CN114503999B

CN114503999B - Application of Cockerobacter roseum SDB9 and Brevibacterium frigidum SDB5 in regulation and control of potatoes

Info

Publication number: CN114503999B
Application number: CN202210148562.5A
Authority: CN
Inventors: 王建武; 相微微; 王宏梅
Original assignee: Yulin Zhongtai Agricultural Technology Co ltd
Current assignee: Yulin Zhongtai Agricultural Technology Co ltd
Priority date: 2022-02-17
Filing date: 2022-02-17
Publication date: 2022-11-15
Anticipated expiration: 2042-02-17
Also published as: CN114503999A

Abstract

The invention relates to application of Cockerella rosea SDB9 and Brevibacterium frigostolerant SDB5 in regulation and control of potatoes, and belongs to the technical field of microbial application. The Cocker roseum SDB9 and the Brevibacterium frigidum SDB5 are used independently or in combination, have different influences on the development and the nutritional quality of potato tubers, can regulate and control the development of potato tubers, improve the nutritional quality of potatoes and improve the commodity potato rate, and the number of the tubers of each plant is changed along with different use modes. Thereby laying a foundation for regulating and controlling the development and the nutritional quality of the potato tubers by using a microbiological method.

Description

Application of Cockerobacter roseum SDB9 and Brevibacterium frigidum SDB5 in regulation and control of potatoes

Technical Field

The invention relates to the technical field of microorganism application, in particular to application of Cockerella rosea SDB9 and Brevibacterium frigostolerant SDB5 in regulation and control of potatoes.

Background

Plant Growth-Promoting Rhizobacteria (PGPR) is a general term for useful Rhizobacteria that live in the rhizosphere micro-area of plants and antagonize pathogenic bacteria or promote Plant Growth. The growth-promoting rhizobacteria can promote the absorption of plants to nutrients and improve the resistance of a host system by secreting hormones such as indoleacetic acid, cytokinin and the like so as to improve the growth condition of the plants. In earlier researches, 2 PGPR bacteria, namely Cockerella rosea SDB9 and Brevibacterium frigidum SDB5 are separated from the rhizosphere of sabina vulgaris, and both the two bacteria are salt-resistant and alkali-resistant, can synthesize auxin indoleacetic acid and cytokinin at high level, are natural plant growth regulators, can remarkably promote the growth of plants such as rice, corn, mung bean and the like, and improve the salt-resistant property and the yield of the plants. According to the invention, on the basis of the previous period, the application effects of the two strains on the potatoes are analyzed, and the two strains are found to have the capacity of regulating and controlling the tuber development and the nutritional quality of the potatoes.

Potatoes are the fourth largest food crop next to rice, wheat and corn in the world, and are widely used, and in general, the higher the starch content and the vitamin C content of the potatoes, the better the nitrate content, the lower the nitrate content, the potato with low reducing sugar content is suitable for frying, and the potato tubers processed into potato chips are not suitable for being too large or too small. Therefore, in life and industrial production, the potato tuber has various requirements on the size and the nutritional quality of the potato tuber, and has important application value in regulating the growth and the nutritional quality of the potato tuber.

In the long-term evolution process, microorganisms from natural sources and plants can form a mutual-benefit symbiotic relationship, beneficial plant growth-promoting rhizobacteria are non-toxic and harmless, and the fermentation cost is low. Therefore, the method for regulating and controlling the development and the nutritional quality of potato tubers by utilizing the microbial agent, namely the agricultural green input product, is an environment-friendly and sustainable method, and is suitable for large-scale popularization and application.

Disclosure of Invention

The invention aims to solve the technical problems of regulation and control of development and nutritional quality of potato tubers by using plant rhizosphere growth-promoting bacteria, and solves the problems of environmental unfriendliness, high cost, high technical difficulty and the like in the prior art.

