CN114504072A - Application of negative ion liquid in removing aflatoxin and method for removing feed aflatoxin - Google Patents
Application of negative ion liquid in removing aflatoxin and method for removing feed aflatoxin Download PDFInfo
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- CN114504072A CN114504072A CN202210157821.0A CN202210157821A CN114504072A CN 114504072 A CN114504072 A CN 114504072A CN 202210157821 A CN202210157821 A CN 202210157821A CN 114504072 A CN114504072 A CN 114504072A
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- Prior art keywords
- aflatoxin
- mixed solution
- liquid
- aqueous solution
- negative ion
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Links
- 229930195730 Aflatoxin Natural products 0.000 title claims abstract description 43
- 239000005409 aflatoxin Substances 0.000 title claims abstract description 43
- XWIYFDMXXLINPU-UHFFFAOYSA-N Aflatoxin G Chemical compound O=C1OCCC2=C1C(=O)OC1=C2C(OC)=CC2=C1C1C=COC1O2 XWIYFDMXXLINPU-UHFFFAOYSA-N 0.000 title claims abstract description 38
- 239000007788 liquid Substances 0.000 title claims abstract description 35
- 238000000034 method Methods 0.000 title claims abstract description 27
- 239000011259 mixed solution Substances 0.000 claims abstract description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 21
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims abstract description 20
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims abstract description 20
- 150000002500 ions Chemical class 0.000 claims abstract description 18
- 238000010438 heat treatment Methods 0.000 claims abstract description 16
- 150000001450 anions Chemical class 0.000 claims abstract description 15
- 238000002156 mixing Methods 0.000 claims abstract description 11
- 239000004115 Sodium Silicate Substances 0.000 claims abstract description 10
- 229910021538 borax Inorganic materials 0.000 claims abstract description 10
- 239000001103 potassium chloride Substances 0.000 claims abstract description 10
- 235000011164 potassium chloride Nutrition 0.000 claims abstract description 10
- 229910000029 sodium carbonate Inorganic materials 0.000 claims abstract description 10
- NTHWMYGWWRZVTN-UHFFFAOYSA-N sodium silicate Chemical compound [Na+].[Na+].[O-][Si]([O-])=O NTHWMYGWWRZVTN-UHFFFAOYSA-N 0.000 claims abstract description 10
- 229910052911 sodium silicate Inorganic materials 0.000 claims abstract description 10
- 239000004328 sodium tetraborate Substances 0.000 claims abstract description 10
- 235000010339 sodium tetraborate Nutrition 0.000 claims abstract description 10
- 235000000346 sugar Nutrition 0.000 claims abstract description 10
- 239000002994 raw material Substances 0.000 claims abstract description 9
- 235000017550 sodium carbonate Nutrition 0.000 claims abstract description 9
- 235000019794 sodium silicate Nutrition 0.000 claims abstract description 9
- 238000001816 cooling Methods 0.000 claims abstract description 8
- 230000001376 precipitating effect Effects 0.000 claims abstract description 6
- 239000007864 aqueous solution Substances 0.000 claims description 25
- 238000001556 precipitation Methods 0.000 claims description 4
- 239000000243 solution Substances 0.000 claims description 2
- 125000000129 anionic group Chemical group 0.000 claims 3
- 230000000694 effects Effects 0.000 abstract description 4
- 239000011265 semifinished product Substances 0.000 description 6
- 238000001914 filtration Methods 0.000 description 5
- 239000000047 product Substances 0.000 description 4
- 238000009924 canning Methods 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 2
- 238000001784 detoxification Methods 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- KWZYQHQNOWRQRG-UHFFFAOYSA-N 3beta-hydroxytremetone Natural products C1=C(C(C)=O)C=C2C(O)C(C(=C)C)OC2=C1 KWZYQHQNOWRQRG-UHFFFAOYSA-N 0.000 description 1
- 241000228197 Aspergillus flavus Species 0.000 description 1
- 241000228230 Aspergillus parasiticus Species 0.000 description 1
- 206010007269 Carcinogenicity Diseases 0.000 description 1
- KWZYQHQNOWRQRG-OLZOCXBDSA-N Toxol Chemical compound C1=C(C(C)=O)C=C2[C@@H](O)[C@H](C(=C)C)OC2=C1 KWZYQHQNOWRQRG-OLZOCXBDSA-N 0.000 description 1
- 239000002115 aflatoxin B1 Substances 0.000 description 1
- OQIQSTLJSLGHID-WNWIJWBNSA-N aflatoxin B1 Chemical compound C=1([C@@H]2C=CO[C@@H]2OC=1C=C(C1=2)OC)C=2OC(=O)C2=C1CCC2=O OQIQSTLJSLGHID-WNWIJWBNSA-N 0.000 description 1
- 229930020125 aflatoxin-B1 Natural products 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- AXCZMVOFGPJBDE-UHFFFAOYSA-L calcium dihydroxide Chemical compound [OH-].[OH-].[Ca+2] AXCZMVOFGPJBDE-UHFFFAOYSA-L 0.000 description 1
- 230000007670 carcinogenicity Effects 0.000 description 1
- 231100000260 carcinogenicity Toxicity 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 108700012359 toxins Proteins 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/27—Removal of unwanted matter, e.g. deodorisation or detoxification by chemical treatment, by adsorption or by absorption
