CN114509396B - Marine plankton luminescence measurement and recognition device - Google Patents
Marine plankton luminescence measurement and recognition device Download PDFInfo
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- CN114509396B CN114509396B CN202210413004.7A CN202210413004A CN114509396B CN 114509396 B CN114509396 B CN 114509396B CN 202210413004 A CN202210413004 A CN 202210413004A CN 114509396 B CN114509396 B CN 114509396B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A90/00—Technologies having an indirect contribution to adaptation to climate change
- Y02A90/30—Assessment of water resources
Abstract
The invention discloses a device for measuring and identifying marine plankton luminescence, which belongs to the technical field of spectral measurement of marine plankton and comprises the following components: an integrating sphere; a water inlet pipe having an inner surface coated with a light absorbing layer, the water inlet pipe having at least one bent portion; the inner surface of the water outlet pipe is coated with a light absorption layer, and the water outlet pipe is provided with at least one bent part; a flow meter; a perturbation device; a processing module; a light collection assembly, comprising: the optical fiber dispersion device comprises a first optical fiber, a baffle plate, a dispersion device and a photomultiplier tube array; the processing module calculates a bioluminescence dynamics hyperspectral curve in the water body according to the electric signals sent by the photomultiplier array, calculates bioluminescence intensity, and matches and identifies the luminous organisms. This marine plankton luminous measurement and recognition device through effectively shielding outside natural light interference and adopting gain adjustable photomultiplier, effectively improves bioluminescence detection precision and reliability.
Description
Technical Field
The invention belongs to the technical field of spectral component measurement and analysis, and particularly relates to a device for measuring and identifying marine plankton luminescence.
Background
There are approximately 700 luminescent organisms in the ocean, which belong to 16 phyla, with a size distribution ranging from single cells to large vertebrates, such as bacteria, dinoflagellates, radioactively, ctenopharyngodon, echinocysts, crustaceans, and the like. The photon mass of these luminescent organisms spans at least nine orders of magnitude, with significant differences in the luminescence time of different luminescent organisms. The marine plankton luminescence occupies an important position in marine bioluminescence due to multiple luminescence types and wide distribution. The plankton luminescence under the mechanical stimulation has important research significance and practical application value in the aspects of marine ecology and underwater target detection. In the aspect of marine ecology, the luminous intensity of underwater organisms changes along with the concentration of plankton and the change of the community structure of the organisms, so that the change characteristics of the plankton can be obtained by monitoring the bioluminescence, and the method has application value in many research fields such as marine ecology, establishment of marine ecological models, distinguishing of plankton populations and the like. The commonality of marine planktonic organisms with bioluminescence capability is that they produce light when mechanically stimulated, so in underwater target detection, the underwater bioluminescence phenomenon can provide a passive way to identify underwater light emitting sources.
With the development of the photoelectric technology in recent years, the luminescence of marine plankton is changed from qualitative to quantitative research. The typical bioluminescence measuring instrument is designed by the institute of oceanography (PML) of Primulus British, and is designed by a horizontal flow tube and a large-size photodiode, so that the measuring sensitivity is high. However, this device also has the following disadvantages: (1) the horizontal water inlet pipe is easy to be influenced by ambient light; (2) the dynamic range cannot be adjusted by adopting the photodiode; (3) only the luminous intensity can be detected, and the spectrum cannot be measured; (4) the instrument cannot be calibrated in real time.
The above information disclosed in this background section is only for enhancement of understanding of the background of the application and therefore it may comprise prior art that does not constitute known to a person of ordinary skill in the art.
Disclosure of Invention
The invention provides a device for measuring and identifying marine plankton luminescence, which aims to solve the technical problems that a marine plankton luminescence measuring device in the prior art is easily influenced by ambient light, measurement parameters are limited and the like.