In order to solve the technical problems, the invention is realized by the following technical scheme:

the preservation number of the Kocuria rosea (Kocuria rosea) SDB9 is CGMCC No.17922; the preservation number of Brevibacterium frigoritolerans (SDB 5) is CGMCC No.20136.

The application of Cockera rosea SDB9 and Brevibacterium frigostolerant SDB5 in regulating and controlling development and nutritional quality of potato tubers comprises the following specific steps:

from 1X 10 ⁶ -1×10 ¹⁰ Selecting the use concentration of the bacterial liquid within the range of CFU/mL, preferably, the use concentration of the bacterial liquid of the Coccocus roseus SDB9 and Brevibacterium hardtii SDB5 is 1X 10 ⁷ CFU/mL, and the two bacteria are mixed in equal proportion when used in combination. After the potato tubers are cut, the wound surface is large, a large number of nutrient substances easily flow out, and the Cockerta roseum SDB9 and the Brevibacterium frigidum SDB5 are easily planted on the tubers, so that the inoculation mode of the potato tubers is simple and easy. By dilutingWashing tubers with bacterial liquid; or packaging tubers with mesh bags, and directly soaking in the bacterial solution; or filling tubers in a basket, and directly leaching with the bacterial liquid; or the bacteria liquid is sprayed on the cut tubers by a sprayer, and the bacteria inoculation work can be finished. And drying the inoculated tubers in the shade for later use. Then sowing and managing are carried out according to a conventional mode.

The Cockerella rosea SDB9 and the Brevibacterium frigidum SDB5 have the capacity of regulating and controlling the development of potato tubers, improving the nutritional quality of potatoes and increasing the commodity potato rate.

The Cockerella rosea SDB9 is independently used, so that the yield of the potatoes is remarkably increased, the sizes of tubers are uniform, and the number of small potatoes is reduced;

when the brevibacterium frigidum SDB5 is singly used, the yield of potatoes is obviously increased, the number of tubers of a single plant is increased, and the number of large potatoes and small potatoes is increased;

by using the Cockerella rosea SDB9 and the Brevibacterium hardtii SDB5 in a combined manner, the yield of the potatoes is obviously increased, the number of tubers of a single plant is reduced, the volume and the mass of large potatoes are increased, and the size difference between the large potatoes and small potatoes is increased.

When the Coccocus roseus SDB9 is independently used, the content of vitamin C, soluble sugar and reducing sugar in the potatoes is improved, and the content of nitrate is reduced;

when the brevibacterium frigidum SDB5 is singly used, the content of vitamin C and soluble sugar in the potatoes is increased, and the content of reducing sugar and nitrate is reduced;

by using the Cockerella rosea SDB9 and Brevibacterium frigidum SDB5 in combination, the content of soluble sugar in the potato is increased, and the content of vitamin C, reducing sugar and nitrate is reduced.

The invention has the beneficial effects that:

the invention utilizes plant rhizosphere growth-promoting bacteria, namely Cockerella rosea SDB9 and Brevibacterium frigostolerant SDB5 to regulate and control the development and the nutritional quality of potato tubers. The Cocker roseum SDB9 and the Brevibacterium frigidum SDB5 are used independently or in combination, have different influences on the development and the nutritional quality of potato tubers, can regulate and control the development of potato tubers, improve the nutritional quality of potatoes and improve the commodity potato rate, and the number of the tubers of each plant is changed along with different use modes. The application method has the advantages of low technical difficulty, environmental friendliness, low cost, simplicity, convenience and feasibility.

Drawings

FIG. 1 is a graph showing the results of measuring the vitamin C content of Kexin No. one potato;

FIG. 2 is a graph showing the results of measuring the nitrate content of Kexin No. potato;

FIG. 3 is a graph showing the results of starch content measurement of gram New first potato;

FIG. 4 is a graph showing the results of measuring the soluble sugar content of each gram of New first potato;

FIG. 5 is a graph showing the results of measuring the reducing sugar content of each gram of New first potato;

FIG. 6 is a graph showing the stem weight measurement results of individual pieces of the green potato No. 9 potato;

FIG. 7 is a graph showing the stem number measurement results of individual pieces of a potato No. 9;

FIG. 8 is a graph showing the results of determination of the potato commodity rate of a potato No. 9;

FIG. 9 is a graph showing the results of measurement of potato yield per mu of potato No. 9.