- A23L5/276—Treatment with inorganic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/27—Removal of unwanted matter, e.g. deodorisation or detoxification by chemical treatment, by adsorption or by absorption
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Fodder In General (AREA)
Abstract
The application discloses application of a negative ion liquid in removing aflatoxin and a method for removing feed aflatoxin. The anion liquid is obtained by the following steps: mixing raw materials containing sodium carbonate, potassium chloride, sodium silicate, borax, sugar and water to obtain a mixed solution, heating the mixed solution, conveying the heated mixed solution to a storage filter tank, precipitating, and cooling to obtain the anion liquid. The application finds that the anion liquid has a good effect of clearing aflatoxin and has a good application prospect in clearing aflatoxin.
Description
Technical Field
The application relates to application of a negative ion liquid in removing aflatoxin, belonging to the technical field of feeds.
Background
Aflatoxins (AFT) are difuranocyclic toxoids produced by some strains of aspergillus flavus and aspergillus parasiticus. There are about 20 derivatives, named as B1, B2, G1, G2, M1, M2, GM, P1, Q1, and toxol. Among them, B1 has the highest toxicity and the highest carcinogenicity. Aflatoxins mainly contaminate grains and oils and their products, as well as various vegetable and animal foods. The aflatoxin is also a common pollutant in the feed, and trace toxins can be detected in the liver, kidney, muscle, blood, milk and eggs after animals eat the feed polluted by the aflatoxin. The feed with slight pollution can reach the feeding standard after proper treatment and can still be used, while the feed with severe aflatoxin pollution is probably discarded. The currently common methods for removing aflatoxin in feed mainly comprise: the method comprises a water washing method, a picking method, an exposure detoxification method, a degerming detoxification method, a lime water soaking method, a heat treatment method, an alkali boiling treatment method, a method of adding antibiotics into the feed and the like, but in general, the method solves the problem that aflatoxin in the feed is complex.
Disclosure of Invention
According to one aspect of the application, the application of the negative ion liquid in removing aflatoxin is provided. The application finds that the anion liquid has a good effect of clearing aflatoxin and has a good application prospect in clearing aflatoxin.
The application of the negative ion liquid in removing aflatoxin is characterized in that the negative ion liquid is obtained by the following steps:
mixing raw materials containing sodium carbonate, potassium chloride, sodium silicate, borax, sugar and water to obtain a mixed solution, heating the mixed solution, conveying the heated mixed solution to a storage filter tank, precipitating, and cooling to obtain the anion liquid.
Optionally, the heating time is 5 minutes or more.
Optionally, the heating temperature is 90-120 ℃;
the heating time is 5-30 minutes;
the precipitation time is 24-48 hours.
Optionally, the negative ion liquid is obtained by the following steps:
(1) adding water into a stirrer, adding the following raw materials, and uniformly mixing and stirring to obtain a mixed solution: sodium carbonate, potassium chloride, sodium silicate, borax, sugar and water;
(2) heating the mixed solution in the stirrer for more than 5 minutes;
(3) conveying the heated mixed solution to a storage and filtration tank through a water pump, and cooling the precipitate to room temperature to obtain a semi-finished product;
(4) and (4) taking the semi-finished product obtained in the step (3) out of the storage and filtration tank, and canning to obtain a finished product.
Optionally, in the step (1), the weight ratio of sodium carbonate, potassium chloride, sodium silicate, borax, sugar and water is 15-35: 1-20: 1-15: 1-13: 1-15: 2 to 81.
Optionally, in the step (2), the temperature during heating is 90-120 ℃; in the step (3), the precipitation time is 24-48 hours.
According to another aspect of the present application, there is provided a method of removing aflatoxins from feed, the method comprising the steps of:
dissolving the feed with aflatoxin in water to obtain an aqueous solution, mixing the aqueous solution with the negative ion solution, and standing to remove aflatoxin in the feed;
the anion liquid is obtained by the following steps:
mixing raw materials containing sodium carbonate, potassium chloride, sodium silicate, borax, sugar and water to obtain a mixed solution, heating the mixed solution, conveying the heated mixed solution to a storage filter tank, precipitating, and cooling to obtain the anion liquid.
