CN106119405A - The prognostic markers thing of a kind of pulmonary carcinoma, the method using this labelling prediction lung cancer for prognosis and application - Google Patents

Abstract

The invention discloses the prognostic markers thing of a kind of pulmonary carcinoma, the method using this labelling prediction lung cancer for prognosis and application, described label includes the following assortment of genes: RP11 89K21.1, CDH17, GRIA1, CENPF, AP000438.2, HMMR, RP11 434D9.1, ADH1B, TPX2, OR7E47P.Instant invention overcomes lung cancer therapy reaction and prognosis difference is big, prognosis is caused to be forbidden to determine convenient difficult point, by label and detection label, rapidly, lung cancer for prognosis is predicted accurately, easily, achieve the hierarchical classification of height at the bottom of Lung Cancer Risk from molecular level, significantly by high low-risk patient separately, thus instruct individualized treatment, there is higher clinical value, saved medical treatment cost.

Description

A kind of prognostic markers thing of pulmonary carcinoma, use this labelling prediction lung cancer for prognosis method and Application

Technical field

The invention belongs to biomedical sector, be specifically related to the prognostic markers thing of a kind of pulmonary carcinoma and use this labelling prediction lung The method of cancer prognosis and application.

Background technology

It is the fastest, to one of maximum malignant tumor of population health and life threat that pulmonary carcinoma is that M & M increases. Immediate and mid-term many countries all report that the M & M of pulmonary carcinoma the most substantially increases, and male lung cancer M & M is equal Accounting for first of all malignant tumor, women sickness rate accounts for second, and mortality rate accounts for second.

In recent years, along with emerging in large numbers of large scale sequencing and chip data, present a lot by molecular level prediction disease The case of people's prognosis.In breast carcinoma, breast carcinoma 21 gene test (OncotypeDx) refers to detect in breast tumor tissue 21 Individual heterogeneic expression, this detection can provide the therapeutic effect prediction of individuation and the pre-of 10 years risks of recurrence Survey.By detection 21 genes of detection, observe the interaction between them to judge tumor characteristic, thus measurable breast carcinoma Recurrence index and accept the benefit ratio of chemotherapy.In the U.S., 21 gene tests have rapidly become breast cancer treatment standardization program, Have more than 7500 doctors to use up to more than 65000 times.Up to now, this detection is carried out the nearlyest 9 years in American-European multiple countries, Benefit crowd reaches 150,000.

Pulmonary carcinoma is one of modal malignant tumor, and mortality rate has been in first of cancer mortality.Pulmonary carcinoma includes minicell The multiple histological type such as pulmonary carcinoma, adenocarcinoma of lung, lung squamous cancer, wherein adenocarcinoma of lung is most commonly seen Lung Cancer Types, and its feature is easy Postoperative metastasis occur, grade malignancy is high, poor prognosis.Obtained how long pulmonary carcinoma can live actually？This problem depends on cancer Of science type, neoplasm staging, patient age, health basic condition and the most reasonably treatment etc. factor can be obtained.Lung The histopathology classification of cancer and clinical stages are the clinical prognosis indexs of current pulmonary carcinoma, but lung cancer therapy reaction and poor prognosis Not big, therefore it is carried out the inevitable requirement that molecule parting is pulmonary carcinoma individualized treatment.In pulmonary carcinoma, there is presently no picture The such technique of gene detection of OncotypeDx.If we also can be by detecting the expression of gene, it was predicted that the prognosis of patient, this To bring glad tidings for lung cancer patient.

It is desirable to find out the prognostic gene of pulmonary carcinoma, subject matter have some: the gene of (1) a large amount of lung cancer sample Express data.The sample of cancer patient then becomes less easily accessible to, even if taking sample, the order-checking expense of great number is also one and no small opens ?.(2) follow up data of lung cancer patient.The reason causing follow up data to lose mainly has Medical Staff to following up a case by regular visits to weigh not Depending on, not understanding of following up a case by regular visits to, some patients are difficult to get in touch with by patient.(3) statistical models.The data of life cycle is typically disobeyed Normal distribution, conventional statistical method is inapplicable, and by special statistical method.Additionally, a lot of patients definite in following up a case by regular visits to Life span is unknown, only knows that the life span of patient is more than certain time.But these censored datas can not be left out, because this is not only Have lost data, and bias can be caused.

Summary of the invention

The prognostic markers thing that it is an object of the invention to provide a kind of pulmonary carcinoma, the method using this labelling prediction lung cancer for prognosis And application.

The technical solution used in the present invention is:

A kind of prognostic markers thing of pulmonary carcinoma, described label include the following assortment of genes: RP11-89K21.1, CDH17, GRIA1, CENPF, AP000438.2, HMMR, RP11-434D9.1, ADH1B, TPX2, OR7E47P, the sequence of described gene is followed successively by SEQ ID NO:1～10.

Preferably, at least one gene during described label also includes following gene: PLK1, HABP2, PBK, GPR37, NEK2, RP11-497G19.2, SLC2A1, PLOD2, ECT2, SPC25, the sequence of described gene be followed successively by SEQ ID NO:11～ 20。

Preferably, at least one gene during described label also includes following gene: RP5-839B4.8, SKA3, C20orf197, LYPD3, E2F7, CPS1, ABCA3, TICRR, CDKN3, VIPR1, MTL5, FAM64A, SFTPD, BUB1, described The sequence of gene is followed successively by SEQ ID NO:21～34.

Preferably, described pulmonary carcinoma is adenocarcinoma of lung.

The method for prediction of prognosis of a kind of pulmonary carcinoma, comprises the following steps:

1) gather sample from patients with lung cancer, detect and obtain the expression of the prognostic markers thing of pulmonary carcinoma；

2) expression obtained is applied to the prognostic predictive model of pulmonary carcinoma, to obtain lung cancer for prognosis value-at-risk；

3) obtained lung cancer for prognosis value-at-risk is compared with reference value, determine the prognosis of described patient；

The prognostic markers thing of described pulmonary carcinoma is described in any one of Claims 1 to 4；

The prognostic predictive model of described pulmonary carcinoma is:

S=∑Pi * Ci

Wherein, S is lung cancer for prognosis value-at-risk, and Pi is the quantitative expression value of i-th bit gene, and Ci represents the COX of i-th bit gene and returns Return coefficient, i=1,2,3,4 ... n, n are the sum of the prognostic markers thing of pulmonary carcinoma.

Preferably, the Index for diagnosis method of patient:

Compare with reference value according to obtained lung cancer for prognosis value-at-risk, if less than or equal to reference value, detected patient just belongs to Low-risk, if greater than reference value, detection patient just belongs to low-risk.

Preferably, in the prognostic predictive model of described pulmonary carcinoma, COX regression coefficient determines by the following method:

1) the multidigit patients with lung cancer collected is about the transcript profile data of the prognostic markers thing of pulmonary carcinoma and clinical Survival data；

2) use the prognostic markers thing of the multifactor above-mentioned pulmonary carcinoma of COX model stepwise regression analysis, obtain COX regression coefficient；

Described reference value determines by the following method: turning according to the multidigit patients with lung cancer of gained COX regression coefficient and above-mentioned collection Record group data, calculate multidigit patients with lung cancer lung cancer for prognosis value-at-risk by the prognostic predictive model of pulmonary carcinoma, take multidigit patients with lung cancer lung The median of cancer prognostic risk value is as reference value.

Preferably, the prognostic markers thing of pulmonary carcinoma include the following assortment of genes: RP11-89K21.1, CDH17, GRIA1, CENPF, AP000438.2, HMMR, RP11-434D9.1, ADH1B, TPX2, OR7E47P, the sequence of described gene is followed successively by SEQ ID NO:1～10；Preferably, in the method for prediction of prognosis of pulmonary carcinoma, the COX regression coefficient that described detection gene pairs is answered is selected from down Table:

In the method for prediction of prognosis of pulmonary carcinoma, described reference value is 1.02.

Preferably, the prognostic markers thing of pulmonary carcinoma include the following assortment of genes: RP11-89K21.1, CDH17, GRIA1, CENPF, AP000438.2, HMMR, RP11-434D9.1, ADH1B, TPX2, OR7E47P, GPR37, HABP2, described gene Sequence is followed successively by SEQ ID NO:1～12；Preferably, in the method for prediction of prognosis of pulmonary carcinoma, the COX that described detection gene pairs is answered returns Coefficient is returned to be selected from following table:

In the method for prediction of prognosis of pulmonary carcinoma, described reference value is 0.986.

Preferably, the prognostic markers thing of pulmonary carcinoma include the following assortment of genes: RP11-89K21.1, CDH17, GRIA1, CENPF、AP000438.2、HMMR、RP11-434D9.1、ADH1B、TPX2、OR7E47P、PLK1、HABP2、PBK、GPR37、 NEK2、RP11-497G19.2、SLC2A1、PLOD2、ECT2、SPC25、RP5-839B4.8、SKA3、C20orf197、LYPD3、 E2F7, CPS1, ABCA3, TICRR, CDKN3, VIPR1, MTL5, FAM64A, SFTPD, BUB1, the sequence of described gene is followed successively by SEQ ID NO:1～34；Preferably, in the method for prediction of prognosis of pulmonary carcinoma, the COX regression coefficient that described detection gene pairs is answered is selected from Following table:

In the method for prediction of prognosis of pulmonary carcinoma, described reference value is 0.979.

A kind of test kit for assessing lung cancer for prognosis life cycle, it include the mRNA for detecting above-mentioned label or The reagent of protein expression level.

Preferably, the reagent of the mrna expression level for detecting label is the primer being combined with described gene specific And/or probe；It is the special antibody of the albumen to described gene code for detecting the reagent of the protein expression level of label.

The invention has the beneficial effects as follows:

Instant invention overcomes lung cancer therapy reaction and prognosis difference is big, cause prognosis to be forbidden to determine convenient difficult point, pass through labelling Thing and the expression of detection label, predict lung cancer for prognosis rapidly, accurately, easily, achieve Lung Cancer Risk from molecular level The hierarchical classification of end height, significantly by high low-risk patient separately, thus instructs individualized treatment, has higher clinic Using value, has saved medical treatment cost.

The present invention finds the gene relevant to pulmonary carcinoma existence, and establishes the mathematical modulo before these genes and life span Type.Model in the present invention does pulmonary carcinoma survival analysis, and solving the life span of patient has a lot of censored data, and life cycle The problem that data typically disobeys normal distribution.

Accompanying drawing explanation

The present invention is further illustrated below in conjunction with the accompanying drawings.

Fig. 1 is that the high low-risk survival curve of embodiment 2 compares；

Fig. 2 is the ROC curve figure of embodiment 2；

Fig. 3 is that the high low-risk survival curve of embodiment 3 compares；

Fig. 4 is the ROC curve figure of embodiment 3；

Fig. 5 is that the high low-risk survival curve of embodiment 4 compares；

Fig. 6 is the ROC curve figure of embodiment 4.

Detailed description of the invention

The foundation of the prognostic predictive model of prognostic markers thing and pulmonary carcinoma selected by the present invention is all to screen by the following method 's.The method for building up of the prognostic predictive model of pulmonary carcinoma, comprises the following steps:

1) the transcript profile data of patients with lung cancer and clinical Survival data are collected；

2) DESeq is used to analyze the gene differential expression between lung cancer patient and normal person, it is thus achieved that difference expression gene；

3) single factor test COX model analysis step 2 is used) dependency between gained difference expression gene and life span, screening Go out the gene relevant to life span；

4) use multifactor COX model stepwise regression analysis to screen gained gene, obtain the prognostic predictive model of pulmonary carcinoma.

Preferably, for ensure result accuracy, described by imperfect for clinic Survival data and total life span be less than one After the patient data of individual month is deleted, then carry out step 2).

Below in conjunction with specific embodiment, the present invention is further illustrated, but is not limited thereto.

Embodiment 1

As a example by adenocarcinoma of lung, the foundation of the prognostic predictive model of adenocarcinoma of lung:

1) adenocarcinoma of lung data are downloaded from TCGA, including transcript profile data and clinical Survival data, totally 515 samples；

2) remove the incomplete sample of clinical data, as followed up a case by regular visits to incomplete data, remain 464 samples；, the present invention includes in The TTL of following up a case by regular visits to of patient is 1986 the earliest, is 2016 the latest；

3) use DESeq R bag compare gene expression amount between normal person and cancer patient (screening conditions are: | log2FC | > 2, padj < 0.001) 1618 difference expression genes, are obtained；The main code that DESeq searches differential gene is as follows:

>library("DESeq")

>group=c(rep("normal",num1),rep("tumor",num2))

>design = factor(group)

>newTab = newCountDataSet( data, design )

>newTab = estimateSizeFactors(newTab)

>newTab = estimateDispersions(newTab, fitType = "local")

>diff = nbinomTest(newTab, "normal", "tumor")

4) single factor test COX for analyzing the relation of life span and differential gene, find out the gene relevant to life span (p < 0.001) 131 genes relevant to existence, are obtained；Single factor test COX code analysis is as follows:

>library(survival)

>cox <- coxph(Surv(futime, fustat) ~ geneName, data = rt)

5) gene that the life span obtained for single factor analysis is relevant, we use multifactor COX to be analyzed, employing It it is method of gradual regression.Finally, we obtain the prognostic predictive model of the pulmonary carcinoma for assessing adenocarcinoma of lung life cycle；Multifactor COX Code analysis is as follows:

>library(survival)

>cox <- coxph(Surv(futime, fustat) ~ ., data = rt)

>cox=step(cox,direction = "both")

>coxSummary=summary(cox)

The prognostic predictive model of described pulmonary carcinoma is:

S=∑Pi * Ci

Wherein, S is lung cancer for prognosis value-at-risk, and Pi is the quantitative expression value of i-th bit gene, and Ci represents the COX of i-th bit gene and returns Return coefficient, i=1,2,3,4 ... n, n are the sum of the prognostic markers thing of pulmonary carcinoma.

6) COX is used to analyze the model that obtains to each sample calculation risk value, according to the median of value-at-risk by patient It is divided into excessive risk group and low-risk group.The product method of limits is used to estimate survival rate and use Kaplan-Meier (KM) method Estimate and draw survival curve, and use log-rank inspection to carry out the comparison of height risk group survival curve, through higher Both low-risk groups significant difference, during such that it is able to instruct clinician by the existence of the expression prediction patient of detection gene Between, it is provided that individualized treatment scheme.

Embodiment 2

The prognostic markers thing of adenocarcinoma of lung include the following assortment of genes: RP11-89K21.1, CDH17, GRIA1, CENPF, AP000438.2, HMMR, RP11-434D9.1, ADH1B, TPX2, OR7E47P, the sequence of described gene is followed successively by SEQ ID NO:1～10.

According to embodiment 1, we filter out above prognostic markers thing, and the prognostic predictive model of described pulmonary carcinoma is:

S=∑Pi * Ci

Wherein, S is lung cancer for prognosis value-at-risk, and Pi is the quantitative expression value of i-th bit gene, and Ci represents the COX of i-th bit gene and returns Return coefficient, i=1,2,3,4 ... 10.

In the prognostic predictive model of described pulmonary carcinoma, the COX regression coefficient that each gene pairs is answered is selected from following table:

The method for prediction of prognosis of a kind of pulmonary carcinoma, comprises the following steps:

1) gather sample from patients with lung cancer, detect and obtain the expression of the prognostic markers thing of pulmonary carcinoma；

2) expression obtained is applied to the prognostic predictive model of pulmonary carcinoma, to obtain lung cancer for prognosis value-at-risk；

3) obtained lung cancer for prognosis value-at-risk is compared with reference value 1.02, determines the prognosis of described patient:

If detection calculates patient's prognostic risk value less than or equal to 1.02, then this patient just belongs to low-risk, permissible Predict that this patient's five year survival rate is 56.4%；Whereas if patient's value-at-risk is more than 1.02, then this patient just belongs to high Risk, can predict that this patient's five year survival rate is 18.1%.

Using log-rank inspection that the present embodiment model carries out the comparison of height risk group survival curve, result is shown in Fig. 1, Abscissa represents the life span (in units of year) of patient, and vertical coordinate represents overall survival.Blue lines (above one) generation Table low-risk group, red lines (following) represent excessive risk group, and log-rank checks significance p=0, description standard model Height risk group between survival curve there is significant difference.Low-risk five year survival rate is 56.4%, excessive risk life in 5 years The rate of depositing is 18.1%, and five year survival rate difference is substantially.

Application ROC curve figure evaluates the clinical accuracy of the present embodiment model, and ROC curve figure is shown in that Fig. 2, abscissa are false sun Property rate (1-specificity), vertical coordinate is True Positive Rate (sensitivity), calculate master pattern AUC be 0.746, model prediction is described Result accuracy is higher.Area value under ROC curve is between 1.0 and 0.5, at AUC > in the case of 0.5, AUC is closer to 1, illustrate that diagnosis effect is the best.

Embodiment 3

The prognostic markers thing of adenocarcinoma of lung include the following assortment of genes: RP11-89K21.1, CDH17, GRIA1, CENPF, AP000438.2, HMMR, RP11-434D9.1, ADH1B, TPX2, OR7E47P, GPR37, HABP2, the sequence of described gene depends on Secondary for SEQ ID NO:1～12.

In the method for prediction of prognosis of pulmonary carcinoma, described reference value is 1.02.

According to embodiment 1, we filter out above prognostic markers thing, and the prognostic predictive model of described pulmonary carcinoma is:

S=∑Pi * Ci

Wherein, S is lung cancer for prognosis value-at-risk, and Pi is the quantitative expression value of i-th bit gene, and Ci represents the COX of i-th bit gene and returns Return coefficient, i=1,2,3,4 ... 12.

In the prognostic predictive model of described pulmonary carcinoma, the COX regression coefficient that each gene pairs is answered is selected from following table:

The method for prediction of prognosis of a kind of pulmonary carcinoma, comprises the following steps:

1) gather sample from patients with lung cancer, detect and obtain the expression of the prognostic markers thing of pulmonary carcinoma；

2) expression obtained is applied to the prognostic predictive model of pulmonary carcinoma, to obtain lung cancer for prognosis value-at-risk；

3) obtained lung cancer for prognosis value-at-risk is compared with reference value 0.986, determines the prognosis of described patient:

If detection calculates patient's prognostic risk value less than or equal to 0.986, then this patient just belongs to low-risk, permissible Predict that this patient's five year survival rate is 60.1%；Whereas if patient's value-at-risk is more than 0.986, then this patient just belongs to high Risk, can predict that this patient's five year survival rate is 14.5%.

Using log-rank inspection that the present embodiment model carries out the comparison of height risk group survival curve, result is shown in Fig. 3, Log-rank checks significance p=0, and the survival curve between the height risk group of description standard model has significant difference.Low Risk five year survival rate is 60.1%, and excessive risk five year survival rate is 14.5%, and five year survival rate difference is substantially.

Application ROC curve figure evaluates the clinical accuracy of the present embodiment model, and ROC curve figure is shown in that Fig. 4, abscissa are false sun Property rate (1-specificity), vertical coordinate is True Positive Rate (sensitivity), calculate master pattern AUC be 0.744, model prediction is described Result accuracy is higher.Area value under ROC curve is between 1.0 and 0.5, at AUC > in the case of 0.5, AUC is closer to 1, illustrate that diagnosis effect is the best.

Embodiment 4

The prognostic markers thing of adenocarcinoma of lung include the following assortment of genes: RP11-89K21.1, CDH17, GRIA1, CENPF, AP000438.2, HMMR, RP11-434D9.1, ADH1B, TPX2, OR7E47P, GPR37, HABP2, the sequence of described gene depends on Secondary for SEQ ID NO:1～12.

In the method for prediction of prognosis of pulmonary carcinoma, described reference value is 0.979.

According to embodiment 1, we filter out above prognostic markers thing, and the prognostic predictive model of described pulmonary carcinoma is:

S=∑Pi * Ci

Wherein, S is lung cancer for prognosis value-at-risk, and Pi is the quantitative expression value of i-th bit gene, and Ci represents the COX of i-th bit gene and returns Return coefficient, i=1,2,3,4 ... 34.

In the prognostic predictive model of described pulmonary carcinoma, the COX regression coefficient that each gene pairs is answered is selected from following table:

The method for prediction of prognosis of a kind of pulmonary carcinoma, comprises the following steps:

1) gather sample from patients with lung cancer, detect and obtain the expression of the prognostic markers thing of pulmonary carcinoma；

2) expression obtained is applied to the prognostic predictive model of pulmonary carcinoma, to obtain lung cancer for prognosis value-at-risk；

3) obtained lung cancer for prognosis value-at-risk is compared with reference value 0.979, determines the prognosis of described patient:

If detection calculates patient's prognostic risk value less than or equal to 0.979, then this patient just belongs to low-risk, permissible Predict that this patient's five year survival rate is 62.1%；Whereas if patient's value-at-risk is more than 0.979, then this patient just belongs to high Risk, can predict that this patient's five year survival rate is 11.3%.

Using log-rank inspection that master pattern carries out the comparison of height risk group survival curve, result is shown in Fig. 1, horizontal seat Mark represents the life span (in units of year) of patient, and vertical coordinate represents overall survival.Blue lines (above) represent low Risk group, red lines (following) represent excessive risk group, and log-rank checks significance p=0, the height of description standard model Survival curve between low-risk group has significant difference.Low-risk five year survival rate is 62.1% (52.3-73.8), Gao Feng Danger five year survival rate is 11.3% (5.55-22.8), and five year survival rate differs clearly.

Application ROC curve figure evaluates the clinical accuracy of the present embodiment model, and ROC curve figure is shown in that Fig. 6, abscissa are false sun Property rate (1-specificity), vertical coordinate is True Positive Rate (sensitivity), calculate master pattern AUC be 0.804, model prediction is described Result accuracy is higher.Area value under ROC curve is between 1.0 and 0.5, at AUC > in the case of 0.5, AUC is closer to 1, illustrate that diagnosis effect is the best.

The model that the present invention sets up is rna-seq and the clinical data downloading lung cancer patient from TCGA, solves sample and receives Collection difficulty, checks order costly, and the problem to patient follow-up.TCGA, i.e. cancer and oncogene collection of illustrative plates (Cancer Genome Atlas) plan, it is intended to by application genome analysis technology, especially with large-scale gene order-checking, the mankind are whole The genome mutation collection of illustrative plates of cancer is drawn out.Additionally, the follow up data of TCGA is also made the most perfect, can easily from TCGA downloads the up-to-date follow up data of patient.

Owing to the data of life cycle typically disobeys normal distribution, and the life span of patient has a lot of censored data, I Select Cox proportional hazards regression models as statistical model.It addition, we use the r increased income to be programmed, beneficially journey The automatization of sequence.

