CN114480739A - Method for preparing human papilloma virus capture probe set and application thereof - Google Patents
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Abstract
The invention discloses a method for preparing a high-risk human papilloma virus capture probe set and application thereof, belonging to the fields of gene detection and molecular genetics; the invention uses 15 high-risk plasmids HPV16, 18, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66 and 68 as templates, and amplifies the amplification product covering the whole length of HPV by using a rolling circle amplification method; further breaking the amplification product into different required lengths by ultrasound, namely obtaining a probe capable of specifically capturing 15 HPV genomes; the invention can reduce the preparation cost of the HPV capture probe group and shorten the preparation period of the probe group. Meanwhile, the probe set can accurately detect the infection and integration state of the high-risk HPV.
Description
The technical field is as follows:
the invention belongs to the field of gene detection and molecular genetics, and particularly relates to a preparation method and application of a 15 high-risk human papilloma virus genome capture probe.
Background art:
cervical cancer refers to a malignant tumor that occurs in the cervix, vagina and cervix, and is one of the most common gynecological malignancies, second only to breast cancer. Human Papilloma Virus (HPV) is a circular double-stranded small-molecule DNA virus without envelope coating, and the genome is about 8000 base pairs (bp). Numerous studies have shown that persistent infection with high-risk HPV types (HPV16, 18, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66 and 68) is the major cause of cervical intraepithelial neoplasia and cervical cancer. Nevertheless, not every patient infected with high-risk HPV develops cervical cancer. With the progress of molecular biology and cytogenetics, scientific research finds that the occurrence of HPV DNA and cervical epithelial cell genome integration events is an important link in the cervical cancer generation process. Once inserted into the genome of cervical epithelial cells, HPV DNA sequences will replicate as the human genome replicates, resulting in the sustained expression of oncoproteins. Meanwhile, the genome structure of the cervical epithelial cells and the expression level of related genes can be changed, so that the instability of the genome of the epithelial cells is increased, the cervical epithelial cells are abnormal in function and uncontrolled in division, and finally, cancer is generated. Currently, HPV genomic integration events are a well-established key marker for malignant transformation of cervical intraepithelial neoplasia into cervical cancer. In benign lesions caused by HPV, the viral DNA inside the cell is usually located outside the cervical epithelial genome in free form. In high-grade lesions and cervical cancer, HPV DNA is usually inserted into the genome of epithelial cells in an integrated form, and the integration rate increases significantly with the severity of cervical lesions. Therefore, the integration of HPV and human genome can be used as an auxiliary diagnostic tool for cervical high-grade lesion and cervical cancer. High throughput sequencing technology combined with targeted capture technology is currently the most accurate method for detecting the HPV integration state. One of the core materials for HPV targeted capture is HPV DNA capture probe, and currently, the major DNA capture probe manufacturers on the market have Roche (Roche), IDT (integrated DNAtechnologies), Twist Bioscience, and currently, three companies adopt a scheme for directly synthesizing oligo. However, the cost of the direct synthesis of the probe is high, which causes the market price of the DNA probe to be high and the synthesis period to be long.
The invention content is as follows:
in view of the above problems, the present invention aims to provide a preparation method and an application of a genome capture probe set for high-risk human papilloma virus. The preparation method is simple, high in yield and low in cost, and can shorten the preparation period to 1 day.
In order to achieve the purpose, the invention adopts the following technical scheme:
a method for preparing a DNA capture probe for capturing and sequencing high-risk HPV (human papillomavirus) genomes sequentially comprises the following steps:
(1) HPV16(SEQ ID NO.1), 18(SEQ ID NO.2), 31(SEQ ID NO.3), 33(SEQ ID NO.4), 35(SEQ ID NO.5), 39(SEQ ID NO.6), 45(SEQ ID NO.7), 51(SEQ ID NO.8), 52(SEQ ID NO.9), 53(SEQ ID NO.10), 56(SEQ ID NO.11), 58(SEQ ID NO.12), 59(SEQ ID NO.13), 66(SEQ ID NO.14) and 68(SEQ ID NO.15) were synthesized as high-risk types of HPV, and cloned into a pUC57 vector;
(2) synthesizing an amplification primer, wherein the nucleotide sequence of the primer is SEQ ID NO. 16;
(3) performing rolling circle type amplification by using dNTP of a modified label as a raw material and the plasmids in the step (1) as templates and the primer in the step (2);
(4) quantifying and mixing the obtained amplification products;
(5) purifying the mixed product obtained in the step (4) and breaking the product by an ultrasonic breaking instrument;
rolling circle amplification is a high-efficiency amplification system with amplification efficiency higher than that of PCR, and 1-10 mug of product can be obtained by 1ng of DNA generally; and rarely introduce mutation in the amplification process; the problem of the accumulative error rate faced by the current probe synthesis is avoided, so that the uniformity of the obtained probe is very high.
In addition, in the process of synthesizing DNA probes, synthesis is limited by technology, so that long-fragment probes are difficult to synthesize, and the length of the synthesized probes is generally not more than 150nt at present. The primer sequences at both ends of the amplification probe are removed, and the effective probe length is generally 120 nt. The HPV DNA probe prepared by the method of the invention has the length of tens of kb, and can be broken to any required length by an ultrasonic breaking method.
As a preferred embodiment of the present invention, the dNTPs in step (3) include dATP, dGTP, dCTP, dTTP and dUTP, wherein only dUTP is labeled with biotin-dUTP.
More preferably, the dTTP: the molar ratio of biotin-dUTP is 4: 1; the (biotin-dUTP + dTTP): dATP: dGTP: the molar ratio of dCTP used was 1:1:1: 1.
In a preferred embodiment of the present invention, when preparing HPV DNA probes, the DNA polymerase used in the rolling circle amplification in step (3) is Phi29DNA polymerase.
As a preferred embodiment of the present invention, the ultrasound in step (4) is to break the amplification product to 200-400 nt.
Further, the parameters of the ultrasound are set as: 60 cycles were interrupted (20 seconds of sonication, 30 seconds of rest).
The second purpose of the invention is to disclose 15 high-risk HPV capture probes prepared by the method.
The third purpose of the invention is to disclose the application of the probe in the target capture sequencing.
Further, the invention provides a method for targeted HPV genome sequencing of the HPV probes prepared by the method, which comprises the following steps:
(1) sample DNA extraction, fragmentation and construction of DNA library
(2) Library target region targeted capture and quantitation
(3) High throughput sequencing
(4) Bioinformatic analysis of gene sequences
The invention has the advantages that:
(1) the method can be used for producing the HPV DNA capture probe, has simple preparation method, large yield and low cost, and can shorten the preparation period to 1 day.
(2) In addition, the length of the probe prepared by the method is not limited, and probes with different targets and different lengths can be provided according to needs so as to meet the requirements of different probes.
Drawings
FIG. 1 shows the results of gel electrophoresis of the products of rolling circle amplification of example 1 of the present invention.
Detailed Description
To better illustrate the objects, aspects and advantages of the present invention, the present invention will be further described with reference to the accompanying drawings and specific embodiments.
Example 1
The method for preparing the high-risk HPV DNA probe comprises the following steps:
1. constructing pUC57 plasmid containing 15 high-risk HPV DNA sequences:
(1) high-risk HPV sequences (reference sequences from NCBI database) were synthesized by the biotechnology limited jinsler, south kyo.
(2) Respectively cloning the synthesized HPV DNA sequences to a pUC57 vector to obtain 15 high-risk HPV plasmids.
2. Rolling circle amplification and purification
(1) Preparing a reaction system: 2uL Phi29DNA polymerase buffer (10X concentration, purchased from Thermo fisher), 7.5uL amplification primer (100. mu.M concentration), 3uL HPV plasmid (10 ng/uL concentration), water to 24.15 uL.
(2) Incubate at 95 ℃ for 3 min, and keep on ice for 5 min
(3) Configuring a reaction system (related reagents are purchased from Thermo fisher): 0.75uL of dATP (10 mM concentration), 0.75uL of dGTP (10 mM concentration), 0.75uL of dCTP (10 mM concentration), 0.6uL of dTTP (10 mM concentration), 1.5uL of biotin-dutp (1 mM concentration) and 1.5uL of Phi29DNA polymerase (10U/. mu.L). And transferred to the above reaction system to obtain a mixture in a total volume of 30. mu.L.
(4) Placing the mixed reaction solution in a PCR instrument, wherein the reaction conditions are as follows: 30 ℃ C (incubation for 16 hours) and 65 ℃ C (inactivation for 10 minutes) to obtain an amplification product.
(5) A portion of the product was subjected to gel electrophoresis, and the result is shown in FIG. 1 (gel electrophoresis result of the product of rolling circle amplification).
(6) The amplification product was purified using purified magnetic beads purchased from Novovovomedium.
(7) The concentrations of the purified 15 high-risk HPV amplification products were measured with a QubitTM dsDNA Assay Kit (Thermo Fisher) and diluted to 200ng/uL with non-enzymatic water according to the measured concentrations.
(8) And mixing the diluted 15 high-risk HPV amplification products in equal proportion to obtain an initial full-length probe.
3. Probe ultrasound interruption
(1) Probe disruption was performed using a Biorupter ultrasonic disruptor with parameters set to: starting for 20s, stopping for 30s, and 60 cycles
(2) The probe concentration was measured by the QubitTM ssDNA Assay Kit (Thermo Fisher, cat # Q10212) reagent and the probe length was measured by agarose gel electrophoresis, with the cleaved probe band being 200-300 nt.
Example 2 method for high risk HPV DNA targeting capture by the probes of the invention
1. Pre-library construction
(1) DNA enzyme digestion fragmentation, end repair and A adding treatment
(a) The following reaction systems were prepared in PCR tubes on ice: 500ng of genome DNA, 10uL of enzyme mixture, and 60uL of enzyme-free water
(b) The PCR tube was placed in the PCR machine, hot capped at 105 ℃ and the following procedure was run: 4 deg.C (1 min), 30 deg.C (12 min), 72 deg.C (20 min), 4 deg.C (hold).
(2) Joint connection
(a) Thawing the adaptor connection buffer solution, the adaptor ligase and the DNA adaptor, reversing, uniformly mixing, and placing on ice for later use;
(b) the following reaction systems were prepared in PCR tubes on ice: 60. mu.L of the product of the previous step, 5. mu.L of linker ligase, 30. mu.L of linker ligation buffer and 5. mu.L of DNA linker.
(c) The mixture was shaken and mixed well and centrifuged briefly to collect the reaction solution to the bottom of the tube.
(d) Place the PCR tube in the PCR instrument (hot lid closed) and run the following program: 4 deg.C (1 min), 20 deg.C (15 min), 4 deg.C (hold).
(3) Linker ligation product purification
(a) Adding 60 mu L of purified magnetic beads into 100 mu L of the joint connection product, uniformly mixing, and incubating for 5 minutes at room temperature;
(b) place the PCR tube in a magnetic rack and after the solution is clear (about 5 minutes), carefully remove the supernatant;
(c) keeping the PCR tube in the magnetic frame all the time, adding 200. mu.L of freshly prepared 80% ethanol to rinse the magnetic beads, incubating at room temperature for 30 seconds, carefully removing the supernatant, and repeating the step for 1 time;
(d) keeping the PCR tube in a magnetic frame all the time, uncovering and drying the magnetic beads for 5-10 minutes until no ethanol remains;
(e) taking out the PCR tube from the magnetic frame, adding 23 mu L of enzyme-free water for elution, gently blowing and uniformly mixing by using a pipette, and standing for 2 minutes at room temperature;
(f) the PCR tube was placed in a magnetic stand and after the solution cleared (about 5 minutes), 20. mu.L of the supernatant was carefully removed to a new PCR tube.
(4) Library amplification
(a) Unfreezing the PCR amplification primer 1 and the PCR reaction mixed solution, inverting and uniformly mixing, and placing on ice for later use;
(b) the following reaction systems (50 μ L) were prepared in PCR tubes on ice: purified adaptor ligation product (20. mu.L), PCR amplification primer 1 (5. mu.L), PCR reaction mix (25. mu.L);
(c) oscillating and mixing uniformly, and centrifuging for a short time to collect reaction liquid to the bottom of the tube;
(d) the PCR tube was placed in a PCR apparatus, hot covered at 105 ℃ and the following reactions were carried out (PCR reaction conditions in Table 1):
TABLE 1
(5) Amplification product purification
(a) Adding 45 mu L of purified magnetic beads into 50 mu L of amplification products, uniformly mixing by oscillation, and incubating for 5 minutes at room temperature;
(b) placing the PCR tube in a magnetic frame to separate magnetic beads and liquid, and removing supernatant after the solution is clarified;
(c) adding 200 μ L of 80% ethanol to wash the magnetic beads, incubating at room temperature for 30 seconds, removing the supernatant, and repeating the step 1 time;
(d) keeping the PCR tube in the magnetic frame all the time, and opening a cover to dry the magnetic beads for 5-10 minutes until no ethanol remains;
(e) adding 45 mu L of enzyme-free water into the magnetic beads dried in the step 4, uniformly mixing by vortex oscillation, placing at room temperature for 2 minutes, placing in a magnetic frame, standing for 5 minutes, and carefully transferring 43 mu L of supernatant into a new centrifuge tube;
(f) determination of library concentration and fragment size after library construction
2. Hybrid Capture
(1) Hybridization of
(a) Preparing mixed solution 1: 750ng library, 5 μ L of blocking sequence 1, 2 μ L of blocking sequence 2.
(b) And (3) taking out the blocking reagent and the probe in the kit, standing, thawing, shaking, uniformly mixing, and preparing a mixed solution 2 according to 5 mu L of the blocking reagent and 2 mu L of the probe (operation on ice).
(c) Taking out the hybridization buffer solution in the kit, placing the hybridization buffer solution in room temperature for thawing, taking out 20 mu L of the hybridization buffer solution after thawing, placing the hybridization buffer solution in a constant temperature mixer at 65 ℃ for incubation for standby.
(d) Add 13. mu.L of hybridization buffer to mix 2, mix well, and centrifuge briefly.
(e) Using a temperature-controllable vacuum drier, setting the drying temperature at 45-50 ℃, opening a tube cover until the volume of the mixed solution 1 is concentrated to be less than 10 mu L, supplementing the mixed solution to 10 mu L with non-enzyme water, slightly sucking, uniformly mixing, centrifuging for a short time, and placing on ice for later use. The concentration time should not be too long to prevent evaporation.
(f) The concentrated mixture 2 was incubated in a PCR apparatus at 95 ℃ for 5 minutes and at 65 ℃ for 2 minutes (hot lid temperature 105 ℃) without removal after the incubation was complete.
(g) The mixture 1 with the hybridization buffer added thereto was incubated in a PCR apparatus at 65 ℃ for 2 minutes (hot lid temperature 105 ℃). (h) And (3) sucking 20 mu L of the mixed solution 1, adding into the mixed solution 2, fully and uniformly mixing, hybridizing for 16-24 h at 65 ℃, keeping the hybridization product in a PCR instrument, and entering a capturing step.
(2) Capture
(a) Taking out T1 magnetic beads in advance, balancing for 30 minutes at room temperature, and shaking and mixing uniformly;
(b) placing 50 mu L T1 magnetic beads in a new centrifuge tube, placing the centrifuge tube on a magnetic rack, standing for 1 minute until the liquid is clear, and carefully sucking away the liquid (not sucking the magnetic beads);
(c) taking the centrifuge tube off the magnetic frame, adding 200 μ L of capture buffer solution, blowing and mixing uniformly, placing the centrifuge tube on the magnetic frame, standing for 1 minute until the liquid is clear, and carefully sucking away the liquid (not sucking magnetic beads);
(d) repeating the step (3) twice, and totally three times;
(e) adding 200 mu L of capture buffer solution into the magnetic beads again to resuspend the magnetic beads, adding the magnetic beads into the hybridization products after resuspension, and placing the PCR tube on a rotary spinner to combine for 30 minutes at room temperature, wherein the rotating speed is not more than 10 revolutions per minute;
(f) taking out the washing buffer solution 1 and the washing buffer solution 2 in the kit, standing the washing buffer solution 1 at room temperature for later use, and preheating the washing buffer solution 2 at 65 ℃ for more than 5 minutes for later use;
(g) the capture product was placed on a magnetic rack and left for 1 minute until the liquid was clear, and the liquid was carefully aspirated (taking care not to aspirate the beads);
(h) taking the centrifuge tube off the magnetic frame, adding 200 mu L of the cleaning buffer solution 1, blowing and uniformly mixing, rotating the centrifuge tube on a mixing instrument for 15 minutes at the rotating speed of not more than 10 revolutions per minute, standing the centrifuge tube on the magnetic frame for 1 minute until the liquid is clear, and carefully sucking away the liquid (not sucking the magnetic beads);
(i) taking the centrifuge tube off the magnetic frame, adding 200 μ L of 65 ℃ preheated washing buffer 2, blowing and mixing uniformly, placing on a constant temperature shaking instrument (65 ℃, 800rpm) for 10 minutes, placing on the magnetic frame, standing for 1 minute until the liquid is clear, and carefully sucking away the liquid (not sucking the magnetic beads);
(j) repeating the step (4) twice and three times;
(k) keeping the centrifuge tube in a magnetic frame all the time, adding 200 mu L of freshly prepared 80% ethanol, standing for 30 seconds until the liquid is clear, and carefully sucking away the liquid (not sucking magnetic beads);
(l) The reaction tube is left on a magnetic frame to be dried at room temperature for 3-5 minutes to volatilize the residual ethanol, and the surface of the magnetic bead is matt (excessive drying causes the reduction of DNA yield);
(m) removing the centrifuge tube from the magnetic frame, adding 30. mu.L of enzyme-free water, and resuspending the magnetic beads for later use.
(3) Amplification of captured products (on ice)
(a) Mix 3 was prepared in a 0.2mL PCR tube according to the following reaction system: PCR amplification buffer (18. mu.L), PCR amplification primer 2 (1. mu.L), PCR amplification enzyme (1. mu.L);
(b) adding 20 mu L of the reaction system into the product, operating on ice, and fully and uniformly mixing;
(c) placing the mixture in a PCR instrument to perform PCR amplification according to the following reaction procedure, and heating the mixture to 105 ℃ (the PCR reaction conditions in Table 2);
TABLE 2
(4) Purification after amplification
(a) Balancing the purified magnetic beads at room temperature for 30 minutes before use, taking 75 mu L of the purified magnetic beads to 50 mu L of amplification products, carrying out vortex oscillation and uniform mixing, and incubating for 5 minutes at room temperature;
(b) the PCR tube was briefly centrifuged and placed in a magnetic rack to separate the beads and liquid, after the solution cleared (about 5 minutes), the supernatant was carefully removed;
(c) keeping the PCR tube in a magnetic frame all the time, adding 200 mu L of freshly prepared 80% ethanol to rinse the magnetic beads, incubating at room temperature for 30 seconds, carefully removing the supernatant, and repeating the step for 1 time;
(d) keeping the PCR tube in a magnetic frame all the time, opening a cover and drying the magnetic beads in air for 5-10 minutes until no ethanol remains;
(e) add 25. mu.L of enzyme-free water to step 4, vortex, shake and mix well, stand at room temperature for 2 minutes, centrifuge PCR tube briefly and place in magnetic rack and stand, after the solution is clear (about 5 minutes), carefully remove 23. mu.L of supernatant into a new centrifuge tube.
(f) The nucleic acid quantitative Kit Qubit dsDNA HS Assay Kit and a matched instrument are used for carrying out quantification, the library concentration is recorded, the library concentration is more than 1 ng/mu L, the Qsep100 is used for carrying out fragment length measurement, and the library length is about 270bp-400 bp.
(g) Quantification of the on-machine enrichment Library was performed using the KAPA Library Quantification Kit to adjust to the appropriate on-machine concentration, and sequencing was performed using the Illumina sequencing platform.
3. Biological information analysis
(1) After sequencing is completed, converting a BCL file generated by sequencing into a fastq file corresponding to a sample by using BCL2fastq v2.20.0.422 software of an illumina company;
(2) performing quality control on the original data according to the basic groups and the quality in the fastq file (based on fastp v0.20.0 software);
(3) aligning the base sequences in the fastq file to a human reference genome hg38(GRCh38) and an HPV genome to generate a bam file, and sequencing and de-duplicating the sequences by comparison;
(4) counting the numbers and proportions of reads of various HPV subtypes in comparison in a sample;
(5) the HPV integration sites in the sample are analyzed by site comparison of the HPV and human genome sequences in the sequence alignment file, and the positions of the integration sites on the genome are annotated.
Example 3 verification of Using Effect of the high-risk HPV Capture Probe prepared by the method of the invention
The present invention will be described in further detail with reference to the following embodiments.
1. Mixed probes for high-risk HPV DNA were prepared according to the method of example 1.
2. The method of example 2 was used to create a library with sample 1 (sample 1 is a reference: 15 high-risk HPV plasmids + human genomic DNA, each plasmid at a concentration of 10^6copies/mL), sample 2 (Siha cell line DNA known to be integrated by HPV16 infection) and sample 3 (Hela cell line DNA known to be integrated by HPV18 infection); and the high-risk HPV DNA probe group (self-prepared) prepared by the invention and the probe purchased from Aijiekang are used for liquid phase hybridization target capture. Further, high-throughput sequencing and bioinformatics analysis were performed. The results of the sequencing analysis of 3 samples are shown in Table 3 for comparison of the results of the sequencing of the different probes.
TABLE 3
As can be seen from Table 3: the probes prepared by the method and the commercial probes purchased from Aijiekang can accurately detect the types and integration states of 3 samples infected with HPV (sample 1 is positive infection and integration negative infection of HPV16, 18, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66 and 68; sample 2 is positive infection and integration of HPV 16; sample 3 is positive infection and integration of HPV 18), and both meet the capture requirement; meanwhile, the probes prepared by the method capture HPV with equivalent average sequencing depth, capture efficiency and 10X coverage. The probe prepared by the invention can accurately and quickly detect the high-risk HPV infected in the actual sample and the integration state, and has practical use value.
