; 6. a connecting handle; 7. isolating the feeding assembly; 701. a separator plate; 702. a feeding cylinder; 703. a pinion rod; 704. a circular gear; 705. a screw rod; 706. a limiting cylinder; 707. a storage box; 708. a delivery pipe; 8. an automatic driving assembly; 801. dragging the thread; 802. fixing the coil; 803. a connecting shaft; 804. a flexible brush; 805. a fixing frame; 9. a delivery collection assembly; 901. a communicating groove; 902. a protective door panel; 903. a first magnetic plate; 904. a second magnetic plate; 905. a collection plate; 10. a spiral spring.
Detailed Description
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in detail below.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, but the present invention may be practiced in other ways than those specifically described herein, and it will be apparent to those of ordinary skill in the art that the present invention may be practiced without departing from the spirit and scope of the present invention, and therefore the present invention is not limited to the specific embodiments disclosed below.
Next, the present invention is described in detail with reference to the drawings, and in the detailed description of the embodiments of the present invention, the cross-sectional view illustrating the structure of the device is not enlarged partially in general scale for the convenience of illustration, and the drawings are only exemplary, which should not limit the scope of the present invention. In addition, the three-dimensional dimensions of length, width and depth should be included in the actual fabrication.
Examples
In order to make the objects, technical solutions and advantages of the present invention more apparent, embodiments of the present invention will be described in detail with reference to the accompanying drawings.
As shown in fig. 1-11, a lactobacillus protective capability simulation experiment device for intestinal mucosa, including device shell 1, welded fastening has fixed handle 2 on the top face of device shell 1, wireless camera equipment 3 is installed to bolt in device shell 1, welded fastening has reset spring 4 on the top face of device shell 1, reset spring 4's top welded fastening has linkage plate 5, linkage plate 5's top welded fastening has connection handle 6, install on the linkage plate 5 and keep apart feeding subassembly 7, utilize to keep apart feeding subassembly 7 and can carry out the moulding of integral type and follow-up isolated contrast simulation experiment work to the mouse, can carry out automatic feeding work simultaneously to the mouse in a plurality of regions, simulation experiment's high efficiency and convenience have effectively been improved, install automatic driving subassembly 8 and put in collection subassembly 9 in the device shell 1.
In this example, fixed handle 2 symmetric distribution is in device shell 1 top both sides, and reset spring 4 is fixed in the bottom middle part of linkage plate 5, and the central axis of linkage plate 5 and the central axis of device shell 1 are located same vertical central line, can guarantee reset spring 4 on linkage plate 5 operating condition's stability, and then can guarantee the convenience and the stability of the follow-up reset work of linkage plate 5.
In this example, the isolation feeding assembly 7 includes an isolation plate 701, a feeding cylinder 702, a gear tooth rod 703, a circular gear 704, a screw rod 705, a limiting cylinder 706, a storage box 707, and a feeding pipe 708, wherein the top end of the isolation plate 701 is welded and fixed on the bottom end face of the connecting plate 5, the feeding cylinder 702 is screwed on the bottom side end face of the isolation plate 701, the gear tooth rod 703 is screwed on the middle side end face of the isolation plate 701, the circular gear 704 is meshed and connected on the gear tooth rod 703, the circular gear 704 is welded and fixed on the top end of the screw rod 705, the screw rod 705 is rotatably connected in the limiting cylinder 706 and the storage box 707 through a seal bearing, the limiting cylinder 706 is screwed on the inner bottom end face of the storage box 707, the top end of the feeding pipe 708 is bolted on the bottom end face of the limiting cylinder 706, the feeding pipe 708 is bolted on the bottom end face of the storage box 707, so that the working effect of the feeding pipe 708 on the limiting cylinder 706 and the storage box 707 can be stabilized, and the use diversity of the device can be increased.
In this example, the division board 701 symmetric distribution is in the both sides of linkage board 5, and the length of division board 701 is greater than the inner space height of device shell 1, and the width of division board 701 equals the inner space width of device shell 1, and feeding section of thick bamboo 702 equidistance distributes on the bottom side terminal surface of division board 701, can guarantee the stability of division board 701 work effect in device shell 1, and then can guarantee the follow-up stability of keeping apart observation work of mouse, has effectively improved the stability in utilization of device.
In this example, feeding cylinder 702 corresponds to conveying pipeline 708 one-to-one through spacing cylinder 706, the central axis of feeding cylinder 702 and the central axis of conveying pipeline 708 are located same vertical central line, the inside space diameter of spacing cylinder 706 is equal to the diameter of hob 705, the length of hob 705 is greater than the inside space length of spacing cylinder 706, the adverse effect of hob 705 on spacing cylinder 706 can be effectively avoided, and then the quantitative conveying work of follow-up feeding materials can be guaranteed, the use diversity of the device is increased.