SEQUENCE LISTING

<110>Guangzhou ten thousand moral gene medical science and technology company limited

<120>the prognostic markers thing of a kind of pulmonary carcinoma, the method using this labelling prediction lung cancer for prognosis and application

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ccaatggcca gctttattac cagattgtca tccagcttcc catgatcaac aatgtcatgt 660

actttcagat caacaacaaa acgggagcca tctctcttac ccgagaggga tctcaggaat 720

tgaatcctgc taagaatcct tcctataatc tggtgatctc agtgaaggac atgggaggcc 780

agagtgagaa ttccttcagt gataccacat ctgtggatat catagtgaca gagaatattt 840

ggaaagcacc aaaacctgtg gagatggtgg aaaactcaac tgatcctcac cccatcaaaa 900

tcactcaggt gcggtggaat gatcccggtg cacaatattc cttagttgac aaagagaagc 960

tgccaagatt cccattttca attgaccagg aaggagatat ttacgtgact cagcccttgg 1020

accgagaaga aaaggatgca tatgtttttt atgcagttgc aaaggatgag tacggaaaac 1080

cactttcata tccgctggaa attcatgtaa aagttaaaga tattaatgat aatccaccta 1140

catgtccgtc accagtaacc gtatttgagg tccaggagaa tgaacgactg ggtaacagta 1200

tcgggaccct tactgcacat gacagggatg aagaaaatac tgccaacagt tttctaaact 1260

acaggattgt ggagcaaact cccaaacttc ccatggatgg actcttccta atccaaacct 1320

atgctggaat gttacagtta gctaaacagt ccttgaagaa gcaagatact cctcagtaca 1380

acttaacgat agaggtgtct gacaaagatt tcaagaccct ttgttttgtg caaatcaacg 1440

ttattgatat caatgatcag atccccatct ttgaaaaatc agattatgga aacctgactc 1500

ttgctgaaga cacaaacatt gggtccacca tcttaaccat ccaggccact gatgctgatg 1560

agccatttac tgggagttct aaaattctgt atcatatcat aaagggagac agtgagggac 1620

gcctgggggt tgacacagat ccccatacca acaccggata tgtcataatt aaaaagcctc 1680

ttgattttga aacagcagct gtttccaaca ttgtgttcaa agcagaaaat cctgagcctc 1740

tagtgtttgg tgtgaagtac aatgcaagtt cttttgccaa gttcacgctt attgtgacag 1800

atgtgaatga agcacctcaa ttttcccaac acgtattcca agcgaaagtc agtgaggatg 1860

tagctatagg cactaaagtg ggcaatgtga ctgccaagga tccagaaggt ctggacataa 1920

gctattcact gaggggagac acaagaggtt ggcttaaaat tgaccacgtg actggtgaga 1980

tctttagtgt ggctccattg gacagagaag ccggaagtcc atatcgggta caagtggtgg 2040

ccacagaagt aggggggtct tccttgagct ctgtgtcaga gttccacctg atccttatgg 2100

atgtgaatga caaccctccc aggctagcca aggactacac gggcttgttc ttctgccatc 2160

ccctcagtgc acctggaagt ctcattttcg aggctactga tgatgatcag cacttatttc 2220

ggggtcccca ttttacattt tccctcggca gtggaagctt acaaaacgac tgggaagttt 2280

ccaaaatcaa tggtactcat gcccgactgt ctaccaggca cacagagttt gaggagaggg 2340

agtatgtcgt cttgatccgc atcaatgatg ggggtcggcc acccttggaa ggcattgttt 2400

ctttaccagt tacattctgc agttgtgtgg aaggaagttg tttccggcca gcaggtcacc 2460

agactgggat acccactgtg ggcatggcag ttggtatact gctgaccacc cttctggtga 2520

ttggtataat tttagcagtt gtgtttatcc gcataaagaa ggataaaggc aaagataatg 2580

ttgaaagtgc tcaagcatct gaagtcaaac ctctgagaag ctgaatttga aaaggaatgt 2640

ttgaatttat atagcaagtg ctatttcagc aacaaccatc tcatcctatt acttttcatc 2700

taacgtgcat tataattttt taaacagata ttccctcttg tcctttaata tttgctaaat 2760

atttcttttt tgaggtggag tcttgctctg tcgcccaggc tggagtacag tggtgtgatc 2820

ccagctcact gcaacctccg cctcctgggt tcacatgatt ctcctgcctc agcttcctaa 2880

gtagctgggt ttacaggcac ccaccaccat gcccagctaa tttttgtatt tttaatagag 2940

acggggtttc gccatttggc caggctggtc ttgaactcct gacgtcaagt gatctgcctg 3000

ccttggtctc ccaatacagg catgaaccac tgcacccacc tacttagata tttcatgtgc 3060

tatagacatt agagagattt ttcatttttc catgacattt ttcctctctg caaatggctt 3120

agctacttgt gtttttccct tttggggcaa gacagactca ttaaatattc tgtacatttt 3180

ttctttatca aggagatata tcagtgttgt ctcatagaac tgcctggatt ccatttatgt 3240

tttttctgat tccatcctgt gtccccttca tccttgactc ctttggtatt tcactgaatt 3300

tcaaacattt gtcagagaag aaaaacgtga ggactcagga aaaataaata aataaaagaa 3360

cagccttttc ccttagtatt aacagaaatg tttctgtgtc attaaccatc tttaatcaat 3420

gtgacatgtt gctctttggc tgaaattctt caacttggaa atgacacaga cccacagaag 3480

gtgttcaaac acaacctact ctgcaaacct tggtaaagga accagtcagc tggccagatt 3540

tcctcactac ctgccatgca tacatgctgc gcatgttttc ttcattcgta tgttagtaaa 3600

gttttggtta ttatatattt aacatgtgga agaaaacaag acatgaaaag agtggtgaca 3660

aatcaagaat aaacactggt tgtagtcagt ttt 3693

<210> 3

<211> 5708

<212> DNA

<213>mankind GRIA1 gene

<400> 3

gtgtgagggg gagagcgaga gagagcaagg gagggagaga gaggcaggct gcgaggggag 60

aggagaggga gtgggggagc cagcgctcca gctagcatga ggacgggctt cttttcccgt 120

gctcagttaa tctggctgtc agttggtgtt aacgctgcag tttaagtgtt cggattccaa 180

gggaaacaga caaacctcac gaaaggaagg aagcaagcaa gcaaggaagg aactgcagga 240

ggaaaagaac aggcagaaca gcgagaagaa taaagggaaa gggggggaaa caccaaatct 300

atgattggac ctgggcttct ttttcgccaa tgcaaaaagg aatatgcagc acatttttgc 360

cttcttctgc accggtttcc taggcgcggt agtaggtgcc aatttcccca acaatatcca 420

gatcggggga ttatttccaa accagcagtc acaggaacat gctgctttta gatttgcttt 480

gtcgcaactc acagagcccc cgaagctgct cccccagatt gatattgtga acatcagcga 540

cagctttgag atgacctata gattctgttc ccagttctcc aaaggagtct atgccatctt 600

tgggttttat gaacgtagga ctgtcaacat gctgacctcc ttttgtgggg ccctccacgt 660

ctgcttcatt acgccgagct ttcccgttga tacatccaat cagtttgtcc ttcagctgcg 720

ccctgaactg caggatgccc tcatcagcat cattgaccat tacaagtggc agaaatttgt 780

ctacatttat gatgccgacc ggggcttatc cgtcctgcag aaagtcctgg atacagctgc 840

tgagaagaac tggcaggtga cagcagtcaa cattttgaca accacagagg agggataccg 900

gatgctcttt caggacctgg agaagaaaaa ggagcggctg gtggtggtgg actgtgaatc 960

agaacgcctc aatgctatct tgggccagat tataaagcta gagaagaatg gcatcggcta 1020

ccactacatt cttgcaaatc tgggcttcat ggacattgac ttaaacaaat tcaaggagag 1080

tggcgccaat gtgacaggtt tccagctggt gaactacaca gacactattc cggccaagat 1140

catgcagcag tggaagaata gtgatgctcg agaccacaca cgggtggact ggaagagacc 1200

caagtacacc tctgcgctca cctacgatgg ggtgaaggtg atggctgagg ctttccagag 1260

cctgcggagg cagagaattg atatatctcg ccgggggaat gctggggatt gtctggctaa 1320

cccagctgtt ccctggggcc aagggatcga catccagaga gctctgcagc aggtgcgatt 1380

tgaaggttta acaggaaacg tgcagtttaa tgagaaagga cgccggacca actacacgct 1440

ccacgtgatt gaaatgaaac atgacggcat ccgaaagatt ggttactgga atgaagatga 1500

taagtttgtc cctgcagcca ccgatgccca agctgggggc gataattcaa gtgttcagaa 1560

cagaacatac atcgtcacaa caatcctaga agatccttat gtgatgctca agaagaacgc 1620

caatcagttt gagggcaatg accgttacga gggctactgt gtagagctgg cggcagagat 1680

tgccaagcac gtgggctact cctaccgtct ggagattgtc agtgatggaa aatacggagc 1740

ccgagaccct gacacgaagg cctggaatgg catggtggga gagctggtct atggaagagc 1800

agatgtggct gtggctccct taactatcac tttggtccgg gaagaagtta tagatttctc 1860

caaaccattt atgagtttgg ggatctccat catgattaaa aaaccacaga aatccaagcc 1920

gggtgtcttc tccttccttg atcctttggc ttatgagatt tggatgtgca ttgtttttgc 1980

ctacattgga gtgagtgttg tcctcttcct ggtcagccgc ttcagtccct atgaatggca 2040

cagtgaagag tttgaggaag gacgggacca gacaaccagt gaccagtcca atgagtttgg 2100

gatattcaac agtttgtggt tctccctggg agccttcatg cagcaaggat gtgacatttc 2160

tcccaggtcc ctgtctggtc gcatcgttgg tggcgtctgg tggttcttca ccttaatcat 2220

catctcctca tatacagcca atctggccgc cttcctgacc gtggagagga tggtgtctcc 2280

cattgagagt gcagaggacc tagcgaagca gacagaaatt gcctacggga cgctggaagc 2340

aggatctact aaggagttct tcaggaggtc taaaattgct gtgtttgaga agatgtggac 2400

atacatgaag tcagcagagc catcagtttt tgtgcggacc acagaggagg ggatgattcg 2460

agtgaggaaa tccaaaggca aatatgccta cctcctggag tccaccatga atgagtacat 2520

tgagcagcgg aaaccctgtg acaccatgaa ggtgggaggt aacttggatt ccaaaggcta 2580

tggcattgca acacccaagg ggtctgccct gagaaatcca gtaaacctgg cagtgttaaa 2640

actgaacgag caggggcttt tggacaaatt gaaaaacaaa tggtggtacg acaagggcga 2700

gtgcggcagc gggggaggtg attccaagga caagacaagc gctctgagcc tcagcaatgt 2760

ggcaggcgtg ttctacatcc tgatcggagg acttggacta gccatgctgg ttgccttaat 2820

cgagttctgc tacaaatccc gtagtgaatc caagcggatg aagggttttt gtttgatccc 2880

acagcaatcc atcaacgaag ccatacggac atcgaccctc ccccgcaaca gcggggcagg 2940

agccagcagc ggcggcagtg gagagaatgg tcgggtggtc agccatgact tccccaagtc 3000

catgcaatcg attccttgca tgagccacag ttcagggatg cccttgggag ccacgggatt 3060

gtaactggag cagatggaga ccccttgggg agcaggctcg ggctccccag ccccatccca 3120

aacccttcag tgccaaaaac aacaacaaaa tgaaacgcaa ccaccaccaa ccactgcgac 3180

cacaagaagg atgattcaac aggttttcct gaagaattga aaaaccattt tgctgtccct 3240

tttccttttt tgatgttctt tcaccctttt ctgtttgcta agtgaggatg aaaaaataac 3300

actgtactgc aataagggga gagtaaccct gtctaatgaa acctgtgtct ctgagagtag 3360

agtcactgga acactaatga ggaaactgca ctgttttatt ttaattcagt tgttagtgtg 3420

tcttagtgtg tgcaattttt tttcttacta atatccatgg tttgcaggtt ctgttaggcc 3480

ctttccttct ccttacttct tatccccaac tccctaccca cccctcttca gttttcagat 3540

tggagattca agatttgttc cactttacaa gcaagaggaa aaaaaagcaa ccttcaaact 3600

aattctccat gggggctctc catgttaccc tccactcctt ggcccaaacc tctgatggag 3660

atagacattg ttggagaagt gggctgcctt ccccaagtgg ggcactgctt aagcacttat 3720

tcagtggaga acacaggtga aaagcaactc aggatgaggg tggtggagag ggcaggggca 3780

gatgtgcagt cagagaagga ctcctgaagt tactgctgct cagaaaaaca gttcctttaa 3840

tgtggaagag ccatttcata ggtcataggt ggtatggtat atttcttcag agtcaacctt 3900

ggccctgaga agtatgtcct cctggtgtgc tcaggctcaa cggcagtctg gtggctgaag 3960

gcacttggcc tcctaaacca agcagaattt tgggaagaga taacagccag ggagatattg 4020

cccatgattc tcactttttc tttgcctggc atctaagcag gaacccattg tggagtagac 4080

tctcttcttc tatggagcct ctgacatggg gagcaatgct aagcaagcta agtgtaaaag 4140

aaaagtgaca gaataatttt ggaagaggaa gcctcatcaa aagctcacac aaaatagagc 4200

ttcccatggt gtgccctatc ctaggtttaa gaaaacacgt atgaagttta tgctgatgca 4260

aagaacttgg gtttttatgt taatataaag tgttgtttta gcatgtggcc agatgatgct 4320

ctgtcatctt tagaaagtga gataaccaag gaaataattg aaggagtata gggagatgga 4380

ttaagttgat aatgacattt agggcaactt aagacctttg atcccaggtt ctaactcaaa 4440

gaggctgacc ttcccccagc taagatagca tgaggacgtt gtattccaat atacgtatga 4500

ttggggctac aaagctgaac taaagcaaga ttggtgaagt ggcagggttt atagagagaa 4560

gcccaggctg agttcagctt ttgttggaag tgagaatccc tgacatatag ctttcttgga 4620

gatcccaact ctcattcttg gtgcaactgg cttccagctc tccagcagtc actctcctag 4680

gtgcatgatt cagtgcgtgc catgtgtcat tagcttttac tgataaccat attctggctt 4740

gttcccttac cccctacttc tatccaattt tctctgctag gggttatcat tagcaattga 4800

catgctaaag gttttggagc ccacctaggg gtaggtgcag ctttattggc ttttctgtgg 4860

attctctcag tggacccaca ccatctctat gtctctccac tctcctgcct tcagccatag 4920

caaagaatcc ttccaaaatc aaactcttca cttttttgac tcaagtgttg ttgttcagtc 4980

tctcgcgtgt caatgtggtc atggttcatg aaaccggacc ctcaagatgg atgattgctt 5040

ttaactactg ccagctgatg tctctcagcc cctgccctca tacaagattt ttctcagcct 5100

tcagcctacc actgcagaat ccgatgtgac ccaccattag ggagtctgca tcttggaaga 5160

gttggaaata accctttaac atcaacatgc ttcaaagact ttttgccttt ggcctagtaa 5220

gatgcctctc cagctactga gcccacaagt aacatgagcg gataaaaaga gacttgtttg 5280

tgctagaaat gagggtctat gctatgaggg ggtccaagac tctggcgaaa tgtgcttttt 5340

catcaatgga gaaatgaaag gaaaacacaa gcaagaaaaa agttaacttg tattatgtat 5400

ttttactaca cttttcttaa aaatagagca ttgggaaaac tctgaaagag actgacattt 5460

ttctcaacag gaatccatac ttaacagttc tggctttcat taaattttgc tctttggtac 5520

ctgggccttt tatttaacat ctatatttgt tttaactctc ttggcagatg tgtgaaagga 5580

ttcttgcttg atcaaacact aagtattttt ttggttcttg tttttctttc aaatagccag 5640

gtttttttct tttggtattt gcataaaatg aaaatatcac cgaatattaa atcactgtgg 5700

atccatta 5708

<210> 4

<211> 689

<212> DNA

<213>mankind CENPF gene

<400> 4

agaagcgggc gaattgggca ccggtggcgg ctgcgggcag tttgaattag actctgggct 60

ccagcccgcc gaagccgcgc cagaactgta ctctccgaga ggtcgttttc ccgtccccga 120

gagcaagttt atttacaaat gttggagtaa taaagaaggc agaacaaaat gagctgggct 180

ttggaagaat ggaaagaagg gctgcctaca agagctcttc agaaaattca agagcttgaa 240

ggacagcttg acaaactgaa gaaggaaaag cagcaaaggc agtttcagct tgacagtctc 300

gaggctgcgc tgcagaagca aaaacagaag gttgaaaatg aaaaaaccga gggtacaaac 360

ctgaaaaggg agaatcaaag attgatggaa atatgtgaaa gtctggagaa aactaagcag 420

aagatttctc atgaacttca agtcaaggag tcacaagtga atttccagga aggacaactg 480

aattcaggca aaaaacaaat agaaaaactg gaacaggaac ttaaaaggta atattttggg 540

atggtattta tagggatgat atttgtatgt attaatcaga tgcgtttgtc ttttccttta 600

acaatataat tattatactt tgcaattttt tttcctggta gaataagtaa tgattcggtc 660

tctgtaccgt aacttgaaag agtggaaga 689

<210> 5

<211> 749

<212> DNA

<213>mankind AP000438.2 gene

<400> 5

cgaagtctgg aaagttggca gggtctctct gggctgccca ggaaggtgac ccagggtcct 60

gggaagccag gtactgccag aagaggatgg ctggggccca gagcacaaag gaaaccagcc 120

aggccaggcc gatcatcaga gctgcccggc ggggtgtgcg cttggcacgg tagctcaggg 180

gccgagtcac ggagaagtag cggtcaaagc tgatgagcag cagattcatg acggaggcat 240

tgctggccac atagtccagg gccagccaga ggtcacaagc cagcgtgccc agagcccagt 300

ggcccatgag caggtcctgc tatccccagt ttgcaaaagg cctaacctga ggcttcaaga 360

cctgaaggac cttacagctc atcagcgatg caaccaggat ttggatccag gccgtctgcc 420

tccgagacca acctcttgaa ccctactcct gcccttgagg ggttgcgggg gtttgccagc 480

ctccttgccc acctcagcta gcttactcaa gcccaacttt gaatcagtta acgatgagca 540

atcggtcctt ctttcagaat ctggcctcct ttgtgtgcgc ggcagtgtct gtcgtatatc 600

tgtgtgatgt gtgataacag tgctgtggga agagggtggg tctactggag ctctttaaag 660

ggcagggtaa gggctgcgtc tgtctcattc aatgctttat cccagggctt ggcagatagt 720

agttgctcaa taaatacctg tggaataaa 749

<210> 6

<211> 720

<212> DNA

<213>mankind HMMR gene

<400> 6

tttgaatggc cgtttacggc accggcaggc cccggatgaa agaactgaaa tccagaaaag 60

ttcacatgca acgaacaatg ggacagaatt ggaaccagtg aagccggaac tcactacacc 120

gtcccctaag tgacgccaaa atgcactttc taacgtctcc ggaggttgtg caccatctcc 180

aggtgcttat gatgttaaaa ctttagaagt attgaaagga ccagtatcct ttcagaaatc 240

acaaagattt aaacaacaaa aagaatctaa acaaaatctt aatgttgaca aagatactac 300

cttgcctgct tcagctagaa aagttaagtc ttcggaatca aagattcgtg ttcttctaca 360

ggaacgtggt gcccaggaca ggcggatcca ggatctggaa actgagttgg aaaagatgga 420

agcaaggcta aatgctgcac taagggaaaa aacatctctc tctgcaaata atgctacact 480

ggaaaaacaa cttattgaat tgaccaggac taatgaacta ctaaaatcta agttttctga 540

aaatggtaac cagaagaatt tgagaattct aagcttggag ttgatgaaac ttagaaacaa 600

aagagaaaca aagatgaggg gtatgatggc taagcaagaa ggcatggaga tgaagctgca 660

ggtcacccaa aggagtctcg aagagtctca agggaaaata gcccaactgg agggaaaact 720

<210> 7

<211> 780

<212> DNA

<213>mankind RP11-434D9.1 gene

<400> 7

cccagtgtga agtgaaagca aagttttaaa attttttctc ctctgcaaga aaatatattt 60

cagaaaatct ctatctcaaa aatgaagcaa ttaagaactg tctggctgtg aggatctggc 120

ctcaacttga cttaaagcaa caacttcagt tacatatcaa gatgtgcgtg agaatgaacc 180

tgctctgctc agccaacgtg ctgagtgact tgatgtttgt aataaataaa taaataaaca 240

acaaacaggt tgtcatgaaa tatcctttgt aattataaac acaggaaact ttctgagatg 300

caagtgggtg ttaaatggtt cagcaaagcc aagccagagc agttggtgtg ctttgaaatg 360

aagcaacatt ggaaaaatga aatctactga aagatcacta cgtgtttcaa agaactgact 420

agaaccagaa ctcttcagaa aagaatgtgc taaaggtttg tccttgagaa gtttccaagc 480

caagacactc ctaggagcac ttcccattga ggccgaaagg ctcatccact tacagcgatt 540

catgggaaaa gaggacagca ccctcatctt tggtccaaga acacaatcct gagtgacaca 600

ggattgccct gaaagtcaac agcatccgtc ttacaaaaat caacaacact cgcttttcaa 660

acttccaaag aatttactgg tctggtttgg ccttttggtg ccacattcca ttccagaacc 720

ccagctaaaa tgttctcaag ggctgaccag agattcctcc tcctttctct gggctggtac 780

<210> 8

<211> 4072

<212> DNA

<213>mankind ADH1B gene

<400> 8

gcactcaagc agagaagaaa tccacaaaga ctcacagtct gctggtgggc agagaagaca 60

gaaacgacat gagcacagca ggaaaagtaa tcaaatgcaa agcagctgtg ctatgggagg 120

taaagaaacc cttttccatt gaggatgtgg aggttgcacc tcctaaggct tatgaagttc 180

gcattaagat ggtggctgta ggaatctgtc acacagatga ccacgtggtt agtggcaacc 240

tggtgacccc ccttcctgtg attttaggcc atgaggcagc cggcatcgtg gagagtgttg 300

gagaaggggt gactacagtc aaaccaggtg ataaagtcat cccgctcttt actcctcagt 360

gtggaaaatg cagagtttgt aaaaacccgg agagcaacta ctgcttgaaa aatgatctag 420

gcaatcctcg ggggaccctg caggatggca ccaggaggtt cacctgcagg gggaagccca 480

ttcaccactt ccttggcacc agcaccttct cccagtacac ggtggtggat gagaatgcag 540

tggccaaaat tgatgcagcc tcgcccctgg agaaagtctg cctcattggc tgtggattct 600

cgactggtta tgggtctgca gttaacgttg ccaaggtcac cccaggctct acctgtgctg 660

tgtttggcct gggaggggtc ggcctatctg ctgttatggg ctgtaaagca gctggagcag 720