SEQUENCE LISTING
<110> Wuhan Kaideweis medical laboratory Co., Ltd
<120> method for preparing human papilloma virus capture probe set and application thereof
<130> 0
<160> 16
<170> PatentIn version 3.3
<210> 1
<211> 7906
<212> DNA
<213> Artificial Sequence
<220>
<223> Artificial Synthesis
<400> 1
actacaataa ttcatgtata aaactaaggg cgtaaccgaa atcggttgaa ccgaaaccgg 60
ttagtataaa agcagacatt ttatgcacca aaagagaact gcaatgtttc aggacccaca 120
ggagcgaccc agaaagttac cacagttatg cacagagctg caaacaacta tacatgatat 180
aatattagaa tgtgtgtact gcaagcaaca gttactgcga cgtgaggtat atgactttgc 240
ttttcgggat ttatgcatag tatatagaga tgggaatcca tatgctgtat gtgataaatg 300
tttaaagttt tattctaaaa ttagtgagta tagacattat tgttatagtt tgtatggaac 360
aacattagaa cagcaataca acaaaccgtt gtgtgatttg ttaattaggt gtattaactg 420
tcaaaagcca ctgtgtcctg aagaaaagca aagacatctg gacaaaaagc aaagattcca 480
taatataagg ggtcggtgga ccggtcgatg tatgtcttgt tgcagatcat caagaacacg 540
tagagaaacc cagctgtaat catgcatgga gatacaccta cattgcatga atatatgtta 600
gatttgcaac cagagacaac tgatctctac tgttatgagc aattaaatga cagctcagag 660
gaggaggatg aaatagatgg tccagctgga caagcagaac cggacagagc ccattacaat 720
attgtaacct tttgttgcaa gtgtgactct acgcttcggt tgtgcgtaca aagcacacac 780
gtagacattc gtactttgga agacctgtta atgggcacac taggaattgt gtgccccatc 840
tgttctcaga aaccataatc taccatggct gatcctgcag gtaccaatgg ggaagagggt 900
acgggatgta atggatggtt ttatgtagag gctgtagtgg aaaaaaaaac aggggatgct 960
atatcagatg acgagaacga aaatgacagt gatacaggtg aagatttggt agattttata 1020
gtaaatgata atgattattt aacacaggca gaaacagaga cagcacatgc gttgtttact 1080
gcacaggaag caaaacaaca tagagatgca gtacaggttc taaaacgaaa gtatttgggt 1140
agtccactta gtgatattag tggatgtgta gacaataata ttagtcctag attaaaagct 1200
atatgtatag aaaaacaaag tagagctgca aaaaggagat tatttgaaag cgaagacagc 1260
gggtatggca atactgaagt ggaaactcag cagatgttac aggtagaagg gcgccatgag 1320
actgaaacac catgtagtca gtatagtggt ggaagtgggg gtggttgcag tcagtacagt 1380
agtggaagtg ggggagaggg tgttagtgaa agacacacta tatgccaaac accacttaca 1440
aatattttaa atgtactaaa aactagtaat gcaaaggcag caatgttagc aaaatttaaa 1500
gagttatacg gggtgagttt ttcagaatta gtaagaccat ttaaaagtaa taaatcaacg 1560
tgttgcgatt ggtgtattgc tgcatttgga cttacaccca gtatagctga cagtataaaa 1620
acactattac aacaatattg tttatattta cacattcaaa gtttagcatg ttcatgggga 1680
atggttgtgt tactattagt aagatataaa tgtggaaaaa atagagaaac aattgaaaaa 1740
ttgctgtcta aactattatg tgtgtctcca atgtgtatga tgatagagcc tccaaaattg 1800
cgtagtacag cagcagcatt atattggtat aaaacaggta tatcaaatat tagtgaagtg 1860
tatggagaca cgccagaatg gatacaaaga caaacagtat tacaacatag ttttaatgat 1920
tgtacatttg aattatcaca gatggtacaa tgggcctacg ataatgacat agtagacgat 1980
agtgaaattg catataaata tgcacaattg gcagacacta atagtaatgc aagtgccttt 2040
ctaaaaagta attcacaggc aaaaattgta aaggattgtg caacaatgtg tagacattat 2100
aaacgagcag aaaaaaaaca aatgagtatg agtcaatgga taaaatatag atgtgatagg 2160
gtagatgatg gaggtgattg gaagcaaatt gttatgtttt taaggtatca aggtgtagag 2220
tttatgtcat ttttaactgc attaaaaaga tttttgcaag gcatacctaa aaaaaattgc 2280
atattactat atggtgcagc taacacaggt aaatcattat ttggtatgag tttaatgaaa 2340
tttctgcaag ggtctgtaat atgttttgta aattctaaaa gccatttttg gttacaacca 2400
ttagcagatg ccaaaatagg tatgttagat gatgctacag tgccctgttg gaactacata 2460
gatgacaatt taagaaatgc attggatgga aatttagttt ctatggatgt aaagcataga 2520
ccattggtac aactaaaatg ccctccatta ttaattacat ctaacattaa tgctggtaca 2580
gattctaggt ggccttattt acataataga ttggtggtgt ttacatttcc taatgagttt 2640
ccatttgacg aaaacggaaa tccagtgtat gagcttaatg ataagaactg gaaatccttt 2700
ttctcaagga cgtggtccag attaagtttg cacgaggacg aggacaagga aaacgatgga 2760
gactctttgc caacgtttaa atgtgtgtca ggacaaaata ctaacacatt atgaaaatga 2820
tagtacagac ctacgtgacc atatagacta ttggaaacac atgcgcctag aatgtgctat 2880
ttattacaag gccagagaaa tgggatttaa acatattaac caccaggtgg tgccaacact 2940
ggctgtatca aagaataaag cattacaagc aattgaactg caactaacgt tagaaacaat 3000
atataactca caatatagta atgaaaagtg gacattacaa gacgttagcc ttgaagtgta 3060
tttaactgca ccaacaggat gtataaaaaa acatggatat acagtggaag tgcagtttga 3120
tggagacata tgcaatacaa tgcattatac aaactggaca catatatata tttgtgaaga 3180
agcatcagta actgtggtag agggtcaagt tgactattat ggtttatatt atgttcatga 3240
aggaatacga acatattttg tgcagtttaa agatgatgca gaaaaatata gtaaaaataa 3300
agtatgggaa gttcatgcgg gtggtcaggt aatattatgt cctacatctg tgtttagcag 3360
caacgaagta tcctctcctg aaattattag gcagcacttg gccaaccacc ccgccgcgac 3420
ccataccaaa gccgtcgcct tgggcaccga agaaacacag acgactatcc agcgaccaag 3480
atcagagcca gacaccggaa acccctgcca caccactaag ttgttgcaca gagactcagt 3540
ggacagtgct ccaatcctca ctgcatttaa cagctcacac aaaggacgga ttaactgtaa 3600
tagtaacact acacccatag tacatttaaa aggtgatgct aatactttaa aatgtttaag 3660
atatagattt aaaaagcatt gtacattgta tactgcagtg tcgtctacat ggcattggac 3720
aggacataat gtaaaacata aaagtgcaat tgttacactt acatatgata gtgaatggca 3780
acgtgaccaa tttttgtctc aagttaaaat accaaaaact attacagtgt ctactggatt 3840
tatgtctata tgacaaatct tgatactgca tccacaacat tactggcgtg ctttttgctt 3900
tgcttttgtg tgcttttgtg tgtctgccta ttaatacgtc cgctgctttt gtctgtgtct 3960
acatacacat cattaataat attggtatta ctattgtgga taacagcagc ctctgcgttt 4020
aggtgtttta ttgtatatat tatatttgtt tatataccat tatttttaat acatacacat 4080
gcacgctttt taattacata atgtatatgt acataatgta attgttacat ataattgttg 4140
tataccataa cttactattt tttctttttt attttcatat ataatttttt tttttgtttg 4200
tttgtttgtt ttttaataaa ctgttattac ttaacaatgc gacacaaacg ttctgcaaaa 4260
cgcacaaaac gtgcatcggc tacccaactt tataaaacat gcaaacaggc aggtacatgt 4320
ccacctgaca ttatacctaa ggttgaaggc aaaactattg ctgatcaaat attacaatat 4380
ggaagtatgg gtgtattttt tggtgggtta ggaattggaa cagggtcggg tacaggcgga 4440
cgcactgggt atattccatt gggaacaagg cctcccacag ctacagatac acttgctcct 4500
gtaagacccc ctttaacagt agatcctgtg ggcccttctg atccttctat agtttcttta 4560
gtggaagaaa ctagttttat tgatgctggt gcaccaacat ctgtaccttc cattccccca 4620
gatgtatcag gatttagtat tactacttca actgatacca cacctgctat attagatatt 4680
aataatactg ttactactgt tactacacat aataatccca ctttcactga cccatctgta 4740
ttgcagcctc caacacctgc agaaactgga gggcatttta cactttcatc atccactatt 4800
agtacacata attatgaaga aattcctatg gatacattta ttgttagcac aaaccctaac 4860
acagtaacta gtagcacacc cataccaggg tctcgcccag tggcacgcct aggattatat 4920
agtcgcacaa cacaacaggt taaagttgta gaccctgctt ttgtaaccac tcccactaaa 4980
cttattacat atgataatcc tgcatatgaa ggtatagatg tggataatac attatatttt 5040
tctagtaatg ataatagtat taatatagct ccagatcctg actttttgga tatagttgct 5100
ttacataggc cagcattaac ctctaggcgt actggcatta ggtacagtag aattggtaat 5160
aaacaaacac tacgtactcg tagtggaaaa tctataggtg ctaaggtaca ttattattat 5220
gatttaagta ctattgatcc tgcagaagaa atagaattac aaactataac accttctaca 5280
tatactacca cttcacatgc agcctcacct acttctatta ataatggatt atatgatatt 5340
tatgcagatg actttattac agatacttct acaaccccgg taccatctgt accctctaca 5400
tctttatcag gttatattcc tgcaaataca acaattcctt ttggtggtgc atacaatatt 5460
cctttagtat caggtcctga tatacccatt aatataactg accaagctcc ttcattaatt 5520
cctatagttc cagggtctcc acaatataca attattgctg atgcaggtga cttttattta 5580
catcctagtt attacatgtt acgaaaacga cgtaaacgtt taccatattt tttttcagat 5640
gtctctttgg ctgcctagtg aggccactgt ctacttgcct cctgtcccag tatctaaggt 5700
tgtaagcacg gatgaatatg ttgcacgcac aaacatatat tatcatgcag gaacatccag 5760
actacttgca gttggacatc cctattttcc tattaaaaaa cctaacaata acaaaatatt 5820
agttcctaaa gtatcaggat tacaatacag ggtatttaga atacatttac ctgaccccaa 5880
taagtttggt tttcctgaca cctcatttta taatccagat acacagcggc tggtttgggc 5940
ctgtgtaggt gttgaggtag gtcgtggtca gccattaggt gtgggcatta gtggccatcc 6000
tttattaaat aaattggatg acacagaaaa tgctagtgct tatgcagcaa atgcaggtgt 6060
ggataataga gaatgtatat ctatggatta caaacaaaca caattgtgtt taattggttg 6120
caaaccacct ataggggaac actggggcaa aggatcccca tgtaccaatg ttgcagtaaa 6180
tccaggtgat tgtccaccat tagagttaat aaacacagtt attcaggatg gtgatatggt 6240
tgatactggc tttggtgcta tggactttac tacattacag gctaacaaaa gtgaagttcc 6300
actggatatt tgtacatcta tttgcaaata tccagattat attaaaatgg tgtcagaacc 6360
atatggcgac agcttatttt tttatttacg aagggaacaa atgtttgtta gacatttatt 6420
taatagggct ggtactgttg gtgaaaatgt accagacgat ttatacatta aaggctctgg 6480
gtctactgca aatttagcca gttcaaatta ttttcctaca cctagtggtt ctatggttac 6540
ctctgatgcc caaatattca ataaacctta ttggttacaa cgagcacagg gccacaataa 6600
tggcatttgt tggggtaacc aactatttgt tactgttgtt gatactacac gcagtacaaa 6660
tatgtcatta tgtgctgcca tatctacttc agaaactaca tataaaaata ctaactttaa 6720
ggagtaccta cgacatgggg aggaatatga tttacagttt atttttcaac tgtgcaaaat 6780
aaccttaact gcagacgtta tgacatacat acattctatg aattccacta ttttggagga 6840
ctggaatttt ggtctacaac ctcccccagg aggcacacta gaagatactt ataggtttgt 6900
aacatcccag gcaattgctt gtcaaaaaca tacacctcca gcacctaaag aagatcccct 6960
taaaaaatac actttttggg aagtaaattt aaaggaaaag ttttctgcag acctagatca 7020
gtttccttta ggacgcaaat ttttactaca agcaggattg aaggccaaac caaaatttac 7080
attaggaaaa cgaaaagcta cacccaccac ctcatctacc tctacaactg ctaaacgcaa 7140
aaaacgtaag ctgtaagtat tgtatgtatg ttgaattagt gttgtttgtt gtgtatatgt 7200
ttgtatgtgc ttgtatgtgc ttgtaaatat taagttgtat gtgtgtttgt atgtatggta 7260
taataaacac gtgtgtatgt gtttttaaat gcttgtgtaa ctattgtgtc atgcaacata 7320
aataaactta ttgtttcaac acctactaat tgtgttgtgg ttattcattg tatataaact 7380
atatttgcta catcctgttt ttgttttata tatactatat tttgtagcgc cagcggccat 7440
tttgtagctt caaccgaatt cggttgcatg ctttttggca caaaatgtgt ttttttaaat 7500
agttctatgt cagcaactat ggtttaaact tgtacgtttc ctgcttgcca tgcgtgccaa 7560
atccctgttt tcctgacctg cactgcttgc caaccattcc attgtttttt acactgcact 7620
atgtgcaact actgaatcac tatgtacatt gtgtcatata aaataaatca ctatgcgcca 7680
acgccttaca taccgctgtt aggcacatat ttttggcttg ttttaactaa cctaattgca 7740
tatttggcat aaggtttaaa cttctaaggc caactaaatg tcaccctagt tcatacatga 7800
actgtgtaaa ggttagtcat acattgttca tttgtaaaac tgcacatggg tgtgtgcaaa 7860
ccgttttggg ttacacattt acaagcaact tatataataa tactaa 7906
<210> 2
<211> 7857
<212> DNA
<213> Artificial Sequence
<220>
<223> Artificial Synthesis
<400> 2
attaatactt ttaacaattg tagtatataa aaaagggagt aaccgaaaac ggtcgggacc 60
gaaaacggtg tatataaaag atgtgagaaa cacaccacaa tactatggcg cgctttgagg 120
atccaacacg gcgaccctac aagctacctg atctgtgcac ggaactgaac acttcactgc 180
aagacataga aataacctgt gtatattgca agacagtatt ggaacttaca gaggtatttg 240
aatttgcatt taaagattta tttgtggtgt atagagacag tataccgcat gctgcatgcc 300
ataaatgtat agatttttat tctagaatta gagaattaag acattattca gactctgtgt 360
atggagacac attggaaaaa ctaactaaca ctgggttata caatttatta ataaggtgcc 420
tgcggtgcca gaaaccgttg aatccagcag aaaaacttag acaccttaat gaaaaacgac 480
gatttcacaa catagctggg cactatagag gccagtgcca ttcgtgctgc aaccgagcac 540
gacaggaacg actccaacga cgcagagaaa cacaagtata atattaagta tgcatggacc 600
taaggcaaca ttgcaagaca ttgtattgca tttagagccc caaaatgaaa ttccggttga 660
ccttctatgt cacgagcaat taagcgactc agaggaagaa aacgatgaaa tagatggagt 720
taatcatcaa catttaccag cccgacgagc cgaaccacaa cgtcacacaa tgttgtgtat 780
gtgttgtaag tgtgaagcca gaattgagct agtagtagaa agctcagcag acgaccttcg 840
agcattccag cagctgtttc tgaacaccct gtcctttgtg tgtccgtggt gtgcatccca 900
gcagtaagca acaatggctg atccagaagg tacagacggg gagggcacgg gttgtaacgg 960
ctggttttat gtacaagcta ttgtagacaa aaaaacagga gatgtaatat cagatgacga 1020
ggacgaaaat gcaacagaca cagggtcgga tatggtagat tttattgata cacaaggaac 1080
attttgtgaa caggcagagc tagagacagc acaggcattg ttccatgcgc aggaggtcca 1140
caatgatgca caagtgttgc atgttttaaa acgaaagttt gcaggaggca gcacagaaaa 1200
cagtccatta ggggagcggc tggaggtgga tacagagtta agtccacggt tacaagaaat 1260
atctttaaat agtgggcaga aaaaggcaaa aaggcggctg tttacaatat cagatagtgg 1320
ctatggctgt tctgaagtgg aagcaacaca gattcaggta actacaaatg gcgaacatgg 1380
cggcaatgta tgtagtggcg gcagtacgga ggctatagac aacgggggca cagagggcaa 1440
caacagcagt gtagacggta caagtgacaa tagcaatata gaaaatgtaa atccacaatg 1500
taccatagca caattaaaag acttgttaaa agtaaacaat aaacaaggag ctatgttagc 1560
agtatttaaa gacacatatg ggctatcatt tacagattta gttagaaatt ttaaaagtga 1620
taaaaccacg tgtacagatt gggttacagc tatatttgga gtaaacccaa caatagcaga 1680
aggatttaaa acactaatac agccatttat attatatgcc catattcaat gtctagactg 1740
taaatgggga gtattaatat tagccctgtt gcgttacaaa tgtggtaaga gtagactaac 1800
agttgctaaa ggtttaagta cgttgttaca cgtacctgaa acttgtatgt taattcaacc 1860
accaaaattg cgaagtagtg ttgcagcact atattggtat agaacaggaa tatcaaatat 1920
tagtgaagta atgggagaca cacctgagtg gatacaaaga cttactatta tacaacatgg 1980
aatagatgat agcaattttg atttgtcaga aatggtacaa tgggcatttg ataatgagct 2040
gacagatgaa agcgatatgg catttgaata tgccttatta gcagacagca acagcaatgc 2100
agctgccttt ttaaaaagca attgccaagc taaatattta aaagattgtg ccacaatgtg 2160
caaacattat aggcgagccc aaaaacgaca aatgaatatg tcacagtgga tacgatttag 2220
atgttcaaaa atagatgaag ggggagattg gagaccaata gtgcaattcc tgcgatacca 2280
acaaatagag tttataacat ttttaggagc cttaaaatca tttttaaaag gaacccccaa 2340
aaaaaattgt ttagtatttt gtggaccagc aaatacagga aaatcatatt ttggaatgag 2400
ttttatacac tttatacaag gagcagtaat atcatttgtg aattccacta gtcatttttg 2460
gttggaaccg ttaacagata ctaaggtggc catgttagat gatgcaacga ccacgtgttg 2520
gacatacttt gatacctata tgagaaatgc gttagatggc aatccaataa gtattgatag 2580
aaagcacaaa ccattaatac aactaaaatg tcctccaata ctactaacca caaatataca 2640
tccagcaaag gataatagat ggccatattt agaaagtaga ataacagtat ttgaatttcc 2700
aaatgcattt ccatttgata aaaatggcaa tccagtatat gaaataaatg acaaaaattg 2760
gaaatgtttt tttgaaagga catggtccag attagatttg cacgaggaag aggaagatgc 2820
agacaccgaa ggaaaccctt tcggaacgtt taagtgcgtt gcaggacaaa atcatagacc 2880
actatgaaaa tgacagtaaa gacatagaca gccaaataca gtattggcaa ctaatacgtt 2940
gggaaaatgc aatattcttt gcagcaaggg aacatggcat acagacatta aaccaccagg 3000
tggtgccagc ctataacatt tcaaaaagta aagcacataa agctattgaa ctgcaaatgg 3060
ccctacaagg ccttgcacaa agtgcataca aaaccgagga ttggacactg caagacacat 3120
gcgaggaact atggaataca gaacctactc actgctttaa aaaaggtggc caaacagtac 3180
aagtatattt tgatggcaac aaagacaatt gtatgaccta tgtagcatgg gacagtgtgt 3240
attatatgac tgatgcagga acatgggaca aaacggctac ctgtgtaagt cacaggggat 3300
tgtattatgt aaaggaaggg tacaacacgt tttatataga atttaaaagt gaatgtgaaa 3360
aatatgggaa cacaggtacg tgggaagtac attttgggaa taatgtaatt gattgtaatg 3420
actctatgtg cagtaccagt gacgacacgg tatccgctac tcagcttgtt aaacagctac 3480
agcacacccc ctcaccgtat tccagcaccg tgtccgtggg caccgcaaag acctacggcc 3540
agacgtcggc tgctacacga cctggacact gtggactcgc ggagaagcag cattgtggac 3600
ctgtcaaccc acttctcggt gcagctacac ctacaggcaa caacaaaaga cggaaactct 3660
gtagtggtaa cactacgcct ataatacatt taaaaggtga cagaaacagt ttaaaatgtt 3720
tacggtacag attgcgaaaa catagcgacc actatagaga tatatcatcc acctggcatt 3780
ggacaggtgc aggcaatgaa aaaacaggaa tactgactgt aacataccat agtgaaacac 3840
aaagaacaaa atttttaaat actgttgcaa ttccagatag tgtacaaata ttggtgggat 3900
acatgacaat gtaatacata tgctgtagta ccaatatgtt atcacttatt tttttatttt 3960
gcttttgtgt atgcatgtat gtgtgctgcc atgtcccgct tttgccatct gtctgtatgt 4020
gtgcgtatgc atgggtattg gtatttgtgt atattgtggt aataacgtcc cctgccacag 4080
cattcacagt atatgtattt tgttttttat tgcccatgtt actattgcat atacatgcta 4140
tattgtcttt acagtaattg tataggttgt tttatacagt gtattgtaca ttgtatattt 4200
tgttttatac cttttatgct ttttgtattt ttgtaataaa agtatggtat cccaccgtgc 4260
cgcacgacgc aaacgggctt cggtaactga cttatataaa acatgtaaac aatctggtac 4320
atgtccacct gatgttgttc ctaaggtgga gggcaccacg ttagcagata aaatattgca 4380
atggtcaagc cttggtatat ttttgggtgg acttggcata ggtactggca gtggtacagg 4440
gggtcgtaca gggtacattc cattgggtgg gcgttccaat acagtggtgg atgttggtcc 4500
tacacgtccc ccagtggtta ttgaacctgt gggccccaca gacccatcta ttgttacatt 4560
aatagaggac tccagtgtgg ttacatcagg tgcacctagg cctacgttta ctggcacgtc 4620
tgggtttgat ataacatctg cgggtacaac tacacctgcg gttttggata tcacaccttc 4680
gtctacctct gtgtctattt ccacaaccaa ttttaccaat cctgcatttt ctgatccgtc 4740
cattattgaa gttccacaaa ctggggaggt ggcaggtaat gtatttgttg gtacccctac 4800
atctggaaca catgggtatg aggaaatacc tttacaaaca tttgcttctt ctggtacggg 4860
ggaggaaccc attagtagta ccccattgcc tactgtgcgg cgtgtagcag gtccccgcct 4920
ttacagtagg gcctaccaac aagtgtcagt ggctaaccct gagtttctta cacgtccatc 4980
ctctttaatt acatatgaca acccggcctt tgagcctgtg gacactacat taacatttga 5040
tcctcgtagt gatgttcctg attcagattt tatggatatt atccgtctac ataggcctgc 5100
tttaacatcc aggcgtggga ctgttcgctt tagtagatta ggtcaacggg caactatgtt 5160
tacccgcagc ggtacacaaa taggtgctag ggttcacttt tatcatgata taagtcctat 5220
tgcaccttcc ccagaatata ttgaactgca gcctttagta tctgccacgg aggacaatga 5280
cttgtttgat atatatgcag atgacatgga ccctgcagtg cctgtaccat cgcgttctac 5340
tacctccttt gcatttttta aatattcgcc cactatatct tctgcctctt cctatagtaa 5400
tgtaacggtc cctttaacct cctcttggga tgtgcctgta tacacgggtc ctgatattac 5460
attaccatct actacctctg tatggcccat tgtatcaccc acggcccctg cctctacaca 5520
gtatattggt atacatggta cacattatta tttgtggcca ttatattatt ttattcctaa 5580
gaaacgtaaa cgtgttccct atttttttgc agatggcttt gtggcggcct agtgacaata 5640
ccgtatatct tccacctcct tctgtggcaa gagttgtaaa taccgatgat tatgtgactc 5700
gcacaagcat attttatcat gctggcagct ctagattatt aactgttggt aatccatatt 5760
ttagggttcc tgcaggtggt ggcaataagc aggatattcc taaggtttct gcataccaat 5820
atagagtatt tagggtgcag ttacctgacc caaataaatt tggtttacct gatactagta 5880
tttataatcc tgaaacacaa cgtttagtgt gggcctgtgc tggagtggaa attggccgtg 5940
gtcagccttt aggtgttggc cttagtgggc atccatttta taataaatta gatgacactg 6000
aaagttccca tgccgccacg tctaatgttt ctgaggacgt tagggacaat gtgtctgtag 6060
attataagca gacacagtta tgtattttgg gctgtgcccc tgctattggg gaacactggg 6120
ctaaaggcac tgcttgtaaa tcgcgtcctt tatcacaggg cgattgcccc cctttagaac 6180
ttaaaaacac agttttggaa gatggtgata tggtagatac tggatatggt gccatggact 6240
ttagtacatt gcaagatact aaatgtgagg taccattgga tatttgtcag tctatttgta 6300
aatatcctga ttatttacaa atgtctgcag atccttatgg ggattccatg tttttttgct 6360
tacggcgtga gcagcttttt gctaggcatt tttggaatag agcaggtact atgggtgaca 6420
ctgtgcctca atccttatat attaaaggca caggtatgcg tgcttcacct ggcagctgtg 6480
tgtattctcc ctctccaagt ggctctattg ttacctctga ctcccagttg tttaataaac 6540
catattggtt acataaggca cagggtcata acaatggtgt ttgctggcat aatcaattat 6600
ttgttactgt ggtagatacc actcgcagta ccaatttaac aatatgtgct tctacacagt 6660
ctcctgtacc tgggcaatat gatgctacca aatttaagca gtatagcaga catgttgagg 6720
aatatgattt gcagtttatt tttcagttgt gtactattac tttaactgca gatgttatgt 6780
cctatattca tagtatgaat agcagtattt tagaggattg gaactttggt gttccccccc 6840
cgccaactac tagtttggtg gatacatatc gttttgtaca atctgttgct attacctgtc 6900
aaaaggatgc tgcaccggct gaaaataagg atccctatga taagttaaag ttttggaatg 6960
tggatttaaa ggaaaagttt tctttagact tagatcaata tccccttgga cgtaaatttt 7020
tggttcaggc tggattgcgt cgcaagccca ccataggccc tcgcaaacgt tctgctccat 7080
ctgccactac gtcttctaaa cctgccaagc gtgtgcgtgt acgtgccagg aagtaatatg 7140
tgtgtgtgta tatatatata catctattgt tgtgtttgta tgtcctgtgt ttgtgtttgt 7200
tgtatgattg cattgtatgg tatgtatggt tgttgttgta tgttgtatgt tactatattt 7260
gttggtatgt ggcattaaat aaaatatgtt ttgtggttct gtgtgttatg tggttgcgcc 7320
ctagtgagta acaactgtat ttgtgtttgt ggtatgggtg ttgcttgttg ggctatatat 7380
tgtcctgtat ttcaagttat aaaactgcac accttacagc atccatttta tcctacaatc 7440
ctccattttg ctgtgcaacc gatttcggtt gcctttggct tatgtctgtg gttttctgca 7500
caatacagta cgctggcact attgcaaact ttaatctttt gggcactgct cctacatatt 7560
ttgaacaatt ggcgcgcctc tttggcgcat ataaggcgca cctggtatta gtcattttcc 7620
tgtccaggtg cgctacaaca attgcttgca taactatatc cactccctaa gtaataaaac 7680
tgcttttagg cacatatttt agtttgtttt tacttaagct aattgcatac ttggcttgta 7740
caactacttt catgtccaac attctgtcta cccttaacat gaactataat atgactaagc 7800
tgtgcataca tagtttatgc aaccgaaata ggttgggcag cacatactat acttttc 7857
<210> 3
<211> 7912
<212> DNA
<213> Artificial Sequence
<220>
<223> Artificial Synthesis
<400> 3
taataataat aatcttagta taaaaaagta gggagtgacc gaaagtggtg aaccgaaaac 60
ggttggtata taaagcacat agtattttgt gcaaacctac agacgccatg ttcaaaaatc 120
ctgcagaaag acctcggaaa ttgcatgaac taagctcggc attggaaata ccctacgatg 180
aactaagatt gaattgtgtc tactgcaaag gtcagttaac agaaacagag gtattagatt 240