Detailed Description

The present invention will be described in further detail below: the present embodiment is implemented on the premise of the technical solution of the present invention, and a detailed implementation is given, but the scope of the present invention is not limited to the following embodiments.

Example 1: area test

(1) Potato test varieties: kexin No. I (purple flower white) is one of the local dominant varieties of Yulin.

(2) Treatment of test potato material:

selecting healthy potato tubers with substantially consistent color and size and no plant diseases and insect pests, dividing into 4 groups, and respectively using the tubers with concentration of 1 × 10 ⁶ -10 ⁷ Soaking and washing the CFU/mL SDB5 bacterial liquid, the SDB9 bacterial liquid, the SDB5+ SDB9 mixed bacterial liquid in equal proportion and equal amount of clear water (contrast), and drying in the shade for later use. The test adopts a single-factor random block design, 3 repetitions are treated in 4 ways, and the area of each repetition cell is 40m ² Is long and long8m, 5m wide, 1 hole/hole seeding rate, 70cm row spacing, 50cm plant spacing, and 4 protective rows arranged around. And managing according to the traditional planting mode.

(3) Statistical results of plant height:

the plant height is measured from the root-stem junction to the highest point, and 10 plants are randomly selected for statistics. The results show that: the height variation of each treated plant is 70.27-79.87cm, and each treatment is higher than that of the control. Wherein the plant height of SDB9 is 79.87cm, which is 13.66% higher than that of the control treatment; the second digit was SDB5+ SDB9 at 78.53cm, 11.76% higher than control; the third bit was SDB5 at 74.8cm, 6.45% higher than the control treatment (Table 1).

TABLE 1 g New Potato height

Treatment of	I	II	III	Average (cm)	Contrast increase and decrease (%)
						SDB5	79.20	80.80	64.40	74.80	6.45
SDB9	85.10	70.50	84.00	79.87	13.66
						SDB5+SDB9	73.00	84.50	78.10	78.53	11.76
Control (CK)	82.20	60.30	68.30	70.27	0.00

(4) Counting the weight of each individual tuber:

10 random plants were taken for statistics, and the results show that: the weight variation of each single tuber is 1.12-1.56kg, and each treatment is higher than the control. Wherein the tubers of the SDB5 and the SDB9 have the highest weight, which is 1.29kg and is 14.88 percent higher than that of the control treatment; the second bit was SDB5, 1.25kg, 11.31% higher than control treatment (table 2).

TABLE 2g weight of individual tubers of New first potato

Treatment of	I	II	III	Average (kg)	Contrast increase and decrease (%)
						SDB5	1.27	1.09	1.38	1.25	11.31
SDB9	1.47	1.18	1.21	1.29	14.88
						SDB5+SDB9	1.09	1.56	1.21	1.29	14.88
Control (CK)	1.14	0.95	1.28	1.12	0.00

(5) Counting the tubers of the single plants:

randomly taking 10 plants for statistics, and the result shows that: the number of tubers of each treated plant varied from 9.43 to 10.63, and the treatments were higher than the control except that the SDB5+ SDB9 treatments were lower than the control. Wherein, the number of single tubers of SDB5 is the highest and is 10.63, which is 19.96 percent higher than that of the control treatment; the second SDB9, 10.3, was 6.51% higher than the control; the SDB5+ SDB9 treatment was a minimum of 9.43, 2.45% lower than the control treatment (table 3).