In order to realize the purpose of the invention, the invention is realized by adopting the following technical scheme:
A marine plankton luminescence measurement and identification device, comprising:
the integrating sphere is internally provided with a spherical inner cavity, the integrating sphere is provided with a water inlet and a water outlet which are communicated with the inner cavity, and the wall of the inner cavity is coated with a diffuse reflection layer;
the inner surface of the water inlet pipe is coated with a light absorption layer, the water inlet pipe is provided with at least one bending part, and the water inlet pipe is fixedly connected with the integrating sphere and communicated with the water inlet;
the inner surface of the water outlet pipe is coated with a light absorption layer, the water outlet pipe is provided with at least one bent part, and the water inlet pipe is fixedly connected with the integrating sphere and is communicated with the water outlet;
the flow meter is arranged in the water outlet pipe;
the disturbance device is arranged at the communication position of the water inlet pipe and the integrating sphere and is used for generating mechanical vibration to stimulate plankton to emit light;
a processing module;
a light collection assembly, comprising:
the first optical fiber is connected with the integrating sphere, and the light incidence end of the first optical fiber penetrates through the integrating sphere and is inserted into the inner cavity;
the baffle is fixed in an inner cavity of the integrating sphere and shields the light incidence end, a gap is formed between the baffle and the wall of the inner cavity, and part of light diffusely reflected by the wall of the inner cavity is reflected to the light incidence end through the gap and enters the first optical fiber;
A dispersive device connected to the first optical fiber;
the photomultiplier array is arranged at the rear end of the dispersion device and used for converting the light emitted by the dispersion device into an electric signal and sending the electric signal to the processing module, and the gain of the photomultiplier array is adjustable;
the processing module calculates a bioluminescence dynamics spectral curve in the water body according to the electric signals sent by the photomultiplier tube array, calculates bioluminescence intensity, matches the bioluminescence dynamics spectral curve and the bioluminescence intensity with a bioluminescence biological characteristic spectral library, and identifies a luminous organism through matching.
In some embodiments of the invention, the perturbation device comprises an impeller and a motor, and the motor drives the impeller to rotate.
In some embodiments of the invention, the perturbation device comprises an impeller and a motor, and the motor drives the impeller to rotate.
In some embodiments of the invention, the impeller is arranged at the communication position of the water inlet pipe and the integrating sphere, and the impeller drives the surrounding water body to vibrate when rotating so as to stimulate plankton to emit light.
In some embodiments of the present invention, a water pump is disposed in the water outlet pipe, and is configured to discharge water in the inner cavity through the water outlet pipe.
In some embodiments of the present invention, a filter screen is disposed at the water inlet port of the water inlet pipe.
In some embodiments of the present invention, a baffle is disposed in front of the light incident end to block direct incident light of the light-emitting organism and measure diffuse reflected light of the integrating cavity.
In some embodiments of the present invention, the bioluminescence kinetic spectral curve is a bioluminescence intensity curve corresponding to a certain time interval, and the bioluminescence kinetic spectral curve
The calculating method comprises the following steps:
wherein the content of the first and second substances,
for the system scaling factor, g is the gain factor of the photomultiplier array,
for the luminescence center wavelength, V is the reading of the photomultiplier array, V d Is the dark current reading of the photomultiplier tube array;
bioluminescence intensity
The calculation method comprises the following steps:
In some embodiments of the present invention, the integrating sphere is further provided with a calibration port, the calibration port is communicated with the inner cavity, and the device for measuring and identifying marine plankton luminescence further comprises:
and one end of the second optical fiber is inserted into the calibration port and is hermetically connected with the calibration port, and the other end of the second optical fiber is arranged towards the standard irradiance lamp.
In some embodiments of the present invention, the method for determining the system calibration coefficient of the marine plankton luminescence measurement and identification device comprises:
turning on a standard irradiance lamp, and transmitting the emitted light to the inner cavity through a second optical fiber;
obtaining corresponding readings of the photomultiplier tube array at different gain coefficients g
；
Obtaining dark current readings of the photomultiplier array at different gain factors g
；
Calculating system scaling coefficients at different gain coefficients from the known standard lamp irradiance:
where I is the standard lamp irradiance.
