CN114517159B - Biocontrol strain for preventing and controlling corn borers, application thereof and corn borer preventing and controlling bacterial agent - Google Patents
Biocontrol strain for preventing and controlling corn borers, application thereof and corn borer preventing and controlling bacterial agent Download PDFInfo
- Publication number
- CN114517159B CN114517159B CN202210139905.1A CN202210139905A CN114517159B CN 114517159 B CN114517159 B CN 114517159B CN 202210139905 A CN202210139905 A CN 202210139905A CN 114517159 B CN114517159 B CN 114517159B
- Authority
- CN
- China
- Prior art keywords
- aspergillus
- corn borer
- microbial inoculum
- nomius
- spiranthes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
- A01N63/34—Aspergillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Mycology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Virology (AREA)
- Botany (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biomedical Technology (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
A biocontrol strain for controlling corn borers, application thereof and a corn borer control microbial inoculum relate to a strain for biological control, application thereof and a pest control microbial inoculum. The strain is monascus (Aspergillus nomius) ACB1041.4, and is preserved in China Center for Type Culture Collection (CCTCC) No. M20211535. The strain for biologically preventing and controlling corn borers is used as an active ingredient of a corn borer preventing and controlling microbial inoculum. Strains from different geographies and hosts have certain host specialization, and the aspergillus rubellus (Aspergillus nomius) ACB1041.4 has excellent control effect on corn borers, has high temperature resistance and ultraviolet radiation resistance, can play a role in control in natural environment, and has a value of practical and wide application in fields.
Description
Technical Field
The invention relates to a strain for biological control, application thereof and a pest control microbial inoculum.
Background
Corn borer, also called corn borer, belongs to lepidoptera, the family of borer, mainly is harmful to corn, sorghum, millet, etc., can also be harmful to cotton sugarcane, sunflower, rice, beet, sugarcane beans, etc., and belongs to the world pest. The corn borer has the biggest harm to the corn, the harmful plant rate of the perennial spring corn is about 30 percent, the yield is reduced by 10 percent, the harmful plant rate of the summer corn can reach 90 percent, and the yield is generally reduced by 20 to 30 percent. The first hatched larva firstly takes mesophyll of tender leaves, the second-instar larva is harmful when concentrated in heart leaves, and the 3-4-instar larva bites other hard tissues.
Biological means are adopted to prevent and treat diseases and insect pests, but the existing biological control microorganism strains have the problems of poor temperature resistance and poor ultraviolet radiation resistance. Therefore, although the control effect is ideal in the laboratory, the method is difficult to be practically applied outdoors and under natural conditions.
Disclosure of Invention
The invention provides a strain for biologically preventing and controlling corn borers, application thereof and a corn borer preventing and controlling microbial inoculum.
The strain for biologically preventing and controlling corn borers is monascus (Aspergillus nomius) ACB1041.4, and is preserved in China Center for Type Culture Collection (CCTCC) with the preservation number of M20211535.
The strain for biologically preventing and controlling corn borers is applied as an active ingredient of a corn borer preventing and controlling bacterial agent, and the monascus purpureus (Aspergillus nomius) ACB1041.4 is applied as an active ingredient of the corn borer preventing and controlling bacterial agent.
The corn borer control microbial inoculum disclosed by the invention consists of aspergillus rubrum (Aspergillus nomius) ACB1041.4 spore suspension and a carrier dispersing agent.
Strains from different geographies and hosts have certain host specialization, and the aspergillus rubellus (Aspergillus nomius) ACB1041.4 has excellent control effect on corn borers, has high temperature resistance and ultraviolet radiation resistance, can play a role in control in natural environment, and has a value of practical and wide application in fields.
The aspergillus frasinensis (Aspergillus nomius) ACB1041.4 is aspergillus frasinensis; the preservation is carried out in China Center for Type Culture Collection (CCTCC), the preservation address is the university of Wuhan, the preservation number is CCTCC No. M20211535, and the preservation date is 2021, 12 months and 03 days.