ccagaatcat tgcggtggac atcaacaagg acaaatttgc aaaggccaaa gagttgggtg 780

ccactgaatg catcaaccct caagactaca agaaacccat tcaggaagtg ctaaaggaaa 840

tgactgatgg aggtgtggat ttttcgtttg aagtcatcgg tcggcttgac accatgatgg 900

cttccctgtt atgttgtcat gaggcatgtg gcacaagcgt catcgtaggg gtacctcctg 960

cttcccagaa cctctcaata aaccctatgc tgctactgac tggacgcacc tggaaggggg 1020

ctgtttatgg tggctttaag agtaaagaag gtatcccaaa acttgtggct gattttatgg 1080

ctaagaagtt ttcactggat gcgttaataa cccatgtttt accttttgaa aaaataaatg 1140

aaggatttga cctgcttcac tctgggaaaa gtatccgtac cgtcctgacg ttttgaggca 1200

atagagatgc cttcccctgt agcagtcttc agcctcctct accctacaag atctggagca 1260

acagctagga aatatcatta attcagctct tcagagatgt tatcaataaa ttacacatgg 1320

gggctttcca aagaaatgga aattgatggg aaattatttt tcaggaaaat ttaaaattca 1380

agtgagaagt aaataaagtg ttgaacatca gctggggaat tgaagccaac aaaccttcct 1440

tcttaaccat tctactgtgt cacctttgcc attgaggaaa aatattcctg tgacttcttg 1500

catttttggt atcttcataa tctttagtca tcgaatccca gtggagggga cccttttact 1560

tgccctgaac atacacatgc tgggccattg tgattgaagt cttctaactc tgtctcagtt 1620

ttcactgtcg acattttcct ttttctaata aaaatgtacc aaatccctgg ggtaaaagct 1680

agggtaaggt aaaggataga ctcacattta caagtagtga aggtccaaga gttctaaata 1740

caggaaattt cttaggaact caaataaaat gccccacatt ttactacagt aaatggcagt 1800

gtttttatga cttttatact atttctttat ggtcgatata caattgattt tttaaaataa 1860

tagcagattt cttgcttcat atgacaaagc ctcaattact aattgtaaaa actgaactat 1920

tcccagaatc atgttcaaaa aatctgtaat ttttgctgat gaaagtgctt cattgactaa 1980

acagtattag tttgtggcta taaatgatta tttagatgat gactgaaaat gtgtataaag 2040

taattaaaag taatatggtg gctttaagtg tagagatggg atggcaaatg ctgtgaatgc 2100

agaatgtaaa attggtaact aagaaatggc acaaacacct taagcaatat attttcctag 2160

tagatatata tatacacata catatataca catatacaaa tgtatatttt tgcaaaattg 2220

ttttcaatct agaacttttc tattaactac catgtcttaa aatcaagtct ataatcctag 2280

cattagttta atattttgaa tatgtaaaga cctgtgttaa tgctttgtta atgcttttcc 2340

cactctcatt tgttaatgct ttcccactct caggggaagg atttgcattt tgagctttat 2400

ctctaaatgt gacatgcaaa gattattcct ggtaaaggag gtagctgtct ccaaaaatgc 2460

tattgttgca atatctacat tctatttcat attatgaaag accttagaca taaagtaaaa 2520

tagtttatca tttactgtgt gatcttcagt aagtctctca ggctctctga gcttgttcat 2580

cctttgtttt gaaaaaatta ctcaaccaat ccattacagc ttaaccaaga ttaaatggga 2640

tgatgttaat gaaagagctt cgccaattaa gaagggccat gtaaatgtgg gtaacctgat 2700

tgttttgtca aaaatgctaa agcggggtgg ggggtggcgc agtggcttat ccctgtaatc 2760

ccagcacttt gggaggtcaa gacgggtgga tgacctgagg tcaggagttt gagaccagcc 2820

tggtcaacat ggtgaaaccc catctctact aaaaatacaa aaatttgcca ggcatggtgg 2880

caggtggctg taatcccagc tactcaggag gctgaggcaa gagaatcgct ggagcctggg 2940

aggcggaggt tgcagtgagc agaaatcttg ccactgcaca gcagcctagg cgacaagagc 3000

aaaactctgt caaaagaaaa gaaaagaaaa tgctgaagga gttactcagg gtcaagaaag 3060

ttttcagaat gtccgtaaga taacatcatc atgggacact acctgttgct gggtgctctg 3120

cggcttttac actgatgctg attgtgtgag atccagaaaa agaaaatgga gttctacatg 3180

cccatctcac agcagggtca tcaaatcctg ctagtgacac agaagctgac atccagctcc 3240

cacccgcctt cctcccaggt tcggggagca ggtagagtgg gagccattag gtgggctctg 3300

ccagtgttgt catagagtgg caagtcctcc atgggatggg agtggcatgt gggagccttc 3360

tgcatcatgc actatgaaaa aagaaaattt actatggggc aagtggttca tatagttaag 3420

tgattgtata tatatatata tatatatata tatatatata tatatatata tatacactat 3480

ggggcaagtg gttcatatag ttaagtgatt gtatatatat atatatatat atatgtattc 3540

cagtgtagaa agtattggct gggtctgtaa tcaagtattc cacagaagtg ccttcatagg 3600

tgctgaaaca ggattatgag agtgacagac acaggactga agacagggag aaaatgtgca 3660

tggctgtgag aatatattgc tttgattctt tgacttctca tgatatatga aaacaaataa 3720

agtggggggg ggtgacttca ataatatcac gaacatattt aaaatgaaat tctgagcatg 3780

gagaaaaatt tcaaattctg gtataaacaa ataatctaaa aatatagata aggaaaaaat 3840

gttttcattt ttttggtcat attgatttgg gtcatatatg attgaccaaa aaaaaagata 3900

tttctaaaat cctggtgctt tttcatttaa aaatagacaa ccaaagagga aaatgaacaa 3960

tgatggaaat tcgtggaaga ggaaactaaa atggcaaaat agcatattaa atggcactga 4020

gccttggtgt gatcaaaaaa aaaaataaag caaaatgaga tattactgct ta 4072

<210> 9

<211> 3497

<212> DNA

<213>mankind TPX2 gene

<400> 9

tcaaaatttc aacgatactg aatgagtccc gcggcgggtt ggctcgcgct tcgttgtcag 60

atctgaggcg aggctaggtg agccgtggga agaaaagagg gagcagctag ggcgcgggtc 120

tccctcctcc cggagtttgg aacggctgaa gttcaccttc cagcccctag cgccgttcgc 180

gccgctaggc ctggcttctg aggcggttgc ggtgctcggt cgccgcctag gcggggcagg 240

gtgcgagcag gggcttcggg ccacgcttct cttggcgaca ggattttgct gtgaagtccg 300

tccgggaaac ggaggaaaaa aagagttgcg ggaggctgtc ggctaataac ggttcttgat 360

acatatttgc cagacttcaa gatttcagaa aaggggtgaa agagaagatt gcaactttga 420

gtcagacctg taggcctgat agactgatta aaccacagaa ggtgacctgc tgagaaaagt 480

ggtacaaata ctgggaaaaa cctgctcttc tgcgttaagt gggagacaat gtcacaagtt 540

aaaagctctt attcctatga tgccccctcg gatttcatca atttttcatc cttggatgat 600

gaaggagata ctcaaaacat agattcatgg tttgaggaga aggccaattt ggagaataag 660

ttactgggga agaatggaac tggagggctt tttcagggca aaactccttt gagaaaggct 720

aatcttcagc aagctattgt cacacctttg aaaccagttg acaacactta ctacaaagag 780

gcagaaaaag aaaatcttgt ggaacaatcc attccgtcaa atgcttgttc ttccctggaa 840

gttgaggcag ccatatcaag aaaaactcca gcccagcctc agagaagatc tcttaggctt 900

tctgctcaga aggatttgga acagaaagaa aagcatcatg taaaaatgaa agccaagaga 960

tgtgccactc ctgtaatcat cgatgaaatt ctaccctcta agaaaatgaa agtttctaac 1020

aacaaaaaga agccagagga agaaggcagt gctcatcaag atactgctga aaagaatgca 1080

tcttccccag agaaagccaa gggtagacat actgtgcctt gtatgccacc tgcaaagcag 1140

aagtttctaa aaagtactga ggagcaagag ctggagaaga gtatgaaaat gcagcaagag 1200

gtggtggaga tgcggaaaaa gaatgaagaa ttcaagaaac ttgctctggc tggaataggg 1260

caacctgtga agaaatcagt gagccaggtc accaaatcag ttgacttcca cttccgcaca 1320

gatgagcgaa tcaaacaaca tcctaagaac caggaggaat ataaggaagt gaactttaca 1380

tctgaactac gaaagcatcc ttcatctcct gcccgagtga ctaagggatg taccattgtt 1440

aagcctttca acctgtccca aggaaagaaa agaacatttg atgaaacagt ttctacatat 1500

gtgccccttg cacagcaagt tgaagacttc cataaacgaa cccctaacag atatcatttg 1560

aggagcaaga aggatgatat taacctgtta ccctccaaat cttctgtgac caagatttgc 1620

agagacccac agactcctgt actgcaaacc aaacaccgtg cacgggctgt gacctgcaaa 1680

agtacagcag agctggaggc tgaggagctc gagaaattgc aacaatacaa attcaaagca 1740

cgtgaacttg atcccagaat acttgaaggt gggcccatct tgcccaagaa accacctgtg 1800

aaaccaccca ccgagcctat tggctttgat ttggaaattg agaaaagaat ccaggagcga 1860

gaatcaaaga agaaaacaga ggatgaacac tttgaatttc attccagacc ttgccctact 1920

aagattttgg aagatgttgt gggtgttcct gaaaagaagg tacttccaat caccgtcccc 1980

aagtcaccag cctttgcatt gaagaacaga attcgaatgc ccaccaaaga agatgaggaa 2040

gaggacgaac cggtagtgat aaaagctcaa cctgtgccac attatggggt gccttttaag 2100

ccccaaatcc cagaggcaag aactgtggaa atatgccctt tctcgtttga ttctcgagac 2160

aaagaacgtc agttacagaa ggagaagaaa ataaaagaac tgcagaaagg ggaggtgccc 2220

aagttcaagg cacttccctt gcctcatttt gacaccatta acctgccaga gaagaaggta 2280

aagaatgtga cccagattga acctttctgc ttggagactg acagaagagg tgctctgaag 2340

gcacagactt ggaagcacca gctggaagaa gaactgagac agcagaaaga agcagcttgt 2400

ttcaaggctc gtccaaacac cgtcatctct caggagccct ttgttcccaa gaaagagaag 2460

aaatcagttg ctgagggcct ttctggttct ctagttcagg aaccttttca gctggctact 2520

gagaagagag ccaaagagcg gcaggagctg gagaagagaa tggctgaggt agaagcccag 2580

aaagcccagc agttggagga ggccagacta caggaggaag agcagaaaaa agaggagctg 2640

gccaggctac ggagagaact ggtgcataag gcaaatccaa tacgcaagta ccagggtctg 2700

gagataaagt caagtgacca gcctctgact gtgcctgtat ctcccaaatt ctccactcga 2760

ttccactgct aaactcagct gtgagctgcg gataccgccc ggcaatggga cctgctctta 2820

acctcaaacc taggaccgtc ttgctttgtc attgggcatg gagagaaccc atttctccag 2880

acttttacct acccgtgcct gagaaagcat acttgacaac tgtggactcc agttttgttg 2940

agaattgttt tcttacatta ctaaggctaa taatgagatg taactcatga atgtctcgat 3000

tagactccat gtagttactt cctttaaacc atcagccggc cttttatatg ggtcttcact 3060

ctgactagaa tttagtctct gtgtcagcac agtgtaatct ctattgctat tgccccttac 3120

gactctcacc ctctccccac tttttttaaa aattttaacc agaaaataaa gatagttaaa 3180

tcctaagata gagattaagt catggtttaa atgaggaaca atcagtaaat cagattctgt 3240

cctcttctct gcataccgtg aatttatagt taaggatccc tttgctgtga gggtagaaaa 3300

cctcaccaac tgcaccagtg aggaagaaga ctgcgtggat tcatggggag cctcacagca 3360

gccacgcagc aggctctggg tggggctgcc gttaaggcac gttctttcct tactggtgct 3420

gataacaaca gggaaccgtg cagtgtgcat tttaagacct ggcctggaat aaatacgttt 3480

tgtctttccc tccctga 3497

<210> 10

<211> 1422

<212> DNA

<213>mankind OR7E47P gene

<400> 10

ccgacgcagc cgccggcccg cccgccagtc tgggctcctg cacatctggc gatcccgcca 60

catctgggca gccggcgctg gagcatgaac ggctctcagg cgggcgccgc ggctcaggcc 120

gcctggctga gctcctgctg taaccagtcg gcgtcgccgc cggagccccc cgaggggccg 180

cgcgcggtgc aggcggtggt gctcggcgtg ctgtccctgc tggtgctttg cggggtcctg 240

ttcctgggcg gcggcctcct cctccgcgcc cagggcctga cagcgctgct gacccgcgag 300

cagcgcgcgt cccgcgagcc cgagccgggc agtgccagcg gagaggacgg cgacgacgac 360

tcctaggcgc ccggctgcgc tcggtggtcg cggcctccag gcagcccctg actccgagcg 420

gtccggagca tgcccgacgg ctgctgcggt cccgacccct tacccgaagc ggcgcgcccc 480

acacagagag gagaagaaga gaagaggaga ggagagaaga gaagaagaga ggagagaaga 540

gaagaggaga ggagagaaga gaacctcaga ggatccagaa cggcagctgg tccttgctgg 600

actgttcctg tccatgtgcc tggtcatggt gctggggaac ctgctcatca tccggccatg 660

agccctgact cccacctcca cacctccatg tacttcttcc tctccaacct gtccttgcct 720

gacatcggtt tcacctccac cacggtcccc cagatgactg tggacatcca gtctcgcagc 780

agagtcatct cctatgcagg ctgcctgact cagaagtctc tctttgccat ttttggaggc 840

acggaagaga gacatgctcc tgagtgtgat ggcctatgac cggtttgtag ccatctgtca 900

ccctctatat cattcagcca tcatgaacct gtgtttctgt ggcttcctag ttttgctgtc 960

tttttttttt ctcagtcttt tagactccca gctgtacaac ttgattgcct tactaatgac 1020

ctgcttcaag gaggtggaca ttcctaattt cttctgtgac ctttctcaac tcccccatct 1080

tgccgttgtg acaccttcat caataacata atcatgtatt tccctactgc catatttggt 1140

tttcttccca tctcggggac ccttttctct tactataaaa ttgtttcctc cattctgagg 1200

gtttcatcat caggtgggaa gtataaagcc ttctccacct gtgggtctca cctgtcagtt 1260

gtttgctgat tttatggaag aggtgttgga gggtacctca gttcagatgt gtcatcttcc 1320

cccagaaagg gtgcagtggc tgcagtgatg tacacggtgg tcacctccat gctcaacccc 1380

tttatctaca gcctgggaaa cagggatatt aaaagtgtct tg 1422

<210> 11

<211> 4135

<212> DNA

<213>mankind PLK1 gene

<400> 11

gggctctatg aaggatcaga gggtgctaag gaggcacaga gaaagagctt ttaacgtggg 60

tattttactg ttccagggtt gccactcaag tacctacaga acaaacgggt gtagctgtaa 120

actgtggcaa actgaaagca tgtaggcaca gagtaagtag ctagagctga tttttttcag 180

gaaaggccac aaatcccaac ttttatgagc tcctctttta aatgctgtaa atgttttaca 240

atggcggggc gcggtggctc acgcctgtaa tcccagcatt tgggaggccg aggggcagat 300

cacttgaggt caggagttca agaccagcct ggccaacatg gtgaaacctc ttctctacta 360

aaattacaaa aattagccgg gcatggtggt gcatgcttgt aattccagct gctagggagg 420

ccgaggcggg aggattgctt gaacccggga agcagaggtt gcagtgagct gagatcgtgc 480

cactgcactc cagcctgggc gacagagcaa gattccgtca cacacacaaa aaaaaggcgt 540

ggggggaggc caaacaaaac cccgcaagac acatttggct atgacctgcc agtttgctag 600

gcattcttcc aaccttccct ccctctgacc aagaaactga gtgtccacta ttttaggccc 660

tgggaaattc agtagcgagg aggccagaca gcttcgttgc atcatggggg gctctggtac 720

tgtgcttctc caacttcagg atgtgtagga atcacctgag cagtcttgtt gagaggcgga 780

cactgactcg ggaggtctgg ggtagggcct gaacgtttgc ctttgcggtt ctaacaagct 840

ctcaggtgat ggcgatgcta ctgttccctg gccccgaggt agaggaagat ttaagtaaaa 900

gcttcctgga ggaggcgcaa gtgaaccgca ggagctttcc cggacgcccg agaaagggag 960

aaaccccgaa ggaattcctc ctctctcggg gctgggtctc cgcatccacg ccgggtttgg 1020

tttcccaggc tatcccacgt gttcgggcgt ccgtgtcaat caggttttcc ccggctgggt 1080

ccgggtttaa aggctgctgc tgcgcagggc gctcccatgg tgccgcgcgg cgggcgggtt 1140

tggattttaa atccccgcgg ccaatcagtg gcgcgcaggc ttttgtaacg ttcccagcgc 1200

cgcgtttgaa ttcggggagg agcggagcgg tgcggaggct ctgctcggat cgaggtctgc 1260

agcgcagctt cgggagcatg agtgctgcag tgactgcagg gaagctggca cgggcaccgg 1320

ccgaccctgg gaaagccggg gtccccggag ttgcagctcc cggagctccg gcggcggctc 1380

caccggcgaa agagatcccg gaggtcctag tggacccacg cagccggcgg cgctatgtgc 1440

ggggccgctt tttgggcaag ggcggctttg ccaagtgctt cgagatctcg gacgcggaca 1500

ccaaggaggt gttcgcgggc aagattgtgc ctaagtctct gctgctcaag ccgcaccaga 1560

gggagaagat gtccatggaa atatccattc accgcagcct cgcccaccag cacgtcgtag 1620

gattccacgg ctttttcgag gacaacgact tcgtgttcgt ggtgttggag ctctgccgcc 1680

ggagggtgag tgtcgctgct ggggaactgg aactgcctgc ggggcagttg gagcgcccag 1740

acctggagct gctggaaaga gtacccagca agggagagcc tgggacttgg agctgctaga 1800

gaagggtgct gggagcctcc cgcttacgta tggaaagtgt ctttggtaag ggcttcttgg 1860

ctctgggagc tgctagagga gggggttcca gtgaagtgga gtctgagtca tgttgctgat 1920

agggaagtca gcttctggac gcgaggtgct ggggggaggg gtgctggtaa tggacgctag 1980

ggccctggag tacccaggga ggagtcccag gttagtgatg ccgcgccctg ggagcttctg 2040

gggtggagct gggtcagtgg gggggtagtt ggagccagac ttcgggcctt ccgggggaga 2100

tacgagtcag gaaagggatc tggtgctggg gactcggagc ttccggagtg gggctgagag 2160

aggaccccca ggtaaggggg gaagctagta ggagaagggg tgctgcgaat ggttgtggac 2220

agtgttaagg cagggtccag atgccgctgt gctggagaag gaatggggtg ggggcatagg 2280

gaaggagaga aacccaccaa gacccctctt tcatcccttg ggagtccaga gtccagtcct 2340

gtgcttcctt tgcctggtaa ccctctccct tccccaccgg cctcaatcca cctccccatc 2400

cctcctgccc tctccttccc acccacagtc tctcctggag ctgcacaaga ggaggaaagc 2460

cctgactgag cctgaggccc gatactacct acggcaaatt gtgcttggct gccagtacct 2520

gcaccgaaac cgagttattc atcgagacct caagctgggc aaccttttcc tgaatgaaga 2580

tctggaggtg aaaatagggg attttggact ggcaaccaaa gtcgaatatg acggggagag 2640

gaagaagacc ctgtgtggga ctcctaatta catagctccc gaggtgctga gcaagaaagg 2700

gcacagtttc gaggtggatg tgtggtccat tgggtgtatc atgtatacct tgttagtggg 2760

caaaccacct tttgagactt cttgcctaaa agagacctac ctccggatca agaagaatga 2820

atacagtatt cccaagcaca tcaaccccgt ggccgcctcc ctcatccaga agatgcttca 2880

gacagatccc actgcccgcc caaccattaa cgagctgctt aatgacgagt tctttacttc 2940

tggctatatc cctgcccgtc tccccatcac ctgcctgacc attccaccaa ggttttcgat 3000

tgctcccagc agcctggacc ccagcaaccg gaagcccctc acagtcctca ataaaggctt 3060

ggagaacccc ctgcctgagc gtccccggga aaaagaagaa ccagtggttc gagagacagg 3120

tgaggtggtc gactgccacc tcagtgacat gctgcagcag ctgcacagtg tcaatgcctc 3180

caagccctcg gagcgtgggc tggtcaggca agaggaggct gaggatcctg cctgcatccc 3240

catcttctgg gtcagcaagt gggtggacta ttcggacaag tacggccttg ggtatcagct 3300

ctgtgataac agcgtggggg tgctcttcaa tgactcaaca cgcctcatcc tctacaatga 3360

tggtgacagc ctgcagtaca tagagcgtga cggcactgag tcctacctca ccgtgagttc 3420

ccatcccaac tccttgatga agaagatcac cctccttaaa tatttccgca attacatgag 3480

cgagcacttg ctgaaggcag gtgccaacat cacgccgcgc gaaggtgatg agctcgcccg 3540

gctgccctac ctacggacct ggttccgcac ccgcagcgcc atcatcctgc acctcagcaa 3600

cggcagcgtg cagatcaact tcttccagga tcacaccaag ctcatcttgt gcccactgat 3660

ggcagccgtg acctacatcg acgagaagcg ggacttccgc acataccgcc tgagtctcct 3720

ggaggagtac ggctgctgca aggagctggc cagccggctc cgctacgccc gcactatggt 3780

ggacaagctg ctgagctcac gctcggccag caaccgtctc aaggcctcct aatagctgcc 3840

ctcccctccg gactggtgcc ctcctcactc ccacctgcat ctggggccca tactggttgg 3900

ctcccgcggt gccatgtctg cagtgtgccc cccagccccg gtggctgggc agagctgcat 3960

catccttgca ggtgggggtt gctgtataag ttatttttgt acatgttcgg gtgtgggttc 4020

tacagccttg tccccctccc cctcaacccc accatatgaa ttgtacagaa tatttctatt 4080

gaattcggaa ctgtcctttc cttggcttta tgcacattaa acagatgtga atatt 4135

<210> 12

<211> 3009

<212> DNA

<213>mankind HABP2 gene

<400> 12

cctgaatcct tggagactga catttttccc ccctaaaggc atagacaaca aaagaaattt 60

tattgagagg aaaacacaag tccttaaact gcaaagatgt ttgccaggat gtctgatctc 120

catgttctgc tgttaatggc tctggtggga aagacagcct gtgggttctc cctgatgtct 180

ttattggaaa gcctggaccc agactggacc cctgaccagt atgattacag ctacgaggat 240

tataatcagg aagagaacac cagtagcaca cttacccacg ctgagaatcc tgactggtac 300

tacactgagg accaagctga tccatgccag cccaacccct gtgaacacgg tggggactgc 360