ttgcatttac agatttaaca atagtatata gggacgacac accacacgga gtgtgtacaa 300
aatgtttaag attttattca aaagtaagtg aatttagatg gtatagatat agtgtgtatg 360
gaacaacatt agaaaaattg acaaacaaag gtatatgtga tttgttaatt aggtgtataa 420
cgtgtcaaag accgttgtgt ccagaagaaa aacaaagaca tttggataaa aagaaacgat 480
tccacaacat aggaggaagg tggacaggac gttgcatagc atgttggaga agacctcgta 540
ctgaaaccca agtgtaaaca tgcgtggaga aacacctacg ttgcaagact atgtgttaga 600
tttgcaacct gaggcaactg acctccactg ttatgagcaa ttacccgaca gctcagatga 660
ggaggatgtc atagacagtc cagctggaca agcagaaccg gacacatcca attacaatat 720
cgttaccttt tgttgtcagt gtaagtctac acttcgtttg tgtgtacaga gcacacaagt 780
agatattcgc atattgcaag agctgttaat gggctcattt ggaatcgtgt gccccaactg 840
ttctactaga ctgtaactac aatggctgat ccagcaggta cagatgggga ggggacggga 900
tgcaatggtt ggttttatgt agaagcagta attgacagac agacagggga caacatttca 960
gaggacgaaa atgaagacag tagtgatact ggggaggata tggttgactt tattgacaat 1020
tgtaatgtat acaacaatca ggcagaagca gagacagcac aggcattgtt tcatgcacag 1080
gaagcggagg aacatgcaga ggctgtgcag gttctaaaac gaaagtatgt aggtagtcct 1140
ttaagtgata ttagtagttg tgtggattat aatattagtc cacggttaaa agctatatgc 1200
atagaaaata acagtaaaac agcaaaacga agactctttg aacttccaga cagcgggtat 1260
ggcaatactg aagtggaaac gcagcagatg gtacaggtag aggagcaaca aacaacatta 1320
agttgtaatg gtagtgacgg gacacatagt gaacgagaga atgaaactcc aacacgtaat 1380
atattgcaag tgttaaaaac tagcaatggt aaagctgcta tgttaggtaa atttaaagaa 1440
ttatatggtg taagttttat ggaactaatt aggccatttc aaagcaataa aagcacatgt 1500
actgattggt gtgtagctgc gtttggagtt acaggtacag ttgcagaagg atttaaaacc 1560
ctattgcaac catattgttt gtattgccat ttacaaagtt tagcatgttc ctggggcatg 1620
gttatgttaa tgcttgtgag atttaaatgt gcaaaaaata gaataacaat tgaaaaatta 1680
ttagaaaaat tattgtgtat atctacaaat tgtatgttaa ttcagccacc caaattacgt 1740
agcacagctg cagcattata ttggtacaga acaggaatgt caaacattag cgatgtatat 1800
ggtgaaacac cagaatggat agaaagacaa acagtattac agcatagttt taatgacaca 1860
acatttgatt tgtcccaaat ggtacaatgg gcatatgaca atgatgttat ggatgatagt 1920
gaaattgcct ataaatatgc acaattagct gacagtgata gtaatgcatg tgcattttta 1980
aaaagtaatt cgcaggcaaa aatagttaaa gattgtggaa caatgtgtag acattataaa 2040
cgagcagaaa aacgacaaat gtccatggga cagtggatta aaagtagatg tgacaaagtt 2100
agtgacgaag gtgactggag ggacatagta aagtttttaa gatatcaaca aatagaattt 2160
gtgtcatttt tatctgcatt aaagctgttt ttaaaaggag tgccaaagaa aaactgtatt 2220
ttaatacatg gtgcacctaa tacaggtaaa tcatattttg gaatgagcct tattagcttt 2280
ttacaaggat gtataatatc atatgcaaat tcaaaaagtc atttttggtt acaaccactg 2340
gctgatgcta aaataggcat gttagatgat gctacaacgc catgttggca ttatatagac 2400
aattacctac gaaatgcact agatggcaac cctgtatcta tagatgtaaa gcataaagct 2460
ttaatgcagt taaaatgtcc tcctttattg attacatcta atataaatgc aggtaaggat 2520
gacagatggc catacctaca tagcagactg gtggttttta catttccaaa tccatttcca 2580
tttgacaaaa acggaaatcc agtatatgaa ttaagtgata aaaactggaa atcctttttc 2640
tcaaggacgt ggtgcagatt aaatttgcac gaggaagagg acaaagaaaa cgatggagac 2700
tctttctcaa cgtttaaatg tgtgtcagga caaaatatta gaacattatg aaaatgatag 2760
taaacgactt tgtgatcata tagactattg gaaacatatt cgacttgaat gtgtattaat 2820
gtataaagca agagaaatgg gaatacacag tattaaccac caggtggtgc cagcgttgtc 2880
agtatcaaag gccaaagcct tacaagctat tgaactacaa atgatgttgg aaacattaaa 2940
taacactgaa tacaaaaatg aggactggac aatgcagcaa acaagtcttg aactgtattt 3000
aactgcacct acagggtgtt taaaaaaaca tggatatact gtagaggtgc aatttgatgg 3060
tgatgtacac aacaccatgc attatactaa ctggaaattt atatacctat gtatagatgg 3120
ccaatgtact gttgtggaag ggcaagttaa ttgtaagggc atttattatg tacatgaagg 3180
acatataaca tattttgtaa attttacaga agaggcaaaa aaatatggga ctggtaaaaa 3240
atgggaagtg catgcgggtg gtcaggtaat tgtttttcct gaatctgtat ttagcagtga 3300
cgaaatatcc tttgctggga ttgttacaaa gctaccaaca gccaacaaca ccaccacatc 3360
gaattccaaa acctgcgcct tgggcaccag tgaaggtgtg cggcgggcga cgacgtctac 3420
taagcgacca agaacagagc cagagcacag aaacacccac caccccaaca agttgttgcg 3480
aggcgactcc gtggacagtg tcaactgtgg ggttatcagt gcagctgcat gcacaaacca 3540
aacaagggct gtcagttgtc ctgcaactac acctataata cacttaaaag gtgatgcaaa 3600
tatattaaaa tgtttaagat ataggctgtc aaaatataaa caattgtatg aacaagtgtc 3660
atctacatgg cattggacat gtacagatgg aaaacataaa aatgctattg taaccttaac 3720
atatataagt acatcacaaa gagacgattt tttaaatact gtaaaaatac ctaacacagt 3780
atcagtgtca acaggatata tgactattta gcctaatgat tgaactaaat atttctacag 3840
taagcattgt gctatgcttt ttgctttgct tttgtgtgct actatttgtg tgtcttgtca 3900
tacgtccact tgtgctgtct gtgtcggtat atgcaacact actattatta attgtgattt 3960
tatgggttat tgcaacctct ccattacgtt gtttttgtat atatgttgtg tttatatata 4020
ttccattatt tgtaattcat acacatgcat cttttttaag tcaacagtaa cttttttact 4080
tgtgtatact gttgtttgta ttggtattgg tattggtatt ggtattggta ttggtataat 4140
aaactttttt actttttttt tattattacc atgcggtcca aacgctctac aaaacgcact 4200
aaacgtgcgt ctgctacaca attatatcaa acatgtaaag cagcaggtac ttgtccatca 4260
gacgttatac ctaaaataga acatactacc attgcagacc aaatattaag gtatggtagt 4320
atgggtgttt tttttggtgg gttgggtatt gggtccggct ctggtactgg gggtcgcact 4380
ggatatgtcc ctcttagtac acgtccttct acagtatctg aggcaagtat acctattaga 4440
ccaccagtta gcattgaccc tgtaggtccc ttggacccct ctatagtaag tcttgttgaa 4500
gaatctggaa ttgttgatgt tggtgcccct gctcctatac cacaccctcc tacaacatct 4560
gggtttgaca ttgctacaac tgcagacaca acacctgcaa ttttagatgt aacaagtgtt 4620
agcacacatg aaaatcctac ttttactgat ccatctgtat tgcagcctcc tacacctgca 4680
gaaacatcag gtcatttact actttcatca tcatctatta gcacacataa ttatgaggaa 4740
atacctatgg atacatttat tgtttctact aataatgaaa acataacaag tagcacaccc 4800
attccagggg tgcgccgtcc tgcacgttta gggttatata gtaaggctac acaacaagta 4860
aaagttattg atccaacgtt tcttagtgct ccaaaacagc taattacata tgaaaaccct 4920
gcctatgaaa ctgtaaatgc tgaagaatct ttatactttt ccaatacatc gcataatata 4980
gcccctgatc ccgactttct agatattata gcattacata ggcctgccct tacctcacgt 5040
aggaacactg ttagatatag tagactaggt aataaacaaa ctttgcgcac tcgtagtggt 5100
gctactattg gtgcaagggt gcattattat tatgatatta gtagtattaa tcctgcaggt 5160
gaaagtattg aaatgcaacc tttaggggcg tctgcaacta ctacttctac tttaaatgat 5220
ggcttatatg acatttatgc agacactgat tttactgtgg atacacctgc cacacataat 5280
gtttcccctt ctactgctgt acagtccaca tctgctgtgt ctgcctatgt acctacaaat 5340
accactgtgc cactaagtac aggttttgac attcccatat tttctgggcc tgatgtacct 5400
atagagcatg cacctacaca ggttttccca tttcctttgg cccctacaac gccacaagtg 5460
tctatttttg ttgatggggg tgatttttat ttgcacccta gttattatat gttaaaacgt 5520
cgacgtaaac gtgtatcata tttttttaca gatgtctctg tggcggccta gcgaggctac 5580
tgtctactta ccacctgtcc cagtgtctaa agttgtaagc acggatgaat atgtaacacg 5640
aaccaacata tattatcacg caggcagtgc taggctgctt acagtaggcc atccatatta 5700
ttccatacct aaatctgaca atcctaaaaa aatagttgta ccaaaggtgt caggattaca 5760
atatagggta tttagggttc gtttaccaga tccaaacaaa tttggatttc ctgatacatc 5820
tttttataat cctgaaactc aacgcttagt ttgggcctgt gttggtttag aggtaggtcg 5880
cgggcagcca ttaggtgtag gtattagtgg tcatccatta ttaaataaat ttgatgacac 5940
tgaaaactct aatagatatg ccggtggtcc tggcactgat aatagggaat gtatatcaat 6000
ggattataaa caaacacaac tgtgtttact tggttgcaaa ccacctattg gagagcattg 6060
gggtaaaggt agtccttgta gtaacaatgc tattacccct ggtgattgtc ctccattaga 6120
attaaaaaat tcagttatac aagatgggga tatggttgat acaggctttg gagctatgga 6180
ttttactgct ttacaagaca ctaaaagtaa tgttcctttg gacatttgta attctatttg 6240
taaatatcca gattatctta aaatggttgc tgagccatat ggcgatacat tattttttta 6300
tttacgtagg gaacaaatgt ttgtaaggca tttttttaat agatcaggca cggttggtga 6360
atcggtccct actgacttat atattaaagg ctccggttca acagctactt tagctaacag 6420
tacatacttt cctacaccta gcggctccat ggttacttca gatgcacaaa tttttaataa 6480
accatattgg atgcaacgtg ctcagggaca caataatggt atttgttggg gcaatcagtt 6540
atttgttact gtggtagata ccacacgtag taccaatatg tctgtttgtg ctgcaattgc 6600
aaacagtgat actacattta aaagtagtaa ttttaaagag tatttaagac atggtgagga 6660
atttgattta caatttatat ttcagttatg caaaataaca ttatctgcag acataatgac 6720
atatattcac agtatgaatc ctgctatttt ggaagattgg aattttggat tgaccacacc 6780
tccctcaggt tctttggagg atacctatag gtttgtcacc tcacaggcca ttacatgtca 6840
aaaaactgcc ccccaaaagc ccaaggaaga tccatttaaa gattatgtat tttgggaggt 6900
taatttaaaa gaaaagtttt ctgcagattt agatcagttt ccactgggtc gcaaattttt 6960
attacaggca ggatataggg cacgtcctaa atttaaagca ggtaaacgta gtgcaccctc 7020
agcatctacc actacaccag caaaacgtaa aaaaactaaa aagtaatgga tgtgtatgta 7080
atacatgtgt ctgtatgtgt atgtgcttgt gctgtattgt atatgtgtgt gtttgtgtgt 7140
tatatatggt atatgtatgt ttatgtatgc gtgtgtactt gtatatatgt atagtatgtt 7200
atgtgtgtat gtatgctatg tatgttaata aatatgtgta tacctgtgtg tgttgtgtat 7260
gttgtcctta tatacaccct attagtaaca tactattact attttataaa ctattgttcc 7320
tacttgttcc tacttgttcc tgctcctccc aatagtcatg tacttatttc tgcctataat 7380
ttaggtgtca cgccatagta aaagttgtac acccggtccg ttttttgcaa ctaaagctac 7440
tccattttga ttttatgcag ccattttaaa tccctaaccg ttttcggttg cattgtttaa 7500
acatgctagt acaactatgc tgatgcagta gttctgcggt ttttggtttc ctgaatacta 7560
gtttttgcca acattctggc ttgtagtttc ctgcctaaca caccttgcca acatataatc 7620
cagtccaact ttgcaattat actatgaatc atgtttgttt aaatacaact gtagttcaac 7680
tatgtgtcat gcacatatat tatattatcc tacacacctt aaactgcttt taggcacata 7740
ttttgtagat tatctatatc cttgattgca gtgctggctt ttgcacatgt ttaaactgcc 7800
aaggttgtgt catgcattat aaataagttg tatgttactc atataattaa ttgcatatag 7860
gtattacacc gttttcggtt acagttttac aagcaattgt tctttttata ct 7912
<210> 4
<211> 7909
<212> DNA
<213> Artificial Sequence
<220>
<223> Artificial Synthesis
<400> 4
gtaaactata atgccaagtt ttaaaaaagt agggtgtaac cgaaagcggt tcaaccgaaa 60
acggtgcata tataaagcaa acattttgca gtaaggtact gcacgactat gtttcaagac 120
actgaggaaa aaccacgaac attgcatgat ttgtgccaag cattggagac aactatacac 180
aacattgaac tacagtgcgt ggaatgcaaa aaacctttgc aacgatctga ggtatatgat 240
tttgcatttg cagatttaac agttgtatat agagagggaa atccatttgg aatatgtaaa 300
ctgtgtttgc ggttcttatc taaaattagt gaatatagac attataatta ttctgtatat 360
ggaaatacat tagaacaaac agttaaaaaa cctttaaatg aaatattaat taggtgtatt 420
atatgtcaaa gacctttgtg tcctcaagaa aaaaaacgac atgtggattt aaacaaacga 480
tttcataata tttcgggtcg ttgggcaggg cgctgtgcgg cgtgttggag gtcccgacgt 540
agagaaactg cactgtgacg tgtaaaaacg ccatgagagg acacaagcca acgttaaagg 600
aatatgtttt agatttatat cctgaaccaa ctgacctata ctgctatgag caattaagtg 660
acagctcaga tgaggatgaa ggcttggacc ggccagatgg acaagcacaa ccagccacag 720
ctgattacta cattgtaacc tgttgtcaca cttgtaacac cacagttcgt ttatgtgtca 780
acagtacagc aagtgaccta cgaaccatac agcaactact tatgggcaca gtgaatattg 840
tgtgccctac ctgtgcacaa caataaacat catctacaat ggccgatcct gaaggtacaa 900
atggggctgg gatggggtgt actggttggt ttgaggtaga agcagtcata gagagaagaa 960
caggagataa tatttcagaa gatgaggatg aaacagcaga tgacagtggc acggatttac 1020
tagagtttat agatgattct atggaaaata gtatacaggc agacacagag gcagcccggg 1080
cattgtttaa tatacaggaa ggggaggatg atttaaatgc tgtgtgtgca ctaaaacgaa 1140
agtttgccgc atgttcacaa agtgctgcgg aggacgttgt tgatcgtgct gcaaacccgt 1200
gtagaacgtc tattaataaa aataaagaat gcacatacag aaaacgaaaa atagatgagc 1260
tagaagacag cggatatggc aatactgaag tggaaactca gcagatggta caacaggtag 1320
aaagtcaaaa tggcgacaca aacttaaatg acttagaatc tagtggggtg ggggatgatt 1380
cagaagtaag ctgtgagaca aatgtagata gctgtgaaaa tgttacgttg caggaaatta 1440
gtaatgttct acatagtagt aatacaaaag caaatatatt atataaattt aaagaggcct 1500
atggaataag ttttatggaa ttagtaagac catttaaaag tgataaaaca agctgtacag 1560
attggtgtat aacaggatat ggaattagtc catcagtagc agaaagttta aaagtattaa 1620
ttaaacagca tagtttgtat actcatttac aatgtttaac ttgcgataga ggaataataa 1680
tattattgtt aattagattt aggtgtagca aaaacaggtt aacagtagca aaactaatga 1740
gtaatttatt atcaatacct gaaacatgta tggttataga gccaccaaaa ttacggagcc 1800
aaacatgtgc attgtattgg tttagaacag caatgtcaaa cattagtgat gtacaaggta 1860
caacacctga atggatagat agactaactg ttttacaaca tagctttaat gataatatat 1920
ttgatttaag tgaaatggta cagtgggcat atgataacga gttaacggac gatagtgaca 1980
ttgcatatta ttatgcacaa cttgcagatt caaatagtaa tgctgctgca tttttaaaaa 2040
gtaactcaca agcaaaaata gtaaaggact gtggaataat gtgtagacat tataaaaaag 2100
cagaaaaacg taaaatgtca ataggacaat ggatacaaag tagatgtgaa aaaacaaatg 2160
atggaggaaa ttggagacca atagtacagt tgttaagata tcaaaacatt gaatttacag 2220
catttttagg tgcatttaaa aagtttttaa aaggtatacc aaaaaaaagc tgtatgctaa 2280
tttgtggacc agcaaataca ggaaagtcat attttggaat gagtttaata cagtttttaa 2340
aagggtgtgt tatatcatgt gtaaattcta aaagtcactt ttggttgcag ccattatcag 2400
atgcaaaaat aggaatgata gatgatgtaa cgccaataag ttggacatat atagatgatt 2460
acatgagaaa tgcgttagat ggaaatgaaa tttcaataga tgtgaaacat agggcattag 2520
tgcaattaaa atgtccacca ctgcttctta cctcaaatac aaatgcaggc acagactcta 2580
gatggccata tttacatagt agattaacag tatttgaatt taaaaatcca ttcccatttg 2640
atgaaaatgg taacccagtg tatgcaataa atgatgaaaa ttggaaatcc tttttctcaa 2700
ggacgtggtg caaattagat ttaatagagg aagaggacaa ggaaaaccat ggaggaaata 2760
tcagcacgtt taaatgcagt gcaggagaaa atactagatc tttacgaagc tgataaaact 2820
gatttaccat cacaaattga acattggaaa ctgatacgca tggagtgtgc tttattgtat 2880
acagccaaac aaatgggatt ttcacattta tgccaccagg tggtgccttc tttgttagca 2940
tcaaagacca aagcatttca agtaattgaa ctacaaatgg cattagagac attaagtaaa 3000
tcacagtata gtacaagcca atggacattg caacaaacaa gcttagaggt gtggctttgt 3060
gaaccaccaa aatgttttaa aaaacaagga gaaacagtaa ctgtgcaata tgacaatgac 3120
aaaaaaaata caatggatta tacaaactgg ggtgaaatat atattataga ggaagataca 3180
tgtactatgg ttacagggaa agtagattat ataggtatgt attatataca taactgtgaa 3240
aaggtatatt ttaaatattt taaagaggat gctgcaaagt attctaaaac acaaatgtgg 3300
gaagtacatg tgggtggtca ggtaattgtt tgtcctacgt ctatatctag caaccaaata 3360
tccactactg aaactgctga catacagaca gacaacgata accgaccacc acaagcagcg 3420
gccaaacgac gacgacctgc agacaccaca gacaccgccc agccccttac aaagctgttc 3480
tgtgcagacc ccgccttgga caatagaaca gcacgtactg caactaactg cacaaacaag 3540
cagcggactg tgtgtagttc taacgttgca cctatagtgc atttaaaagg tgaatcaaat 3600
agtttaaaat gtttaagata cagattaaaa ccttataaag agttgtatag ttctatgtca 3660
tccacctggc attggaccag tgacaacaaa aatagtaaaa atggaattgt aactgtaaca 3720
tttgtaactg aacagcaaca acaaatgttt ttaggtaccg taaaaatacc acctactgtg 3780
caaataagta ctggatttat gacattataa gtgtacatca caagccaata tgtgctgcta 3840
attgtatata accatgatat ttgtttttgt attatgtttt atattgtttt tatgcttatc 3900
cttattatta cgtcctttaa tactttccat ttctacctat gcttggttgc tggtgttggt 3960
attgctgctt tgggtgtttg tgggatctcc tttaaaaatt tttttttgct atttgttgtt 4020
tttatattta ccaatgatgt gtattaattt tcatgcacag catatgacac aacaagagta 4080
atgtatatac atgtatatat tgtttgtata tatgtgcaca tggtggtgtt ttaacattgt 4140
tgttgttatt ttagtttttt tttttttgta ttactaataa atacctttat attttagcag 4200
tgtattatta tgagacacaa acgatctaca aggcgcaagc gtgcatctgc aacacaacta 4260
taccaaacat gcaaggccac aggcacctgc ccacccgatg ttattcctaa agtggaagga 4320
agtaccatag cagatcaaat tcttaaatat ggcagtttag gggttttttt tggtggttta 4380
ggtattggca caggctctgg ttcaggtgga aggactggct atgtacctat tggtactgac 4440
ccacctacag ctgcaatccc cttgcagcct atacgtcctc cggttactgt agacactgtt 4500
ggacctttag actcgtctat agtgtcatta atagaagaaa caagttttat agaggcaggt 4560
gcaccagccc catctattcc tacaccatca ggttttgatg ttactacatc tgcagatact 4620
acacctgcaa ttattaatgt ttcatctgtt ggggagtcat ctattcaaac tatttctaca 4680
catttaaatc ccacatttac tgaaccatct gtactacacc ctccagcgcc tgcagaagcc 4740
tctggacatt ttatattttc ttcccctact gttagcacac aaagttatga aaacatacca 4800
atggatacct ttgttgtttc cacagacagt agtaatgtaa catcaagcac gcccattcca 4860
gggtctcgcc ctgtggcacg ccttggttta tatagtcgca atacccaaca ggttaaggtt 4920
gttgaccctg cttttttaac atcgcctcat aaacttataa catatgataa tcctgcattt 4980
gaaagctttg accctgaaga cacattacaa tttcaacata gtgatatatc acctgctcct 5040
gatcctgact ttctagatat tattgcatta cataggcctg ctattacatc tcgtagacat 5100
actgtgcgtt ttagtagagt aggtcaaaaa gccacactta aaactcgcag tggtaaacaa 5160
attggagcta gaatacatta ttatcaggat ttaagtccta ttgtgccttt agaccacacc 5220
gtgccaaatg aacaatatga attacagcct ttacatgata cttctacatc gtcttatagt 5280
attaatgatg gtttgtatga tgtttatgct gacgatgtgg ataatgtaca caccccaatg 5340
caacactcat acagtacgtt tgcaacaaca cgtaccagca atgtgtctat acctttaaat 5400
acaggatttg atactcctgt tatgtctggc cctgatatac cttccccttt atttcccaca 5460
tctagcccat ttgttcctat ttcgcctttt tttccttttg acaccattgt tgtagacggt 5520
gctgactttg ttttacatcc tagttatttt attttacgtc gcaggcgtaa acgttttcca 5580
tattttttta cagatgtccg tgtggcggcc tagtgaggcc acagtgtacc tgcctcctgt 5640
acctgtatct aaagttgtca gcactgatga atatgtgtct cgcacaagca tttattatta 5700
tgctggtagt tccagacttc ttgctgttgg ccatccatat ttttctatta aaaatcctac 5760
taacgctaaa aaattattgg tacccaaagt atcaggcttg caatataggg tttttagggt 5820
ccgtttacca gatcctaata aatttggatt tcctgacacc tccttttata accctgatac 5880
acaacgatta gtatgggcat gtgtaggcct tgaaataggt agagggcagc cattaggcgt 5940
tggcataagt ggtcatcctt tattaaacaa atttgatgac actgaaaccg gtaacaagta 6000
tcctggacaa ccgggtgctg ataataggga atgtttatcc atggattata aacaaacaca 6060
gttatgttta cttggatgta agcctccaac aggggaacat tggggtaaag gtgttgcttg 6120
tactaatgca gcacctgcca atgattgtcc acctttagaa cttataaata ctattattga 6180
ggatggtgat atggtggaca caggatttgg ttgcatggat tttaaaacat tgcaggctaa 6240
taaaagtgat gttcctattg atatttgtgg cagtacatgc aaatatccag attatttaaa 6300
aatgactagt gagccttatg gtgatagttt atttttcttt cttcgacgtg aacaaatgtt 6360
tgtaagacac ttttttaata gggctggtac attaggagag gctgttcccg atgacctgta 6420
cattaaaggt tcaggaacta ctgcctctat tcaaagcagt gctttttttc ccactcctag 6480
tggatcaatg gttacttccg aatctcagtt atttaataag ccatattggc tacaacgtgc 6540
acaaggtcat aataatggta tttgttgggg caatcaggta tttgttactg tggtagatac 6600
cactcgcagt actaatatga ctttatgcac acaagtaact agtgacagta catataaaaa 6660
tgaaaatttt aaagaatata taagacatgt tgaagaatat gatctacagt ttgtttttca 6720
actatgcaaa gttaccttaa ctgcagaagt tatgacatat attcatgcta tgaatccaga 6780
tattttagaa gattggcaat ttggtttaac acctcctcca tctgctagtt tacaggatac 6840
ctataggttt gttacctctc aggctattac gtgtcaaaaa acagtacctc caaaggaaaa 6900
ggaagacccc ttaggtaaat atacattttg ggaagtggat ttaaaggaaa aattttcagc 6960
agatttagat cagtttcctt tgggacgcaa gtttttatta caggcaggtc ttaaagcaaa 7020
acctaaactt aaacgtgcag cccccacatc cacccgcaca tcgtctgcaa aacgcaaaaa 7080
ggttaaaaaa taacactttg tgtaattgtg ttatgttgtt gttttgttct gtctatgtac 7140
tttgtgttgt tgtgttgtgt tgttgtttgt tttttgtgta tgtgttacaa tgtatgttat 7200
gttgtatgtt actgtgtttg ttttatgtgt acttgtttgt gtgcatgttc tatgtacttg 7260
tcagtttcct gtttgtgtat atgttaataa aacattgtgt gtatttgtta aactatttgt 7320
atgtatgtta tgtatatggg tgtacctata tgagtaagga gttgtattgc ttgccctacc 7380
ctgcattgca atgtacctac ctttatttcc ctatatttgt agtacctaca tgtttagtat 7440
tgctttacct tttgacatac tagtgtccat attgtacaat ttcctccatt ttgtatgcct 7500
aaccgttttc ggttacttgg catacatacc ctatgacatt ggcagaacag ttaatccttt 7560
tctttcctgc actgtgtttg tctgtacttg ctgcattggc atacataccc tatgacattg 7620
gcagaacagt taatcctttt ctttcctgca ctgtgtttgt ctgtacttgc tgcattgact 7680
catatataca tgcagtgcaa ttgcaaaata cttaattgta ctaatagttt acacatgctt 7740
ttaggcacat atttttactt tactttcaaa ccttaagtgc agttttggct tacacaattg 7800
ctttgtatgc caaactatgc cttgtaaaag tgagtcacta cctgtttatt accaggtgtg 7860
gactaaccgt tttaggtcat attggtcatt tataatcttt tatataata 7909
<210> 5
<211> 7879
<212> DNA
<213> Artificial Sequence
<220>
<223> Artificial Synthesis
<400> 5
ccctataaaa aaaacaggga gtgaccgaaa acggtcgtac cgaaaacggt tgccataaaa 60
gcagaagtgc acaaaaaagc agaagtggac agacattgta aggtgcggta tgtttcagga 120
cccagctgaa cgaccttaca aactgcatga tttgtgcaac gaggtagaag aaagcatcca 180
tgaaatttgt ttgaattgtg tatactgcaa acaagaatta cagcggagtg aggtatatga 240
ctttgcatgc tatgatttgt gtatagtata tagagaaggc cagccatatg gagtatgcat 300
gaaatgttta aaattttatt caaaaataag tgaatataga tggtatagat atagtgtgta 360
tggagaaacg ttagaaaaac aatgcaacaa acagttatgt catttattaa ttaggtgtat 420
tacatgtcaa aaaccgctgt gtccagttga aaagcaaaga catttagaag aaaaaaaacg 480
attccataac atcggtggac ggtggacagg tcggtgtatg tcctgttgga aaccaacacg 540
tagagaaacc gaggtgtaat catgcatgga gaaataacta cattgcaaga ctatgtttta 600
gatttggaac ccgaggcaac tgacctatac tgttatgagc aattgtgtga cagctcagag 660