Drawings
FIG. 1 is a mid-term colony morphology of A.spiranthes (Aspergillus nomius) ACB1041.4 of example 1 on PDA medium at 28 ℃;
FIG. 2 is a post colony morphology of A.spiranthes (Aspergillus nomius) ACB1041.4 of example 1 on PDA medium at 28 ℃;
FIG. 3 is a later microscopic image of A.spiranthes (Aspergillus nomius) ACB1041.4 of example 1 on PDA media at 28 ℃;
FIG. 4 is a phylogenetic tree of A.spiranthes (Aspergillus nomius) ACB1041.4 of example 1 constructed according to ITS ITS sequence.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The first embodiment is as follows: the strain for biologically preventing and controlling corn borers is monascus (Aspergillus nomius) ACB1041.4, and is preserved in China Center for Type Culture Collection (CCTCC) with the preservation number of M20211535.
In the process of investigating the overwintering base number of corn borers in a Xinjiang corn planting area (Xinjiang agricultural academy of sciences of Urufion, new year.4, N: 43.94, E: 87.46), obtaining the disease-bearing corn borers from the harvested corn stalks by a 'stalk splitting method', collecting the natural disease-bearing (morbid) overwintering corn borers, separating the overwintering natural disease-bearing (morbid) corn borers sample on an ultra-clean bench in a laboratory, picking part of mycelia from the overwintering corn borers with mycelia, inoculating to a PDA culture medium for separation culture, placing the flat plate in a constant temperature incubator at 28 ℃, when the mycelia cover half of the flat plate, picking up single colony under a microscope to see whether the mycelia are consistent with the fungi on the bodies, separating and purifying (the purified strain is transferred to an insect host to determine whether the strain is an ACgenic fungus), numbering 1041.4, and storing in a refrigerator at 4 ℃.
The isolated and preserved media were PDA media.
Morphological identification: strain ACB1041.4 was cultured on PDA at 28 ℃, the early colonies were white, yellow in the middle of the colonies after several days (middle period) and gradually spread out (as shown in fig. 1), green spots were continuously formed in the middle of the colonies after (late period) the whole colonies gradually spread to become green (as shown in fig. 2), and a large number of conidia or conidia clusters were produced (as shown in fig. 3).
Molecular identification: extracting genome DNA of the strain ACB1041.4 by using a CTAB method, amplifying ITS sequences by using fungus universal primers ITS1 and ITS4, sequencing amplified products, and obtaining an ITS sequencing result as shown in SEQ ID No.1. Comparing the ITS sequencing result with the gene sequences stored in the GanBank database, constructing a phylogenetic tree (shown in figure 4) by using MEGA 10.0 software, and identifying the strain ACB1041.4 as the monascus purpureus Aspergillus nomius by combining the morphology and named as the monascus purpureus (Aspergillus nomius) ACB1041.4.
Wherein, the fungus universal primer ITS1:5'-TCCGTAGGTGAACCTGCGG-3' and fungal universal primer ITS4: 5'-TCCTCCGCTTATTGATATGC-3'.
Strains of different geographical and host sources have a degree of host specialization and also differ in pathogenicity against target pests. The aspergillus ruber (Aspergillus nomius) ACB1041.4 of the embodiment has specificity and high efficiency for corn borers.
The second embodiment is as follows: the aspergillus rubrum (Aspergillus nomius) ACB1041.4 of the present embodiment is used as an active ingredient in a corn borer control bacterial agent.
And a third specific embodiment: the corn borer control microbial inoculum A of the embodiment consists of aspergillus rubus (Aspergillus nomius) ACB1041.4 spore suspension and a carrier dispersing agent; the carrier dispersant is 3% -5% of almond oil aqueous dispersion, 3% -5% of grape seed oil aqueous dispersion, 0.5% -1.5% of rhamnose solution, 0.01% -0.05% of ascorbic acid aqueous solution or 1% -2.5% of carboxymethyl cellulose aqueous solution.
The specific embodiment IV is as follows: the present embodiment differs from the third embodiment in that: the spore concentration of the Aspergillus spiranthes (Aspergillus nomius) ACB1041.4 in the Aspergillus spiranthes (Aspergillus nomius) ACB1041.4 spore suspension is 10 6 ～10 9 spores/mL; the volume ratio of the Aspergillus spiranthes (Aspergillus nomius) ACB1041.4 spore suspension to the carrier dispersant is 3:2. The other steps are the same as those of the third embodiment.