TABLE 3 grams of the number of tubers of the New first potato plant

Treatment of	I	II	III	Average (one)	Contrast increase and decrease (%)
						SDB5	11.5	9.7	10.7	10.63	9.96
SDB9	12.6	9.2	9.1	10.30	6.51
						SDB5+SDB9	7.2	11.8	9.3	9.43	-2.45
Control (CK)	10.2	9	9.8	9.67	0.00

(6) And (4) counting the commodity potato rate:

each cell takes 10 square meters for statistics, the specification of the commercial potatoes is more than 150 grams, and the small potatoes are smaller than 150 grams, and the result shows that: the commercial potato rate variation of each treatment is 53.23-58.44%, and each treatment is higher than the control. Wherein the commercial potato rate of SDB5+ SDB9 is the highest, 58.44%, which is 9.79% higher than that of the control treatment; the second bit was SDB5, 58.23% higher than control treatment; the third bit was SDB9, 55.63% higher than the control treatment by 4.5% (table 4).

TABLE 4 commercial Potato yields of New first Potato

Treatment of	I	II	III	Average (%)	Contrast increase and decrease (%)
						SDB5	49.03	65.42	60.24	58.23	9.39
SDB9	54.39	54.96	57.53	55.63	4.50
						SDB5+SDB9	68.09	59.85	47.39	58.44	9.79
Control (CK)	41.36	52.22	66.12	53.23	0.00

(7) And (3) counting the yield per mu:

the yield per mu statistics comprises commodity potatoes and non-commodity potatoes, 10 square meters of yield is measured in each cell, and the yield per mu is converted. The results show that: the acre yield variation of each treatment is 2378.22-2753kg, and each treatment is higher than the control. Wherein, the yield per mu of SDB5 is the highest, 2753.10kg, which is 15.76 percent higher than that of the control treatment; the second bit was SDB5+ SDB9, 2680.26kg, 12.7% higher than control; the third bit was SDB9, 2597.54kg, 9.22% higher than control treatment (table 5).

TABLE 5 mu yield of New first potato

(8) Determination results of vitamin C content:

wherein, represents significant difference (P < 0.05), and represents significant difference (P < 0.01), as follows.

By adopting a fruit and vegetable vitamin C content determination method (2, 6-dichloroindophenol titration method) (GB/T6195-1986), as can be seen from figure 1, the inoculation of different microbial inoculum has certain influence on the vitamin C content of the potato. Except that the content of SDB9+5 vitamin C in the treatment group is lower than that of CK in the control group, the content of the SDB9+5 vitamin C in the other treatment groups is higher than that of the CK in the control group, wherein the content of the SDB9 vitamin C is the largest and reaches 30.74 mu g/kg, and is 77% higher than that of the CK, and the difference with the control group exists. The content of SDB5 vitamin C is 26.07 mug/kg, which is 51 percent higher than CK, and the difference with CK is obvious, the content of SDB9+5 vitamin C is 14.90 mug/kg, which is 14 percent lower than the control group, and the difference with CK is not obvious. Comprehensively considering, the vitamin C content of the potatoes can be improved by singly matching the two rhizosphere growth-promoting bacteria, the treatment effect of the SDB9 is most obvious, and the vitamin C content can be obviously reduced by mixing the two rhizosphere growth-promoting bacteria.

(9) Nitrate content determination results:

as shown in FIG. 2, the nitrate content of potato can be reduced to different degrees in all three treatment groups by using a method for measuring the nitrite and nitrate contents of fruits, vegetables and products thereof (GB/T15401-1994), wherein the nitrate content is remarkably reduced by SDB9 and SDB9+ 5. The nitrate content of CK in the control group is 96.28g/kg, the nitrate content of SDB5, SDB9 and SDB9+5 in the test groups is 85.24g/kg, 77.17g/kg and 72.92g/kg respectively, and compared with the control group, the nitrate content is respectively reduced by 11%, 20% and 24%, wherein SDB9 and SDB9+5 are obviously different from CK. Comprehensively considering, different rhizosphere growth-promoting bacteria can reduce the content of nitrate in the potatoes, and the SDB9+5 treatment effect is most obvious.