In this example, the automatic driving assembly 8 includes a pulling line 801, a fixed coil 802, a connecting shaft 803, a flexible brush 804 and a fixed frame 805, the top end of the pulling line 801 is bolted to the bottom end face of the connecting plate 5, the bottom of the pulling line 801 is wound on the fixed coil 802, the fixed coil 802 is welded and fixed to the connecting shaft 803, the connecting shaft 803 is rotatably connected to the inside of the device housing 1, the flexible brush 804 is bolted to the connecting shaft 803, the fixed frame 805 is welded and fixed to the inside of the device housing 1, a scroll spring 10 is welded and fixed to the inside of the fixed frame 805, the inner end of the scroll spring 10 is welded and fixed to the connecting shaft 803, the scroll spring 10 can drive the connecting shaft 803 to perform convenient and stable rotation, and further, the stability and convenience of the connecting shaft 803 and the flexible brush 804 in subsequent multiple operations can be ensured.
In this example, the fixed coil 802 is fixed in the middle of the connecting shaft 803, the flexible brushes 804 are equidistantly distributed on the connecting shaft 803, the fixed frames 805 are symmetrically distributed on two sides of the connecting shaft 803, and the fixed frames 805 correspond to the volute springs 10 one to one, so that the stability of the working state of the flexible brushes 804 on the connecting shaft 803 can be ensured, and further the safety and the efficiency of the subsequent driving work of the mouse can be ensured.
In this example, put in and collect subassembly 9 including intercommunication groove 901, guard gate board 902, first magnetic plate 903, second magnetic plate 904 and collection board 905, intercommunication groove 901 runs through and sets up in the bottom of device shell 1, the top of guard gate board 902 and the device shell 1 intermediate shaft are connected, guard gate board 902 sets up in intercommunication groove 901, first magnetic plate 903 screwed connection is in guard gate board 902's bottom, first magnetic plate 903 adsorbs to be fixed on second magnetic plate 904, second magnetic plate 904 screwed connection is at the top of collection board 905, the spacing sliding connection of collection board 905 is in intercommunication groove 901, can guarantee first magnetic plate 903 in guard gate board 902 bottom operating condition's stability and safety, and then can guarantee the follow-up closed stable and convenient of opening work of guard gate board 902.
In this example, the width of guard gate 902 and the width of collecting plate 905 all equal with the inner space width of intercommunication groove 901, the length of collecting plate 905 equals with the width of device shell 1, first magnetic sheet 903 and second magnetic sheet 904 are fixed respectively at the bottom middle part of guard gate 902 and the top middle part of collecting plate 905, the shape size of stating first magnetic sheet 903 equals with the shape size of second magnetic sheet 904, the cross-section of first magnetic sheet 903 is "L" font, can guarantee that collecting plate 905 carries out stable spacing slip work in intercommunication groove 901, and then can guarantee that collecting plate 905 is follow-up to take the stability and the convenience of change work, increased the use convenience of device.
It should be noted that, the invention is a lactobacillus protective capability simulation experiment device for intestinal mucosa, firstly, a worker can pull a first magnetic plate 903 by using a hand to drive a guard door plate 902 to rotate upwards on a device shell 1, then the worker can equally put mice required for experiments into molding areas on two sides of the middle part formed by the device shell 1 and a partition plate 701, before the mice are put, the worker can pull an engaging plate 5 upwards by using a connecting handle 6 to drive partition plates 701 on two sides of the engaging plate 5 to move upwards simultaneously, at this time, under the action of the movement of the partition plate 701, the toothed bar 703 can be driven to move upwards, during the movement of the toothed bar, the spiral rod 703 on the circular gear 704 can be driven to stably rotate in a limiting cylinder 706 through the meshing connection with the circular gear 704, at this time, before the spiral rod 705 starts to rotate, no solution exists in the spiral rod 705, and when the spiral rod starts to rotate, DSS aqueous solution in a storage tank 707 is conveyed to the bottom of the spiral rod through the top of the spiral rod, and the equivalent amount of aqueous solution in the molding areas of the DSS feeding cylinder 701 can be conveyed to the storage area 702 in each molding area of the separating cylinder 701 through the spiral rod 705; as can be seen from fig. 1, 3 and 6, the device housing 1, in combination with the isolation plate 701, can form four isolation regions, two isolation regions in the middle are molding regions, and two isolation regions on two sides are an observation experiment region and a contrast observation region, respectively;