In some embodiments of the present invention, the method for establishing the spectrum library of the luminescence biological characteristics comprises:
and respectively measuring the luminous kinetic spectrum curves of a plurality of known luminous organisms, calculating the luminous center wavelength, the luminous intensity and the luminous duration time, and establishing a luminous biological characteristic spectrum library.
Compared with the prior art, the invention has the advantages and positive effects that:
according to the device for measuring and identifying the marine plankton luminescence, firstly, the inner surface of the integrating sphere is coated with the lightproof diffuse reflection layer, and the light absorption layers are coated in the water inlet pipe and the water outlet pipe, so that external natural light can be prevented from entering the water inlet pipe and the water outlet pipe from the side surface, and further prevented from reflecting to enter the integrating sphere. Set up the flexion respectively through inlet tube and outlet pipe, the light that outside natural light got into from the port portion of inlet tube and outlet pipe is sheltered from by the flexion reflection, avoids further transmitting to the integrating sphere in, and this scheme can effectively shield the interference of falling any probably production of outside natural light, introduces luminous biology and measures in the integrating sphere for bioluminescence detection precision is higher.
Secondly, this scheme is through setting up the integrating sphere, and its inner chamber is the spheroid, and scribbles the diffuse reflection layer on the inner chamber wall, can carry out diffuse reflection many times with the light that the biology sent out in the integrating sphere for the light that every position department reflected on the inner chamber wall is all even, and then the light that the optical collection subassembly was gathered does not receive its position to set up the influence.
Thirdly, through setting up the baffle in this scheme for shelter from the light incidence tip at first optic fibre, it is also not direct collection light that the illuminating organism launches, but gathers the light through inner chamber wall diffuse reflection, and diffuse reflection light gets into the light incidence tip through the gap between baffle and the inner chamber wall, makes light collection not receive the influence of illuminating organism position in the integrating sphere, and the reliability is high.
Finally, the scheme adopts a dispersion device and a photomultiplier array for measuring a high spectrum curve of bioluminescence in the integral cavity, so that the spectrum characteristic of the luminous biological identification is increased, and the accuracy of the luminous biological identification is improved. The photomultiplier has different gains, and can be automatically adjusted according to the bioluminescence intensity, so that the device can be applied to measurement of bioluminescence curves with different concentrations.
Other features and advantages of the present invention will become more apparent from the following detailed description of the invention when taken in conjunction with the accompanying drawings.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the embodiments are briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without creative efforts.
FIG. 1 is a schematic structural diagram of an embodiment of the marine plankton luminescence measurement and identification device according to the present invention;
FIG. 2 is a graph of a bioluminescence kinetic spectrum for an embodiment of the marine plankton luminescence measurement and identification device of the present invention;
FIG. 3 is a graph of the luminescence kinetics of a typical marine organism measured in one embodiment of the proposed marine plankton luminescence measurement and identification apparatus;
in the figure: 11. an integrating sphere; 110. an inner cavity; 12. a water inlet pipe; 121. a first curved portion; 13. a water outlet pipe; 131. a second curved portion; 14. a flow meter; 151. a first light ray; 152. a baffle plate; 153. a dispersive device; 154. an array of photomultiplier tubes; 161. a turbine; 162. a motor; 17. a water pump; 18. filtering with a screen; 19. a calibration port; 20. a second optical fiber; 21. a standard irradiance lamp.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
It should be noted that in the description of the present invention, the terms of direction or positional relationship indicated by the terms "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. are based on the directions or positional relationships shown in the drawings, which are merely for convenience of description, and do not indicate or imply that the device or element must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance. In the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.