Fifth embodiment: the corn borer control microbial inoculum B of the embodiment consists of aspergillus rubus (Aspergillus nomius) ACB1041.4 spore suspension and a carrier dispersing agent; the carrier dispersant consists of two dispersants of almond oil aqueous dispersion with the concentration of 3% -5%, rhamnose solution with the concentration of 0.5% -1.5% and carboxymethyl cellulose aqueous solution with the concentration of 1% -2.5%.
Specific embodiment six: the present embodiment differs from the fifth embodiment in that: the spore concentration of the Aspergillus spiranthes (Aspergillus nomius) ACB1041.4 in the Aspergillus spiranthes (Aspergillus nomius) ACB1041.4 spore suspension is 10 6 ～10 9 spores/mL; the volume ratio of the aspergillus spiranthes (Aspergillus nomius) ACB1041.4 spore suspension to the carrier dispersing agent is 3:2; the volume ratio of the two dispersants in the carrier dispersant is 1:1. The other components are the same as those in the fifth embodiment.
Seventh embodiment: the corn borer control microbial inoculum C of the embodiment consists of aspergillus rubus (Aspergillus nomius) ACB1041.4 spore suspension and a carrier dispersing agent; the carrier dispersant consists of 3 to 5 percent of almond oil aqueous dispersion liquid, 0.5 to 1.5 percent of rhamnose solution and 1 to 2.5 percent of carboxymethyl cellulose aqueous solution.
Eighth embodiment: the present embodiment differs from the seventh embodiment in that: the spore concentration of the Aspergillus spiranthes (Aspergillus nomius) ACB1041.4 in the Aspergillus spiranthes (Aspergillus nomius) ACB1041.4 spore suspension is 10 6 ～10 9 spores/mL; the volume ratio of the aspergillus spiranthes (Aspergillus nomius) ACB1041.4 spore suspension to the carrier dispersing agent is 1:1; the volume ratio of the three dispersants in the carrier dispersant is 1:1:1. The other steps are the same as those in embodiment seven.
Example 1
According to the fourth embodiment, the corn borer control bacterial agents A1-A5 are prepared. Wherein the spore suspension of the Aspergillus spiranthes (Aspergillus nomius) ACB1041.4 is mixed with a carrier dispersant, and the spore concentration of the Aspergillus spiranthes (Aspergillus nomius) ACB1041.4 in the spore suspension of the Aspergillus spiranthes (Aspergillus nomius) ACB1041.4 is 10 8 spores/mL.
The carrier dispersing agent in the corn borer control microbial inoculum A1 is 3-5% of almond oil aqueous dispersion liquid;
the carrier dispersing agent in the corn borer control microbial inoculum A2 is grape seed oil aqueous dispersion with the concentration of 3-5 percent;
carrier dispersing agent in corn borer preventing and controlling microbial inoculum A3 is rhamnose solution with concentration of 0.5% -1.5%;
carrier dispersing agent in corn borer preventing and controlling microbial inoculum A4 is ascorbic acid water solution with concentration of 0.01% -0.05%;
the carrier dispersing agent in the corn borer control microbial inoculum A5 is carboxymethyl cellulose water solution with the concentration of 1% -2.5%.
According to a sixth embodiment, the corn borer control bacterial agents B1-B3 are prepared. Wherein, the spore suspension of the aspergillus spiranthes (Aspergillus nomius) ACB1041.4 is mixed with a carrier dispersing agent, and the spore concentration of the aspergillus spiranthes (Aspergillus nomius) ACB1041.4 in the spore suspension of the aspergillus spiranthes (Aspergillus nomius) ACB1041.4 is 2.5 gamma 10 8 spores/mL.
The carrier dispersing agent in the corn borer control microbial inoculum B1 consists of 3-5% of almond oil aqueous dispersion liquid and 0.5-1.5% of rhamnose solution;
the carrier dispersing agent in the corn borer control microbial inoculum B2 consists of 3-5% of almond oil aqueous dispersion liquid and 1-2.5% of carboxymethyl cellulose aqueous solution;
the carrier dispersing agent in the corn borer control microbial inoculum B3 consists of a carboxymethyl cellulose water solution with the concentration of 1-2.5 percent and a rhamnose solution with the concentration of 0.5-1.5 percent;
according to the eighth specific embodiment, the corn borer control microbial inoculum C1 is prepared. Wherein the spore suspension of the Aspergillus spiranthes (Aspergillus nomius) ACB1041.4 is mixed with a carrier dispersant, and the spore concentration of the Aspergillus spiranthes (Aspergillus nomius) ACB1041.4 in the spore suspension of the Aspergillus spiranthes (Aspergillus nomius) ACB1041.4 is 10 8 spores/mL.