ctcgtccatg ggagcacctt cacatgcagc tgcctggctc ctttctctgg gaataagtgt 420

cagaaagtgc aaaatacgtg caaggacaac ccatgtggcc ggggccaatg tctcattacc 480

cagagtcctc cctactaccg ctgtgtctgt aaacaccctt acacaggtcc cagctgctcc 540

caagtggttc ctgtatgcag gccaaacccc tgccagaatg gggctacctg ctcccggcat 600

aagcggagat ccaagttcac ctgtgcctgt cccgaccagt tcaaggggaa attctgtgaa 660

ataggttctg atgactgcta tgttggcgat ggctactctt accgagggaa aatgaatagg 720

acagtcaacc agcatgcgtg cctttactgg aactcccacc tcctcttgca ggagaattac 780

aacatgttta tggaggatgc tgaaacccat gggattgggg aacacaattt ctgcagaaac 840

ccagatgcgg acgaaaagcc ctggtgcttt attaaagtta ccaatgacaa ggtgaaatgg 900

gaatactgtg atgtctcagc ctgctcagcc caggacgttg cctacccaga ggaaagcccc 960

actgagccat caaccaagct tccggggttt gactcctgtg gaaagactga gatagcagag 1020

aggaagatca agagaatcta tggaggcttt aagagcacgg cgggcaagca cccatggcag 1080

gcgtccctcc agtcctcgct gcctctgacc atctccatgc cccagggcca cttctgtggt 1140

ggggcgctga tccacccctg ctgggtgctc actgctgccc actgcaccga cataaaaacc 1200

agacatctaa aggtggtgct aggggaccag gacctgaaga aagaagaatt tcatgagcag 1260

agctttaggg tggagaagat attcaagtac agccactaca atgaaagaga tgagattccc 1320

cacaatgata ttgcattgct caagttaaag ccagtggatg gtcactgtgc tctagaatcc 1380

aaatacgtga agactgtgtg cttgcctgat gggtcctttc cctctgggag tgagtgccac 1440

atctctggct ggggtgttac agaaacagga aaagggtccc gccagctcct ggatgccaaa 1500

gtcaagctga ttgccaacac tttgtgcaac tcccgccaac tctatgacca catgattgat 1560

gacagtatga tctgtgcagg aaatcttcag aaacctgggc aagacacctg ccagggtgac 1620

tctggaggcc ccctgacctg tgagaaggac ggcacctact acgtctatgg gatagtgagc 1680

tggggcctgg agtgtgggaa gaggccaggg gtctacaccc aagttaccaa attcctgaat 1740

tggatcaaag ccaccatcaa aagtgaaagt ggcttctaag gtactgtctt ctggacctca 1800

gagcccactc tccttggcac cctgacaccg ggaggcctca tggccaacaa tggacacctc 1860

cagagcctcc aggggaccac acagtagact atccctactc taagcagaga caactgccac 1920

ccagcctggg ccttcccaga ccagcatttg cacaatatca ccaggcttct tctgcctccc 1980

ttggtaaccc aaggaatgat ggaatcaaca caacatagta tgtttgcttt ccttacccaa 2040

ttgtaccttc tagaaaatca gtgttcacag agactgcctc caccacaggc atcctgcaaa 2100

tgcagactcc agaatcccca gcatcagcgg gaaccaccat cacatcttta ttcctcagcc 2160

cagacactcg aggcactcaa cagaatcagc catccacgtc taggtatcag agaggaccac 2220

aaatacaaca ttctccatct gctttcagag ttattatttt aataaaggaa gatctgggat 2280

gggctggtgg gccattccag cttgccgaaa tcaaagccat ctgaagcctg tctctggtga 2340

acaaacttcc tctctggcct ctcaggaatc agggtggaca tggctcacaa cagcagggcc 2400

ttcttctttt tgacgtgcag aatctcagtg gcatctgggt tcacctcccc actctgatga 2460

tctccagcct ccactgcttc tgccccccgc tgctgaaatc aaacataccc caagttaaaa 2520

tgaagctccc ccacccccac tcccggcccc ggttcccaca ggacacgcta agaagcacag 2580

ggagcattta acaggctcac cctccctttc cttttcccct cttctaccct ccccaagaaa 2640

aagggccttc aaggcaggaa tgagaaagca aagccaatct ctcatttaga cctggcttct 2700

ttcttctgaa caaagtaggg ttcaaaatgc agactgtcat atccagcgag tccctgaccc 2760

tttctgcgaa tgtaacgagc aagcagtcag cacagcctgg gctgccctgg cccgggattg 2820

atgtagcccc ggtaggtttg cctctgcaga actaatggct gtgacttcag agaaagccct 2880

gcaggaagtt taacctgcgt gtcatctgcc tggtcatctc agacccatga aattaggcgc 2940

cttgtttgag ctgcgtttca cacttcttta gagctagctg acctttggcc aaaaataaac 3000

tttgaaaag 3009

<210> 13

<211> 2118

<212> DNA

<213>mankind PBK gene

<400> 13

ataatcacga cacattcaac acagatgtga gcgtttggag agagcttcag ccttcagcac 60

aggcaactgt gttctattca ccgggctgtg ctcctcggaa gggctggtgg ttcctgtaaa 120

agtttgtgct ccccgcgcct ttccttcctt ccctcgtctt tggcgcctgc gggcaccggg 180

agtcctggga gagggaggag gaacggcgaa tcggcggggc gtgtccttca cgccgccccg 240

tagctcgggg ccgaagctgg cgcagcgcgc gactttttga aagccaggag ggttcgaatt 300

gcaacggcag ctgccgggcg tatgtgttgg tgctagaggc agctgcaggg tctcgctggg 360

ggccgctcgg gaccaatttt gaagaggtac ttggccacga cttattttca cctccgacct 420

ttccttccag gcggtgagac tctggactga gagtggcttt cacaatggaa gggatcagta 480

atttcaagac accaagcaaa ttatcagaaa aaaagaaatc tgtattatgt tcaactccaa 540

ctataaatat cccggcctct ccgtttatgc agaagcttgg ctttggtact ggggtaaatg 600

tgtacctaat gaaaagatct ccaagaggtt tgtctcattc tccttgggct gtaaaaaaga 660

ttaatcctat atgtaatgat cattatcgaa gtgtgtatca aaagagacta atggatgaag 720

ctaagatttt gaaaagcctt catcatccaa acattgttgg ttatcgtgct tttactgaag 780

ccaatgatgg cagtctgtgt cttgctatgg aatatggagg tgaaaagtct ctaaatgact 840

taatagaaga acgatataaa gccagccaag atccttttcc agcagccata attttaaaag 900

ttgctttgaa tatggcaaga gggttaaagt atctgcacca agaaaagaaa ctgcttcatg 960

gagacataaa gtcttcaaat gttgtaatta aaggcgattt tgaaacaatt aaaatctgtg 1020

atgtaggagt ctctctacca ctggatgaaa atatgactgt gactgaccct gaggcttgtt 1080

acattggcac agagccatgg aaacccaaag aagctgtgga ggagaatggt gttattactg 1140

acaaggcaga catatttgcc tttggcctta ctttgtggga aatgatgact ttatcgattc 1200

cacacattaa tctttcaaat gatgatgatg atgaagataa aacttttgat gaaagtgatt 1260

ttgatgatga agcatactat gcagcgttgg gaactaggcc acctattaat atggaagaac 1320

tggatgaatc ataccagaaa gtaattgaac tcttctctgt atgcactaat gaagacccta 1380

aagatcgtcc ttctgctgca cacattgttg aagctctgga aacagatgtc tagtgatcat 1440

ctcagctgaa gtgtggcttg cgtaaataac tgtttattcc aaaatattta catagttact 1500

atcagtagtt attagactct aaaattggca tatttgagga ccatagtttc ttgttaacat 1560

atggataact atttctaata tgaaatatgc ttatattggc tataagcact tggaattgta 1620

ctgggttttc tgtaaagttt tagaaactag ctacataagt actttgatac tgctcatgct 1680

gacttaaaac actagcagta aaacgctgta aactgtaaca ttaaattgaa tgaccattac 1740

ttttattaat gatctttctt aaatattcta tattttaatg gatctactga cattagcact 1800

ttgtacagta caaaataaag tctacatttg tttaaaacac tgaacctttt gctgatgtgt 1860

ttatcaaatg ataactggaa gctgaggaga atatgcctca aaaagagtag ctccttggat 1920

acttcagact ctggttacag attgtcttga tctcttggat ctcctcagat ctttggtttt 1980

tgctttaatt tattaaatgt attttccata ctgagtttaa aatttattaa tttgtacctt 2040

aagcatttcc cagctgtgta aaaacaataa aactcaaata ggatgataaa gaataaagga 2100

cactttgggt accagaag 2118

<210> 14

<211> 3021

<212> DNA

<213>mankind GPR37 gene

<400> 14

ggggttggaa tcccgcttct cctcggcagc agtaggctcg caagtcgctg gggttaggtg 60

gggcaagagt ttcgccggcg catcagcgct gcttcggact gtttgcaacg tgtttccagc 120

gagctgggag cggggttgtg actgcgagtc gtctggggga gggggacttg tttttctttt 180

cctctagaga cctcggcttg caactggatc aaacgctgtc gaaaggatgt aaataggcag 240

agcaactgtt accaagaagg ccaccacccc cacccaaagg cagtgaggag tgtggggctt 300

cgtctgggct cccccgagtc tcaacagtaa tcaacagtca ggtgttgatt gcaacttttc 360

aaggtcagcc accgggagta gcctattccc tctaggaacc ttggagggca taccttgctg 420

ggactcaact tggctgagaa atgcacaaga tgccaaagga ggaaggatta tagggggcgt 480

gtgtgtgacc cccaagaccg atcttccgct atcaccctaa tctccggttc cccgctaccc 540

gggcgggggt gagtatgtga catgtgccta actctcagca gcaacttcgg cagcaggtgt 600

cgatcctaac taagcaggag ctgcggctgc cgggtgtgcc ctcaccaagc catgcgagcc 660

ccgggcgcgc ttctcgcccg catgtcgcgg ctactgcttc tgctactgct caaggtgtct 720

gcctcttctg ccctcggggt cgcccctgcg tccagaaacg aaacttgtct gggggagagc 780

tgtgcaccta cagtgatcca gcgccgcggc agggacgcct ggggaccggg aaattctgca 840

agagacgttc tgcgagcccg agcacccagg gaggagcagg gggcagcgtt tcttgcggga 900

ccctcctggg acctgccggc ggccccgggc cgtgacccgg ctgcaggcag aggggcggag 960

gcgtcggcag ccggaccccc gggacctcca accaggccac ctggcccctg gaggtggaaa 1020

ggtgctcggg gtcaggagcc ttctgaaact ttggggagag ggaaccccac ggccctccag 1080

ctcttccttc agatctcaga ggaggaagag aagggtccca gaggcgctgg catttccggg 1140

cgtagccagg agcagagtgt gaagacagtc cccggagcca gcgatctttt ttactggcca 1200

aggagagccg ggaaactcca gggttcccac cacaagcccc tgtccaagac ggccaatgga 1260

ctggcggggc acgaagggtg gacaattgca ctcccgggcc gggcgctggc ccagaatgga 1320

tccttgggtg aaggaatcca tgagcctggg ggtccccgcc ggggaaacag cacgaaccgg 1380

cgtgtgagac tgaagaaccc cttctacccg ctgacccagg agtcctatgg agcctacgcg 1440

gtcatgtgtc tgtccgtggt gatcttcggg accggcatca ttggcaacct ggcggtgatg 1500

tgcatcgtgt gccacaacta ctacatgcgg agcatctcca actccctctt ggccaacctg 1560

gccttctggg actttctcat catcttcttc tgccttccgc tggtcatctt ccacgagctg 1620

accaagaagt ggctgctgga ggacttctcc tgcaagatcg tgccctatat agaggtcgct 1680

tctctgggag tcaccacttt caccttatgt gctctgtgca tagaccgctt ccgtgctgcc 1740

accaacgtac agatgtacta cgaaatgatc gaaaactgtt cctcaacaac tgccaaactt 1800

gctgttatat gggtgggagc tctattgtta gcacttccag aagttgttct ccgccagctg 1860

agcaaggagg atttggggtt tagtggccga gctccggcag aaaggtgcat tattaagatc 1920

tctcctgatt taccagacac catctatgtt ctagccctca cctacgacag tgcgagactg 1980

tggtggtatt ttggctgtta cttttgtttg cccacgcttt tcaccatcac ctgctctcta 2040

gtgactgcga ggaaaatccg caaagcagag aaagcctgta cccgagggaa taaacggcag 2100

attcaactag agagtcagat gaactgtaca gtagtggcac tgaccatttt atatggattt 2160

tgcattattc ctgaaaatat ctgcaacatt gttactgcct acatggctac aggggtttca 2220

cagcagacaa tggacctcct taatatcatc agccagttcc ttttgttctt taagtcctgt 2280

gtcaccccag tcctcctttt ctgtctctgc aaacccttca gtcgggcctt catggagtgc 2340

tgctgctgtt gctgtgagga atgcattcag aagtcttcaa cggtgaccag tgatgacaat 2400

gacaacgagt acaccacgga actcgaactc tcgcctttca gtaccatacg ccgtgaaatg 2460

tccacttttg cttctgtcgg aactcattgc tgaaggacag tacttggttg ggtcagattt 2520

atttgtttga ttttcatatc ccgtgaaagt ttttaattca tatttttcct tatagggaaa 2580

aatgcaaaaa agaaacaata aagaaagaaa tattaactac tgtagaactg attttacaaa 2640

ttaatatttg tgctttgaaa aaaagtttct atttagttat ttaagaagaa tgagaaggcc 2700

aatagtttta gattatttta tctggtatgg tgctaatatt ttatttgaaa aaagttactg 2760

caacttaact taaaattgct aacgtttttt cttcttttaa aaatacaatt attgtatatt 2820

gattatagca atgtgatttt gtaggttatt ttatatttga gttgtgattg aaagtatgtt 2880

gtatatggta ttgtgagatg atttgtactt ggaagcattc acaaagtagc accaaataaa 2940

ttacacttta ttctttaatg tcattgtcaa tctactttta accaatattc aataaatctt 3000

ctaattgcct taaagataca a 3021

<210> 15

<211> 2131

<212> DNA

<213>mankind NEK2 gene

<400> 15

cggggcccaa ggcaggggtg gcgggtcagt gctgctcggg ggcttctcca tccaggtccc 60

tggagttcct ggtccctgga gctccgcact tggcggcgca acctgcgtga ggcagcgcga 120

ctctggcgac tggccggcca tgccttcccg ggctgaggac tatgaagtgt tgtacaccat 180

tggcacaggc tcctacggcc gctgccagaa gatccggagg aagagtgatg gcaagatatt 240

agtttggaaa gaacttgact atggctccat gacagaagct gagaaacaga tgcttgtttc 300

tgaagtgaat ttgcttcgtg aactgaaaca tccaaacatc gttcgttact atgatcggat 360

tattgaccgg accaatacaa cactgtacat tgtaatggaa tattgtgaag gaggggatct 420

ggctagtgta attacaaagg gaaccaagga aaggcaatac ttagatgaag agtttgttct 480

tcgagtgatg actcagttga ctctggccct gaaggaatgc cacagacgaa gtgatggtgg 540

tcataccgta ttgcatcggg atctgaaacc agccaatgtt ttcctggatg gcaagcaaaa 600

cgtcaagctt ggagactttg ggctagctag aatattaaac catgacacga gttttgcaaa 660

aacatttgtt ggcacacctt attacatgtc tcctgaacaa atgaatcgca tgtcctacaa 720

tgagaaatca gatatctggt cattgggctg cttgctgtat gagttatgtg cattaatgcc 780

tccatttaca gcttttagcc agaaagaact cgctgggaaa atcagagaag gcaaattcag 840

gcgaattcca taccgttact ctgatgaatt gaatgaaatt attacgagga tgttaaactt 900

aaaggattac catcgacctt ctgttgaaga aattcttgag aaccctttaa tagcagattt 960

ggttgcagac gagcaaagaa gaaatcttga gagaagaggg cgacaattag gagagccaga 1020

aaaatcgcag gattccagcc ctgtattgag tgagctgaaa ctgaaggaaa ttcagttaca 1080

ggagcgagag cgagctctca aagcaagaga agaaagattg gagcagaaag aacaggagct 1140

ttgtgttcgt gagagactag cagaggacaa actggctaga gcagaaaatc tgttgaagaa 1200

ctacagcttg ctaaaggaac ggaagttcct gtctctggca agtaatccag aacttcttaa 1260

tcttccatcc tcagtaatta agaagaaagt tcatttcagt ggggaaagta aagagaacat 1320

catgaggagt gagaattctg agagtcagct cacatctaag tccaagtgca aggacctgaa 1380

gaaaaggctt cacgctgccc agctgcgggc tcaagccctg tcagatattg agaaaaatta 1440

ccaactgaaa agcagacaga tcctgggcat gcgctagcca ggtagagaga cacagagctg 1500

tgtacaggat gtaatattac caacctttaa agactgatat tcaaatgctg tagtgttgaa 1560

tacttggttc catgagccat gcctttctgt atagtacaca tgatatttcg gaattggttt 1620

tactgttctt cagcaactat tgtacaaaat gttcacattt aatttttctt tcttctttta 1680

agaacatatt ataaaaagaa tactttcttg gttgggcttt taatcctgtg tgtgattact 1740

agtaggaaca tgagatgtga cattctaaat cttgggagaa aaaataatgt taggaaaaaa 1800

atatttatgc aggaagagta gcactcactg aatagtttta aatgactgag tggtatgctt 1860

acaattgtca tgtctagatt taaattttaa gtctgagatt ttaaatgttt ttgagcttag 1920

aaaacccagt tagatgcaat ttggtcatta ataccatgac atcttgctta taaatattcc 1980

attgctctgt agttcaaatc tgttagcttt gtgaaaattc atcactgtga tgtttgtatt 2040

cttttttttt ttctgtttaa cagaatatga gctgtctgtc atttacctac ttctttccca 2100

ctaaataaaa gaattcttca gtttccctgt a 2131

<210> 16

<211> 366

<212> DNA

<213>mankind RP11-497G19.2 gene

<400> 16

ctcaagcaac acaatatcac atttccaggg cctcctcttc tcatcagcag gcagggacag 60

tgcatcgacc cagaggcctg ggagaggcag gaataatgat gcgccagaga gaattctggc 120

ttcgggagaa tcagagactt aacaaatata atgctgacaa tatgaccatt atgacacaag 180

aaactatttg tatccattgt cctttcctcg cctgaaataa cctattgctt cctactcggt 240

gatgacattt ttcaggcacc aagagttgcc cgtggaacaa gaagtacata aacttccttg 300

ctttgtagaa aacagattcc gttttataag tggtttagta tttagacact acctccaaag 360

gttggg 366

<210> 17

<211> 3667

<212> DNA

<213>mankind SLC2A1 gene

<400> 17

accattttgc tagagaaggc cgcggaggct cagagaggtg cgcacacttg ccctgagtca 60

cacagcgaat gccctccgcg gtcccaacgc agagagaacg agccgatcgg cagcctgagc 120

gaggcagtgg ttaggggggg ccccggcccc ggccactccc ctcaccccct ccccgcagag 180

cgccgcccag gacaggctgg gccccaggcc ccgccccgag gtcctgccca cacacccctg 240

acacaccggc gtcgccagcc aatggccggg gtcctataaa cgctacggtc cgcgcgctct 300

ctggcaagag gcaagaggta gcaacagcga gcgtgccggt cgctagtcgc gggtccccga 360

gtgagcacgc cagggagcag gagaccaaac gacgggggtc ggagtcagag tcgcagtggg 420

agtccccgga ccggagcacg agcctgagcg ggagagcgcc gctcgcacgc ccgtcgccac 480

ccgcgtaccc ggcgcagcca gagccaccag cgcagcgctg ccatggagcc cagcagcaag 540

aagctgacgg gtcgcctcat gctggccgtg ggaggagcag tgcttggctc cctgcagttt 600

ggctacaaca ctggagtcat caatgccccc cagaaggtga tcgaggagtt ctacaaccag 660

acatgggtcc accgctatgg ggagagcatc ctgcccacca cgctcaccac gctctggtcc 720

ctctcagtgg ccatcttttc tgttgggggc atgattggct ccttctctgt gggccttttc 780

gttaaccgct ttggccggcg gaattcaatg ctgatgatga acctgctggc cttcgtgtcc 840

gccgtgctca tgggcttctc gaaactgggc aagtcctttg agatgctgat cctgggccgc 900

ttcatcatcg gtgtgtactg cggcctgacc acaggcttcg tgcccatgta tgtgggtgaa 960

gtgtcaccca cagcccttcg tggggccctg ggcaccctgc accagctggg catcgtcgtc 1020

ggcatcctca tcgcccaggt gttcggcctg gactccatca tgggcaacaa ggacctgtgg 1080

cccctgctgc tgagcatcat cttcatcccg gccctgctgc agtgcatcgt gctgcccttc 1140

tgccccgaga gtccccgctt cctgctcatc aaccgcaacg aggagaaccg ggccaagagt 1200

gtgctaaaga agctgcgcgg gacagctgac gtgacccatg acctgcagga gatgaaggaa 1260

gagagtcggc agatgatgcg ggagaagaag gtcaccatcc tggagctgtt ccgctccccc 1320

gcctaccgcc agcccatcct catcgctgtg gtgctgcagc tgtcccagca gctgtctggc 1380

atcaacgctg tcttctatta ctccacgagc atcttcgaga aggcgggggt gcagcagcct 1440

gtgtatgcca ccattggctc cggtatcgtc aacacggcct tcactgtcgt gtcgctgttt 1500

gtggtggagc gagcaggccg gcggaccctg cacctcatag gcctcgctgg catggcgggt 1560

tgtgccatac tcatgaccat cgcgctagca ctgctggagc agctaccctg gatgtcctat 1620

ctgagcatcg tggccatctt tggctttgtg gccttctttg aagtgggtcc tggccccatc 1680

ccatggttca tcgtggctga actcttcagc cagggtccac gtccagctgc cattgccgtt 1740

gcaggcttct ccaactggac ctcaaatttc attgtgggca tgtgcttcca gtatgtggag 1800

caactgtgtg gtccctacgt cttcatcatc ttcactgtgc tcctggttct gttcttcatc 1860

ttcacctact tcaaagttcc tgagactaaa ggccggacct tcgatgagat cgcttccggc 1920

ttccggcagg ggggagccag ccaaagtgac aagacacccg aggagctgtt ccatcccctg 1980

ggggctgatt cccaagtgtg agtcgcccca gatcaccagc ccggcctgct cccagcagcc 2040

ctaaggatct ctcaggagca caggcagctg gatgagactt ccaaacctga cagatgtcag 2100

ccgagccggg cctggggctc ctttctccag ccagcaatga tgtccagaag aatattcagg 2160

acttaacggc tccaggattt taacaaaagc aagactgttg ctcaaatcta ttcagacaag 2220

caacaggttt tataattttt ttattactga ttttgttatt tttatatcag cctgagtctc 2280

ctgtgcccac atcccaggct tcaccctgaa tggttccatg cctgagggtg gagactaagc 2340

cctgtcgaga cacttgcctt cttcacccag ctaatctgta gggctggacc tatgtcctaa 2400

