CN108676797B - For detecting the reagent set and method of human papilloma virus - Google Patents

For detecting the reagent set and method of human papilloma virus Download PDF

Info

Publication number
CN108676797B
CN108676797B CN201810580442.6A CN201810580442A CN108676797B CN 108676797 B CN108676797 B CN 108676797B CN 201810580442 A CN201810580442 A CN 201810580442A CN 108676797 B CN108676797 B CN 108676797B
Authority
CN
China
Prior art keywords
dna
taggttcc
artificial sequence
solute
solvent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201810580442.6A
Other languages
Chinese (zh)
Other versions
CN108676797A (en
Inventor
伍建
姬晓雯
刘娜
王晓斌
郑生伟
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Chongqing maijinuo medical laboratory Co.,Ltd.
Original Assignee
Makino (chongqing) Gene Technology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Makino (chongqing) Gene Technology Co Ltd filed Critical Makino (chongqing) Gene Technology Co Ltd
Priority to CN201810580442.6A priority Critical patent/CN108676797B/en
Publication of CN108676797A publication Critical patent/CN108676797A/en
Application granted granted Critical
Publication of CN108676797B publication Critical patent/CN108676797B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/708—Specific hybridization probes for papilloma
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer

Abstract

The invention discloses the reagent sets and method for detecting human papilloma virus.Reagent set disclosed by the invention for detecting human papilloma virus is made of 621 probes, the sequence of this 621 probes is sequence 1-621 in sequence table, 1-15 and 94-108 of every sequence of sequence 1-621 are all the same, can be used for expanding 621 probes；5 ' ends of every probe can be marked with biotin.Reagent set of the invention is in capture Human Papillomavirus DNA group and sequencing, its different parting can be distinguished, it can also determine whether its DNA is integrated into human genome, it can also determine the copy number of human papilloma virus, and has the characteristics that parting is accurate, can get and integrate information and copy number, high sensitivity, meet the development trend of accurate medical treatment, meaning is very great.