gaggaggaag atactattga cggtccagct ggacaagcaa aaccagacac ctccaattat 720
aatattgtaa cgtcctgttg taaatgtgag gcgacactac gtctgtgtgt acagagcaca 780
cacattgaca tacgtaaatt ggaagattta ttaatgggca catttggaat agtgtgcccc 840
ggctgttcac agagagcata atctacaatg gctgatcctg caggtacaga tgaaggggag 900
gggacgggat gtaatggatg gttttttgta gaagcagtag ttagtagacg tacgggggat 960
ccagtgtcag aggacgaaaa tgaagatgac tgtgacaggg gggaggatat ggtggacttt 1020
ataaatgata cagatatatt aaacatacag gcagaaacag agacagcaca agcattattt 1080
catgcacagg aggagcaaac acacaaagag gctgtacagg tcctaaaacg aaagtatgct 1140
agtagtccac ttagcagcgt gagcttatgt gttaataata acataagtcc acgtttaaaa 1200
gctatttgca ttgaaaataa aaatacagca gcaaagcgac gattatttga actaccagac 1260
agcggttatg gcaattctga agtggaaata cagcagatac aacaggtaga ggggcatgat 1320
acagttgaac aatgtagtat gggcagtggg gatagtataa cctctagtag cgatgaaaga 1380
catgatgaga ctccaacgcg agacataata caaatactaa aatgtagtaa tgcaaacgca 1440
gctatgttgg ctaaatttaa agaactattt ggtattagtt ttacagaact tattagacca 1500
tttaagagtg ataaatccac atgtacagat tggtgtgtgg ccgcatttgg aatagcccca 1560
agtgtggcgg aaagtttaaa aacattaatt aaaccatatt gtttatatat acatatacaa 1620
tgtttatcgt gttcatgggg tatggtaatt ctagcattat tacgatttaa atgtgcaaaa 1680
aacagaacaa caattgaaaa actattatca aaattgctat gtatttcagc tgcaagtatg 1740
ctaatacaac caccaaaatt acgtagtacc ccagctgcgt tatattggtt taaaacagca 1800
atgtcaaata ttagtgaggt tgatggagaa acaccagaat ggattcaaag acaaacagta 1860
ttacagcata gttttaatga tgcaatattt gacctatctg aaatggtaca atgggcatat 1920
gacaatgatt ttatagatga tagtgatata gcatataaat atgcacaatt ggcagaaact 1980
aatagtaatg catgtgcttt tttaaaaagt aattcgcaag ctaaaattgt aaaagattgt 2040
gcaacaatgt gtagacatta taaacgagct gaaaaaagag aaatgacaat gtcacagtgg 2100
attaaaaggc gatgtgaaaa ggtggacgat gacggtgact ggagggacat agtacgattt 2160
ttaagatatc aacaagtaga ttttgtggca tttttatctg cactaaaaaa ttttttacat 2220
ggtgtgccta aaaaaaattg catacttata tatggagcac caaacacagg taaatcatta 2280
tttggaatga gtctaatgca tttcttacaa ggagctatta tatcctatgt aaattctaaa 2340
agccattttt ggttgcagcc attatatgat gccaaaatag ctatgttaga tgatgctaca 2400
tcgccatgtt gggcatatat agaccaatat ttaagaaatg cactagatgg aaatcctatt 2460
tcattagatg taaagcataa agcattagtg caattaaaat gcccaccttt acttattaca 2520
tcaaatataa atgcaggcaa agatgacagg tggccatact tacatagcag ggtagtggtc 2580
tttacatttc acaatgaatt cccatttgat aaaaatggaa acccagtgta tgggcttaat 2640
gataaaaact ggaaatcctt tttctcaagg acgtggtgca gattaaattt gcacgaggaa 2700
gaggacaaag aaaatgatgg agacgctttc ccagcgttta agtgtgtgtc aggacaaaat 2760
actagaacat tacgagactg atagcacatg tttgtctgat cacatacagt attggaaact 2820
gattcgtctt gaatgtgcag tattttataa agcaagagaa atgggaatta aaactcttaa 2880
ccaccaagtg gttccaacgc aggccatttc aaaagccaaa gcaatgcaag caattgaact 2940
gcaattaatg ttagagacat taaatacaac tgagtatagc acagaaacat ggacactgca 3000
agaaacaagt attgaattat atacaacagt tccacaagga tgttttaaaa aacatggggt 3060
tacagtggaa gtacaatttg atggtgataa acaaaatact atgcattata ctaattggac 3120
acatatatat atattagagg acagtatatg tactgttgta aagggactgg taaattataa 3180
aggtatttat tatgtgcatc agggtgtaga aacatattat gttactttta gggaagaggc 3240
taaaaagtat ggaaaaaaaa atatatggga agtgcatgtg ggtggtcagg taattgtttg 3300
tcctgaatct gtatttagca gcacagaact atccactgct gaaattgcta cacagctaca 3360
cgcctacaac accaccgaga cccataccaa agcctgctcc gtgggcacca cagaaaccca 3420
gaagacaaat cacaaacgac ttcgaggggg taccgagctc ccctacaacc ccaccaagcg 3480
agtgcgactc agtgccgtgg acagtgttga cagaggggtc tactctacat ctgactgcac 3540
aaacaaagac cggtgtggta gttgtagtac aactacacct atagtacatt taaaaggtga 3600
tgcaaataca ttaaagtgtt taagatatag attgggtaaa tataaagcat tgtatcaaga 3660
tgcttcatct acatggagat ggacatgtac aaacgataaa aaacaaatag caattgtaac 3720
attaacttac acaacagaat atcaaaggga taaattttta actacagtaa aaatacctaa 3780
cacagttaca gtgtctaaag gatatatgtc tatatgatag accttacagc ttccagtact 3840
gtgttgctgt gctttttgtt gtgcttttgt gtgcttttgt gcttgtgtct gcttgtacgt 3900
tcgctattgc tatctgtgtc attatactca gcattaatat tactggtttt aatactgtgg 3960
gttactgtag caacaccact acgttgcttt tgttgttttc tttgcttttt gtatatacct 4020
atgggaatga ttaacgctca tgcacaatat ttggcagtac agtaattgta tacaaacatt 4080
gtgtttggta ctgtgtaaca tgtgtgtatg gtggttttat tttttgttgt tcattgtata 4140
ttttgttttt ttactgtttt taaacatttt tatttctgtg tttttaataa attgatcaca 4200
tggtataacc atgcgacaca aaaggtctac aaaacgtgtt aaacgtgcat ctgcaacaca 4260
actatatcgt acttgcaaag ctgcaggaac ttgtccacca gatgttatac ctaaggttga 4320
gggtaatact gttgctgatc aaattttaaa atatggcagc atggctgtgt tttttggggg 4380
gttaggaatt ggttctggat ctggcacagg tggaagatct ggatatgttc cactgggtac 4440
aacacctcca acggctgcca caaacattcc tatacgaccc cctgtaactg tggaaagtat 4500
accattagac acaattggcc ctttagattc ttctatagtg tcattagtag aggaaactag 4560
ttttattgag tctggtgccc ctgttgttac accaagggtc ccacctacaa caggttttac 4620
aataaccaca tctacagata ccacacctgc tattttagat gtgacatcca taagtacaca 4680
tgataatcct actttcactg atccttctgt tttacaccca cccacgcctg cagaaacttc 4740
aggtcatttt gtactttcat catcttctat tagtacacat aattatgaag aaatccctat 4800
ggatactttt attgtttcca cagacagcaa taatataact aatagcacgc ctattccagg 4860
gtctcgccct acgacacgcc taggattata tagtaaaggt acccagcagg ttaaggttgt 4920
tgaccctgcc tttatgactt ctcctgcaaa acttattaca tatgataatc ctgcatatga 4980
aggccttaac cctgatacaa ccttacaatt tgagcatgag gatattagct tagctccgga 5040
tcctgacttt atggacatta tagctttaca taggcctgca ctaacatcta ggaaaggcac 5100
tattagatat agtagagtag gtaataaacg tactatgcat acacgaagtg gaaaagctat 5160
aggggcacgg gtacattatt atcaggattt aagtagtatt actgaagata tagaattaca 5220
acccttacaa catgtaccat cctctttacc acataccact gtttcaacat cattaaatga 5280
tggtatgttt gatatttatg ctcctataga tactgaggaa gatattatat tttcagcatc 5340
ttctaacaat actttatata ctacatctaa cactgcatat gttcctagca atactactat 5400
accattaagt agtggctatg atattcctat aacagcaggg ccagacattg tatttaactc 5460
taatactatt actaacactg tactaccggt acccacaggt cctatatatt ctattattgc 5520
agatgggggt gacttttatt tacaccctag ttattattta ttaaaacgac gtcgtaaacg 5580
tatcccatat ttttttgcag atgtctctgt ggcggtctaa cgaagccact gtctacctgc 5640
ctccagtgtc agtgtctaag gttgttagca ctgatgaata tgtaacacgc acaaacatct 5700
actatcatgc aggcagttct aggctattag ctgtgggtca cccatactat gctattaaaa 5760
aacaagattc taataaaata gcagtaccca aggtatctgg tttgcaatac agagtattta 5820
gagtaaaatt accagatcct aataagtttg gatttccaga cacatcattt tatgatcctg 5880
cctcccagcg tttggtttgg gcctgtacag gagttgaagt aggtcgtggt cagccattgg 5940
gtgtaggtat tagtggtcat cctttattaa ataaattgga tgatactgaa aattctaata 6000
aatatgttgg taactctggt acagataaca gggaatgcat ttctatggat tataaacaaa 6060
cacaattgtg tttaataggt tgtaggcctc ctataggtga acattgggga aaaggcacac 6120
cttgtaatgc taaccaggta aaagcaggag aatgtcctcc tttggagtta ctaaacactg 6180
tactacaaga cggggacatg gtagacacag gatttggtgc aatggatttt actacattac 6240
aagctaataa aagtgatgtt cccctagata tatgcagttc catttgcaaa tatcctgatt 6300
atctaaaaat ggtttctgag ccatatggag atatgttatt tttttattta cgtagggagc 6360
aaatgtttgt tagacattta tttaataggg ctggaactgt aggtgaaaca gtacctgcag 6420
acctatatat taagggtacc actggcacat tgcctagtac tagttatttt cctactccta 6480
gtggctctat ggtaacctcc gatgcacaaa tatttaataa accatattgg ttgcaacgtg 6540
cacaaggcca taataatggt atttgttgga gtaaccaatt gtttgttact gtagttgata 6600
caacccgtag tacaaatatg tctgtgtgtt ctgctgtgtc ttctagtgac agtacatata 6660
aaaatgacaa ttttaaggaa tatttaaggc atggtgaaga atatgattta cagtttattt 6720
ttcagttatg taaaataaca ctaacagcag atgttatgac atatattcat agtatgaacc 6780
cgtccatttt agaggattgg aattttggcc ttacaccacc gccttctggt accttagagg 6840
acacatatcg ctatgtaaca tcacaggctg taacttgtca aaaacccagt gcaccaaaac 6900
ctaaagatga tccattaaaa aattatactt tttgggaggt tgatttaaag gaaaagtttt 6960
ctgcagactt agatcaattt ccgttgggcc gtaaattttt gttacaagca ggactaaagg 7020
ccaggcctaa ttttagatta ggcaagcgtg cagctccagc atctacatct aaaaaatctt 7080
ctactaaacg tagaaaagta aaaagttaat gtgtaaatgt gtatgcatgt atactgtgtg 7140
ttatgtgttg tagtgcttgt atatatatta tgtgttgtgg tgcctgtttg tgttgtacat 7200
ggcgtgtaaa tgtgtgtata atattgtgca atgtgttgta cgtgggtgtt ttttgtatgt 7260
atgttgttgt atgtatgtca gtacgcaata aaagtgatgt gtgtgtttat aattaacact 7320
gtattgttgt atgactatgg gtgcacccat atgacttaca taattacagt acacgctata 7380
tgttgtatat aacaattcta cctccatttt gtgtgttagt gtcctttaca ttacctttca 7440
accgatttcg gttgctgttg gcaagcttta tatgtttttt acaaaaacat tcctacctca 7500
gcagaacact taatccttgt gttcctgata tatattgttt gccaacttta tattggcttt 7560
tgccaatctt taaacttgat tcatcttgca gtattagtca tttttcatac ttgtggtcca 7620
cccacacttg taacacttgt aacagtgctt ttaggcacat attttttgca tttctaaagg 7680
gctttaattg cacacttggc tttacatatt atgtgtgttt gccaacacca ccctacacat 7740
cctgccaact ttaagttaaa acatgcatgt aaaacattac tcactgtatt acacattgtt 7800
atatgcacac aggtgtgtcc aaccgatttg gattacagtt ttataagcat ttctttttat 7860
tatagttagt aacaattat 7879
<210> 6
<211> 7833
<212> DNA
<213> Artificial Sequence
<220>
<223> Artificial Synthesis
<400> 6
cttataacat tttataagta tcttgtttaa aaaaagggag taaccgaaaa cggtcaggac 60
cgaaatcggt ggatataaaa cgcagtcaca gtttctgtcc ataccgatgg cgcgatttca 120
caatcctgca gaacggccat acaaattgcc agacctgtgc acaacgctgg acaccacctt 180
gcaggacatt acaatagcct gtgtctattg cagacgacca ctacagcaaa ccgaggtata 240
tgaatttgca tttagtgatt tatatgtagt atatagggac ggggaaccac tagctgcatg 300
ccaatcatgt ataaaatttt atgctaaaat acgggagcta cgatattact cggactcggt 360
gtatgcaact acattagaaa atataactaa tacaaagtta tataatttat taataaggtg 420
catgtgttgt ctgaaaccgc tgtgtccagc agaaaaatta agacacctaa atagcaaacg 480
aagatttcat aaaatagcag gaagctatac aggacagtgt cgacggtgct ggaccacaaa 540
acgggaggac cgcagactaa cacgaagaga aacccaagta taacatcaga tatgcgtgga 600
ccaaagccca ccttgcagga aattgtatta gatttatgtc cttacaatga aatacagccg 660
gttgaccttg tatgtcacga gcaattagga gagtcagagg atgaaataga tgaacccgac 720
catgcagtta atcaccaaca tcaactacta gccagacggg atgaaccaca gcgtcacaca 780
atacagtgtt cgtgttgtaa gtgtaacaac acactgcagc tggtagtaga agcctcacgg 840
gatactctgc gacaactaca gcagctgttt atggactcac taggatttgt gtgtccgtgg 900
tgtgcaactg caaaccagta acctgctatg gccaatcgtg aaggtacaga cggggatggg 960
tcgggatgta acggatggtt tctagtacag gcaatagtag ataaacaaac aggcgacaca 1020
gtgtcggagg atgaggatga aaatgcaaca gatacaggtt cagacctggc agactttatt 1080
gatgattcca cagatatttg tgtacaggca gagcgtgaga cagcacaggt acttttacat 1140
atgcaagagg cccaaaggga tgcacaagca gtgcgtgcct taaaacgaaa gtatacagac 1200
agcagtggcg acactagacc gtatggaaaa aaagtaggca ggaataccag gggaacacta 1260
caggaaattt cattaaatgt aagcagtacg caggcaacac aaacggtgta ttccgtgcca 1320
gacagcggat atggcaatat ggaagtggaa acagctgaag tggaggaggt aactgtagca 1380
actaatacaa atggggatgc tgaaggggaa catggcggca gtgtacggga ggagtgcagt 1440
agtgtggata gtgctataga tagtgaaaac caggatccca aatctccaac tgcacaaatt 1500
aaattattgt tacaatccaa taacaaaaag gctgcaatgc taacacaatt taaagaaaca 1560
tatggactat cctttactga cctggtacgt acgtttaaaa gtgataaaac aacatgtaca 1620
gactgggtgg cagccatatt tggagtacat ccaactattg cagaaggatt taaaacatta 1680
atcaacaaat atgccttata tacacatata caaagcttag acacaaaaca aggagtacta 1740
attttaatgc taataagata tacatgtgga aaaaataggg ttactgtagg aaagggatta 1800
agtacattgt tacatgttcc agaaagttgt atgcttctgg agcctcctaa actgcgcagc 1860
cctgtagcag cactatattg gtatcgcaca ggtatatcca atattagtgt ggtaacaggg 1920
gatacgccag aatggataca acgattaact gttatacaac atggaataga tgatagtgta 1980
tttgacctat cggacatggt acaatgggca tttgacaatg aatatactga tgaaagtgac 2040
atagcattta attatgcaat gttagcagat tgtaacagta atgctgcagc ctttttaaaa 2100
agtaactgcc aggcaaaata tgtaaaagat tgtgcaacaa tgtgtaaaca ttacaagcga 2160
gcacaaaaaa ggcaaatgtc catgtctcaa tggataaaat ttaggtgtag taaatgtgat 2220
gaaggcgggg actggagacc catagtacaa ttcttaagat atcaaggaat agaatttata 2280
tcctttttat gtgcattaaa ggaattttta aagggtactc ccaaaaaaaa ctgtatagtt 2340
atatatggac ctgcgaatac aggaaagtca catttttgta tgagccttat gcatttttta 2400
cagggcacag ttatttcata tgtaaactcc accagccact tttggctaga accacttgca 2460
gatgcaaaac tagcaatgtt agatgatgca accggtacct gctggtcata tttcgataat 2520
tatatgagaa atgcattaga tgggtatgca ataagtttag ataggaaata taaaagttta 2580
ctacaaatga aatgtccacc attattaata acctccaata ccaatcctgt ggaagacgat 2640
aggtggccat atttacgtag taggctaaca gtgtttaaat ttcctaatgc atttccattt 2700
gaccaaaaca ggaatccagt gtacacaatc aatgataaaa actggaaatg tttttttgaa 2760
aagacttggt gcagattaga cttgcagcag gacgaggatg aaggagacaa tgatgaaaac 2820
actttcacaa cgtttaaatg tgttacagga caaaatacta gaatactatg aacaagacag 2880
taaatcaata tatgatcaaa ttaattattg gaaatgtgtg cgaatggaaa atgcaatatt 2940
ttatgcagca cgagaacgtg gcatgcatac tattgaccac caggtggtgc caaccataaa 3000
catttcaaaa tgtaaagcat atcaagctat tgaactgcag atggcactag aaagtgttgc 3060
acaaactgaa tacaatacag aggagtggac attaaaagac actagtaatg aactgtggca 3120
tacacagcca aaacaatgtt ttaaaaaaca aggaactaca gtggaggtgt ggtatgatgg 3180
ggacaaatgt aatgctatga actatgtatt atggggtgct atatattata aaaataatat 3240
agacatatgg tgtaaaacag aagggtgtgt ggactattgg ggtatatatt atatgaacga 3300
gcacctaaaa gtatactatg aagtgtttat tcaagatgcg gaaaggtatg ggactagtgg 3360
caaatgggaa gtgcattata atggcaacat aattcattgt cctgactcta tgtgcagtac 3420
cagtgacgga tcggtaccca ctactgaact tactaccgaa ttatcaaaca ccaccgcgac 3480
ccattccacc gcaacaaccc catgcaccca aaaaacaatc ccgccgccgt ctcgaaagcg 3540
acctcgacag tgtgcagtca cagagcccac tgagcccgac ggagtgtccc tggaccatct 3600
taacaaccca ctccacagta acagtacagg ccacaacaca agacggtacc tcagttgtgg 3660
taacactacg cctataatac atttaaaagg tgacaaaaat ggtttaaaat gtttaagata 3720
tagactacaa aaatatgaca cattgtttga aaatatttca tgtacctggc attggatacg 3780
gggtaaggga accaaaaacg ctggcatatt aactgttaca tatgccacag agtcacaacg 3840
ccaaaaattt ttggacactg ttaaaatacc ttctagtgta catgtttcat tgggttacat 3900
gacattgtaa agtatactat ggatattgtg tatgtatatt gtatacatac tacatagatg 3960
atattattgg tatttttggt gtggtttggt gtgtgtatat atatatgttg caatgtcccg 4020
cttttgccgt ctgtgcatgt gtgtgcgtat gtgtggataa ttgtgtttgt gtttattctt 4080
atacgtacca caccattgga ggtgtttttt gtatatttac tattttttgt attgcccatg 4140
tggttgttgc atagactggc aatggatatg atatagtact gtatatgtat gtgcattgtg 4200
cataactact gtacatagct ttttatattt ttttttgtta ctaataaaca tggtttccca 4260
ccgtgctgcc aggcgtaagc gtgcatctgc aactgaccta tatagaacct gtaaacaatc 4320
gggtacctgt ccaccagacg ttgttgataa agttgagggt actacacttg ctgacaaaat 4380
tttacagtgg actagtttag gtatattttt gggtgggtta ggcataggca caggtactgg 4440
tactggggga cgcacaggat atatacccct ggggggtagg cctaatactg ttgtagatgt 4500
gtctcctgca cgtccacctg tagttattga acctgttggt ccttctgagc catctattgt 4560
gcaattggtg gaggactcaa gtgttataac ctctggaaca ccagtaccaa catttacagg 4620
cacctctgga tttgaaatta cttcttcttc tactactacg cctgcggtat tggatattac 4680
accctcctct gggtctgtac aaataacctc tactagttat actaaccctg cctttacgga 4740
tccttcctta attgaggttc cccaaacagg tgaaacctcg ggtaatatat ttgtcagtac 4800
ccctacatca ggtacacatg gctatgagga aatacctatg gaagtgtttg ccacacatgg 4860
cacaggtacc gaacctatta gcagcacacc tacacctgga atcagtcgtg tggcaggacc 4920
acgtttatat agtagagcac atcagcaggt tcgtgttagt aattttgatt ttgtaactca 4980
cccttcatca tttgtaacat ttgataatcc tgcttttgag cctgttgata ctacattaac 5040
atatgaagct gctgacatag ctccagatcc ggattttctg gacattgttc gtttacatag 5100
gcctgcctta acctcgcgta aaggaacagt aaggtttagt aggcttggca aaaaggctac 5160
catggttacc cggcgtggca cacaaattgg agcgcaagta cattattacc atgacattag 5220
tagtattgct cctgctgaaa gcattgaatt acagccccta gttcacgctg agccctctga 5280
tgcttcagat gcattatttg atatatatgc tgatgtggac aataacacat atttagatac 5340
tgcatttaat aatacaaggg attcgggcac tacatataac acaggctcac taccttctgt 5400
ggcttcttca gcatctacta aatatgccaa tacaactatt ccttttagta cctcatggaa 5460
tatgcctgta aatactggtc ctgatattgc tttaccaagt actactccac agttgccatt 5520
ggtgccttct ggaccaatag acacaacata tgcaataacc attcagggtt ccaattatta 5580
tttgttgcca ttattgtatt ttttcctaaa aaaacgtaaa cgtattccct attttttttc 5640
agatggctat gtggcggtct agtgacagca tggtgtattt gcctccacct tctgtggcga 5700
aggttgtcaa tactgatgat tatgttacac gcacaggcat atattattat gctggcagct 5760
ctagattatt aacagtagga catccatatt ttaaagtggg tatgaatggt ggtcgcaagc 5820
aggacattcc aaaggtgtct gcatatcaat atagggtatt tcgcgtgaca ttgcccgatc 5880
ctaataaatt cagtattcca gatgcatcct tatataatcc agaaacacaa cgtttagtat 5940
gggcttgtgt aggggtggag gtgggcaggg gccagccatt gggtgttggt attagtggac 6000
acccattata taatagacag gatgatactg aaaactcacc attttcatca accaccaata 6060
aggacagtag ggataatgtg tctgtggatt ataaacagac acagttgtgc attataggct 6120
gtgttcccgc cattggggag cactggggta agggaaaggc atgcaagccc aataatgtat 6180
ctacggggga ctgtcctcct ttggaactag taaacacccc tattgaggat ggtgatatga 6240
ttgatactgg ctatggagct atggactttg gtgcattgca ggaaaccaaa agtgaggtgc 6300
ctttagatat ttgtcaatcc atttgtaaat atcctgatta tttgcaaatg tctgcagatg 6360
tgtatgggga cagtatgttc ttctgtttac gtagggaaca actgtttgca agacattttt 6420
ggaatcgtgg tggtatggtg ggtgacgcca ttcctgccca attgtatatt aagggcacag 6480
atatacgtgc aaaccccggt agttctgtat actgcccctc tcccagcggt tccatggtaa 6540
cctctgattc ccagttattt aataagcctt attggctaca taaggcccag ggccacaaca 6600
atggtatatg ttggcataat caattatttc ttactgttgt ggacactacc cgtagtacca 6660
actttacatt atctacctct atagagtctt ccataccttc tacatatgat ccttctaagt 6720
ttaaggaata taccaggcac gtggaggagt atgatttaca atttatattt caactgtgta 6780
ctgtcacatt aacaactgat gttatgtctt atattcacac tatgaattcc tctatattgg 6840
acaattggaa ttttgctgta gctcctccac catctgccag tttggtagac acttacagat 6900
acctacagtc tgcagccatt acatgtcaaa aggatgctcc agcacctgaa aagaaagatc 6960
catatgacgg tctaaagttt tggaatgttg acttaaggga aaagtttagt ttggaacttg 7020
atcaattccc tttgggacgt aaatttttgt tgcaggccag ggtccgcagg cgccctacta 7080
taggtccccg aaagcggcct gctgcatcca cttcctcgtc ctcagctact aaacacaaac 7140
gtaaacgtgt gtctaaataa tgcatgtgta tgccttgtta tgtgtgtgta tgttgtttgt 7200
ttccttatgt gttgagtgta tatgtgtatg tttgtaggta tgtgtgtata tgtttttgtt 7260
aataaagtat gtatgacagt ttcatgtgtg attgcacacc ctgtgactaa cagtgtattt 7320
gttttacata taataggtct gcaacatttc atacataatc tatatgccct accctaaggt 7380
gtgtttacta cctaatatgt aatttttaca ttgttgtatg cgtttctaca ttttatactt 7440
cgccattttg tggcgaccga agtcggtcgt gggttgagca ttttttttaa actagtggaa 7500
accacctttc tcagcaaaaa catgtcttta ccttaggttc accctgcata gttggcactg 7560
gtaacagttt tactggcgcg ccttattact catcatcctg tccaggtgca ctgcaacaat 7620
actttggcaa catccatatc tccaccctat gtaataaaac tgcttttagg catatatttt 7680
agctgttttt acttgcttaa ttaaatagtt ggcctgtata actacttttt gattcaggaa 7740
tgtgtcttac agtataagtt atacaagtga ctaatgtagc acacaatagt ttatgcaacc 7800
gaaataggtt gggcatacat acctatactt tta 7833
<210> 7
<211> 7858
<212> DNA
<213> Artificial Sequence
<220>
<223> Artificial Synthesis
<400> 7
aatactttta acaattatac tacataaaaa agggtgtaac cgaaaacggt tgcaaccaaa 60
aacggtgcat ataaaagctt tgtggaaaag tgcattacag gatggcgcgc tttgacgatc 120
caaagcaacg accctacaag ctaccagatt tgtgcacaga attgaataca tcactacaag 180
acgtatctat tgcctgtgta tattgcaaag caacattgga acgcacagag gtatatcaat 240
ttgcttttaa agatttatgt atagtgtata gagactgtat agcatatgct gcatgccata 300
aatgtataga cttttattcc agaattagag aattaagata ttattcaaac tctgtatatg 360
gagagacact ggaaaaaata actaatacag agttgtataa tttgttaata aggtgcctgc 420
ggtgccagaa accattgaac ccagcagaaa aacgtagaca ccttaaggac aaacgaagat 480
ttcacagcat agctggacag taccgagggc agtgtaatac atgttgtgac caggcacggc 540
aagaaagact tcgcagacgt agggaaacac aagtatagca ataagtatgc atggaccccg 600
ggaaacactg caagaaattg tattgcattt ggaacctcag aatgaattag atcctgttga 660
cctgttgtgt tacgagcaat taagcgagtc agaggaggaa aacgatgaag cagatggagt 720
tagtcatgca caactaccag cccgacgagc cgaaccacag cgtcacaaaa ttttgtgtgt 780
atgttgtaag tgtgacggca gaattgagct tacagtagag agctcggcag aggaccttag 840
aacactacag cagctgtttt tgagcacctt gtcctttgtg tgtccgtggt gtgcaactaa 900
ccaataatct acaatggcgg atccagaagg taccgacggg gagggaacgg ggtgtaatgg 960
ctggttcttt gtagaaacaa ttgtagagaa aaaaacaggg gatgtaatat cagatgatga 1020
ggatgaaact gcaacagata cagggtcgga tatggtagat tttattgaca cacaattatc 1080
catttgtgaa caggcagagc aagagacagc acaggcattg ttccatgcgc aggaagttca 1140
gaatgatgca caggtgttgc atcttttaaa acgaaagttt gcaggaggca gcaaggaaaa 1200