ggacacacta atcgaactat gaactacaaa gcttctatcc caggaggtgg ctatggccac 2460

ccgttctgct ggcctggatc tccccactct aggggtcagg ctccattagg atttgcccct 2520

tcccatctct tcctacccaa ccactcaaat taatctttct ttacctgaga ccagttggga 2580

gcactggagt gcagggagga gaggggaagg gccagtctgg gctgccgggt tctagtctcc 2640

tttgcactga gggccacact attaccatga gaagagggcc tgtgggagcc tgcaaactca 2700

ctgctcaaga agacatggag actcctgccc tgttgtgtat agatgcaaga tatttatata 2760

tatttttggt tgtcaatatt aaatacagac actaagttat agtatatctg gacaagccaa 2820

cttgtaaata caccacctca ctcctgttac ttacctaaac agatataaat ggctggtttt 2880

tagaaacatg gttttgaaat gcttgtggat tgagggtagg aggtttggat gggagtgaga 2940

cagaagtaag tggggttgca accactgcaa cggcttagac ttcgactcag gatccagtcc 3000

cttacacgta cctctcatca gtgtcctctt gctcaaaaat ctgtttgatc cctgttaccc 3060

agagaatata tacattcttt atcttgacat tcaaggcatt tctatcacat atttgatagt 3120

tggtgttcaa aaaaacacta gttttgtgcc agccgtgatg ctcaggcttg aaatgcatta 3180

ttttgaatgt gaagtaaata ctgtaccttt attggacagg ctcaaagagg ttatgtgcct 3240

gaagtcgcac agtgaataag ctaaaacacc tgcttttaac aatggtacca tacaaccact 3300

actccattaa ctccacccac ctcctgcacc cctccccaca cacacaaaat gaaccacgtt 3360

ctttgtatgg gcccaatgag ctgtcaagct gccctgtgtt catttcattt ggaattgccc 3420

cctctggttc ctctgtatac tactgcttca tctctaaaga cagctcatcc tcctccttca 3480

cccctgaatt tccagagcac ttcatctgct ccttcatcac aagtccagtt ttctgccact 3540

agtctgaatt tcatgagaag atgccgattt ggttcctgtg ggtcctcagc actattcagt 3600

acagtgcttg atgcacagca ggcactcaga aaatactgga ggaaataaaa caccaaagat 3660

atttgtc 3667

<210> 18

<211> 3043

<212> DNA

<213>mankind PLOD2 gene

<400> 18

aagtgctgac tgggaccgac agtgagggag gggagcccag agggaagttg ataacccaaa 60

tgtcatctgg tatctccagt tgttcattcc ttgatattgc tgatgataat gttacaaact 120

gtaccaggga aagcaaacat agtcactgcc cctggtaaga aggcagggga ataactatct 180

tagaaaattt gatgaaaatc aagtttgtaa aatattaata cctgaaaagt catatactta 240

catctccaac attcatttaa aactgagttg tgggatatgt aagttgcatt tgtgtgcatg 300

tgtatgtatg tatgtatgta tgcagaaaca ttttgatttg tggatgtttt atgccttaaa 360

atatttactg gcttgaaaat atattttaac tggcttaaaa ttttagaggt gagctccctg 420

tgaatatccg tgttaaattg accattttga aatttaagac aatatatata tcgtttcaga 480

tattgttcca ctattttgat cagaaaagta tgctagagaa ccatatttta cataaaagaa 540

tatatatatt aacatttttc agtcagcaga tatttatttt atgtcatgcc catttcattt 600

tctttttcac tgttagggac ttttgccgtc aggatgaaaa gtgtgattat tactttagtg 660

tggatgcaga tgttgttttg acaaatccaa ggactttaaa aattttgatt gaacaaaaca 720

gaaagatcat tgctcctctt gtaactcgtc atggaaagct gtggtccaat ttctggggag 780

cattgagtcc tgatggatac tatgcacgat ctgaagatta tgtggatatt gttcaaggga 840

atagagtagg agtatggaat gtcccatata tggctaatgt gtacttaatt aaaggaaaga 900

cactccgatc agagatgaat gaaaggaact attttgttcg tgataaactg gatcctgata 960

tggctctttg ccgaaatgct agagaaatga ctttacaaag ggaaaaagac tcccctactc 1020

cggaaacatt ccaaatgctc agccccccaa agggtgtatt tatgtacatt tctaatagac 1080

atgaatttgg aaggctatta tccactgcta attacaatac ttcccattat aacaatgacc 1140

tctggcagat ttttgaaaat cctgtggact ggaaggaaaa gtatataaac cgtgattatt 1200

caaagatttt cactgaaaat atagttgaac agccctgtcc agatgtcttt tggttcccca 1260

tattttctga aaaagcctgt gatgaattgg tagaagaaat ggaacattac ggcaaatggt 1320

ctgggggaaa acatcatgat agccgtatat ctggtggtta tgaaaatgtc ccaactgatg 1380

atatccacat gaagcaagtt gatctggaga atgtatggct tcattttatc cgggagttca 1440

ttgcaccagt tacactgaag gtctttgcag gctattatac gaagggattt gcactactga 1500

attttgtagt aaaatactcc cctgaacgac agcgttctct tcgtcctcat catgatgctt 1560

ctacatttac cataaacatt gcacttaata acgtgggaga agactttcag ggaggtggtt 1620

gcaaatttct aaggtacaat tgctctattg agtcaccacg aaaaggctgg agcttcatgc 1680

atcctgggag actcacacat ttgcatgaag gacttcctgt taaaaatgga acaagataca 1740

ttgcagtgtc atttatagat ccctaagtta tttacttttc attgaattga aatttatttt 1800

ggatgaatga ctggcatgaa cacgtctttg aagttgtggc tgagaagatg agaggaatat 1860

ttaaataaca tcaacagaac aacttcactt tgggccaaac atttgaaaaa ctttttataa 1920

aaaattgttt gatatttctt aatgtctgct ctgagcctta aaacacagat tgaagaagaa 1980

aagaaagaaa aaacttaaat atttatttct atgctttgtt gcctctgaga ataatgacaa 2040

tttatgaatt tgtgtttcaa attgataaaa tatttaggta caaataacaa gactaataat 2100

attttcttat ttaaaaaaag catgggaaga tttttattta tcaaaatata gaggaaatgt 2160

agacaaaatg gatataaatg aaaattacca tgttgtaaaa ccttgaaaat cagattctaa 2220

ctggatttgt atgcaactaa gtatttttct gaacacctat gcaggtctta tttacagtag 2280

ttactaaggg aacacacaaa gaattacaca acgttttcct caagaaaatg gtacaaaaca 2340

caaccgagga gcgtatacag ttgaaaacat ttttgttttg attggaaggc agattatttt 2400

atattagtat taaaaatcaa accctatgtt tctttcagat gaatcttcca aagtggatta 2460

tattaagcag gtattagatt taggaaaacc tttccatttc ttaaagtatt atcaagtgtc 2520

aagatcagca agtgtcctta agtcaaacag gttttttttg ttgttgtttt tgctttgttt 2580

ccttttttag aaagttctag aaaataggaa aacgaaaaat ttcattgaga tgagtagtgc 2640

atttaattat tttttaaaaa actttttaag tacttgaatt ttatatcagg aaaacaaagt 2700

tgttgagcct tgcttcttcc gttttgccct ttgtctcgct ccttattctt tttttggggg 2760

gagggttatt tgctttttta tcttcctggc ataatttcca ttttattctt ctgagtgtct 2820

atgttaactt ccctctatcc cgcttataaa aaaattctcc aacaaaaata cttgttgact 2880

tgatgtttta tcacttctct aagtaaggtt gaaatatcct tattgtagct actgttttta 2940

atgtaaaggt taaacttgaa aagaaattct taatcacggt gccaaaattc attttctaac 3000

accatgtgtt agaaaattat aaaaaataaa ataattttag aaa 3043

<210> 19

<211> 4087

<212> DNA

<213>mankind ECT2 gene

<400> 19

tctggagact cctgcctatg ggaaagaggt tgacatcacg cgccaatcgg catggctctt 60

agagagagca gcttagtttt tgaatcggtt gtggcggccg ccggcgagga atggcggtat 120

ttgtgagagg agtcggcgtt tgaagaggtg gaactcctag ggcttttttg agagtgctga 180

tttagaagaa tacaaatcat ggctgaaaat agtgtattaa catccactac tgggaggact 240

agcttggcag actcttccat ttttgattct aaagttactg agatttccaa ggaaaactta 300

cttattggat ctacttcata tgtagaagaa gagatgcctc agattgaaac aagagtgata 360

ttggttcaag aagctggaaa acaagaagaa cttataaaag ccttaaagga cattaaagtg 420

ggctttgtaa agatggagtc agtggaagaa tttgaaggtt tggattctcc ggaatttgaa 480

aatgtatttg tagtcacgga ctttcaggat tctgtcttta atgacctcta caaggctgat 540

tgtagagtta ttggaccacc agttgtatta aattgttcac aaaaaggaga gcctttgcca 600

ttttcatgtc gcccgttgta ttgtacaagt atgatgaatc tagtactatg ctttactgga 660

tttaggaaaa aagaagaact agtcaggttg gtgacattgg tccatcacat gggtggagtt 720

attcgaaaag actttaattc aaaagttaca catttggtgg caaattgtac acaaggagaa 780

aaattcaggg ttgctgtgag tctaggtact ccaattatga agccagaatg gatttataaa 840

gcttgggaaa ggcggaatga acaggatttc tatgcagcag ttgatgactt tagaaatgaa 900

tttaaagttc ctccatttca agattgtatt ttaagtttcc tgggattttc agatgaagag 960

aaaaccaata tggaagaaat gactgaaatg caaggaggta aatatttacc gcttggagat 1020

gaaagatgca ctcaccttgt agttgaagag aatatagtaa aagatcttcc ctttgaacct 1080

tcaaagaaac tttatgttgt caagcaagag tggttctggg gaagcattca aatggatgcc 1140

cgagctggag aaactatgta tttatatgaa aaggcaaata ctcctgagct caagaaatca 1200

gtgtcaatgc tttctctaaa tacccctaac agcaatcgca aacgacgtcg tttaaaagaa 1260

acacttgctc agctttcaag agagacagac gtgtcaccat ttccaccccg taagcgccca 1320

tcagctgagc attccctttc catagggtca ctcctagata tctccaacac accagagtct 1380

agcattaact atggagacac cccaaagtct tgtactaagt cttctaaaag ctccactcca 1440

gttccttcaa agcagtcagc aaggtggcaa gttgcaaaag agctttatca aactgaaagt 1500

aattatgtta atatattggc aacaattatt cagttatttc aagtaccatt ggaagaggaa 1560

ggacaacgtg gtggacctat ccttgcacca gaggagatta agactatttt tggtagcatc 1620

ccagatatct ttgatgtaca cactaagata aaggatgatc ttgaagacct tatagttaat 1680

tgggatgaga gcaaaagcat tggtgacatt tttctgaaat attcaaaaga tttggtaaaa 1740

acctaccctc cctttgtaaa cttctttgaa atgagcaagg aaacaattat taaatgtgaa 1800

aaacagaaac caagatttca tgcttttctc aagataaacc aagcaaaacc agaatgtgga 1860

cggcagagcc ttgttgaact tcttatccga ccagtacaga ggttacccag tgttgcatta 1920

cttttaaatg atcttaagaa gcatacagct gatgaaaatc cagacaaaag cactttagaa 1980

aaagctattg gatcactgaa ggaagtaatg acgcatatta atgaggataa gagaaaaaca 2040

gaagctcaaa agcaaatttt tgatgttgtt tatgaagtag atggatgccc agctaatctt 2100

ttatcttctc accgaagctt agtacagcgg gttgaaacaa tttctctagg tgagcacccc 2160

tgtgacagag gagaacaagt aactctcttc ctcttcaatg attgcctaga gatagcaaga 2220

aaacggcaca aggttattgg cacttttagg agtcctcatg gccaaacccg acccccagct 2280

tctcttaagc atattcacct aatgcctctt tctcagatta agaaggtatt ggacataaga 2340

gagacagaag attgccataa tgcttttgcc ttgcttgtga ggccaccaac agagcaggca 2400

aatgtgctac tcagtttcca gatgacatca gatgaacttc caaaagaaaa ctggctaaag 2460

atgctgtgtc gacatgtagc taacaccatt tgtaaagcag atgctgagaa tcttatttat 2520

actgctgatc cagaatcctt tgaagtaaat acaaaagata tggacagtac attgagtaga 2580

gcatcaagag caataaaaaa gacttcaaaa aaggttacaa gagcattctc tttctccaaa 2640

actccaaaaa gagctcttcg aagggctctt atgacatccc acggctcagt ggagggaaga 2700

agtccttcca gcaatgataa gcatgtaatg agtcgtcttt ctagcacatc atcattagca 2760

ggtatccctt ctccctccct tgtcagcctt ccttccttct ttgaaaggag aagtcatacg 2820

ttaagtagat ctacaactca tttgatatga agcgttacca aaatcttaaa ttatagaaat 2880

gtatagacac ctcatactca aataagaaac tgacttaaat ggtacttgta attagcactt 2940

ggtgaaagct ggaaggaaga taaataacac taaactatgc tatttgattt ttcttcttga 3000

aagagtaagg tttacctgtt acattttcaa gttaattcat gtaaaaaatg atagtgattt 3060

tgatgtaatt tatctcttgt ttgaatctgt cattcaaagg ccaataattt aagttgctat 3120

cagctgatat tagtagcttt gcaaccctga tagagtaaat aaattttatg ggcgggtgcc 3180

aaatactgct gtgaatctat ttgtatagta tccatgaatg aatttatgga aatagatatt 3240

tgtgcagctc aatttatgca gagattaaat gacatcataa tactggatga aaacttgcat 3300

agaattctga ttaaatagtg ggtctgtttc acatgtgcag tttgaagtat ttaaataacc 3360

actcctttca cagtttattt tcttctcaag cgttttcaag atctagcatg tggattttaa 3420

aagatttgcc ctcattaaca agaataacat ttaaaggaga ttgtttcaaa atatttttgc 3480

aaattgagat aaggacagaa agattgagaa acattgtata ttttgcaaaa acaagatgtt 3540

tgtagctgtt tcagagagag tacggtatat ttatggtaat tttatccact agcaaatctt 3600

gatttagttt gatagtgtgt ggaattttat tttgaaggat aagaccatgg gaaaattgtg 3660

gtaaagactg tttgtaccct tcatgaaata attctgaagt tgccatcagt tttactaatc 3720

ttctgtgaaa tgcatagata tgcgcatgtt caacttttta ttgtggtctt ataattaaat 3780

gtaaaattga aaattcattt gctgtttcaa agtgtgatat ctttcacaat agccttttta 3840

tagtcagtaa ttcagaataa tcaagttcat atggataaat gcatttttat ttcctatttc 3900

tttagggagt gctacaaatg tttgtcactt aaatttcaag tttctgtttt aatagttaac 3960

tgactataga ttgttttcta tgccatgtat gtgccacttc tgagagtagt aaatgactct 4020

ttgctacatt ttaaaagcaa ttgtattagt aagaactttg taaataaata cctaaaaccc 4080

aagtgta 4087

<210> 20

<211> 510

<212> DNA

<213>mankind SPC25 gene

<400> 20

agaaaagctg tctgtgaaat taaaggaaga agaacgaatg gttgagatgt ttctggaata 60

tcaaaatcag atcagcaggc aaaataagct cattcaagaa aaaaaggata acttgttaaa 120

attgattgct gaagtaaaag gcaaaaagca ggaattggaa gtactgactg caaatatcca 180

ggatcttaag gaagaatatt ctaggaagaa ggaaactatt tctactgcta ataaagcgaa 240

tgcagagagg ttgaaaaggc tgcagaaatc tgcagacttg tataaagatc gacttggact 300

agaaattcga aaaatttatg gtgagaaatt gcagtttatt ttcactaata ttgaccctaa 360

gaatcctgag agcccattta tgttttcctt acatctcaat gaagcaaggg actatgaaga 420

gtaaaaaaca tacaaacaag aaaaaaagga gcagaggctg atgccaagag aaaactctcc 480

tcttttggaa caaagcacat tcatgtgcta 510

<210> 21

<211> 1036

<212> DNA

<213>mankind RP5-839B4.8 gene

<400> 21

gggacttttg aaatgcattc tgtgaatgag aagaaatgca aacatgttgg caaaactgat 60

tattttcatt gtggatctca cgcttgcttt ggccattttt gttatctttt atttaatggc 120

tgccactgtt tttttcatct cggtcatttt ctatattttc aaaagggaga tggaaaaaaa 180

actttgactt caacaagcgc cgaatctggg cgctggattc tagcaatgct actgaggagc 240

ctttggcagg tggcgcagag aaagtcagct ccaagctgct tcctgtccat tgtccagctg 300

taggagaagg gtctttcctg cccaaggaca gtggtcctct cttctaaaga gcaaggagct 360

ctctgaggtg gaggaggctt gtgaagactt cccagaacac tgcacccacc tgaccaagag 420

gtacttttta aggtcattca aactgtcatc tacacatgaa taacaatgaa attctcataa 480

agaagcagaa atacccagga ggcatgaaga gtcatcatgc ttcaaaagta catgtattag 540

gacttcacga cctcaggaaa agtagactcc gtaactctta aaagctcaat cttggagagg 600

agaaacatgc tgtttgatga catagcacag aaacttgttg aaatatcctt ggagcttgag 660

atgtcgagag cgagccatca aaatgttctc aagaattaaa ttgaaaaatg ttgcttcaaa 720

atgtcttagc caaccaaata caaacatttt tgtcattgtg acaaaaaaaa agctcaccaa 780

ttgtctttgc tagtctcagc acagcccaag aggagcatcc ttcatgagcc aactgaaaaa 840

cagtgtgtag tctatgcttc tgaccagatg ggcttctctt gtcaccataa cattatgtat 900

taggacttca tgtattatgt attatgtcaa cactttattc attatgctga tcatactctg 960

tatttcactt gctctggtta tttgtaaagc ttttcctatt tcatcattaa attatccttg 1020

tattttagca actgca 1036

<210> 22

<211> 2745

<212> DNA

<213>mankind SKA3 gene

<400> 22

agtggcggga ggctgtgagc tgagcggtgg ggtctgcgta cgcctggagt ccttccccgc 60

tgtgctcagc atggacccta tccggagctt ctgcgggaag ctgcggtctc tggccagcac 120

gctggactgc gagacggccc ggctgcagcg agcgctggac ggagaggaaa gcgactttga 180

agattatcca atgagaattt tatatgacct tcattcagaa gttcagactc taaaggatga 240

tgttaatatt cttcttgata aagcaagatt ggaaaatcaa gaaggcattg atttcataaa 300

ggcaacaaaa gtactaatgg aaaaaaattc aatggatatt atgaaaataa gagagtattt 360

ccagaagtat ggatatagtc cacgtgtcaa gaaaaattca gtacacgagc aagaagccat 420

taactctgac ccagagttgt ctaattgtga aaattttcag aagactgatg tgaaagatga 480

tctgtctgat cctcctgttg caagcagttg tatttctgag aagtctccac gtagtccaca 540

actttcagat tttggacttg agcggtacat cgtatcccaa gttctaccaa accctccaca 600

ggcagtgaac aactataagg aagagcccgt aattgtaacc ccacctacca aacaatcact 660

agtaaaagta ctaaaaactc caaaatgtgc actaaaaatg gatgattttg agtgtgtaac 720

tcctaaatta gaacactttg gtatctctga atatactatg tgtttaaatg aagattacac 780

aatgggactt aaaaatgcga ggaataataa aagtgaggag gccatagata cagaatccag 840

gctcaatgat aatgtttttg ccactcccag ccccatcatc cagcagttgg aaaaaagtga 900

tgccgaatat accaactctc ctttggtacc tacattctgt actcctggtt tgaaaattcc 960

atctacaaag aacagcatag ctttggtatc cacaaattac ccattatcaa aaacaaatag 1020

ttcatcaaat gatttggaag ttgaagatcg tacttcgttg gttttaaatt cagacacatg 1080

ctttgagaat ttaacagatc cctcttcacc tacgatttct tcttatgaga atctgctcag 1140

aacacctaca cctccggaag taactaaaat tccagaagat attctccaga aattccagtg 1200

gatctatcca acacagaaac tgaacaaaat gagatgaaag ccgagctgga ccgattttaa 1260

cattcacatt gccctgcctc tgtccccctt taaacgttga cccattttaa agacaaacat 1320

gaacattaac atcataatat gctttttatg aagtttcaat aaggtttaac cttagtcttg 1380

ttgacatgta gcccagtcat tcactcttta aggattatta gtgtttcatt gatactaaat 1440

tacccagctt aatcaacaga atggtttaag tagtaccagg aagtaggaca agtaatttca 1500

aaaatataaa ggtgtttgct actcagatga ggccgcccct gaccttctgg ccagagagac 1560

attgctgcca gccagctctg ccttcccatc atctcctttc aggaccgtcc cacacctttt 1620

acttgctcag tgctgtctga agatgcagtt gctgtttgca aacaacagga acaccagtta 1680

aactaattag gaaaagaggg agatttccag gcctgggtaa ctatatactg tgaccattgg 1740

aggtagagac aggtctcaac agttggaacc aggaactctg ctgtcaggtt gagagttttg 1800

tttctcttcc agcttttcac tgtgtggggg tcttttctct tatgtcagct ctttctatca 1860

catggcagct gacctctcac gctccactct gcagcttgga cacccagtag accctgaatt 1920

tcactctctc taaaaggttc tgagggctca tcctgggcca ggggccctcc tgtgcactgt 1980

tagctatggc cacgggagcc tccagagctg cctggtagct tcaggttgac ctgcttatca 2040

ggcctacgat ccttctgatt taagtacagc tggaaagtat tatctaatta agttcatgat 2100

agtgcttttg gagaacttgt caaattacag ccaatgagaa aataaggacc tagcatactg 2160

tggagaacca ttaaaaattt gagaagaaac aacaagtatt atgtcaactt acttcaaagg 2220

cgtagttttg ggaatttgat gcagtaaaga ttaccctgtt ttatgattgt tccttgaaag 2280

tcaaatgggg gacctgtcca ttgtgctcta ttaatcttgt cagaaaactg tcaccaaaac 2340

aaaacttgag tttgtccttg ttctaggagt tactgggtag ttgtaagtat tatttttatt 2400

aaatataatg taaaataaaa tgttaagata cttagttttg tttttcaaag taaagctgta 2460

gtcagcctta tgtatgccat tgactctgaa atgtatacca gcctttcact gtgtaccgtg 2520

tgtatataaa tccacagaac cggatgagct gcttagggag ggaatatatt caaagtgtac 2580

caaggaccaa atcctggagt tctcccaact ttagaggatg gaaaggggca gagtaatcta 2640

gcaaaggaga ctgaggccag tgaagtagga agaaagtatt tcaaggagag tgatgattct 2700

gtgaatattg ctgagaattc aaataaaaag aggactgaga actga 2745

<210> 23

<211> 2732

<212> DNA

<213>mankind C20orf197 gene

<400> 23