Description

For detecting the reagent set and method of human papilloma virus
Technical field
The present invention relates in cma gene engineering and molecular genetics field, for detecting the complete examination of human papilloma virus Agent and method.
Background technique
Human papilloma virus (Human Papillomavirus, HPV) belongs to Papillomaviridae, is a kind of small molecule , without envelope coated circular double stranded DNA virus, genome is about 8000 base-pairs (bp), is divided into 3 functional areas, i.e. early stage Transcriptional domain (area E), late transcription area (area L) and nontranscribed domain (long control area, LCR).HPV passes through direct or indirect contact stain Article or the Sex transmitted pathogen mankind.The virus not only has host specificity, and has tissue specificity, can only infect people's Skin and mucomembranous epithelial cell, cause human skin a variety of papillomas or wart and genital tract epithelial proliferation damage.
It, can according to the other pathogenicity size of each genotype or carcinogenic risk size for infecting the HPV of genital tract and anus It is divided into low risk and high-risk-type two major classes.About the division of high-risk-type and low risk, according to WHO international cancer research institution (IARC) and the research achievement of other international organizations, HPV16,18,31,33,35,39,45,51,52,56,58,59,68 etc. 13 Kind genotype is classified as high-risk gene type, and 5 kinds of genotype such as HPV26,53,66,73,82 are classified as middle danger gene type, HPV6,11 Genotype is classified as low danger gene type.
Genital HPV infection has generality in the women of sexual intercourse history, and 70%~80% women exists according to statistics It has HPV infection at least once in life, but most of infection are self limiting, and the women of the infection more than 90% will appear A kind of effective immune response can remove infection in no any long-term Health intervention between 6 to 24 months.And it holds The high-risk HPV infection of continuous property is then the main reason for leading to Cervical intraepitheliaI neoplasia and cervical carcinoma.The research knot of global range Fruit shows, 99.7% cervical cancer patient vivo detection to the presence of high-risk HPV DNA, wherein HPV16 type, 18 types, 45 Type and the infection of 31 types account for 80%.
For many years, in the world the diagnosis of general Cervical intraepitheliaI neoplasia and cervical carcinoma essentially according to cervical cytology, yin Road mirror and histopathological examination.Cervical cytological examination is the screening side of commonly used Cervical intraepitheliaI neoplasia and cervical carcinoma Method, it is possible to find early lesion.But there are its intrinsic limitations for cervical cytological examination.Although low risk HPV seldom causes to soak Moisten cancer, but generally related to condyloma acuminatum or low squamous intraepithelial lesion.
In consideration of it, the genotyping detection method for seeking HPV is used for the shunting and cervical carcinoma of cervical cytological examination patients with abnormal Screening can effectively increase cervical lesions recall rate, improve cytolgical examination sensibility, and reduce screening frequency.And to palace Neck cancer treatment and judging prognosis are meaningful, while causing the mechanism study of cancer also to have very great help perfect virus.
HPV viruse is also related to progression of disease in the intracorporal existence form of people.HPV viruse is after entering human body, DNA meeting Be broken into it is single-stranded, can be integrated into the genome of people in the form being embedded in.This integration not only results in the DNA sequence in insertion site Column mutate, and cell can replicate in division and breeding together with HPV viruse genome, so as to cause HPV disease Poison is escaped immune system and is removed.Studies have found that the integration of HPV and host chromosome is one in cervical cell immortalization process A important step, and lead to an important symbol of the Cervical intraepitheliaI neoplasia to cervical carcinoma malignant progression.It is good caused by HPV During venereal disease becomes, intracellular viral DNA will not be generally integrated in host genome.But in the change of high-level endothelioma and uterine neck In cancer, HPV DNA is often integrated in host chromosome, and as cervical lesions develop, integration is dramatically increased.Therefore, HPV viruse Integrated state detection in host cell is also the important parameter of assessment cervical lesions and prognosis.
At present on the market, HPV nucleic acid detection technique mainly includes biochip method, digestion signal amplification method, PCR- fluorescence Sonde method, the nucleic acid amplification technologies of transcriptive intermediate and PCR- hybrid method etc..
However it these HPV nucleic acid detection means or only detects HPV nucleic acid information, can not detect HPV integrated state, or exist Susceptibility is low, it is cumbersome, time-consuming, need great amount of samples, detection HPV genotype few and determine the disadvantages of result is uncertain, such as Southern hybridization, real-time quantitative PCR, genetic chip, amplification HPV integrate the detection methods such as oncogene transcripton.In two generations, surveyed The development of sequence (next generation sequencing, NGS) technology, so that high-throughput, large-scale HPV integrated state Detection is possibly realized.
Summary of the invention
The technical problem to be solved by the present invention is to how detect human papilloma virus.
In order to solve the above technical problems, the reagent set is denoted as complete present invention firstly provides a kind of reagent set Reagent 1, the reagent set 1 are made of 621 probes, and the sequence of 621 probes is respectively sequence 1-621 in sequence table 16-93.
The present invention also provides another reagent sets, which is denoted as reagent set 2, the reagent set 2 It is made of 621 probes, 16-93 respectively containing sequence 1-621 in ordered list of 621 articles of probes.
Identification sequence of 621 probes of the reagent set 2 also containing the primer for amplification probe.It is described The sequence of 621 probes concretely sequence 1-621 in sequence table.Wherein, the 1-15 of every sequence of sequence 1-621 Position and 94-108 it is all the same, can be used for expanding 621 probes.
5 ' ends of every probe in above-mentioned reagent set 1 and reagent set 2 can be marked with biotin.
Above-mentioned reagent set 1 and reagent set 2 are used equally for detection human papilloma virus.
In above-mentioned reagent set 1 and reagent set 2, all probes can be packaged together, the molal quantity of each probe It is equal.
The present invention also provides the system for detecting human papilloma virus, the system comprises the reagent set 1 or The reagent set 2.
The system may also include M1, and the M1 is harvesting buffer, hybridization buffer and/or combination buffer；
The harvesting buffer is made of solvent and solute, and the solvent is water, the solute behaviour cot-1DNA, salmon Smart DNA, primer 1 and primer 2, the primer 1 are single stranded DNA shown in sequence 622 in sequence table, and the primer 2 is sequence table Single stranded DNA shown in middle sequence 623；
The hybridization buffer is made of solvent and solute, and the solvent is 50mM-200mM Tris-HCl (pH 7.6), The solute and its concentration in the hybridization buffer are respectively 1.25M NaCl, 0.125M sodium citrate, 0.1g/ 100mL BSA, 5%-10% (volume ratio) Tween20；Wherein, the 50mM-200mM Tris-HCl (pH 7.6) is by solvent With solute form, the solvent be water, the solute be trishydroxymethylaminomethane (Tris) and the HCl for adjusting pH, three The concentration of hydroxymethyl aminomethane is 50mM-200mM, pH 7.6；
The combination buffer is made of solvent and solute, and the solvent is 10mM Tris-HCl (pH 7.5), described molten Matter and its concentration are respectively 1M NaCl, 1mM EDTA；The 10mM Tris-HCl (pH 7.5) is made of solvent and solute, institute Stating solvent is water, and the solute is trishydroxymethylaminomethane (Tris) and the HCl for adjusting pH, trishydroxymethylaminomethane Concentration be 10mM, pH 7.5.
In above system, the content of solute described in the harvesting buffer can be the people cot- of Invitrogen respectively 1DNA 35% (volume ratio), the salmon sperm dna 15% (volume ratio) of Invitrogen, 1 0.5nmol/ μ l of the primer and institute State primer 2 0.5nmol/ μ l；
In the hybridization buffer, the solvent is 100mM Tris-HCl (pH 7.6), the solute and its described Concentration in hybridization buffer is respectively 1.25M NaCl, 0.125M sodium citrate, 0.1g/100mL BSA, 7% (volume ratio) Tween20；The 100mM Tris-HCl (pH 7.6) is made of solvent and solute, and the solvent is water, and the solute is three Hydroxymethyl aminomethane and HCl for adjusting pH, the concentration of trishydroxymethylaminomethane are 100mM, pH 7.6.
The system can further include M2, and the M2 is rinsing liquid 1, rinsing liquid 2, NaOH solution (0.1M), Tris-HCl are slow At least one of fliud flushing (1M, pH 7.5), PCR reaction solution and TE buffer；
The rinsing liquid 1 is made of solvent and solute, and the solvent is 1 × SSC solution, and the solute is SDS, and SDS exists Mass percent concentration in the rinsing liquid 1 is 0.1%；1 × SSC solution is made of solvent and solute, the solvent It is respectively NaCl 175g/L and trisodium citrate 88g/L for water, the solute and its concentration in 1 × SSC solution, pH 7.4；
The rinsing liquid 2 is made of solvent and solute, and the solvent is 0.1 × SSC solution, and the solute is SDS, SDS Mass percent concentration in the rinsing liquid 2 is 0.1%；Wherein, 0.1 × SSC solution is that the 1 × SSC is molten Liquid dilutes 10 times of obtained solution；
The NaOH solution (0.1M) is made of solvent and solute, and the solvent is water, the solute and in the NaOH Concentration in solution (0.1M) is 0.1M NaOH；
The Tris-HCl buffer (1M, pH 7.5) is made of solvent and solute, and the solvent is water, and the solute is Trishydroxymethylaminomethane and HCl for adjusting pH, the concentration of trishydroxymethylaminomethane are 1M, pH 7.5；
The PCR reaction solution is made of solvent and solute, and the solvent is the Phusion buffer of 1 times of concentration, described molten Matter and its concentration in the PCR reaction solution are respectively 0.2mM dATP, 0.2mM dTTP, 0.2mM dCTP, 0.2mM DGTP, 2.5pmol primer 3,2.5pmol primer 4,0.05U/ μ l Hotstart Phusion enzyme, 5% (volume ratio) DMSO；Its In, the Phusion buffer of 1 times of concentration is made of solvent and solute, and the solvent is 50mM Tris-HCl (pH 8.8), the solute and its concentration in the Phusion buffer of 1 times of concentration are respectively 4mM MgCl2, 500mM KCl, 0.8% (v/v) Nonidet P40；The 50mM Tris-HCl (pH 8.8) is made of solvent and solute, the solvent For water, the solute is trishydroxymethylaminomethane and the HCl for adjusting pH, and the concentration of trishydroxymethylaminomethane is 50mM, pH 8.8；The sequence of the primer 3 and the primer 4 can be respectively sequence 624 and 625 in sequence table, the primer 3 Thio-modification can be had with last nucleotide of the primer 4；
The TE buffer is made of solvent and solute, the solvent be water, the solute be trishydroxymethylaminomethane, EDTA and HCl for adjusting pH, the concentration of trishydroxymethylaminomethane are 10mM, and the concentration of EDTA is 1mM, pH 8.0.
The Phusion buffer of 1 times of concentration is concretely by 5 times of concentration of New England Biolabs Phusion buffer dilutes 5 times of obtained solution.
The Hotstart Phusion enzyme concretely New England Biolabs product.
Above system can be only made of the reagent set 1 or the reagent set 2 and M1, can also be by described complete Reagent 1 or the reagent set 2 and the M1 and the M2 form.
The system can be kit.
The present invention also provides following either method:
X1) it is enriched with the method for Human Papillomavirus DNA group DNA, comprising: utilize the reagent set 1 or the complete examination Agent 2 captures sample to be tested Human Papillomavirus DNA group DNA, realizes the enrichment of Human Papillomavirus DNA group DNA；
X2 the method for Human Papillomavirus DNA group sequence) is detected, comprising: utilize the reagent set 1 or described complete Reagent 2 captures sample to be tested Human Papillomavirus DNA group DNA, the genomic DNA captured, to the base captured Because a group DNA is sequenced, Human Papillomavirus DNA group sequence is obtained；
X3 the method for typing of human papillomavirus) is detected, comprising: catch using the reagent set 1 or the reagent set 2 Obtain sample to be tested Human Papillomavirus DNA group DNA, the genomic DNA captured, to the genomic DNA captured It is sequenced, obtains sample to be tested Human Papillomavirus DNA group sequence；By the sample to be tested Human Papillomavirus DNA group Sequence is compared with Human Papillomavirus DNA group reference sequences, obtains the typing of human papillomavirus of the sample to be tested；
X4 the method for human papillomavirus HPV DNA insertion point in human genome) is detected, comprising: utilize the complete examination Agent 1 or the reagent set 2 capture sample gene to be tested group DNA, the genomic DNA captured, capture to described Genomic DNA is sequenced, and sequencing sequence is obtained；By the sequencing sequence and Human Papillomavirus DNA group reference sequences and Ginseng is examined genome sequence and is compared, and determines insertion point of the HPV DNA of the sample to be tested in human genome；
X5 the method for HPV copy number) is detected, comprising: capture using the reagent set 1 or the reagent set 2 to be measured Sample genomic dna, the genomic DNA captured；The genomic DNA captured is sequenced, determines HPV's Copy number.
X5 in), the genomic DNA captured is sequenced, determines that the copy number of HPV is specific can include: to institute It states the genomic DNA captured to be sequenced, obtains HPV average sequencing depth and HPV capture enrichment multiplying power；By to it is described to The genomic DNA of test sample sheet is sequenced, and then compares sequencing result and human genome, obtains human genome and depth is averagely sequenced Degree；(1) calculates HPV copy number according to the following formula；
HPV copy number=HPV average sequencing depth/(human genome average sequencing depth × HPV capture enrichment multiplying power) is (public Formula (1)).
Wherein, HPV capture enrichment multiplying power refers in the genomic DNA captured the content of HPV DNA and described to test sample The ratio of the content of HPV DNA in this genomic DNA.In one embodiment of the invention, HPV capture enrichment multiplying power is 46000。
The typing of human papillomavirus can for HPV 6,11,16,18,31,33,35,39,45,52,56,58,59,66, 68, at least one of 69,82,51,26,30,34,53,67,70,73,85 and 97.
The present invention also provides following any applications of the reagent set 1 or the reagent set 2:
Y1) the application in preparation detection Human Papillomavirus DNA group serial products；
Y2) the application in detection Human Papillomavirus DNA group sequence；
Y3 it) is applied in preparation detection typing of human papillomavirus product；
Y4 it) is applied in detection typing of human papillomavirus；
Y5) the application in preparation detection human papillomavirus HPV DNA in human genome in insertion point product；
Y6) the application in detection human papillomavirus HPV DNA in human genome in insertion point；
Y7) the application in preparation detection HPV copy number product；
Y8) the application in detection HPV copy number.
The product can be kit.
The present invention also provides following any applications of the system:
Z1) the application in detection Human Papillomavirus DNA group sequence；
Z2 it) is applied in detection typing of human papillomavirus；
Z3) the application in detection human papillomavirus HPV DNA in human genome in insertion point；
Z4) the application in detection HPV copy number.
Reagent set for detecting human papilloma virus of the invention is in capture Human Papillomavirus DNA group and sequencing In, its different parting can be distinguished, can also determine whether human papillomavirus HPV DNA is integrated into human genome, if there is integration Situation can detecte specific integration site and integration sequence, can also determine the copy number of human papilloma virus, and have Parting is accurate, can get the features such as integrating information and copy number, high sensitivity.It can be examined simultaneously using reagent set of the invention 27 HPV gene types are surveyed, further include middle danger genotype including the high-risk gene type HPV closely related with cervical carcinoma Low danger gene type other and relevant to condyloma acuminatum or low squamous intraepithelial lesion, can be used for detecting related to HPV infection Various diseases, such as cervical carcinoma, carcinoma of vagina, carcinoma of vulva, anus early carcinoma.Reagent set of the invention can be applied to uterine neck The idicatio, Atypical Squamous epithelium of prediction, the vaginoscopy of Residual or recurrent lesion after cancer and precancerous lesion screening, treatment Cell patient shunts and carries out corresponding health consultation, probes into HPV carcinogenic mechanism etc., meets the development trend of accurate medical treatment, Meaning is very great.
Detailed description of the invention
Fig. 1 is integration site figure of the sample 17O010099 at chr11:129325933.(01) row is HPV with reference to base Because of group position and base sequence；(02)-(06) row is sequencing reading length sequence names and base sequence；(07) row is that ginseng is examined Genomic locations and base sequence；(08)-(12) row is sequencing reading length sequence names and base sequence.
Fig. 2 is integration site figure of the sample 17O010099 at chr11:129339149.(01) row is that ginseng examines base Because of group position and base sequence；(02)-(06) row is sequencing reading length sequence names and base sequence；(07) row is HPV ginseng Examine genomic locations and base sequence；(08)-(12) row is sequencing reading length sequence names and base sequence.
Fig. 3 is integration site figure of the sample 17O010099 at chr11:129319998.(01) row is HPV with reference to base Because of group position and base sequence；(02)-(05) row is sequencing reading length sequence names and base sequence；(06) row is that ginseng is examined Genomic locations and base sequence；(07)-(10) row is sequencing reading length sequence names and base sequence.
Fig. 4 is integration site figure of the sample 17O011102 at chr2:191437654.(01) row is HPV with reference to base Because of group position and base sequence；(02)-(06) row is sequencing reading length sequence names and base sequence；(07) row is that ginseng is examined Genomic locations and base sequence；(08)-(12) row is sequencing reading length sequence names and base sequence.
Fig. 5 is integration site figure of the sample 17O011102 at chr2:191452577.(01) row is that ginseng examines gene Group position and base sequence；(02)-(06) row is sequencing reading length sequence names and base sequence；(07) row is HPV reference Genomic locations and base sequence；(08)-(12) row is sequencing reading length sequence names and base sequence.
Fig. 6 is integration site figure of the sample 17O011102 at chr2:191462814.(01) row is that ginseng examines gene Group position and base sequence；(02)-(06) row is sequencing reading length sequence names and base sequence；(07) row is HPV reference Genomic locations and base sequence；(08)-(12) row is sequencing reading length sequence names and base sequence.
Fig. 7 is integration site figure of the sample 17O011228 at chr4:172351833.(01) row is HPV with reference to base Because of group position and base sequence；(02)-(06) row is sequencing reading length sequence names and base sequence；(07) row is that ginseng is examined Genomic locations and base sequence；(08)-(12) row is sequencing reading length sequence names and base sequence.
Fig. 8 is integration site figure of the sample 17O011808 at chr1:142934469.(01) row is that ginseng examines gene Group position and base sequence；(02)-(06) row is sequencing reading length sequence names and base sequence；(07) row is HPV reference Genomic locations and base sequence；(08)-(12) row is sequencing reading length sequence names and base sequence.
Fig. 9 is integration site figure of the sample 17O011808 at chr4:151085095.(01) row is HPV with reference to base Because of group position and base sequence；(02)-(06) row is sequencing reading length sequence names and base sequence；(07) row is that ginseng is examined Genomic locations and base sequence；(08)-(12) row is sequencing reading length sequence names and base sequence.
Figure 10 is integration site figure of the sample 17O010971 at chr2:109387815.(01) row is HPV with reference to base Because of group position and base sequence；(02)-(07) row is sequencing reading length sequence names and base sequence；(08) row is that ginseng is examined Genomic locations and base sequence；(09)-(14) row is sequencing reading length sequence names and base sequence.
Specific embodiment
The present invention is further described in detail With reference to embodiment, and the embodiment provided is only for explaining The bright present invention, the range being not intended to be limiting of the invention.Experimental method in following embodiments is unless otherwise specified Conventional method.Material as used in the following examples, reagent, instrument etc., are commercially available unless otherwise specified. Quantitative test in following embodiment, is respectively provided with three repeated experiments, and results are averaged.In following embodiments, such as without special Illustrate, the 1st of each nucleotide sequence is the 5 ' terminal nucleotides of corresponding DNA in sequence table, and last bit is the 3 ' of corresponding DNA Terminal nucleotide.
The preparation of the reagent set and kit of embodiment 1, detection human papilloma virus
1, the reagent set of human papilloma virus is detected
The reagent set of detection human papilloma virus provided in this embodiment, designs according to 27 kinds of HPV gene types and is used in combination In identifying this 27 kinds of HPV gene types, this 27 kinds of HPV gene types be respectively HPV 6,11,16,18,31,33,35,39,45, 52,56,58,59,66,68,69,82,51,26,30,34,53,67,70,73,85 and 97.Detect the complete of human papilloma virus Reagent is made of 621 probes, the sequences of this 621 probes is sequence 1-621 in sequence table, and 16-93 of every sequence 1-15 and 94-108 of human papilloma virus for identification, every sequence are all the same, can be used to amplification probe, often 5 ' ends of probe are by biotin labeling.
In the reagent set, all probes are packaged together, and the molal quantity of each probe is equal.
2, the kit of human papilloma virus is detected
It is provided in this embodiment detection human papilloma virus kit by detection human papilloma virus reagent set with Following reagent composition: harvesting buffer, hybridization buffer, combination buffer, rinsing liquid 1, rinsing liquid 2, NaOH solution (0.1M), Tris-HCl buffer (1M, pH 7.5), PCR reaction solution, TE buffer.Wherein each solution composition is as follows:
(1) harvesting buffer:
Harvesting buffer is made of solvent and solute, and solvent is water, and solute and its concentration are people cot-1DNA respectively (Invitrogen) 35% (volume ratio), salmon sperm dna (Invitrogen) 15% (volume ratio), 1 0.5nmol/ μ l of primer and Primer 2 0.5nmol/ μ l, wherein the sequence of primer 1 and primer 2 is as follows:
Primer 1:AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCT (sequence in sequence table 622)；
Primer 2: CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGC (sequence in sequence table 623)；
(2) hybridization buffer:
Hybridization buffer is made of solvent and solute, and solvent is 100mM Tris-HCl (pH 7.6), solute and its concentration Respectively 1.25M NaCl, 0.125M sodium citrate, 0.1g/100mL BSA, 7% (volume ratio) Tween20；Wherein, 100mMTris-HCl (pH 7.6) is made of solvent and solute, and solvent is water, solute be trishydroxymethylaminomethane (Tris) and For adjusting the HCl of pH, the concentration of Tris is 100mM, pH 7.6；
(3) combination buffer:
Combination buffer is made of solvent and solute, and solvent is 10mM Tris-HCl (pH 7.5), solute and its concentration point It Wei not 1M NaCl, 1mM EDTA；10mM Tris-HCl (pH 7.5) is made of solvent and solute, and solvent is water, solute three The concentration of hydroxymethyl aminomethane (Tris) and the HCl for adjusting pH, Tris are 10mM, pH 7.5；
(4) rinsing liquid 1:
Rinsing liquid 1 is made of solvent and solute, and solvent is 1 × SSC solution, the matter of solute SDS, SDS in rinsing liquid 1 Measuring percent concentration is 0.1%；Wherein, 1 × SSC solution is made of solvent and solute, and solvent is water, solute and its concentration difference For NaCl 175g/L and trisodium citrate 88g/L, pH 7.4；
(5) rinsing liquid 2:
Rinsing liquid 2 is made of solvent and solute, and solvent is 0.1 × SSC solution, and solute SDS, SDS are in rinsing liquid 2 Mass percent concentration is 0.1%；Wherein, 0.1 × SSC solution is that 1 × SSC solution is diluted 10 times of obtained solution；
(6) NaOH solution (0.1M)
NaOH solution (0.1M) is made of solvent and solute, and solvent is water, and solute and its concentration are 0.1M NaOH；
(7) Tris-HCl buffer (1M, pH 7.5)
Tris-HCl buffer (1M, pH 7.5) is made of solvent and solute, and solvent is water, and solute is trihydroxy methyl amino The concentration of methane (Tris) and the HCl for adjusting pH, Tris are 1M, pH 7.5；
(8) PCR reaction solution:
PCR reaction solution is made of solvent and solute, and solvent is Phusion buffer (the New England of 1 times of concentration Biolabs), solute and its concentration be respectively 0.2mM dATP, 0.2mM dTTP, 0.2mM dCTP, 0.2mM dGTP, 2.5pmol primer 3,2.5pmol primer 4,0.05U/ μ l Hotstart Phusion enzyme (New England Biolabs), 5% (volume ratio) DMSO；Wherein, the Phusion buffer of 1 times of concentration is made of solvent and solute, and solvent is 50mM Tris- HCl (pH 8.8), solute and its concentration are respectively 4mM MgCl2, 500mM KCl, 0.8% (v/v) Nonidet P40；50mM Tris-HCl (pH 8.8) is made of solvent and solute, and solvent is water, and solute is trishydroxymethylaminomethane (Tris) and is used for The HCl of pH is adjusted, the concentration of Tris is 50mM, pH 8.8.
Wherein, primer sequence is as follows:
Primer 3:AATGATACGGCGACCACCGA*G (sequence 624 in sequence table)；
Primer 4:CAAGCAGAAGACGGCATACG*A (sequence 625 in sequence table)；* thio-modification is indicated.
(9) TE buffer
TE buffer is made of solvent and solute, and solvent is water, solute Tris, EDTA and the HCl for adjusting pH, The concentration of Tris is 10mM, and the concentration of EDTA is 1mM, pH 8.0.
Embodiment 2, the method for detecting human papilloma virus
The method of reagent set and kit detection human papilloma virus using embodiment 1 of the invention is as follows:
1, the preparation in full-length genome library
Through patient's informed consent, the genomic DNA of the peripheral blood of HPV viruse infected individuals, the genome that will be obtained are extracted DNA concentration is adjusted to 75ng/ μ l, and total amount 3-5 μ g DNA is interrupted at random, utilizes KAPA Hyper library construction Kit (article No. Full-length genome library is constructed for KK8504).The sequence of two single stranded DNAs of connector used is as follows:
Single stranded DNA 1:AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCG ATCT
Single stranded DNA 2:AGATCGGAAGAGCACACGTCTGAACTCCAGTCACNNNNNNNNATCTCGTATGCC GTCTT CTGCTTG, wherein N A, T, C or G.
2, the specificity capture and sequencing of HPV genetic fragment
1) 13 μ l harvesting buffers and 5 μ l reagent set solution are added in the full-length genome library for taking 1 μ g step 1 thereto (solution is the liquid that the reagent set for dissolving embodiment 1 using water obtains, and the total concentration of the solution middle probe is 100ng/ μ L), obtained reaction system is placed in PCR instrument, reaction condition is 95 DEG C of 7min, later 65 DEG C of 2min, and reaction terminates to obtain anti- Answer product；
2) the 23 μ l of hybridization buffer that 65 DEG C preheat is added to above-mentioned steps 1) in obtained reaction product, then in Hybridize 22 hours for 65 DEG C in PCR instrument, obtains enrichment system mixture；
3) so that magnetic bead is sufficiently suspended the concussion of MyOne C1 Streptavidin MagneSphere (Invitrogen) whirlpool, take 50 μ l Centrifuge tube of the MyOne C1 Streptavidin MagneSphere to new 1.5ml；
4) at least by the 1.5ml centrifuge tube whirlpool concussion equipped with 50 μ l MyOne C1 Streptavidin MagneSpheres of step 3) 5s makes magnetic bead sufficiently suspend, and is put on magnetic frame and remain stationary one minute (should not rotating centrifugal pipe) after of short duration centrifugation, careful to inhale Abandon supernatant；
5) centrifuge tube of step 4) is removed, 50 μ l combination buffers are added thereto, rotation nest shakes at least 5s, of short duration centrifugation After be put on magnetic frame static one minute, careful inhale abandons supernatant, in triplicate；
6) centrifuge tube of step 5) is removed, the combination buffer of 2 times of concentration of 100 μ l is added thereto (i.e. by embodiment 1 The solution that each solute concentration of middle combination buffer doubles, i.e., each solute concentration is real in the combination buffer of 2 times concentration 2 times for applying the corresponding solute concentration of a combination buffer), rotation nest shakes at least 5s, is put into static one on magnetic frame after of short duration centrifugation Minute；
7) the enrichment system mixture that step 2) obtains is added in rapid centrifuge tube 6), rotation nest shakes at least 5s, so It is placed on room temperature on gyroscope and rotates 1 hour (60 revs/min)；
8) after the completion of step 7), utilize 1 room temperature cleaning step 7 of rinsing liquid) magnetic bead it is primary, 15 minutes, then again with drift Washing lotion 2 is cleaned 3 times, every time 15 minutes at 65 DEG C；
9) after the completion of step 8), magnetic bead is eluted 10 minutes at room temperature with NaOH solution (0.1M), is then washed what is obtained De- liquid whirlpool shakes at least 5s, is put into after of short duration centrifugation on magnetic frame static one minute, then supernatant is transferred to containing 70 μ In the clean centrifuge tube of l Tris-HCl buffer (1M, pH 7.5) to get arrive DNA solution；