In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can, for example, be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
Example one
The present embodiment proposes a marine plankton luminescence measurement and identification device, as shown in fig. 1, including: integrating sphere 11, water inlet pipe 12, water outlet pipe 13, flow meter 14, processing module (not shown in the figure) and light collection assembly. The integrating sphere 11 has a spherical inner cavity 110 inside, the integrating sphere 11 has a water inlet and a water outlet communicated with the inner cavity 110, and the inner cavity 110 is coated with a diffuse reflection layer on its wall.
The internal surface coating of inlet tube 12 has the light absorption layer, and inlet tube 12 has at least one flexion, and for first flexion 121, inlet tube 12 and integrating sphere 11 fixed connection, and with the water inlet intercommunication, the internal surface coating of outlet pipe 13 has the light absorption layer, and outlet pipe 13 has at least one flexion, and for second flexion 131, inlet tube 12 and integrating sphere 11 fixed connection, and communicate with the delivery port.
The device for measuring and identifying the luminescence of the marine plankton is put in a water body when in use, and water in the water body enters the inner cavity 110 of the integrating sphere 11 from the water inlet pipe 12 under the action of water pressure or directional pumping pressure and is discharged through the water outlet pipe 13. The light-emitting organisms enter the inlet of the integral cavity along with water flow and are disturbed by the impeller, then enter the integral cavity to emit light, the emitted light is collected by the light collection assembly, and the processing module processes and analyzes the collected signals to identify the light-emitting organisms for quantitative research and analysis on water ecology, underwater target detection and the like.
In some embodiments of the invention, a flow meter 14 is provided in the outlet pipe 13 for detecting the speed of the water flow exiting through the outlet pipe 13.
In some embodiments of the present invention, the light collection assembly includes a first optical fiber 151, a baffle 152, a dispersion device 153, and an array of photomultiplier tubes 154.
The first optical fiber 151 is connected to the integrating sphere 11, and a light incident end of the first optical fiber 151 penetrates through the integrating sphere 11 and enters the inner cavity 110.
The baffle 152 is fixed in the inner cavity 110 of the integrating sphere 11 and shields the light incident end of the first optical fiber 151, a gap is formed between the baffle 152 and the inner cavity wall, and a part of light diffusely reflected by the inner cavity wall is reflected to the light incident end through the gap and enters the first optical fiber 151.
The dispersion device 153 is connected to the first optical fiber 151, and the photomultiplier array 154 is disposed at the rear end of the dispersion device 153, and is configured to convert the output light of the dispersion device 153 into an electrical signal and send the electrical signal to the processing module.
The processing module calculates a bioluminescence dynamics spectral curve in the water body according to the electric signals sent by the photomultiplier tube array 154, calculates bioluminescence intensity, matches the bioluminescence dynamics spectral curve and the bioluminescence intensity with a bioluminescence biological characteristic spectral library, and identifies the luminescent organisms by matching.
The luminous measuring and recognition device for marine plankton of this embodiment, at first, through the internal surface coating at integrating sphere 11 has the diffuse reflection layer of adiacticity, can prevent that outside natural light from getting into integrating sphere 11 in, through setting up the light-absorbing layer in inlet tube 12 and the outlet pipe 13, can prevent that outside natural light from getting into inlet tube 12 and outlet pipe 13 from the side, and then avoid outside natural light to be reflected and get into in integrating sphere 11. Set up the flexion respectively through inlet tube 12 and outlet pipe 13, the light that outside natural light got into from the port portion of inlet tube 12 and outlet pipe 13 is sheltered from by the flexion reflection, avoids outside natural light to penetrate to integrating sphere 11 directly, and this scheme can effectively shield the interference of any probably production of outside natural light, introduces luminous biology into integrating sphere 11 and measures for bioluminescence detection precision is higher.