cagtccatta ggggagcagc taagtgtgga tacggatcta agtccacggt tacaagaaat 1260
ttcattaaat agtgggcaca aaaaagcaaa acgacggttg tttacaatat cagatagtgg 1320
ctatggctgt tctgaagtgg aagctgcaga gactcaggta actgtaaaca ctaatgcgga 1380
aaatggcggc agtgtacata gtacacaaag tagtggtggg gatagtagtg acaatgcaga 1440
aaatgtagat ccgcattgca gtattacaga actaaaggag ctattacaag caagtaacaa 1500
aaaggctgca atgctggcag tatttaaaga catatatggg ctgtcattta cggatttggt 1560
tagaaatttt aaaagtgata aaacaacatg tacagattgg gtaatggcta tatttggagt 1620
taatccaacg gtagcagaag gctttaaaac attaattaaa ccagcaacgt tatacgccca 1680
tatccaatgt ttagattgta aatggggagt attaatatta gctttattaa gatataaatg 1740
tggcaaaaat agactaactg ttgcaaaagg cttaagcaca ttgttgcacg tacctgaaac 1800
atgtatgtta attgaaccac caaaattgcg aagtagtgtt gcagcattat actggtatag 1860
aacaggtata tccaatatta gtgaagtaag tggagacaca cctgagtgga tacaaagact 1920
gacaattatt caacatggta ttgacgatag taattttgat ttgtcagaca tggtgcaatg 1980
ggcatttgat aatgacctta cagatgaaag tgatatggca tttcaatatg cccaattagc 2040
agactgcaac agtaatgcag ctgcattttt aaaaagtaac tgccaagcca aatatttaaa 2100
agattgtgct gtaatgtgta gacattataa aagagcacaa aaacgccaaa tgaatatgtc 2160
tcaatggatt aaatatagat gttccaaaat agatgaaggt ggggattgga gacccatagt 2220
acaattccta agatatcagg gagtagaatt tattagcttt ttaagggcac taaaggaatt 2280
tcttaaagga acaccaaaaa aaaattgtat actgttatat ggacctgcaa atacaggaaa 2340
atcgtatttt ggaatgagtt ttatacattt cctacaaggt gcaataatat catttgtaaa 2400
ttcaaacagc catttttggt tagaaccgtt agcagatact aaggtagcca tgttggatga 2460
tgccacacac acgtgttgga catattttga taattatatg agaaatgcat tagatggtaa 2520
tcctataagt atagacagaa agcataaacc attattacag ctaaaatgtc ctccaatcct 2580
attaacatcc aatattgatc cagcaaaaga taataaatgg ccatatttag aaagtagggt 2640
gacggtattt acatttccac atgcatttcc atttgataaa aatggtaatc cagtatatga 2700
aataaatgat aaaaattgga aatgtttttt tgaaaggaca tggtccagat tagatttgca 2760
cgaggacgat gaagatgcag acaccgaagg aatccctttc ggaacgttta agtgcgttac 2820
aggacaaaat actagaccac tatgaaaatg acagtaaaga cataaacagc caaataagtt 2880
attggcaact tatacgtttg gaaaatgcaa tactatttac agcaagggaa catggtatta 2940
ccaaactaaa ccaccaggtg gtgcctccta ttaacatttc aaaaagcaaa gcacataaag 3000
ctattgaact gcaaatggcc ttaaagggcc ttgcacaaag caagtataac aatgaggaat 3060
ggacactgca agatacatgc gaggaactat ggaatacaga accgtcgcag tgttttaaaa 3120
aaggcggtaa aaccgtgcac gtatactttg atggcaacaa ggacaactgt atgaactatg 3180
tagtatggga cagtatatat tatataactg agacagggat atgggacaaa acagcagcat 3240
gtgttagcta ttggggtgta tattatataa aagatggaga taccacatat tatgtacaat 3300
ttaaaagcga atgtgagaaa tatggaaata gtaatacgtg ggaagtacaa tatgggggca 3360
atgtaattga ttgtaatgac tctatgtgca gtaccagtga cgacacggta tccgctactc 3420
agattgttag acagctacaa cacgcctcca cgtcgacccc caaaaccgca tccgtgggca 3480
ccccaaaacc ccacatccag acgccggcta ctaagcgacc tagacagtgt ggactcacag 3540
agcagcacca cggacgtgtc aacacccacg tgcacaaccc gctcctgtgt tcaagtacaa 3600
gtaacaacaa aagaaggaaa gtgtgtagtg gtaacactac gcctataata cacttaaaag 3660
gtgacaaaaa cagtttgaaa tgtttaagat ataggctacg caaatatgca gaccattact 3720
cagaaatatc ctccacctgg cattggacag gttgtaataa aaacactggt atattaactg 3780
taacatataa tagtgaggta caaagaaata cctttttgga tgtagttact attcctaaca 3840
gtgtacaaat ctcggtggga tacatgacta tatgaatctg tatattgtat acagtatgta 3900
acattactat gctatcttta gtgtttttat tgtgcttttc tgtgtgcctt tatgtgtgct 3960
gcaatgtccc gcttgtgcag tctgtctatg tgtgtgcttt tgcttggttg ttggtgtttc 4020
tttttatagt tgttattaca tccccattaa cagcatttgc tgtatacatt tgttgctatt 4080
tactacctat gtttgtatta catatgcatg ctttacacac catacaataa ttactataat 4140
gtacagtaca gtgtaacata cctgtgatgt gcatgttgtt gtatttttgt atttttgtat 4200
ttttgtattt ttgtatttta tatgtttaat aaaccatggt atcccaccgt gcagcacgtc 4260
gcaagcgggc ctctgcaact gacttatata gaacatgtaa gcaatccggt acgtgccccc 4320
ctgatgttat taacaaagtg gaaggcacaa ccttagctga taaaatttta cagtggtcta 4380
gccttgggat atttttgggt ggccttggca ttggtaccgg cagtggttct ggaggccgta 4440
cgggctatgt acccttaggg ggcaggtcta atactgttgt ggatgttggc cccactaggc 4500
cacctgtggt tattgaacct gtagggccta ctgatccatc tattgttacg ttggtagagg 4560
attccagtgt tgttgcctct ggtgctccgg ttcccacatt taccggaacc tctgggtttg 4620
aaattacgtc ttctggtact accacaccag ctgtgttgga catcacacct accgtggact 4680
ctgtttctat ttcgtcaact agttttacaa atcctgcatt ttctgatccc tctattattg 4740
aggtgcccca aacaggggag gtatcaggta atatatttgt tggtacacca acatcgggca 4800
gccatggata tgaggaaata cctttacaaa catttgcatc ttctgggtca ggtacggaac 4860
ccattagtag tacccccctc cctactgtgc ggcgggtacg gggtccccgc ctgtatagta 4920
gggctaatca acaggtccgt gtgtccacct cacagttttt aacacatccc tcatcgttgg 4980
ttacatttga taatccagct tatgagcccc tggacaccac actatccttt gagcctacca 5040
gtaatgttcc tgattccgat tttatggata ttattcgttt gcataggcca gcattatcct 5100
ctagacgtgg cactgttaga tttagtagat tgggtcaaag ggcaaccatg tttacacgta 5160
gtggtaaaca aatagggggt agggtacatt tttaccatga tataagcccc attgctgcta 5220
cagaggaaat tgaattgcag cctttaatta gtgctacaaa tgatagtgac ctgtttgatg 5280
tatatgcaga cttcccacct cctgcgtcca ctacacctag cactatacac aaatcattta 5340
catatccaaa gtattccttg accatgcctt ctactgctgc atcctcttac agtaatgtta 5400
cagtaccatt aacatctgca tgggatgtac ctatatatac tggcccggac attatattgc 5460
catcccatac tcctatgtgg cctagtacat ctcctaccaa tgcttccacc accacctata 5520
taggtattca tggcacacaa tattatttat ggccatggta ttattatttt cctaaaaaac 5580
gtaaacgtat tccctatttt tttgcagatg gctttgtggc ggcctagtga cagtacggta 5640
tatcttccac caccttctgt ggccagagtt gtcagcactg atgattatgt gtctcgcaca 5700
agcatatttt atcatgcagg cagttcccga ttattaactg taggcaatcc atattttagg 5760
gttgtaccta atggtgcagg taataaacag gctgttccta aggtatccgc atatcagtat 5820
agggtgttta gagtagcttt acccgatcct aataaatttg gattacctga ttctactata 5880
tataatcctg aaacacaacg tttggtttgg gcatgtgtag gtatggaaat tggtcgtggg 5940
cagcctttag gtattggcct aagtggccat ccattttata ataaattgga tgatacagaa 6000
agtgctcatg cagctacagc tgttattacg caggatgtta gggataatgt gtcagttgat 6060
tataagcaaa cacagctgtg tattttaggt tgtgtacctg ctattggtga gcactgggcc 6120
aagggcacac tttgtaaacc tgcacaattg caacctggtg actgtcctcc tttggaactt 6180
aaaaacacca ttattgagga tggtgatatg gtggatacag gttatggggc aatggatttt 6240
agtacattgc aggatacaaa gtgcgaggtt ccattagaca tttgtcaatc catctgtaaa 6300
tatccagatt atttgcaaat gtctgctgat ccctatgggg attctatgtt tttttgccta 6360
cgccgtgaac aactgtttgc aagacatttt tggaataggg caggtgttat gggtgacaca 6420
gtacctacgg acctatatat taaaggcact agcgctaata tgcgtgaaac ccctggcagt 6480
tgtgtgtatt ccccttctcc cagtggctct attattactt ctgattctca attatttaat 6540
aagccatatt ggttacataa ggcccagggc cataacaatg gtatttgttg gcataatcag 6600
ttgtttgtta ctgtagtgga cactacccgc agtactaatt taacattatg tgcctctaca 6660
caaaatcctg tgccaagtac atatgaccct actaagttta agcagtatag tagacatgtg 6720
gaggaatatg atttacagtt tatttttcag ttgtgcacta ttactttaac tgcagaggtt 6780
atgtcatata tccatagtat gaatagtagt atattagaaa attggaattt tggtgtccct 6840
ccaccaccta ctacaagttt ggtggataca tatcgttttg tgcaatcagt tgctgttacc 6900
tgtcaaaagg atactacacc tccagaaaag caggatccat atgataaatt aaagttttgg 6960
actgttgacc taaaggaaaa attttcctcc gatttggatc aatatcccct tggtcgaaag 7020
tttttagttc aggctgggtt acgtcgtagg cctaccatag gacctcgtaa gcgtcctgct 7080
gcttccacgt ctactgcatc tactgcatct aggcctgcca aacgtgtacg tatacgtagt 7140
aagaaataat atgttagcac atatatgtat gtttgtatgt atggttttgt atgttgtatg 7200
tatgtatgta tttgtgtgat atattactgt attttgtttg tttgcgtgcg tgtatgtatg 7260
aatgtgcctt gtggcatgta tggtgttact gtacataatt gtggtattaa ataaagtatg 7320
ctaatagtgt tgtgtagggt tgcacccttg tgagtaacaa tactatttgt gtgtatgtgt 7380
attgctttgt accctatatt ctttcctgta tttcaagtta taaacttgca tactacacag 7440
catccatttt acttataatc ctccattttg ctgtgcaacc gatttcggtt gcctgtggct 7500
tatatgtgac cttttaaaca taatacctaa actggcacat ttacaacccc tacatagttt 7560
aacctactgg cgcgccttct tggcgtacat gtggcacacc tggtattagt cattttcctg 7620
tccaggtgta ctaaaacaat ggcttgcaca actgtatcca caccctatgt aataaaactg 7680
cttttaggca catattttag tctgttttta cctgtgctaa ttgtataatt ggcgtgtaga 7740
accactttct tatccaacaa tctgtctact tgttacataa actataaact gactcactta 7800
tacatacata gtttatgcaa ccgaaaaagg ttgggcccta taacacatac cttttctt 7858
<210> 8
<211> 7808
<212> DNA
<213> Artificial Sequence
<220>
<223> Artificial Synthesis
<400> 8
aacaattatc ttgtaaaaac tagggtgtaa ccgaaaaggg ttatgaccga aaacggtgca 60
tataaaagtg cagtggtaaa agtatagaag aacaccatgt tcgaagacaa gagggaaaga 120
ccacgaacgc tgcatgaatt atgtgaagct ttgaacgttt ctatgcacaa tatacaggta 180
gtgtgtgtgt attgtaaaaa ggaattatgt agagcagatg tatataatgt agcatttact 240
gaaattaaga ttgtatatag ggataataat ccatatgcag tatgcaaaca atgtttactg 300
ttttattcaa aaattagaga gtatagacgt tatagcaggt ctgtgtatgg tactacatta 360
gaggcaatta ctaaaaaaag cttatatgat ttatcgataa ggtgtcatag atgtcaaaga 420
ccacttgggc ctgaagaaaa gcaaaaattg gtggacgaaa aaaaaaggtt ccatgaaata 480
gcgggacgtt ggacggggca atgcgctaat tgctggcaac gtacacgaca acgtaacgaa 540
acccaagtgt aataaagcca tgcgtggtaa tgtaccacaa ttaaaagatg tagtattgca 600
tttaacacca cagactgaaa ttgacttgca atgctacgag caatttgaca gctcagagga 660
ggaggatgaa gtagataata tgcgtgacca gctaccagaa agacgggctg gacaggctac 720
gtgttacaga attgaagctc cgtgttgcag gtgttcaagt gtagtacaac tggcagtgga 780
aagcagtgga gacacccttc gcgttgtaca gcagatgtta atgggcgaac taagcctggt 840
ttgcccgtgt tgtgcgaaca actagcaacg gcgatggact gtgaaggtac agaggatgag 900
ggggcggggt gtaatgggtg gttttttgtt gaagcaatag tagaaaaaaa aacaggagat 960
aatgtttcgg atgatgagga tgaaaatgca gatgatacag gatctgattt aataaacttt 1020
atagatagtg aaactagtat ttgcagtcag gcggaacagg agacagcacg ggcgttgttt 1080
caggcccaag aattacaggc aaacaaagag gctgtgcatc agttaaaacg aaagtttcta 1140
gtcagcccgc gaagcagccc attaggagac attacaaatc aaaacaacac acacagccat 1200
agtcaggcaa acgagtcaca agttaaaagg agattactgg acagttatcc ggacagcgga 1260
tatggcaata cacaagtgga aactgtggaa gcaacgttgc aggtagatgg gcaacatggc 1320
ggttcacaga acagtgtgtg tagtagcggg gggggcagtg ttatggatgt ggaaacaaca 1380
gaaagctgtg caaatgtaga actaaacagt atatgtgaag tattaaaaag cagtaatgca 1440
aaagcaacgt taatggcaaa atttaaagag ttgtatggta ttagttataa tgagttggta 1500
cgggtgttta aaagtgataa aacatgttgt atagattggg tttgtgcatt gtttggcgtt 1560
tccccaatgg tagcagaaaa tttaaaaaca ctaattaagc cattttgcat gtactaccat 1620
atacaatgtt tatcatgtga ttggggcacc attgtattaa tgctaattag gttttcatgt 1680
gcaaaaaaca gaacaacaat tgctaagtgt ttaagtacat tagtaaatat cccacaatca 1740
caaatgttta tagaaccacc aaaattacgt agtacacctg tggcattata tttttataga 1800
acaggcatat caaacattag caatacatat ggagagacac ctgaatggat tacacgacaa 1860
acgcaactac aacatagttt tgaggatagt acctttgaat tatcacaaat ggtgcaatgg 1920
gcatttgacc atgaagtatt agatgatagt gaaatagcat ttcattatgc acaattagca 1980
gatatagata gtaatgctgc agcgttttta aagagtaatt gccaagcaaa atatgtaaaa 2040
gattgtggga ccatggcacg gcattacaaa cgagcacaaa gaaaatcatt atctatgtca 2100
gcctggataa ggtatagatg tgatagagca aaggatggag gcaactggag agaaattgct 2160
aaatttttaa gatatcaagg tgtaaacttt atgtccttta ttcaaatgtt taaacagttt 2220
ttaaaaggaa caccaaaaca caattgcata gtcatatatg gcccaccaaa cacaggcaag 2280
tcattatttg caatgagcct aatgaagttt atgcaagggt ccattatttc atatgtaaac 2340
tctggtagtc atttttggtt acagccacta gaggatgcta aaatagcatt gttagatgat 2400
gctacgtatg ggtgttggac atatattgat cagtatttaa gaaacttttt agatggtaat 2460
ccatgtagta tagatagaaa acataggagt ttaatacaat tagtatgtcc accattacta 2520
ataacgtcaa acataaatcc acaagaggat gcaaacctaa tgtatttaca tacaagggta 2580
acagtattaa agtttttaaa tacatttcca tttgataaca atgggaatgc tgtgtataca 2640
ttgaatgatg aaaattggaa aaattttttt tccaccacat ggtccagatt agatttggag 2700
gaggaagagg acaaagaaaa tggagaccct atgccaccgt ttaaatgtgt gccaggagaa 2760
aatactagac tgttatgaac tggacagtga taaattagta gatcaaatta actattggac 2820
attgttacga tatgaagctg ctatgtttta tgcagcacgg gaaagaaact tacgaacaat 2880
caatcaccag gtagtaccag caacaacagt atcaaaacaa aaggcctgtc aagcaattga 2940
aatgcacatg gccttacaat cgcttaacaa atcagactat aacatggaac catggacaat 3000
gcgggagaca tgttatgaac tatggtgtgt ggctcccaag caatgtttca aaaagggggg 3060
cataactgta acagttatat ttgatggaaa taaggacaat gcaatggact atacaagctg 3120
gaaatttata tatatatatg ataatgataa gtgggtaaag acaaatggaa atgtggacta 3180
tacgggtata tattacactg taaattcaaa aaaagaatat tatgtacagt ttaaagatga 3240
agccaaaata tatggggcac aacagtggga ggtctatatg tatggtactg taataacatg 3300
tcctgaatat gtatctagta cctgcagcga cgcgttatcc actactacaa ctgttgaaca 3360
actatcaaac accccaacga ccaatcccct taccacctgc gtgggcgcca aagaagccca 3420
gacacaacag cgaaaacgac agcgacttac tgagcccgac tcctccacaa tctccccact 3480
gtccgtggac aatacaaaca accaaataca ctgtggaagt ggaagcacta acactggagg 3540
gcaccaaagt gcaactcaga ctgcgtttat agtgcattta aaaggtgata caaattgttt 3600
aaaatgtttt agatacagat ttacaaaaca caaagggtta tataaaaacg tatcctcaac 3660
ctggcattgg accagtaata ctaaaacagg cattgttacc attgtgtttg acagtgcaca 3720
tcaacgggaa acatttataa aaaccattaa agtaccccca agtgtaacac tgtcattggg 3780
aattatgaca ctgtaactag tgtaatatat gtattgtaca tatatactgt cacaagccaa 3840
tatgtgctgc taattgtata gacatattgt aaccattgca gtgtttatta ttttgctatt 3900
tgtgctttgc ttgtgtgtgt gtcttgtgtt gtgttgtttg ttgccgctac tgctgtccca 3960
atacgtgttt gcagctgcct tattattaat tttatgtttt tggtttgttg ttgcaacatc 4020
ccaattaact acattttttg tatatttgat ttttttttac ttaccttgtt tacttttaca 4080
tctatataca tttttacttt tgcaataaac ttgttatatt tttgtgatta aatatggtgg 4140
ctacacgtgc acggcgtcgg aagcgagcat ctgtaacaca attatattct acatgcaaag 4200
ctgctggtac atgtcctcct gatgttgtga ataaggttga aggtactaca ttggccgata 4260
aaatattaca gtggagtggg ttgggtatat ttttgggtgg cctaggtatt ggtactgggt 4320
ctggatctgg ggggcgtact ggatatatcc ctttaggtgg tgggggtcgc ccaggcgtgg 4380
tggatattgc tcctgcaagg ccacctatta taattgacct atggcaccat actgaacctt 4440
ctatagtaaa tttggttgag gactctagta ttattcagtc tgggtctcct atacctacct 4500
ttactggtac cgatggcttt gaaattactt catcttccac aacaacccct gctgtgttgg 4560
acatcacccc atctgctggt actgtacatg tttctagtac taacattgaa aatcctttat 4620
atattgaacc tccatccatt gaggctccac aatctggaga agtgtcagat atatatttac 4680
tagtacacta ctctggtact catgggtatg aagaaatacc tatggaagtg tttgcatcca 4740
atgtcagtac tggtactgaa cctattagca gcacacctac tccaggggtt agtcgcatag 4800
ctgctccccg cttgtatagt aagtcctaca cacaggttaa agttacaaat cctgatttta 4860
ttagtaagcc atccacattt gttacattta ataatcctgc ttttgagcct attgacacat 4920
ccataacttt tgaggaacct gatgctgttg cacctgatcc tgattttctg gatattatta 4980
cactgcaccg ccctgccctt acatctcgta gaggcacagt acgctttagt aggttaggtc 5040
aaaaggccac catgcgcact cgtagtggca aacaaattgg tgctcgtgta cattattatc 5100
atgatattag tagaattgca ccagctgatg aacttgaaat gcagccttta ctttcacctt 5160
ctaataatta tagttatgac atttatgctg atttagatga agctgaaaca ggttttatac 5220
agcccacaca caccacacct atgtcacact cctctttgtc taggcagttg ccctccttat 5280
cttcatctat gtcttcatct tatgcaaatg ttactattcc attttcaact acatattctg 5340
ttcctattca tacagggcct gatgtggtat tgcccacatc tcctacagta tggccttatg 5400
ttccccacac ttccattgac accaagcatt ctattgttat actaggtggg gattactatt 5460
tgtggcccta tacacattta ctacgcaaac gccgtaaacg tataccctat ttttttacag 5520
atggcattgt ggcgcactaa tgacagcaag gtgtatttgc cacctgcacc tgtgtctcga 5580
attgtgaata cagaagaata tatcacacgc accggcatat attactatgc aggcagttcc 5640
agactaataa cattaggaca tccctatttt ccaataccta aaacctcaac gcgtgctgct 5700
attcctaaag tatctgcatt tcaatacagg gtatttaggg tacagttacc agatcctaac 5760
aagtttggac tcccggatcc aaatttatat aatccagaca cagataggtt ggtgtggggt 5820
tgtgtgggcg ttgaggtggg cagaggacag ccccttggtg ttggccttag tggtcatccc 5880
ttatttaata aatatgatga cacagaaaat tcacgcatag caaatggcaa tgcacaacaa 5940
gatgttagag ataacacatc tgttgacaac aaacagactc agttatgtat aataggctgt 6000
gctccaccta ttggggaaca ctggggtatt ggcactacat gcaaaaacac acctgtacct 6060
ccaggagact gcccccccct ggaacttgta tcctctgtca ttcaggatgg cgatatgatt 6120
gatacagggt ttggagctat ggatttcgct gccctacagg ccaccaaatc agacgtccct 6180
ttggatattt cacagtctgt ttgtaaatat cctgattatt taaaaatgtc tgcagacaca 6240
tatggtaatt ccatgttttt tcatttacgc agggagcaaa tctttgctag gcactattat 6300
aataaacttg taggtgttgg ggaagacatt cctaacgatt attatattaa gggtagtggt 6360
aatggccgtg accctataga aagttatata tactctgcta ctcccagtgg gtctatgata 6420
acatctgatt ctcaaatttt taataagcct tattggctcc accgtgcgca gggtcacaat 6480
aatggcattt gctggaacaa tcagcttttt attacctgtg ttgatactac cagaagtaca 6540
aatttaacta ttagcactgc cactgctgcg gtttccccaa catttactcc aagtaacttt 6600
aagcaatata ttaggcatgg ggaagagtat gaattgcaat ttatttttca attatgtaaa 6660
attactttaa ctacagaggt aatggcttat ttacacacaa tggatcctac cattcttgaa 6720
cagtggaatt ttggattaac attacctccg tctgctagtt tggaggatgc atataggttt 6780
gttagaaatg cagctactag ctgtcaaaag gacacccctc cacaggctaa gccagatcct 6840
ttggccaaat ataaattttg ggatgttgat ttaaaggaac gattttcttt agatttagac 6900
caatttgcat tgggtcgcaa gtttttgttg caggttggcg tacaacgcaa gcccagacca 6960
ggccttaaac gcccggcctc atcggcatcc tcttcctctt cctcttcagc caaacgtaaa 7020
cgtgttaaaa agtaatgtat gttagttttt gtatgcttgt gcacactgtt gtatgcctgt 7080
atgtatatgt ttgtgtatgt actgtatgtg tttttgtgtg tgtgtgtgtt gttgttcctg 7140
tatgtatgag ttatgtatgt ttattattaa taaactatgt ggtgtgtgtg tgtgtgtttt 7200
tgcatgactg catttgtatg acatgtacgg gtgtatgtgg gtattacatt atccccgtag 7260
gtcaagggtg gtgtttcggt ggcgtcccta ttgccctacc cattttttgc agcacaacag 7320
tttatatttg tgctatttag ttatactttg tagcttccat tttgttacag ctgcagccat 7380
tttgagtgca accgatttcg gttcgtgtac ttttagtata tttgccaagt tttaaaccac 7440
aactgccagt tgtttttggc ataaaccatc atttttttat gacatagtgc atacatccgc 7500
ccgcccacgc cttgtacttg gcgcgcctta ccggcgctag tcatacaacc tattagtcat 7560
ttgtacttta acaattgttg gcacactgtt ttccgcccta taataattta actgcttata 7620
ggcatgtatt ttttggcata ttttatctta ctaattgcat agttggcagg tcaaatacta 7680
tgtttttagt gccaagtttc tatcctactt ataaaccatc ttactcatat gcaggtgtgc 7740
tacacaaatg tgttacctaa ccgatttgtg ttctgcctat gcttgcaaca ttttttctta 7800
taacattt 7808
<210> 9
<211> 7942
<212> DNA
<213> Artificial Sequence
<220>
<223> Artificial Synthesis
<400> 9
taaattataa tcttatacta gtaaaaaata gggtgtaacc gaaaacggtc agaccgaaac 60
cggtgtatat atatagaaca cagtgtagct aacgcacggc catgtttgag gatccagcaa 120
cacgaccccg gaccctgcac gaattgtgtg aggtgctgga agaatcggtg catgaaataa 180
ggctgcagtg tgtgcagtgc aaaaaagagc tacaacgaag agaggtatac aagtttctat 240
ttacagattt acgaatagta tatagagaca ataatccata tggcgtgtgt attatgtgcc 300
tacgcttttt atctaagata agtgaatata ggcattatca atattcactg tatgggaaaa 360
cattagaaga gagggtaaaa aaaccattaa gtgaaataac tattagatgt ataatttgtc 420
aaacgccatt atgtcctgaa gaaaaagaaa gacatgttaa tgcaaacaag cgatttcata 480
atattatggg tcgttggaca gggcgctgtt cagagtgttg gagaccccga cctgtgaccc 540
aagtgtaacg tcatgcgtgg agacaaagca actataaaag attatatatt agatctgcaa 600
cctgaaacaa ctgacctaca ctgctatgag caattaggtg acagctcaga tgaggaggat 660
acagatggtg tggaccggcc agatggacaa gcagaacaag ccacaagcaa ttactacatt 720
gtgacatatt gtcacagttg tgatagcaca ctacggctat gcattcatag cactgcgacg 780
gaccttcgta ctctacagca aatgctgttg ggcacattac aagttgtgtg ccccggctgt 840
gcacggctat aaacaaccct gcaatggagg accctgaagg tacagagggc gaaagggagg 900
gatgtacagg ctggtttgaa gtagaggcaa taatagaaaa acaaacagga gataacattt 960
cagaggacga ggatgaaaat gcatatgata gtggaacaga tctaatagat tttatagatg 1020
attcaaatat aaataatgaa caggcagaac atgaggcagc ccgggcattg tttaatgcac 1080
aggaagggga ggatgattta catgctgtgt ctgcagtaaa acgaaagttt acaagcagtc 1140
cggaaagtgc tgggcaagat ggtgtagaaa aacatggtag tccgcgtgca aaacacattt 1200
gtgtaaatac agagtgtgtt ttaccaaaac gcaaaccatg tcacgtagaa gacagcggct 1260
atggcaatag tgaagtggaa gcgcagcaga tggcagacca ggtagacggg caaaatggcg 1320
actggcaaag taacagtagt caatcaagtg gggtgggggc tagtaattca gatgtaagtt 1380
gtactagtat agaggacaat gaggaaaata gtaatagaac gctaaaaagc atacaaaata 1440
ttatgtgcga aaatagcata aaaacaactg tattatttaa atttaaagaa acatatggtg 1500
ttagctttat ggaattagta agaccattta aaagtaatag aagtagttgt acagattggt 1560
gtattatagg aatgggagta acaccatcag ttgcagaagg attaaaagta ttaatacagc 1620
cctatagcat atatgcccat ttgcaatgtt taacatgtga cagaggcgtg cttatactgc 1680
tgctaattag gtttaaatgt ggaaaaaaca gattaacagt gtccaaacta atgtcacagc 1740