specifically, the lactobacillus in the storage tank 707 in the left experimental region formed by the device housing 1 and the partition plate 701 is equivalently delivered into each feeding cylinder 702 in the left experimental region, and similarly, the device housing 1 and the partition plate 701 form a right comparative observation region, and the physiological saline in the storage tank 707 in the right comparative observation region is equivalently delivered into each feeding cylinder 702 in the comparative observation region, when the partition plate 701 moves downwards and is reset, the circular gear 704 and the gear rack 703 can drive the spiral rod 705 to rotate in the opposite direction, and when the spiral rod 705 rotates in the opposite direction and is reset to the initial state, the spiral rod 705 reversely delivers the surplus DSS aqueous solution which is not delivered into the storage tank 707 until no solution exists in the spiral rod 707, and at this time, the spiral rod 705 returns to the initial position, and reciprocates in this way, so as to ensure the stability of the subsequent working state of the spiral rod 705;
then, a worker can observe a mouse in a molding area by using the wireless camera device 3, the mouse in the molding area can eat a DSS aqueous solution until the weight of the mouse is seriously reduced, the mouse is cacheless and has dull hair, thin and soft feces and non-shape feces appear on 3 rd day of an experiment and adhere to the periphery of an anus, bloody stool appears on 6 th day of the experiment, the molding success is proved, the duration is 7 days, in the molding process, the worker can push the collecting plate 905 at the bottom end in the device shell 1 by using the other collecting plate 905 until the other collecting plate 905 moves to the inside of the device shell 1 through the communicating groove 901 and replaces the used collecting plate 905, the collecting plate 905 can be replaced conveniently and stably, the escape of the mouse can be effectively avoided, and the feces of the mouse in the experiment can be collected and taken conveniently and stably; the communicating groove 901 is used for replacing the collecting plate 905;
in order to avoid the shaking and the shifting of the collecting plate 905 caused by the movement of the mouse, the collecting plate 905 can be conveniently and stably adsorbed and fixed with the first magnetic plate 903 on the guard door panel 902 by combining the second magnetic plate 904, so that the adsorption and fixing work of the guard door panel 902 and the collecting plate 905 can be completed together, and the stability of the working states of the guard door panel 902 and the collecting plate 905 can be ensured simultaneously;
when the model making of the mouse is successful, the worker can drive the connecting plate 5 to move upwards by continuously using the connecting handle 6, so that the partition plates 701 on the two sides of the connecting plate 5 can be driven to move upwards at the same time, the isolation and the intercommunication between the areas are opened, and the mouse can be automatically driven into the observation experiment area and the comparison observation area on the two sides by combining the flexible hairbrush 804;
specifically, at this moment, can drive under the motion effect of linkage plate 5 and involve line 801 upward movement, and under the motion effect of dragging line 801, can drive flexible brush 804 on the connecting axle 803 through fixed coil 802 and stably rotate, when division board 701 upward movement, the direction of rotation of flexible brush 804 on the connecting axle 803 of left side is clockwise, the direction of rotation of flexible brush 804 on the connecting axle 803 of right side is anticlockwise, therefore the flexible brush 804 of both sides all is the trend of outwards stirring, and then can carry out the work of driving of automatic stabilization to the mouse in the molding region, can drive the mouse in the molding region of both sides respectively to the experimental area and the contrast observation region of both sides, subsequently combine to use the interior flexible brush 10 of fixed frame scroll 805 to drive connecting axle 803 reverse rotation, can drive linkage plate 5 downward movement through the drive of fixed coil 802 and reset when dragging the line 801 rolling, and use reset spring 4 to further guarantee linkage plate 5 and reset the stability of work, subsequently staff's accessible uses collection board to collect the excrement and observe the mouse's in the experimental area and contrast observation region, and then can stably accomplish the intestinal canal experiment ability to the intestinal protection of the mouse in the contrast observation region.
While the invention has been described with reference to an embodiment, various modifications may be made and equivalents may be substituted for elements thereof without departing from the scope of the invention. In particular, the various features of the disclosed embodiments of the invention may be used in any combination, provided that no structural conflict exists, and the combinations are not exhaustively described in this specification merely for the sake of brevity and resource conservation. Therefore, it is intended that the invention not be limited to the particular embodiments disclosed, but that the invention will include all embodiments falling within the scope of the appended claims.