atatcctgcc tgagcctggg ccgggtggag gtgtcctgca tggagtcttg tgaaatcgcg 60

tggactgcaa cacccagcag cgcgccgccc tgggtcctgc cactgagcag ctgttggtag 120

gctctgctgt gaggtctcct cttccggctt tcccacttcc caagtgttgg ttaaggaatc 180

cagatgttac tgcaataacc actccataaa caaaaggaac acccagctgt gagaactggc 240

ttctcagcat tcgtcccagc agaggctctt ctggggccag ccctggaaga acccatcagg 300

gttctgatgg ttgccctgtt tcagcacagc ccttattggc aggcagacgg ctacgggcac 360

agccacaggc tgaagtgtca acacttcaaa caacacagac agtacaatga caaattggag 420

atcagctcta atctcggccc ccaatttaat gcattgctga atattcttct gaacatagtc 480

catcccacac tgtcccatga cacaagacgc tccaaggggc tgaagataga gggacttctg 540

cagtcaagag agctgggaaa ctcttggaca gtcacaatgt gcatttgggt attaaaggct 600

ctgcaaagtt ctgcaccaaa taaacccttg gattggcttg atccaatgcc atgtttccaa 660

aacctacttg cccgtgggac accttagtcc ataacacagg ttggcatttc ttctagagag 720

tgtgctgtga aaaacactgg tctcacagca ccgtgcattc atccagcagg tatttaccaa 780

gcagggactt tgggccaggt ccgtgctagg ctctgcaggt ggaccagcca gccctgacct 840

ccatggtgtc tcttctcatg ggagaggctg cacagcagtc attgagaaaa cgaagaaaca 900

cacaggtact ttcagatgct gataatgact accatgtgct aaaagagctc caggtgttgt 960

ctgttttgag acaatcttct cgacaatgag atagaatgaa ccatgcaaac tttgggggct 1020

acgatggttt taggaaagag ctagagtgaa aaatcctttg acatatatac atacaaataa 1080

agatacatgt gtataatttt atgtaattga catcacatta tatgctggca cctgcctttt 1140

ttgcttaatg aaaatgtcaa aggtaatatt accaatcaat caatatattt caccatcgtg 1200

tttaatttct gcagagcact tattcaagca catactgcat ttttcttagt cactcactgg 1260

tgggaataga aagttttctc aggcatgcat ttttcccact tctgaaatga tctccttaga 1320

ataaattccc caaattgtat tgctgggttc aaaggcatga acattttaca tttttataca 1380

ataatgtcaa actaccttcc ggaaatgatg ctcactttac tttccctcca agactgtgtg 1440

aaaatgccca ttttcctaaa tgcttactat gactggtttc caactacatt ttaattcttg 1500

ttcaatctga taggcaaaaa atgatattta atttttattt gattgataat gaccttgaac 1560

gtgcccatta gccttttgca tgtattcttt tatgaaacat ctgttcctat ccttcgtcaa 1620

tgttcctctt catatgttaa cttttcttat tgatttgtta gagcactttg tatattgtga 1680

atattagctt ttgtgatcag aattatggaa ttgttgttgt ctttagtttt gcttatcaag 1740

tttaaagcca ttcagagatg ttgtaaatgt gtatgttgtt aaatttatgt cttattttgt 1800

gttttctgat gctcatatgt ttagaatggt caagcaatcc caacttatga ttacttaaat 1860

attctcccat atttgctctt agcatttttt ttcttttcat gtaacccttt gttctttcag 1920

gaatttactt tgcggtaaga aatgggctgg cttccagttt tatgtttccc aaatggtgat 1980

tcagctgttc catttccata ttctccctta ttagaaatga ccactatatt atgttctaaa 2040

atatctgcgt acttgtgtcc cttcctataa tctcagttca tctctttgag ctatcttttg 2100

attccttttt caaaccacac tgctttactg aactgtcatc atcttataca tttttaatac 2160

tcagcaagac aagtttctca atgccactct ttttcagagt ttttctggtg gttgtaagat 2220

gtttattctt ctggataaac tttagaatca ctctttttgt ccaaggtaaa atatatccca 2280

cattgagatc atactgaata tacagactaa ttcaggaaaa aatgtatgtc tttattgcat 2340

tgagtcttct tatccaataa aaaagatatg aatttccatg tattgaaatc ttcactgaga 2400

cttatttttg gcttttcaca tgtcctgcaa atgtattgtt aaatttattt ttaggtattt 2460

taggggaaat gattttctaa agtttgtatt ttctagcttg ttataattta catatgagat 2520

agtcattgtt gtatattatt tataactgat catattactg tatttgtatt gttttaatag 2580

tttttctatt attttgggtt ttcctggaat acaaccttat tatctacaaa ttatgattgt 2640

tttgcctttt ccaatgttca taactgtttt tatattcttg tctgattgct ttgttcagca 2700

cttctagaat aaagtcatgc aatactaatg at 2732

<210> 24

<211> 1694

<212> DNA

<213>mankind LYPD3 gene

<400> 24

gcgaggttac tcatcctggg ctcaggtaag agggcccgag ctcggaggcg gcacatccag 60

gggggacgcc aagggagcag gacggagcca tggaccccgc caggaaagca ggtgcccagg 120

ccatgatctg gactgcaggc tggctgctgc tgctgctgct tcgcggagga gcgcaggccc 180

tggagtgcta cagctgcgtg cagaaagcag atgacggatg ctccccgaac aagatgaaga 240

cagtgaagtg cgcgccgggc gtggacgtct gcaccgaggc cgtgggggcg gtggagacca 300

tccacggaca attctcgctg gcagtgcggg gttgcggttc gggactcccc ggcaagaatg 360

accgcggcct ggatcttcac gggcttctgg cgttcatcca gctgcagcaa tgcgctcagg 420

atcgctgcaa cgccaagctc aacctcacct cgcgggcgct cgacccggca ggtaatgaga 480

gtgcataccc gcccaacggc gtggagtgct acagctgtgt gggcctgagc cgggaggcgt 540

gccagggtac atcgccgccg gtcgtgagct gctacaacgc cagcgatcat gtctacaagg 600

gctgcttcga cggcaacgtc accttgacgg cagctaatgt gactgtgtcc ttgcctgtcc 660

ggggctgtgt ccaggatgaa ttctgcactc gggatggagt aacaggccca gggttcacgc 720

tcagtggctc ctgttgccag gggtcccgct gtaactctga cctccgcaac aagacctact 780

tctcccctcg aatcccaccc cttgtccggc tgccccctcc agagcccacg actgtggcct 840

caaccacatc tgtcaccact tctacctcgg ccccagtgag acccacatcc accaccaaac 900

ccatgccagc gccaaccagt cagactccga gacagggagt agaacacgag gcctcccggg 960

atgaggagcc caggttgact ggaggcgccg ctggccacca ggaccgcagc aattcagggc 1020

agtatcctgc aaaagggggg ccccagcagc cccataataa aggctgtgtg gctcccacag 1080

ctggattggc agcccttctg ttggccgtgg ctgctggtgt cctactgtga gcttctccac 1140

ctggaaattt ccctctcacc tacttctctg gccctgggta cccctcttct catcacttcc 1200

tgttcccacc actggactgg gctggcccag cccctgtttt tccaacattc cccagtatcc 1260

ccagcttctg ctgcgctggt ttgcggcttt gggaaataaa ataccgttgt atatattctg 1320

ccaggggtgt tctagctttt tgaggacagc tcctgtatcc ttctcatcct tgtctctccg 1380

cttgtcctct tgtgatgtta ggacagagtg agagaagtca gctgtcacgg ggaaggtgag 1440

agagaggatg ctaagcttcc tactcacttt ctcctagcca gcctggactt tggagcgtgg 1500

ggtgggtggg acaatggctc cccactctaa gcactgcctc ccctactccc cgcatctttg 1560

gggaatcggt tccccatatg tcttccttac tagactgtga gctcctcgag ggcagggacc 1620

gtgccttatg tctgtgtgtg atcagtttct ggcacataaa tgcctcaata aagatttaat 1680

tactttgtat agtg 1694

<210> 25

<211> 5740

<212> DNA

<213>mankind E2F7 gene

<400> 25

gttttagcgg ggactacgat ccaggctgga gttgcgctcg gccggtctga gcgctggcgc 60

tgcccggacg ccgcggggtc cccgccagcc cagggcactc ggcgcgggga tctgcgcgcc 120

tcgctctccc ttcccgatgc cgccgcccgg ctgctgatcg ccgcaccacc ttccctcatc 180

ggcttgggtc cgtggaggtc cctgcagagg caggaagcct ccttaggaaa gcagggatgg 240

aggtaaattg tttaacacta aaagacctga tcagccccag gcagcccaga ctagattttg 300

cagttgaaga tggggaaaat gcacaaaagg aaaatatatt tgttgatcga tcaaggatgg 360

ccccgaagac tccaataaaa aatgaaccaa ttgatttatc gaagcaaaaa aaatttactc 420

cagaaagaaa tcccattact ccagttaagt ttgttgacag acagcaagcg gaaccatgga 480

cacccacagc taacctgaag atgctcatta gtgctgccag cccagatata agggaccggg 540

agaagaaaaa gggactattc cgacccattg aaaacaagga cgatgcattt acagattctc 600

tacagcttga tgttgttggg gacagtgctg tggacgaatt tgaaaagcaa aggccaagca 660

gaaaacagaa aagtttagga ctcctgtgcc agaagtttct agctcgctat ccaagttatc 720

ccttgtcaac tgagaaaact accatctccc tagatgaagt tgctgtcagt cttggtgtgg 780

aaaggagacg catctatgac attgtaaatg tgctggagtc gctgcatctg gtcagccggg 840

tggctaagaa tcagtatggc tggcatggac ggcacagcct gccaaaaacc ctgaggaacc 900

tccagagact aggagaggag cagaaatatg aagagcaaat ggcctacctc caacagaaag 960

agctggacct gatagattat aaatttggag aacgtaaaaa agatggtgat ccagattccc 1020

aggaacaaca gttactggat ttctctgaac ccgactgtcc ctcttcatct gcaaacagta 1080

gaaaagacaa gtctctgaga attatgagcc agaagtttgt catgctgttc ctcgtctcca 1140

aaaccaagat tgtcactctg gatgtggctg ccaaaatact gatagaagaa agccaagatg 1200

ccccagacca tagtaaattt aaaacaaagg tacgacgcct ctatgacata gccaatgttc 1260

tgaccagctt ggctctgata aagaaagtgc atgtaacaga agagcgaggt cgtaaaccag 1320

ccttcaagtg gatcgggcct gtggacttca gctcaagtga tgaagaactg gtggatgttt 1380

ctgcatctgt cttaccagaa ttgaaaagag aaacatatgg ccagattcaa gtctgtgcaa 1440

aacagaagct ggctcgccat ggttctttta acacagttca ggcttctgag aggatccaga 1500

ggaaagtgaa ctcagaaccg agcagcccgt acagagaaga acaaggatca ggtggctact 1560

ctttagaaat tggaagcctg gcagctgtct atagacagaa aatagaagac aattcacagg 1620

gaaaagcctt tgccagtaag agagtggtgc ctccatcaag cagcttggac cctgttgctc 1680

ctttccctgt cctctctgtt gacccagaat attgtgttaa tcctttagcc cacccagtat 1740

tttctgttgc tcagacggac ctgcaggcat tctccatgca gaacggtctg aatggacaag 1800

tggatgtctc acttgcttct gcagcctctg ctgtggagag cctgaagcca gcactccttg 1860

ctggccagcc tctagtgtat gtgccctctg cctcactgtt catgctgtat ggaagtctgc 1920

aggagggacc agcgtcaggg tcagggtcag agagggatga cagaagctca gaagccccag 1980

ccacagtaga gctgtcatct gcaccctcag ctcagaagcg cctctgtgag gagaggaaac 2040

ctcaggagga ggatgagcca gccactaaaa ggcaaagtag ggaatatgaa gacggcccgc 2100

tgtcgcttgt catgcccaag aaaccctcag attccacaga ccttgcctct cccaagacta 2160

tgggtaacag ggcatctata cccctcaaag acattcatgt gaatggccaa ctccctgctg 2220

cagaagagat ttcaggaaag gcaacagcaa actctcttgt ttcttctgag tggggaaatc 2280

cttcaagaaa tacagatgtt gaaaagcctt caaaagaaaa tgaaagcacc aaagagcctt 2340

ctttgctaca atatctttgt gtgcagtctc ctgcaggatt aaatggtttc aatgtacttt 2400

tatctggcag tcaaaccccc cctactgtgg gcccgtcctc aggtcagctg ccgtctttca 2460

gtgtcccttg catggtctta ccatctccac ctctgggccc ttttcctgtt ctctattctc 2520

ctgcaatgcc gggcccggtt tcttctactc ttggtgctct cccaaacaca ggacctgtga 2580

atttcagctt gcctggcctt ggatcaatag cccagcttct cgtcggcccc acagctgtgg 2640

ttaatccaaa gtcgtccaca ctcccttctg cagaccctca gcttcagagt cagccctcac 2700

taaacctaag tccagtgatg tcaaggtcac acagtgtcgt ccaacaacct gagtcccccg 2760

tttacgtggg acatccagtc tcagtagtaa aattacatca gtcaccagtt ccagtgaccc 2820

ccaagagcat ccaacgcaca catcgtgaga cgtttttcaa gacacccggc agccttggag 2880

accctgtcct gaagagaaga gaaaggaacc agtcacgaaa caccagctcg gcccagagga 2940

gactagaaat ccccagcggc ggcgctgact aacctgccgc tttgccaggt gggggtggga 3000

tcaaacgccc tgagagtccc ggatgtccga ggcgggatgc aaaccatccc gtcctgagca 3060

cgggtccttc ctctctcttt catccacact tctgttaact tcccaccacc atcaatcatc 3120

tgatttcctg aaagtaatta attgtgcatt taataccagt tagagttccg actctgcatg 3180

gtgtcacagt gaaagcgccg actgacttat ggttttgatt caagaatcgt cttattctgg 3240

aagtagatct gaataggcta ccggagcctt gtttttctaa aggggggcgc tgtctagcac 3300

ttaactaggg taagcattct taacatgtat ttccacttgc cctgagtaaa tctgtggtga 3360

gagaagcttc ctttctgcag tttaaaaaag ctactgcttc cttaggcttc atcaggaagc 3420

caccttcagt tgtgaatcct atggtgttat ttattttgtt cctgaaatgg gatttagtgc 3480

aaaaagttta caactacagt ctttaacaca tttttttcag ggtatgacga cttgaatgtt 3540

tatactttta ttctataatt tgccctgcac ttattttaca acctagtaat aatgtggata 3600

aatgtatcta catgacacat gtcaagacca aaataactgt gaatgacaca ccttgctgta 3660

aatgaactgt gctaaccctg actgtgggct tgagaacaaa gatgaactct agaactctag 3720

cagcctaact gctgcttctc aaataactgt gtgaacagtg agatattact gtttgtttct 3780

aaaaatccta ctgtgcccag tttccttcac tacatgccct gcatttttta tttaaatatt 3840

tagctgtagc gccatcagat atggatgcct tctaacaatt gctgtttgta aaataaatca 3900

ggatggtaga aagtgattat atggaaaatt ggaacctgga tgagaccttt tcgttgaatt 3960

ctgaagagta atgatgtgaa aattgataca gggcaagaga tgattctttt gtttttcttc 4020

tacttcatgt ccagaagagt aagagggaaa atggacatat gtttcatatc caagggtatt 4080

caaactgtag ttagttggta cctctgaaaa atgagaatgg tgagcgcacg ggttggttgt 4140

tctagcatga atacaattct ggaaactgtt atgcaatttc ccttttttaa cccacattac 4200

tttaggggtg cattaagtcg ccaaactata ctagttcttt gtattcctag acttgctgat 4260

atttacctct ctcttgtctc ttcagagtaa atggttccct tctttccttc ctactttcct 4320

tcattctctc ttccttccct ccttcctact tcttttcttc cttcctcttc ctctcttaaa 4380

actatcttag atgtagaatc ctggtgtagg gttttatttt atttttattt tttgacccaa 4440

taaaatgtta tatgaaagaa tgaaaatatt aatttaagag actctgggag tctgaataaa 4500

gtagctttat attaactaca ggataatatt agccttatta cccccacaag attttttaaa 4560

acttgaggta ggtagctaca ttaaataaat ttgctactta tataaaaatt tttatcaaca 4620

ctaaactttt aaagtttaca agtttttttt ttctttttta cagtcttcta tagagttagg 4680

ttaaaaatgt ggttctaacc atcaacaatt gcatggttaa atgaccctga actaaaactg 4740

atgggttccc tatcaaaaca aataaaaata tacctttttc aggtttcaat ctgtgcaggg 4800

tatatgcatg ttaattctac catgcttaag aacttccaca aaatatttca tggagaggtc 4860

tgcatttaga cggaaacaga aattgctttt cccctcactg ttcctgaatg ctctatactt 4920

gttttaacat ttttgctatc tttttttatt attctgatca tgatatgacc atttaacctc 4980

agaattcata attcctgagg ggtgttaaga agcagtccca ttggtgagga tattatgact 5040

tggtgaccat tcttaggagt agaaaaccaa ggacaattgc ttctgtattc agtatccact 5100

tcttaatgtg gctttatatg taaaaataat aatgcagtgg ttgtttctgt caggaaaata 5160

aatcttacag aacaactggt ggaattgaag ctgctgcgct agacttggat attttgggta 5220

gtgaagaagc aatggcaatc ttgagtctat tattgtataa tttagtaaaa gaaaaaaata 5280

atcgttggtg gtcctactaa gagaatgcag cttttttgag ttgtcacaga ggctgtgtgt 5340

gccctacact gaccagggtt tgtaaaaccc tttcattctg gtacaagagt cgggggtata 5400

acttttatac ttgaatctac ctaccaagtt tacatttctc aattcctttt tgtaaggtgc 5460

tatttctgta tttaaataac tttcttttaa cgtaaagctg ctttctgctt atcttattgc 5520

actgctagtt gtatgtaggt attaatttta ttgctgctta ctgcttttgt tttcttatta 5580

tttagctctg ctctttttcc taatggctat attatctata gctatttact tgtaactgta 5640

ctacatgtaa actgattttt tgttctgatt ttttttctaa tatttttagg aaaatattaa 5700

gctttataaa atagcaataa aaaataattc atttaagaag 5740

<210> 26

<211> 699

<212> DNA

<213>mankind CPS1 gene

<400> 26

gctcttttaa aatagttgct ttcttaggaa atgtagttgc tttcttaacc tcatcaaatt 60

catgaagatt tagccgaggc ccatgctacc cattatcttc taaatggcct tcagaaatga 120

tggagaaact gacacgttat gagtatttcc tgccctacat ggattttgaa cttgaattcc 180

cagcctcgct tgcatctaga cacaggcacg tgacccaagc cccaccagtc agacaaaccc 240

aattgaaact ttagttcaaa agcatccagt atgagggagc agtgaagttt gcattttcct 300

gatgaaaatg ttggaagagc cacaaatcat caaaatgacg aggattttga cagctttcaa 360

agtggtgagg acactgaaga ctggttttgg ctttaccaat gtgactgcac accaaaaatg 420

gaaattttca agacctggca tcaggctcct ttctgtcaag gcacagacag cacacattgt 480

cctggaagat ggaactaaga tgaaaggtta ctcctttggc catccatcct ctgttgctgg 540

tgaagtggtt tttaatactg gcctgggagg gtacccagaa gctattactg accctgccta 600

caaaggacag attctcacaa tggccaaccc tattattggg aatggtggag ctcctgatac 660

tactgctctg gatgaactgg gacttagcaa atatttgga 699

<210> 27

<211> 6609

<212> DNA

<213>mankind ABCA3 gene

<400> 27

gtccgccagt agcgggttgc aggcgcaccc tcccctccag ggcggccacg cagctgtcag 60

tgccgccgcc actgcgaggc tggagcggag cccgggtggc cgagggaggg gaccccgcga 120

gagggccgcg cgccggccgc cgccgccccg gcgcccaggc tcggtgctgg agagtcatgc 180

ctgtgagccc tgggcacctc ctgatgtcct gcgaggtcac ggtgttccca aacctcaggg 240

ttgccctgcc ccactccaga ggctctcagg ccccaccccg gagccctctg tgcggagccg 300

cctcctcctg gccagttccc cagtagtcct gaagggagac ctgctgtgtg gagcctcttc 360

tgggacccag ccatgagtgt ggagctgagc aactgaacct gaaactcttc cactgtgagt 420

caaggaggct tttccgcaca tgaaggacgc tgagcgggaa ggactcctct ctgcctgcag 480

ttgtagcgag tggaccagca ccaggggctc tctagactgc ccctcctcca tcgccttccc 540

tgcctctcca ggacagagca gccacgtctg cacacctcgc cctctttaca ctcagttttc 600

agagcacgtt tctcctattt cctgcgggtt gcagcgccta cttgaactta ctcagaccac 660

ctacttctct agcagcactg ggcgtccctt tcagcaagac gatggctgtg ctcaggcagc 720

tggcgctcct cctctggaag aactacaccc tgcagaagcg gaaggtcctg gtgacggtcc 780

tggaactctt cctgccattg ctgttttctg ggatcctcat ctggctccgc ttgaagattc 840

agtcggaaaa tgtgcccaac gccaccatct acccgggcca gtccatccag gagctgcctc 900

tgttcttcac cttccctccg ccaggagaca cctgggagct tgcctacatc ccttctcaca 960

gtgacgctgc caagaccgtc actgagacag tgcgcagggc acttgtgatc aacatgcgag 1020

tgcgcggctt tccctccgag aaggactttg aggactacat taggtacgac aactgctcgt 1080

ccagcgtgct ggccgccgtg gtcttcgagc accccttcaa ccacagcaag gagcccctgc 1140

cgctggcggt gaaatatcac ctacggttca gttacacacg gagaaattac atgtggaccc 1200

aaacaggctc ctttttcctg aaagagacag aaggctggca cactacttcc cttttcccgc 1260

ttttcccaaa cccaggacca agggaaccta catcccctga tggcggagaa cctgggtaca 1320

tccgggaagg cttcctggcc gtgcagcatg ctgtggaccg ggccatcatg gagtaccatg 1380

ccgatgccgc cacacgccag ctgttccaga gactgacggt gaccatcaag aggttcccgt 1440

acccgccgtt catcgcagac cccttcctcg tggccatcca gtaccagctg cccctgctgc 1500

tgctgctcag cttcacctac accgcgctca ccattgcccg tgctgtcgtg caggagaagg 1560

aaaggaggct gaaggagtac atgcgcatga tggggctcag cagctggctg cactggagtg 1620

cctggttcct cttgttcttc ctcttcctcc tcatcgccgc ctccttcatg accctgctct 1680

tctgtgtcaa ggtgaagcca aatgtagccg tgctgtcccg cagcgacccc tccctggtgc 1740

tcgccttcct gctgtgcttc gccatctcta ccatctcctt cagcttcatg gtcagcacct 1800

tcttcagcaa agccaacatg gcagcagcct tcggaggctt cctctacttc ttcacctaca 1860

tcccctactt cttcgtggcc cctcggtaca actggatgac tctgagccag aagctctgct 1920