10) DNA solution that step 9) obtains is carried out using Qiagen MinElute Column (Qiagen product) pure Change, DNA will be obtained using TE buffer solution, obtain purified DNA solution；
11) 70ul PCR reaction solution is added in the purified DNA solution obtained to 30 μ l steps 10) and carries out PCR amplification, obtain PCR product；
PCR reaction condition are as follows: 98 DEG C of 30s, 1 circulation；98 DEG C of 25s, 65 DEG C of 30s, 72 DEG C of 30s, 15 circulations；72℃ 5min, 1 circulation；
12) Agencourt AMPure XP Nucleic acid purification kits (Beckman Coulter) purification step 11 is utilized) PCR product, obtain purified product.
13) purified product that step 12) obtains is sequenced on 500 sequenator of Illumina NextSeq, it is used Sequencing primer is sequencing primer 1 and sequencing primer 2, sequencing primer 1:AATGATACGGCGACCACCGA；Sequencing primer 2:CAAGC AGAAG ACGGC ATACG AGAT。
3, bioinformatic analysis process and result output
According to the sequencing result of step 2 to the integration site of HPV viruse parting and determining HPV viruse:
(1) HPV viruse phenotypic analysis process
1. obtaining sequencing original series；
2. data prediction: anti-pollution connector and low quality data in removal sequencing data etc., used parameter :-m 40-q10,10-O 6-e 0.08, the reference of design parameter meaning:http://cutadapt.readthedocs.io/en/ stable/；
3. data after pretreatment are compared with all HPV with reference to genome with BWA tool, used parameter: bwa Mem-M, the reference of design parameter meaning:http://bio-bwa.sourceforge.net/bwa.shtml
4. counting sequencing result information, point of HPV viruse domain covering size, average sequencing depth and HPV viruse is determined Type etc.；
(2) HPV viruse confluence analysis process
1. choosing every kind of type according to the parting information of step (1) and comparing optimal plant type as HPV-Integration analysis With reference to genome；
2. data are compared with hg19 and HPV-Integration analysis with reference to genome progress bwa mem after screening, obtain Bam is compared as a result, used parameter: bwa mem-M, the reference of design parameter meaning:http://bio- bwa.sourceforge.net/bwa.shtml；
3. obtaining preliminary integration site according to bam is compared as a result, analyzing structral variations with SVdetect Region, design parameter meaning reference: http://svdetect.sourceforge.net/Site/Manual.html；
4. analyzing HPV integration site with CREST, CREST is analyzed into result and SVdetect analysis result integration, is obtained HPV viruse integrated results.
Embodiment 3 detects HPV genotyping result using the kit of embodiment 1
The HPV gene type of 12 cervical cancer patients (through patient's informed consent) is detected using the kit of embodiment 1, It is carried out according to the method for embodiment 2.
As a result, it was confirmed that the reagent set of embodiment 1 has high capture rate to HPV gene, target area (HPV gene) is put down Effectively sequencing READ amount reaches 20Mb, and the average sequencing depth of target area (HPV gene) is 1000X or more (being shown in Table 1), inspection The HPV gene type for measuring different patients infection is as shown in table 1, during the HPV gene type of detected patient's infection is The common HPV gene type in state continent.
Table 1, the HPV genotyping result that 12 cervical cancer patients are detected using kit of the present invention
With triumphant general 23 kinds of HPV fluorescence parting detecting reagent (fluorescent PCR method), (registration certificate number: state's tool note is quasi- 20153401700) sample above is detected, the HPV gene type of each sample is detected and is tied using the kit detection of embodiment 1 Fruit is completely the same.
Embodiment 4 detects HPV integration site using the kit of embodiment 1
The HPV parting of 5 cervical cancer patients (through patient's informed consent) is detected using the kit using embodiment 1, It is carried out according to the method for embodiment 2.
As a result it confirms to detect HPV gene type, and detects different HPV integration sites, including human genome is whole Coincidence point, HPV integration site integrate segment (being shown in Table 2 and Fig. 1-10).
Table 2, the HPV integration that part cervical cancer patient is detected using kit of the present invention
Respectively in the upstream and downstream design primer of each insertion point, and PCR amplification and Sanger survey are carried out to insertion point Sequence, primer sequence are as shown in table 3.The results show that the result of the result and Sanger sequencing obtained using the kit of embodiment 1 It is completely the same.
Table 3, primer sequence
Insertion point number Sample number into spectrum Forward primer (5 ' -3 ') Reverse primer (5 ' -3 ')
1 17O010099 GGTGCAGTTACCTGACCCAA GCAGAAGGGCAATGTGCTTG
2 17O010099 ACTGCGTCAACTAATGGGCA TCCTGCTTATTGCCACCACC
3 17O010099 GCCCTGTTGCGTTACAAATGT TGTAGTCTTGTAGCGTTCGAAGA
4 17O011102 TGGTACAGATTCTAGGTGGCC TGGTAACTGTCTTCTCAGAAACCA
5 17O011102 AATAGTGCGCTGCTTGTTGC TGCAGCAATACACCAATCGC
6 17O011102 TGTCTTCTTCGTTGTGGGGC TTGGCCAAGTGCTGCCTAAT
7 17O011228 AGAGGACGAGGATGAAAATGCA ACAAGAAAAGGATGGCATGACT
8 17O011808 AGCCTGCATTATGAAGGGCA TGACCTTGTGTGCTACGAGC
9 17O011808 CTGTGTTAGGCTGGTGCAGT CGCTGCATACGGTGTACAGT
10 17O010971 GAGGACGAAACAGCAGACGA GCAAATGCATGGATATTTTGGTAAACT
Embodiment 5 detects HPV copy number using the kit of embodiment 1
With normal human genome dna gradient dilution caski cell line dna sample, be utilized respectively the kit of embodiment 1 by HPV genome copy numbers are detected according to the method for embodiment 2.Then according to formula, [HPV copy number calculated value=HPV is averagely sequenced Depth (de-redundancy)/(human genome average sequencing depth × HPV capture enrichment multiplying power)] copy number is calculated, it as a result confirms to calculate The copy number of the HPV genome obtained and theoretical copy number (being shown in Table 4), show that kit of the invention can be also used for determining HPV copy number.Wherein, HPV capture enrichment multiplying power refers to the genomic DNA captured by the capture multiplying power gained repeatedly measured The ratio of the content of middle HPV DNA and the content of HPV DNA in the sample gene to be tested group DNA, is herein 46000；People's base Because group average sequencing depth is that sample is sequenced according to the full-length genome library that the method for 2 step 1 of embodiment obtains, gained The ratio of sequencing data size and human genome size is human genome average sequencing depth.
Table 4, the HPV copy number results that CaSki cell line gDNA gradient dilution is detected using the kit of embodiment 1
<110>Mai Jinuo (Chongqing) Gene science Co., Ltd
<120>for detecting the reagent set and method of human papilloma virus
<160> 625
<170> PatentIn version 3.5
<210> 1
<211> 108
<212> DNA
<213>artificial sequence
<400> 1
gactacatgg gacatatgga aagtgcaaat gcctccacgt ctgcaacgac catagaccag 60
ttgtgcaaga cgtttaatct atctatgcat acgtacgtcg taggttcc 108
<210> 2
<211> 108
<212> DNA
<213>artificial sequence
<400> 2
gactacatgg gacattgcta tgagcaatta gtagacagct cagaagatga ggtggacgaa 60
gtggacggac aagattcaca acctttaaaa caatacgtcg taggttcc 108
<210> 3
<211> 108
<212> DNA
<213>artificial sequence
<400> 3
gactacatgg gacattatgt tagtcctata aacactatag ccgaggcagt ggaaagtgaa 60
ataagtccac gattggacgc cattaaactt acatacgtcg taggttcc 108
<210> 4
<211> 108
<212> DNA
<213>artificial sequence
<400> 4
gactacatgg gacatgccat taagtttata tgcacatata caatggctaa caaatgcatg 60
gggaatggta ttgttagtat tattaagatt taatacgtcg taggttcc 108
<210> 5
<211> 108
<212> DNA
<213>artificial sequence
<400> 5
gactacatgg gacataacaa tggataaaac ataggggttc taaaatagaa ggcacaggaa 60
attggaaacc aattgtacaa tttctacgac atctacgtcg taggttcc 108
<210> 6
<211> 108
<212> DNA
<213>artificial sequence
<400> 6
gactacatgg gacataatcc attccctttt gacagaaatg ggaatgcagt gtatgaactg 60
tcaaatgcaa actggaaatg tttttttgaa agatacgtcg taggttcc 108
<210> 7
<211> 108
<212> DNA
<213>artificial sequence
<400> 7
gactacatgg gacatataca atggattatg tggtatggac agatgtgtat gtgcaggaca 60
ctgactcctg ggtaaaggtg catagtatgg tagtacgtcg taggttcc 108
<210> 8
<211> 108
<212> DNA
<213>artificial sequence
<400> 8
gactacatgg gacattctaa ttgtttaaag tgttttagat ataggctaaa tgacaaacac 60
agacatttat ttgatttaat atcatcaacg tggtacgtcg taggttcc 108
<210> 9
<211> 108
<212> DNA
<213>artificial sequence
<400> 9
gactacatgg gacatctaaa ggtcctgttt cgaggcggct atccatatgc agcctgcgcg 60
tgctgcctag aatttcatgg aaaaataaac caatacgtcg taggttcc 108
<210> 10
<211> 108
<212> DNA
<213>artificial sequence
<400> 10
gactacatgg gacataacat gtttagattg ggtggtagca ggatttggta tacatcatag 60
catatcagag gcatttcaaa aattaattga gcctacgtcg taggttcc 108
<210> 11
<211> 108
<212> DNA
<213>artificial sequence
<400> 11
gactacatgg gacatacaga aagcataaag cattgacatt aattaaatgt ccacctctgc 60
tagtaacgtc caacatagat attactaaag aagtacgtcg taggttcc 108
<210> 12
<211> 108
<212> DNA
<213>artificial sequence
<400> 12
gactacatgg gacatatgtg gacaatttaa aacatattat gtaaactttg taaaagaggc 60
agaaaagtat gggagcacca aacattggga agttacgtcg taggttcc 108
<210> 13
<211> 108
<212> DNA
<213>artificial sequence
<400> 13
gactacatgg gacattaccc cctaccatta gccacaaact gggatttatg tcactgcacc 60
tattgtaatt tgtatatatg taaatgtgta aattacgtcg taggttcc 108
<210> 14
<211> 108
<212> DNA
<213>artificial sequence
<400> 14
gactacatgg gacatcctaa aggttgtgtg gcgagacaac tttccctttg cagcgtgtgc 60
ctgttgctta gaactgcaag ggaaaattaa ccatacgtcg taggttcc 108
<210> 15
<211> 108
<212> DNA
<213>artificial sequence
<400> 15
gactacatgg gacatagaca tcagacaact acaagacctt ttgctgggca cactaaatat 60
tgtgtgtccc atctgcgcac caaaaccata acatacgtcg taggttcc 108
<210> 16
<211> 108
<212> DNA
<213>artificial sequence
<400> 16
gactacatgg gacattgaag tggaagctgc aacgcaggta gagaaacatg gcgacccgga 60
aaatggggga gatggtcagg aaagggacac aggtacgtcg taggttcc 108
<210> 17
<211> 108
<212> DNA
<213>artificial sequence
<400> 17
gactacatgg gacattaatt gagcctccta aaatacaaag tggcgtagca gccctgtatt 60
ggtttaggac aggcatttca aatgcaagta cagtacgtcg taggttcc 108
<210> 18
<211> 108
<212> DNA
<213>artificial sequence
<400> 18
gactacatgg gacatatagc cattgtaggg ccaccggaca ctgggaagtc gtgcttttgc 60
atgagtttaa ttaagttttt ggggggaaca gtttacgtcg taggttcc 108
<210> 19
<211> 108
<212> DNA
<213>artificial sequence
<400> 19
gactacatgg gacatcgtgc caggatcagt tgttagaact ttatgaagaa aacagtattg 60
atatacacaa acacattatg cattggaaat gcatacgtcg taggttcc 108
<210> 20
<211> 108
<212> DNA
<213>artificial sequence
<400> 20
gactacatgg gacataagta tggtagtacc aatcattggg aagtatgtta tggcagcaca 60
gttatatgtt ctcctgcatc tgtatctagc acttacgtcg taggttcc 108
<210> 21
<211> 108
<212> DNA
<213>artificial sequence
<400> 21
gactacatgg gacatgcaat ttttaaacag tgtaaaaata ccacccacca ttaggcataa 60
ggtggggttt atgtcattac atttattgta acctacgtcg taggttcc 108
<210> 22
<211> 108
<212> DNA
<213>artificial sequence
<400> 22
gactacatgg gacattaatt gagcctccta aaatacaaag tggcgtacga gccctgtatt 60
ggtttaggac aggcatttca aatgcaagta cagtacgtcg taggttcc 108
<210> 23
<211> 108
<212> DNA
<213>artificial sequence
<400> 23
gactacatgg gacatcaatt gtttaaaatg ttttagatat agactgaatg acaaatataa 60
acatttgttt gaattagcat cttcaacgtg gcatacgtcg taggttcc 108
<210> 24
<211> 108
<212> DNA
<213>artificial sequence
<400> 24
gactacatgg gacatgagta tagacattat tgttatagtt tgtatggaac aacattagaa 60
cagcaataca acaaaccgtt gtgtgatttg ttatacgtcg taggttcc 108
<210> 25
<211> 108
<212> DNA
<213>artificial sequence
<400> 25
gactacatgg gacatattgt gtgccccatc tgttctcaga aaccataatc taccatggct 60
gatcctgcag gtaccaatgg ggaagagggt acgtacgtcg taggttcc 108
<210> 26
<211> 108
<212> DNA
<213>artificial sequence
<400> 26
gactacatgg gacatacacc atgtagtcag tatagtggtg gaagtggggg tggttgcagt 60
cagtacagta gtggaagtgg gggagagggt gtttacgtcg taggttcc 108
<210> 27
<211> 108
<212> DNA
<213>artificial sequence
<400> 27
gactacatgg gacatgtata aaacaggtat atcaaatatt agtgaagtgt atggagacac 60
gccagaatgg atacaaagac aaacagtatt acatacgtcg taggttcc 108
<210> 28
<211> 108
<212> DNA
<213>artificial sequence
<400> 28
gactacatgg gacattgagt ttaatgaaat ttctgcaagg gtctgtaata tgttttgtaa 60
attctaaaag ccatttttgg ttacaaccat tagtacgtcg taggttcc 108
<210> 29
<211> 108
<212> DNA
<213>artificial sequence
<400> 29
gactacatgg gacatagacc tacgtgacca tatagactat tggaaacaca tgcgcctaga 60
atgtgctatt tattacaagg ccagagaaat gggtacgtcg taggttcc 108
<210> 30
<211> 108
<212> DNA
<213>artificial sequence
<400> 30
gactacatgg gacataggta atattatgtc ctacatctgt gtttagcagc aacgaagtat 60
cctctcctga aattattagg cagcacttgg ccatacgtcg taggttcc 108
<210> 31
<211> 108
<212> DNA
<213>artificial sequence
<400> 31
gactacatgg gacatgtgtc tactggattt atgtctatat gacaaatctt gatactgcat 60
ccacaacatt actggcgtgc tttttgcttt gcttacgtcg taggttcc 108
<210> 32
<211> 108
<212> DNA
<213>artificial sequence
<400> 32
gactacatgg gacataatta gagaattaag acattattca gactctgtgt atggagacac 60
attggaaaaa ctaactaaca ctgggttata caatacgtcg taggttcc 108
<210> 33
<211> 108
<212> DNA
<213>artificial sequence
<400> 33
gactacatgg gacatagcag acgaccttcg agcattccag cagctgtttc tgaacaccct 60
gtcctttgtg tgtccgtggt gtgcatccca gcatacgtcg taggttcc 108
<210> 34
<211> 108
<212> DNA
<213>artificial sequence
<400> 34
gactacatgg gacatgctgt tctgaagtgg aagcaacaca gattcaggta actacaaatg 60
gcgaacatgg cggcaatgta tgtagtggcg gcatacgtcg taggttcc 108
<210> 35
<211> 108
<212> DNA
<213>artificial sequence
<400> 35
gactacatgg gacatttaca cgtacctgaa acttgtatgt taattcaacc accaaaattg 60
cgaagtagtg ttgcagcact atattggtat agatacgtcg taggttcc 108
<210> 36
<211> 108
<212> DNA
<213>artificial sequence
<400> 36
gactacatgg gacataaaag gaacccccaa aaaaaattgt ttagtatttt gtggaccagc 60
aaatacagga aaatcatatt ttggaatgag ttttacgtcg taggttcc 108
<210> 37
<211> 108
<212> DNA
<213>artificial sequence
<400> 37
gactacatgg gacatccgaa ggaaaccctt tcggaacgtt taagtgcgtt gcaggacaaa 60
atcatagacc actatgaaaa tgacagtaaa gactacgtcg taggttcc 108
<210> 38
<211> 108
<212> DNA
<213>artificial sequence
<400> 38
gactacatgg gacatcacgt tttatataga atttaaaagt gaatgtgaaa aatatgggaa 60
cacaggtacg tgggaagtac attttgggaa taatacgtcg taggttcc 108
<210> 39
<211> 108
<212> DNA
<213>artificial sequence
<400> 39
gactacatgg gacataccat agtgaaacac aaagaacaaa atttttaaat actgttgcaa 60
ttccagatag tgtacaaata ttggtgggat acatacgtcg taggttcc 108
<210> 40
<211> 108
<212> DNA
<213>artificial sequence
<400> 40
gactacatgg gacatggcaa ctaatacgtt gggaaaatgc aatattcttt gcagcaaggg 60
aacatggcat acagacatta gaccaccagg tggtacgtcg taggttcc 108
<210> 41
<211> 108
<212> DNA
<213>artificial sequence
<400> 41
gactacatgg gacatagtgt gtatggaaca acattagaaa aattgacaaa caaaggtata 60
tgtgatttat taattaggtg tataacgtgt cagtacgtcg taggttcc 108
<210> 42
<211> 108
<212> DNA
<213>artificial sequence
<400> 42
gactacatgg gacatctgta actacaatgg ctgatccagc aggtacagat ggggagggga 60
cgggatgcaa tggttggttt tatgtagaag cagtacgtcg taggttcc 108
<210> 43
<211> 108
<212> DNA
<213>artificial sequence
<400> 43
gactacatgg gacatgaacg agacaatgaa actccaacac gtaatatatt gcaagtgtta 60
aaaactagca atggtaaagc tgctatgtta ggttacgtcg taggttcc 108
<210> 44
<211> 108
<212> DNA
<213>artificial sequence
<400> 44
gactacatgg gacattaatg acacaacatt tgatttgtcc caaatggtac aatgggcata 60
tgacaatgat gttatggatg atagtgaaat tgctacgtcg taggttcc 108
<210> 45
<211> 108
<212> DNA
<213>artificial sequence
<400> 45
gactacatgg gacataaata ggcatgttag atgatgctac aacgccatgt tggcattata 60
tagacaatta cctacgaaat gcactagatg gcatacgtcg taggttcc 108
<210> 46
<211> 108
<212> DNA
<213>artificial sequence
<400> 46
gactacatgg gacattatta accaccaggt ggtgccagcg ttgtcagtat caaaggccaa 60
agccttacaa gctattgaac tacaaatgat gtttacgtcg taggttcc 108
<210> 47
<211> 108
<212> DNA
<213>artificial sequence
<400> 47
gactacatgg gacatccacc acatcgaatt ccaaaacctg cgccttgggc accagtgaag 60
gtgtgcggcg ggcgacgacg tctactaagc gactacgtcg taggttcc 108
<210> 48
<211> 108
<212> DNA
<213>artificial sequence
<400> 48
gactacatgg gacatgctat gctttttgct ttgcttttgt gtgctactat ttgtgtgtct 60
tgtcatacgt ccacttgtgc tgtctgtgtc ggttacgtcg taggttcc 108
<210> 49
<211> 108
<212> DNA
<213>artificial sequence
<400> 49
gactacatgg gacatcactg ttatgagcaa ttacccgaca gctcagatga ggaggatgtc 60
atagacagtc cagctggaca agcagaaccg gactacgtcg taggttcc 108
<210> 50
<211> 108
<212> DNA
<213>artificial sequence
<400> 50
gactacatgg gacatgcaaa aaatagaata acaattgaaa aattattaga aaaattattg 60
tgtatatcta caaattgtat gttaattcag ccatacgtcg taggttcc 108
<210> 51
<211> 108
<212> DNA
<213>artificial sequence
<400> 51
gactacatgg gacataactg cacctacagg gtgtttaaaa aaacatggat atactgtaga 60
ggtgcaattt gatggtgatg tacacaacac cattacgtcg taggttcc 108
<210> 52
<211> 108
<212> DNA
<213>artificial sequence
<400> 52
gactacatgg gacatgggaa atccatttgg aatatgtaaa ctgtgtttgc ggttcttatc 60
taaaattagt gaatatagac attataatta ttctacgtcg taggttcc 108
<210> 53
<211> 108
<212> DNA
<213>artificial sequence
<400> 53
gactacatgg gacattgtca acagtacagc aagtgaccta cgaaccatac agcaactact 60
tatgggcaca gtgaatattg tgtgccctac ctgtacgtcg taggttcc 108
<210> 54
<211> 108
<212> DNA
<213>artificial sequence
<400> 54
gactacatgg gacatatggc aatactgaag tggaaactca gcagatggta caacaggtag 60
aaagtcaaaa tggcgacaca aacttaaatg acttacgtcg taggttcc 108
<210> 55
<211> 108
<212> DNA
<213>artificial sequence
<400> 55
gactacatgg gacatataga gccaccaaaa ttacggagcc aaacatgtgc attgtattgg 60
tttagaacag caatgtcaaa cattagtgat gtatacgtcg taggttcc 108
<210> 56
<211> 108
<212> DNA
<213>artificial sequence
<400> 56
gactacatgg gacatgctaa tttgtggacc agcaaataca ggaaagtcat attttggaat 60
gagtttaata cagtttttaa aagggtgtgt tattacgtcg taggttcc 108
<210> 57
<211> 108
<212> DNA
<213>artificial sequence
<400> 57
gactacatgg gacatgcagt gcaggagaaa atactagatc tttacgaagc tgataaaact 60
gatttaccat cacaaattga acattggaaa ctgtacgtcg taggttcc 108
<210> 58
<211> 108
<212> DNA
<213>artificial sequence
<400> 58
gactacatgg gacataaagt attctaaaac acaaatgtgg gaagtacatg tgggtggtca 60
ggtaattgtt tgtcctacgt ctatatctag caatacgtcg taggttcc 108
<210> 59
<211> 108
<212> DNA
<213>artificial sequence
<400> 59
gactacatgg gacatctgtg caaataagta ctggatttat gacattataa gtgtacatca 60
caagccaata tgtgctgcta attgtatata acctacgtcg taggttcc 108
<210> 60
<211> 108
<212> DNA
<213>artificial sequence
<400> 60
gactacatgg gacattggag gtcccgacgt agagaaactg cactgtgacg tgtaaaaacg 60
ccatgagagg acacgagcca acgttaaagg aattacgtcg taggttcc 108
<210> 61
<211> 108
<212> DNA
<213>artificial sequence
<400> 61
gactacatgg gacatatcaa tacctgaaac atgtatggtt atagagccac caaagttacg 60
gagccaaaca tgtgcattgt attggtttag aactacgtcg taggttcc 108
<210> 62
<211> 108
<212> DNA
<213>artificial sequence
<400> 62
gactacatgg gacatctgat acgcatggag tgtgctttat tttatacagc caaacaaatg 60
ggattttcac atttatgcca ccaggtggtg ccttacgtcg taggttcc 108
<210> 63
<211> 108
<212> DNA
<213>artificial sequence
<400> 63
gactacatgg gacatttagg tactgtaaaa ataccaccta ctgtgcaaac aagtactgga 60
tttatgacat tataagtgta catcacaagc caatacgtcg taggttcc 108
<210> 64
<211> 108
<212> DNA
<213>artificial sequence
<400> 64
gactacatgg gacatgtcaa aaaccgctgt gtccagttga aaagcaaaga catttagaag 60
aaaaaaaacg attccataac atcggtggac ggttacgtcg taggttcc 108
<210> 65
<211> 108
<212> DNA
<213>artificial sequence
<400> 65
gactacatgg gacatttgta gaagcagtag ttagtagacg tacgggggat ccagtgtcag 60
aggacgaaaa tgaagataac tgtgacaggg gggtacgtcg taggttcc 108
<210> 66
<211> 108
<212> DNA
<213>artificial sequence
<400> 66
gactacatgg gacatagtaa tgcaaacgca gctatgttgg ctaaatttaa agaactattt 60
ggtattagtt ttacagaact tattagacca ttttacgtcg taggttcc 108
<210> 67
<211> 108
<212> DNA
<213>artificial sequence
<400> 67
gactacatgg gacattgatt ttatagatga tagtgatata gcatataaat atgcacaatt 60
ggcagaaact aatagtaatg catgtgcttt ttttacgtcg taggttcc 108
<210> 68
<211> 108
<212> DNA
<213>artificial sequence
<400> 68
gactacatgg gacataatat ttaagaaatg cactagatgg aaatcctatt tcattagatg 60
taaagcataa agcattagtg caattaaaat gcctacgtcg taggttcc 108
<210> 69
<211> 108
<212> DNA
<213>artificial sequence
<400> 69
gactacatgg gacatgcaag caattgaact gcaattaatg ttagagacat taaatacaac 60
tgagtataga acagaaacat ggacactgca agatacgtcg taggttcc 108
<210> 70
<211> 108
<212> DNA
<213>artificial sequence
<400> 70
gactacatgg gacatcaaat cacaaacgac ctcgaggggg taccgagctc ccctgcaacc 60
ccaccaagcg agtgcgactc agtgccgtgg acatacgtcg taggttcc 108
<210> 71
<211> 108
<212> DNA
<213>artificial sequence
<400> 71
gactacatgg gacatcccta taaaaaaaac agggagtgac cgaaaacggt cgtaccgaaa 60
acggttgcca taaaagcaga agtgcacaaa aaatacgtcg taggttcc 108
<210> 72
<211> 108
<212> DNA
<213>artificial sequence
<400> 72
gactacatgg gacataatga agatgactgt gacagggggg aggatatggt ggactttata 60
aatgatacag atatattaaa catacaggca gaatacgtcg taggttcc 108
<210> 73
<211> 108
<212> DNA
<213>artificial sequence
<400> 73
gactacatgg gacattttta cagaacttat tagaccattt aagagtgata aatccacatg 60
tacagattgg tgtgtggccg catttggaat agctacgtcg taggttcc 108
<210> 74
<211> 108
<212> DNA
<213>artificial sequence
<400> 74
gactacatgg gacatgtgct tttttaaaaa gtaattcgca agctaaaatt gtaaaagatt 60
gtgcaacaat gtgtagacat tataaacgag ctgtacgtcg taggttcc 108
<210> 75
<211> 108
<212> DNA
<213>artificial sequence
<400> 75
gactacatgg gacatataat gcccaccttt acttattaca tcaatataaa tgcaggcaaa 60
gatgacaggt ggccatactt acatagcagg gtatacgtcg taggttcc 108
<210> 76
<211> 108
<212> DNA
<213>artificial sequence
<400> 76
gactacatgg gacattgcaa gaaacaagta ttgaactata tacaacagtt cctacaagat 60
gtttaaaaaa agatgtttat actgtggaag cactacgtcg taggttcc 108
<210> 77
<211> 108
<212> DNA
<213>artificial sequence
<400> 77
gactacatgg gacatgtgga cagtgttgac agaggggtct actctacatc tgactgcaca 60
aacaaagacc ggtgtggtag ttgtagtaca acttacgtcg taggttcc 108
<210> 78
<211> 108
<212> DNA
<213>artificial sequence
<400> 78
gactacatgg gacatcggtc aggaccgaaa tcggtggata taaaacgcag tcacagtttc 60
tgtccatacc gatggcgcga tttcacaatc ctgtacgtcg taggttcc 108
<210> 79
<211> 108
<212> DNA
<213>artificial sequence
<400> 79
gactacatgg gacatcgcag actaacacga agagaaaccc aagtataaca tcagatatgc 60
gtggaccaaa gcccaccttg caggaaattg tattacgtcg taggttcc 108
<210> 80
<211> 108
<212> DNA
<213>artificial sequence
<400> 80
gactacatgg gacatgatac aggttcagac ctggcagact ttattgatga ttccacagat 60
atttgtgtac aggcagagcg tgagacagca cagtacgtcg taggttcc 108
<210> 81
<211> 108
<212> DNA
<213>artificial sequence
<400> 81
gactacatgg gacattaaag aaacatatgg actatccttt actgacctgg tacgtacgtt 60
taaaagtgat aaaacaacat gtacagactg ggttacgtcg taggttcc 108
<210> 82
<211> 108
<212> DNA
<213>artificial sequence
<400> 82
gactacatgg gacatattat gcaatgttag cagattgtaa cagtaatgct gcagcctttt 60
taaaaagtaa ctgccaggca aaatatgtaa aagtacgtcg taggttcc 108
<210> 83
<211> 108
<212> DNA
<213>artificial sequence
<400> 83
gactacatgg gacatataag tttagatagg aaatataaaa gtttactaca aatgaaatgt 60
ccaccattat taataacctc caataccaat ccttacgtcg taggttcc 108
<210> 84
<211> 108
<212> DNA
<213>artificial sequence
<400> 84
gactacatgg gacataaagt gttgcacaaa ctgaatacaa tacagaggag tggacattaa 60
aagacactag taatgaactg tggcatacac agctacgtcg taggttcc 108
<210> 85
<211> 108
<212> DNA
<213>artificial sequence
<400> 85
gactacatgg gacattgtgc agtcacagag cccactgagc ccgacggagt gtccctggac 60
catcttaaca acccactcca cagtaacagt acatacgtcg taggttcc 108
<210> 86
<211> 108
<212> DNA
<213>artificial sequence
<400> 86
gactacatgg gacatcaacg accctacaag ctaccagatt tgtgcacaga attgaataca 60
tcactacaag acgtatctat tgcctgtgta tattacgtcg taggttcc 108
<210> 87
<211> 108
<212> DNA
<213>artificial sequence
<400> 87
gactacatgg gacatcattt ggaacctcag aatgaattag atcctgttga cctgttgtgt 60
tacgagcaat taagcgagtc agaggaggaa aactacgtcg taggttcc 108
<210> 88
<211> 108
<212> DNA
<213>artificial sequence
<400> 88
gactacatgg gacattgcgc aggaagttca gaatgatgca caggtgttgc atcttttaaa 60
acgaaagttt gcaggaggca gcaaggaaaa cagtacgtcg taggttcc 108
<210> 89
<211> 108
<212> DNA
<213>artificial sequence
<400> 89
gactacatgg gacatcaacg gtagcagaag gctttaaaac attaattaaa ccagcaacgt 60
tatacgccca tatccaatgt ttagattgta aattacgtcg taggttcc 108
<210> 90
<211> 108
<212> DNA
<213>artificial sequence
<400> 90
gactacatgg gacattataa aagagcacaa aaacgccaaa tgaatatgtc tcaatggatt 60
aaatatagat gttctaaaat agatgaaggt gggtacgtcg taggttcc 108
<210> 91
<211> 108
<212> DNA
<213>artificial sequence
<400> 91
gactacatgg gacattttag aaagtagggt gacggtattt acatttccac atgcatttcc 60
atttgataaa aatggtaatc cagtatatga aattacgtcg taggttcc 108
<210> 92
<211> 108
<212> DNA
<213>artificial sequence
<400> 92
gactacatgg gacatggtaa aaccgtgcac gtatactttg atggcaacaa ggacaactgt 60
atgaactatg tagtatggga cagtatatat tattacgtcg taggttcc 108
<210> 93
<211> 108
<212> DNA
<213>artificial sequence
<400> 93
gactacatgg gacattagtg gtaacactac gcctataata cacttaaaag gtgacaaaaa 60
cagtttgaaa tgtttaagat ataggctacg caatacgtcg taggttcc 108
<210> 94
<211> 108
<212> DNA
<213>artificial sequence
<400> 94
gactacatgg gacatcagaa accattgaac ccagcagaaa aacgtagaca ccttaaggac 60
aaacgaagat ttcacagcat agctggacag tactacgtcg taggttcc 108
<210> 95
<211> 108
<212> DNA
<213>artificial sequence
<400> 95
gactacatgg gacatcagtc cattagggga gcagctaagt gtggatacgg atctaagtcc 60
acggttacaa gaaatttcat taaatagtgg gcatacgtcg taggttcc 108
<210> 96
<211> 108
<212> DNA
<213>artificial sequence
<400> 96
gactacatgg gacatgaatt tcttaaagga acaccaaaaa aaaattgtat actgttatat 60
ggacctgcaa atacaggaaa atcgtatttt ggatacgtcg taggttcc 108
<210> 97
<211> 108
<212> DNA
<213>artificial sequence
<400> 97
gactacatgg gacatttggg gtgtatatta tataaaagat ggagatacca catattatgt 60
acaatttaaa agcgaatgtg agaaatatgg aaatacgtcg taggttcc 108
<210> 98
<211> 108
<212> DNA
<213>artificial sequence
<400> 98
gactacatgg gacataggtg ctggaagaat cggtgcatga aataaggctg cagtgtgtgc 60
agtgtaaaaa agagctacaa cgaagagagg tattacgtcg taggttcc 108
<210> 99
<211> 108
<212> DNA
<213>artificial sequence
<400> 99
gactacatgg gacattgagg aggatacaga tggtgtggac cggccggatg gacaagcaga 60
acaagccaca agcaattact acattgtgac atatacgtcg taggttcc 108
<210> 100
<211> 108
<212> DNA
<213>artificial sequence
<400> 100
gactacatgg gacattgggc aagatggtgt agaaaaacat ggtagttcgc gtgcaaaaca 60
catttgtgta aatacagagt gtgtttcacc aaatacgtcg taggttcc 108
<210> 101
<211> 108
<212> DNA
<213>artificial sequence
<400> 101
gactacatgg gacattaaca tgtgacagag gcgtgcttat actgctgcta attaggttta 60
aatgtggaaa aaacagatta acagtgtcca aactacgtcg taggttcc 108
<210> 102
<211> 108
<212> DNA
<213>artificial sequence
<400> 102
gactacatgg gacatgatga tggtggagac tggaggccta tagtgagatt tttaagatat 60
caagaaatag aatttacagc ctttttagat gcatacgtcg taggttcc 108
<210> 103
<211> 108
<212> DNA
<213>artificial sequence
<400> 103
gactacatgg gacatcatat atgaaattaa caacgaaaat tggaaatcct ttttctcaag 60
gacgtggtgc aaattagatt taatacagga agatacgtcg taggttcc 108
<210> 104
<211> 108
<212> DNA
<213>artificial sequence
<400> 104
gactacatgg gacatatata tttacttggt gagtgtgaat gtacaattgt agaaggacaa 60
gtagattatt atgggttata ttattggtgt gattacgtcg taggttcc 108
<210> 105
<211> 108
<212> DNA
<213>artificial sequence
<400> 105
gactacatgg gacataagat atagggtaaa aacacataaa ggtttgtatg ttcaaatttc 60
atctacctgg cattggacca gtaatgaatg tactacgtcg taggttcc 108
<210> 106
<211> 108
<212> DNA
<213>artificial sequence
<400> 106
gactacatgg gacatatata ggcattatca atattcactt tatgggaaaa cattagaaga 60
gagggtaaga aaaccattaa gtgaaataac tattacgtcg taggttcc 108
<210> 107
<211> 108
<212> DNA
<213>artificial sequence
<400> 107
gactacatgg gacattctaa tagattttat agatgattca aatataaata atgaacaggc 60
agaacatgag gcagcccggg cattgtttaa tgctacgtcg taggttcc 108
<210> 108
<211> 108
<212> DNA
<213>artificial sequence
<400> 108
gactacatgg gacatccacc aaaattacga agtgctacct gtgcattata ttggtataga 60
acaggtttgt ctaatattag tcaggtatat ggttacgtcg taggttcc 108
<210> 109
<211> 108
<212> DNA
<213>artificial sequence
<400> 109
gactacatgg gacataccta tatgttggac atatatagat gattatatga gaaatgcgct 60
ggatggaaat gatatatcag tagatgtaaa gcatacgtcg taggttcc 108
<210> 110
<211> 108
<212> DNA
<213>artificial sequence
<400> 110
gactacatgg gacatttgtg caacaggagt atgggaagta catgtgggtg gtcgggtaat 60
tgtttgtcct gcatctgtat ctagtaacga agttacgtcg taggttcc 108
<210> 111
<211> 108
<212> DNA
<213>artificial sequence
<400> 111
gactacatgg gacatatttg tacatatgta tatatgtata tgtgtatggt aaacacccaa 60
cacaagccaa tattgctgct attgtgtata tagtacgtcg taggttcc 108
<210> 112
<211> 108
<212> DNA
<213>artificial sequence
<400> 112
gactacatgg gacattgtca aagtccgtta actccggagg aaaagcaatt gcattgtgac 60
agaaaaagac gatttcatct aatagcacat ggttacgtcg taggttcc 108
<210> 113
<211> 108
<212> DNA
<213>artificial sequence
<400> 113
gactacatgg gacatggaag ggatgttgtg gatggtttga agtagaggca attgtagaaa 60
aaaaaacagg agataaaata tcagatgatg aaatacgtcg taggttcc 108
<210> 114
<211> 108
<212> DNA
<213>artificial sequence
<400> 114
gactacatgg gacatacaca acaattgcag gacttgttta aaagtagcaa tttacaaggt 60
aaattatatt ataaatttaa agaagtgtat ggttacgtcg taggttcc 108
<210> 115
<211> 108
<212> DNA
<213>artificial sequence
<400> 115
gactacatgg gacatatcta aaatggtgca gtgggcattt gataatgaag taacagatga 60
tagccaaatt gcgtttcaat atgcacaatt agctacgtcg taggttcc 108
<210> 116
<211> 108
<212> DNA
<213>artificial sequence
<400> 116
gactacatgg gacatcaaca gaaatatgtt ggaaatatat agacgattat ttaaggaatt 60
tggtagatgg aaatcctata agtttagata gaatacgtcg taggttcc 108
<210> 117
<211> 108
<212> DNA
<213>artificial sequence
<400> 117
gactacatgg gacatttgtt tacaagtatg taaagcaaaa gcatgtagtg caatagaagt 60
gcaaatagca ctggaatcat taagtacaac aattacgtcg taggttcc 108
<210> 118
<211> 108
<212> DNA
<213>artificial sequence
<400> 118
gactacatgg gacatcgtcc gtgggcaacc aagacgccgc agtatcccac agaccaggaa 60
aacgacccag actacgggaa tcagaatttg acttacgtcg taggttcc 108
<210> 119
<211> 108
<212> DNA
<213>artificial sequence
<400> 119
gactacatgg gacatgaaag ttttaatcat acttttatat attgggagta accgaaaagg 60
gtttaagacc gaaaacggta catataaaag gcatacgtcg taggttcc 108
<210> 120
<211> 108
<212> DNA
<213>artificial sequence
<400> 120
gactacatgg gacataattc atatatagaa aatatacagg cagacagaga gacagctcaa 60
caattgttgc aagtacaaac agcacatgca gattacgtcg taggttcc 108
<210> 121
<211> 108
<212> DNA
<213>artificial sequence
<400> 121
gactacatgg gacatctttt ggttgcagcc attagacaat gctaaacttg gtttgttgga 60
tgatgcaaca gaaatatgtt ggaaatatat agatacgtcg taggttcc 108
<210> 122
<211> 108
<212> DNA
<213>artificial sequence
<400> 122
gactacatgg gacattgaaa ctcttaacga atacaacacc cacaagacca ccaccaccac 60
ctccacgtcc gtgggcaacc aagacgccgc agttacgtcg taggttcc 108
<210> 123
<211> 108
<212> DNA
<213>artificial sequence
<400> 123
gactacatgg gacatatgga aatccatttg cagtatgtaa agtgtgttta cgattgctat 60
ctaaaataag tgagtataga cattataatt atttacgtcg taggttcc 108
<210> 124
<211> 108
<212> DNA
<213>artificial sequence
<400> 124
gactacatgg gacattgtgt atcaacagta cagcaactga agtacgaacc ctacagcagc 60
tgcttatggg cacatgtact attgtgtgcc ccatacgtcg taggttcc 108
<210> 125
<211> 108
<212> DNA
<213>artificial sequence
<400> 125
gactacatgg gacatggata tggcaatact gaagtggaaa ctgagcagat ggcacaccag 60
gtagaaagcc aaaatggcga cgcagactta aattacgtcg taggttcc 108
<210> 126
<211> 108
<212> DNA
<213>artificial sequence
<400> 126
gactacatgg gacatgatta ttgagccacc aaaattacga agtcaagcat gtgccttata 60
ttggtttaga acagcaatgt caaatataag tgatacgtcg taggttcc 108
<210> 127
<211> 108
<212> DNA
<213>artificial sequence
<400> 127
gactacatgg gacatgtatg ttactgtgtg gcccagcaaa tacagggaaa tcatattttg 60
gaatgagttt aatacatttt ttaaaaggat gcatacgtcg taggttcc 108
<210> 128
<211> 108
<212> DNA
<213>artificial sequence
<400> 128
gactacatgg gacataagtg cagtgcagga caaaatccta gacatatacg aagctgataa 60
aaatgattta acatcacaaa ttgaacattg gaatacgtcg taggttcc 108
<210> 129
<211> 108
<212> DNA
<213>artificial sequence
<400> 129
gactacatgg gacatcaaaa aagtactcta aaacacaatt atgggaggta catgtgggta 60
gtcgggtaat tgtatgtcct acatctatac ctatacgtcg taggttcc 108
<210> 130
<211> 108
<212> DNA
<213>artificial sequence
<400> 130
gactacatgg gacatactgt taaaatacca cccactgtgc aaataagtac tggtgttatg 60