tgttaaatat accagaaaca catatggtaa tagaaccacc aaaattacga agtgctacct 1800
gtgcattata ttggtataga acaggtttgt ctaatattag tgaggtatat ggtaccaccc 1860
cagaatggat agaacaacaa acagtattac agcatagctt tgacaatagc atattcgatt 1920
ttggagaaat ggtgcaatgg gcatatgatc atgatataac agatgatagt gacatagcat 1980
ataaatatgc acagttagca gatgtaaata gcaatgctgc agcattccta aaaagcaatt 2040
cgcaagcaaa aatagtaaag gactgtgcaa ccatgtgtag acattataaa cgggcagaaa 2100
gaaaacatat gaatattgga caatggatac agtatagatg tgatagaata gatgatggtg 2160
gagattggag gcctatagta agatttttaa gatatcaaga catagaattt acagcctttt 2220
tagacgcatt taaaaaattt ttaaaaggta tacctaaaaa aaattgttta gtattatatg 2280
gacctgcaaa cacaggaaaa tcatattttg gaatgagttt aattaggttc ttaagtggat 2340
gtgtaatatc ctatgtaaac tcaaaaagcc atttttggct acaaccatta acagatgcaa 2400
aagtgggtat gatagatgat gtaacaccta tatgttggac atatatagat gattatatga 2460
gaaatgcact ggatggaaat gatatatcag tagatgtaaa gcatagagcc ttagtacaaa 2520
taaaatgccc accattaatt ttaacaacaa atacaaatgc aggaacagat cctaggtggc 2580
catatttaca tagtagattg gttgtgtttc atttcaaaaa cccatttcca tttgatgaaa 2640
atggcaatcc tatatatgaa attaacaacg aaaattggaa atcctttttc tcaaggacgt 2700
ggtgcaaatt agatttaata caggaagagg acaaggaaaa cgatggagtc gataccggca 2760
cgtttaaatg cagtgcagga aaaaatacta gatctatacg aagctgatag taatgaccta 2820
aacgcacaaa ttgaacattg gaaattgact cgaatggaat gtgttttgtt ttacaaagca 2880
aaggaactgg gaataactca tataggccac caggtggtgc caccaatggc agtgtctaag 2940
gcaaaggcct gccaagctat tgaactacaa ttggcattgg aggcattaaa caaaacacaa 3000
tatagcacag atggatggac attacaacaa acaagtctag aaatgtggcg tgcagaacca 3060
caaaaatact ttaaaaaaca tgggtataca ataacagtgc aatacgataa tgataaaaac 3120
aatactatgg attatacaaa ctggaaggaa atttatttac ttggtgagtg tgaatgtaca 3180
attgtagaag gacaagtaga ttactatggg ttatattatt ggtgtgatgg agaaaaaata 3240
tattttgtaa aatttagtaa cgatgcaaag caatattgtg taacaggagt atgggaagta 3300
catgtgggtg gtcaggtaat tgtttgtcct gcatctgtat ctagtaacga agtatccact 3360
actgaaactg ctgtccacct atgcaccgaa acctccaaga cctccgcagt gtccgtgggt 3420
gccaaagaca cacacctaca accaccacag aaacgacgac gaccagacgt cacagactcc 3480
agaaacacca agtaccccaa caaccttttg cggggacaac aatccgtgga cagtactaca 3540
cggggactcg tcactgcaac tgagtgcaca aacaaaggac gggttgcaca tacaacttgt 3600
actgcaccta taatacacct aaaaggtgat cctaatagtt taaaatgttt aagatatagg 3660
gtaaaaacac ataaaagttt gtatgttcaa atttcatcta cctggcattg gaccagtaat 3720
gaatgtacaa ataataaact aggtattgta acaataacgt acagtgatga aacacaacgt 3780
caacaatttt taaaaactgt taaaatacca aatactgtgc aagttataca aggtgtcatg 3840
tcattgtgat atttgtacat atgtatatat gtatatgtgt atggtaaaca cccaacacaa 3900
gccaatattg ctgctattgt gtatatataa caatgttagg attatttgta ttttgtttta 3960
ttttgcttat ggtgttttgt gcagtgctta ggccgctctt gctatctata tcggtgtatg 4020
cgcaggtgtt ggtgctggtg cttttgctat gggtatctat tgggtcacca tttaaagtgt 4080
tttttttgta cctactgttt ttatattttc caatgttttg tattcactgt catgcacagt 4140
atttggcaca actgcaataa ctgtacatgt agattggcta catgcatata tgcaaaatat 4200
actttttcac ttttgtagtt tgtctaataa atacttttat attttttaat agcttgtcgc 4260
aatgagatac agacggtcta cacggcacaa acgtgcttct gcaacacagc tatatcaaac 4320
atgcaaagcc tctggcacct gcccccccga tgttattcct aaagtggaag gcacaactat 4380
tgcagatcaa cttttaaaat atggcagcct aggggtgttt tttggaggtt tgggtatagg 4440
tacaggtgca ggctctggtg gtagggcagg ctatgtgcca ttgtccactc gtcctcccac 4500
tagtagtatt accacgtcca ccattcgtcc ccctgtaact gtagaaccca ttggtccctt 4560
agaaccatct atagtttcta tgatagaaga aacaacattt attgagtctg gcgcacctgc 4620
tccatctatt ccatcagcaa cagggtttga tgttacaaca tctgcaaata atactcctgc 4680
aataattaat gtaacatcta taggtgaatc atctgtacaa tcagtttcta cacatttaaa 4740
tcctacattc actgaaccat ctataataca gcccccggca cctgcagaag catctggtca 4800
tgtattgttt tctagtccaa ctattagtac acacacctat gaagaaatcc ctatggatac 4860
atttgttacc tctactgaca gcagcagtgt aacaagtagt acacctattc cagggtctcg 4920
ccctacgaca cgccttggtt tatatagccg tgccacacaa caggttaagg tagtcgaccc 4980
tgcttttatg tcatcaccac agaaattagt aacatataac aatcctgttt ttgagggcgt 5040
tgatacagat gaaactataa tttttgatcg ttcacaactt ttacctgcac cggatcctga 5100
ttttttagac attatagctt tgcataggcc tgcattaacc tctcgaagag gtactgttag 5160
gtttagcagg cttggtaata aggccaccct acgtacacgt agtggaaaac aaattggggc 5220
acgggtacat tattatcatg atattagtcc tatccagcct gctgaagttc aggaagacat 5280
agaattgcaa cctttattac cacagtctgt gtccccttac actattaatg atggtttgta 5340
tgatgtgtat gcagattctt tgcagcaacc cacgtttcac ttaccttcca cactttctac 5400
ccataataat actttcactg tacctattaa tagtggtatt gactttgtat atcaacccac 5460
tatgtccatt gagtcaggtc ctgacattcc attaccttcg ttacccacac atactccttt 5520
tgttcctata gcccctacag ctccatctac atctattatt gttgatggta cagattttat 5580
tttacatcct agttattttt tactacgtcg caggcgtaaa cgttttccat atttttttac 5640
agatgtccgt gtggcggcct agtgaggcca ctgtgtacct gcctcctgta cctgtctcta 5700
aggttgtaag cactgatgag tatgtgtctc gcacaagcat ctattattat gcaggcagtt 5760
ctcgattact aacagtagga catccctatt tttctattaa aaacaccagt agtggtaatg 5820
gtaaaaaagt tttagttccc aaggtgtctg gcctgcaata cagggtattt agaattaaat 5880
tgccggaccc taataaattt ggttttccag atacatcttt ttataaccca gaaacccaaa 5940
ggttggtgtg ggcctgtaca ggcttggaaa ttggtagggg acagccttta ggtgtgggta 6000
ttagtgggca tcctttatta aacaagtttg atgatactga aaccagtaac aaatatgctg 6060
gtaaacctgg tatagataat agggaatgtt tatctatgga ttataagcag actcagttat 6120
gcattttagg atgcaaacct cctataggtg aacattgggg taagggaacc ccttgtaata 6180
ataattcagg aaatcctggg gattgtcctc ccctacagct cattaacagt gtaatacagg 6240
atggggacat ggtagataca ggatttggtt gcatggattt taataccttg caagctagta 6300
aaagtgatgt gcccattgat atatgtagca gtgtatgtaa gtatccagat tatttgcaaa 6360
tggctagcga gccatatggt gacagtttgt tcttttttct tagacgtgag caaatgtttg 6420
ttagacactt ttttaatagg gccggtacct taggtgaccc tgtgccaggt gatttatata 6480
tacaagggtc taactctggc aatactgcca ctgtacaaag cagtgctttt tttcctactc 6540
ctagtggttc tatggtaacc tcagaatccc aattatttaa taaaccgtac tggttacaac 6600
gtgcgcaggg ccacaataat ggcatatgtt ggggcaatca gttgtttgtc acagttgtgg 6660
ataccactcg tagcactaac atgactttat gtgctgaggt taaaaaggaa agcacatata 6720
aaaatgaaaa ttttaaggaa taccttcgtc atggcgagga atttgattta caatttattt 6780
ttcaattgtg caaaattaca ttaacagctg atgttatgac atacattcat aagatggatg 6840
ccactatttt agaggactgg caatttggcc ttaccccacc accgtctgca tctttggagg 6900
acacatacag atttgtcact tctactgcta taacttgtca aaaaaacaca ccacctaaag 6960
gaaaggaaga tcctttaaag gactatatgt tttgggaggt ggatttaaaa gaaaagtttt 7020
ctgcagattt agatcagttt cctttaggta ggaagttttt gttacaggca gggctacagg 7080
ctaggcccaa actaaaacgc cctgcatcat cggccccacg tacctccaca aagaagaaaa 7140
aggttaaaag gtaaccattg tctgttgggt aattgtctgt gtcatgtatg tgttgtgtat 7200
gtcaaacaca ggttaaaagg taaccattgt ttgttatgta attgttttgt gtgtgtactg 7260
tgttgtttgc atgttatgta tgtgtgtgca tgtttgttgt atttgtcagt tcctgtatgt 7320
atgttttgtg tatgtattaa taaagtactg tatttactaa actatttata gtagtcttat 7380
gttatgttat ggttgcaccc acatgagtaa caatacagtt gctcctaatc tattgcatct 7440
cctgccctac cctgtgtccc ctgccctacc ctgtgtccta ctttgttaca ctactaatta 7500
gccttatact ctccattttg taccattttg tactatccac cattttaaat cctaaccgaa 7560
ttcggttggt cttggcacaa ctttggttgt ccttggcaca gtaacaacta tttttatata 7620
agtttcagca aactgcttaa tcctttggtt tcctgcagtc cactggtcta cacttgttgt 7680
cccgcctaaa ctgacttctt gctgactcac aggtcctgca gtgcagctaa acaatacatt 7740
gcctaacatt gcatgtttta aactgctttt aggcacatat tttatttaaa ctttcaatgc 7800
actaattaca gtgttggctt acacaagtac atcctacgcc aaatatgtct tgtaaaacat 7860
gattaaatac tgttactcac caggtgtgca ctacacgacc ggttacggtt accgtaccca 7920
caaccacttt tttttataat ta 7942
<210> 10
<211> 7859
<212> DNA
<213> Artificial Sequence
<220>
<223> Artificial Synthesis
<400> 10
gaaagtaaca atcctacttt tatagacagg gagtaaccga aataggttta ggaccgaaaa 60
cggtacatat aaaagcactg tgtacaacac ccaggacatc catggatcgt cagttatttg 120
aaaatacaga agagcgacca cgtacattgc accagctatg tgaagttgtg aataaaccat 180
tgctggagct gcaacttggc tgtgtgttct gcaagaaggc attgacagcg tcagaggtat 240
ataattttgc atatacagat ctaagagtag tgtatagaga cgggtatccg tatggagtgt 300
gcaaattctg tttgctattt tatagtaagg tccgaaaatt aagatattac aattgttcag 360
tgtacggggc tagcctggaa gcactaacta aaaaaaagtt atctgattta tcaataaggt 420
gctacagatg tcaacatccg ttgacaccag aggaaaaaca gttacactgt gactataaga 480
aacggtttca caaaatttca catatgtgga ccgggtcgtg cctgacatgc tggagacaca 540
caacagcaac tgaatcagca gtataatcat gcacggtaac gtaccaacac ttccacaata 600
tattatagaa cttataccac aaactgagat tgacctgcaa tgccatgagc aattgaacag 660
ctcagaggat gaggatgagg atgaagtaga ccatctgcag gagcagccac agcaagctag 720
acgggacgaa caacatcctt gttacctaat tgaaacacag tgttgtaggt gtgagtcgtt 780
ggtgcagttg gctgttcaga gttcaacaaa agagctgcgt attttacaac aaatgcttat 840
gggcacagtg gagcttgtgt gccccctctg cgcaacaagg cgataactgc aatggcgtca 900
cctgaaggta cagatgatga ggggggatgc cggggatggt ttcacgtgga ggcaatagta 960
aaaaaacgta caggggatgt aatatctgaa gatgaaacag acgaggaaag caccgaatct 1020
gatttggatg ggtttataga caatagtaat ataatatcta cacaggcaga aagggagaca 1080
gctcagcagt tgttacatgc ccaaaacaca catgcagata cacagacgct gcagaaatta 1140
aaacgaaagt atttaggcag tccattaggt gatattagta atcagcaaac agtgtgccgg 1200
gaagctgtaa aacgaaggct aattgataca gaagtgccgg acagcgggta tggcaatact 1260
ttggaaacag tggaagcaac gcaacaggta caggaacagt atgtaaggga ggcaagtggc 1320
gggaaagagc aaaatggagg ctcacaacat agtgtgtgta gcagagatgg gagcataggg 1380
tcaggaagtg atatggatgt ggatagacag gatataatgc cactgcaaca aatacaagat 1440
atattaaaat gtagtaatgt gcaggccaag ctatattgta aatttaaaga tatatttggc 1500
ataccatttt cagagttggt acgaacattt aaaagtgata gtacatgttg tcatgattgg 1560
atatgtgcta tatttggagt aaatgaaaca ttagcagagg cattaaaaac aattattaaa 1620
acacagtgca tatattacca tatgcaatgt ttaacatgta catggggcgt ggttatatta 1680
ttgttaatta gatatacgtg tggcaaaaac agaaaaacaa ttgtaaagtc tttagcatcc 1740
atattaaatg taccaactga acaaatgctt gtgcagcctc caaaaatacg tagccctgcg 1800
gttgcattat acttttataa aacatctata tccaacatta gtgacgtgta tgggagtaca 1860
ccagaatgga tagaaagaca aacacagtta caacatagct ttgaggactg tcaatttgaa 1920
ctatctaaaa tggtgcagtg ggcatttgac aatgaagtaa ccgatgatag tcaaatagca 1980
tttcattatg cacagttagc agatgtagac agtaatgcac aagcattttt aaaaagtaat 2040
atgcaagcaa aatatgtaaa agactgtgga ataatgtgta gacactataa acgggcacaa 2100
caacaacaga tgaatatgaa acaatggata aagcatgtat gtagcaaggt ggatgatggt 2160
ggggactgga agccaatagt acagttccta aggtatcaag gggtcgagtt tatttcattt 2220
ttaagttatt ttaaattatt tttacaaggt acaccaaaac ataattgttt agttatttat 2280
ggtcctccaa acacgggtaa atcatgtttt gctatgagtc ttataaactt ttttcatggg 2340
tcagtcattt catatgtaaa ttcacacagt cacttttggt tacaaccgtt agacaatact 2400
aaattaggta tgctagatga tgccacagag gcatgttgga aatatattga tgaataccta 2460
agaaatttat tagatggcaa tcctgtaagc ttagatagaa aacataagca actagtacaa 2520
ataaagtgtc cacctgtact aataacaact aatataaatc caatgcaaga tgcaaagcta 2580
cggtatttgc acagtagaat acatgtgtta cagtttttaa atccatttcc aattgatgtg 2640
aatggaaatc ctgtatacca attaaataat gcaaactgga aatgtttttt tgaaaggaca 2700
tggtccagat tagatttgga taacgacgaa gacaaggaaa acgatggaga cgctatgcca 2760
acgtttagat gcgtgccagg agaaaattct agactgtttt gaaagagata gcaaaaatat 2820
tacagaccat atagactact ggaaagctgt gcgacaagaa aatgtaatat attataaggc 2880
aagagaaaat aatatgacta aactaggcca ccaggtggtg ccctgtttac aagtgtgtaa 2940
agcaaaggca tgcgttgcta tagaactaca aatagcattg gaatcacttt gtaaaacaga 3000
atataatatg gaagagtgga cattaaggga tgtatgtgaa agtatgtggt atacagaacc 3060
caaacagtgt tttaaaaaac aaggacaaca tatagaagtg tggtttgatg gcagcaagga 3120
caatcgggct gaatatgttg tgtggaaatg ggtatattat tgtggggagg atgggtggtg 3180
taaagtgtct tctgcagtaa gctatgaggg catatattat atacatgacg gccataaaac 3240
gtattataca aactttaaag acgaggccac caaatatggg tgtaaaggca catgggaagt 3300
gcatatggga aaacaaagta tttattgtcc cgactctgtg tccagtacct ttagatccaa 3360
cgtatcctct gttgaaactg ttaacgaata ctactcccat aagaccccca ccacctccac 3420
gcccgtgggc acctacgaag cctcatcatc cctgcggccg ggaaaacgtc ccagaaccac 3480
agagcccgac agtactgact ccaccacaca gtccactacc acagccagag agtcctacgc 3540
agagtgtgtc gcaaggaaca cagacaacac aaacaacaac accagaaaac acctccctgg 3600
tggagcttcg tgtaacaaca ccgaaatcga cagtggttat aagactgcac ctgtagtaca 3660
tataaaaggt gaagcaaaca gactaaagtg tttaaggtat cggtttcaaa aacataaaca 3720
actgtttgtt actgtatcat ccacctatca ttggacaaat gtaaactgtg ctgtaaataa 3780
tagttatatt actgttgtat ataaagatga aacccaacgc caaaaatttt tggatattgt 3840
aaaaataccc cctagtgtat cactggtact gggacatatg acatgtgttg atatgtaatg 3900
ttaatgtaat tgttacatta tgtaatattg tattacaata atacctggta tatacaatac 3960
tacacacgcc atatatgctg ctaggcctgt aaacttgcaa ccattgtgtt gtgtgttagt 4020
tttgtgtttt tgctttgctg ctttgtgtgc ctgtttgtct gctttaatgt gccgctattg 4080
cagtctgcct ctgtgtttgc ctcgtgtata ttacttatac ttttattttg gtttgttgtg 4140
gcatcatcct acataaccac atttacacta tatatacttt ttttttactt ccctttatta 4200
tgtttctata gccatgctgt gtggttaatt aacacacaat aaatcacagt ttttttgtat 4260
tgtatgtatt tgtgtgttat atataatggt tgcccaccgg gcacgcagac gtaagcgggc 4320
atcagcaacg cagctatatc aaacatgtaa acaatctggc acatgtcctg aggatgttat 4380
taacaaaatt gaacacaaaa cctgggctga taaaatattg caatggggta gtttatttac 4440
attttttgga ggccttggta ttggcactgg ctctggcact ggggggcgta caggatatat 4500
tcctttgggt actaggcctt ccactgttgt tgatgtaacc cctgctcggc cccctattgt 4560
tgtagaatcg gtaggcccca ctgacccttc aattgttaca ttggttgaag aatccagtgt 4620
tattgagtct ggagcttcgt tccctaattt tacaggtact gcaggatttg aagtaacatc 4680
ctcctcaact accacacctg cagtattaga tataacaccc acctctacct ctgtacatgt 4740
tagttctact acatatacta atcccacatt tgtagaccct cctgttattg aggtccccca 4800
aactggtgag gtatctggga atatattgat aagtacccca acatctgggg ttcatagtta 4860
tgaggaaata cctatgcaaa catttgctgt tcagggtaca ggcaatgaac ctatcagtag 4920
tactcctatt cctggtctta ggcgtatagc tgccccacgt ttatataaaa aagcctttca 4980
acaggttaag gtcacagatc ctgcatttct tcacaaacct gaaactttaa ttaatgttga 5040
taatcccata tttcaaaatg ctgacacaac gttaaccttt tcaccatcag gtgtggcacc 5100
tgatcctgat tttttagaca ttgtggcttt acacagacct gcctttacaa cacgtagagg 5160
tggtgtgcgt tttagtaggc ttggcacaaa ggccactatg cgcaccagaa gtggcaaaca 5220
aataggtgct cgtgtacatt attattatga tgtgagtcct attacacaga ctgaagaaat 5280
tgaaatgcag ccattattgt ctacagataa tacatttgat ggcctatatg atatttatgc 5340
aaacatagat gatgaggcac ctgtgtcatc tcgtttttct attgctacac cttctaggtt 5400
gcctaccaac actgttcctt tgtcttttag tggtagtacc tctaatgtta caataccctt 5460
tggtacatct tgggatgttc ccatctattc aggtcccgat gtagtgttgc ccacaggacc 5520
ccctacatgg ccttatgcac ctcaatctcc ttttgatacc acccatgatg tagttataca 5580
gggctccaca tttgcgttat ggcctgttta ttttttgaaa cgtaggcgtc gtaaacgtat 5640
tccctatttt cttgcagatg gcggtgtggc ggcctagtga cagcaaggtt tatctgcctc 5700
ctacccctgt atcaaaggtc attacaacgg atgcctatgt aaaacgcacc actatatttt 5760
atcatgctgg aagctctcgc ttgcttaccg tgggacatcc ttattacccc atttctaaat 5820
ctggtaaagc agacatccct aaggtgtctg catttcagta tagggtgttt agagtacgcc 5880
tcccagatcc taataagttt ggccttcctg atacaaatat atttaatcct gaccaggagc 5940
ggcttgtatg ggcctgtgta gggctagaga ttggccgtgg acagccttta ggtgttggtg 6000
taagtggcca tccactgttc aataggttgg atgacactga aagctctagt atagctattc 6060
aggatactgc cccggacagt agggataatg tttcagtgga tcctaaacaa acacagttat 6120
gcattattgg ctgtgcaccc gctataggtg aacactggac aaagggtacg gcctgtcgtt 6180
ccacacctac tacagcgggc gactgccccc cattggaact tatcaattca cctattgagg 6240
atggagacat ggtggacaca ggttttggtg cattaaactt taaagctttg caggaatcta 6300
aatctgatgt gccattggat attgtacaat ccacatgtaa atatcccgat tatttgaaaa 6360
tgagtgcaga tgcctatggg gattctatgt ggttttattt acgtagggaa cagttgttta 6420
ccagacattt ttttaatagg gcaggcgtta ttggtgagga aatacctaat gacttatata 6480
ttaagggtag taatggcagg gacccgcccc ctagctctgt atatgttgct acacctagtg 6540
ggtctatgat aacttcagag gctcaattgt ttaataagcc atattggctg caacgtgccc 6600
agggacataa taatggcatc tgttggaaca atcagttatt tgtaactgtt gtggatacca 6660
ccaggaatac aaacatgact ctttccgcaa ccacacagtc tatgtctaca tataattcaa 6720
agcaaattaa acagtatgtt agacatgcag aggaatatga attacaattt gtgtttcaac 6780
tatgtaaaat atccctgtct gctgaggtta tggcctattt acatactatg aattctacct 6840
tactggaaga ctggaatata ggtttgtcgc ctcctgttgc cactagctta gaggacaaat 6900
acagatatgt gaaaagtgca gctataacct gtcaaaagga tcagccccct cctgaaaagc 6960
aggacccact atctaaatat aaattttggg aggtcaattt gcaaaacagt ttttctgctg 7020
atttggatca gtttcctctt ggcaggaagt ttttaatgca ggttggggtc cgtactaaac 7080
cgcctgtatc ctctaaaaaa cgctctgctt ctactacatc tacctcagcc ccctcctcca 7140
agcgcaaacg caaatagtat gttgtgtgtc tgtattgtgt atgtatggtt gtattatgta 7200
ctatttattt attgtgtata ctgtatgtgt gtatttgttg tctgtgttgt atgactgtat 7260
gtatgtgtaa tgtttgcatg ttgttaataa atatgaatga gtgttacttt tacgcgtggt 7320
tgcataaact aaggcgcggt ggtgtcccta ggcagttggg tggcatgtta ggtggcgtcc 7380
ctgtttatat accaatctcc accctgtatt attaatatat gtactgttat catgcagtta 7440
cctataccac atatagtata gcttataggt atccatttta gtttggcgcc tttttggtac 7500
cctccatttt gccttgcaac cggtttcggt tgtgcatatc ttgtaaatac cacaagcata 7560
ttcagcagaa ctgttaatcc tttggcatag tgccgtttcc tgtgtttaat gtttttttgt 7620
tttatacact atgccttacc tattagtcac ttactgtagg ctgccaacta tgcttttacc 7680
tgcatacctt agtgcttttg ggcacacatt tttattgcaa acatctgcac tttagtgttt 7740
tggcttgcag catacgttct gctagccaag tatctgtcta gtaaaccagg tgtgcaccta 7800
ttactcatgc accgcaaccg tttacggttt tgcagcaaca ggcctttttt ataattatt 7859
<210> 11
<211> 7845
<212> DNA
<213> Artificial Sequence
<220>
<223> Artificial Synthesis
<400> 11
gaaagtttca atcatacttt tatatattgg gagtgaccga aaagggttta agaccgaaaa 60
cggtacatat aaaaggcagc ttattctgtg tggacatatc catggagcca caattcaaca 120
atccacagga acgtccacga agcctgcacc acttgagtga ggtattagaa atacctttaa 180
ttgatcttag attatcatgt gtatattgca aaaaagaact aacacgtgct gaggtatata 240
attttgcatg cactgaatta aaattagtgt atagggatga ttttccttat gcagtgtgca 300
gagtatgttt attgttttat agtaaagtta gaaaatatag gtattatgac tattcagtgt 360
atggagctac actagaaagt ataactaaaa aacagttatg tgatttatta ataaggtgct 420
acagatgtca aagtccgtta actccggagg aaaagcaatt gcattgtgac agaaaaagac 480
gatttcatct aatagcacat ggttggaccg ggtcatgttt ggggtgctgg agacaaacat 540
ctagagaacc tagagaatct acagtataat catgcatggt aaagtaccaa cgctgcaaga 600
cgttgtatta gaactaacac ctcaaacaga aattgaccta cagtgcaatg agcaattgga 660
cagctcagag gatgaggatg aggatgaagt agaccatttg caggagcggc cacagcaagc 720
tagacaagct aaacaacata cgtgttacct aatacacgta ccttgttgtg agtgtaagtt 780
tgtggtgcag ttggacattc agagtaccaa agaggacctg cgtgttgtac aacagctgct 840
tatgggtgcg ttaacagtaa cgtgcccact ctgcgcatca agtaactaac tgcaatggcg 900
tcacctgaag gtacagatgg ggaggggaag ggatgttgtg gatggtttga agtagaggca 960
attgtagaaa aaaaaacagg agataaaata tcagatgatg aaagtgacga ggaggatgaa 1020
atagatacag atttagatgg atttatagac gattcatata tacaaaatat acaggcagac 1080
gcagaaacag ctcaacaatt gttgcaagta caaacagcac atgcagataa acagacgttg 1140
caaaaactaa aacgaaagta tatagctagt ccattaaggg atattagtaa tcagcaaact 1200
gtgtgccggg aaggagtaaa acggaggctt attttatcag acctacaaga cagcgggtat 1260
ggcaatacat tggaaactct ggaaacacca gaacaggtag atgaagaggt acagggacgt 1320
gggtgcggga atacacaaaa tggaggctca caaaacagta cctatagtaa caatagtgag 1380
gactctgtaa tacatatgga tattgataga aacaatgaaa cgccaacaca acaattgcag 1440
gacttgttta aaagtagcaa tttacaaggt aaattatatt ataaatttaa agaagtgtat 1500
ggtattccat tttcagaatt ggtgcgtacg tttaaaagtg atagtacatg ttgcaatgat 1560
tggatatgtg ctatatttgg tgttaatgaa acattagccg aggcactaaa aactataata 1620
aaaccacact gtatgtatta tcatatgcaa tgtttaacat gtacatgggg ggttatagta 1680
atgatgctaa ttagatatac atgtggcaaa aacagaaaaa caattgcaaa agcattaagc 1740
tcaatattaa atgtaccaca ggagcaaatg ttaattcaac caccaaaaat acgaagtcct 1800
gctgtagctt tatattttta taaaacagca atgtcaaata ttagtgatgt gtatggagac 1860
acaccagaat ggatacaaag acaaacacaa ttgcaacaca gtttacagga tagtcaattt 1920
gaattatcta aaatggtgca gtgggcattt gataatgaag taacagatga tagccaaatt 1980
gcgtttcaat atgcacaatt agcagatgta gacagcaatg cacaagcctt tttaaaaagc 2040
aatatgcagg caaaatatgt aaaggattgt ggaataatgt gtagacatta taaaagggca 2100
caacagcaac aaatgaatat gtgccagtgg ataaagcaca tatgtagtaa aacagatgaa 2160