Optionally, the concentration of aflatoxin in the aqueous solution is 8-22 μ g/ml.
Optionally, the concentration of aflatoxin in the aqueous solution is 11-16 μ g/ml.
Optionally, the concentration of aflatoxin in the aqueous solution is any one of or a range of values between 8 μ g/ml, 9 μ g/ml, 10 μ g/ml, 12 μ g/ml, 14 μ g/ml, 15 μ g/ml, 18 μ g/ml, 20 μ g/ml, 22 μ g/ml.
Optionally, the volume ratio of the aqueous solution to the negative ion liquid is 100: 0.5 to 7.
Optionally, the volume ratio of the aqueous solution to the negative ion liquid is 100: 0.8 to 5.
Optionally, the volume ratio of the aqueous solution to the negative ion liquid is 100: 0.8 to 3.
Optionally, the volume ratio of the aqueous solution to the negative ion liquid is 100: 0.5, 100: 0.8, 100: 1. 100, and (2) a step of: 2. 100, and (2) a step of: 3. 100: 4. 100, and (2) a step of: 5. 100, and (2) a step of: 6. 100, and (2) a step of: 7 or a range of values between any two values.
Optionally, the standing time is 3-8 hours.
Optionally, the standing time is 3-6 hours.
Optionally, the time of standing is any one of 3 hours, 4 hours, 5 hours, 6 hours, 7 hours, 8 hours or a range between any two values.
The beneficial effects that this application can produce include:
(1) the application creatively discovers that the anion liquid has a good effect of clearing aflatoxin and has a good application prospect in clearing aflatoxin.
(2) The method for removing the aflatoxin in the feed is simple to operate and high in removal rate.
Detailed Description
The present application will be described in detail with reference to examples, but the present application is not limited to these examples.
The raw materials in the examples of the present application were all purchased commercially, unless otherwise specified.
As an embodiment, the anion liquid used in the present application can be obtained by the following steps:
(1) adding water into a stirrer, adding the following raw materials, and uniformly mixing and stirring to obtain a mixed solution: sodium carbonate, potassium chloride, sodium silicate, borax, sugar and water;
(2) heating the mixed solution in the stirrer for more than 5 minutes;
(3) conveying the heated mixed solution to a storage and filtration tank through a water pump, and cooling the precipitate to room temperature to obtain a semi-finished product;
(4) and (4) taking the semi-finished product obtained in the step (3) out of the storage and filtration tank, and canning to obtain a finished product.
Further, in the step (1), the weight ratio of sodium carbonate, potassium chloride, sodium silicate, borax, sugar and water is 15-35: 1-20: 1-15: 1-13: 1-15: 2 to 81.
Further, in the step (2), the heating temperature is 90-120 ℃; in the step (3), the precipitation time is 24-48 hours.
As an implementation scheme, the feed containing aflatoxin is dissolved in water to prepare an aqueous solution, and the aqueous solution is mixed with the negative ion liquid and is placed for a period of time, so that the aflatoxin in the feed can be efficiently removed.
Further, the concentration of the aflatoxin in the aqueous solution is 8-22 mug/ml.
Further, the volume ratio of the aqueous solution to the negative ion liquid is 100: 0.5 to 7.
Further, the standing time is 3-8 hours.
Example 1 detection of Effect of anion liquid on removing aflatoxin in feed
(1) Preparation of anion liquid
The preparation method of the anion liquid refers to example 2 with the patent publication number of CN102239959A, and comprises the following specific steps:
(1.1) adding water into the stirrer, adding the following raw materials in percentage by weight, and then uniformly mixing and stirring to obtain a mixed solution: 35% of sodium carbonate, 20% of potassium chloride, 15% of sodium silicate, 13% of borax, 15% of sugar and 2% of water;
(1.2) heating the mixed solution in the stirrer for 8 minutes at the temperature of 120 ℃;
(1.3) conveying the heated mixed solution to a storage and filtration tank through a water pump, precipitating for 48 hours, and cooling to room temperature to obtain a semi-finished product;
and (1.4) taking the semi-finished product obtained in the step (1.3) out of the filter storage tank, and canning to obtain a finished product, which is recorded as a sample a.