tcattgtaat tgtattgtac aattactgta tgttacgtcg taggttcc 108
<210> 131
<211> 108
<212> DNA
<213>artificial sequence
<400> 131
gactacatgg gacatgatga aataggcttg gacgggccag atggacaagc acaaccggcc 60
acagctaatt actacattgt aacttgttgt tactacgtcg taggttcc 108
<210> 132
<211> 108
<212> DNA
<213>artificial sequence
<400> 132
gactacatgg gacatttcta cataacagta atactaaagc aacgctatta tataaattca 60
aagaagctta tggagtaagt tttatggaat tagtacgtcg taggttcc 108
<210> 133
<211> 108
<212> DNA
<213>artificial sequence
<400> 133
gactacatgg gacatatata tagatgatta tatgagaaat gcattagatg gtaacgacat 60
ttcaatagat gtaaaacata gggcattagt acatacgtcg taggttcc 108
<210> 134
<211> 108
<212> DNA
<213>artificial sequence
<400> 134
gactacatgg gacatgtcat ccacatggca ttggaccagt gatgacaaag gtgacaaagt 60
aggaattgtt actgtaacat acacaacgga aactacgtcg taggttcc 108
<210> 135
<211> 108
<212> DNA
<213>artificial sequence
<400> 135
gactacatgg gacattgagc tgctgatacg ctgttataga tgcctaaaac ctctatgtcc 60
aacagataaa ttaaagcata taactgaaaa aagtacgtcg taggttcc 108
<210> 136
<211> 108
<212> DNA
<213>artificial sequence
<400> 136
gactacatgg gacatgcagc aaaccagtaa cctgcaatgg ccgattcgga aggtacagat 60
ggggaaggga cggggtgcaa tggatggttt ttttacgtcg taggttcc 108
<210> 137
<211> 108
<212> DNA
<213>artificial sequence
<400> 137
gactacatgg gacatttgta gcgacagtca aatagactgt agcgacagca gtaacatgga 60
tgttgaaaac atagttccaa catcccccac taatacgtcg taggttcc 108
<210> 138
<211> 108
<212> DNA
<213>artificial sequence
<400> 138
gactacatgg gacattaggg gaaacgcccg aatggataca aagactaaca attatacaac 60
atggagttga tgatagcgtg tttgacctgt cagtacgtcg taggttcc 108
<210> 139
<211> 108
<212> DNA
<213>artificial sequence
<400> 139
gactacatgg gacattcaca tgtaaattca aatagtcact tttggctaga acctttaaca 60
gatgctaaat tagctatgct agacgatgca acatacgtcg taggttcc 108
<210> 140
<211> 108
<212> DNA
<213>artificial sequence
<400> 140
gactacatgg gacattggtg cgtatggaaa atgtaatttt atttgcagca agagagaaca 60
atatacatac attaaaccac caggtggtgc caatacgtcg taggttcc 108
<210> 141
<211> 108
<212> DNA
<213>artificial sequence
<400> 141
gactacatgg gacatagtac cagtgacgag caagtatcca cttctggatc ttctgagcaa 60
ctatcatacc cctccgcaac gccccccgaa gcctacgtcg taggttcc 108
<210> 142
<211> 108
<212> DNA
<213>artificial sequence
<400> 142
gactacatgg gacatgttgt tatgcaaatg taacacaagc caatactgct gctatattgt 60
atagctgagg aaatgataac ccttgtattt gtgtacgtcg taggttcc 108
<210> 143
<211> 108
<212> DNA
<213>artificial sequence
<400> 143
gactacatgg gacatacatt ttttacaagg aactgtaatt tcacatgtaa attcaaatag 60
tcacttttgg ctagaacctt taacagatcg taatacgtcg taggttcc 108
<210> 144
<211> 108
<212> DNA
<213>artificial sequence
<400> 144
gactacatgg gacattattg attgttatga ctctatgtgc agtaccagtg acgagcaagt 60
atccactgct ggatcttctg agcaactatc atatacgtcg taggttcc 108
<210> 145
<211> 108
<212> DNA
<213>artificial sequence
<400> 145
gactacatgg gacataacaa ttggccatat gcagtatgta gggtatgttt attgttttat 60
agtaaggtta gaaaatatag gtactataaa tattacgtcg taggttcc 108
<210> 146
<211> 108
<212> DNA
<213>artificial sequence
<400> 146
gactacatgg gacatgagtt ggtggtgcag ttggacattc agagtaccaa agaggagcta 60
cgtgtggtac aacagctgct tatgggtgcg ttatacgtcg taggttcc 108
<210> 147
<211> 108
<212> DNA
<213>artificial sequence
<400> 147
gactacatgg gacatgaaac atcacaacag gtagaatacg aaaagggaaa tgggtgcggg 60
agctcacaaa atggaggctc gcaaaacagt aattacgtcg taggttcc 108
<210> 148
<211> 108
<212> DNA
<213>artificial sequence
<400> 148
gactacatgg gacataagtc ctgctgtagc attatatttt tataaaacag caatgtcaaa 60
tattagtgag gtgtatgggg aaacaccaga atgtacgtcg taggttcc 108
<210> 149
<211> 108
<212> DNA
<213>artificial sequence
<400> 149
gactacatgg gacatataca ggtaaatcat gttttgctat gagccttata aattttttcc 60
aagggtcagt catttcattt gttaattcac aaatacgtcg taggttcc 108
<210> 150
<211> 108
<212> DNA
<213>artificial sequence
<400> 150
gactacatgg gacatagact gttatgaaaa agatagtaaa tgcattatag atcacataga 60
ctattggaaa gctgtacgac atgaatatgt atttacgtcg taggttcc 108
<210> 151
<211> 108
<212> DNA
<213>artificial sequence
<400> 151
gactacatgg gacattatgg gaagtacata tggaaaccga gagtatttac tgtcctgact 60
ctgtgtctag tacctgtaga tacaacgtac ccctacgtcg taggttcc 108
<210> 152
<211> 108
<212> DNA
<213>artificial sequence
<400> 152
gactacatgg gacatttaaa tgttgtaaaa ataccaccta gtgtacaggt tattttggga 60
caaatgagtt gtccataaag tgttgtatat atttacgtcg taggttcc 108
<210> 153
<211> 108
<212> DNA
<213>artificial sequence
<400> 153
gactacatgg gacatgatta tattagaact tgcaccgcaa actgaaattg acctacaatg 60
caatgagcaa ttggacagct cagaggatga ggatacgtcg taggttcc 108
<210> 154
<211> 108
<212> DNA
<213>artificial sequence
<400> 154
gactacatgg gacataaaag ggaaatgggt gcgggagctc acaaaatgga ggctcgcaaa 60
acagtaactg tagtgagcac tcggtggtaa atatacgtcg taggttcc 108
<210> 155
<211> 108
<212> DNA
<213>artificial sequence
<400> 155
gactacatgg gacatcgata tcaaggggtc gacttcattt catttttaag ttattttaaa 60
ttatttttac aaggcacgcc taaacataat tgttacgtcg taggttcc 108
<210> 156
<211> 108
<212> DNA
<213>artificial sequence
<400> 156
gactacatgg gacatgccaa aaaatatggg tgtacaaaca tatgggaagt acatatggaa 60
accgagagta tttattgtcc tgactctgtg tcttacgtcg taggttcc 108
<210> 157
<211> 108
<212> DNA
<213>artificial sequence
<400> 157
gactacatgg gacatatggc gctatttcac aaccctgagg aacggccata caaattgcca 60
gacctgtgca ggacattgga caccacattg cattacgtcg taggttcc 108
<210> 158
<211> 108
<212> DNA
<213>artificial sequence
<400> 158
gactacatgg gacatccacc gtgcaggaaa ttgtgttaga gttatgtcca tgcaatgaaa 60
tagagccggt tgaccttgta tgtcacgagc aattacgtcg taggttcc 108
<210> 159
<211> 108
<212> DNA
<213>artificial sequence
<400> 159
gactacatgg gacatcaggc agagcgtgag acagcacaag tactgttaaa tatgcaacag 60
gcccaaaggg atgcacaaac agtgcgtgcc ctatacgtcg taggttcc 108
<210> 160
<211> 108
<212> DNA
<213>artificial sequence
<400> 160
gactacatgg gacatggtag cagcaatatt tggagtaaat ccaaccattg ccgaagggtt 60
taaaacacta attaaacaat atgcattata tactacgtcg taggttcc 108
<210> 161
<211> 108
<212> DNA
<213>artificial sequence
<400> 161
gactacatgg gacataagat tgtgcaacaa tgtgtagaca ttacaaacgg gcacaaaaac 60
gacaaatgtc aatgccgcaa tggattaaat ttatacgtcg taggttcc 108
<210> 162
<211> 108
<212> DNA
<213>artificial sequence
<400> 162
gactacatgg gacatcctgt ggaagacaat aggtggcctt atttacatag cggactaact 60
gtgtttaaat ttcctaatgc atttccattt gactacgtcg taggttcc 108
<210> 163
<211> 108
<212> DNA
<213>artificial sequence
<400> 163
gactacatgg gacatagcca aagcaatgtt ttaaaaaaca tggtgttaca gtggaagtat 60
ggtatgacgg tgacaaaagt aactcaatgc acttacgtcg taggttcc 108
<210> 164
<211> 108
<212> DNA
<213>artificial sequence
<400> 164
gactacatgg gacatgcagg ccacaacaaa agaaggaacg tgtgttgtgg tgacactaca 60
cctatagtgc atttaaaagg tgacaaaaat ggttacgtcg taggttcc 108
<210> 165
<211> 108
<212> DNA
<213>artificial sequence
<400> 165
gactacatgg gacatcatga cgttacaata gactgtgtct attgcagaag gcaactacaa 60
cggacagagg tatatgaatt tgcctttagt gactacgtcg taggttcc 108
<210> 166
<211> 108
<212> DNA
<213>artificial sequence
<400> 166
gactacatgg gacatattag gagattcaga cgatgaaata gatgaacccg accatgcagt 60
taatcaccac caacatctac tactagccag acgtacgtcg taggttcc 108
<210> 167
<211> 108
<212> DNA
<213>artificial sequence
<400> 167
gactacatgg gacattaaaa cgaaagtata cagacagtat agaaagcagt cctttagcaa 60
agtcgccatt acaggaatta tcactaaatg taatacgtcg taggttcc 108
<210> 168
<211> 108
<212> DNA
<213>artificial sequence
<400> 168
gactacatgg gacataccca tatacaatgt ttagatacaa aaactggaat attaatacta 60
atgctaataa gatacaaatg tggaaaaaat agatacgtcg taggttcc 108
<210> 169
<211> 108
<212> DNA
<213>artificial sequence
<400> 169
gactacatgg gacattagat gcagtaaatg tgacgaaggc ggtgattggc gcccaattgt 60
acaattttta agatatcaag gactggaatt tattacgtcg taggttcc 108
<210> 170
<211> 108
<212> DNA
<213>artificial sequence
<400> 170
gactacatgg gacataccaa aacaggaacc cagtgtatac aatcaatgat aaaaactgga 60
aatgtttttt tgaaaagact tggtgcagat tagtacgtcg taggttcc 108
<210> 171
<211> 108
<212> DNA
<213>artificial sequence
<400> 171
gactacatgg gacatttatg tagtgtgggg tacaatttac tttaaaaaca gtacagacac 60
atggtgtaaa acccaagggc gtgtggatta ctgtacgtcg taggttcc 108
<210> 172
<211> 108
<212> DNA
<213>artificial sequence
<400> 172
gactacatgg gacatgatta aaatgtctta ggtataggtt gcaaaaacat aacgctttgt 60
atgacaatat atcatgtaca tggcattgga taatacgtcg taggttcc 108
<210> 173
<211> 108
<212> DNA
<213>artificial sequence
<400> 173
gactacatgg gacataagct ttgaatacac ctttgcaatc tttgcaggta cagtgtgtat 60
attgcaagaa aacattagaa tgggcagatg tattacgtcg taggttcc 108
<210> 174
<211> 108
<212> DNA
<213>artificial sequence
<400> 174
gactacatgg gacatgacta tgaacaattt gacagctcag aggaggatga aacagataat 60
gtgcgtaacc agcaagccag acaagctgaa caatacgtcg taggttcc 108
<210> 175
<211> 108
<212> DNA
<213>artificial sequence
<400> 175
gactacatgg gacatgtagt cagaacagcc cgttgcaaga cataacaaat caaagcaaca 60
gtcagcagag cacagacgaa gtaaacaatt tactacgtcg taggttcc 108
<210> 176
<211> 108
<212> DNA
<213>artificial sequence
<400> 176
gactacatgg gacataattg gggagtacta ccactaatgc taatacgatt tacatgtgcc 60
aaaaatagag caacaattaa aaaatgcctg tgttacgtcg taggttcc 108
<210> 177
<211> 108
<212> DNA
<213>artificial sequence
<400> 177
gactacatgg gacataggaa cgtggaagga catagcaaga tttctaagat atcaaaatgt 60
taattttatt tattttttgc aagtattaaa acatacgtcg taggttcc 108
<210> 178
<211> 108
<212> DNA
<213>artificial sequence
<400> 178
gactacatgg gacataattg actgatgtaa attggaaaag ttttttttcc accacttggt 60
ccagattaga tttggaggag gacgcggaca aagtacgtcg taggttcc 108
<210> 179
<211> 108
<212> DNA
<213>artificial sequence
<400> 179
gactacatgg gacataacag atatgggatg gtgtaaaaca aatggagaag tggattataa 60
ggggatatac tatatgcatg ggtgttgtaa acatacgtcg taggttcc 108
<210> 180
<211> 108
<212> DNA
<213>artificial sequence
<400> 180
gactacatgg gacattaagg tatagattta aaaaacataa gggattgtat tgtaaagtgt 60
catctacctg gcattggacc agtaatacta acctacgtcg taggttcc 108
<210> 181
<211> 108
<212> DNA
<213>artificial sequence
<400> 181
gactacatgg gacattacag aaggtatagt aggtctgtgt atggtgctac attagaggcc 60
attactaaca aaagtttata tgaattatta atatacgtcg taggttcc 108
<210> 182
<211> 108
<212> DNA
<213>artificial sequence
<400> 182
gactacatgg gacatgtgac ctaagcctgg tgggcccgtg ttgcgcgaac aactaacaac 60
ggcgatggac agtgaaggta cagatgagga gggtacgtcg taggttcc 108
<210> 183
<211> 108
<212> DNA
<213>artificial sequence
<400> 183
gactacatgg gacataaaat ggcgggtcac aacacagtat gtgtagtggc ggggggagca 60
gcgacagaag tacagacata gaccttgaaa caatacgtcg taggttcc 108
<210> 184
<211> 108
<212> DNA
<213>artificial sequence
<400> 184
gactacatgg gacatggaat atctaatatt agtactacat atggagaaac accagaatgg 60
attacaagac aaacacaact acaacatagt ttttacgtcg taggttcc 108
<210> 185
<211> 108
<212> DNA
<213>artificial sequence
<400> 185
gactacatgg gacattagat ttttgcaggg gtgtattata tcatatgtaa attcgggcag 60
ccatttctgg ttacagccac tagaggatgc taatacgtcg taggttcc 108
<210> 186
<211> 108
<212> DNA
<213>artificial sequence
<400> 186
gactacatgg gacatcaaat aaactattgg acattggtac gatacgaatg tgctatgttt 60
tatacagcac gggaaaaaaa cttacaaact ctttacgtcg taggttcc 108
<210> 187
<211> 108
<212> DNA
<213>artificial sequence
<400> 187
gactacatgg gacattgaat atgtatctag tacctgcaga gacccgttac cctctactac 60
aactgttgaa cagcactcga acacccccac gactacgtcg taggttcc 108
<210> 188
<211> 108
<212> DNA
<213>artificial sequence
<400> 188
gactacatgg gacattgtat tgtacatata tatataggta atacaagcca atatgtgctg 60
ctaattgtat atattgaatt gcaaccattg cggtacgtcg taggttcc 108
<210> 189
<211> 108
<212> DNA
<213>artificial sequence
<400> 189
gactacatgg gacattaatg ttttattcta gaattagaga atacagaagg tatagtaggt 60
ctgtgtatgg tgctacatta gaggccatta ctatacgtcg taggttcc 108
<210> 190
<211> 108
<212> DNA
<213>artificial sequence
<400> 190
gactacatgg gacattcgca tatttcagca aatgttactg ggcgacctaa gcctggtgtg 60
cccgtggtgt gcgaccaact aacatcggca atgtacgtcg taggttcc 108
<210> 191
<211> 108
<212> DNA
<213>artificial sequence
<400> 191
gactacatgg gacatggaag gacccttaca ggtagatggg caaaatgacg ggtcacaaca 60
tagtatgtgt agtggcgggg ggagcagcga cagtacgtcg taggttcc 108
<210> 192
<211> 108
<212> DNA
<213>artificial sequence
<400> 192
gactacatgg gacatcagcc gttgcattgt acttttatag aacaggaata tcaaacatta 60
gtagcacata tggcgaaaca ccagaatgga ttatacgtcg taggttcc 108
<210> 193
<211> 108
<212> DNA
<213>artificial sequence
<400> 193
gactacatgg gacatgggaa atcacaattt gcaatgagtt taattagatt tttgcaaggg 60
tgcgttattt catatgtaaa ttcaggtagt cattacgtcg taggttcc 108
<210> 194
<211> 108
<212> DNA
<213>artificial sequence
<400> 194
gactacatgg gacatatgaa ctggacagtg ataaattagt agatcaaatt aattattgga 60
cgttggtacg atatgaatgt gctatgtttt atatacgtcg taggttcc 108
<210> 195
<211> 108
<212> DNA
<213>artificial sequence
<400> 195
gactacatgg gacattatat gtgtggcaat gtaataacat gtcctgaata tgtatctagt 60
acctacagca ccccgtcacc ctctactaca acttacgtcg taggttcc 108
<210> 196
<211> 108
<212> DNA
<213>artificial sequence
<400> 196
gactacatgg gacatgtcat tgggtattat gacagtgtaa atatatgtat tgtacatata 60
taggtttaca agccaatatg tgctgctaag tgttacgtcg taggttcc 108
<210> 197
<211> 108
<212> DNA
<213>artificial sequence
<400> 197
gactacatgg gacataaaag cttatatgat ttatcgataa ggtgtcatag atgtcaaaga 60
ccacttgggc ctgaagaaaa gcaaaaattg gtgtacgtcg taggttcc 108
<210> 198
<211> 108
<212> DNA
<213>artificial sequence
<400> 198
gactacatgg gacatggact gtgaaggtac agaggatgag ggggcggggt gtaatgggtg 60
gttttttgtt gaagcaatag tagaaaaaaa aactacgtcg taggttcc 108
<210> 199
<211> 108
<212> DNA
<213>artificial sequence
<400> 199
gactacatgg gacatcaaca gaaagctgtg caaatgtaga actaaacagt atatgtgaag 60
tattaaaaag cagtaatgca aaagcaacgt taatacgtcg taggttcc 108
<210> 200
<211> 108
<212> DNA
<213>artificial sequence
<400> 200
gactacatgg gacatagttt tgaggatagt acctttgaat tatcacaaat ggtgcaatgg 60
gcatttgacc atgaagtatt agatgatagt gaatacgtcg taggttcc 108
<210> 201
<211> 108
<212> DNA
<213>artificial sequence
<400> 201
gactacatgg gacattgcta aaatagcatt gttagatgat gctacgtatg ggtgttggac 60
atatattgat cagtatttaa gaaacttttt agatacgtcg taggttcc 108
<210> 202
<211> 108
<212> DNA
<213>artificial sequence
<400> 202
gactacatgg gacatacaat caatcaccag gtagtaccag caacaacagt atcaaaacaa 60
aaggcctgtc aagcaattga aatgcacatg gcctacgtcg taggttcc 108
<210> 203
<211> 108
<212> DNA
<213>artificial sequence
<400> 203
gactacatgg gacataacga ccaatcccct taccacctgc gtgggcgcca aagaagccca 60
gacacaacag cgaaaacgac agcgacttac tgatacgtcg taggttcc 108
<210> 204
<211> 108
<212> DNA
<213>artificial sequence
<400> 204
gactacatgg gacatttgca gtgtttatta ttttgctatt tgtgctttgc ttgtgtgtgt 60
gtcttgtgtt gtgttgtttg ttgccgctac tgctacgtcg taggttcc 108
<210> 205
<211> 108
<212> DNA
<213>artificial sequence
<400> 205
gactacatgg gacatggttc catgaaatag cgggacgttg gacggggcaa tgcgctaatt 60
gctggcaacg tacacgacaa cgtaacgaaa ccctacgtcg taggttcc 108
<210> 206
<211> 108
<212> DNA
<213>artificial sequence
<400> 206
gactacatgg gacattcgga tgatgaggat gaaaatgcag atgatacagg atctgattta 60
ataaacttta tagatagtga aactagtatt tgctacgtcg taggttcc 108
<210> 207
<211> 108
<212> DNA
<213>artificial sequence
<400> 207
gactacatgg gacatagagt tgtatggtat tagttataat gagttggtac gggtgtttaa 60
aagtgataaa acatgttgta tagattgggt ttgtacgtcg taggttcc 108
<210> 208
<211> 108
<212> DNA
<213>artificial sequence
<400> 208
gactacatgg gacatatgca caattagcag atatagatag taatgctgca gcgtttttaa 60
agagtaattg ccaagcaaaa tatgtaaaag atttacgtcg taggttcc 108
<210> 209
<211> 108
<212> DNA
<213>artificial sequence
<400> 209
gactacatgg gacatagtat agatagaaaa cataggagtt taatacaatt agtatgtcca 60
ccattactaa taacgtcaaa cataaatcca caatacgtcg taggttcc 108
<210> 210
<211> 108
<212> DNA
<213>artificial sequence
<400> 210
gactacatgg gacatacaaa tcagactata acatggaacc atggacaatg cgggagacat 60
gttatgaact atggtgtgtg gctcccaagc aattacgtcg taggttcc 108
<210> 211
<211> 108
<212> DNA
<213>artificial sequence
<400> 211
gactacatgg gacatacaat ctccccactg tccgtggaca atacaaacaa ccaaatacac 60
tgtggaagtg gaagcactaa cactggaggg cactacgtcg taggttcc 108
<210> 212
<211> 108
<212> DNA
<213>artificial sequence
<400> 212
gactacatgg gacattgtaa ccgaaaacgg ttgcaaccga aaccggtaca tataaaagta 60
aaaggctagc tacgtgcaaa acagctatgt tcgtacgtcg taggttcc 108
<210> 213
<211> 108
<212> DNA
<213>artificial sequence
<400> 213
gactacatgg gacatcgcca aacagaaaca caagtgtaaa gaacaatgca tggaaacata 60
attaatattg aagatgtaat actagatctg gtgtacgtcg taggttcc 108
<210> 214
<211> 108
<212> DNA
<213>artificial sequence
<400> 214
gactacatgg gacattagat gatagtagta taagtgatta tgcagaacag gaggtaaccc 60
aggcattgtt tcaggcacaa caaaaacagg caatacgtcg taggttcc 108
<210> 215
<211> 108
<212> DNA
<213>artificial sequence
<400> 215
gactacatgg gacataaaag tgacaaaaca tgctgttcag attgggtgtg tgcagcattc 60
ggtgtggcag gctctgtagc agaaagtatt aaatacgtcg taggttcc 108
<210> 216
<211> 108
<212> DNA
<213>artificial sequence
<400> 216
gactacatgg gacataaaaa gcaattgtca ggcaaaatat gtaaaagact gtgcaaccat 60
gactagacat tataaaagag cacagaaacg atctacgtcg taggttcc 108
<210> 217
<211> 108
<212> DNA
<213>artificial sequence
<400> 217
gactacatgg gacatcccca ttaataatta cctcaaatat aaatcctcaa gaagataact 60
cacttttgta tttacatagt agagtaacag tgatacgtcg taggttcc 108
<210> 218
<211> 108
<212> DNA
<213>artificial sequence
<400> 218
gactacatgg gacataagct atgaaatgta tatgacagaa cctaaacatt gttttaaaaa 60
agaaggaaca acggtaacag tggtatttga ttgtacgtcg taggttcc 108
<210> 219
<211> 108
<212> DNA
<213>artificial sequence
<400> 219
gactacatgg gacatccaca cagcccggac agtcagtgga ctatacaaac aacaacctac 60
acagtacaag tggaggccat cacccgggaa gggtacgtcg taggttcc 108
<210> 220
<211> 108
<212> DNA
<213>artificial sequence
<400> 220
gactacatgg gacataaaat acaggcgagc gcccacgtac tgtgcaccat ctttgtgagg 60
tacaagaaac atcgttgctg gagctacagc tactacgtcg taggttcc 108
<210> 221
<211> 108
<212> DNA
<213>artificial sequence
<400> 221
gactacatgg gacataaact gagattgacc tgcattgcta tgagcaattg aacagctcag 60
aggaagagga tgaggatgaa gtagacaatt tactacgtcg taggttcc 108
<210> 222
<211> 108
<212> DNA
<213>artificial sequence
<400> 222
gactacatgg gacatgcaga tacacagacg ctgcacaatt taaaacgaaa gtatttaggc 60
agtccgttgg gagacattag taatcagcaa ttttacgtcg taggttcc 108
<210> 223
<211> 108
<212> DNA
<213>artificial sequence
<400> 223
gactacatgg gacatttacc atatacagtg tttaacgtgt acatggggcg ttgtaatatt 60
aatgttaatt agatatacat gtggaaaaaa tagtacgtcg taggttcc 108
<210> 224
<211> 108
<212> DNA
<213>artificial sequence
<400> 224
gactacatgg gacatacata tgtagtaaag tagatgaggg cggggactgg aggccaattg 60
tacagtttct aagatatcaa ggggttgact ttatacgtcg taggttcc 108
<210> 225
<211> 108
<212> DNA
<213>artificial sequence
<400> 225
gactacatgg gacatgatac aaatggtaat cctgtatatc aattaaataa tgtaaactgg 60
aaatgttttt ttgaaaggac atggtccaga ttatacgtcg taggttcc 108
<210> 226
<211> 108
<212> DNA
<213>artificial sequence
<400> 226
gactacatgg gacatatgtt gtgtggcaat gggtgtatta ctgtggggac aatgggtgga 60
ctaaagtgcc ttctgtagta gattacaaag gtatacgtcg taggttcc 108
<210> 227
<211> 108
<212> DNA
<213>artificial sequence
<400> 227
gactacatgg gacatgtgca tttaaaaggt gaaccaaaca gattaaagtg tttaagatat 60
aggtgtcaaa aacataagca cctatttgta aattacgtcg taggttcc 108
<210> 228
<211> 108
<212> DNA
<213>artificial sequence
<400> 228
gactacatgg gacatgttgc tggagctaca gctacagtgt gtatattgca agaaggaatt 60
atccagctca gaggtatata attttgcatg taatacgtcg taggttcc 108
<210> 229
<211> 108
<212> DNA
<213>artificial sequence
<400> 229
gactacatgg gacatggatg aagtagacca tttacagaag cagccacagc aagctagaca 60
agaagaacaa catccttgtt acctaattaa cactacgtcg taggttcc 108
<210> 230
<211> 108
<212> DNA
<213>artificial sequence
<400> 230
gactacatgg gacatacatt agtaatcagc aatctgtgtg ccgggaagga gtaaaacgaa 60
ggataattga tacagacgtg gccgacagcg ggttacgtcg taggttcc 108
<210> 231
<211> 108
<212> DNA
<213>artificial sequence
<400> 231
gactacatgg gacattatac atgtggaaaa aatagaaaaa caattataaa atcactaagt 60
tccattgtaa atgtgcctag cgagcaaatg ctttacgtcg taggttcc 108
<210> 232
<211> 108
<212> DNA
<213>artificial sequence
<400> 232
gactacatgg gacatatatc aaggggtcga cttcatttca tttttaagtt attttaaatt 60
atttttacaa ggcacaccaa aacacaactg ccttacgtcg taggttcc 108
<210> 233
<211> 108
<212> DNA
<213>artificial sequence
<400> 233
gactacatgg gacataaagg acatggtcca gattagattt gaacaacgac gaagacaagg 60
aaaaccatgg agacgctatg ccaacgttta gattacgtcg taggttcc 108
<210> 234
<211> 108
<212> DNA
<213>artificial sequence
<400> 234
gactacatgg gacattgtag tagattacaa aggtatatat tatgtacatg acgggaacaa 60
agtatattat acagacttta aggacgaggc atttacgtcg taggttcc 108
<210> 235
<211> 108
<212> DNA
<213>artificial sequence
<400> 235
gactacatgg gacatagaaa cataagcacc tatttgtaaa tatatcgtct acatatcatt 60
ggaccaatac acatacagag tacagctaca ttatacgtcg taggttcc 108
<210> 236
<211> 108
<212> DNA
<213>artificial sequence
<400> 236
gactacatgg gacattttat gtgttgtata tagaaagggg aaaccacttg gggtatgtca 60
accgtgttta ttgttttact caaaggttag acatacgtcg taggttcc 108
<210> 237
<211> 108
<212> DNA
<213>artificial sequence
<400> 237
gactacatgg gacatcattg agagcacaca cgctgaccta ttagtgttag aagacctgct 60
tatgggtgca ctaaaaattg tgtgccccaa ctgtacgtcg taggttcc 108
<210> 238
<211> 108
<212> DNA
<213>artificial sequence
<400> 238
gactacatgg gacatgcaat actgaagtgg aaacgtatga gagacaggta ccggggccgg 60
ggggatgttt acaatgtaat agcagtagcg acatacgtcg taggttcc 108
<210> 239
<211> 108
<212> DNA
<213>artificial sequence
<400> 239
gactacatgg gacatgaata tatgttaata gaaccaccta gactaagaag cacaccatgt 60
gcattatact ggtacaaaac aagcttatca aactacgtcg taggttcc 108
<210> 240
<211> 108
<212> DNA
<213>artificial sequence
<400> 240
gactacatgg gacatacaaa attgtatagt tatatatgga ccaccagata caggaaagtc 60
acattttgga atgagtttaa tgaattttat gcatacgtcg taggttcc 108
<210> 241
<211> 108
<212> DNA
<213>artificial sequence
<400> 241
gactacatgg gacataaacg tttaagtgcg tgccaggacg caatcctaga actgtatgaa 60
cgtgatagta tacatttaag tgatcatatt gattacgtcg taggttcc 108
<210> 242
<211> 108
<212> DNA
<213>artificial sequence
<400> 242
gactacatgg gacattagct cagatgcaaa acggtatggg gttaaaggaa tatgggatgt 60
atgtatgggc ggtgaggtaa tatgttctgc tcctacgtcg taggttcc 108
<210> 243
<211> 108
<212> DNA
<213>artificial sequence
<400> 243
gactacatgg gacatcaggg tatatgtcct tataacacat aaatgtaaca ttggttacaa 60
ttgtaaaaca tttcattgta tactatactt gtatacgtcg taggttcc 108
<210> 244
<211> 108
<212> DNA
<213>artificial sequence
<400> 244
gactacatgg gacataccat tgctggagct gcaacttggc tgtgtgttct gcaagaaggc 60
attgacagcg tcagaggtat ataattttgc atatacgtcg taggttcc 108
<210> 245
<211> 108
<212> DNA
<213>artificial sequence
<400> 245
gactacatgg gacattgagg atgaagtaga ccatctgcag gagcagccac agcaagctag 60
acgggacgaa caacatcctt gttacctaat tgatacgtcg taggttcc 108
<210> 246
<211> 108
<212> DNA
<213>artificial sequence
<400> 246
gactacatgg gacatagtaa tcagcaaaca gtgtgccggg aagctgtaaa acgaaggcta 60
attgatacag aagtgccgga cagcgggtat ggctacgtcg taggttcc 108
<210> 247
<211> 108
<212> DNA
<213>artificial sequence
<400> 247
gactacatgg gacattattg ttaattagat atacgtgtgg caaaaacaga aaaacaattg 60
taaagtcttt agcatccata ttaaatgtac caatacgtcg taggttcc 108
<210> 248
<211> 108
<212> DNA
<213>artificial sequence
<400> 248
gactacatgg gacatgtaca gttcctaagg tatcaagggg tcgagtttat ttcattttta 60
agttatttta aattattttt acaaggtaca ccatacgtcg taggttcc 108
<210> 249
<211> 108
<212> DNA
<213>artificial sequence
<400> 249
gactacatgg gacatgaaat gtttttttga aaggacatgg tccagattag atttggataa 60
cgacgaagac aaggaaaacg atggagacgc tattacgtcg taggttcc 108
<210> 250
<211> 108
<212> DNA
<213>artificial sequence
<400> 250
gactacatgg gacattgtaa agtgtcttct gcagtaagct atgagggcat atattatata 60
catgacggcc ataaaacgta ttatacaaac ttttacgtcg taggttcc 108
<210> 251
<211> 108
<212> DNA
<213>artificial sequence
<400> 251
gactacatgg gacatcagac taaagtgttt aaggtatcgg tttcaaaaac ataaacaact 60
gtttgttact gtatcatcca cctatcattg gactacgtcg taggttcc 108
<210> 252
<211> 108
<212> DNA
<213>artificial sequence
<400> 252
gactacatgg gacatgaaaa tagtatacag atgtgggaat ccgtatgggg tatgtaagca 60
atgcctaaga ctgttatcaa aagttagtga atatacgtcg taggttcc 108
<210> 253
<211> 108
<212> DNA
<213>artificial sequence
<400> 253
gactacatgg gacattgagt gcactttgcg tttgtgcatt cacagtacag cagcagacgt 60
ccgaacaatc cagcagatgc ttatgaacac acttacgtcg taggttcc 108
<210> 254
<211> 108
<212> DNA
<213>artificial sequence
<400> 254
gactacatgg gacataagtg gaaactcaag agacaatggt gcaggtagag gggcaaaatg 60
gcgatatgca gtgcagtagt cagtgtagta cggtacgtcg taggttcc 108
<210> 255
<211> 108
<212> DNA
<213>artificial sequence
<400> 255
gactacatgg gacatgaacc accaaaaata cggagtacaa cgtgtgcact atattggttt 60
agaacaggaa tgtcaaatat aagtgaagta agttacgtcg taggttcc 108
<210> 256
<211> 108
<212> DNA
<213>artificial sequence
<400> 256
gactacatgg gacataatat gcggaccacc aaacacaggg aaaacatatt ttgcaatgag 60
tttaatccac tttttacaag gatgtgtaat atctacgtcg taggttcc 108
<210> 257
<211> 108
<212> DNA
<213>artificial sequence
<400> 257
gactacatgg gacatgtgca ggagaaaatt ctagatgtat acgaagctaa taaaaaagac 60
ttatgtacac aaattgaaca ttggagactg agatacgtcg taggttcc 108
<210> 258
<211> 108
<212> DNA
<213>artificial sequence
<400> 258
gactacatgg gacatgtatt caaaacaaaa catttgggaa gtgcatgtgg gtgctcaggt 60
aattgtttgt cctacatcta tgtttagtaa cgatacgtcg taggttcc 108
<210> 259
<211> 108
<212> DNA
<213>artificial sequence
<400> 259
gactacatgg gacatcacaa cgacaacagt ttttaaactc tgtacgcata ccccccactg 60
tgcaaattgt ttgtggtata atgtcattgt aattacgtcg taggttcc 108
<210> 260
<211> 108
<212> DNA
<213>artificial sequence
<400> 260
gactacatgg gacatccatt gtgtccacaa gaaaagcaaa ggcatgtcga tcgcaaaaag 60
cgatttcata atatttccaa tcgttggaca ggatacgtcg taggttcc 108
<210> 261
<211> 108
<212> DNA
<213>artificial sequence
<400> 261
gactacatgg gacatgtgta acgtttacac agctaataag accatttaaa agcgacagga 60
caagctgtac agattggtgt gttacagcgt ttgtacgtcg taggttcc 108
<210> 262
<211> 108
<212> DNA
<213>artificial sequence
<400> 262
gactacatgg gacataaaac atattttgca atgagtttaa ttcactttct aaaaggatgt 60
gtaatatcat atgtaaactc taaaagtcat ttttacgtcg taggttcc 108
<210> 263
<211> 108
<212> DNA
<213>artificial sequence
<400> 263
gactacatgg gacattatga aacagaaaaa acgtattatg tacagtttga aaccgatgct 60
aaaaagtatt caaaacaaaa catttgggaa gtgtacgtcg taggttcc 108
<210> 264
<211> 108
<212> DNA
<213>artificial sequence
<400> 264
gactacatgg gacatgtgca aattgtttgt ggtataatgt cattgtaata tattgtatat 60
tactacatta cacacaagcc aatatgtgct gcttacgtcg taggttcc 108
<210> 265
<211> 108
<212> DNA
<213>artificial sequence
<400> 265
gactacatgg gacatggaaa gcataactaa taccaagtta tataatttat caataaggtg 60
catgagttgc ctgaaaccat tgtgtccagc agatacgtcg taggttcc 108
<210> 266
<211> 108
<212> DNA
<213>artificial sequence
<400> 266
gactacatgg gacatgacac tgtcatttgt gtgtccctgg tgtgcatcgg gaacccagta 60
acctgcaatg gccaattgtg aaggtacaga tggtacgtcg taggttcc 108
<210> 267
<211> 108
<212> DNA
<213>artificial sequence
<400> 267
gactacatgg gacatctgta gtaaataata caaatgggga agaggaaggg gaaaatggcg 60
gggaaaatgg cggcagcata cgggaggagt gcatacgtcg taggttcc 108
<210> 268
<211> 108
<212> DNA
<213>artificial sequence
<400> 268
gactacatgg gacatcctgt tgcagcactg tattggtata gaactggaat gtctaatata 60
agtgaagtgt caggtactac gccagaatgg atatacgtcg taggttcc 108
<210> 269
<211> 108
<212> DNA
<213>artificial sequence
<400> 269
gactacatgg gacataggca agtcatactt ttgtatgagt ttaatgcact ttttacaagg 60
tacagtaatt tcatatgtaa attccactag tcatacgtcg taggttcc 108
<210> 270
<211> 108
<212> DNA
<213>artificial sequence
<400> 270
gactacatgg gacatgaaca ttatgaacag gacagtaaac taatatatga tcaaatcaat 60
tattggaaat atgtgcgact ggaaaatgca atatacgtcg taggttcc 108
<210> 271
<211> 108
<212> DNA
<213>artificial sequence
<400> 271
gactacatgg gacataaaat gggaagtgca ttgtaatggc aacataattc attgtcctga 60
ctctatgtac agtaccagtg acgacacagt acctacgtcg taggttcc 108
<210> 272
<211> 108
<212> DNA
<213>artificial sequence
<400> 272
gactacatgg gacattggga tatatgacat tgtaacagca catgctgtat gtatattgta 60
tacatatcaa tgattgcatt ggtgtttttg gtgtacgtcg taggttcc 108
<210> 273
<211> 108
<212> DNA
<213>artificial sequence
<400> 273
gactacatgg gacataaaag catgtagatg aaaaaaaacg gtttcatcaa atagcagaac 60
agtggaccgg acgctgtaca cggtgctgga gactacgtcg taggttcc 108
<210> 274
<211> 108
<212> DNA
<213>artificial sequence
<400> 274
gactacatgg gacatctatg atggagggga tacagatgag tcggaaatgg gggattttat 60
tgataatgca catataccaa atatatatgc acatacgtcg taggttcc 108
<210> 275
<211> 108
<212> DNA
<213>artificial sequence
<400> 275
gactacatgg gacatgtaat aatgcaaaag cagcattgct agcaaaattt aaagaagtat 60
atgggttaag ttatatggaa ttagttagac cattacgtcg taggttcc 108
<210> 276
<211> 108
<212> DNA
<213>artificial sequence
<400> 276
gactacatgg gacataatga tataacagac gactgtgaaa tagcatataa atatgcatta 60
ttaggcaatg tagacagtaa tgcagctgca ttttacgtcg taggttcc 108
<210> 277
<211> 108
<212> DNA
<213>artificial sequence
<400> 277
gactacatgg gacatcaaat atttaagaaa tgcattagat ggtaatccta tatgtttaga 60
tagaaaacat aaaaatttat tacaagttaa atgtacgtcg taggttcc 108
<210> 278
<211> 108
<212> DNA
<213>artificial sequence
<400> 278
gactacatgg gacattataa tgcaattgaa ctgcaaatag cactagaaag tttaaatgaa 60
tctttgtata acacagagga atggacattg caatacgtcg taggttcc 108
<210> 279
<211> 108
<212> DNA
<213>artificial sequence
<400> 279
gactacatgg gacatacggc aaagacagtg tgacccggac cagaggcccc tggattgttt 60
gcataaccta catcccacca cagagtcctg tactacgtcg taggttcc 108
<210> 280
<211> 108
<212> DNA
<213>artificial sequence
<400> 280
gactacatgg gacattacga gacagaggta ccgggacttg gggcaggggc agggtgttta 60
caaaatggta atgaagaagg caaccaaatt gtgtacgtcg taggttcc 108
<210> 281
<211> 108
<212> DNA
<213>artificial sequence
<400> 281
gactacatgg gacatcttat acttttttat aattttatta ctgaaaaggg tgtaaccgaa 60
aacggtcatg accgaaatcg gtgcatatat atatacgtcg taggttcc 108
<210> 282
<211> 108
<212> DNA
<213>artificial sequence
<400> 282
gactacatgg gacataaata tagaggacag tgtcgccggt gcatgacgcg tgcacaagaa 60
cagcaaggca gccgcagaga aacacaagta taatacgtcg taggttcc 108
<210> 283
<211> 108
<212> DNA
<213>artificial sequence
<400> 283
gactacatgg gacatacagg tgacaccata tcagaggatg aggacgagga cgggacagat 60
acaggttcag acttggtaga ttttattgac acttacgtcg taggttcc 108
<210> 284
<211> 108
<212> DNA
<213>artificial sequence
<400> 284
gactacatgg gacataaaag taaataataa aaaagcagca atattggcta aatttaaaga 60
aacatatgga ttgtcattta cagatttggt acgtacgtcg taggttcc 108
<210> 285
<211> 108
<212> DNA
<213>artificial sequence
<400> 285
gactacatgg gacatatgac tatatagatg aaagtgacat agcatatgaa tatgcacagt 60
tagctgattg taatagtaat gcagcagcat ttttacgtcg taggttcc 108
<210> 286
<211> 108
<212> DNA
<213>artificial sequence
<400> 286
gactacatgg gacattggca atccaataag tatagataga aaacataaac atttaataca 60
aatgaagtgt ccaccaatgc taattacatc taatacgtcg taggttcc 108
<210> 287
<211> 108
<212> DNA
<213>artificial sequence
<400> 287
gactacatgg gacatatggc actagagagt cttgctaaat cagagtttaa aaatgaactg 60
tggacattgc aagatacgtg ccaagaacta tattacgtcg taggttcc 108
<210> 288
<211> 108
<212> DNA
<213>artificial sequence
<400> 288
gactacatgg gacataaaga gtgggggttc ggcgccgact agaaaaccat tcagacattg 60
tggattcaca gaaacaagtg aagtcgacgg acttacgtcg taggttcc 108
<210> 289
<211> 108
<212> DNA
<213>artificial sequence
<400> 289
gactacatgg gacatcctga aatagagata agctgtgtat attgcaaaac aaccttggaa 60
cgcacagagg tatatgaatt tgcatttaag gattacgtcg taggttcc 108
<210> 290
<211> 108
<212> DNA
<213>artificial sequence
<400> 290
gactacatgg gacatcacct aagtgactta gaggaagaat acgatgaaac agatgatgtt 60
aatcatcgac aactaccagc ccgacgagct gaatacgtcg taggttcc 108
<210> 291
<211> 108
<212> DNA
<213>artificial sequence
<400> 291
gactacatgg gacatgtatg gaacagaaag taaagaaaac agcccattag gggagcagcc 60
aggtgtggat acagatttaa gtccacgatt acatacgtcg taggttcc 108
<210> 292
<211> 108
<212> DNA
<213>artificial sequence
<400> 292
gactacatgg gacattacag ccatgtacat tatatgctca cattcaatgt ttagactgta 60
aatggggagt attaatatta gcattattgc gcttacgtcg taggttcc 108
<210> 293
<211> 108
<212> DNA
<213>artificial sequence
<400> 293
gactacatgg gacatatgtc acagtggatt aaatatagat gttcaaaaat agacgaagga 60
ggggactgga ggccaatagt acaattttta agatacgtcg taggttcc 108
<210> 294
<211> 108
<212> DNA
<213>artificial sequence
<400> 294
gactacatgg gacattccaa atgcatttcc atttgataag aatggtaatc cagtgtatga 60
aataaatgac aaaaattgga aatgtttttt tgatacgtcg taggttcc 108
<210> 295
<211> 108
<212> DNA
<213>artificial sequence
<400> 295
gactacatgg gacataacaa agacaactgt atgtcttatg tagcatggaa atgtgtgtac 60
tatatgactg aggcaggaac atgggataaa acatacgtcg taggttcc 108
<210> 296
<211> 108
<212> DNA
<213>artificial sequence
<400> 296
gactacatgg gacattgata aaaacagttt aaaatgtttg cggtatcgat ttaaaaaata 60
taatcaatac tatagttcta tatcatcaac ctgtacgtcg taggttcc 108
<210> 297
<211> 108
<212> DNA
<213>artificial sequence
<400> 297
gactacatgg gacatgtaat atagaaaatg aaaatccgca attagtaatt gcagaattaa 60
aagacctatt aaaggcaaat aataaaaaag ctgtacgtcg taggttcc 108
<210> 298
<211> 108
<212> DNA
<213>artificial sequence
<400> 298
gactacatgg gacatcacag caatgatgct aacatgtcaa tttaatgatg gagatacctg 60
gctgggtttg tggttgttat gtgcctttat tgttacgtcg taggttcc 108
<210> 299
<211> 108
<212> DNA
<213>artificial sequence
<400> 299
gactacatgg gacatctgca aaaccttcta ttactagtgg gcctatggct cgtcctcctg 60
tggtggtgga gcctgtggcc ccttcggatc cattacgtcg taggttcc 108
<210> 300
<211> 108
<212> DNA
<213>artificial sequence
<400> 300
gactacatgg gacatcaacg cttaattaca tatgataacc ctgtatatga aggggaggat 60
gttagtgtac aatttagtca tgattctata cactacgtcg taggttcc 108
<210> 301
<211> 108
<212> DNA
<213>artificial sequence
<400> 301
gactacatgg gacattatgg gaacaccctt tagtcctgta actcctgctt tacctacagg 60
ccctgttttc attacaggtt ctggatttta ttttacgtcg taggttcc 108
<210> 302
<211> 108
<212> DNA
<213>artificial sequence
<400> 302
gactacatgg gacataaata tgatgatgtt gaaaattcag ggagtggtgg taaccctgga 60
caggataaca gggttaatgt tggtatggat tattacgtcg taggttcc 108
<210> 303
<211> 108
<212> DNA
<213>artificial sequence
<400> 303
gactacatgg gacattaaca ccccaagcgg ctctttggtg tcctctgagg cacaattgtt 60
taataagcca tattggctac aaaaagccca gggtacgtcg taggttcc 108
<210> 304
<211> 108
<212> DNA
<213>artificial sequence
<400> 304
gactacatgg gacatatcag tatcctttgg gacgcaagtt tttgttacaa agtggatata 60
ggggacggtc ctctattcgt accggtgtta agctacgtcg taggttcc 108
<210> 305
<211> 108
<212> DNA
<213>artificial sequence
<400> 305
gactacatgg gacattgcaa ccgttttcgg ttgcccttgg catacacttt ccaccaattt 60
gttacaacgt gttgcctgtt aatcctatat atttacgtcg taggttcc 108
<210> 306
<211> 108
<212> DNA
<213>artificial sequence
<400> 306
gactacatgg gacatatgca ctatagagct gtacaagggg ataaacacac caaatgtaag 60
aagtgtaaca aacacaactg taatgatgat tattacgtcg taggttcc 108
<210> 307
<211> 108
<212> DNA
<213>artificial sequence
<400> 307
gactacatgg gacatcaacc acccgtggag gctaatggac atatattaat ttctgcaccc 60
actgtaacgt cacaccctat agaggaaatt ccttacgtcg taggttcc 108
<210> 308
<211> 108
<212> DNA
<213>artificial sequence
<400> 308
gactacatgg gacatgtggt gttaccagat cctaacaaat ttgcattgcc tgactcgtct 60
cttttcgatc ccacaacaca acgtttagta tggtacgtcg taggttcc 108
<210> 309
<211> 108
<212> DNA
<213>artificial sequence
<400> 309
gactacatgg gacattatgt actgttatat atatgtgtgt atgtactgtt atgtatatgt 60
gtgtatgtac tgttatatgt atgtgtgttg tattacgtcg taggttcc 108
<210> 310
<211> 108
<212> DNA
<213>artificial sequence
<400> 310
gactacatgg gacatgtgtt atacttttat gcactgtagc caactcttaa aagcattttt 60
ggcttgtagc agcacatttt tttgctctta ctgtacgtcg taggttcc 108
<210> 311
<211> 108
<212> DNA
<213>artificial sequence
<400> 311
gactacatgg gacatccgca atactactgt ggattatgtg tatatgtcac atggtgataa 60
tggagattat gtgtacatga actagagtaa acctacgtcg taggttcc 108
<210> 312
<211> 108
<212> DNA
<213>artificial sequence
<400> 312
gactacatgg gacatactat aacatcatct gaatcgacta cacctgctat tttagatgtg 60
tctgttacca atcacactac cactagtgtg ttttacgtcg taggttcc 108
<210> 313
<211> 108
<212> DNA
<213>artificial sequence
<400> 313
gactacatgg gacattagtc gcattgggca acgggggtcc atgtacacac gcagtggaca 60
acatataggt gcccgcatac attattttca ggatacgtcg taggttcc 108
<210> 314
<211> 108
<212> DNA
<213>artificial sequence
<400> 314
gactacatgg gacatgtgcc tcctcccaac cctgtatcca aggttgttgc cacggatgcg 60
tatgttaaac gcaccaacat attttatcat gcctacgtcg taggttcc 108
<210> 315
<211> 108
<212> DNA
<213>artificial sequence
<400> 315
gactacatgg gacatacaaa atggtgactg ccccccgttg gaacttatta ccagtgttat 60
acaggatggg gacatggttg atacaggctt tggtacgtcg taggttcc 108
<210> 316
<211> 108
<212> DNA
<213>artificial sequence
<400> 316
gactacatgg gacatctgtg tctaaatctg ctacatacac taattcagat tataaggaat 60
acatgcgcca tgtggaggag tttgatttac agttacgtcg taggttcc 108
<210> 317
<211> 108
<212> DNA
<213>artificial sequence
<400> 317
gactacatgg gacattattt atatgttgtt gtagtgtgta tatgtttctt gtattgtgta 60
tatgtgtata tgtttgtgta tatgtgtatg ttatacgtcg taggttcc 108
<210> 318
<211> 108
<212> DNA
<213>artificial sequence
<400> 318
gactacatgg gacatgcatt tttggcttct agctgaacat ttttgtaccc ttagtatatt 60
atgcacaata cccacaaaat gagtaaccta aggtacgtcg taggttcc 108
<210> 319
<211> 108
<212> DNA
<213>artificial sequence
<400> 319
gactacatgg gacatgccaa caacacacct ggccagggcg cggtattgca tgactaatgt 60
acaataaacc tgtcggtttg tacaatgttg tggtacgtcg taggttcc 108
<210> 320
<211> 108
<212> DNA
<213>artificial sequence
<400> 320
gactacatgg gacatgacat tatacctaag gttgaaggca aaactattgc tgaacaaata 60
ttacaatatg gaagtatggg tgtatttttt ggttacgtcg taggttcc 108
<210> 321
<211> 108
<212> DNA
<213>artificial sequence
<400> 321
gactacatgg gacattatgg atacatttat tgttagcaca aaccctaaca cagtaactag 60
tagcacaccc ataccagggt ctcgcccagt ggctacgtcg taggttcc 108
<210> 322
<211> 108
<212> DNA
<213>artificial sequence
<400> 322
gactacatgg gacatgatta tatgatattt atgcagatga ctttattaca gatacttcta 60
caaccccggt accatctgta ccctctacat ctttacgtcg taggttcc 108
<210> 323
<211> 108
<212> DNA
<213>artificial sequence
<400> 323
gactacatgg gacattaaag tatcaggatt acaatacagg gtatttagaa tacatttacc 60
tgaccccaat aagtttggtt ttcctgacac ctctacgtcg taggttcc 108
<210> 324
<211> 108
<212> DNA
<213>artificial sequence
<400> 324
gactacatgg gacataatat ccagattata ttaaaatggt gtcagaacca tatggcgaca 60
gcttattttt ttatttacga agggaacaaa tgttacgtcg taggttcc 108
<210> 325
<211> 108
<212> DNA
<213>artificial sequence
<400> 325
gactacatgg gacatactat tttggaggac tggaattttg gtctacaacc tcccccagga 60
ggcacactag aagatactta taggtttgta acctacgtcg taggttcc 108
<210> 326
<211> 108
<212> DNA
<213>artificial sequence
<400> 326
gactacatgg gacatcttat tgtttcaaca cctactaatt gtgttgtggt tattcattgt 60
atataaacta tatttgctac atcctgtttt tgttacgtcg taggttcc 108
<210> 327
<211> 108
<212> DNA
<213>artificial sequence
<400> 327
gactacatgg gacattcatt tgtaaaactg cacatgggtg tgtgcaaacc gattttgggt 60
tacacattta caagcaactt atataataat acttacgtcg taggttcc 108
<210> 328
<211> 108
<212> DNA
<213>artificial sequence
<400> 328
gactacatgg gacatcagct acagatacac ttgctcctgt aagaccccct ttaacagtag 60
atcctgtggg cccttctgat ccttctatag ttttacgtcg taggttcc 108
<210> 329
<211> 108
<212> DNA
<213>artificial sequence
<400> 329
gactacatgg gacataaact tattacatat gataatcctg catatgaagg tatagatgtg 60
gataatacat tatatttttc tagtaatgat aattacgtcg taggttcc 108
<210> 330
<211> 108
<212> DNA
<213>artificial sequence
<400> 330
gactacatgg gacattgata tacccattaa tataactgac caagctcctt cattaattcc 60
tatagttcca gggtctccac aatatacaat tattacgtcg taggttcc 108
<210> 331
<211> 108
<212> DNA
<213>artificial sequence
<400> 331
gactacatgg gacatggtgt gggcattagt ggccatcctt tattaaataa attggatgac 60
acagaaaatg ctagtgctta tgcagcaaat gcatacgtcg taggttcc 108
<210> 332
<211> 108
<212> DNA
<213>artificial sequence
<400> 332
gactacatgg gacattgggt ctactgcaaa tttagccagt tcaaattatt ttcctacacc 60
tagtggttct atggttacct ctgatgccca aattacgtcg taggttcc 108
<210> 333
<211> 108
<212> DNA
<213>artificial sequence
<400> 333
gactacatgg gacatgggaa gtaaatttaa aggaaaagtt ttctgcagac ctagatcagt 60
ttcctttagg acgcaaattt ttactacaag cagtacgtcg taggttcc 108
<210> 334
<211> 108
<212> DNA
<213>artificial sequence
<400> 334
gactacatgg gacatcacaa aatgtgtttt tttaaatagt tctatgtcag caactatggt 60
ttaaacttgt acgtttcctg cttgccatgc gtgtacgtcg taggttcc 108
<210> 335
<211> 108
<212> DNA
<213>artificial sequence
<400> 335
gactacatgg gacattgcat atacatgcta tattgtcttt acagtaattg tataggttgt 60
tttatacagt gtattgtaca ttgtatattt tgttacgtcg taggttcc 108
<210> 336
<211> 108
<212> DNA
<213>artificial sequence
<400> 336
gactacatgg gacatttgat ataacatctg cgggtacaac tacacctgcg gttttggata 60
tcacaccttc gtctacctct gtgtctattt ccatacgtcg taggttcc 108
<210> 337
<211> 108
<212> DNA
<213>artificial sequence
<400> 337
gactacatgg gacatcgctt tagtagatta ggtcaacggg caactatgtt tacccgcagc 60
ggtacacaaa taggtgctag ggttcacttt tattacgtcg taggttcc 108
<210> 338
<211> 108
<212> DNA
<213>artificial sequence
<400> 338
gactacatgg gacatggcct agtgacaata ccgtatatct tccacctcct tctgtggcaa 60
gagttgtaaa taccgatgat tatgtgactc gcatacgtcg taggttcc 108
<210> 339
<211> 108
<212> DNA
<213>artificial sequence
<400> 339
gactacatgg gacatggcac tgcttgtaaa tcgcgtcctt tatcacaggg cgattgcccc 60
cctttagaac ttaaaaacac agttttggaa gattacgtcg taggttcc 108
<210> 340
<211> 108
<212> DNA
<213>artificial sequence
<400> 340
gactacatgg gacatcagta ccaatttaac aatatgtgct tctacacagt ctcctgtacc 60
tgggcaatat gatgctacca aatttaagca gtatacgtcg taggttcc 108
<210> 341
<211> 108
<212> DNA
<213>artificial sequence
<400> 341
gactacatgg gacatccagg aagtaatatg tgtgtgtgta tatatatata catctattgt 60
tgtgtttgta tgtcctgtgt ttgtgtttgt tgttacgtcg taggttcc 108
<210> 342
<211> 108
<212> DNA
<213>artificial sequence
<400> 342
gactacatgg gacataggtg cgctacaaca attgcttgca taactatatc cactccctaa 60
gtaataaaac tgcttttagg cacatatttt agttacgtcg taggttcc 108
<210> 343
<211> 108
<212> DNA
<213>artificial sequence
<400> 343
gactacatgg gacatacaaa taggtgctag ggttcacctt tatcatgata taagtcctat 60
tgcaccttcc ccagaatata ttgaactgca gcctacgtcg taggttcc 108
<210> 344
<211> 108
<212> DNA
<213>artificial sequence
<400> 344
gactacatgg gacattatgt cctatattca tagtatgaat agcggtattt tagaggattg 60
gaactttggt gttccccccc cgccaactac tagtacgtcg taggttcc 108
<210> 345
<211> 108
<212> DNA
<213>artificial sequence
<400> 345
gactacatgg gacattactt tttttttatt attaccatgc ggtccaaacg ctctacaaaa 60
cgcactaaac gtgcgtctgc tacacaatta tattacgtcg taggttcc 108
<210> 346
<211> 108
<212> DNA
<213>artificial sequence
<400> 346
gactacatgg gacattccat ctgtattgca acctcctaca cctgcagaaa catcaggtca 60
tttactactt tcatcatctt ctattagcac acatacgtcg taggttcc 108
<210> 347
<211> 108
<212> DNA
<213>artificial sequence
<400> 347
gactacatgg gacatatcct gcaggtgaaa gtattgaaat gcaaccttta ggggcgtctg 60
caactactac ttctacttta aatgatggct tattacgtcg taggttcc 108
<210> 348
<211> 108
<212> DNA
<213>artificial sequence
<400> 348
gactacatgg gacatatatt atcacgcagg cagtgctagg ctgcttacag taggccatcc 60
atattattcc atacctaaat ctgacaatcc taatacgtcg taggttcc 108
<210> 349
<211> 108
<212> DNA
<213>artificial sequence
<400> 349
gactacatgg gacatatatg gttggtacag gctttggagc tatggatttt actgctttac 60
aagacactaa aagtaatgtt cctttggaca ttttacgtcg taggttcc 108
<210> 350
<211> 108
<212> DNA
<213>artificial sequence
<400> 350
gactacatgg gacatcatgg tgaggaattt gatttacaat ttatatttca gttatgcaaa 60
ataacattat ctgcagacat aatgacatat atttacgtcg taggttcc 108
<210> 351
<211> 108
<212> DNA
<213>artificial sequence
<400> 351
gactacatgg gacattgtat gtatgtttat gtatgcgtgt gtacttgtat atatgtatag 60
tatgttatgt gtgtatgtat gctatgtatg ttatacgtcg taggttcc 108
<210> 352
<211> 108
<212> DNA
<213>artificial sequence
<400> 352
gactacatgg gacattaaat acaactgtag ttcaactatg tgtcatgcac atatattata 60
ttgtcctaca caccttaaac tgcttttagg cactacgtcg taggttcc 108
<210> 353
<211> 108
<212> DNA
<213>artificial sequence
<400> 353
gactacatgg gacataaata ttaaggtatg gtagtatggg tgtttttttt ggtgggttgg 60
gtattgggtc cggctctggt actgggggtc gcatacgtcg taggttcc 108
<210> 354
<211> 108
<212> DNA
<213>artificial sequence
<400> 354
gactacatgg gacatattcc aggggtgcgc cgtcctgcac gtttagggtt atatagtaag 60
gctacacaac aagtaaaagt tattgatcca acgtacgtcg taggttcc 108
<210> 355
<211> 108
<212> DNA
<213>artificial sequence
<400> 355
gactacatgg gacatacagt ccacatctgc tgtgtctgcc tatgtaccta caaataccac 60
tgtgccacta agtacaggtt ttgacattcc cattacgtcg taggttcc 108
<210> 356
<211> 108
<212> DNA
<213>artificial sequence
<400> 356
gactacatgg gacattttgg atttcctgat acatcttttt ataatcctga aactcaacgc 60
ttagtttggg cctgtgttgg tttagaggta ggttacgtcg taggttcc 108
<210> 357
<211> 108
<212> DNA
<213>artificial sequence
<400> 357
gactacatgg gacattttac gtagggaaca aatgtttgta aggcattttt ttaatagatc 60
aggcacggtt ggtgaatcgg tccctactga ctttacgtcg taggttcc 108
<210> 358
<211> 108
<212> DNA
<213>artificial sequence
<400> 358
gactacatgg gacatatacc tataggtttg tcacctcaca ggccattaca tgtcaaaaaa 60
ctgcccccca aaagcccaag gaagatccat ttatacgtcg taggttcc 108
<210> 359
<211> 108
<212> DNA
<213>artificial sequence
<400> 359
gactacatgg gacatatttt ataaactatt gttcctactt gttcctactt gttcctgctc 60
ctcccaatag tcatgtactt atttctgcct atatacgtcg taggttcc 108
<210> 360
<211> 108
<212> DNA
<213>artificial sequence
<400> 360
gactacatgg gacataaggt tgtgtcatgc attataaata agttgtatgt tactcatata 60
attaattgca tataggtatt acaccgtttt cggtacgtcg taggttcc 108
<210> 361
<211> 108
<212> DNA
<213>artificial sequence
<400> 361
gactacatgg gacatgctgc aatccccttg cagcctatac gtcctccggt tactgtagac 60
actgttggac ctttagactc gcctatagtg tcatacgtcg taggttcc 108
<210> 362
<211> 108
<212> DNA
<213>artificial sequence
<400> 362
gactacatgg gacattaaac ttataacata tgataatcct gcatttgaaa gctttgaccc 60
tgaagacaca ttacaatttc aacatagtga tattacgtcg taggttcc 108
<210> 363
<211> 108
<212> DNA
<213>artificial sequence
<400> 363
gactacatgg gacattattt cccacatcta gcccatttgt tcctatttcg cctttttttc 60
cttttgacac cattgttgta gacggtgctg acttacgtcg taggttcc 108
<210> 364
<211> 108
<212> DNA
<213>artificial sequence
<400> 364
gactacatgg gacattggtc atcctttatt aaacaaattt gatgacactg aaaccggtaa 60
caagtatcct ggacaaccgg gtgctgataa tagtacgtcg taggttcc 108
<210> 365
<211> 108
<212> DNA
<213>artificial sequence
<400> 365
gactacatgg gacatttcaa agcagtgctt tttttcccac tcctagtgga tcaatggtta 60
cttccgaatc tcagttattt aataagccat atttacgtcg taggttcc 108
<210> 366
<211> 108
<212> DNA
<213>artificial sequence
<400> 366
gactacatgg gacataaatt ttcagcagat ttagatcagt ttcctttggg acgcaagttt 60
ttattacagg caggtcttaa agcaaaacct aaatacgtcg taggttcc 108
<210> 367
<211> 108
<212> DNA
<213>artificial sequence
<400> 367
gactacatgg gacatttttg acatactagt gtccatattg tacaatttcc tccattttgt 60
atgcctaacc gttttcggtt acttggcata cattacgtcg taggttcc 108
<210> 368
<211> 108
<212> DNA
<213>artificial sequence
<400> 368
gactacatgg gacatactaa taaatacctt tatattttag cagtgtatta ttatgagaca 60
caaacgatct acaaggcgca agcgtgcatc tgctacgtcg taggttcc 108
<210> 369
<211> 108
<212> DNA
<213>artificial sequence
<400> 369
gactacatgg gacattttct acacatttaa atcccacatt tactgaacca tctgtactac 60
accctccagc gcctgcagaa gcctctggac atttacgtcg taggttcc 108
<210> 370
<211> 108
<212> DNA
<213>artificial sequence
<400> 370
gactacatgg gacatataca ttattatcag gatttaagtc ctattgtgcc tttagaccgc 60
actgtgccaa atgaacaata tgaattacag ccttacgtcg taggttcc 108
<210> 371
<211> 108
<212> DNA
<213>artificial sequence
<400> 371
gactacatgg gacatatgtg tctcgcgcaa gcatttatta ttatgctggt agttccagac 60
ttcttgctgt tggccatcca tatttttcta ttatacgtcg taggttcc 108
<210> 372
<211> 108
<212> DNA
<213>artificial sequence
<400> 372
gactacatgg gacatattat tgaggatggt gatatggtgg acacaggatt tggttgcatg 60
gattttaaaa cattgcaggc taataaaagt gattacgtcg taggttcc 108
<210> 373
<211> 108
<212> DNA
<213>artificial sequence
<400> 373
gactacatgg gacatggaat atataagaca tgttgaagaa tatgatctac agtttgtttt 60
tcaactatgc aaagttacct taactgcaga cgttacgtcg taggttcc 108
<210> 374
<211> 108
<212> DNA
<213>artificial sequence
<400> 374
gactacatgg gacattttgt gtatgtgtta caatgtatgt tatgttgtat gttactgtgt 60
ttgttttaca tgtacttgtt tgtgtgcatg ttctacgtcg taggttcc 108
<210> 375
<211> 108
<212> DNA
<213>artificial sequence
<400> 375
gactacatgg gacatttttt actttacttt caaaccttat gtgcagtttt ggcttacaca 60
attgctttgt atgccaaact atgccttgta aaatacgtcg taggttcc 108
<210> 376
<211> 108
<212> DNA
<213>artificial sequence
<400> 376
gactacatgg gacatctggc acaggtggaa gatctggata tgttccactg ggtacaacac 60
ctccaacggc tgccacaaac attcctatac gactacgtcg taggttcc 108
<210> 377
<211> 108
<212> DNA
<213>artificial sequence
<400> 377
gactacatgg gacataccca gcaggttaag gttgttgacc ctgcctttat gacttctcct 60
gcaaaactta ttacatatga taatcctgca tattacgtcg taggttcc 108
<210> 378
<211> 108
<212> DNA
<213>artificial sequence
<400> 378
gactacatgg gacataccat taagtagtgg ctatgatatt cctataacag cagggccaga 60
cattgtattt aactctaata ctattactaa cactacgtcg taggttcc 108
<210> 379
<211> 108
<212> DNA
<213>artificial sequence
<400> 379
gactacatgg gacatgcctg tacaggcgtt gaagtaggtc gtggtcagcc attaggtgta 60
ggtattagtg gtcatccttt attaaataaa ttgtacgtcg taggttcc 108
<210> 380
<211> 108
<212> DNA
<213>artificial sequence
<400> 380
gactacatgg gacataggtg aaacagtacc tgcagaccta tatattaagg gtaccactgg 60
cacattgcct agtactagtt attttcctac tcctacgtcg taggttcc 108
<210> 381
<211> 108
<212> DNA
<213>artificial sequence
<400> 381
gactacatgg gacatctaaa gatgatccat taaaaaatta tactttttgg gaggttgatt 60
taaaggaaaa gttttctgca gacttagatc aattacgtcg taggttcc 108
<210> 382
<211> 108
<212> DNA
<213>artificial sequence
<400> 382
gactacatgg gacatcctcc attttgtgtg ttagtgtcat tctacctcca ttttgtgtgt 60
tagtgtcctt tacattacct ttcaaccgat ttctacgtcg taggttcc 108
<210> 383
<211> 108
<212> DNA
<213>artificial sequence
<400> 383
gactacatgg gacatattgt gtttggtact gtgtaacatg tgtgtatggt ggttttattt 60
tttgttgttc attgtatatt ttgttttttt acttacgtcg taggttcc 108
<210> 384
<211> 108
<212> DNA
<213>artificial sequence
<400> 384
gactacatgg gacatcccct gttgttacac caagggtccc acctacaaca ggttttacaa 60
taaccacatc tacagatacc acacctgcta ttttacgtcg taggttcc 108
<210> 385
<211> 108
<212> DNA
<213>artificial sequence
<400> 385
gactacatgg gacatgcact aacatctagg aaaggcacta ttagatatag tagagtaggt 60
aataaacgta ctatgcatac acgaagtgga aaatacgtcg taggttcc 108
<210> 386
<211> 108
<212> DNA
<213>artificial sequence
<400> 386
gactacatgg gacatagcta tcccatattt ttttgcagat gtctctgtgg cggtctaacg 60
aagccactgt ctacctgcct ccagtgtcag tgttacgtcg taggttcc 108
<210> 387
<211> 108
<212> DNA
<213>artificial sequence
<400> 387
gactacatgg gacattgttt aataggttgt aggcctccta taggtgaaca ttggggaaaa 60
ggcacacctt gtaatgctaa ccaggtaaaa gcatacgtcg taggttcc 108
<210> 388
<211> 108
<212> DNA
<213>artificial sequence
<400> 388
gactacatgg gacatgagta accaattgtt tgttactgta gttgatacaa cccgtagtac 60
aaatatgtct gtgtgttctg ctgtgtcttc tagtacgtcg taggttcc 108
<210> 389
<211> 108
<212> DNA
<213>artificial sequence
<400> 389
gactacatgg gacatctaaa aaatcttcta ctaaacgtag aaaagtaaaa agttaatgtg 60
taaatgtgta tgcatgtata ctgtgtgtta tgttacgtcg taggttcc 108
<210> 390
<211> 108
<212> DNA
<213>artificial sequence
<400> 390
gactacatgg gacattgatt catcttgcag tattagtcat ttttcatact tgtggtccac 60
ccacacttgt aacacttgta acagtgcttt tagtacgtcg taggttcc 108
<210> 391
<211> 108
<212> DNA
<213>artificial sequence
<400> 391
gactacatgg gacattaagc gtgcatctgc aactgaccta tatagaacct gtaaacaatc 60
gggtacctgt ccaccagacg ttgttgataa agttacgtcg taggttcc 108
<210> 392
<211> 108
<212> DNA
<213>artificial sequence
<400> 392
gactacatgg gacatcctcg ggtaatatat ttgtcagtac ccctacatca ggtacacatg 60
gctatgagga aatacctatg gaagtgtttg ccatacgtcg taggttcc 108
<210> 393
<211> 108
<212> DNA
<213>artificial sequence
<400> 393
gactacatgg gacattctga tgcttcagat gcattatttg atatatatgc tgatgtggac 60
aataacacat atttagatac tgcatttaat aattacgtcg taggttcc 108
<210> 394
<211> 108
<212> DNA
<213>artificial sequence
<400> 394
gactacatgg gacattagga catccatatt ttaaagtggg tatgaatggt ggtcgcaagc 60
aggacattcc aaaggtgtct gcatatcaat atatacgtcg taggttcc 108
<210> 395
<211> 108
<212> DNA
<213>artificial sequence
<400> 395
gactacatgg gacatttgca ggaaaccaaa agtgaggtgc ctttagatat ttgtcaatcc 60
atttgtaaat atcctgatta tttgcaaatg tcttacgtcg taggttcc 108
<210> 396
<211> 108
<212> DNA
<213>artificial sequence
<400> 396
gactacatgg gacatgtgta ctgtcacatt aacaactgat gttatgtctt atattcacac 60
tatgaattcc tctatattgg acaattggaa ttttacgtcg taggttcc 108
<210> 397
<211> 108
<212> DNA
<213>artificial sequence
<400> 397
gactacatgg gacatacagt ttcatgtgtg attgcacacc ctgtgactaa cagtgtattt 60
gttttacata taataggtct gcaacatttc atatacgtcg taggttcc 108
<210> 398
<211> 108
<212> DNA
<213>artificial sequence
<400> 398
gactacatgg gacattgctt ggttgttggt gtttcttttt atagttgtta ttacatcccc 60
attaacagca tttgctgtat acatttgttg ctatacgtcg taggttcc 108
<210> 399
<211> 108
<212> DNA
<213>artificial sequence
<400> 399
gactacatgg gacatcacct gtggttattg accctgtagg gcctactgat ccatctattg 60
ttacgttggt agaggagtcc agtgttgttt ccttacgtcg taggttcc 108
<210> 400
<211> 108
<212> DNA
<213>artificial sequence
<400> 400
gactacatgg gacattatga gcccctggac accacactat cctttgagcc taccagtaat 60
gttcctgatt cagattttat ggatattatt cgttacgtcg taggttcc 108
<210> 401
<211> 108
<212> DNA
<213>artificial sequence
<400> 401
gactacatgg gacattgctg ccacctccac ctatataggt attcatggca cacaatatta 60
tttatggcca tggtattatt attttcctaa aaatacgtcg taggttcc 108
<210> 402
<211> 108
<212> DNA
<213>artificial sequence
<400> 402
gactacatgg gacatagtgc tcatgcagct acagctgtta ttacgcagga tgttagggat 60
aatgtgtcag ttgattataa gcaaacacag ctgtacgtcg taggttcc 108
<210> 403
<211> 108
<212> DNA
<213>artificial sequence
<400> 403
gactacatgg gacatcagtg gctctattac tacttctgat tctcaattat ttaataagcc 60
atattggtta cataaggccc agggccataa caatacgtcg taggttcc 108
<210> 404
<211> 108
<212> DNA
<213>artificial sequence
<400> 404
gactacatgg gacataatat ccccttggtc gaaagttttt agttcaggct gggttacgtc 60
gtaggcctac cataggacct cgtaagcgtc ctgtacgtcg taggttcc 108
<210> 405
<211> 108
<212> DNA
<213>artificial sequence
<400> 405
gactacatgg gacatccttt taaacataat acttaaactg gcacatttac aacccctaca 60
tagtttaaac tactggcgcg ccttcttggc gtatacgtcg taggttcc 108
<210> 406
<211> 108
<212> DNA
<213>artificial sequence
<400> 406
gactacatgg gacatcaact gacttatata gaacatgtaa gcaatccggt acgtgccccc 60
ctgatgttat taacaaggtg gaaggcacaa ccttacgtcg taggttcc 108
<210> 407
<211> 108
<212> DNA
<213>artificial sequence
<400> 407
gactacatgg gacataatca acaggtccgt gtgtccacct cacagttttt aacacatccc 60
tcatcgttgg ttacatttga taatccagct tattacgtcg taggttcc 108
<210> 408
<211> 108
<212> DNA
<213>artificial sequence
<400> 408
gactacatgg gacatatatt ttagggttgt acctaatggt gcaggtaata aacaggctgt 60
tcctaaggta tccgcatatc agtatagggt gtttacgtcg taggttcc 108
<210> 409
<211> 108
<212> DNA
<213>artificial sequence
<400> 409
gactacatgg gacatagttc aggctgggtt acgtcgtagg cctaccatag gacctcgtaa 60
gcgtcctgct gcttccacgt ctactgcatc tactacgtcg taggttcc 108
<210> 410
<211> 108
<212> DNA
<213>artificial sequence
<400> 410
gactacatgg gacatcctaa actggcacat ttacaacccc tacatagttt aacctactgg 60
cgcgccttct tggcgtacat gtggcacacc tggtacgtcg taggttcc 108
<210> 411
<211> 108
<212> DNA
<213>artificial sequence
<400> 411
gactacatgg gacattttgt ctaataaata cttttatatt ttttaatagc ttgtcgcaat 60
gagatacaga cggtctacac ggcacaaacg tgctacgtcg taggttcc 108
<210> 412
<211> 108
<212> DNA
<213>artificial sequence
<400> 412
gactacatgg gacataatca gtttctacac atttaaatcc tacattcact gaaccatcta 60
taatacagcc cccggcacct gcagaagcat ctgtacgtcg taggttcc 108
<210> 413
<211> 108
<212> DNA
<213>artificial sequence
<400> 413
gactacatgg gacatgcacg ggtacattat tatcatgata ttagtcctat acagcctgct 60
gaagttcagg aagacataga agtgcaacct ttatacgtcg taggttcc 108
<210> 414
<211> 108
<212> DNA
<213>artificial sequence
<400> 414
gactacatgg gacatagtat gtgtctcgca caagcatcta ttattatgca ggcagttcta 60
gattacttac agtaggacat ccctattttt ctatacgtcg taggttcc 108
<210> 415
<211> 108
<212> DNA
<213>artificial sequence
<400> 415
gactacatgg gacatctcat taacagtgta atacaggatg gggacatggt agatacagga 60
tttggttgca tggattttaa taccttgcaa gcttacgtcg taggttcc 108
<210> 416
<211> 108
<212> DNA
<213>artificial sequence
<400> 416
gactacatgg gacattaaaa atgaaaattt taaggaatac cttcgtcatg gcgaggaatt 60
tgatttacaa tttatttttc aattgtgcaa aattacgtcg taggttcc 108
<210> 417
<211> 108
<212> DNA
<213>artificial sequence
<400> 417
gactacatgg gacatggtaa ccattgtttg ttatgtaatt gttttctgtg tgtactgtgt 60
tgtttgcatg ttatgtatgt gtgtgtgcat gtttacgtcg taggttcc 108
<210> 418
<211> 108
<212> DNA
<213>artificial sequence
<400> 418
gactacatgg gacatataca ttgcctaaca ttgcatgttt taaactgctt ttaggcacat 60
attttattta aactttcaat gcactaatta cagtacgtcg taggttcc 108
<210> 419
<211> 108
<212> DNA
<213>artificial sequence
<400> 419
gactacatgg gacatgcaaa gcctctggca cctgcccccc cgatgttatt cctaaagtgg 60
aaggcacaac tattgcagat caacttttaa aattacgtcg taggttcc 108
<210> 420
<211> 108
<212> DNA
<213>artificial sequence
<400> 420
gactacatgg gacatattag tacacacacc tatgaagaaa tccctatgga tacatttgtt 60
acctctactg acagcagcag tgtaacaagt agttacgtcg taggttcc 108
<210> 421
<211> 108
<212> DNA
<213>artificial sequence
<400> 421
gactacatgg gacattatta atgatggttt gtatgatgtg tatgcagagt ctttgcagca 60
acccacgttt cacttacctt ccacactttc tactacgtcg taggttcc 108
<210> 422
<211> 108
<212> DNA
<213>artificial sequence
<400> 422
gactacatgg gacataaaaa agttttagtt cccaaggtgt ctggcctgca atacagggta 60
tttagaatta aattgccgga ccctaataaa ttttacgtcg taggttcc 108
<210> 423
<211> 108
<212> DNA
<213>artificial sequence
<400> 423
gactacatgg gacatatgta gcagtgtatg taagtatcca gattatttgc aaatggctag 60
cgagccatat ggtgacagtt tgttcttttt tcttacgtcg taggttcc 108
<210> 424
<211> 108
<212> DNA
<213>artificial sequence
<400> 424
gactacatgg gacatatatt cataagatgg atgccactat tttagaggac tggcaatttg 60
gccttacccc accaccgtct gcatctttgg aggtacgtcg taggttcc 108
<210> 425
<211> 108
<212> DNA
<213>artificial sequence
<400> 425
gactacatgg gacattttgt gtatgtatta ataaagtact gtatttacta aactatttat 60
attagtctta tgttatggtt gcacccacat gagtacgtcg taggttcc 108
<210> 426
<211> 108
<212> DNA
<213>artificial sequence
<400> 426
gactacatgg gacatcctac gccaaatatg tcttgtaaaa cataattgaa tactgttact 60
caccaggtgt gcactacacg accggttacg gtttacgtcg taggttcc 108
<210> 427
<211> 108
<212> DNA
<213>artificial sequence
<400> 427
gactacatgg gacataggcc ttccacaata gttgatgtaa ctccggcgcg accacctatt 60
gttgtggaat ccgtagggcc tacagaccct tcctacgtcg taggttcc 108
<210> 428
<211> 108
<212> DNA
<213>artificial sequence
<400> 428
gactacatgg gacattagac ctgcaacatt agtatctgct gataatccac tttttgaagg 60
tactgacaca tctttagctt tttctccgtc gggtacgtcg taggttcc 108
<210> 429
<211> 108
<212> DNA
<213>artificial sequence
<400> 429
gactacatgg gacatctaca ggccccagta cgtggccctt tgttcctcag tctccttatg 60
atgttaccca tgatgtatat atacagggat ccttacgtcg taggttcc 108
<210> 430
<211> 108
<212> DNA
<213>artificial sequence
<400> 430
gactacatgg gacatccatc cattgtttaa taggctggat gatactgaaa gttccaattt 60
agcaaataat aatgttatag aagatagtag ggatacgtcg taggttcc 108
<210> 431
<211> 108
<212> DNA
<213>artificial sequence
<400> 431
gactacatgg gacatctccg agttctgtat atgttgctac gcctagtggg tctatgatta 60
cgtctgaggc acagttattt aataaacctt atttacgtcg taggttcc 108
<210> 432
<211> 108
<212> DNA
<213>artificial sequence
<400> 432
gactacatgg gacatagttt ttctacagac ctggatcaat ttccactggg tagaaaattt 60
ttaatgcaac tgggcactag gtcaaagcct gcttacgtcg taggttcc 108
<210> 433
<211> 108
<212> DNA
<213>artificial sequence
<400> 433
gactacatgg gacataattc ggttgcatgg cctagtgcca ttatttaaac taaaaggaat 60
tcggttgcat ggcctagtgc cattatttaa acctacgtcg taggttcc 108
<210> 434
<211> 108
<212> DNA
<213>artificial sequence
<400> 434
gactacatgg gacatacata tttcggaggc cttggcattg gtacaggaac tgggtctggg 60
ggtcgtgcag gctatgttcc attggggtct aggtacgtcg taggttcc 108
<210> 435
<211> 108
<212> DNA
<213>artificial sequence
<400> 435
gactacatgg gacattattc gggtcctgac atagtgttgc ctacaggccc cagtacgtgg 60
cccttcgttc ctcagtctcc ttatgatgtt acctacgtcg taggttcc 108
<210> 436
<211> 108
<212> DNA
<213>artificial sequence
<400> 436
gactacatgg gacatttatg gcatatttac ataatatgaa tgctaaccta ctggaggact 60
ggaatattgg gttatccccg ccagtggcca ctatacgtcg taggttcc 108
<210> 437
<211> 108
<212> DNA
<213>artificial sequence
<400> 437
gactacatgg gacatataaa ctaaggtgtg tcattattgt ggcttttgtt ttgtaagtta 60
ttgtgtacag tgcactatgt gtattgtgca tactacgtcg taggttcc 108
<210> 438
<211> 108
<212> DNA
<213>artificial sequence
<400> 438
gactacatgg gacattgatg tgtattaatt ttcatgcaca atacttaacc caacaagact 60
gaatgtatac tggtgttgca catggtggta tggtacgtcg taggttcc 108
<210> 439
<211> 108
<212> DNA
<213>artificial sequence
<400> 439
gactacatgg gacatctagt tttatagacg ccggtgcacc agccccatca attcccactc 60
catctggctt tgatattacc acctctgcag atatacgtcg taggttcc 108
<210> 440
<211> 108
<212> DNA
<213>artificial sequence
<400> 440
gactacatgg gacatgatcc tgattttcta gatattgttg cattgcacag acctgcatta 60
acctctcgca ggggtactgt acgttatagt aggtacgtcg taggttcc 108
<210> 441
<211> 108
<212> DNA
<213>artificial sequence
<400> 441
gactacatgg gacattttta tgttgcaccc tagttatttt attttgcgtc gcagacgtaa 60
acgttttcca tatttttttg cagatgtccg tgttacgtcg taggttcc 108
<210> 442
<211> 108
<212> DNA
<213>artificial sequence
<400> 442
gactacatgg gacataggga atgcttatct atggattata aacaaacaca attatgttta 60
attggctgta aacctcccac tggtgagcat tggtacgtcg taggttcc 108
<210> 443
<211> 108
<212> DNA
<213>artificial sequence
<400> 443
gactacatgg gacatttggc tacagcgtgc acaaggtcat aacaatggca tttgctgggg 60
caatcagtta tttgttaccg tggttgatac cactacgtcg taggttcc 108
<210> 444
<211> 108
<212> DNA
<213>artificial sequence
<400> 444
gactacatgg gacatgacta aaacgttcag cccctactac ccgtgcacca tccaccaaac 60
gcaaaaaggt taaaaaataa ttgttgtggt acttacgtcg taggttcc 108
<210> 445
<211> 108
<212> DNA
<213>artificial sequence
<400> 445
gactacatgg gacattactt gcatatgtga ctcatatata catgcagtgc agttgcaaaa 60
tgtttaatta tactcatagt ttaaacatgc ttatacgtcg taggttcc 108
<210> 446
<211> 108
<212> DNA
<213>artificial sequence
<400> 446
gactacatgg gacatcacct gatgttatac ccaaagttga aggcactact atagcagatc 60
aaatattacg atatggtagc ttaggggtgt ttttacgtcg taggttcc 108
<210> 447
<211> 108
<212> DNA
<213>artificial sequence
<400> 447
gactacatgg gacataacat accaatggat acctttgtta tttctactga cagtggcaat 60
gtcacgtcta gcacacccat tccagggtct cgctacgtcg taggttcc 108
<210> 448
<211> 108
<212> DNA
<213>artificial sequence
<400> 448
gactacatgg gacatacttt atgatattta tgctgacgat gctgatacta tacatgattt 60
tcagagtcct ctgcactcac atacgtcctt tgctacgtcg taggttcc 108
<210> 449
<211> 108
<212> DNA
<213>artificial sequence
<400> 449
gactacatgg gacatttagt tcccaaggta tcaggcttac agtatagggt ctttagggtg 60
cgtttacctg atcccaataa atttggtttt ccttacgtcg taggttcc 108
<210> 450
<211> 108
<212> DNA
<213>artificial sequence
<400> 450
gactacatgg gacatatgca aatatccaga ttatttaaaa atggccagtg aaccttatgg 60
ggatagtttg ttcttttttc ttagacgtga gcatacgtcg taggttcc 108
<210> 451
<211> 108
<212> DNA
<213>artificial sequence
<400> 451
gactacatgg gacatccaat attttggagg actggcaatt tggtttaaca cctcctccgt 60
ctgccagttt acaggacaca tatagatttg ttatacgtcg taggttcc 108
<210> 452
<211> 108
<212> DNA
<213>artificial sequence
<400> 452
gactacatgg gacattgtat tgtttgggtg tatctatgag taaggtgctg tccctaaatt 60
gccctaccct gccctgccta ttatgcatac ctatacgtcg taggttcc 108
<210> 453
<211> 108
<212> DNA
<213>artificial sequence
<400> 453
gactacatgg gacattttat aattccactg ttactactat atgcccatgc aatactgtcc 60
atacaataat tgttgtatat tgtaaattac atttacgtcg taggttcc 108
<210> 454
<211> 108
<212> DNA
<213>artificial sequence
<400> 454
gactacatgg gacatggagc ccctgcccca acatttacag gtacttcagg atttgaaata 60
tctacctcta gtacaacaac accagctgtt ttgtacgtcg taggttcc 108
<210> 455
<211> 108
<212> DNA
<213>artificial sequence
<400> 455
gactacatgg gacattaggc ctgcattaac atccagacgc agcactgtaa ggtttagtag 60
gctaggacaa cgggcaacca tgtttacccg tagtacgtcg taggttcc 108
<210> 456
<211> 108
<212> DNA
<213>artificial sequence
<400> 456
gactacatgg gacataacgt aaacgtgttc cctatttttt tacagatggc tctatggcgt 60
tctagtgaca acaaggtgta tctacctcca ccttacgtcg taggttcc 108
<210> 457
<211> 108
<212> DNA
<213>artificial sequence
<400> 457
gactacatgg gacattatta ttggctgtgt acctgccatt ggagaacact ggacaaaggg 60
cactgcttgt aagcctacta ctgtggttca gggtacgtcg taggttcc 108
<210> 458
<211> 108
<212> DNA
<213>artificial sequence
<400> 458
gactacatgg gacatgttgg cacaatcaat tgtttttaac agttgtagat actactcgca 60
gcaccaatct ttctgtgtgt gcttctacta ctttacgtcg taggttcc 108
<210> 459
<211> 108
<212> DNA
<213>artificial sequence
<400> 459
gactacatgg gacatcccac ctctacccca tcaccaaaac gtgttaagcg tcgcaagtct 60
tccagaaaat agtgttgttt gctatgtgtt tgttacgtcg taggttcc 108
<210> 460
<211> 108
<212> DNA
<213>artificial sequence
<400> 460
gactacatgg gacatctaac acaacttacg aacgccaaat agttagtcat catcctgtcc 60
aggtgcactc taacaatacc ttgcataact ttgtacgtcg taggttcc 108
<210> 461
<211> 108
<212> DNA
<213>artificial sequence
<400> 461
gactacatgg gacatttcaa attagtagct ctagttttat aaatcctgca tttacagacc 60
cttctgtcat tgaggttccc caaacaggtg aaatacgtcg taggttcc 108
<210> 462
<211> 108
<212> DNA
<213>artificial sequence
<400> 462
gactacatgg gacatcccac gcaaacgtgc agcgcctgcc cctacctcta ccccatcacc 60
aaaacgtgtt aagcgtcgca agtcttccag aaatacgtcg taggttcc 108
<210> 463
<211> 108
<212> DNA
<213>artificial sequence
<400> 463
gactacatgg gacataattt gtgtgtttgc gctttgcttt tgtgtttgtc tgtgtgtgtg 60
ccattttgtc ccgcttttgc tatctgcatc ttttacgtcg taggttcc 108
<210> 464
<211> 108
<212> DNA
<213>artificial sequence
<400> 464
gactacatgg gacattcctg cacgaccacc tattgtggtg gagtcagttg ggcctacaga 60
tccttctatt gttacactgg tagaagaatc tagtacgtcg taggttcc 108
<210> 465
<211> 108
<212> DNA
<213>artificial sequence
<400> 465
gactacatgg gacatataat cctgtttttg aaggtgctga cacaacgttg accttttctc 60
cctcgggtgt ggctcctgat cctgatttta tggtacgtcg taggttcc 108
<210> 466
<211> 108
<212> DNA
<213>artificial sequence
<400> 466
gactacatgg gacatgtacc tcagtctcct tctgatgtta cacatgatgt atatatacag 60
ggagctacat ttgcactatg gcctgtatat ttttacgtcg taggttcc 108
<210> 467
<211> 108
<212> DNA
<213>artificial sequence
<400> 467
gactacatgg gacatacact gaggtctcta atttagcagg taataatgtt atagaagata 60
gccgggacaa tatatctgtt gattgtaaac aaatacgtcg taggttcc 108
<210> 468
<211> 108
<212> DNA
<213>artificial sequence
<400> 468
gactacatgg gacatcctag tgggtccatg attacctctg aggcccaatt atttaataaa 60
ccttattggt tgcaacgtgc acagggccat aattacgtcg taggttcc 108
<210> 469
<211> 108
<212> DNA
<213>artificial sequence
<400> 469
gactacatgg gacattcctt tgggtagaaa atttttaatg caactaggcc ctagaccccc 60
tagacccaag gctagtgtat ctgcctctaa aagtacgtcg taggttcc 108
<210> 470
<211> 108
<212> DNA
<213>artificial sequence
<400> 470
gactacatgg gacatcgact tttcagcaaa acagttaatc ctttggcata ttgccgtttc 60
ctgttgtatg attcaggtat gtacactgcc ttatacgtcg taggttcc 108
<210> 471
<211> 108
<212> DNA
<213>artificial sequence
<400> 471
gactacatgg gacataacgc gcatctgcca cacaattata taaaacctgc aaattatctg 60
gtacatgtcc tgaggatgtt attaataagg tagtacgtcg taggttcc 108
<210> 472
<211> 108
<212> DNA
<213>artificial sequence
<400> 472
gactacatgg gacattctgg taatattttg attagcactc ctacatctgg aatacatagc 60
tatgaggaaa tacctatgca aacatttgct atatacgtcg taggttcc 108
<210> 473
<211> 108
<212> DNA
<213>artificial sequence
<400> 473
gactacatgg gacatatttg atggcctata tgatatttat gcaaatattg atgatgaggc 60
acccatttca tttcgtcagt ctggtgctac acctacgtcg taggttcc 108
<210> 474
<211> 108
<212> DNA
<213>artificial sequence
<400> 474
gactacatgg gacatgtttc taaatctggt accaaaacaa acatccctaa agttagtgca 60
tatcagtata gagtgtttag ggtacggttg ccttacgtcg taggttcc 108
<210> 475
<211> 108
<212> DNA
<213>artificial sequence
<400> 475
gactacatgg gacatggtgc cattggacat tgtacaatct acatgtaaat atcctgatta 60
tctaaaaatg tctgcagatg cctatgggga ttctacgtcg taggttcc 108
<210> 476
<211> 108
<212> DNA
<213>artificial sequence
<400> 476
gactacatgg gacatttatg gcatatttgc ataatatgaa taatacttta ttagacgatt 60
ggaacattgg attgtcccca ccagttgcaa ctatacgtcg taggttcc 108
<210> 477
<211> 108
<212> DNA
<213>artificial sequence
<400> 477
gactacatgg gacatgttac ttttacgcgt ggttgcataa actaaggtgc ggtagtatcc 60
ttgggcagtg tgtgtcaggt taggtggtgt tcctacgtcg taggttcc 108
<210> 478
<211> 108
<212> DNA
<213>artificial sequence
<400> 478
gactacatgg gacatgctcg ttatagtatg ccttaagttt tgtattgtgc atttgtattg 60
gtgtatattt gtataaataa atatggtatc acatacgtcg taggttcc 108
<210> 479
<211> 108
<212> DNA
<213>artificial sequence
<400> 479
gactacatgg gacatcttcg tctgggtctg tgcaagtaag cagtactagt tttactaacc 60
ctgcatttgc agaccccact attatagaag tgctacgtcg taggttcc 108
<210> 480
<211> 108
<212> DNA
<213>artificial sequence
<400> 480
gactacatgg gacatcaaat cggggcacag gtgcactatt atcatgatat tagtggcatt 60
gctcctgctg acagcattga actacaacct ttgtacgtcg taggttcc 108
<210> 481
<211> 108
<212> DNA
<213>artificial sequence
<400> 481
gactacatgg gacatccccc tcagtggcga aggttgtcaa tacagatgat tacgtaacac 60
gcactggcat ttattactat gctggtacat ctatacgtcg taggttcc 108
<210> 482
<211> 108
<212> DNA
<213>artificial sequence
<400> 482
gactacatgg gacatcccgg ggactgtcca ccattggaat tagtaaatac acctattcag 60
gatggcgata tgattgatac aggatatggt gcttacgtcg taggttcc 108
<210> 483
<211> 108
<212> DNA
<213>artificial sequence
<400> 483
gactacatgg gacattgaat cagctgtacc aaatatttat gatcctaata aatttaagga 60
atatattagg catgttgagg aatatgattt gcatacgtcg taggttcc 108
<210> 484
<211> 108
<212> DNA
<213>artificial sequence
<400> 484
gactacatgg gacattggtt gtatgtgtgg ttgtatatgt gtcatgttgt tgttggtatg 60
ttgtgcatgt atgtgtatat gtatatgtgt atgtacgtcg taggttcc 108
<210> 485
<211> 108
<212> DNA
<213>artificial sequence
<400> 485
gactacatgg gacattaata aaactgcttt taggcatagg tttttaactg tttttacttg 60
cctaatagca tagttggcct gtataactac ttttacgtcg taggttcc 108
<210> 486
<211> 108
<212> DNA
<213>artificial sequence
<400> 486
gactacatgg gacatgtact ggctctggca ccggaggtcg tgctgggtac attcctttag 60
gtggaaaacc taatactgtt gtggatgtgt cgctacgtcg taggttcc 108
<210> 487
<211> 108
<212> DNA
<213>artificial sequence
<400> 487
gactacatgg gacattatag tagggcacat caacaggttc gtgttagtaa ttttgatttt 60
gtaacgcacc cttcatcatt tgtaacattt gattacgtcg taggttcc 108
<210> 488
<211> 108
<212> DNA
<213>artificial sequence
<400> 488
gactacatgg gacattacta ccattcctct tggtactgct tggaacacgc ctgtaaatac 60
tggtcctgat gttgtattac catctacaac ccctacgtcg taggttcc 108
<210> 489
<211> 108
<212> DNA
<213>artificial sequence
<400> 489
gactacatgg gacatcgcat ggtatgggcc tgtgttggtg ttgaaatagg tagggggcag 60
ccattgggcg ttggccttag tgggcatcca ctatacgtcg taggttcc 108
<210> 490
<211> 108
<212> DNA
<213>artificial sequence
<400> 490
gactacatgg gacatgggag gcatggtagg ggacactatt cccactgaca tgtatattaa 60
gggcactgac attcgtgaaa ctcctagtag ttatacgtcg taggttcc 108
<210> 491
<211> 108
<212> DNA
<213>artificial sequence
<400> 491
gactacatgg gacataaaag gacgcccctg cacctgttaa aaaagatccc tatgatggtc 60
ttaacttttg gaatgtggat ttaaaggaaa agttacgtcg taggttcc 108
<210> 492
<211> 108
<212> DNA
<213>artificial sequence
<400> 492
gactacatgg gacatatatg caatatattt atggttgcaa actataccgt gtggccattt 60
tgtatggccg ggttgtgtgc gaccgctttc ggttacgtcg taggttcc 108
<210> 493
<211> 108
<212> DNA
<213>artificial sequence
<400> 493
gactacatgg gacattggtt tgtggtttca acatcattta cagctacgtt ttttgtatat 60
atttgtttgt tttatatacc tgcatttttg ttatacgtcg taggttcc 108
<210> 494
<211> 108
<212> DNA
<213>artificial sequence
<400> 494
gactacatgg gacatagtgg tggggatggg tttgaggtca ctacctcctc tactacaacc 60
cctgctgtgt tagatattac cccctcccct ggatacgtcg taggttcc 108
<210> 495
<211> 108
<212> DNA
<213>artificial sequence
<400> 495
gactacatgg gacatttctc gcagaggcac tgtaaggttt agcaggttag gtcaaaaggc 60
cacacttaaa acacgtagtg gtaaacaaat tggtacgtcg taggttcc 108
<210> 496
<211> 108
<212> DNA
<213>artificial sequence
<400> 496
gactacatgg gacatcttgt tttttttcag atggccttgc ggcgtactag tgacagcaag 60
gtatatctgc ctcctacccc tgtgtcccgg gtttacgtcg taggttcc 108
<210> 497
<211> 108
<212> DNA
<213>artificial sequence
<400> 497
gactacatgg gacattttag gtgaacactg gggcgttggc actgtatgta aaaatgcaca 60
gtctcaggta cagcgtggcg attgtccacc ttttacgtcg taggttcc 108
<210> 498
<211> 108
<212> DNA
<213>artificial sequence
<400> 498
gactacatgg gacataattg tttgttactt gtgtagatac tacccgcagt accaacctca 60
ctattagtac tgtatctgca caatctgcat ctgtacgtcg taggttcc 108
<210> 499
<211> 108
<212> DNA
<213>artificial sequence
<400> 499
gactacatgg gacattcctc taccacacgt aaaaaacgca aacttactaa gtaattccac 60
atggttgtgt cagtgtatag ttttgtatgt gtgtacgtcg taggttcc 108
<210> 500
<211> 108
<212> DNA
<213>artificial sequence
<400> 500
gactacatgg gacattttac taagtgcata gttggctagc agttttactg ttttatgcca 60
actatatgtc tgacacattc cttttaacaa taatacgtcg taggttcc 108
<210> 501
<211> 108
<212> DNA
<213>artificial sequence
<400> 501
gactacatgg gacatagtat tattaattca ggatctacta tacctacatt tactggtact 60
gatggctttg aaataacttc ctcttctaca acatacgtcg taggttcc 108
<210> 502
<211> 108
<212> DNA
<213>artificial sequence
<400> 502
gactacatgg gacatttttc tagatattat taaactgcac cgccctgcgc ttacatctcg 60
cagaggtaca gtgcgcttta gtagactggg tcatacgtcg taggttcc 108
<210> 503
<211> 108
<212> DNA
<213>artificial sequence
<400> 503
gactacatgg gacatattac tatttgtggc cctatacata tttattacgc aaacgccgta 60
aacgtatacc ctattttttt gcagatggct ttgtacgtcg taggttcc 108
<210> 504
<211> 108
<212> DNA
<213>artificial sequence
<400> 504
gactacatgg gacattaaca tatctgtaga caacaaacaa acacagttat gcattatagg 60
ctgtgctcct cctattgggg aacactgggc cactacgtcg taggttcc 108
<210> 505
<211> 108
<212> DNA
<213>artificial sequence
<400> 505
gactacatgg gacatattgg ttgcatcgcg cccagggcca caataatggc atttgctgga 60
ataatcagct ttttattact tgtgttgaca ctatacgtcg taggttcc 108
<210> 506
<211> 108
<212> DNA
<213>artificial sequence
<400> 506
gactacatgg gacataaggc caggccaggc cttaaaaggc ctgccccatc ctcttcctct 60
tcctcttcag ccaaacgtaa acgggttaaa aaatacgtcg taggttcc 108
<210> 507
<211> 108
<212> DNA
<213>artificial sequence
<400> 507
gactacatgg gacatggttg ctaacatttc actacatttg ccaactttta aaccacagta 60
cctatgttta ttggcataaa ccttaattct ttttacgtcg taggttcc 108
<210> 508
<211> 108
<212> DNA
<213>artificial sequence
<400> 508
gactacatgg gacatctaca tttactgtat acttgatttg tttttatatt ccatgctttt 60
tattacattt atataccttt tctattttgc aactacgtcg taggttcc 108
<210> 509
<211> 108
<212> DNA
<213>artificial sequence
<400> 509
gactacatgg gacatctttg aaattacttc ctcttctaca acaactcctg cggttttaga 60
cattacacct tccgctggta cagtacatgt tactacgtcg taggttcc 108
<210> 510
<211> 108
<212> DNA
<213>artificial sequence
<400> 510
gactacatgg gacatgtaca gtgcggttta gtagattggg tcaaaaggcc accatacgca 60
cacgtagtgg caaacaaata ggtgctcgtg gactacgtcg taggttcc 108
<210> 511
<211> 108
<212> DNA
<213>artificial sequence
<400> 511
gactacatgg gacattatac cctatttttt tgcagatggc tttgtggcgt actaatgaca 60
gcaaagtgta tttaccacct gcaccagtgt cactacgtcg taggttcc 108
<210> 512
<211> 108
<212> DNA
<213>artificial sequence
<400> 512
gactacatgg gacatgctcc tcctattggg gaacactggg ccacaggtac cacatgtaaa 60
aacgtacctg tacctcaggg tgactgtcca ccttacgtcg taggttcc 108
<210> 513
<211> 108
<212> DNA
<213>artificial sequence
<400> 513
gactacatgg gacattcagc tttttattac ttgtgttgac actactaaaa gtaccaattt 60
aaccattagc actgctgtta ctccatctgt tgctacgtcg taggttcc 108
<210> 514
<211> 108
<212> DNA
<213>artificial sequence
<400> 514
gactacatgg gacatcgtct gccaaacgta aaagggttaa aaaataggtt cgccctgcct 60
atgtatgtgt ttgtgtgtgt gtgtgtgtgt gtgtacgtcg taggttcc 108
<210> 515
<211> 108
<212> DNA
<213>artificial sequence
<400> 515
gactacatgg gacatggtat gtacatcccg cccgcccacg ccttgttcct ggcgcgcctt 60
cctggcgcat gtcatacaca ccacattact cattacgtcg taggttcc 108
<210> 516
<211> 108
<212> DNA
<213>artificial sequence
<400> 516
gactacatgg gacatttggc cgataaaata ttacagtgga gtgggttggg tatatttttg 60
ggtggcctag gtattggtac tgggtctgga tcttacgtcg taggttcc 108
<210> 517
<211> 108
<212> DNA
<213>artificial sequence
<400> 517
gactacatgg gacattactg gtactgaacc tattagcagc acacctactc caggggttag 60
tcgcatagct gctccccgct tgtatagtaa gtctacgtcg taggttcc 108
<210> 518
<211> 108
<212> DNA
<213>artificial sequence
<400> 518
gactacatgg gacatactcc tctttgtcta ggcagttgcc ctccttatct tcatctatgt 60
cttcatctta tgcaaatgtt actattccat ttttacgtcg taggttcc 108
<210> 519
<211> 108
<212> DNA
<213>artificial sequence
<400> 519
gactacatgg gacataccag atcctaacaa gtttggactc ccggatccaa atttatataa 60
tccagacaca gataggttgg tgtggggttg tgttacgtcg taggttcc 108
<210> 520
<211> 108
<212> DNA
<213>artificial sequence
<400> 520
gactacatgg gacatattcc atgttttttc atttacgcag ggagcaaatc tttgctaggc 60
actattataa taaacttgta agtgttgggg aagtacgtcg taggttcc 108
<210> 521
<211> 108
<212> DNA
<213>artificial sequence
<400> 521
gactacatgg gacatccgtc tgctagtttg gaggatgcat ataggtttgt tagaaatgca 60
gctactagct gtcaaaagga cacccctcca cagtacgtcg taggttcc 108
<210> 522
<211> 108
<212> DNA
<213>artificial sequence
<400> 522
gactacatgg gacatattat ccccgtaggt caagggtggt gtttcggtgg cgtccctatt 60
gccctaccca ttttttgcag cacaacagtt tattacgtcg taggttcc 108
<210> 523
<211> 108
<212> DNA
<213>artificial sequence
<400> 523
gactacatgg gacatcatcc caattaacta cattttttgt atatttgatt tttttttact 60
taccttgttt acttttacat ctatatacat ttttacgtcg taggttcc 108
<210> 524
<211> 108
<212> DNA
<213>artificial sequence
<400> 524
gactacatgg gacatctttg aaattacttc atcttccaca acaacccctg ctgtgttgga 60
catcacccca tctgctggta ctgtacatgt ttctacgtcg taggttcc 108
<210> 525
<211> 108
<212> DNA
<213>artificial sequence
<400> 525
gactacatgg gacatgcaca gtacgcttta gtaggttagg tcaaaaggcc accatgcgca 60
ctcgtagtgg caaacaaatt ggtgctcgtg tactacgtcg taggttcc 108
<210> 526
<211> 108
<212> DNA
<213>artificial sequence
<400> 526
gactacatgg gacattttac agatggcatt gtggcgcact aatgacagca aggtgtattt 60
gccacctgca cctgtgtctc gaagtgtgaa tactacgtcg taggttcc 108
<210> 527
<211> 108
<212> DNA
<213>artificial sequence
<400> 527
gactacatgg gacatgggaa cactggggta ttggcactac atgcaaaaac acacctgtac 60
ctccaggaga ctgccccccc ctggaacttg tattacgtcg taggttcc 108
<210> 528
<211> 108
<212> DNA
<213>artificial sequence
<400> 528
gactacatgg gacatacctg tgttgatact accagaagta caaatttaac tattagcact 60
gccactgctg cggtttcccc aacatttact ccatacgtcg taggttcc 108
<210> 529
<211> 108
<212> DNA
<213>artificial sequence
<400> 529
gactacatgg gacatacgta aacgtgttaa aaagtaatgt atgttagttt ttgtatgctt 60
gtgcacactg ttgtatgcct gtatgtatat gtttacgtcg taggttcc 108
<210> 530
<211> 108
<212> DNA
<213>artificial sequence
<400> 530
gactacatgg gacatgtact tggcgcgcct taccggcgct agtcatacaa cctattagtc 60
atttgtactt taacaattgt tggcacactg ttttacgtcg taggttcc 108
<210> 531
<211> 108
<212> DNA
<213>artificial sequence
<400> 531
gactacatgg gacatgatgt tattcctaaa attgaaggtt ctacccttgc tgataagata 60
ttacaatgga gtggtttggg aatattttta ggttacgtcg taggttcc 108
<210> 532
<211> 108
<212> DNA
<213>artificial sequence
<400> 532
gactacatgg gacataatgg aagtatttgc ttctactaat ggaacaggat tagaacctat 60
tagtagtaca cctattcctg gtatacaacg agttacgtcg taggttcc 108
<210> 533
<211> 108
<212> DNA
<213>artificial sequence
<400> 533
gactacatgg gacatctagc atacatacgc cgcgcatgtc atattcccct acaacattac 60
cagttccaag atatgcctcc aatgtgtttt ccttacgtcg taggttcc 108
<210> 534
<211> 108
<212> DNA
<213>artificial sequence
<400> 534
gactacatgg gacatggtat ctgcctatca gtacagggta tttagagtgc acctacctga 60
tcctaataaa tttggattgc ctgatccaca gtttacgtcg taggttcc 108
<210> 535
<211> 108
<212> DNA
<213>artificial sequence
<400> 535
gactacatgg gacatatcct gattatctta aaatgtctgc agatacatat ggaaacagca 60
tgtttttttt tcttcgccgg gaacaattat ttgtacgtcg taggttcc 108
<210> 536
<211> 108
<212> DNA
<213>artificial sequence
<400> 536
gactacatgg gacatgcctc catattggag gattggaatt ttggactaac cttacctccc 60
actgctagtt tggaagatgc ctataggttt atttacgtcg taggttcc 108
<210> 537
<211> 108
<212> DNA
<213>artificial sequence
<400> 537
gactacatgg gacattcttc atgcatggtt acatgtcttt actacactat ttgtcatttg 60
ttttacccct gaggtaatgg gaggaacctt aggtacgtcg taggttcc 108
<210> 538
<211> 108
<212> DNA
<213>artificial sequence
<400> 538
gactacatgg gacatgtaac ccatagttaa acacaggtgt gtatgtaacc gaaatgtgtt 60
ttgttaatgc atgcaagttt ctttataata acttacgtcg taggttcc 108
<210> 539
<211> 108
<212> DNA
<213>artificial sequence
<400> 539
gactacatgg gacatggtac acggcctaca acagttgtgg acgcatcccc tgctagacca 60
cctattgttg ttgaatctgt gggtcctacg gactacgtcg taggttcc 108
<210> 540
<211> 108
<212> DNA
<213>artificial sequence
<400> 540
gactacatgg gacatcctta ccaaacctga aacattaatt actgtggata atccagtctt 60
tgaggatgct gacacaactt taaccttttc acctacgtcg taggttcc 108
<210> 541
<211> 108
<212> DNA
<213>artificial sequence
<400> 541
gactacatgg gacattgcct actggtccta ccacatggcc ctatgcccct caggccccat 60
ttgacactac acatgatgtg gttatacagg gattacgtcg taggttcc 108
<210> 542
<211> 108
<212> DNA
<213>artificial sequence
<400> 542
gactacatgg gacattagtg gcaatccttt atttaataaa ttggatgaca ctraaagttc 60
cactatagct aatcaggata cagcagagga tagtacgtcg taggttcc 108
<210> 543
<211> 108
<212> DNA
<213>artificial sequence
<400> 543
gactacatgg gacatatccc ccgccaagct cagtatatgt tgctactcct agtgggtcta 60
tggtaacctc tgaggctcag ttgtttaata aactacgtcg taggttcc 108
<210> 544
<211> 108
<212> DNA
<213>artificial sequence
<400> 544
gactacatgg gacatcagga cagtttttct gctgaccttg accaattccc actgggcaga 60
aagtttttaa tgcaacttgg ggttcgtact aaatacgtcg taggttcc 108
<210> 545
<211> 108
<212> DNA
<213>artificial sequence
<400> 545
gactacatgg gacattcggt ttcctggcat gtatgagtgt tttttatatg tacatgccaa 60
aagtacattc agcaaaacac ttaatccact ggctacgtcg taggttcc 108
<210> 546
<211> 108
<212> DNA
<213>artificial sequence
<400> 546
gactacatgg gacatataaa ttgttttata acatattaca cttttgcttt gtgtagggcg 60
tgtactatgg tcgcccaccg ggcacgcaga cgatacgtcg taggttcc 108
<210> 547
<211> 108
<212> DNA
<213>artificial sequence
<400> 547
gactacatgg gacatccatg ttagtagtac ccattatacc aatccctcct ttattgaacc 60
ccctgttatt gaggttccac aaaccgggga ggttacgtcg taggttcc 108
<210> 548
<211> 108
<212> DNA
<213>artificial sequence
<400> 548
gactacatgg gacattacat tattattatg atgtgagtcc tattgcacac actgaggaaa 60
ttgaaatgca gccattattg tctgcaaata atttacgtcg taggttcc 108
<210> 549
<211> 108
<212> DNA
<213>artificial sequence
<400> 549
gactacatgg gacatatatg taaagcgcac caatatattt tatcatgcag gcagctcacg 60
tttgcttgct gttggacatc catattattc tattacgtcg taggttcc 108
<210> 550
<211> 108
<212> DNA
<213>artificial sequence
<400> 550
gactacatgg gacatctcct atacaggatg gcgacatggt tgatattggg tttggtgcca 60
tggattttaa aacattacag gaatctaaat cagtacgtcg taggttcc 108
<210> 551
<211> 108
<212> DNA
<213>artificial sequence
<400> 551
gactacatgg gacataaaca gtatgtaaga catgtagagg aatatgaatt acagtttgtg 60
tttcaactgt gtaaaatttc actgtctgca gaatacgtcg taggttcc 108
<210> 552
<211> 108
<212> DNA
<213>artificial sequence
<400> 552
gactacatgg gacatgtttt tgtatgaatg tatgttttgt ttatatgttt ctgtatgact 60
gtatgtatgt gtaatgcctg tatgtatgta atatacgtcg taggttcc 108
<210> 553
<211> 108
<212> DNA
<213>artificial sequence
<400> 553
gactacatgg gacatataag tttttattgc aaacatctgc aacacaatga tttggcttgc 60
agcatatatt ttggtagcca actatgtgtc ttgtacgtcg taggttcc 108
<210> 554
<211> 108
<212> DNA
<213>artificial sequence
<400> 554
gactacatgg gacattcgta caggatacgt gccattgccc acaactacaa catctaggcc 60
agttgaaatt cctttacaac ctaccaggcc tcctacgtcg taggttcc 108
<210> 555
<211> 108
<212> DNA
<213>artificial sequence
<400> 555
gactacatgg gacatctata caacaggtta aggttgtaga ccctgctttt ataacaacgc 60
ccacacgttt ggtaacatat gataatcctg ccttacgtcg taggttcc 108
<210> 556
<211> 108
<212> DNA
<213>artificial sequence
<400> 556
gactacatgg gacatattcc tttaaatgct ggattggata cacatcctgg ccctgatatt 60
gctttacctg tacccagtgc agaaaccatt ttttacgtcg taggttcc 108
<210> 557
<211> 108
<212> DNA
<213>artificial sequence
<400> 557
gactacatgg gacatgtgct ggtgttgagg ttggacgcgg acagccatta ggtataggca 60
caagtggtaa tccttttatg aataaacttg aagtacgtcg taggttcc 108
<210> 558
<211> 108
<212> DNA
<213>artificial sequence
<400> 558
gactacatgg gacatattcc tgatgaatta atgattaagg gtacaggcaa taccgcagcg 60
ccatccagtt gtgtttttta tcctactcct agttacgtcg taggttcc 108
<210> 559
<211> 108
<212> DNA
<213>artificial sequence
<400> 559
gactacatgg gacatggaat ctgaggatcc atatgctaaa atgacatttt gggaggtaga 60
ccttaaagaa aagttttctg cagaattaga tcatacgtcg taggttcc 108
<210> 560
<211> 108
<212> DNA
<213>artificial sequence
<400> 560
gactacatgg gacattgttt ctgctgttgt agcacaaagt ttaatttgtt ttggcttcct 60
gcaggcaact tggattgtac tcaagcactt agatacgtcg taggttcc 108
<210> 561
<211> 108
<212> DNA
<213>artificial sequence
<400> 561
gactacatgg gacatgcaca tgtcctcctg acataattcc aaaggttgaa ggcaatactt 60
tagctgacca aatattaaaa tatggtagca ttgtacgtcg taggttcc 108
<210> 562
<211> 108
<212> DNA
<213>artificial sequence
<400> 562
gactacatgg gacataccac tcattcttat gaagatatac ctcttgacac atttgtagtt 60
acaacagatc acaatagtat tgttagtagt acgtacgtcg taggttcc 108
<210> 563
<211> 108
<212> DNA
<213>artificial sequence
<400> 563
gactacatgg gacattgatg ggttatatga tgtattgtta gataatgatg tagatacaga 60
tgtagtacaa acacctaaca cttctagaaa cactacgtcg taggttcc 108
<210> 564
<211> 108
<212> DNA
<213>artificial sequence
<400> 564
gactacatgg gacatcttgc agtacctaaa gtttcaggtt tgcagtacag ggtatttaga 60
atacgtttac cagaccccaa taaatttggg ttttacgtcg taggttcc 108
<210> 565
<211> 108
<212> DNA
<213>artificial sequence
<400> 565
gactacatgg gacatatgta aatatccaga ttatttaggc atggctgcag atccctatgg 60
tgattctatg tggttttata ttcgtaggga acatacgtcg taggttcc 108
<210> 566
<211> 108
<212> DNA
<213>artificial sequence
<400> 566
gactacatgg gacatctatg agttctagta tattagaaca gtggaatttt ggtcttacac 60
caccgccatc tggtacttta gaagaaacat atatacgtcg taggttcc 108
<210> 567
<211> 108
<212> DNA
<213>artificial sequence
<400> 567
gactacatgg gacatactat atatgttgtg tgagtggttt atgctagcca ctgtgtctcc 60
attttgttca ttcgccattt tatgtatttt aagtacgtcg taggttcc 108
<210> 568
<211> 108
<212> DNA
<213>artificial sequence
<400> 568
gactacatgg gacattttgt ctgctttaat gtgccgctat tgcagtctgc ctctgtgttt 60
gcctcgtgta tattacttat acttttattt tggtacgtcg taggttcc 108
<210> 569
<211> 108
<212> DNA
<213>artificial sequence
<400> 569
gactacatgg gacatctatt gttgtagaat cggtaggccc cactgaccct tcaattgtta 60
cattggttga agaatccagt gttattgagt ctgtacgtcg taggttcc 108
<210> 570
<211> 108
<212> DNA
<213>artificial sequence
<400> 570
gactacatgg gacatcaaaa tgctgacaca acgttaacct tttcaccatc aggtgtggca 60
cctgatcctg attttttaga cattgtggct ttatacgtcg taggttcc 108
<210> 571
<211> 108
<212> DNA
<213>artificial sequence
<400> 571
gactacatgg gacattgata ccacccatga tgtagttata cagggctcca catttgcgtt 60
atggcctgtt tattttttga aacgtaggcg tcgtacgtcg taggttcc 108
<210> 572
<211> 108
<212> DNA
<213>artificial sequence
<400> 572
gactacatgg gacatgctat tcaggatact gccccggaca gtagggataa tgtttcagtg 60
gatcctaaac aaacacagtt atgcattatt ggctacgtcg taggttcc 108
<210> 573
<211> 108
<212> DNA
<213>artificial sequence
<400> 573
gactacatgg gacatttcag aggctcaatt gtttaataag ccatattggc tgcaacgtgc 60
ccagggacat aataatggca tctgttggaa caatacgtcg taggttcc 108
<210> 574
<211> 108
<212> DNA
<213>artificial sequence
<400> 574
gactacatgg gacattaatg caggttgggg tccgtactaa accgcctgta tcctctaaaa 60
aacgctctgc ttctactaca tctacctcag ccctacgtcg taggttcc 108
<210> 575
<211> 108
<212> DNA
<213>artificial sequence
<400> 575
gactacatgg gacataagca tattcagcag aactgttaat cctttggcat agtgccgttt 60
cctgtgttta atgttttttt gttttataca ctatacgtcg taggttcc 108
<210> 576
<211> 108
<212> DNA
<213>artificial sequence
<400> 576
gactacatgg gacatgatct acaaggcgca aacgtgcctc tgcaacacaa ctatatcaaa 60
catgcaaggc ggcaggcacc tgtcccccgg atgtacgtcg taggttcc 108
<210> 577
<211> 108
<212> DNA
<213>artificial sequence
<400> 577
gactacatgg gacatttttc atcttctgaa gcctctggtc atttaatatt ttctacaccc 60
actattagta cacattcata tgaggacatt ccttacgtcg taggttcc 108
<210> 578
<211> 108
<212> DNA
<213>artificial sequence
<400> 578
gactacatgg gacatactac agccattatc tcgccctgtg tcttctgctt cccattctat 60
taatgatggt ttatatgatg tatatatgga tcctacgtcg taggttcc 108
<210> 579
<211> 108
<212> DNA
<213>artificial sequence
<400> 579
gactacatgg gacatggcca tccttacttt tccattccta atccctccaa cactaaaaag 60
gtgttagtgc ccaaggtgtc aggtttgcag tattacgtcg taggttcc 108
<210> 580
<211> 108
<212> DNA
<213>artificial sequence
<400> 580
gactacatgg gacatatctt tgcaggccaa taaaagtgat gttccgttag atatatgtac 60
cagtatttgt aaatatcctg attatcttgg tattacgtcg taggttcc 108
<210> 581
<211> 108
<212> DNA
<213>artificial sequence
<400> 581
gactacatgg gacatgcaaa atatccctta ctgcaaatgt tatgcaatac atacacacca 60
tgaatccaga tatattagag gactggcaat ttgtacgtcg taggttcc 108
<210> 582
<211> 108
<212> DNA
<213>artificial sequence
<400> 582
gactacatgg gacatttaac tatattgttg tgtcgtgtgg ttgcacccaa tgagtaaggt 60
actgtcccta tatccctgcc catgccatgc ccttacgtcg taggttcc 108
<210> 583
<211> 108
<212> DNA
<213>artificial sequence
<400> 583
gactacatgg gacatggttt tataggttcc cccttacggg tatttttggc atatcttata 60
tttttgtacc tacctatgat gtgtattcat ttatacgtcg taggttcc 108
<210> 584
<211> 108
<212> DNA
<213>artificial sequence
<400> 584
gactacatgg gacattgata ctgtagggcc cctagactct tctattgtgt ctatgataga 60
ggaaacaagt tttattgagt caggtgcacc tgctacgtcg taggttcc 108
<210> 585
<211> 108
<212> DNA
<213>artificial sequence
<400> 585
gactacatgg gacataacct gatgaaacac tttactttca acaccaagac atttcacctg 60
cacctgaccc tgacttttta gacatagttg cattacgtcg taggttcc 108
<210> 586
<211> 108
<212> DNA
<213>artificial sequence
<400> 586
gactacatgg gacatccctt taacaatgta cttgtttatg gtagtgattt tattttacac 60
cctagttatt ttttacgtcg caggcgtaaa cggtacgtcg taggttcc 108
<210> 587
<211> 108
<212> DNA
<213>artificial sequence
<400> 587
gactacatgg gacataatac cctagccagc ctggtactga taatagggaa tgcttgtcta 60
tggatgctaa gcagacccag ctatgtatta ttgtacgtcg taggttcc 108
<210> 588
<211> 108
<212> DNA
<213>artificial sequence
<400> 588
gactacatgg gacattcctc agaatctcaa ttatttaata aaccatattg gttacaacgc 60
gcacaaggtc ataacaatgg tatatgctgg ggttacgtcg taggttcc 108
<210> 589
<211> 108
<212> DNA
<213>artificial sequence
<400> 589
gactacatgg gacattttat tgcaggcagg ttttacagct aaacctaaac taaaacgttc 60
ttcaccttct tcgtcctctt cctcctctgc aaatacgtcg taggttcc 108
<210> 590
<211> 108
<212> DNA
<213>artificial sequence
<400> 590
gactacatgg gacatgtgca atgcctttac ttgctgttgc tctcccgcct aaattgactc 60
atagtattgc agtgcaacac gcccttttac atgtacgtcg taggttcc 108
<210> 591
<211> 108
<212> DNA
<213>artificial sequence
<400> 591
gactacatgg gacataatca ggcacatgtc cgcctgatgt tgttaataag gtggagggta 60
ccacactggc tgataggttt ttacaatggg ctatacgtcg taggttcc 108
<210> 592
<211> 108
<212> DNA
<213>artificial sequence
<400> 592
gactacatgg gacatcatgg atatgaagaa attcctatgc aggtttttgc ctcacatgga 60
acaggcacag aacctattag tagtactcct gtttacgtcg taggttcc 108
<210> 593
<211> 108
<212> DNA
<213>artificial sequence
<400> 593
gactacatgg gacattaatg caatgttaca tactacttct catacaggtt ctacaggacc 60
taggtcccat ctttcatttc cttctatacc ttctacgtcg taggttcc 108
<210> 594
<211> 108
<212> DNA
<213>artificial sequence
<400> 594
gactacatgg gacatcctaa ggtgtctgca tatcagtata gggtatttag ggtatcccta 60
cctgatccta ataagtttgg ccttccggat ccttacgtcg taggttcc 108
<210> 595
<211> 108
<212> DNA
<213>artificial sequence
<400> 595
gactacatgg gacattcctg attatttgca gatgtctgct gatgtatatg gggacagtat 60
gtttttttgt ttgcgcaagg aacagttgtt tgctacgtcg taggttcc 108
<210> 596
<211> 108
<212> DNA
<213>artificial sequence
<400> 596
gactacatgg gacatcaatt ttggacaatt ggaatatagg agttacccct ccaccatctg 60
caagcttggt ggacacgtat aggtatttac aattacgtcg taggttcc 108
<210> 597
<211> 108
<212> DNA
<213>artificial sequence
<400> 597
gactacatgg gacattatgt gtatgtttta gaagtatgtg tgtatgtatg tttttgttaa 60
taaagtatgt atggaggttt catttgtggt tgctacgtcg taggttcc 108
<210> 598
<211> 108
<212> DNA
<213>artificial sequence
<400> 598
gactacatgg gacatcatag ttggcctgta taacagcttt tacatccaag aatgtgtcgt 60
ttggtgcaag ttatattttg tgactaatat ttttacgtcg taggttcc 108
<210> 599
<211> 108
<212> DNA
<213>artificial sequence
<400> 599
gactacatgg gacatgtttt atagagtcag gtatacctgg tcctacatct atagtgcctt 60
ctacttcagg gtttgatatt acaacttctg taatacgtcg taggttcc 108
<210> 600
<211> 108
<212> DNA
<213>artificial sequence
<400> 600
gactacatgg gacatgattc tgatttttta gatattgtaa aattacatag gcctgcttta 60
acctctagaa aaacaggcat acgtgttagt agatacgtcg taggttcc 108
<210> 601
<211> 108
<212> DNA
<213>artificial sequence
<400> 601
gactacatgg gacattttta tattacaccc tagttattat ttgttaaagc gcaaacgtaa 60
acgtctgtca tattctttta cagatgtggc gactacgtcg taggttcc 108
<210> 602
<211> 108
<212> DNA
<213>artificial sequence
<400> 602
gactacatgg gacatatgtc agtggattat aaacaaacac agttgtgtat tttaggttgt 60
aggcctccct taggggaaca ttggggtcca ggctacgtcg taggttcc 108
<210> 603
<211> 108
<212> DNA
<213>artificial sequence
<400> 603
gactacatgg gacatgcaaa aggcacaggg acaaaataat ggtatttgtt ggcataatca 60
attattttta actgttgtag atactactag aagtacgtcg taggttcc 108
<210> 604
<211> 108
<212> DNA
<213>artificial sequence
<400> 604
gactacatgg gacatctaag ttacaagctt ctaaacgttc tgcatctgct accacaagtg 60
ccacacctaa gaaaaaacgt gctaaacgta ttttacgtcg taggttcc 108
<210> 605
<211> 108
<212> DNA
<213>artificial sequence
<400> 605
gactacatgg gacataggtt ataaaggtaa aaggcgccac ctggcagtta ctcatttgtc 60
tgcaattatt taaacaatgt cttgcacaca cattacgtcg taggttcc 108
<210> 606
<211> 108
<212> DNA
<213>artificial sequence
<400> 606
gactacatgg gacattttga tttacagttt gtttttcagt tatgtaaaat tagtttaact 60
actgaggtaa tgacatatat acattctatg aattacgtcg taggttcc 108
<210> 607
<211> 108
<212> DNA
<213>artificial sequence
<400> 607
gactacatgg gacattagtt tgggaatatt tttaggaggc cttggaatag gtactggtag 60
tggcacaggg gggcgtaccg gctatattcc atttacgtcg taggttcc 108
<210> 608
<211> 108
<212> DNA
<213>artificial sequence
<400> 608
gactacatgg gacatttcct ggtgttaggc gtgtggcagg acctagatta tatagtcaag 60
cttatcaaca agttaaaata actaattcag acttacgtcg taggttcc 108
<210> 609
<211> 108
<212> DNA
<213>artificial sequence
<400> 609
gactacatgg gacattctcg ttatagtaat actactattc ctttagcaac atcttgggat 60
gttcctgttc atacagggcc tgacatgaca ttatacgtcg taggttcc 108
<210> 610
<211> 108
<212> DNA
<213>artificial sequence
<400> 610
gactacatgg gacatatcct gaaacacaac gtttagtatg ggcctgtgtt ggcatggagg 60
taggtcgtgg gcagccttta ggtgttagcc ttatacgtcg taggttcc 108
<210> 611
<211> 108
<212> DNA
<213>artificial sequence
<400> 611
gactacatgg gacatcattt ttggaacagg ggaggcacta taggagatgc agtaccggaa 60
acattatata taaaaggtac taatgatagg gcatacgtcg taggttcc 108
<210> 612
<211> 108
<212> DNA
<213>artificial sequence
<400> 612
gactacatgg gacatcatat gaaaaattaa agttttggaa tgtggatctt aaagaacatt 60
tttcatctga tttagatcag tttcctttag gactacgtcg taggttcc 108
<210> 613
<211> 108
<212> DNA
<213>artificial sequence
<400> 613
gactacatgg gacatcaagc ctccatttta ctatgcaacc gatatcggtt gcctttgaca 60
cacctaacat tatacattct tattgttaca ttatacgtcg taggttcc 108
<210> 614
<211> 108
<212> DNA
<213>artificial sequence
<400> 614
gactacatgg gacatgcaga taaaatttta cagtggtcca gccttggtat atttttgggt 60
ggtcttggga taggtactgg tagtggtact gggtacgtcg taggttcc 108
<210> 615
<211> 108
<212> DNA
<213>artificial sequence
<400> 615
gactacatgg gacattgaac caattagtag taccccattg cctaatatta ggcgtgtggc 60
aggtccccgc ctttatagta aggctaatca acatacgtcg taggttcc 108
<210> 616
<211> 108
<212> DNA
<213>artificial sequence
<400> 616
gactacatgg gacatctact gtatcgtcag tctcttctta tggtaacctt actgttcctt 60
taacttcctc atgggatgtg cctgtatata cagtacgtcg taggttcc 108
<210> 617
<211> 108
<212> DNA
<213>artificial sequence
<400> 617
gactacatgg gacattgact ctactattta caatcccgaa acacaacgtc ttgtgtgggc 60
ctgtgttggt gttgaaatag gccgtggaca acctacgtcg taggttcc 108
<210> 618
<211> 108
<212> DNA
<213>artificial sequence
<400> 618
gactacatgg gacataacaa ctgtttgcta ggcatttttg gaacagagct ggaacaatgg 60
gtgacactat tccacaggag ttatatatta aagtacgtcg taggttcc 108
<210> 619
<211> 108
<212> DNA
<213>artificial sequence
<400> 619
gactacatgg gacatcgctt tgtacagtct gttgctataa cctgtcaaaa ggatactact 60
cccgctgaga aaaaggatcc ttatgataac ttatacgtcg taggttcc 108
<210> 620
<211> 108
<212> DNA
<213>artificial sequence
<400> 620
gactacatgg gacatcattt tatattacaa ttctccattt taatgtgcaa ccgttttcgg 60
ttgcccttgg cttacctttg tacattctgg cactacgtcg taggttcc 108
<210> 621
<211> 108
<212> DNA
<213>artificial sequence
<400> 621
gactacatgg gacattaaca ataatgttat ataaaaatgg gtgtaaccga aaacggttgc 60
aaccgaaact ggtgtatata aaagacctgc aaatacgtcg taggttcc 108
<210> 622
<211> 50
<212> DNA
<213>artificial sequence
<400> 622
aatgatacgg cgaccaccga gatctacact ctttccctac acgacgctct 50
<210> 623
<211> 50
<212> DNA
<213>artificial sequence
<400> 623
caagcagaag acggcatacg agatcggtct cggcattcct gctgaaccgc 50
<210> 624
<211> 21
<212> DNA
<213>artificial sequence
<400> 624
aatgatacgg cgaccaccga g 21
<210> 625
<211> 21
<212> DNA
<213>artificial sequence
<400> 625
caagcagaag acggcatacg a 21

Claims (8)

1. reagent set is made of 621 probes, the sequence of 621 articles of probes is respectively of sequence 1-621 in sequence table 16-93.
2. reagent set is made of 621 probes, the sequence of 621 probes is sequence 1-621 in sequence table.
3. reagent set according to claim 1 or 2, it is characterised in that: 5 ' of every probe in the reagent set End label has.
4. the system for detecting human papilloma virus, including any reagent set in claim 1-3.
5. system according to claim 4, it is characterised in that: the system also includes M1, the M1 be harvesting buffer, Hybridization buffer and/or combination buffer；
The harvesting buffer is made of solvent and solute, and the solvent is water, solute behaviour cot-1 DNA, salmon essence DNA, primer 1 and primer 2, the primer 1 are single stranded DNA shown in sequence 622 in sequence table, and the primer 2 is in sequence table Single stranded DNA shown in sequence 623；
The hybridization buffer is made of solvent and solute, and the solvent is Tris-HCl, the solute and its in the hybridization Concentration in buffer is respectively 1.25M NaCl, 0.125 M sodium citrate, 0.1g/100mL BSA, and volumn concentration is The Tween20 of 5%-10%；Wherein, the Tris-HCl is made of solvent and solute, and the solvent is water, and the solute is three hydroxyls Aminomethane and HCl for adjusting pH, the concentration of trishydroxymethylaminomethane are 50mM-200mM, pH 7.6；
The combination buffer is made of solvent and solute, and the solvent is Tris-HCl, and the solute and its concentration are respectively 1M NaCl, 1 mM EDTA；The Tris-HCl is made of solvent and solute, and the solvent is water, and the solute is three hydroxyl first Base aminomethane and HCl for adjusting pH, the concentration of trishydroxymethylaminomethane are 10 mM, pH 7.5.
6. system according to claim 5, it is characterised in that: the content of solute described in the harvesting buffer is respectively The people cot-1 DNA 35% of Invitrogen, the salmon sperm dna 15% of Invitrogen, 1 0.5nmol/ μ l of the primer and institute State primer 2 0.5nmol/ μ l；
In the hybridization buffer, the solvent is Tris-HCl, the solute and its concentration in the hybridization buffer Respectively 1.25M NaCl, 0.125 M sodium citrate, 0.1 g/100mL BSA, 7%Tween20；The Tris-HCl is by molten Agent and solute composition, the solvent are water, and the solute is trishydroxymethylaminomethane and the HCl for adjusting pH, three hydroxyl first The concentration of base aminomethane is 100mM, pH 7.6；
The percentage is volumn concentration.
7. system according to claim 5 or 6, it is characterised in that: the system also includes M2, the M2 be rinsing liquid 1, At least one of rinsing liquid 2, NaOH solution, Tris-HCl buffer, PCR reaction solution and TE buffer；
The rinsing liquid 1 is made of solvent and solute, and the solvent is 1 × SSC solution, and the solute is SDS, and SDS is described Mass percent concentration in rinsing liquid 1 is 0.1%；1 × SSC solution is made of solvent and solute, and the solvent is water, The solute and its concentration in 1 × SSC solution are respectively NaCl 175g/L and trisodium citrate 88g/L, pH 7.4；
The rinsing liquid 2 is made of solvent and solute, and the solvent is 0.1 × SSC solution, and the solute is SDS, and SDS is in institute Stating the mass percent concentration in rinsing liquid 2 is 0.1%；Wherein, 0.1 × SSC solution is to dilute 1 × SSC solution 10 times of obtained solution；
The NaOH solution is made of solvent and solute, and the solvent is water, the solute and dense in the NaOH solution Degree is 0.1M NaOH；
The Tris-HCl buffer is made of solvent and solute, and the solvent is water, and the solute is trihydroxy methyl amino first Alkane and HCl for adjusting pH, the concentration of trishydroxymethylaminomethane are 1M, pH 7.5；
The PCR reaction solution is made of solvent and solute, the solvent be 1 times of concentration Phusion buffer, the solute and Its concentration in the PCR reaction solution be respectively 0.2mM dATP, 0.2mM dTTP, 0.2mM dCTP, 0.2mM dGTP, 2.5pmol primer 3,2.5pmol primer 4,0.05U/ μ l Hotstart Phusion enzyme, 5%DMSO；Wherein, described 1 times it is dense The Phusion buffer of degree is made of solvent and solute, and the solvent is 50 mM Tris-HCl, the solute and its described Concentration in the Phusion buffer of 1 times of concentration is respectively 4mM MgCl2, 500 mM KCl, 0.8% Nonidet P40；Institute It states 50 mM Tris-HCl to be made of solvent and solute, the solvent is water, and the solute is trishydroxymethylaminomethane and use In the HCl for adjusting pH, the concentration of trishydroxymethylaminomethane is 50 mM, pH 8.8；The sequence of the primer 3 and the primer 4 Last nucleotide of sequence 624 and 625 in column respectively sequence table, the primer 3 and the primer 4 has thio repair Decorations；
The TE buffer is made of solvent and solute, and the solvent is water, and the solute is trishydroxymethylaminomethane, EDTA With the HCl for adjusting pH, the concentration of trishydroxymethylaminomethane is 10mM, and the concentration of EDTA is 1mM, pH 8.0；
The percentage is volumn concentration.
8. following any applications of reagent set as claimed in claim 1 or 2:
Y1) the application in preparation detection Human Papillomavirus DNA group serial products；
Y2 it) is applied in preparation detection typing of human papillomavirus product；
Y3) the application in preparation detection human papillomavirus HPV DNA in human genome in insertion point product；
Y4) the application in preparation detection HPV copy number product.
CN201810580442.6A 2018-06-07 2018-06-07 For detecting the reagent set and method of human papilloma virus Active CN108676797B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201810580442.6A CN108676797B (en) 2018-06-07 2018-06-07 For detecting the reagent set and method of human papilloma virus

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201810580442.6A CN108676797B (en) 2018-06-07 2018-06-07 For detecting the reagent set and method of human papilloma virus

Publications (2)

Publication Number Publication Date
CN108676797A CN108676797A (en) 2018-10-19
CN108676797B true CN108676797B (en) 2019-04-26

Family

ID=63810444

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201810580442.6A Active CN108676797B (en) 2018-06-07 2018-06-07 For detecting the reagent set and method of human papilloma virus

Country Status (1)

Country Link
CN (1) CN108676797B (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112195287B (en) * 2020-11-12 2022-03-01 武汉凯德维斯生物技术有限公司 Probe group for human papilloma virus HPV typing and integration detection and kit thereof

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100633525B1 (en) * 2004-10-04 2006-10-16 굿젠 주식회사 Probe Of Human Papillomavirus Oligonucleotide Microarray And Genotyping Kit Comprising The Same And Genotyping Method For Human Papillomavirus Using The Same
KR100702415B1 (en) * 2006-03-03 2007-04-09 안웅식 Kits and method for detecting human papilloma virus with oligo nucleotide bead array
WO2011088573A1 (en) * 2010-01-19 2011-07-28 Her Majesty The Queen In Right Of Canada As Represented By The Minister Of Health Set of probes for the detection and typing of 46 human papillomavirus mucosal types
JP6103941B2 (en) * 2010-01-29 2017-03-29 キアジェン ゲイサーズバーグ インコーポレイテッド Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids
CN101818213B (en) * 2010-04-20 2012-02-08 济南艾迪康医学检验中心有限公司 Gene chip and kit for detecting human papillomavirus (HPV)
KR101239387B1 (en) * 2010-06-17 2013-03-11 문우철 Dna chip for genotyping of human papilloma virus, kit comprising the same, and method for genotyping hpv
WO2012116220A2 (en) * 2011-02-24 2012-08-30 Qiagen Gaithersburg, Inc. Materials and methods for detection of hpv nucleic acid
CN105400901A (en) * 2014-08-20 2016-03-16 北京百诺奇生物科技有限公司 Kit for detecting human papilloma virus and application of kit
CN107735500A (en) * 2015-04-20 2018-02-23 宾夕法尼亚大学董事会 For detecting the grand genome composition and method of breast cancer
CN106555010A (en) * 2015-09-30 2017-04-05 江苏博铼生技医疗科技有限公司 Human papilloma virus's detection kit and detection method

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
国家食品药品监督管理局通告2015年第93号 附件3人乳头瘤病毒(HPV)核酸检测及基因分型试剂技术审查指导原则;国家食品药品监督管理局;《国家食品药品监督管理局通告》;20151126;第1-32页

Also Published As

Publication number Publication date
CN108676797A (en) 2018-10-19

Similar Documents

Publication Publication Date Title
CN110423844B (en) Methods and compositions for detecting BK virus
DK2644713T3 (en) A Method for Diagnosing Neoplasms II
ES2374954T3 (en) GENETIC VARIATIONS ASSOCIATED WITH TUMORS.
KR102023584B1 (en) PREDICTING GASTROENTEROPANCREATIC NEUROENDOCRINE NEOPLASMS (GEP-NENs)
CN106119405A (en) The prognostic markers thing of a kind of pulmonary carcinoma, the method using this labelling prediction lung cancer for prognosis and application
KR20140006898A (en) Colon cancer gene expression signatures and methods of use
CA2442820A1 (en) Microarray gene expression profiling in clear cell renal cell carcinoma: prognosis and drug target identification
CN112195287B (en) Probe group for human papilloma virus HPV typing and integration detection and kit thereof
CN109423515A (en) One group of gene marker and its application for liver cancer detection
CN109593828A (en) A kind of construction method and its kit in the genetic test library of heredity arrhythmia cordis
CN108676797B (en) For detecting the reagent set and method of human papilloma virus
CN109593849B (en) Plasma LncRNA marker related to colorectal cancer and application thereof
CN112391466A (en) Methylation biomarker for detecting breast cancer or combination and application thereof
CN112266983B (en) Probe set for detecting human papilloma viruses HPV16 and HPV18 and kit thereof
KR102510442B1 (en) Probe set for detecting trace amount of sfts virus, adeno virus and covid-19 virus, method for whole genome sequencing and detecting sfts virus, adeno virus and covid-19 virus using the same
CN107338247B (en) SNP molecular marker associated with upland cotton fiber strength and application thereof
CN107223162A (en) New RNA biomarkers label for diagnosis of prostate cancer
KR20200004977A (en) Probes for detecting gene mutations for cancer, method for producing the same and method for detecting cancer using thereof
CN112522439B (en) Probe set for detecting HPV (human papilloma Virus) typing and integration of 5 human papilloma viruses and kit thereof
CN111172270B (en) Whole blood transcription gene marker for grading cervical squamous intraepithelial lesions and application thereof
TW201827602A (en) Primary site of metastatic cancer identification method and system thereof
CN114032298B (en) Probe set for detecting genetic bilirubin metabolic abnormality and intrahepatic cholestasis related gene variation, kit and application thereof
CN112266982A (en) Probe for detecting human papilloma virus HPV45 and kit thereof
KR102115938B1 (en) Single nucleotide polymorphism for predicting the risk factor of metabolic syndrome and the use thereof
CN108913761A (en) A kind of kit for screening genetic liver

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant
TR01 Transfer of patent right
TR01 Transfer of patent right

Effective date of registration: 20211213

Address after: 400020 4-2, 5-2 and 6-2, building 1, No. 6, Gangcheng East Ring Road, Jiangbei District, Chongqing

Patentee after: Chongqing maijinuo medical laboratory Co.,Ltd.

Address before: 400000 1-2, 2-2, 3-2, 4-2, 5-2, 6-2, building 1, No. 6, Gangcheng East Ring Road, Jiangbei District, Chongqing

Patentee before: MYGENOSTICS (CHONGQING) GENE TECHNOLOGY CO.,LTD.