cctgcctcct gtctaatgtc gccatggcaa tgggagccca gctcattggg aaatttgagg 1980

cgaaaggcat gggcatccag tggcgagacc tcctgagtcc cgtcaacgtg gacgacgact 2040

tctgcttcgg gcaggtgctg gggatgctgc tgctggactc tgtgctctat ggcctggtga 2100

cctggtacat ggaggccgtc ttcccagggc agttcggcgt gcctcagccc tggtacttct 2160

tcatcatgcc ctcctattgg tgtgggaagc caagggcggt tgcagggaag gaggaagaag 2220

acagtgaccc cgagaaagca ctcagaaacg agtactttga agccgagcca gaggacctgg 2280

tggcggggat caagatcaag cacctgtcca aggtgttcag ggtgggaaat aaggacaggg 2340

cggccgtcag agacctgaac ctcaacctgt acgagggaca gatcaccgtc ctgctgggcc 2400

acaacggtgc cgggaagacc accaccctct ccatgctcac aggtctcttt ccccccacca 2460

gtggacgggc atacatcagc gggtatgaaa tttcccagga catggttcag atccggaaga 2520

gcctgggcct gtgcccgcag cacgacatcc tgtttgacaa cttgacagtc gcagagcacc 2580

tttatttcta cgcccagctg aagggcctgt cacgtcagaa gtgccctgaa gaagtcaagc 2640

agatgctgca catcatcggc ctggaggaca agtggaactc acggagccgc ttcctgagcg 2700

ggggcatgag gcgcaagctc tccatcggca tcgccctcat cgcaggctcc aaggtgctga 2760

tactggacga gcccacctcg ggcatggacg ccatctccag gagggccatc tgggatcttc 2820

ttcagcggca gaaaagtgac cgcaccatcg tgctgaccac ccacttcatg gacgaggctg 2880

acctgctggg agaccgcatc gccatcatgg ccaaggggga gctgcagtgc tgcgggtcct 2940

cgctgttcct caagcagaaa tacggtgccg gctatcacat gacgctggtg aaggagccgc 3000

actgcaaccc ggaagacatc tcccagctgg tccaccacca cgtgcccaac gccacgctgg 3060

agagcagcgc tggggccgag ctgtctttca tccttcccag agagagcacg cacaggtttg 3120

aaggtctctt tgctaaactg gagaagaagc agaaagagct gggcattgcc agctttgggg 3180

catccatcac caccatggag gaagtcttcc ttcgggtcgg gaagctggtg gacagcagta 3240

tggacatcca ggccatccag ctccctgccc tgcagtacca gcacgagagg cgcgccagcg 3300

actgggctgt ggacagcaac ctctgtgggg ccatggaccc ctccgacggc attggagccc 3360

tcatcgagga ggagcgcacc gctgtcaagc tcaacactgg gctcgccctg cactgccagc 3420

aattctgggc catgttcctg aagaaggccg catacagctg gcgcgagtgg aaaatggtgg 3480

cggcacaggt cctggtgcct ctgacctgcg tcaccctggc cctcctggcc atcaactact 3540

cctcggagct cttcgacgac cccatgctga ggctgacctt gggcgagtac ggcagaaccg 3600

tcgtgccctt ctcagttccc gggacctccc agctgggtca gcagctgtca gagcatctga 3660

aagacgcact gcaggctgag ggacaggagc cccgcgaggt gctcggtgac ctggaggagt 3720

tcttgatctt cagggcttct gtggaggggg gcggctttaa tgagcggtgc cttgtggcag 3780

cgtccttcag agatgtggga gagcgcacgg tcgtcaacgc cttgttcaac aaccaggcgt 3840

accactctcc agccactgcc ctggccgtcg tggacaacct tctgttcaag ctgctgtgcg 3900

ggcctcacgc ctccattgtg gtctccaact tcccccagcc ccggagcgcc ctgcaggctg 3960

ccaaggacca gtttaacgag ggccggaagg gattcgacat tgccctcaac ctgctcttcg 4020

ccatggcatt cttggccagc acgttctcca tcctggcggt cagcgagagg gccgtgcagg 4080

ccaagcatgt gcagtttgtg agtggagtcc acgtggccag tttctggctc tctgctctgc 4140

tgtgggacct catctccttc ctcatcccca gtctgctgct gctggtggtg tttaaggcct 4200

tcgacgtgcg tgccttcacg cgggacggcc acatggctga caccctgctg ctgctcctgc 4260

tctacggctg ggccatcatc cccctcatgt acctgatgaa cttcttcttc ttgggggcgg 4320

ccactgccta cacgaggctg accatcttca acatcctgtc aggcatcgcc accttcctga 4380

tggtcaccat catgcgcatc ccagctgtaa aactggaaga actttccaaa accctggatc 4440

acgtgttcct ggtgctgccc aaccactgtc tggggatggc agtcagcagt ttctacgaga 4500

actacgagac gcggaggtac tgcacctcct ccgaggtcgc cgcccactac tgcaagaaat 4560

ataacatcca gtaccaggag aacttctatg cctggagcgc cccgggggtc ggccggtttg 4620

tggcctccat ggccgcctca gggtgcgcct acctcatcct gctcttcctc atcgagacca 4680

acctgcttca gagactcagg ggcatcctct gcgccctccg gaggaggcgg acactgacag 4740

aattatacac ccggatgcct gtgcttcctg aggaccaaga tgtagcggac gagaggaccc 4800

gcatcctggc ccccagtccg gactccctgc tccacacacc tctgattatc aaggagctct 4860

ccaaggtgta cgagcagcgg gtgcccctcc tggccgtgga caggctctcc ctcgcggtgc 4920

agaaagggga gtgcttcggc ctgctgggct tcaatggagc cgggaagacc acgactttca 4980

aaatgctgac cggggaggag agcctcactt ctggggatgc ctttgtcggg ggtcacagaa 5040

tcagctctga tgtcggaaag gtgcggcagc ggatcggcta ctgcccgcag tttgatgcct 5100

tgctggacca catgacaggc cgggagatgc tggtcatgta cgctcggctc cggggcatcc 5160

ctgagcgcca catcggggcc tgcgtggaga acactctgcg gggcctgctg ctggagccac 5220

atgccaacaa gctggtcagg acgtacagtg gtggtaacaa gcggaagctg agcaccggca 5280

tcgccctgat cggagagcct gctgtcatct tcctggacga gccgtccact ggcatggacc 5340

ccgtggcccg gcgcctgctt tgggacaccg tggcacgagc ccgagagtct ggcaaggcca 5400

tcatcatcac ctcccacagc atggaggagt gtgaggccct gtgcacccgg ctggccatca 5460

tggtgcaggg gcagttcaag tgcctgggca gcccccagca cctcaagagc aagttcggca 5520

gcggctactc cctgcgggcc aaggtgcaga gtgaagggca acaggaggcg ctggaggagt 5580

tcaaggcctt cgtggacctg acctttccag gcagcgtcct ggaagatgag caccaaggca 5640

tggtccatta ccacctgccg ggccgtgacc tcagctgggc gaaggttttc ggtattctgg 5700

agaaagccaa ggaaaagtac ggcgtggacg actactccgt gagccagatc tcgctggaac 5760

aggtcttcct gagcttcgcc cacctgcagc cgcccaccgc agaggagggg cgatgagggg 5820

tggcggctgt ctcgccatca ggcagggaca ggacgggcaa gcagggccca tcttacatcc 5880

tctctctcca agtttatctc atcctttatt tttaatcact tttttctatg atggatatga 5940

aaaattcaag gcagtatgca cagaatggac gagtgcagcc cagccctcat gcccaggatc 6000

agcatgcgca tctccatgtc tgcatactct ggagttcact ttcccagagc tggggcaggc 6060

cgggcagtct gcgggcaagc tccggggtct ctgggtggag agctgaccca ggaagggctg 6120

cagctgagct gggggttgaa tttctccagg cactccctgg agagaggacc cagtgacttg 6180

tccaagttta cacacgacac taatctcccc tggggaggaa gcgggaagcc agccaggttg 6240

aactgtagcg aggcccccag gccgccagga atggaccatg cagatcactg tcagtggagg 6300

gaagctgctg actgtgatta ggtgctgggg tcttagcgtc cagcgcagcc cgggggcatc 6360

ctggaggctc tgctccttag ggcatggtag tcaccgcgaa gccgggcacc gtcccacagc 6420

atctcctaga agcagccggc acaggaggga aggtggccag gctcgaagca gtctctgttt 6480

ccagcactgc accctcagga agtcgcccgc cccaggacac gcagggacca ccctaagggc 6540

tgggtggctg tctcaaggac acattgaata cgttgtgacc atccagaaaa taaatgctga 6600

ggggacaca 6609

<210> 28

<211> 6656

<212> DNA

<213>mankind TICRR gene

<400> 28

atggcatgct gtcacaaagt aatgctgctg ctggacaccg cgggcggcgc cgcccgccac 60

agccgggtcc ggcgggccgc cctgcgcctc ctcacctatc tgagttgccg attcggcctg 120

gccagggtcc actgggcctt caagttcttt gactcgcagg gggcgcggag ccggccgtcc 180

cgcgtgtctg acttccgcga gctggggtcc cgctcgtggg aggactttga ggaggagctg 240

gaggccaggc tcgaggatcg cgcccacctg cccggcccgg cgcccagggc cacccacacg 300

cacggcgccc tgatggagac gctgctagac taccagtggg accggcccga gatcacgtcg 360

cccacgaagc cgatcctgcg gagcagcggg aggagactgc tggacgtgga gagcgaggcc 420

aaggaggccg aggccgcgct cgggggcttg gtgaacgccg tcttcctcct ggccccctgt 480

ccgcactcgc agagggagct gctgcagttc gtgtctgggt gcgaggccca ggcccagcgc 540

ctgccgccca cccctaagca ggtgatggag aagttgttgc ccaagagagt ccgggaagtc 600

atggtcgccc gaaaaatcac cttctactgg gtggatacca ccgaatggtc taagttgtgg 660

gaatccccag accaccttgg atactggact gtttgtgaac tgctccacca cggaggtggc 720

actgtcttgc catctgaatc tttcagctgg gattttgctc aagctgggga aatgctgctc 780

aggagtggaa taaagctgtc aagtgaacct catctttctc cgtggatttc aatgctgcca 840

actgatgcca ctttaaaccg tttgctctac aattctcctg agtatgaggc ctcgtttcca 900

cgaatggaag gaatgttatt tctccctgtt gaaggcaaag agattcaaga aacatggaca 960

gtcaccctag agcccttggc catgcatcag agacattttc agaaaccagt cagaattttt 1020

ctaaaaggct cagtggccca gtggtctctc ccaacgagca gcactttggg cactgacagc 1080

tggatgctag gaagtccaga ggagagcaca gcaactcaaa ggctgttatt tcagcagttg 1140

gtaagcaggc tgactgctga agagttacac ctggttgctg atgtggaccc tggtgaaggc 1200

cggcccccca tcactggagt tatttcccca ctctctgcca gtgctatgat cctcactgtg 1260

tgccgcacca aggaggctga atttcaacga catgttctcc aaacagctgt ggctgacagc 1320

ccccgggaca cagcttccct tttctcagat gttgtggata gtatattgaa tcagactcat 1380

gattcgcttg cagatactgc ttctgctgct tctcctgttc cagagtgggc ccagcaggag 1440

cttggccaca ccactccctg gagtccagct gttgtggaaa agtggtttcc tttctgtaac 1500

atcagtggtg ccagttccga tttgatggag tcatttgggt tactacaggc tgcctcagct 1560

aataaggaag agtcttccaa aactgaaggc gaattaatac attgccttgc cgagctctac 1620

cagagaaaat ctcgtgaaga atccactata gctcatcaag aagacagcaa aaagaaacga 1680

ggggtccctc gtactccagt gagacagaag atgaatacca tgtgccgttc cttaaagatg 1740

ttgaatgtcg caaggctgaa tgtgaaggcc cagaagttac atccagatgg cagtccggat 1800

gtggctgggg agaaaggaat ccaaaagata cctagtggga gaacagtgga taaattggaa 1860

gacagaggaa gaacactaag aagttctaaa cctaaagatt ttaaaactga ggaagagctg 1920

ctatcatata tacgtgaaaa ttaccaaaag actgtggcca caggagaaat catgttgtat 1980

gcatgtgctc gaaacatgat ctcaaccgtt aaaatgttcc taaaatcaaa aggcaccaag 2040

gaattagaag tgaactgcct gaatcaagta aaaagtagtc tcttaaaaac tagtaaaagt 2100

cttcgacaga atctaggaaa aaaactggat aaggaagaca aagttagaga gtgccagctt 2160

caggtatttc ttcgtttgga gatgtgtctg caatgccctt caataaatga aagtacagat 2220

gatatggaac aagtagtgga ggaggtgaca gatttgctgc gcatggtgtg tttaactgag 2280

gattcagcgt acctagcaga gtttctggag gaaattttga gattgtatat tgactctatc 2340

ccaaagacac ttggaaatct ttacaacagc ctagggtttg tgattcctca gaagctggct 2400

ggtgtccttc ctacagattt tttcagtgat gactccatga cacaagagaa caaatcacca 2460

cttctttctg tgcctttttt gtcaagtgct cgtagatcag tgtcaggcag ccctgaatct 2520

gatgaactgc aggaacttcg taccagatca gccaagaaga gaaggaaaaa tgcattaata 2580

agacataaaa gcattgctga ggtttcacag aatcttcgac aaattgaaat tcctaaagtg 2640

tcaaagagag ctacgaaaaa agagaactct caccctgctc ctcagcagcc ttcccagcca 2700

gtgaaagata cagtgcaaga agtgaccaaa gttcgaagaa atcttttcaa ccaggaattg 2760

ctttcccctt caaagagatc actaaagcgg gggttgccta gaagccattc tgtgtcagct 2820

gtggatggtc tagaggataa acttgacaac ttcaagaaga acaaaggtta tcacaaactg 2880

ctgactaaga gtgtggccga gactccagtg cataagcaga tctccaaaag gctgctgcac 2940

agacaaatca agggcaggtc ctctgatcct ggtcctgata ttggtgttgt tgaagagtcc 3000

cctgaaaaag gagatgaaat aagtctgaga cgaagtcctc gaatcaagca gttgtcattt 3060

agcaggacac attctgcctc cttctattct gtgtctcagc cgaagtctcg aagtgtgcaa 3120

agagtccact ctttccagca agataagtca gaccaaagag aaaattctcc agtccaaagt 3180

attcggtctc ccaagagtct tctttttggg gcaatgtctg agatgatcag cccctcagaa 3240

aagggttcag ctcgaatgaa aaagcgttca agaaacactt tggattcgga ggtacctgca 3300

gcttaccaga ctcccaagaa gagtcaccag aaatctctga gcttttctaa aactacacca 3360

agaaggatct ctcatacacc acaaactccg ttgtatactc cagaaaggct gcagaagtcc 3420

cctgcaaaaa tgacccctac aaagcaggca gcttttaagg agtccttaaa agactcctcc 3480

tcacccggcc atgactcacc attggattca aaaatcactc ctcaaaaacg acatacccag 3540

gcaggagaag gtacctctct tgaaacgaag acaccaagaa ctcctaagag gcaaggtact 3600

cagccgcctg ggtttttgcc aaactgtact tggccacatt cagtgaattc cagtccagaa 3660

agcccctcct gtccagcccc tccaacttca tcgactgccc agcccaggag agagtgtctc 3720

actcccatca gagaccctct cagaacacct ccgagagcag cagccttcat gggcacgcct 3780

cagaatcaaa cacaccaaca gccccatgtc ctcagagctg ctcgggcaga ggaaccagcc 3840

cagaaactaa aggataaagc tatcaaaact ccaaaaagac cagggaattc aactgtgact 3900

tcttccccac ctgttacgcc aaagaaactg tttacctctc ctttatgtga tgtctccaag 3960

aagagtccat ttaggaaatc taaaatagag tgtccttccc caggagaact ggatcagaaa 4020

gagccccaga tgtcacccag cgtagctgca tctctctcct gccctgttcc ctcaactccc 4080

cctgaactct cacagagagc tacattggac accgtccctc ctccaccccc ttctaaagtt 4140

gggaaacggt gtagaaagac ctctgatccc agaaggagca tcgtggagtg tcagcctgat 4200

gcctccgcta ctcctggggt tggcacagct gacagcccag ctgcccccac agactctaga 4260

gatgaccaga agggactgag cctctctcct cagtctcctc ctgaaagacg gggctaccca 4320

ggccctggtc tcaggagtga ttggcatgca tcctctcctc tgctcattac aagtgacaca 4380

gagcatgtca ctctcctcag tgaagccgaa caccatggca ttggtgactt gaaaagtaac 4440

gtcttatcag tggaagaggg tgaggggcta aggacagcag atgctgagaa gtcttctctg 4500

tctcaccctg ggattccccc atctcctcct tcctgtgggc ctggctctcc tctgatgcct 4560

tcccgtgacg tgcactgtac cacagatggg agacagtgcc aggcttcggc acaactagac 4620

aacctgccag catcagcttg gcattccaca gactctgcca gcccacagac ctatgaggtt 4680

gagctggaga tgcaagcttc tggccttccc aaacttcgaa ttaagaagat agaccccagc 4740

tcttcattag aggctgagcc cctcagcaag gaggagagct ctctgggaga agagagcttc 4800

ctccctgctc tcagcatgcc cagggccagc aggtccttaa gcaaacctga acccacctat 4860

gtgtcacccc cctgcccccg cctctcccac agcacacctg gcaagagcag ggggcaaacc 4920

tacatctgcc aggcctgtac ccccacccac ggcccttcta gtaccccctc tccatttcaa 4980

acagatgggg ttccttggac accatccccc aagcacagtg ggaagacaac tccagacata 5040

attaaagact ggcccaggag gaagagggcg gtgggctgtg gcgccggctc ctcttccggg 5100

aggggcgagg tcggtgcaga ccttcctggg agcctgtcac tgcttgagtc agagggcaag 5160

gaccacggcc ttgaactcag catccacagg acgcccatct tggaggattt tgagctcgag 5220

ggagtgtgcc agctcccaga ccagtcgcct cccaggaaca gcatgcctaa ggccgaggaa 5280

gcctcttcct ggggacagtt tgggttgagt tccaggaaga gagtcctgtt ggccaaggaa 5340

gaagctgacc gtggagccaa aaggatctgt gacctgagag aagattcaga agttagtaag 5400

agtaaagagg ggtctccaag ttggagtgca tggcagctac cctccacggg agacgaagag 5460

gtgtttgttt ccggctccac cccacctccc agctgtgccg tgcggagctg cctctctgcc 5520

agtgccctcc aggctctgac ccagtctccg ctgctgttcc aggggaaaac accttcctct 5580

cagagcaaag accccagaga tgaggatgtg gatgttcttc cctccactgt agaagactct 5640

cctttcagtc gcgctttctc caggaggcgc cccatcagca gaacttatac acggaagaag 5700

ctcatgggaa cctggctgga ggacttatag ccacaaacat tactgagccc aaaagatcaa 5760

ggagtcagcc aggaccctgt ggacataaag aagttggatg cctggtccca agcctctttt 5820

gccatggtca gtgttcagat tgccattaga atgccttagg gttttctaat tccccttatg 5880

gatccaatcc atctcctggc cctgcccctt gttggggaag ttgcaggagg agaggtggat 5940

ggcaatgtga ttggtgctat aactcaggca gcctgggagt caggaaccca gacaaggaat 6000

cccattccag cctcacccca accatgacct tggcaagtca gggggccact ctgcctcatt 6060

tatgcaaatg gagaaaggcg ccctccctgg ggtcccttga gctgctgtaa ggctgggctg 6120

ctgcgacaca ggcagcgctt tgtaaactgt gaagccatat acgtgaaact gaagagtgca 6180

ttgggcagtg gaagctattt tttgccttcc ctgtgtaaca gtaaaatcat ctctagtgac 6240

tgagcactca gtacattttt gtttaatgtt gggcctgagg ttaactgtga ccatggtcca 6300

gcttgagtgg cttctggagc agccacattt tcaaggactg tccaagagcc agccagttca 6360

gggctcaggc ctcacccatt gcccactcct ggggagacca tcacctggct catcgtttcc 6420

accaagagtg ccccacagga gtgccccaca gacccgctgg accagcctgc tgcgggtcct 6480

ggccaggggt ctggctaacg gtgagggctg actctgaact gtctctcagt ctccagaaag 6540

tgttcaagcc tgttgtgttc ccaaatctga ttcctcctat tgtcttgtaa atcaaactct 6600

aagtgaaaac ttcccatttg tcccttcaaa gatttttttt tattaaatgg tttttt 6656

<210> 29

<211> 689

<212> DNA

<213>mankind CDKN3 gene

<400> 29

ctaggaacca gtcagcgatt agaggccgag tcttcggcca cccaaaggcg gagtaagaaa 60

ccagaagcgg atctgattgg ttgctggaag acgccgcgcc cacctcacag aaggacgaac 120

cagtgagcta agctgcgggg cgcgggctcg gccggggcac cggtgagtcg ccggcgctgc 180

agagggaggc ggcactggtc tcgacgtggg gcggccagcg atgaagccgg ctatctttgt 240

cacgagtgaa ttgttctcag tttctcggtt tatgtgctct tccaggttgt aaatttaaag 300

atgttagaag aaatgtccaa aaagatacag aagaactaaa gagctgtggt atacaagaca 360

tatttgtttt ctgcaccaga ggggaactgt caaaatatag agtcccaaac cttctggatc 420

tctaccagca atgtggaatt atcacccatc atcatccaat cgcagatgga gggactcctg 480

acatagccag ctgctgtgaa ataatggaag agcttacaac ctgccttaaa aattaccgaa 540

aaaccttaat acactgctat ggaggacttg ggagatcttg tcttgtagct gcttgtctcc 600

tactatacct gtctgacaca atatcaccag agcaagccat agacagcctg cgagacctaa 660

gaggatccgg ggcaatacag accatcaag 689

<210> 30

<211> 3161

<212> DNA

<213>mankind VIPR1 gene

<400> 30

attttcccag cttactagtg gaaaagcagc ttctttccca cagttccagc aaaagcccca 60

gggctgatgc tcattggccc aggttggggc atgtgaccat tggcaagact cccattggcc 120

aggcctgggg caggtgctca ctactggagc cagagcggag tataagccct acccaatcac 180

tgaactacgt gggtcaagag taaaggagct gtggcttccc cccaatcaga agggcactgg 240

atgatggatg aactctgtgt ggtgcgtctt cacactgaca ccaggaggat aaggaagtga 300

ctccctcagc tgagtggctg gcatgtgcca ggctagagca gcttggctat ggcagaacat 360

ccagccccac cgtgtggacg gcagagatga ggaaactggt gtggggttgc cggggccagc 420

aaccactttt ctaaagccaa agacgcagaa ggtgctgcca gtccaacgct tcctccatgg 480

aggccttcac ccacccacac ccatctaaga gttcccaaga cctgtttttt ctggctattc 540

ctagacagcc atcaccccca ctcacatgtc ttccacaagg gcggccaggc ggccaggctg 600

caggaggagt gtgactatgt gcagatgatc gaggtgcagc acaagcagtg cctggaggag 660

gcccagctgg agaatgagac aataggctgc agcaagatgt gggacaacct cacctgctgg 720

ccagccaccc ctcggggcca ggtagttgtc ttggcctgtc ccctcatctt caagctcttc 780