Secondly, this scheme is through setting up integrating sphere 11, and its inner chamber 110 is the spheroid, and sets up the diffuse reflection layer on the inner chamber wall, can carry out diffuse reflection many times with the light that the biology sent in integrating sphere 11 for the light that every position department reflected on the inner chamber wall is all even, and then the light that the optical collection subassembly was gathered is not influenced by its position of setting up.
Thirdly, the baffle 152 is arranged in the scheme, so that the light incident end of the first optical fiber 151 is shielded, that is, the light emitted by the luminescent organisms is not directly collected, but the light diffusely reflected by the inner cavity wall is collected, and the diffusely reflected light enters the light incident end through the gap between the baffle 152 and the inner cavity wall, so that the light collection is not influenced by the positions of the luminescent organisms in the integrating sphere 11.
The principle of the device for measuring and identifying the marine plankton luminescence is that the luminescent plankton can emit light under mechanical stimulation, and the luminescence intensity, the central wavelength and the duration are related to the luminescent plankton, so that the luminescent plankton identification is carried out by measuring the bioluminescence intensity, the duration and the spectral range.
In some embodiments of the present invention, the first and second bent portions 121 and 131 may be L-shaped, U-shaped, S-shaped, m-shaped, w-shaped, or the like.
In this embodiment, as shown in fig. 1, the first bending portion 121 formed by the water inlet pipe 12 is U-shaped, and the second bending portion 131 formed by the water outlet pipe 13 is L-shaped.
In order to increase the probability that the device will capture the luminescence of the luminescent organisms, considering that some luminescent organisms will only emit light when they are stimulated by the outside world, in some embodiments of the invention the marine plankton luminescence measuring and identifying device further comprises a disturbing device, which is arranged in the water inlet pipe 12 and close to the water inlet, for disturbing the water entering the inner cavity 110. The luminescent organisms in the integrating sphere 11 emit light after being disturbed and stimulated, and then the light can be collected by the light collection assembly.
In some embodiments of the present invention, as shown in fig. 1, the perturbation device includes a turbine 161 and a motor 162, and the motor 162 can drive the turbine 161 to rotate. The disturbance is transmitted into the inner cavity 110 with the water flow, which in turn can stimulate the luminescent bioluminescence in the integrating sphere 11.
In some embodiments of the present invention, a water pump 17 is disposed in the water outlet pipe 13, and is used for discharging water in the inner cavity 110 through the water outlet pipe 13. And then under the effect of water pressure, outside water can be mended to integrating sphere 11 through inlet tube 12 in, can realize this marine plankton luminescence measurement and recognition device's continuous measurement and discernment.
In some embodiments of the present invention, a filter 18 is disposed at the water inlet port of the water inlet pipe 12 for blocking the entry of large plankton or other debris, preventing the water inlet pipe 12 or the water outlet pipe 13 from being blocked, and preventing the collection of bioluminescence from being interfered.
In some embodiments of the invention, the mesh has a pore size of 10 μm to 50 μm.
In some embodiments of the invention, the mesh size is 20 μm.
The light absorbing layers coated on the inner surfaces of the water inlet pipe 12 and the water outlet pipe 13 are preferably black, so that on one hand, external natural light can be prevented from entering the water pipe, and on the other hand, a small amount of entering light can be absorbed, reflection is prevented, and ambient light is prevented from entering the integrating cavity.
The diffuse reflection layer coated on the inner cavity wall can adopt titanium dioxide and polyurethane, and the reflectivity is more than 95% in the visible light range of 400nm-700 nm.
In some embodiments of the invention, the light incident end is disposed away from the water inlet and the water outlet. The influence of flowing water flow on light collection of the light collection assembly can be reduced.
In some embodiments of the present invention, the first optical fiber 151 is connected to a dispersion device 153 for spectral splitting, and the dispersion device 153 is typically implemented by a grating.
The dispersive device 153 is connected to the photomultiplier array 154 for receiving the spectral information of the bioluminescence. The response time of the photomultiplier array 154 is less than 0.01s, so that plankton luminescence dynamics curve measurement is realized; the photomultiplier is provided with 3-5 gains, so that high-precision measurement under different luminous intensities of different organisms is realized.