The carrier dispersing agent in the corn borer control microbial inoculum C1 consists of 3-5% of almond oil aqueous dispersion liquid, 1-2.5% of carboxymethyl cellulose aqueous solution and 0.5-1.5% of rhamnose solution.
Control group: aspergillus spiranthes (Aspergillus nomius) ACB1041.4 spore suspension, wherein the spore concentration of Aspergillus spiranthes (Aspergillus nomius) ACB1041.4 is 2.5 gamma 10 8 spores/mL.
Spore germination rate and insecticidal activity experiment at normal temperature: the experimental results are shown in table 1.
TABLE 1
Temperature resistance experiment:
the spore suspension preparations are respectively filled in sterilized test tubes, sealed after being filled, respectively placed in water baths with different temperatures and heated for 30min at 30 ℃ and 40 ℃. The control group was not subjected to heat treatment, and 3 replicates were obtained for each group. 150. Mu.L, 2.8X10 were taken 2 The spore/mL spore suspension preparation is coated on a PDA culture medium plate, sealed and then placed in a culture environment of 26.0+/-1.0 ℃ for constant temperature culture for 48 hours, the colony numbers in each plate are counted, and the spore germination rate is calculated. The germination rate of the Aspergillus spiranthes (Aspergillus nomius) ACB1041.4 spores in each experimental group is in a decreasing trend along with the temperature rise, namely, the high temperature is not beneficial to the Aspergillus spiranthesSpore germination. Compared with the normal temperature, the spore germination rate of the control group is reduced by 15.87% and 29.36% at 30 ℃ and 40 ℃ in sequence, the accumulated death rate of the 7 th day is reduced by 10.92% and 21.83% respectively, and the LC50 and the LT50 are increased by different orders of magnitude. Compared with a control group, the carrier dispersing agent in the corn borer control microbial inoculum has a protective effect on the aspergillus rubrum (Aspergillus nomius) ACB1041.4 spores under the high temperature condition. The experimental results are shown in tables 2 to 3.
Table 2 (30 ℃ C.)
| Microbial inoculum | Spore germination rate% | Cumulative mortality at 7d (%) | LC50 | LT50 |
| A1 | 82.75 | 76.35 | 3.16×10 3 | 5.3 |
| A2 | 60.35 | 62.44 | 6.53×10 5 | 8.4 |
| A3 | 81.16 | 72.44 | 5.32×10 3 | 6.2 |
| A4 | 51.33 | 51.32 | 7.89×10 6 | 9.8 |
| A5 | 84.92 | 72.01 | 7.46×10 3 | 6.4 |
| B1 | 83.12 | 81.22 | 3.09×10 3 | 5.3 |
| B2 | 82.13 | 82.25 | 3.66×10 3 | 5.7 |
| B3 | 80.89 | 83.92 | 2.33×10 3 | 4.8 |
| C1 | 83.22 | 88.45 | 2.08×10 3 | 4.5 |
| Control group | 77.78 | 72.33 | 6.02×10 4 | 6.8 |
Table 3 (40 ℃ C.)
Uv resistance experiments:
after each experimental group was treated with 254nm ultraviolet lamp for 3min, the spore germination rate of each experimental group showed a decreasing trend, but the spore germination rate of aspergillus presbyopic (Aspergillus nomius) ACB1041.4 remained very high. As shown in Table 4, the carrier dispersant in the corn borer control microbial inoculum of the invention also plays a role in protecting the Aspergillus spiranthes (Aspergillus nomius) ACB1041.4 spores under the condition of ultraviolet irradiation.