gggggtgatt ggaaacccat tgtacaattt ttaagatatc aaggggtcga tttcatttca 2220
tttctaagtt actttaaatt atttctacaa ggaacaccta aacataactg tttggtactt 2280
tgtggaccgc caaatacagg taaatcatgc tttgctatga gtcttataaa gttttttcaa 2340
gggtctgtca tttcatttgt gaattcacaa agccactttt ggttgcagcc attagacaat 2400
gctaaacttg ggttgttgga tgatgcaaca gaaatatgtt ggaaatatat agacgattat 2460
ttaaggaatt tggtagatgg aaatcctata agtttagata gaaaacataa acaattagta 2520
caaataaaat gtccaccatt actaattaca accaatataa atcctatgct agatgctaaa 2580
ttacgatatt tacacagtag aatgttagtg tttcagtttc aaaatccatt tccattagat 2640
aataatggta atcctgtata tgaattaagt aatgtaaact ggaaatgttt ctttacaagg 2700
acgtggtcca gattaaattt ggataacgac gaggacaaag aaaacaatgg agacgctttc 2760
ccaacgttta aatgcgtgcc agaacaaaat actagactgt tttgaaaaag atagtagatg 2820
tattgcagat catatagaat attggaaagc tgtgcgacat gaaaatgtgc tatactataa 2880
agcaagagaa aatgacatta ctgtactaaa ccaccagatg gtgccttgtt tacaagtatg 2940
taaagcaaaa gcatgtagtg caatagaagt gcaaatagca ctggaatcat taagtacaac 3000
aatatataac aatgaagagt ggacattaag agacacatgc gaggaactat ggcttactga 3060
acctaaaaaa tgctttaaaa aagaaggaca acatatagaa gtatggtttg atggtagtaa 3120
aaacaattgt atgcaatatg tagcctggaa atatatatat tacaatggag attgtgggtg 3180
gcaaaaagtg tgttctgggg tagactatag aggtatatat tatgtacatg atggccacaa 3240
aacatactac acagactttg aacaagaggc caaaaaattt gggtgtaaaa acatatggga 3300
agtacatatg gaaaatgaga gtatttattg tcctgactct gtgtctagta cctgtagata 3360
caacgtatcc cctgttgaaa ctgttaacga atacaacacc cacaagacca ccaccaccac 3420
ctccacgtcc gtgggcaacc aagacgccgc agtatcccac agaccaggaa aacgacccag 3480
actacgggaa tcagaatttg actcctccag agagtcccac gcaaagtgtg tcacaacaca 3540
cacacacatc agcgacacag acaataccga cagtagaagt agaagtatca acaacaacaa 3600
ccaccctggt gataagacta cgcctgtagt acatttaaaa ggtgaaccta acagattaaa 3660
atgttgtaga tatcgatttc aaaaatataa aacattgttt gtggatgtaa catcaacata 3720
tcattggaca agtacagaca ataaaaatta tagcataatt acaattatat ataaggatga 3780
aacacaacga aacagctttt taagtcatgt aaaaattccc agtagtgtac aggttagttt 3840
gggacaaatg agttttccat aaagtgctgt atatattgta tatacatttg tgttattgta 3900
acacacaaat acgtgaagtg tacctgccat acattgctgc tacgcatata tattgcaacc 3960
attgattttt gtgttattgg tgtgtttgcg ctttgctttt gtgtttgttt gcttgtgtgt 4020
catgttgtcc cgcttttgct atctgcctct gtgttttcca gttgtatatt attaataata 4080
ttgttttggt ttgttatagc cacatccttt tttaatacat ttataatatt tttgatattt 4140
ttttactgtc ctgtgctgtg tatatattta catgctttgt ggataataaa taatatgtaa 4200
atgtagtagt actgttacta ctatggttgc ccaccgtgcc acacgacgca aacgcgcatc 4260
tgcaacacaa ctatataaaa catgtaagtt gtctggtaca tgtccagagg atgttgttaa 4320
taaaatagag caaaaaacat gggctgataa aatattgcaa tggggaagtt tatttacata 4380
ttttggaggc cttggcattg gtacaggaac tgggtctggg ggtcgtgcag gctatgttcc 4440
attggggtct aggccttcca caatagttga tgtaactccg gcgcgaccac ctattgttgt 4500
ggaatccgta gggcctacag acccttccat tgttacatta gttgaggagt ccagtgttat 4560
agaatctggt gcagggattc ctaattttac tgggtctggg ggatttgaaa ttacatcctc 4620
atcaacaact acacctgccg tgttggatat tacaccaacc tctagtactg tacatgtcag 4680
tagtacccat ataaccaatc cgttatttat tgatccccct gttattgagg ccccacaaac 4740
aggcgaggtg tctggcaata ttttaattag cacacccaca tctggtatac atagctatga 4800
agaaatacct atgcaaacat ttgctgttca cggttctggt acagaaccta ttagtagtac 4860
tcctattcca ggctttaggc gtattgcagc tcctagatta tatagaaaag catttcagca 4920
ggttaaggta actgaccctg catttcttga tagacctgca acattagtat ctgctgataa 4980
tccacttttt gaaggtactg acacatcttt agctttttct ccgtcgggtg tggctcctga 5040
ccctgatttt atgaatatag tagcattaca taggcctgca tttactacac gtaggggtgg 5100
tgtacgtttt agtaggcttg gcagaaaggc tactatacaa acacgtagag gcacacaaat 5160
aggtgcccgt gtgcattatt attatgatat aagtcctatt gcacaggctg aggaaattga 5220
aatgcagcca ttattgtctg caaataattc atttgatggc ctatatgata tttatgcaaa 5280
tatagatgat gaagcacctg gtttgtctag ccagtcagtt gctacacctt ctgcacactt 5340
acctataaag ccttccacat tgtcttttgc tagtaacacc actaatgtaa ctgccccttt 5400
aggtaatgtg tgggaaacac cattttattc aggtcctgac atagtgttgc ctacaggccc 5460
cagtacgtgg ccctttgttc ctcagtctcc ttatgatgtt acccatgatg tatatataca 5520
gggatcctcc tttgcattat ggcctgtgta tttttttaga cgtaggcgcc gtaaacgtat 5580
tccctatttt tttgcagatg gcgacgtggc ggcctagtga aaataaggtg tatctacctc 5640
caacacctgt ttcaaaggtt gtggcaacgg attcctatgt aaaacgcact agtatatttt 5700
atcatgcagg cagttcacga ttgcttgccg taggacatcc ctattactct gtgactaagg 5760
acaataccaa aacaaacatt cccaaagtta gtgcatatca atatagggta tttagggtac 5820
ggttgcccga ccctaataag tttgggcttc cagatactaa tatttataat ccggaccagg 5880
aacggttagt gtgggcatgt gtaggtttgg aggtaggccg cggacagcct ttaggtgctg 5940
ggctaagtgg ccatccattg tttaataggc tggatgatac tgaaagttcc aatttagcaa 6000
ataataatgt tatagaagat agtagggaca atatatcagt tgatggcaag caaacacagt 6060
tgtgtattgt tggatgtact cccgctatgg gtgaacattg gactaaaggt gctgtgtgta 6120
agtccacaca agttaccaca ggggactgcc cgcctcttgc attaattaat acacctatag 6180
aggatgggga catgatagac acaggatttg gcgctatgga ctttaaggtg ttgcaggaat 6240
ctaaggctga ggtaccttta gacattgtac aatccacctg taaatatcct gactatttaa 6300
aaatgtctgc agatgcctat ggtgattcta tgtggtttta cttacgcagg gaacaattat 6360
ttgccagaca ttattttaat agggctggta aagttgggga aacaatacct gcagagttat 6420
atttaaaggg tagcaatggt agagaacccc ctccgagttc tgtatatgtt gctacgccta 6480
gtgggtctat gattacgtct gaggcacagt tatttaataa accttattgg ttgcaacgtg 6540
cccaaggcca taataatggc atttgctggg gtaatcaatt atttgttact gtagtagata 6600
ctactagaag tactaacatg actattagta ctgctacaga acagttaagt aaatatgatg 6660
cacgaaaaat taatcagtac cttagacatg tggaggaata tgaattacaa tttgtttttc 6720
aattatgcaa aattactttg tctgcagagg ttatggcata tttacataat atgaatgcta 6780
acctactgga ggactggaat attgggttat ccccgccagt ggccaccagc ctagaagata 6840
aatatagata tgttagaagc acagctataa catgtcaacg ggaacagcca ccaacagaaa 6900
aacaggaccc attagctaaa tataaatttt gggatgttaa cttacaggac agtttttcta 6960
cagacctgga tcaatttcca ctgggtagaa aatttttaat gcaactgggc actaggtcaa 7020
agcctgctgt agctacctct aaaaagcgat ctgctcctac ctccacctct acaccagcaa 7080
aacgtaaaag gcggtagtgt gttgttgtgt gtttgtgtaa ctgtgtttgt gtgttgtata 7140
tatggtatgt ttgtgtatgt gctttatttt atactttgta tgtgtatgtt gtgtttgtgt 7200
aaatgtttgt gtgaaatgtt tgtgtgtgta ttcattgtat gtatgactgt atatatgtgt 7260
aatgtttgtg tgtctgtaat aaacatgaat gagtgctttt acgcgtggtt gcataaacta 7320
aggtgtgtca ttattgtggc ttttgttttg taagttattg tgtacagtgt actatgtgta 7380
ttgtgcatac atatatatac cataacatac tccattttgt tgtttttccg ccattttgta 7440
catgcaaccg aattcggttg catggcctag tgccattatt taaactaaaa ggaattcggt 7500
tgcatggcct agtgccatta tttaaaccaa aaggcccttt tcagcagaac agttaatcct 7560
ttggcatatt gccgtttcct gtgttttata cttgaattat gtacagtacc gcaccctgta 7620
ttactcacag gtactatgac tgccaactat gcttttatct gcatacttta gtgctgttgg 7680
gcacacattt ttatacatgt gtctgcaact ttggtgtttt ggcttgcaga atacactatg 7740
taggccaagt atctgtcagt atctgttttg caaacatgta acatacaatt actcattttt 7800
taaaaccgtt tacggtcgtg caaaaacagg tttcttttaa ttgtt 7845
<210> 12
<211> 7824
<212> DNA
<213> Artificial Sequence
<220>
<223> Artificial Synthesis
<400> 12
ctaaactata atgccaaatc ttgtaaaaac tagggtgtaa ccgaaaacgg tctgaccgaa 60
accggtgcat atataaagca gacatttttt ggtaggctac tgcaggacta tgttccagga 120
cgcagaggag aaaccacgga cattgcatga tttgtgtcag gcgttggaga catctgtgca 180
tgaaatcgaa ttgaaatgcg ttgaatgcaa aaagactttg cagcgatctg aggtatatga 240
ctttgtattt gcagatttaa gaatagtgta tagagatgga aatccatttg cagtatgtaa 300
agtgtgctta cgattgctat ctaaaataag tgagtataga cattataatt attcgctata 360
tggagacaca ttagaacaaa cactaaaaaa gtgtttaaat gaaatattaa ttagatgtat 420
tatttgtcaa agaccattgt gtccacaaga aaaaaaaagg catgtggatt taaacaaaag 480
gtttcataat atttcgggtc gttggacagg gcgctgtgca gtgtgttgga gaccccgacg 540
tagacaaaca caagtgtaac ctgtaacaac gccatgagag gaaacaaccc aacgctaaga 600
gaatatattt tagatttaca tcctgaacca actgacctat tctgctatga gcaattatgt 660
gacagctcag acgaggatga aataggcttg gacgggccag atggacaagc acaaccggcc 720
acagctaatt actacattgt aacttgttgt tacacttgtg gcaccacggt tcgtttgtgt 780
atcaacagta caacaaccga cgtacgaacc ctacagcagc tgcttatggg cacatgtacc 840
attgtgtgcc ctagctgtgc acagcaataa acaccatctg caatggatga ccctgaaggt 900
acaaacgggg taggggcggg ctgtactggc tggtttgagg tagaagcggt aatagaacga 960
agaacaggag ataatatttc agatgatgag gacgaaacag cagacgatag tggtacagat 1020
ttaatagagt ttatagatga ttcagtacaa agtactacac aggcagaagc agaggcagcc 1080
cgagcgttgt ttaatgtaca ggaaggggtg gacgatataa atgctgtgtg tgcactaaaa 1140
cgaaagtttg cagcatgctc agaaagtgct gtagaggact gtgtggaccg ggctgcaaat 1200
gtgtgtgtat cgtggaaata taaaaataaa gaatgcacac acagaaaacg aaaaattatt 1260
gagctagaag acagcggata tggcaatact gaagtggaaa ctgagcagat ggcacaccag 1320
gtagaaagcc aaaatggcga cgcagactta aatgactcgg agtctagtgg ggtgggggct 1380
agttcagatg taagcagtga aacggatgta gacagttgta atactgttcc attacaaaat 1440
attagtaata ttctacataa cagtaatact aaagcaacgc tattatataa attcaaagaa 1500
gcttatggag taagttttat ggaattagtt agaccattta aaagtgataa aacaagctgt 1560
acagattggt gtataacagg gtatggaata agtccctccg tagcagaaag tttaaaagta 1620
ctaattaaac agcacagtat atatacacac ctacaatgtt taacgtgtga cagaggaatt 1680
atattattat tgttaattag atttaaatgt agcaaaaata gattaactgt ggcaaaatta 1740
atgagtaatt tactatcaat tcctgaaaca tgtatgatta tcgagccacc aaaattacga 1800
agtcaagcat gtgccttata ttggtttaga acagcaatgt caaatataag tgatgtgcaa 1860
gggacaacac cagaatggat agatagatta acagtgttac agcatagctt taatgatgat 1920
atatttgatt taagtgaaat gatacaatgg gcatatgata atgacattac agatgatagt 1980
gacattgcat ataaatatgc acagttagca gatgttaata gtaatgcagc agcattttta 2040
agaagcaatg cacaagcaaa aatagtaaaa gactgtggcg ttatgtgcag acattataaa 2100
agagcagaaa agcgtggtat gacaatggga caatggatac aaagtaggtg tgaaaaaaca 2160
aatgatggag gtaattggag accaatagta caatttttaa gatatcaaaa tattgaattt 2220
acagcatttt tagttgcatt taaacagttt ttacaaggtg taccaaaaaa aagttgtatg 2280
ttactgtgtg gcccagcaaa tacagggaaa tcatattttg gaatgagttt aatacatttt 2340
ttaaaaggat gcattatttc atatgtaaat tccaaaagtc atttttggtt gcagccatta 2400
tcagatgcta aactaggtat gatagatgat gtaacagcca taagctggac atatatagat 2460
gattatatga gaaatgcatt agatggtaac gacatttcaa tagatgtaaa acatagggca 2520
ttagtacaat taaaatgtcc accattaata attacctcaa atacaaatgc aggcaaagat 2580
tcacgatggc catatttgca cagtagacta acagtatttg aatttaacaa tccatttcca 2640
tttgatgcaa atggtaatcc agtgtataaa ataaatgatg aaaattggaa atcctttttc 2700
tcaaggacgt ggtgcaaatt aggcttaata gaggaagagg acaaggaaaa cgatggagga 2760
aatatcagca cgtttaagtg cagtgcagga caaaatccta gacatatacg aagctgataa 2820
aaatgattta acatcacaaa ttgaacattg gaaactaata cgcatggagt gtgctataat 2880
gtatacagcc agacaaatgg gaatatcaca tttgtgccac caggtggtgc cgtcattggt 2940
agcatcaaag actaaagcgt ttcaagtaat tgaactgcaa atggcattag agacattaaa 3000
tgcatcacca tataaaacag atgaatggac attgcaacaa acaagcttag aagtgtggtt 3060
atcagagcca caaaaatgct ttaaaaaaaa aggcataaca gtaactgtac aatatgacaa 3120
tgataaagca aacacaatgg attatacaaa ttggagtgaa atatatatta ttgaggaaac 3180
aacatgtact ttggtagcag gagaagttga ctatgtgggg ttgtattata tacatggcaa 3240
tgaaaagacg tattttaaat attttaaaga ggatgcaaaa aagtactcta aaacacaatt 3300
atgggaggta catgtgggta gtcgggtaat tgtatgtcct acatctatac ctagtgatca 3360
aatatccact actgaaactg ctgacccaaa gaccaccgag gccaccaaca acgaaagtac 3420
acaggggaca aagcgacgac gactcgattt accagactcc agagacaaca cccagtactc 3480
cacaaagtat acagactgcg ccgtggacag tagaccacga ggaggaggac tacacagtac 3540
aactaactgt acatacaaag ggcggaacgt gtgtagttct aaagtttcac ctatcgtgca 3600
tttaaaaggt gacccaaata gtttaaaatg tttaagatat agattaaaac catttaaaga 3660
cttatactgt aatatgtcat ccacatggca ttggaccagt gatgacaaag gtgacaaagt 3720
aggaattgtt actgtaacat acacaacgga aacacaacga caactgtttt taaacactgt 3780
taaaatacca cccactgtgc aaataagtac tggtgttatg tcattgtaat tgtattgtac 3840
aattactgta tgtaaaccac aagccaatat gtgctgctaa gtgtatatac aatgatatta 3900
cctatttttg ttgtttgttt tatactgttt ttatgcttgt gcattttttt gcggccattg 3960
gtgctatcta tttctatata tgcttggttg ctggtgttgg tgttgctgct ttgggtgtct 4020
gtggggtcgg ctctacgaat ttttttctgt tacttaatat ttttatatat accaatgatg 4080
tgtattaatt ttcatgcaca atacttaacc caacaagact aactgtatac tggttctgca 4140
catggtggta tggtattgta aatatttact gttgtgtgtg ttgtttttat tatttttata 4200
catttactaa taaatacttt tatattttta gcactgtctt attatgagac acaaacggtc 4260
tacaaggcgc aagcgtgcat ctgctacaca actttaccaa acatgcaagg cctcaggcac 4320
ctgcccacct gatgttatac ccaaagttga aggcactact atagcagatc aaatattacg 4380
atatggtagc ttaggggtgt tttttggagg tttaggcatt ggtacagggt cgggtacagg 4440
tggcaggact ggatatgtgc cccttggtag taccccaccg tctgaggcta tacctttaca 4500
gcccatacgt cccccagtta ccgttgatac tgtggggcct ttggattctt ctattgtatc 4560
tttaatagag gaatctagtt ttatagacgc cggtgcacca gccccatcaa ttcccactcc 4620
atctggtttt gatattacca cctctgcaga tactacacct gcaatactta atgtttcctc 4680
tattggagaa tcatctatac aaactgtttc tacacattta aatccctcct ttactgagcc 4740
atccgtactc cgccctcctg cacctgcaga ggcctctgga catttaatat tttcctctcc 4800
tactgttagc acacatagtt atgaaaacat accaatggat acctttgtta tttctactga 4860
cagtggcaat gtcacgtcta gcacacccat tccagggtct cgccctgtgg cacgccttgg 4920
tttatacagt cgcaacaccc aacaagttaa ggttgttgac cctgcttttt taacatctcc 4980
tcatagactt gtaacatatg ataatccagc atttgaaggc tttaaccctg aggacacatt 5040
gcagtttcaa catagtgaca tatcgcctgc tcctgatcct gattttctag atattgttgc 5100
attacacaga cctgcattaa cctctcgcag gggtactgta cgttatagta gggttgggca 5160
aaaggctaca cttcgtactc gcagtggaaa gcaaataggg gctaaagtac attactacca 5220
agacttaagt cccatacagc ctgtccagga acaggtacaa cagcagcaac aatttgaatt 5280
acaatcttta aatacttctg tttctcccta tagtattaat gatggacttt atgatattta 5340
tgctgacgat gctgatacta tacatgattt tcagagtcct ctgcactcac atacgtcctt 5400
tgccaccaca cgtaccagta atgtgtccat accattaaat actggatttg acactcctct 5460
tgtgtcattg gaacctggtc cagacattgc atcttctgta acatctatgt ctagtccatt 5520
tattcctata tctccactaa ctccttttaa taccataatt gtggatggtg ctgattttat 5580
gttgcaccct agctatttta ttttgcgtcg cagacgtaaa cgttttccat atttttttgc 5640
agatgtccgt gtggcggcct agtgaggcca ctgtgtacct gcctcctgtg cctgtgtcta 5700
aggttgtaag cactgatgaa tatgtgtcac gcacaagcat ttattattat gctggcagtt 5760
ccagactttt ggctgttggc aatccatatt tttccatcaa aagtcccaat aacaataaaa 5820
aagtattagt tcccaaggta tcaggcttac agtatagggt ctttagggtg cgtttacctg 5880
atcccaataa atttggtttt cctgatacat ctttttataa ccctgataca caacgtttgg 5940
tctgggcatg tgtaggcctt gaaataggta ggggacagcc attgggtgtt ggcgtaagtg 6000
gtcatcctta tttaaataaa tttgatgaca ctgaaaccag taacagatat cccgcacagc 6060
cagggtctga taacagggaa tgcttatcta tggattataa acaaacacaa ttatgtttaa 6120
ttggctgtaa acctcccact ggtgagcatt ggggtaaagg tgttgcctgt aacaataatg 6180
cagctgctac tgattgtcct ccattggaac tttttaattc tattattgag gatggtgaca 6240
tggtagatac agggtttgga tgcatggact ttggtacatt gcaggctaat aaaagtgatg 6300
tgcctattga tatttgtaac agtacatgca aatatccaga ttatttaaaa atggccagtg 6360
aaccttatgg ggatagtttg ttcttttttc ttagacgtga gcagatgttt gttagacact 6420
tttttaatag ggctggaaaa cttggcgagg ctgtcccgga tgacctttat attaaagggt 6480
ccggtaatac tgcagttatc caaagtagtg cattttttcc aactcctagt ggctctatag 6540
ttacctcaga atcacaatta tttaataagc cttattggct acagcgtgca caaggtcata 6600
acaatggcat ttgctggggc aatcagttat ttgttaccgt ggttgatacc actcgtagca 6660
ctaatatgac attatgcact gaagtaacta aggaaggtac atataaaaat gataatttta 6720
aggaatatgt acgtcatgtt gaagaatatg acttacagtt tgtttttcag ctttgcaaaa 6780
ttacactaac tgcagagata atgacatata tacatactat ggattccaat attttggagg 6840
actggcaatt tggtttaaca cctcctccgt ctgccagttt acaggacaca tatagatttg 6900
ttacctccca ggctattact tgccaaaaaa cagcaccccc taaagaaaag gaagatccat 6960
taaataaata tactttttgg gaggttaact taaaggaaaa gttttctgca gatctagatc 7020
agtttccttt gggacgaaag tttttattac aatcaggcct taaagcaaag cccagactaa 7080
aacgttcggc ccctactacc cgtgcaccat ccaccaaacg caaaaaggtt aaaaaataat 7140
tgttgtggta cttacactat tttattatac atgtttgttt gttttatgta tgtgttgtct 7200
gtttgtttat gtttgtgtat atgttgtatg tgttatgtgt catgtttgtg tacatgttct 7260
atgtccttgt cagtttcctg tttctgtata tatgtaataa actattgtgt gtattgtaaa 7320
ctatttgtat tgtttgggtg tatctatgag taaggtgctg tccctaaatt gccctaccct 7380
gccctgccta ttatgcatac ctatgtaata gtatttgtat gatatgtatt ttatagtttt 7440
taacagtact gcctccattt tactttacct ccattttgtg catgtaaccg atttcggttg 7500
ctggcacaaa cgtgtttttt ttaaactaca atttaaacaa tacagttaat cctttccctt 7560
cctgcactgc ttttgcctat acttgcatat gtgactcata tatacatgca gtgcagttgc 7620
aaaatgttta attatactca tagtttaaac atgcttatag gcacatattt taacttactt 7680
tcaatgctta agtgcagttt tggcttgcac aatagtttgt tatgccaaac tatgtcttgt 7740
aaaagtgact cactaacatt tattgccagg tgtggactaa ccgttttggg tcacattgtt 7800
catgtttcaa cattttatat aata 7824
<210> 13
<211> 7824
<212> DNA
<213> Artificial Sequence
<220>
<223> Artificial Synthesis
<400> 13
ctaaactata atgccaaatc ttgtaaaaac tagggtgtaa ccgaaaacgg tctgaccgaa 60
accggtgcat atataaagca gacatttttt ggtaggctac tgcaggacta tgttccagga 120
cgcagaggag aaaccacgga cattgcatga tttgtgtcag gcgttggaga catctgtgca 180
tgaaatcgaa ttgaaatgcg ttgaatgcaa aaagactttg cagcgatctg aggtatatga 240
ctttgtattt gcagatttaa gaatagtgta tagagatgga aatccatttg cagtatgtaa 300
agtgtgctta cgattgctat ctaaaataag tgagtataga cattataatt attcgctata 360
tggagacaca ttagaacaaa cactaaaaaa gtgtttaaat gaaatattaa ttagatgtat 420
tatttgtcaa agaccattgt gtccacaaga aaaaaaaagg catgtggatt taaacaaaag 480
gtttcataat atttcgggtc gttggacagg gcgctgtgca gtgtgttgga gaccccgacg 540
tagacaaaca caagtgtaac ctgtaacaac gccatgagag gaaacaaccc aacgctaaga 600
gaatatattt tagatttaca tcctgaacca actgacctat tctgctatga gcaattatgt 660
gacagctcag acgaggatga aataggcttg gacgggccag atggacaagc acaaccggcc 720
acagctaatt actacattgt aacttgttgt tacacttgtg gcaccacggt tcgtttgtgt 780
atcaacagta caacaaccga cgtacgaacc ctacagcagc tgcttatggg cacatgtacc 840
attgtgtgcc ctagctgtgc acagcaataa acaccatctg caatggatga ccctgaaggt 900
acaaacgggg taggggcggg ctgtactggc tggtttgagg tagaagcggt aatagaacga 960
agaacaggag ataatatttc agatgatgag gacgaaacag cagacgatag tggtacagat 1020
ttaatagagt ttatagatga ttcagtacaa agtactacac aggcagaagc agaggcagcc 1080
cgagcgttgt ttaatgtaca ggaaggggtg gacgatataa atgctgtgtg tgcactaaaa 1140
cgaaagtttg cagcatgctc agaaagtgct gtagaggact gtgtggaccg ggctgcaaat 1200
gtgtgtgtat cgtggaaata taaaaataaa gaatgcacac acagaaaacg aaaaattatt 1260
gagctagaag acagcggata tggcaatact gaagtggaaa ctgagcagat ggcacaccag 1320
gtagaaagcc aaaatggcga cgcagactta aatgactcgg agtctagtgg ggtgggggct 1380
agttcagatg taagcagtga aacggatgta gacagttgta atactgttcc attacaaaat 1440
attagtaata ttctacataa cagtaatact aaagcaacgc tattatataa attcaaagaa 1500
gcttatggag taagttttat ggaattagtt agaccattta aaagtgataa aacaagctgt 1560
acagattggt gtataacagg gtatggaata agtccctccg tagcagaaag tttaaaagta 1620
ctaattaaac agcacagtat atatacacac ctacaatgtt taacgtgtga cagaggaatt 1680
atattattat tgttaattag atttaaatgt agcaaaaata gattaactgt ggcaaaatta 1740
atgagtaatt tactatcaat tcctgaaaca tgtatgatta tcgagccacc aaaattacga 1800
agtcaagcat gtgccttata ttggtttaga acagcaatgt caaatataag tgatgtgcaa 1860
gggacaacac cagaatggat agatagatta acagtgttac agcatagctt taatgatgat 1920
atatttgatt taagtgaaat gatacaatgg gcatatgata atgacattac agatgatagt 1980
gacattgcat ataaatatgc acagttagca gatgttaata gtaatgcagc agcattttta 2040
agaagcaatg cacaagcaaa aatagtaaaa gactgtggcg ttatgtgcag acattataaa 2100
agagcagaaa agcgtggtat gacaatggga caatggatac aaagtaggtg tgaaaaaaca 2160
aatgatggag gtaattggag accaatagta caatttttaa gatatcaaaa tattgaattt 2220
acagcatttt tagttgcatt taaacagttt ttacaaggtg taccaaaaaa aagttgtatg 2280
ttactgtgtg gcccagcaaa tacagggaaa tcatattttg gaatgagttt aatacatttt 2340
ttaaaaggat gcattatttc atatgtaaat tccaaaagtc atttttggtt gcagccatta 2400
tcagatgcta aactaggtat gatagatgat gtaacagcca taagctggac atatatagat 2460
gattatatga gaaatgcatt agatggtaac gacatttcaa tagatgtaaa acatagggca 2520
ttagtacaat taaaatgtcc accattaata attacctcaa atacaaatgc aggcaaagat 2580
tcacgatggc catatttgca cagtagacta acagtatttg aatttaacaa tccatttcca 2640
tttgatgcaa atggtaatcc agtgtataaa ataaatgatg aaaattggaa atcctttttc 2700
tcaaggacgt ggtgcaaatt aggcttaata gaggaagagg acaaggaaaa cgatggagga 2760