In some embodiments of the present invention, as shown in fig. 2, the bioluminescence kinetic spectral curve is a bioluminescence intensity curve corresponding to a certain time interval, in fig. 2, the horizontal axis is a time axis, the vertical axis is bioluminescence intensity, and the bioluminescence kinetic spectral curve is
The calculating method comprises the following steps:
wherein, the first and the second end of the pipe are connected with each other,
for the system scaling factor, g is the gain factor of the photomultiplier tube array 154,
for the luminescence center wavelength, V is the reading of the photomultiplier array 154, V d Is the dark current reading of the photomultiplier tube array 154.
Bioluminescence intensity
The calculation method comprises the following steps:
In some embodiments of the invention, the marine plankton luminescence measurement and identification device also has a calibration function.
As shown in fig. 1, the integrating sphere 11 is provided with a calibration port 19, the calibration port 19 is communicated with the inner cavity 110, the marine plankton luminescence measuring and identifying device further comprises a second optical fiber 20, one end of the second optical fiber 20 extends into the calibration port 19 and is hermetically connected with the calibration port 19, and the other end of the second optical fiber is arranged towards the standard irradiance lamp 21.
The targeting port 19 is used for system targeting, and the second optical fiber 20 is withdrawn from the targeting port 19 during normal instrument operation, and the targeting port 19 can be sealed by a sealing cap. The calibration port 19 is opened during calibration and a standard irradiance lamp beam is introduced by the second optical fiber 20 into the interior cavity 110.
In some embodiments of the present invention, the method for determining the system calibration coefficient of the marine plankton luminescence measurement and identification device comprises:
the standard irradiance lamp 21 is turned on and the emitted light is transmitted to the interior cavity 110 through the second optical fiber 20.
Obtaining readings corresponding to the photomultiplier tube array 154 at different gain factors g
。
Obtaining dark current readings for the photomultiplier tube array 154 at different gain factors g
。
Calculating system scaling coefficients at different gain coefficients from the known standard lamp irradiance:
system calibration can be obtained in the laboratory.
Where I is the standard lamp irradiance, a known quantity.
Through setting up the calibration port, can carry out real-time calibration, guarantee data quality, improve data reliability.
In some embodiments of the present invention, the method for establishing the spectrum library of the luminescence biological characteristics comprises:
and respectively measuring the luminous kinetic spectrum curves of a plurality of known luminous organisms, calculating the luminous center wavelength, the luminous intensity and the luminous duration time, and establishing a luminous biological characteristic spectrum library.
And establishing a luminous organism identification algorithm according to the luminous organism characteristic spectrum library, and determining the corresponding luminous organisms according to the identified information such as luminous intensity, central wavelength, duration and the like. As shown in fig. 3, to measure the luminescence kinetics curves of typical marine luminescent organisms, with different luminescence intensities and durations, the luminescence kinetics curves are stored in a library of luminescence biological characteristic spectra for comparison and identification of luminescent organisms in a target water body.
The above examples are only intended to illustrate the technical solution of the present invention, and not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes may be made and equivalents may be substituted for elements thereof; such modifications and substitutions do not depart from the spirit and scope of the corresponding claims.