TABLE 4 Table 4
| Microbial inoculum | Spore germination rate% | Cumulative mortality at 7d (%) | LC50 | LT50 |
| A1 | 78.19 | 60.22 | 4.67×10 4 | 5.8 |
| A2 | 47.66 | 48.01 | 9.73×10 6 | 10.2 |
| A3 | 70.23 | 57.33 | 5.34×10 4 | 6.2 |
| A4 | 45.44 | 42.09 | 7.88×10 7 | 9.6 |
| A5 | 55.56 | 58.44 | 7.94×10 5 | 7.3 |
| B1 | 76.33 | 74.55 | 3.26×10 4 | 5.9 |
| B2 | 68.73 | 74.89 | 3.85×10 4 | 6.8 |
| B3 | 64.34 | 73.22 | 5.01×10 4 | 6.2 |
| C1 | 81.22 | 83.98 | 2.54×10 4 | 5.2 |
| Control group | 54.90 | 53.78 | 7.99×10 5 | 8.7 |
Sequence listing
<110> institute of plant protection at academy of agricultural sciences in Xinjiang
<120> biocontrol strain for preventing and controlling corn borer, application thereof and corn borer preventing and controlling microbial inoculum
<141> 2022-02-16
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 539
<212> DNA
<213> A.Honda (Aspergillus nomius)
<400> 1
acccaacctc ccacccgtgt ttactgtacc ttagttgctt cggcgggccc gccgcaaggc 60
cgccgggggg catccgcccc cgggcccgcg cccgccggag acaccacgaa ctctgaacga 120
tctagtgaag tctgagttga ttgtatcgca atcagttaaa actttcaaca atggatctct 180
tggttccggc atcgatgaag aacgcagcga aatgcgataa ctagtgtgaa ttgcagaatt 240
ccgtgaatca tcgagtcttt gaacgcacat tgcgccccct ggtattccgg ggggcatgcc 300
tgtccgagcg tcattgctgc ccatcaagca cggcttgtgt gttgggtcgt cgtccccccc 360
tccggggggg gacgggccct aaaggcagcg gcggcaccgc gtccgatcct cgagcgtatg 420
gggctttgtc acccgctctg taggcccggc cggcgcttgc cgaacgcaaa acaaccattc 480
tttccaggtt gacctcggat caggtaggga tacccgctga acttaagcaa tcaaaatcc 539
Claims (8)
1. A biocontrol strain for preventing and controlling corn borer is prepared from red ribbon aspergillusAspergillus nomius) ACB1041.4 is preserved in China Center for Type Culture Collection (CCTCC) No. M20211535.
2. The use of the biocontrol strain for controlling corn borers according to claim 1 as an active ingredient of a corn borer control microbial inoculum.
3. A corn borer control microbial inoculum is characterized in that the corn borer control microbial inoculum is prepared from the spiranthes sinensis as defined in claim 1Aspergillus nomius) Spore suspension and carrier of ACB1041.4A bulk dispersant composition; the carrier dispersant is 3% -5% of almond oil aqueous dispersion, 3% -5% of grape seed oil aqueous dispersion, 0.5% -1.5% of rhamnose solution, 0.01% -0.05% of ascorbic acid aqueous solution or 1% -2.5% of carboxymethyl cellulose aqueous solution.
4. The corn borer control microbial inoculum according to claim 3, characterized in that aspergillus rubens is @ oAspergillus nomius) Aspergillus spiranthes in spore suspension of ACB1041.4Aspergillus nomius) Spore concentration of ACB1041.4 of 10 6 ~10 9 spores/mL; aspergillus spiranthesAspergillus nomius) The volume ratio of spore suspension of ACB1041.4 to carrier dispersant was 3:2.
5. A corn borer control microbial inoculum is characterized in that the corn borer control microbial inoculum is prepared from the spiranthes sinensis as defined in claim 1Aspergillus nomius) The spore suspension of ACB1041.4 is composed of carrier dispersant; the carrier dispersing agent consists of two dispersing agents of 3% -5% of almond oil aqueous dispersion, 0.5% -1.5% of rhamnose solution and 1% -2.5% of carboxymethyl cellulose aqueous solution.
6. The corn borer control microbial inoculum according to claim 5, characterized in that aspergillus rubens is @ oAspergillus nomius) Aspergillus spiranthes in spore suspension of ACB1041.4Aspergillus nomius) Spore concentration of ACB1041.4 of 10 6 ~10 9 spores/mL; aspergillus spiranthesAspergillus nomius) The volume ratio of the spore suspension of ACB1041.4 to the carrier dispersant is 3:2; the volume ratio of the two dispersants in the carrier dispersant is 1:1.