(2) Determination of aflatoxin removal rate of negative ion liquid
The specific determination method comprises the following steps: putting the feed containing the aflatoxin into 1000ml of water to obtain an aqueous solution, and detecting the aflatoxin Bl concentration in the aqueous solution to be 13.40 mu g/ml by using an HPLC method; continuously adding 10ml of sample a (namely the volume ratio of the aqueous solution to the sample a is 100: 1) into 1000ml of the aqueous solution, and uniformly mixing; after standing for 4 hours, the removal rate of aflatoxin B1 in the aqueous solution was detected to be 91.54%.
Although the present application has been described with reference to a few embodiments, it should be understood that various changes, substitutions and alterations can be made herein without departing from the spirit and scope of the application as defined by the appended claims.
Claims (10)
1. The application of the negative ion liquid in removing aflatoxin is characterized in that the negative ion liquid is obtained by the following steps:
mixing raw materials containing sodium carbonate, potassium chloride, sodium silicate, borax, sugar and water to obtain a mixed solution, heating the mixed solution, conveying the heated mixed solution to a storage filter tank, precipitating, and cooling to obtain the anion liquid.
2. The use according to claim 1, wherein the temperature of the heating is 90 to 120 ℃;
the heating time is 5-30 minutes; the precipitation time is 24-48 hours.
3. A method for removing aflatoxins from feed, comprising the steps of:
dissolving the feed with aflatoxin in water to obtain an aqueous solution, mixing the aqueous solution with the negative ion solution, and standing to remove aflatoxin in the feed;
the anion liquid is obtained by the following steps:
mixing raw materials containing sodium carbonate, potassium chloride, sodium silicate, borax, sugar and water to obtain a mixed solution, heating the mixed solution, conveying the heated mixed solution to a storage filter tank, precipitating, and cooling to obtain the anion liquid.
4. The method according to claim 3, wherein the concentration of aflatoxin in the aqueous solution is 8-22 μ g/ml.
5. The method according to claim 3, wherein the concentration of aflatoxin in the aqueous solution is 11-16 μ g/ml.
6. The method of claim 3, wherein the volume ratio of the aqueous solution to the anionic liquid is 100: 0.5 to 7.
7. The method of claim 3, wherein the volume ratio of the aqueous solution to the anionic liquid is 100: 0.8 to 5.
8. The method of claim 3, wherein the volume ratio of the aqueous solution to the anionic liquid is 100: 0.8 to 3.
9. The method according to claim 3, wherein the standing time is 3 to 8 hours.
10. The method according to claim 3, wherein the standing time is 3 to 6 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210157821.0A CN114504072A (en) | 2022-02-21 | 2022-02-21 | Application of negative ion liquid in removing aflatoxin and method for removing feed aflatoxin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210157821.0A CN114504072A (en) | 2022-02-21 | 2022-02-21 | Application of negative ion liquid in removing aflatoxin and method for removing feed aflatoxin |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114504072A true CN114504072A (en) | 2022-05-17 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210157821.0A Pending CN114504072A (en) | 2022-02-21 | 2022-02-21 | Application of negative ion liquid in removing aflatoxin and method for removing feed aflatoxin |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114504072A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR7401040A (en) * | 1973-02-13 | 1975-11-04 | Battelle Memorial Institute | PROCESS TO REDUCE THE CONTENT OF AN AGRICULTURAL PRODUCT IN AFLATOXINS |
| CN102239959A (en) * | 2010-05-11 | 2011-11-16 | 姜明一 | Preparation method of ion activation liquid |
| CN102687800A (en) * | 2012-05-03 | 2012-09-26 | 山东农业大学 | Preparation method of aflatoxin B1 biological degradation agent and application thereof |
| CN113318701A (en) * | 2021-05-20 | 2021-08-31 | 河源市食品检验所 | Removing aflatoxin B in soil-pressed peanut oil1Adsorbent and preparation method thereof |
-
2022
- 2022-02-21 CN CN202210157821.0A patent/CN114504072A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR7401040A (en) * | 1973-02-13 | 1975-11-04 | Battelle Memorial Institute | PROCESS TO REDUCE THE CONTENT OF AN AGRICULTURAL PRODUCT IN AFLATOXINS |
| CN102239959A (en) * | 2010-05-11 | 2011-11-16 | 姜明一 | Preparation method of ion activation liquid |
| CN102687800A (en) * | 2012-05-03 | 2012-09-26 | 山东农业大学 | Preparation method of aflatoxin B1 biological degradation agent and application thereof |
| CN113318701A (en) * | 2021-05-20 | 2021-08-31 | 河源市食品检验所 | Removing aflatoxin B in soil-pressed peanut oil1Adsorbent and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 姜天童等: "《猪病防治新技术》", vol. 1, 湖北科学技术出版社, pages: 244 * |
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Application publication date: 20220517 |