Claims (7)
1. A marine plankton luminescence measurement and identification device, comprising:
the integrating sphere is internally provided with a spherical inner cavity, the integrating sphere is provided with a water inlet and a water outlet which are communicated with the inner cavity, and the wall of the inner cavity is coated with a diffuse reflection layer;
The inner surface of the water inlet pipe is coated with a light absorption layer, the water inlet pipe is provided with at least one bending part, and the water inlet pipe is fixedly connected with the integrating sphere and is communicated with the water inlet;
the inner surface of the water outlet pipe is coated with a light absorption layer, the water outlet pipe is provided with at least one bent part, and the water inlet pipe is fixedly connected with the integrating sphere and is communicated with the water outlet;
the flow meter is arranged in the water outlet pipe;
the disturbance device is arranged at the communication position of the water inlet pipe and the integrating sphere and is used for generating mechanical vibration to stimulate plankton to emit light;
a processing module;
a light collection assembly, comprising:
the first optical fiber is connected with the integrating sphere, and the light incidence end of the first optical fiber penetrates through the integrating sphere and is inserted into the inner cavity;
the baffle is fixed in the inner cavity of the integrating sphere and shields the light incidence end, a gap is formed between the baffle and the wall of the inner cavity, and part of light diffusely reflected by the wall of the inner cavity is reflected to the light incidence end through the gap and enters the first optical fiber;
a dispersive device connected to the first optical fiber;
the photomultiplier array is arranged at the rear end of the dispersion device and used for converting the light emitted by the dispersion device into an electric signal and sending the electric signal to the processing module, and the gain of the photomultiplier array is adjustable;
The processing module calculates a bioluminescence dynamics spectral curve in the water body according to the electric signals sent by the photomultiplier tube array, calculates bioluminescence intensity, matches the bioluminescence dynamics spectral curve and the bioluminescence intensity with a bioluminescence biological characteristic spectral library, and identifies a luminous organism through matching;
the baffle is arranged in front of the light incident end and used for blocking direct incident light of the luminous organisms and receiving diffuse reflected light of the integrating cavity;
the integrating sphere is provided with a calibration port, the calibration port is communicated with the inner cavity, and the marine plankton luminescence measurement and identification device further comprises:
one end of the second optical fiber extends into the calibration port and is hermetically connected with the calibration port, and the other end of the second optical fiber faces the standard irradiance lamp;
the bioluminescence dynamics spectrum curve is a bioluminescence intensity curve and a bioluminescence dynamics spectrum curve corresponding to a certain time interval
The calculation method comprises the following steps:
wherein the content of the first and second substances,
for the system scaling factor, g is the gain factor of the photomultiplier array,
for luminescence center wavelength, V is the photomultiplier array reading, and Vd is the dark current reading for the photomultiplier array;
Bioluminescence intensity
The calculation method comprises the following steps:
2. The marine plankton luminescence measurement and identification device of claim 1, wherein the perturbation device comprises an impeller and a motor, the motor driving the impeller to rotate.
3. The device for measuring and identifying marine plankton luminescence according to claim 2, wherein the impeller is disposed at the communication position of the water inlet pipe and the integrating sphere, and the impeller rotates to drive the surrounding water body to vibrate for stimulating plankton luminescence.
4. The marine plankton luminescence measurement and identification device of claim 1, wherein a water pump is disposed in the water outlet pipe for draining water in the internal cavity through the water outlet pipe.
5. The marine plankton luminescence measurement and identification device of claim 1, wherein a screen is provided at the water inlet port of the water inlet pipe.
6. The marine plankton luminescence measurement and identification device of claim 1, wherein the system calibration factor determination method of the marine plankton luminescence measurement and identification device is as follows:
Turning on a standard irradiance lamp, and transmitting the emitted light to the inner cavity through a second optical fiber;
obtaining readings corresponding to the photomultiplier tube array at different gain coefficients g
；
Obtaining different gain coefficients of the photomultiplier tube arrayDark current reading at g
；
Calculating system scaling coefficients at different gain coefficients from the known standard lamp irradiance:
where I is the standard lamp irradiance.
7. The marine plankton luminescence measurement and identification device of any one of claims 1-5, wherein the method for establishing the luminescence biological characteristic spectrum library is as follows:
and respectively measuring the luminous kinetic spectrum curves of a plurality of known luminous organisms, calculating the luminous center wavelength, the luminous intensity and the luminous duration time, and establishing a luminous biological characteristic spectrum library.
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Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
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