7. A corn borer control microbial inoculum is characterized in that the corn borer control microbial inoculum is prepared from the spiranthes sinensis as defined in claim 1Aspergillus nomius) The spore suspension of ACB1041.4 is composed of carrier dispersant; the carrier dispersant consists of 3% -5% of almond oil aqueous dispersion, 0.5% -1.5% of rhamnose solution and 1% -2.5% of carboxymethylAqueous base cellulose solution.
8. The corn borer control microbial inoculum according to claim 7, characterized in that aspergillus rubens is @ oAspergillus nomius) Aspergillus spiranthes in spore suspension of ACB1041.4Aspergillus nomius) Spore concentration of ACB1041.4 of 10 6 ~10 9 spores/mL; aspergillus spiranthesAspergillus nomius) The volume ratio of the spore suspension of ACB1041.4 to the carrier dispersant is 1:1; the volume ratio of the three dispersants in the carrier dispersant is 1:1:1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210139905.1A CN114517159B (en) | 2022-02-16 | 2022-02-16 | Biocontrol strain for preventing and controlling corn borers, application thereof and corn borer preventing and controlling bacterial agent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210139905.1A CN114517159B (en) | 2022-02-16 | 2022-02-16 | Biocontrol strain for preventing and controlling corn borers, application thereof and corn borer preventing and controlling bacterial agent |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114517159A CN114517159A (en) | 2022-05-20 |
| CN114517159B true CN114517159B (en) | 2023-06-23 |
Family
ID=81599499
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210139905.1A Active CN114517159B (en) | 2022-02-16 | 2022-02-16 | Biocontrol strain for preventing and controlling corn borers, application thereof and corn borer preventing and controlling bacterial agent |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114517159B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116926235B (en) * | 2023-09-18 | 2023-12-05 | 三亚中国检科院生物安全中心 | Fusarium RPA-CRISPR/Cas detection kit and method |
| CN117660202A (en) * | 2023-12-11 | 2024-03-08 | 云南省农业科学院农业环境资源研究所 | Aspergillus peak Aspergillus nomius and application thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2064952A1 (en) * | 2007-11-29 | 2009-06-03 | Bayer CropScience AG | Method for reducing mycotoxin contamination in maize |
| CN103561578A (en) * | 2011-05-26 | 2014-02-05 | 先正达参股股份有限公司 | Stabilized biocontrol water dispersible granules |
| CN104918493A (en) * | 2012-11-30 | 2015-09-16 | 拜尔农作物科学股份公司 | Ternary fungicidal and pesticidal mixtures |
| CN105451559A (en) * | 2012-12-18 | 2016-03-30 | 拜耳作物科学股份公司 | Binary fungicidal and bactericidal combinations |
| WO2017060499A1 (en) * | 2015-10-09 | 2017-04-13 | Bayer Cropscience Aktiengesellschaft | Use of pydiflumetofen for the reduction of mycotoxin contamination in plants |
| WO2020077042A1 (en) * | 2018-10-10 | 2020-04-16 | AgBiome, Inc. | Compositions and methods for controlling plant pests and improving plant health |
| CN111032683A (en) * | 2017-06-16 | 2020-04-17 | 先锋国际良种公司 | Insecticidal proteins from plants and methods of use thereof |
-
2022
- 2022-02-16 CN CN202210139905.1A patent/CN114517159B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2064952A1 (en) * | 2007-11-29 | 2009-06-03 | Bayer CropScience AG | Method for reducing mycotoxin contamination in maize |
| CN101969776A (en) * | 2007-11-29 | 2011-02-09 | 拜尔农作物科学股份公司 | Method for reducing mycotoxin contamination in maize |
| CN103561578A (en) * | 2011-05-26 | 2014-02-05 | 先正达参股股份有限公司 | Stabilized biocontrol water dispersible granules |
| CN104918493A (en) * | 2012-11-30 | 2015-09-16 | 拜尔农作物科学股份公司 | Ternary fungicidal and pesticidal mixtures |
| CN105451559A (en) * | 2012-12-18 | 2016-03-30 | 拜耳作物科学股份公司 | Binary fungicidal and bactericidal combinations |
| WO2017060499A1 (en) * | 2015-10-09 | 2017-04-13 | Bayer Cropscience Aktiengesellschaft | Use of pydiflumetofen for the reduction of mycotoxin contamination in plants |