aatatcagca cgtttaagtg cagtgcagga caaaatccta gacatatacg aagctgataa 2820
aaatgattta acatcacaaa ttgaacattg gaaactaata cgcatggagt gtgctataat 2880
gtatacagcc agacaaatgg gaatatcaca tttgtgccac caggtggtgc cgtcattggt 2940
agcatcaaag actaaagcgt ttcaagtaat tgaactgcaa atggcattag agacattaaa 3000
tgcatcacca tataaaacag atgaatggac attgcaacaa acaagcttag aagtgtggtt 3060
atcagagcca caaaaatgct ttaaaaaaaa aggcataaca gtaactgtac aatatgacaa 3120
tgataaagca aacacaatgg attatacaaa ttggagtgaa atatatatta ttgaggaaac 3180
aacatgtact ttggtagcag gagaagttga ctatgtgggg ttgtattata tacatggcaa 3240
tgaaaagacg tattttaaat attttaaaga ggatgcaaaa aagtactcta aaacacaatt 3300
atgggaggta catgtgggta gtcgggtaat tgtatgtcct acatctatac ctagtgatca 3360
aatatccact actgaaactg ctgacccaaa gaccaccgag gccaccaaca acgaaagtac 3420
acaggggaca aagcgacgac gactcgattt accagactcc agagacaaca cccagtactc 3480
cacaaagtat acagactgcg ccgtggacag tagaccacga ggaggaggac tacacagtac 3540
aactaactgt acatacaaag ggcggaacgt gtgtagttct aaagtttcac ctatcgtgca 3600
tttaaaaggt gacccaaata gtttaaaatg tttaagatat agattaaaac catttaaaga 3660
cttatactgt aatatgtcat ccacatggca ttggaccagt gatgacaaag gtgacaaagt 3720
aggaattgtt actgtaacat acacaacgga aacacaacga caactgtttt taaacactgt 3780
taaaatacca cccactgtgc aaataagtac tggtgttatg tcattgtaat tgtattgtac 3840
aattactgta tgtaaaccac aagccaatat gtgctgctaa gtgtatatac aatgatatta 3900
cctatttttg ttgtttgttt tatactgttt ttatgcttgt gcattttttt gcggccattg 3960
gtgctatcta tttctatata tgcttggttg ctggtgttgg tgttgctgct ttgggtgtct 4020
gtggggtcgg ctctacgaat ttttttctgt tacttaatat ttttatatat accaatgatg 4080
tgtattaatt ttcatgcaca atacttaacc caacaagact aactgtatac tggttctgca 4140
catggtggta tggtattgta aatatttact gttgtgtgtg ttgtttttat tatttttata 4200
catttactaa taaatacttt tatattttta gcactgtctt attatgagac acaaacggtc 4260
tacaaggcgc aagcgtgcat ctgctacaca actttaccaa acatgcaagg cctcaggcac 4320
ctgcccacct gatgttatac ccaaagttga aggcactact atagcagatc aaatattacg 4380
atatggtagc ttaggggtgt tttttggagg tttaggcatt ggtacagggt cgggtacagg 4440
tggcaggact ggatatgtgc cccttggtag taccccaccg tctgaggcta tacctttaca 4500
gcccatacgt cccccagtta ccgttgatac tgtggggcct ttggattctt ctattgtatc 4560
tttaatagag gaatctagtt ttatagacgc cggtgcacca gccccatcaa ttcccactcc 4620
atctggtttt gatattacca cctctgcaga tactacacct gcaatactta atgtttcctc 4680
tattggagaa tcatctatac aaactgtttc tacacattta aatccctcct ttactgagcc 4740
atccgtactc cgccctcctg cacctgcaga ggcctctgga catttaatat tttcctctcc 4800
tactgttagc acacatagtt atgaaaacat accaatggat acctttgtta tttctactga 4860
cagtggcaat gtcacgtcta gcacacccat tccagggtct cgccctgtgg cacgccttgg 4920
tttatacagt cgcaacaccc aacaagttaa ggttgttgac cctgcttttt taacatctcc 4980
tcatagactt gtaacatatg ataatccagc atttgaaggc tttaaccctg aggacacatt 5040
gcagtttcaa catagtgaca tatcgcctgc tcctgatcct gattttctag atattgttgc 5100
attacacaga cctgcattaa cctctcgcag gggtactgta cgttatagta gggttgggca 5160
aaaggctaca cttcgtactc gcagtggaaa gcaaataggg gctaaagtac attactacca 5220
agacttaagt cccatacagc ctgtccagga acaggtacaa cagcagcaac aatttgaatt 5280
acaatcttta aatacttctg tttctcccta tagtattaat gatggacttt atgatattta 5340
tgctgacgat gctgatacta tacatgattt tcagagtcct ctgcactcac atacgtcctt 5400
tgccaccaca cgtaccagta atgtgtccat accattaaat actggatttg acactcctct 5460
tgtgtcattg gaacctggtc cagacattgc atcttctgta acatctatgt ctagtccatt 5520
tattcctata tctccactaa ctccttttaa taccataatt gtggatggtg ctgattttat 5580
gttgcaccct agctatttta ttttgcgtcg cagacgtaaa cgttttccat atttttttgc 5640
agatgtccgt gtggcggcct agtgaggcca ctgtgtacct gcctcctgtg cctgtgtcta 5700
aggttgtaag cactgatgaa tatgtgtcac gcacaagcat ttattattat gctggcagtt 5760
ccagactttt ggctgttggc aatccatatt tttccatcaa aagtcccaat aacaataaaa 5820
aagtattagt tcccaaggta tcaggcttac agtatagggt ctttagggtg cgtttacctg 5880
atcccaataa atttggtttt cctgatacat ctttttataa ccctgataca caacgtttgg 5940
tctgggcatg tgtaggcctt gaaataggta ggggacagcc attgggtgtt ggcgtaagtg 6000
gtcatcctta tttaaataaa tttgatgaca ctgaaaccag taacagatat cccgcacagc 6060
cagggtctga taacagggaa tgcttatcta tggattataa acaaacacaa ttatgtttaa 6120
ttggctgtaa acctcccact ggtgagcatt ggggtaaagg tgttgcctgt aacaataatg 6180
cagctgctac tgattgtcct ccattggaac tttttaattc tattattgag gatggtgaca 6240
tggtagatac agggtttgga tgcatggact ttggtacatt gcaggctaat aaaagtgatg 6300
tgcctattga tatttgtaac agtacatgca aatatccaga ttatttaaaa atggccagtg 6360
aaccttatgg ggatagtttg ttcttttttc ttagacgtga gcagatgttt gttagacact 6420
tttttaatag ggctggaaaa cttggcgagg ctgtcccgga tgacctttat attaaagggt 6480
ccggtaatac tgcagttatc caaagtagtg cattttttcc aactcctagt ggctctatag 6540
ttacctcaga atcacaatta tttaataagc cttattggct acagcgtgca caaggtcata 6600
acaatggcat ttgctggggc aatcagttat ttgttaccgt ggttgatacc actcgtagca 6660
ctaatatgac attatgcact gaagtaacta aggaaggtac atataaaaat gataatttta 6720
aggaatatgt acgtcatgtt gaagaatatg acttacagtt tgtttttcag ctttgcaaaa 6780
ttacactaac tgcagagata atgacatata tacatactat ggattccaat attttggagg 6840
actggcaatt tggtttaaca cctcctccgt ctgccagttt acaggacaca tatagatttg 6900
ttacctccca ggctattact tgccaaaaaa cagcaccccc taaagaaaag gaagatccat 6960
taaataaata tactttttgg gaggttaact taaaggaaaa gttttctgca gatctagatc 7020
agtttccttt gggacgaaag tttttattac aatcaggcct taaagcaaag cccagactaa 7080
aacgttcggc ccctactacc cgtgcaccat ccaccaaacg caaaaaggtt aaaaaataat 7140
tgttgtggta cttacactat tttattatac atgtttgttt gttttatgta tgtgttgtct 7200
gtttgtttat gtttgtgtat atgttgtatg tgttatgtgt catgtttgtg tacatgttct 7260
atgtccttgt cagtttcctg tttctgtata tatgtaataa actattgtgt gtattgtaaa 7320
ctatttgtat tgtttgggtg tatctatgag taaggtgctg tccctaaatt gccctaccct 7380
gccctgccta ttatgcatac ctatgtaata gtatttgtat gatatgtatt ttatagtttt 7440
taacagtact gcctccattt tactttacct ccattttgtg catgtaaccg atttcggttg 7500
ctggcacaaa cgtgtttttt ttaaactaca atttaaacaa tacagttaat cctttccctt 7560
cctgcactgc ttttgcctat acttgcatat gtgactcata tatacatgca gtgcagttgc 7620
aaaatgttta attatactca tagtttaaac atgcttatag gcacatattt taacttactt 7680
tcaatgctta agtgcagttt tggcttgcac aatagtttgt tatgccaaac tatgtcttgt 7740
aaaagtgact cactaacatt tattgccagg tgtggactaa ccgttttggg tcacattgtt 7800
catgtttcaa cattttatat aata 7824
<210> 14
<211> 7824
<212> DNA
<213> Artificial Sequence
<220>
<223> Artificial Synthesis
<400> 14
ctaaactata atgccaaatc ttgtaaaaac tagggtgtaa ccgaaaacgg tctgaccgaa 60
accggtgcat atataaagca gacatttttt ggtaggctac tgcaggacta tgttccagga 120
cgcagaggag aaaccacgga cattgcatga tttgtgtcag gcgttggaga catctgtgca 180
tgaaatcgaa ttgaaatgcg ttgaatgcaa aaagactttg cagcgatctg aggtatatga 240
ctttgtattt gcagatttaa gaatagtgta tagagatgga aatccatttg cagtatgtaa 300
agtgtgctta cgattgctat ctaaaataag tgagtataga cattataatt attcgctata 360
tggagacaca ttagaacaaa cactaaaaaa gtgtttaaat gaaatattaa ttagatgtat 420
tatttgtcaa agaccattgt gtccacaaga aaaaaaaagg catgtggatt taaacaaaag 480
gtttcataat atttcgggtc gttggacagg gcgctgtgca gtgtgttgga gaccccgacg 540
tagacaaaca caagtgtaac ctgtaacaac gccatgagag gaaacaaccc aacgctaaga 600
gaatatattt tagatttaca tcctgaacca actgacctat tctgctatga gcaattatgt 660
gacagctcag acgaggatga aataggcttg gacgggccag atggacaagc acaaccggcc 720
acagctaatt actacattgt aacttgttgt tacacttgtg gcaccacggt tcgtttgtgt 780
atcaacagta caacaaccga cgtacgaacc ctacagcagc tgcttatggg cacatgtacc 840
attgtgtgcc ctagctgtgc acagcaataa acaccatctg caatggatga ccctgaaggt 900
acaaacgggg taggggcggg ctgtactggc tggtttgagg tagaagcggt aatagaacga 960
agaacaggag ataatatttc agatgatgag gacgaaacag cagacgatag tggtacagat 1020
ttaatagagt ttatagatga ttcagtacaa agtactacac aggcagaagc agaggcagcc 1080
cgagcgttgt ttaatgtaca ggaaggggtg gacgatataa atgctgtgtg tgcactaaaa 1140
cgaaagtttg cagcatgctc agaaagtgct gtagaggact gtgtggaccg ggctgcaaat 1200
gtgtgtgtat cgtggaaata taaaaataaa gaatgcacac acagaaaacg aaaaattatt 1260
gagctagaag acagcggata tggcaatact gaagtggaaa ctgagcagat ggcacaccag 1320
gtagaaagcc aaaatggcga cgcagactta aatgactcgg agtctagtgg ggtgggggct 1380
agttcagatg taagcagtga aacggatgta gacagttgta atactgttcc attacaaaat 1440
attagtaata ttctacataa cagtaatact aaagcaacgc tattatataa attcaaagaa 1500
gcttatggag taagttttat ggaattagtt agaccattta aaagtgataa aacaagctgt 1560
acagattggt gtataacagg gtatggaata agtccctccg tagcagaaag tttaaaagta 1620
ctaattaaac agcacagtat atatacacac ctacaatgtt taacgtgtga cagaggaatt 1680
atattattat tgttaattag atttaaatgt agcaaaaata gattaactgt ggcaaaatta 1740
atgagtaatt tactatcaat tcctgaaaca tgtatgatta tcgagccacc aaaattacga 1800
agtcaagcat gtgccttata ttggtttaga acagcaatgt caaatataag tgatgtgcaa 1860
gggacaacac cagaatggat agatagatta acagtgttac agcatagctt taatgatgat 1920
atatttgatt taagtgaaat gatacaatgg gcatatgata atgacattac agatgatagt 1980
gacattgcat ataaatatgc acagttagca gatgttaata gtaatgcagc agcattttta 2040
agaagcaatg cacaagcaaa aatagtaaaa gactgtggcg ttatgtgcag acattataaa 2100
agagcagaaa agcgtggtat gacaatggga caatggatac aaagtaggtg tgaaaaaaca 2160
aatgatggag gtaattggag accaatagta caatttttaa gatatcaaaa tattgaattt 2220
acagcatttt tagttgcatt taaacagttt ttacaaggtg taccaaaaaa aagttgtatg 2280
ttactgtgtg gcccagcaaa tacagggaaa tcatattttg gaatgagttt aatacatttt 2340
ttaaaaggat gcattatttc atatgtaaat tccaaaagtc atttttggtt gcagccatta 2400
tcagatgcta aactaggtat gatagatgat gtaacagcca taagctggac atatatagat 2460
gattatatga gaaatgcatt agatggtaac gacatttcaa tagatgtaaa acatagggca 2520
ttagtacaat taaaatgtcc accattaata attacctcaa atacaaatgc aggcaaagat 2580
tcacgatggc catatttgca cagtagacta acagtatttg aatttaacaa tccatttcca 2640
tttgatgcaa atggtaatcc agtgtataaa ataaatgatg aaaattggaa atcctttttc 2700
tcaaggacgt ggtgcaaatt aggcttaata gaggaagagg acaaggaaaa cgatggagga 2760
aatatcagca cgtttaagtg cagtgcagga caaaatccta gacatatacg aagctgataa 2820
aaatgattta acatcacaaa ttgaacattg gaaactaata cgcatggagt gtgctataat 2880
gtatacagcc agacaaatgg gaatatcaca tttgtgccac caggtggtgc cgtcattggt 2940
agcatcaaag actaaagcgt ttcaagtaat tgaactgcaa atggcattag agacattaaa 3000
tgcatcacca tataaaacag atgaatggac attgcaacaa acaagcttag aagtgtggtt 3060
atcagagcca caaaaatgct ttaaaaaaaa aggcataaca gtaactgtac aatatgacaa 3120
tgataaagca aacacaatgg attatacaaa ttggagtgaa atatatatta ttgaggaaac 3180
aacatgtact ttggtagcag gagaagttga ctatgtgggg ttgtattata tacatggcaa 3240
tgaaaagacg tattttaaat attttaaaga ggatgcaaaa aagtactcta aaacacaatt 3300
atgggaggta catgtgggta gtcgggtaat tgtatgtcct acatctatac ctagtgatca 3360
aatatccact actgaaactg ctgacccaaa gaccaccgag gccaccaaca acgaaagtac 3420
acaggggaca aagcgacgac gactcgattt accagactcc agagacaaca cccagtactc 3480
cacaaagtat acagactgcg ccgtggacag tagaccacga ggaggaggac tacacagtac 3540
aactaactgt acatacaaag ggcggaacgt gtgtagttct aaagtttcac ctatcgtgca 3600
tttaaaaggt gacccaaata gtttaaaatg tttaagatat agattaaaac catttaaaga 3660
cttatactgt aatatgtcat ccacatggca ttggaccagt gatgacaaag gtgacaaagt 3720
aggaattgtt actgtaacat acacaacgga aacacaacga caactgtttt taaacactgt 3780
taaaatacca cccactgtgc aaataagtac tggtgttatg tcattgtaat tgtattgtac 3840
aattactgta tgtaaaccac aagccaatat gtgctgctaa gtgtatatac aatgatatta 3900
cctatttttg ttgtttgttt tatactgttt ttatgcttgt gcattttttt gcggccattg 3960
gtgctatcta tttctatata tgcttggttg ctggtgttgg tgttgctgct ttgggtgtct 4020
gtggggtcgg ctctacgaat ttttttctgt tacttaatat ttttatatat accaatgatg 4080
tgtattaatt ttcatgcaca atacttaacc caacaagact aactgtatac tggttctgca 4140
catggtggta tggtattgta aatatttact gttgtgtgtg ttgtttttat tatttttata 4200
catttactaa taaatacttt tatattttta gcactgtctt attatgagac acaaacggtc 4260
tacaaggcgc aagcgtgcat ctgctacaca actttaccaa acatgcaagg cctcaggcac 4320
ctgcccacct gatgttatac ccaaagttga aggcactact atagcagatc aaatattacg 4380
atatggtagc ttaggggtgt tttttggagg tttaggcatt ggtacagggt cgggtacagg 4440
tggcaggact ggatatgtgc cccttggtag taccccaccg tctgaggcta tacctttaca 4500
gcccatacgt cccccagtta ccgttgatac tgtggggcct ttggattctt ctattgtatc 4560
tttaatagag gaatctagtt ttatagacgc cggtgcacca gccccatcaa ttcccactcc 4620
atctggtttt gatattacca cctctgcaga tactacacct gcaatactta atgtttcctc 4680
tattggagaa tcatctatac aaactgtttc tacacattta aatccctcct ttactgagcc 4740
atccgtactc cgccctcctg cacctgcaga ggcctctgga catttaatat tttcctctcc 4800
tactgttagc acacatagtt atgaaaacat accaatggat acctttgtta tttctactga 4860
cagtggcaat gtcacgtcta gcacacccat tccagggtct cgccctgtgg cacgccttgg 4920
tttatacagt cgcaacaccc aacaagttaa ggttgttgac cctgcttttt taacatctcc 4980
tcatagactt gtaacatatg ataatccagc atttgaaggc tttaaccctg aggacacatt 5040
gcagtttcaa catagtgaca tatcgcctgc tcctgatcct gattttctag atattgttgc 5100
attacacaga cctgcattaa cctctcgcag gggtactgta cgttatagta gggttgggca 5160
aaaggctaca cttcgtactc gcagtggaaa gcaaataggg gctaaagtac attactacca 5220
agacttaagt cccatacagc ctgtccagga acaggtacaa cagcagcaac aatttgaatt 5280
acaatcttta aatacttctg tttctcccta tagtattaat gatggacttt atgatattta 5340
tgctgacgat gctgatacta tacatgattt tcagagtcct ctgcactcac atacgtcctt 5400
tgccaccaca cgtaccagta atgtgtccat accattaaat actggatttg acactcctct 5460
tgtgtcattg gaacctggtc cagacattgc atcttctgta acatctatgt ctagtccatt 5520
tattcctata tctccactaa ctccttttaa taccataatt gtggatggtg ctgattttat 5580
gttgcaccct agctatttta ttttgcgtcg cagacgtaaa cgttttccat atttttttgc 5640
agatgtccgt gtggcggcct agtgaggcca ctgtgtacct gcctcctgtg cctgtgtcta 5700
aggttgtaag cactgatgaa tatgtgtcac gcacaagcat ttattattat gctggcagtt 5760
ccagactttt ggctgttggc aatccatatt tttccatcaa aagtcccaat aacaataaaa 5820
aagtattagt tcccaaggta tcaggcttac agtatagggt ctttagggtg cgtttacctg 5880
atcccaataa atttggtttt cctgatacat ctttttataa ccctgataca caacgtttgg 5940
tctgggcatg tgtaggcctt gaaataggta ggggacagcc attgggtgtt ggcgtaagtg 6000
gtcatcctta tttaaataaa tttgatgaca ctgaaaccag taacagatat cccgcacagc 6060
cagggtctga taacagggaa tgcttatcta tggattataa acaaacacaa ttatgtttaa 6120
ttggctgtaa acctcccact ggtgagcatt ggggtaaagg tgttgcctgt aacaataatg 6180
cagctgctac tgattgtcct ccattggaac tttttaattc tattattgag gatggtgaca 6240
tggtagatac agggtttgga tgcatggact ttggtacatt gcaggctaat aaaagtgatg 6300
tgcctattga tatttgtaac agtacatgca aatatccaga ttatttaaaa atggccagtg 6360
aaccttatgg ggatagtttg ttcttttttc ttagacgtga gcagatgttt gttagacact 6420
tttttaatag ggctggaaaa cttggcgagg ctgtcccgga tgacctttat attaaagggt 6480
ccggtaatac tgcagttatc caaagtagtg cattttttcc aactcctagt ggctctatag 6540
ttacctcaga atcacaatta tttaataagc cttattggct acagcgtgca caaggtcata 6600
acaatggcat ttgctggggc aatcagttat ttgttaccgt ggttgatacc actcgtagca 6660
ctaatatgac attatgcact gaagtaacta aggaaggtac atataaaaat gataatttta 6720
aggaatatgt acgtcatgtt gaagaatatg acttacagtt tgtttttcag ctttgcaaaa 6780
ttacactaac tgcagagata atgacatata tacatactat ggattccaat attttggagg 6840
actggcaatt tggtttaaca cctcctccgt ctgccagttt acaggacaca tatagatttg 6900
ttacctccca ggctattact tgccaaaaaa cagcaccccc taaagaaaag gaagatccat 6960
taaataaata tactttttgg gaggttaact taaaggaaaa gttttctgca gatctagatc 7020
agtttccttt gggacgaaag tttttattac aatcaggcct taaagcaaag cccagactaa 7080
aacgttcggc ccctactacc cgtgcaccat ccaccaaacg caaaaaggtt aaaaaataat 7140
tgttgtggta cttacactat tttattatac atgtttgttt gttttatgta tgtgttgtct 7200
gtttgtttat gtttgtgtat atgttgtatg tgttatgtgt catgtttgtg tacatgttct 7260
atgtccttgt cagtttcctg tttctgtata tatgtaataa actattgtgt gtattgtaaa 7320
ctatttgtat tgtttgggtg tatctatgag taaggtgctg tccctaaatt gccctaccct 7380
gccctgccta ttatgcatac ctatgtaata gtatttgtat gatatgtatt ttatagtttt 7440
taacagtact gcctccattt tactttacct ccattttgtg catgtaaccg atttcggttg 7500
ctggcacaaa cgtgtttttt ttaaactaca atttaaacaa tacagttaat cctttccctt 7560
cctgcactgc ttttgcctat acttgcatat gtgactcata tatacatgca gtgcagttgc 7620
aaaatgttta attatactca tagtttaaac atgcttatag gcacatattt taacttactt 7680
tcaatgctta agtgcagttt tggcttgcac aatagtttgt tatgccaaac tatgtcttgt 7740
aaaagtgact cactaacatt tattgccagg tgtggactaa ccgttttggg tcacattgtt 7800
catgtttcaa cattttatat aata 7824
<210> 15
<400> 15
000
<210> 16
<211> 24
<212> DNA
<213> Artificial
<220>
<223> Artificial Synthesis
<400> 16
attcggggga gaggcggttt gcgt 24
Claims (9)
1. The method for preparing the high-risk human papilloma virus capturing probe set is characterized by comprising the following steps of:
(1) synthesizing DNA sequences of 15 high-risk HPV and constructing a carrier, wherein the high-risk HPV is: one or more of HPV16, 18, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, and 68;
(2) synthesizing an amplification primer;
(3) performing rolling circle type amplification by using dNTP of a modified label as a raw material and the plasmids of the step (1) as templates and the primer of the step (2);
(4) quantifying and mixing the obtained amplification products;
(5) and (3) purifying the mixed product obtained in the step (4) and breaking the product by an ultrasonic breaking instrument.
2. The method according to claim 1, wherein the DNA sequences of the 15 high risk types of HPV are: one or more of SEQ ID NO. 1-15.
3. The method of claim 1 or 2, wherein the sequence of the primer in step (2) is: SEQ ID NO. 16.
4. The method of claim 1 or 2, wherein the dNTP source in step (3) comprises dATP, dGTP, dCTP, dTTP, and dUTP, wherein only dUTP is biotin-tagged.
5. The method of claim 1 or 2, wherein the enzyme used in the rolling circle amplification in step (3) is Phi29DNA polymerase.
6. The method of claim 1 or 2, wherein the amplification products are mixed in equal proportions in step (4).
7. The method of claim 1 or 2, wherein the parameters of the ultrasound in step (5) are set as: sonication was stopped for 30 seconds and 20 seconds for a total of 60 cycles.
8. An HPV capture probe prepared using the method of claim 1.
9. Use of the capture probe of claim 8 for detecting the HPV integration state, comprising the steps of:
(1) extracting sample DNA, fragmenting and constructing a DNA library;
(2) hybridizing the probe set prepared in the claim 1 with the constructed library to perform HPV sequence targeted capture and quantification;
(3) high-throughput sequencing;
(4) and bioinformatic analysis of gene sequences.
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| CN202210150665.5A CN114480739A (en) | 2022-02-18 | 2022-02-18 | Method for preparing human papilloma virus capture probe set and application thereof |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20140064035A (en) * | 2012-11-19 | 2014-05-28 | (주)다이오진 | A method for detecting oncogenic types of human papilloma virus using liquid beads array and multiplex pcr |
| CN104278022A (en) * | 2014-09-12 | 2015-01-14 | 中国人民解放军第四军医大学 | Preparation method and application of DNA capturing probe |
| CN110093454A (en) * | 2019-04-24 | 2019-08-06 | 南京格致医学检验有限公司 | The amplification sequencing approach analyzed for a variety of HPV Classification Identifications and genome conformity |
| CN111172159A (en) * | 2020-03-06 | 2020-05-19 | 佛山科学技术学院 | Bovine mitochondrial genome capture probe kit |
| CN111996235A (en) * | 2020-08-25 | 2020-11-27 | 广州鼓润医疗科技有限公司 | Detection probe, preparation method and application thereof |
-
2022
- 2022-02-18 CN CN202210150665.5A patent/CN114480739A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20140064035A (en) * | 2012-11-19 | 2014-05-28 | (주)다이오진 | A method for detecting oncogenic types of human papilloma virus using liquid beads array and multiplex pcr |
| CN104278022A (en) * | 2014-09-12 | 2015-01-14 | 中国人民解放军第四军医大学 | Preparation method and application of DNA capturing probe |
| CN110093454A (en) * | 2019-04-24 | 2019-08-06 | 南京格致医学检验有限公司 | The amplification sequencing approach analyzed for a variety of HPV Classification Identifications and genome conformity |
| CN111172159A (en) * | 2020-03-06 | 2020-05-19 | 佛山科学技术学院 | Bovine mitochondrial genome capture probe kit |
| CN111996235A (en) * | 2020-08-25 | 2020-11-27 | 广州鼓润医疗科技有限公司 | Detection probe, preparation method and application thereof |
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