Claims (6)
1. A lactobacillus protective capability simulation experiment device for intestinal mucosa comprises a device shell (1) and is characterized in that; the device is characterized in that a fixed handle (2) is fixedly welded on the top end face of a device shell (1), a wireless camera device (3) is installed on a bolt in the device shell (1), a return spring (4) is fixedly welded on the top end face of the device shell (1), a connecting plate (5) is fixedly welded on the top end of the return spring (4), a connecting handle (6) is fixedly welded on the top end of the connecting plate (5), an isolation feeding assembly (7) is installed on the connecting plate (5), and an automatic driving assembly (8) and a throwing and collecting assembly (9) are installed in the device shell (1);
the isolation feeding assembly (7) comprises an isolation plate (701), a feeding cylinder (702), a gear tooth rod (703), a circular gear (704), a spiral rod (705), a limiting cylinder (706), a storage box (707) and a conveying pipeline (708), wherein the top end of the isolation plate (701) is fixedly welded on the bottom end face of the connecting plate (5), the feeding cylinder (702) is connected on the side end face of the bottom of the isolation plate (701) in a screw mode, the gear tooth rod (703) is connected on the side end face of the middle of the isolation plate (701) in a screw mode, the circular gear (704) is connected on the gear tooth rod (703) in a meshing mode, the circular gear (704) is fixedly welded on the top end of the spiral rod (705), the spiral rod (705) is rotatably connected in the limiting cylinder (706) and the storage box (707) through a sealing bearing, the limiting cylinder (706) is connected on the bottom end face of the inner portion of the storage box (707) in a screw mode, the top end of the conveying pipeline (708) is connected on the bottom end face of the limiting cylinder (706) in a bolt mode, and the storage box (707) is fixedly arranged on the storage box (708 in a bolt mode;
the automatic driving assembly (8) comprises a dragging line (801), a fixed coil (802), a connecting shaft (803), a flexible brush (804) and a fixed frame (805), wherein the top end of the dragging line (801) is bolted to the bottom end face of a connecting plate (5), the bottom of the dragging line (801) is wound on the fixed coil (802), the fixed coil (802) is welded and fixed on the connecting shaft (803), the connecting shaft (803) is rotationally connected into a device shell (1), the flexible brush (804) is bolted on the connecting shaft (803), the fixed frame (805) is welded and fixed into the device shell (1), a volute spiral spring (10) is welded and fixed into the fixed frame (805), and the inner end of the volute spiral spring (10) is welded and fixed onto the connecting shaft (803);
the throwing collection assembly (9) comprises a communicating groove (901), a protective door plate (902), a first magnetic plate (903), a second magnetic plate (904) and a collection plate (905), wherein the communicating groove (901) penetrates through the bottom of the device shell (1), the top of the protective door plate (902) is connected with a shaft between the device shell (1) through the shaft, the protective door plate (902) is arranged in the communicating groove (901), the first magnetic plate (903) is connected to the bottom of the protective door plate (902) through screws, the first magnetic plate (903) is fixed to the second magnetic plate (904) through adsorption, the second magnetic plate (904) is connected to the top of the collection plate (905) through screws, and the collection plate (905) is connected to the communicating groove (901) in a limiting sliding mode.
2. The lactobacillus protecting capability simulation experiment device for intestinal mucosa according to claim 1, wherein: the fixed handles (2) are symmetrically distributed on two sides of the top end of the device shell (1), the reset spring (4) is fixed in the middle of the bottom of the connecting plate (5), and the central axis of the connecting plate (5) and the central axis of the device shell (1) are located on the same vertical central line.
3. The experimental device for simulating the protective capability of lactobacillus on intestinal mucosa according to claim 2, is characterized in that: the separation plates (701) are symmetrically distributed on two sides of the connecting plates (5), the length of each separation plate (701) is larger than the height of the inner space of the device shell (1), the width of each separation plate (701) is equal to the width of the inner space of the device shell (1), and the feeding cylinders (702) are equidistantly distributed on the side end face of the bottom of each separation plate (701).
4. The experimental device for simulating the protective capability of lactobacillus on intestinal mucosa according to claim 3, wherein: the feeding cylinder (702) is in one-to-one correspondence with the conveying pipe (708) through a limiting cylinder (706), the central axis of the feeding cylinder (702) and the central axis of the conveying pipe (708) are located on the same vertical central line, the diameter of the inner space of the limiting cylinder (706) is equal to that of the screw rod (705), and the length of the screw rod (705) is greater than that of the inner space of the limiting cylinder (706).
5. The experimental device for simulating the protective capability of lactobacillus on intestinal mucosa according to claim 4, wherein: the fixed coil (802) is fixed in the middle of the connecting shaft (803), the flexible brushes (804) are distributed on the connecting shaft (803) at equal intervals, the fixed frames (805) are symmetrically distributed on two sides of the connecting shaft (803), and the fixed frames (805) correspond to the volute spiral springs (10) one by one.
6. The experimental device for simulating the protective capability of lactobacillus on intestinal mucosa according to claim 5, wherein: the width of guard gate board (902) and the width of collecting plate (905) all equal with the inner space width of intercommunication groove (901), the length of collecting plate (905) equals with the width of device shell (1), first magnetic sheet (903) and second magnetic sheet (904) are fixed respectively in the bottom middle part of guard gate board (902) and the top middle part of collecting plate (905), the shape size of first magnetic sheet (903) and the shape size of second magnetic sheet (904) equal, the cross-section of first magnetic sheet (903) personally submits "L" font.
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