(10) And (3) starch content determination results:

as can be seen from FIG. 3, the starch content of potato was not significantly affected by the different treatments, as measured by the method for measuring starch in food (GB/T5009.9-2003). Numerically, the starch content in the four treatment groups varies from 59.27 to 70.24mg/g, and the starch content in the test groups is higher than that in CK. Wherein, the starch content of the SDB9+5 treatment group is the highest and is 70.24mg/g, which is improved by 16 percent compared with CK, and the starch content of the SDB5 and SDB9 treatment groups is respectively 64.79mg/g and 61.76mg/g, which is improved by 7 percent and 2 percent compared with CK. Overall, both rhizosphere growth promoting bacteria increased starch levels, with the SDB9+5 treatment being most pronounced but not significantly different from the control.

(11) Soluble sugar content determination results:

the method for measuring the soluble sugar of the fruits and the vegetables (GB/T6194-1986) is adopted for measurement, and as can be seen from figure 4, different treatments have different influences on the soluble sugar content of the potatoes. Numerically, in the four treatment groups, the soluble sugar content amplitude is 19.94-38.98mg/g, and the soluble sugar content in the test group is higher than that in CK. The content of soluble sugar in the SDB5 treatment group is the highest and is 38.98mg/g, the content is improved by 95% compared with CK, the content is SDB9+5 in the second place, the content is 38.48mg/g and is improved by 93% compared with CK, the content is SDB9 in the third place, the content is 24.04mg/g and is improved by 21% compared with CK. Overall, two species of rhizosphere-promoting bacteria can increase the soluble sugar content, with a very significant difference between SDB5 and SDB9+5 treatments versus CK and a significant difference between SDB9 treatments versus CK.

(12) And (3) the content determination result of reducing sugar:

as shown in FIG. 5, when the reducing sugar content in the food was measured by the method for measuring reducing sugar (GB/T5009.7-2003), the reducing sugar content in potatoes was affected differently by different treatments. From the value, the SDB9 has the highest reducing sugar content which is 0.08, is improved by 60 percent compared with a control group, and has extremely obvious difference with CK; the reducing sugar content of other treatments is lower than that of CK, wherein the reducing sugar content of CK is 0.05, the reducing sugar content of SDB5 is 0.04, the reducing sugar content of the CK is reduced by 20% compared with that of CK, the reducing sugar content of the CK is remarkably different from that of CK, the reducing sugar content of SDB9+5 is the lowest, the reducing sugar content of the SDB is 0.02, the reducing sugar content of the SDB is reduced by 60% compared with that of CK, and the reducing sugar content of the CK is remarkably different from that of CK. Overall, although the reducing sugar content is not high, both can affect potato reducing sugar content depending on the growth-promoting bacteria: the SDB9 greatly improves the content of reducing sugar, the SDB5 greatly reduces the content of reducing sugar, and the SDB9+5 greatly reduces the content of reducing sugar.

Example 2: production test

(1) Potato test varieties: the number 9 of sweet potato is one of the local major varieties of elm forest.

(2) Treatment of the potato material tested:

respectively with a concentration of 1X 10 ⁶ -10 ⁷ And soaking and washing the cut potato seeds with CFU/mL SDB5 bacterial solution, SDB9 bacterial solution, SDB5+ SDB9 mixed bacterial solution in equal proportion, and drying in the shade for later use. Each treatment was seeded 1 acre with a machine. And managing according to the traditional planting mode.

(3) And (3) observing the shape of the tuber:

a hole is randomly selected for each treatment, all tubers are dug out for observation, and the result shows that: the production test result is basically consistent with the previous year regional test result, when the Cockerella rosea SDB9 is used alone, the sizes of the potato tubers are uniform, and the small potatoes are few; when the brevibacterium frigidum SDB5 is used alone, the large potatoes and the small potatoes are more; when the two kinds of bacteria are used in combination, the large potatoes can grow bigger, and the size difference between the large potatoes and the small potatoes is increased.

(4) Counting the weight of each tuber:

the 10 plants are randomly selected for statistics, and as can be seen from FIG. 6, the weight variation of each single-plant tuber is 2.419-2.904kg, and each treatment is higher than the control. Wherein, the single plant tuber of SDB9 has the highest weight of 2.904kg, which is 35.7 percent higher than that of the control treatment; the second bit was SDB5, 2.829kg, 32.2% higher than control treatment; SDB9+5 treatment was 13% higher than control.

(5) Counting the tubers of the single plants:

the 10 plants were randomly selected for statistics, and as can be seen from FIG. 7, the number variation of tubers of each treated individual plant was 8.18-9.43, the SDB9 and SDB9+5 treatments were lower than the control by 11.1% and 5.4%, respectively, and the SDB5 treatments were all higher than the control. Among them, SDB5 has the highest number of tubers, 9.43, which is 2.5% higher than the control.

(6) And (4) counting the commodity potato rate:

each treatment is carried out by randomly taking 10 square meters for statistics, the statistics is repeated for 3 times, and the specification of the commercial potatoes is more than 150 grams, and the potatoes are small potatoes with the weight of less than 150 grams. As can be seen from FIG. 8, the potato rate of each treatment varied from 56.22 to 87.2%, and each treatment was higher than the control. Wherein the commercial potato yield of SDB9 is the highest and is 87.2 percent, which is 55.1 percent higher than that of the control treatment; the second bit was SDB9+5, 85.2% higher than control treatment; the third bit was SDB5, 83.5% higher than the control treatment.

(7) And (3) carrying out statistics on the yield per mu:

the yield per mu statistics comprises commodity potatoes and non-commodity potatoes, 10 square meters are randomly taken for each treatment, the yield is measured, the treatment is repeated for 3 times, and the yield per mu is converted. As can be seen from FIG. 9, the acre yield of each treatment is 3539.25-4553.59kg, and each treatment is higher than the control. Wherein, the acre yield of SDB5 is the highest, namely 4553.59kg, which is 49.6 percent higher than that of the control treatment; the second bit was SDB9, 4231.85kg, 38.9% higher than control treatment; the third bit was SDB9+5, 3539.25kg, 16.2% higher than control treatment.

The above description is only a preferred embodiment of the present invention, and these embodiments are based on different implementations of the present invention, and the scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are included in the scope of the present invention.

Claims

1. Rose color Corkspora bacteria (C.) (Kocuriarosea) SDB9 and Brevibacterium frigidum ((S))Brevibacteriumfrigoritolerans) SDB5 in the regulation of potatoes, characterized in that: cocker roseum (Kocuria ro)sea) the preservation number of the SDB9 is CGMCC No.17922; the preservation number of Brevibacterium frigoritolerans (SDB 5) is CGMCC No.20136;

the Cocker roseochracea SDB9 is independently used, so that the yield of potatoes is remarkably increased, the sizes of tubers are uniform, the number of small potatoes is reduced, the contents of vitamin C, soluble sugar and reducing sugar in the potatoes are all improved, and the content of nitrate is reduced;

when the brevibacterium frigidum SDB5 is singly used, the yield of potatoes is obviously increased, the number of tubers of a single plant is increased, large potatoes and small potatoes are increased, the contents of vitamin C and soluble sugar in the potatoes are increased, and the contents of reducing sugar and nitrate are reduced;

by using the Cockerella rosea SDB9 and the Brevibacterium frigidum SDB5 in a combined manner, the yield of the potatoes is obviously increased, the number of single tubers is reduced, the large potatoes are increased, the size difference between the large potatoes and the small potatoes is increased, the content of soluble sugar in the potatoes is increased, and the content of vitamin C, reducing sugar and nitrate is reduced.