| CN111032683A (en) * | 2017-06-16 | 2020-04-17 | 先锋国际良种公司 | Insecticidal proteins from plants and methods of use thereof |
| WO2020077042A1 (en) * | 2018-10-10 | 2020-04-16 | AgBiome, Inc. | Compositions and methods for controlling plant pests and improving plant health |
| CN113163771A (en) * | 2018-10-10 | 2021-07-23 | 农业生物群落股份有限公司 | Compositions and methods for controlling plant pests and improving plant health |
Non-Patent Citations (3)
| Title |
|---|
| Molecular identification and efficacy of entomopathogenic fungi isolates against larvae of the Asian corn borer Ostrinia furnacalis (Lepidoptera: Crambidae) in Xinjiang, China;Xiaowu Wang等;J Appl Microbiol.;第133卷;2979-2992 * |
| 新疆荒漠绿洲区亚洲玉米螟越冬幼虫4种主要病原真菌生物学特性及致病力测定;王小武等;中国生物防治学报;第39卷(第02期);305-312 * |
| 新疆荒漠绿洲区越冬亚洲玉米螟病原真菌种类 鉴定及多样性分析;蒋旭东等;中国生物防治学报;第38卷(第1期);18-28 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114517159A (en) | 2022-05-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN114517159B (en) | Biocontrol strain for preventing and controlling corn borers, application thereof and corn borer preventing and controlling bacterial agent | |
| US20220369648A1 (en) | Endophytic falciphora oryzae fo-r20 and its application | |
| CN112143673B (en) | Pseudomonas aurantiaca CM-6 and application thereof | |
| CN107365710B (en) | Metarhizium strain with strong pathogenicity on hazelnut weevil and application thereof | |
| CN114921347B (en) | Biocontrol bacterium for preventing and treating soybean pseudo-stalk seedling rot and application thereof | |
| CN110923154B (en) | Beauveria bassiana bh001 for preventing and treating quercus pernyi pests and application thereof | |
| CN112143658A (en) | Beauveria bassiana strain MQ-08 and application and microbial preparation thereof | |
| CN110129212B (en) | Aspergillus flavus PEAS-10 without producing aflatoxin and application thereof | |
| CN110074140B (en) | Biocontrol microbial inoculum for producing aspergillus flavus, preparation method and application thereof | |
| CN104195050A (en) | Lecanicillium attenuatum and application of lecanicillium attenuatum to control of crop nematodes and bemisia tabaci | |
| CN112375688A (en) | Beauveria bassiana and application thereof in biological prevention and control of corn northern leaf blight | |
| CN111662829B (en) | Metarhizium anisopliae CHMA-005 and application thereof in prevention and control of tea geometrid | |
| CN110894469B (en) | Metarhizium anisopliae strain with high pathogenicity on orange belt mythimna separata larvae and application thereof | |
| CN113061559A (en) | Composite biological agent and application thereof in preventing and treating rice blast and promoting rice growth | |
| CN116784353B (en) | Streptomyces nojirimensis strain and application of fermentation liquor thereof in inhibiting agaricus bisporus verrucaria resisting prochloraz manganese salt | |
| CN111808888A (en) | Chinese fir endophytic fungi fermentation filtrate and extract thereof, and preparation method and application of Chinese fir endophytic fungi fermentation filtrate and extract | |
| CN114292759B (en) | Fusarium oxysporum with function of preventing and treating tobacco continuous cropping obstacle | |
| CN114606140B (en) | Isaria fumosorosea and application thereof in controlling diaphorina citri | |
| Wen et al. | Bacillus subtilis subsp. spizizenii MB29 controls alfalfa root rot caused by Fusarium semitectum | |
| CN112143674B (en) | Pseudomonas aurantiaca CM-9 and application thereof | |
| CN112940948A (en) | Trichoderma longibrachiatum and application thereof | |
| CN112143675B (en) | Pseudomonas aurantiaca CM-8 and application thereof | |
| CN108192852A (en) | One plant of Bacillus subtillis GB519 for promoting paddy growth and antagonism Pyricularia oryzae and application | |
| CN114958622B (en) | Metarhizium anisopliae and application thereof in preventing and treating tea lissajous | |
| CN112143672B (en) | Pseudomonas aurantiaca CM-7 and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |