Disclosure of Invention
The embodiment of the invention provides a protection circuit of a biological sample preparation device, which aims to solve the problem of short circuit between any metal terminals and between any electric loops of biological sample preparation device equipment with exposed metal terminals in the prior art.
In a first aspect, a biological sample preparation device is provided that includes a housing, a plurality of motors, a plurality of test receptacles, a plurality of heating apparatuses, and a plurality of protection circuits;
the protection circuit includes:
a control module;
the first loop comprises a constant current source and a resistance value detection module; and
the second loop comprises a power supply and an overcurrent detection module, and is connected to two ends of the power supply and is provided with a second load resistor;
the resistance value detection module detects the resistance value of a first load resistor at two ends of the constant current source and sends the resistance value to the control module; if the resistance value of the first load resistor is not in the preset range, the control module controls the power supply to not supply power; if the resistance value of the first load resistor is in a preset range and the overcurrent detection module detects overcurrent, the control module controls the power supply source to not supply power; if the resistance value of the first load resistor is in a preset range and the overcurrent detection module does not detect overcurrent, the control module controls the power supply source to supply power;
the motors are arranged in the shell, and driving shafts of the motors are respectively connected with the experiment containers to drive the experiment containers to rotate;
the two ends of the constant current source of each first loop form a first terminal which is exposed out of the shell;
two ends of a second load resistor of each second loop are used as two nodes to form a second terminal, and the second terminals are exposed out of the shell;
when each heating device clamps the experimental container for heating, the first load resistor is contacted with the first terminal to form a closed first loop, and the second load resistor is contacted with the second terminal to form a closed second loop.
According to the embodiment of the invention, the load resistance values at the two ends of the constant current source in the first loop are detected, if the load resistance values are not in the preset range, the second loop is not supplied with power, and if the load resistance values are in the preset range, whether the second loop is supplied with power is judged according to the overcurrent detection of the second loop, and the short circuit of the first loop excited to be the constant current source does not cause large current, so that the short circuit protection between any circuits is realized.
Detailed Description
Embodiments of the present invention are described in detail below, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to like or similar modules or modules having like or similar functions throughout. The embodiments described below by referring to the drawings are illustrative only and are not to be construed as limiting the invention. On the contrary, the embodiments of the invention include all alternatives, modifications and equivalents as may be included within the spirit and scope of the appended claims.
According to the embodiment of the invention, the load resistance values at the two ends of the constant current source in the first loop are detected, if the load resistance values are not in the preset range, the second loop is not supplied with power, and if the load resistance values are in the preset range, whether the second loop is supplied with power is judged according to the overcurrent detection of the second loop, and the short circuit of the first loop excited to be the constant current source does not cause large current, so that the short circuit protection between any circuits is realized.
Example 1
Fig. 1 is a schematic diagram of a protection circuit according to a first embodiment of the present invention. As shown in fig. 1, the protection circuit includes a first loop 1, a second loop 2, and a control module 3.
In the embodiment of the present invention, the first loop 1 includes a constant current source 11 and a resistance value detection module 12. The constant current source 11 is an excitation source of the first loop 1, and the voltage for supplying power to the circuit forming the constant current source 11 is low voltage which does not cause harm to human body, such as 3.3V. Connected to both ends of the constant current source 11 is a first load resistor R1, and the first load resistor R1 varies according to different loads actually connected to the first circuit 1. The resistance value detection module 12 detects the load resistance values of both ends of the constant current source 11, that is, the resistance value of the first load resistor R1, and sends it to the control module 3. In some embodiments, other parameters of the first loop 1 may also be detected and sent to the control module 3 for subsequent short-circuit protection control, which is not limited herein.
The second circuit 2 comprises a power supply 21, an overcurrent detection module 22 and a switching module 23. The power supply is an excitation source of the second circuit 2, and may be a common constant voltage power source, such as a 12V dc voltage source, which is not limited herein. Generally, the second load resistor R2 is connected to both ends of the power supply 21, and the second load resistor R2 varies according to the load actually connected to the second circuit 2. In the embodiment of the present invention, the switch module 23 is connected between the power supply end of the power supply 21 and the second load resistor R2. Preferably, the switching module 23 is composed of field effect transistors. The overcurrent detection module 22 is connected between the second load resistor R2 and the ground, and when only the second loop 2 is short-circuited, the short-circuit protection of the second loop 2 is realized.
The control module 3 adjusts the on or off of the switching module 23 according to the received resistance value of the first load resistor R1 to control whether the power supply 21 supplies power to the second load resistor R2. If the resistance value of the first load resistor R1 is not within the preset range, the control module 3 turns off the switch module 23 to control the power supply 21 not to supply power. If the resistance value of the first load resistor R1 is within the preset range and the overcurrent detection module 22 detects the overcurrent, the control module 3 turns off the switch module 23 to control the power supply 21 not to supply power. If the resistance value of the first load resistor R1 is within the preset range and the overcurrent is not detected by the overcurrent detection module 22, the control module 3 closes the switch module 23 to control the power supply 21 to supply power. Preferably, the control module 3 outputs a PWM (Pulse width modulation ) signal to the switch module 23 according to the first load resistor R1, the switch module 23 is closed, and the power supply 21 supplies power; the control module 3 does not output a PWM signal to the switching module 23 according to the first load resistor R1, the switching module 23 is turned off, and the power supply 21 does not supply power. The manner in which the switch module 23 is controlled to be turned on or off by the control module 3 is also referred to as a software control manner.
As an embodiment of the present invention, as shown in fig. 1, the overcurrent detecting module 22 is connected to the control module 3. When the overcurrent detection module 22 outputs the preset level, it indicates that the overcurrent detection module 22 detects the overcurrent of the second loop 2, and the control module 3 does not output the PWM signal to the switching module 23 to turn off the switching module 23. The software control mode can stably disconnect the switch module 23, so that the safety risk and false alarm caused by instant disconnection are avoided.
As another embodiment of the present invention, as shown in fig. 2, fig. 2 is a schematic diagram of another protection circuit according to a first embodiment of the present invention. The overcurrent detection module 22 is directly connected to the switch module 23. After detecting that the second loop 2 is over-current, the over-current detection module 22 outputs a preset level to disconnect the switch module 23, and controls the power supply 21 to not supply power. The manner in which the overcurrent detection module 22 is directly connected to the switch module 23 to control the on/off of the same is also referred to as a hardware control manner. For the switch module 23 composed of field effect transistors, the hardware control mode can rapidly disconnect the switch module 23, so that the safety risk caused by time delay disconnection is avoided.
By combining the characteristics of the software control mode and the hardware control mode, as shown in fig. 2, the embodiment of the invention adopts the software control mode and the hardware control mode at the same time, so that the protection circuit has a double overcurrent protection mechanism, and is safer, more stable and more reliable.
Fig. 3 is a schematic diagram of an overcurrent detection module according to an embodiment of the invention. As shown in fig. 3, the overcurrent detection module 22 includes a comparator 221 and a sampling resistor 222. A sampling resistor 222 is connected between the second load resistor R2 and ground. An input end of the comparator 221 is connected with the sampling resistor 222, the voltage of the sampling resistor 222 relative to the ground is compared, and an output end of the comparator 221 is respectively connected with the control module 3 and the switch module 23. When the second loop 2 is over-current, the comparator 221 detects that the voltage of the sampling resistor 222 relative to the ground is over-high, and the output terminal thereof outputs a low level (preset level) to the control module 3 and the switch module 23.
In some specific scenarios, the two ends of the first load resistor R1 and the two ends of the second load resistor R2 are exposed as terminals, and the user accesses different conductors according to actual needs, so that the protection circuit automatically judges whether to start short-circuit protection. The operation principle of the protection circuit of the embodiment of the invention is as follows.
In the first loop 1, when two nodes corresponding to the first load resistor R1 are shorted, the resistance value of the first load resistor R1 detected by the resistance value detection module 12 is 0, the resistance value of the first load resistor R1 is set to be not within a preset range, and the control module 3 turns off the switch module 23 to control the power supply 21 not to supply power. Thereby, the second circuit 2 is disconnected; the first circuit 1 is still closed, but since it is a constant current source, no large current is generated, and thus a short circuit in this case does not cause damage to the first circuit 1.
In the second loop 2, when two nodes corresponding to the second load resistor R2 are short-circuited, the resistance value detection module 12 detects that the first load resistor R1 is not in the preset range, and then determines the above situation; the resistance value detection module 12 detects that the first load resistor R1 is in a preset range and the overcurrent detection module 22 does not detect overcurrent, and the control module 3 enables the switch module 23 to be closed to control the power supply 21 to supply power; the resistance value detection module 12 detects that the first load resistor R1 is in a preset range and the overcurrent detection module 22 detects overcurrent, the overcurrent detection module 22 rapidly outputs a preset level to the switch module 23 to enable the switch module 23 to be disconnected, meanwhile, the overcurrent detection module 22 also outputs the preset level to the control module 3, the control module 3 does not generate a PWM signal to the switch module 23 to enable the switch module 23 to be disconnected after receiving the PWM signal, and the power supply 21 does not supply power. Thereby, the second circuit 2 is disconnected.
For the first loop 1 and the second loop 2, when any node at two ends of the second load resistor R2 is short-circuited with any node at two ends of the first load resistor R1, a resistor is additionally connected in parallel at two ends of the first load resistor R1, so that the load resistors at two ends of the constant current source are not only the first load resistor R1, the actual load resistance values at two ends of the constant current source at this time are not in a preset range, and the control module 3 enables the switch module 23 to be disconnected and controls the power supply 21 not to supply power. Thereby, the second circuit 2 is disconnected; and the power supply 21 does not form a new loop with the components in the first loop 1 to cause damage.
For the first loop 1 and the second loop 2, when four nodes are short-circuited at both ends of the first load resistor R1 and both ends of the second load resistor R2, the short-circuit protection can be performed similarly, which corresponds to the case where two nodes corresponding to the first load resistor R1 are short-circuited.
Further, when the two ends of the first load resistor R1 are not connected to the conductors, the resistance value of the first load resistor R1 detected by the resistance value detection module 12 is infinity, the infinity is set to be not in the preset range, and the control module 3 turns off the switch module 23 to control the power supply 21 not to supply power. Thereby, both the first circuit 1 and the second circuit 2 are disconnected. For the exposed four nodes, since the first loop 1 is low voltage which does not cause harm to human body, the switch module 23 in the second loop 2 is disconnected to disconnect the power supply 21 from the second load resistor R2, so that the user touches the four nodes, and no safety risk exists.
In some embodiments, the first loop 1 further includes a resistive temperature sensor 13, where the resistive temperature sensor 13 is connected in parallel to two ends of the constant current source 11 as a first load resistor R1, and is connected into the first loop 1. The resistance value exhibited by the resistive temperature sensor 13 itself varies with the temperature it senses. The second circuit 2 further comprises a heating film which is fed into the second circuit 2 as a second load resistor R2. According to the scene, the preset range is reasonably set, namely whether the resistance temperature sensor 13 and the heating film are in a normal working state or not can be judged through the resistance value detection module 2, and whether the first loop 1 and the second loop 2 where the resistance temperature sensor and the heating film are located are short-circuited or not can be judged. Judging whether the load resistance values at the two ends of the constant current source 11 are in a preset range or not can also be converted into judging whether the temperature sensed by the resistance type temperature sensor 3 is in the preset range or not, and then judging whether the resistance type temperature sensor 13 and the heating film are in a normal working state or not.
According to the embodiment of the invention, the load resistance values at the two ends of the constant current source in the first loop are detected, if the load resistance values are not in the preset range, the second loop is not supplied with power, and if the load resistance values are in the preset range, whether the second loop is supplied with power is judged according to the overcurrent detection of the second loop, and the short circuit of the first loop excited to be the constant current source does not cause large current, so that the short circuit protection between any circuits is realized.
Example two
In many experiments in biology, it is often necessary to simulate the body temperature of an organism or to maintain a specific temperature during the preparation of a biological sample in order to facilitate the performance of the biological experiment. But also requires that the individual experimental containers be heated while not affecting other operations of the biological assay.
Such as in the preparation of single cell suspensions, the operations of cutting, milling tissue, etc., are performed in the laboratory vessels while the biological sample in each of the laboratory vessels is maintained at a different specific temperature by a heating device. In this process, the maintained temperature cannot fluctuate excessively, otherwise, the biological sample may be denatured, and the result of the biological experiment may be affected. Therefore, the heating device can heat, and the temperature of the experimental container needs to be sensed in real time and fed back to a controller in the device to adjust the heating power so as to realize stable heating. After the single cell suspension is prepared, the heating device and the experimental container are required to be detached, and the single cell suspension in the experimental container is poured out. The next time single cell suspension preparation begins, the heating apparatus and the laboratory vessel are reloaded onto the apparatus. Therefore, the heating device needs to adapt to the usage scenario of repeated plugging and unplugging.
Fig. 4 is a schematic view of a biological sample preparation device according to a second embodiment of the present invention. As shown in fig. 4, the biological sample preparation device includes a housing, a plurality of motors, a plurality of test receptacles, a plurality of heating apparatuses, and a plurality of protection circuits (not shown) as described in connection with the first embodiment. In the embodiment of the present invention, the structure of the protection circuit is the same as that of the first embodiment, including all the features described in the first embodiment, and will not be described again here.
In the embodiment of the invention, a plurality of motors are arranged in the shell, and driving shafts of the motors are respectively connected with a plurality of experimental containers for preparing single-cell suspension to drive the experimental containers to rotate. The plurality of protection circuits are arranged in the shell, and each protection circuit corresponds to one experiment container and one heating device. In the protection circuit, two ends of a constant current source of the first loop 1 are exposed out of the shell and are connected with a resistance type temperature sensor in the heating equipment; two ends of a second load resistor R2 of the second loop 2 are exposed to the shell as two nodes for connecting a heating film in the heating equipment. In order to make the structure of the heating device compact, the first terminals are formed at both ends of the constant current source of the first circuit 1 to be exposed to the housing, and the second terminals are formed at both ends of the second load resistor R2 of the second circuit 2 as two nodes to be exposed to the housing. The first terminal and the second terminal are two-section pin terminals. The heating device only needs to reserve two interfaces to be connected with the first terminal and the second terminal. When each heating device clamps the experimental container for heating, the resistance type temperature sensor is contacted with the first terminal to form a closed first loop 1, and the heating film is contacted with the second terminal to form a closed second loop 2.
In an embodiment of the invention, the biological sample preparation device further comprises a microcontroller. The microcontroller controls the heating films respectively according to the temperatures measured by the resistance temperature sensors, so that biological samples in the experimental containers can be stably maintained at the same or different specific temperatures. The control module 3 in the protection circuit multiplexes the microcontroller of the biological sample preparation device to realize short-circuit protection.
According to the embodiment of the invention, the load resistance values at the two ends of the constant current source in the first loop are detected, if the load resistance values are not in the preset range, the second loop is not supplied with power, and if the load resistance values are in the preset range, whether the second loop is supplied with power is judged according to the overcurrent detection of the second loop, and the short circuit of the first loop excited to be the constant current source does not cause large current, so that the short circuit protection between any circuits is realized.
While embodiments of the present invention have been shown and described above, it will be understood that the above embodiments are illustrative and not to be construed as limiting the invention, and that variations, modifications, alternatives and variations may be made to the above embodiments by one of ordinary skill in the art within the scope of the invention.