tcctccattc aaggccgcaa tgtaagccgc agctgcaccg acgaaggctg gacgcacctg 840

gagcctggcc cgtaccccat tgcctgtggt ttggatgaca aggcagcgag tttggatgag 900

cagcagacca tgttctacgg ttctgtgaag accggctaca ccattggcta cggcctgtcc 960

ctcgccaccc ttctggtcgc cacagctatc ctgagcctgt tcaggaagct ccactgcacg 1020

cggaactaca tccacatgca cctcttcata tccttcatcc tgagggctgc cgctgtcttc 1080

atcaaagact tggccctctt cgacagcggg gagtcggacc agtgctccga gggctcggtg 1140

ggctgtaagg cagccatggt ctttttccaa tattgtgtca tggctaactt cttctggctg 1200

ctggtggagg gcctctacct gtacaccctg cttgccgtct ccttcttctc tgagcggaag 1260

tacttctggg ggtacatact catcggctgg ggggtaccca gcacattcac catggtgtgg 1320

accatcgcca ggatccattt tgaggattat gggtgctggg acaccatcaa ctcctcactg 1380

tggtggatca taaagggccc catcctcacc tccatcttgg taaacttcat cctgtttatt 1440

tgcatcatcc gaatcctgct tcagaaactg cggcccccag atatcaggaa gagtgacagc 1500

agtccatact caaggctagc caggtccaca ctcctgctga tccccctgtt tggagtacac 1560

tacatcatgt tcgccttctt tccggacaat tttaagcctg aagtgaagat ggtctttgag 1620

ctcgtcgtgg ggtctttcca gggttttgtg gtggctatcc tctactgctt cctcaatggt 1680

gaggtgcagg cggagctgag gcggaagtgg cggcgctggc acctgcaggg cgtcctgggc 1740

tggaacccca aataccggca cccgtcggga ggcagcaacg gcgccacgtg cagcacgcag 1800

gtttccatgc tgacccgcgt cagcccaggt gcccgccgct cctccagctt ccaagccgaa 1860

gtctccctgg tctgaccacc aggatcccag gggcccaagg cggcccctcc cgccccttcc 1920

cactcacccc ggcagacgcc ggggacagag gcctgcccgg gcgcggccag ccccggccct 1980

gggctcggag gctgcccccg gccccctggt ctctggtccg gacactccta gagaacgcag 2040

ccctagagcc tgcctggagc gtttctagca agtgagagag atgggagctc ctctcctgga 2100

ggattgcagg tggaactcag tcattagact cctcctccaa aggcccccta cgccaatcaa 2160

gggcaaaaag tctacatact ttcatcctga ctctgccccc tgctggctct tctgcccaat 2220

tggaggaaag caaccggtgg atcctcaaac aacactggtg tgacctgagg gcagaaaggt 2280

tctgcccggg aaggtcacca gcaccaacac cacggtagtg cctgaaattt caccattgct 2340

gtcaagttcc tttgggttaa gcattaccac tcaggcattt gactgaagat gcagctcact 2400

accctattct ctctttacgc ttagttatca gctttttaaa gtgggttatt ctggagtttt 2460

tgtttggaga gcacacctat cttagtggtt ccccaccgaa gtggactggc ccctgggtca 2520

gtctggtggg aggacggtgc aacccaagga ctgagggact ctgaagcctc tgggaaatga 2580

gaaggcagcc accagcgaat gctaggtctc ggactaagcc tacctgctct ccaagtctca 2640

gtggcttcat ctgtcaagtg ggatctgtca caccagccat acttatctct ctgtgctgtg 2700

gaagcaacag gaatcaagag ctgccctcct tgtccaccca cctatgtgcc aactgttgta 2760

actaggctca gagatgtgca cccatgggct ctgacagaaa gcagatacct caccctgcta 2820

cacatacagg atttgaactc agatctgtct gataggaatg tgaaagcacg gactcttact 2880

gctaactttt gtgtatcgta accagccaga tcctcttggt tatttgttta ccacttgtat 2940

tattaatgcc attatccctg aatccccctt gccaccccac cctccctgga gtgtggctga 3000

ggaggcctcc atctcatgta tcatctggat aggagcctgc tggtcacagc ctcctctgtc 3060

tgcccttcac cccagtggcc actcagcttc ctacccacac ctctgccaga agatcccctc 3120

aggactgcaa caggcttgtg caacaataaa tgttggcttg g 3161

<210> 31

<211> 643

<212> DNA

<213>mankind MTL5 gene

<400> 31

tggtgacgga gctcttaagc cccgagggtc cgttcgcttc ggagaacatc ggcctgaagg 60

cccccgtgaa gtacgaggag gacgagttcc acgtcttcaa agaagcgtac ctgggcccgg 120

cggaccccaa ggaacccgtc ctgcacgcgt tcaaccccgc gctgggcgcc gactgcaagg 180

gccaggtcaa ggcgaagctc gcggggggcg acagcgacgg cggggagctc ctcggggagt 240

accccgggat cccagagctc agcgcgctgg aggacgtcgc gctcctgcag gccccgcagc 300

cgcccgcctg caacgtgcac ttcctgtcct cgctgctacc cgcgcaccgc agcccggcgg 360

tgttgcccct gggcgcctgg gtcctggaag gagcctccca cccgggcgtc cgcatgatcc 420

cagttgaaat caaggaagca ggtggtacta ctacaagtaa taatccggaa gaagcaactt 480

tgcagaatct tcttgctcag gaatcctgtt gcaagttccc atcgtcccag gaactagagg 540

atgcctcctg ctgttctctt aagaaagatt ccaacccaat ggtaattatg ctaaagaaga 600

tgtgtaggac taacattata ctaaatgctg gaccccatca tta 643

<210> 32

<211> 1948

<212> DNA

<213>mankind FAM64A gene

<400> 32

cgcagagttt caaatcggct gcggtgggcg aggagcagct gtgctgcggc tgtgctcggc 60

cttagtggtg tcggggtcta gtggacagag aagactcttg gccaggcaga tggcttctcg 120

gtggcagaac atggggacct ccgtgcgccg gagatctctc cagcaccagg agcagctgga 180

ggacagcaag gagctgcagc ctgtggtcag ccatcaggag acctctgtag gggccctggg 240

gtccctgtgc agacagttcc aaaggaggct gcccctgaga gccgtcaacc tcaacctccg 300

cgcagggccc tcctggaaac gcctggaaac cccagagcca ggtcagcagg gcctccaggc 360

tgcagctcgc tcagctaaga gtgctttggg tgccgtgtcc cagagaatcc aggagtcctg 420

ccaaagtggc accaagtggc tggtggagac ccaggtgaag gccaggaggc ggaagagagg 480

agcacagaag ggcagtggat ccccaactca cagcctgagc cagaagagca cccggctgtc 540

tggagccgcc cctgcccact cagccgcaga cccctgggag aaggagcatc accgcctctc 600

tgtccggatg ggctcacatg cccacccatt acggcgatca aggcgggagg ctgccttccg 660

gagcccctac tcctcaacag agcccctctg ctctcccagc gagtctgaca gtgacctaga 720

gcctgtgggg gcgggaattc agcatctcca gaagctgtcc caagagctag atgaagccat 780

tatggcggaa gagagtggtg acatcgtctc tctcattcat gactgaggaa gtgcctgcag 840

gaaacaagcc ctgtctgacc gccaaggctt catactcaag gatgtctatg cttccccgtg 900

agcttcctgg aaaaaacccc cgggagtcgt cagtacccct gggccactgc taacaagcac 960

ctaacaaggg gcccagagcc ccctgctcca gccacatctg gacccatcag tgactgcctg 1020

ccatagcctg agagtgtctt ggggagacct tgcagagggg gagaattgtt ccttctgctt 1080

tcctagggga ctcttgagct tagaaactca tcgtacactt gaccttgagc cttctatttg 1140

cctcatctat aacatgaagt gctagcatca gatatttgag agctcttagc tctgtacccg 1200

ggtgcctggt ttttggggag tcatccgcag agtcactcac ccactgtgtt tctggtgcca 1260

aggctcttga gggccccact ctcatccctc ctttccctac cagggactcg gaggaaggca 1320

taggagatat ttccaggctt acgaccctgg gctcacgggt acctatttat atgctcagtg 1380

cagagcactg tggatgtgcc aggaggggta gccctgttca agagcaattt ctgccctttg 1440

taaattattt aagaaacctg ctttgtcatt ttattagaaa gaaaccagcg tgtgactttc 1500

ctagataaca ctgctttctc ataataaaga ctatttgcat ttgacatctg ttccctttca 1560

ccggcaacct ccaacctccc ccgccctccc acaccactct gggctataga tttgtaattt 1620

gtggtttggg gtctggccct catccacctc tgctccgagc cagtctgccc ccaatcccac 1680

cccagggaga acatgtagaa agggaaaggt ttctcaactt agttcagacc aaaacacatt 1740

gtaaaggcaa agaaggtttt tatttaagtg acaacatttg agagctaaaa accagctcac 1800

atcaaaatca agacccagtt gtaaaaatct tttaactcca taatgctgtt tttgtcttgt 1860

tagaaatctg atatcttaca ttagcgtttc taacggattt tgtacaaggc agccataagg 1920

aatataataa acctttttca ccacagaa 1948

<210> 33

<211> 1281

<212> DNA

<213>mankind SFTPD gene

<400> 33

tttgcttgga gctcctgggg cctaacaaaa agaaacctgc catgctgctc ttcctcctct 60

ctgcactggt cctgctcaca cagcccctgg gctacctgga agcagaaatg aagacctact 120

cccacagaac aatgcccagt gcttgcaccc tggtcatgtg tagctcagtg gagagtggcc 180

tgcctggtcg cgatggacgg gatgggagag agggccctcg gggcgagaag ggggacccag 240

gtttgccagg agctgcaggg caagcaggga tgcctggaca agctggccca gttgggccca 300

aaggggacaa tggctctgtt ggagaacctg gaccaaaggg agacactggg ccaagtggac 360

ctccaggacc tcccggtgtg cctggtccag ctggaagaga aggtcccctg gggaagcagg 420

ggaacatagg acctcagggc aagccaggcc caaaaggaga agctgggccc aaaggagaag 480

taggtgcccc aggcatgcag ggctcggcag gggcaagagg cctcgcaggc cctaagggag 540

agcgaggtgt ccctggtgag cgtggagtcc ctggaaacac aggggcagca gggtctgctg 600

gagccatggg tccccaggga agtccaggtg ccaggggacc cccgggattg aagggggaca 660

aaggcattcc tggagacaaa ggagcaaagg gagaaagtgg gcttccagat gttgcttctc 720

tgaggcagca ggttgaggcc ttacagggac aagtacagca cctccaggct gctttctctc 780

agtataagaa agttgagctc ttcccaaatg gccaaagtgt cggggagaag attttcaaga 840

cagcaggctt tgtaaaacca tttacggagg cacagctgct gtgcacacag gctggtggac 900

agttggcctc tccacgctct gccgctgaga atgccgcctt gcaacagctg gtcgtagcta 960

agaacgaggc tgctttcctg agcatgactg attccaagac agagggcaag ttcacctacc 1020

ccacaggaga gtccctggtc tattccaact gggccccagg ggagcccaac gatgatggcg 1080

ggtcagagga ctgtgtggag atcttcacca atggcaagtg gaatgacagg gcttgtggag 1140

aaaagcgtct tgtggtctgc gagttctgag ccaactgggg tgggtggggc agtgcttggc 1200

ccaggagttt ggccagaagt caaggcttag accctcatgc tgccaatatc ctaataaaaa 1260

ggtgaccatc tgtgccggga a 1281

<210> 34

<211> 3287

<212> DNA

<213>mankind BUB1 gene

<400> 34

attcgaatcg gcggcggctt ctagtttgcg gttcaggttt ggccgctgcc ggccagcgtc 60

ctctggccat ggacaccccg gaaaatgtcc ttcagatgct tgaagcccac atgcagagct 120

acaagggcaa tgaccctctt ggtgaatggg aaagatacat acagtgggta gaagagaatt 180

ttcctgagaa taaagaatac ttgataactt tactagaaca tttaatgaag gaatttttag 240

ataagaagaa ataccacaat gacccaagat tcatcagtta ttgtttaaaa tttgctgagt 300

acaacagtga cctccatcaa ttttttgagt ttctgtacaa ccatgggatt ggaaccctgt 360

catcccctct gtacattgcc tgggcggggc atctggaagc ccaaggagag ctgcagcatg 420

ccagtgctgt ccttcagaga ggaattcaaa accaggctga acccagagag ttcctgcaac 480

aacaatacag gttatttcag acacgcctca ctgaaaccca tttgccagct caagctagaa 540

cctcagaacc tctgcataat gttcaggttt taaatcaaat gataacatca aaatcaaatc 600

caggaaataa catggcctgc atttctaaga atcagggttc agagctttct ggagtgatat 660

cttcagcttg tgataaagag tcaaatatgg aacgaagagt gatcacgatt tctaaatcag 720

aatattctgt gcactcatct ttggcatcca aagttgatgt tgagcaggtt gttatgtatt 780

gcaaggagaa gcttattcgt ggggaatcag aattttcctt tgaagaattg agagcccaga 840

aatacaatca acggagaaag catgagcaat gggtaaatga agacagacat tatatgaaaa 900

ggaaagaagc aaatgctttt gaagaacagc tattaaaaca gaaaatggat gaacttcata 960

agaagttgca tcaggtggtg gagacatccc atgaggatct gcccgcttcc caggaaaggt 1020

ccgaggttaa tccagcacgt atggggccaa gtgtaggctc ccagcaggaa ctgagagcgc 1080

catgtcttcc agtaacctat cagcagacac cagtgaacat ggaaaagaac ccaagagagg 1140

cacctcctgt tgttcctcct ttggcaaatg ctatttctgc agctttggtg tccccagcca 1200

ccagccagag cattgctcct cctgttcctt tgaaagccca gacagtaaca gactccatgt 1260

ttgcagtggc cagcaaagat gctggatgtg tgaataagag tactcatgaa ttcaagccac 1320

agagtggagc agagatcaaa gaagggtgtg aaacacataa ggttgccaac acaagttctt 1380

ttcacacaac tccaaacaca tcactgggaa tggttcaggc aacgccatcc aaagtgcagc 1440

catcacccac cgtgcacaca aaagaagcat taggtttcat catgaatatg tttcaggctc 1500

ctacacttcc tgatatttct gatgacaaag atgaatggca atctctagat caaaatgaag 1560

atgcatttga agcccagttt caaaaaaatg taaggtcatc tggggcttgg ggagtcaata 1620

agatcatctc ttctttgtca tctgcttttc atgtgtttga agatggaaac aaagaaaatt 1680

atggattacc acagcctaaa aataaaccca caggagccag gacctttgga gaacgctctg 1740

tcagcagact tccttcaaaa ccaaaggagg aagtgcctca tgctgaagag tttttggatg 1800

actcaactgt atggggtatt cgctgcaaca aaaccctggc acccagtcct aagagcccag 1860

gagacttcac atctgctgca caacttgcgt ctacaccatt ccacaagctt ccagtggagt 1920

cagtgcacat tttagaagat aaagaaaatg tggtagcaaa acagtgtacc caggcgactt 1980

tggattcttg tgaggaaaac atggtggtgc cttcaaggga tggaaaattc agtccaattc 2040

aagagaaaag cccaaaacag gccttgtcgt ctcacatgta ttcagcatcc ttacttcgtc 2100

tgagccagcc tgctgcaggt ggggtactta cctgtgaggc agagttgggc gttgaggctt 2160

gcagactcac agacactgac gctgccattg cagaagatcc accagatgct attgctgggc 2220

tccaagcaga atggatgcag atgagttcac ttgggactgt tgatgctcca aacttcattg 2280

ttgggaaccc atgggatgat aagctgattt tcaaactttt atctgggctt tctaaaccag 2340

tgagttccta tccaaatact tttgaatggc aatgtaaact tccagccatc aagcccaaga 2400

ctgaatttca attgggttct aagctggtct atgtccatca ccttcttgga gaaggagcct 2460

ttgcccaggt gtacgaagct acccagggag atctgaatga tgctaaaaat aaacagaaat 2520

ttgttttaaa ggtccaaaag cctgccaacc cctgggaatt ctacattggg acccagttga 2580

tggaaagact aaagccatct atgcagcaca tgtttatgaa gttctattct gcccacttat 2640

tccagaatgg cagtgtatta gtaggagagc tctacagcta tggaacatta ttagatgatg 2700

aagatgattt atctgctggc ttggcactga ttgacctggg tcagagtata gatatgaaac 2760

tttttccaaa aggaactata ttcacagcaa agtgtgaaac atctggtttt cagtgtgttg 2820

agatgctcag caacaaacca tggaactacc agatcgatta ctttggggtt gctgcaacag 2880

tatattgcat gctctttggc acttacatga aagtgaaaaa tgaaggagga gagtgtaagc 2940

ctgaaggtct ttttagaagg cttcctcatt tggatatgtg gaatgaattt tttcatgtta 3000

tgttgaatat tccagattgt catcatcttc catctttgga tttgttaagg caaaagctga 3060

agaaagtatt tcaacaacac tatactaaca agattagggc cctacgtaat aggctaattg 3120

tactgctctt agaatgtaag cgttcacgaa aataaaattt ggatatagac agtccttaaa 3180

aatcacactg taaatatgaa tctgctcact ttaaacctgt ttttttttca tttattgttt 3240

atgtaaatgt ttgttaaaaa taaatcccat ggaatatttc catgtaa 3287

Claims (9)

1. a prognostic markers thing for pulmonary carcinoma, described label include the following assortment of genes: RP11-89K21.1, CDH17, GRIA1, CENPF, AP000438.2, HMMR, RP11-434D9.1, ADH1B, TPX2, OR7E47P, the sequence of described gene depends on Secondary for SEQ ID NO:1～10.

Prognostic markers thing the most according to claim 1, it is characterised in that described label also includes in following gene extremely A few gene: PLK1, HABP2, PBK, GPR37, NEK2, RP11-497G19.2, SLC2A1, PLOD2, ECT2, SPC25, institute The sequence stating gene is followed successively by SEQ ID NO:11～20.

Prognostic markers thing the most according to claim 2, it is characterised in that described label also includes in following gene extremely Few gene a: RP5-839B4.8, SKA3, C20orf197, LYPD3, E2F7, CPS1, ABCA3, TICRR, CDKN3, VIPR1, MTL5, FAM64A, SFTPD, BUB1, the sequence of described gene is followed successively by SEQ ID NO:21～34.

Prognostic markers thing the most according to claim 1, it is characterised in that described pulmonary carcinoma is adenocarcinoma of lung.

5. a method for prediction of prognosis for pulmonary carcinoma, comprises the following steps:

1) gather sample from patients with lung cancer, detect and obtain the expression of the prognostic markers thing of pulmonary carcinoma；

2) expression obtained is applied to the prognostic predictive model of pulmonary carcinoma, to obtain lung cancer for prognosis value-at-risk；

3) obtained lung cancer for prognosis value-at-risk is compared with reference value, determine the prognosis of described patient；

The prognostic markers thing of described pulmonary carcinoma is described in any one of Claims 1 to 4；

The prognostic predictive model of described pulmonary carcinoma is:

S=∑Pi * Ci

Wherein, S is lung cancer for prognosis value-at-risk, and Pi is the quantitative expression value of i-th bit gene, and Ci represents the COX of i-th bit gene and returns Return coefficient, i=1,2,3,4 ... n, n are the sum of the prognostic markers thing of pulmonary carcinoma.

Method the most according to claim 5, it is characterised in that the Index for diagnosis method of patient:

Compare with reference value according to obtained lung cancer for prognosis value-at-risk, if less than or equal to reference value, detected patient just belongs to Low-risk, if greater than reference value, detection patient just belongs to low-risk.

Method the most according to claim 5, it is characterised in that COX regression coefficient in the prognostic predictive model of described pulmonary carcinoma Determine by the following method:

1) the multidigit patients with lung cancer collected is about the transcript profile data of the prognostic markers thing of pulmonary carcinoma and clinical Survival data；

2) use the prognostic markers thing of the multifactor above-mentioned pulmonary carcinoma of COX model stepwise regression analysis, obtain COX regression coefficient；

Described reference value determines by the following method: turning according to the multidigit patients with lung cancer of gained COX regression coefficient and above-mentioned collection Record group data, calculate multidigit patients with lung cancer lung cancer for prognosis value-at-risk by the prognostic predictive model of pulmonary carcinoma, take multidigit patients with lung cancer lung The median of cancer prognostic risk value is as reference value.

8., for assessing the test kit of lung cancer for prognosis life cycle, it includes requiring described in 1～4 any one for test right The mRNA of label or the reagent of protein expression level.

Test kit the most according to claim 8, it is characterised in that for detecting the reagent of the mrna expression level of label It is the primer and/or probe being combined with described gene specific；It is right for detecting the reagent of the protein expression level of label The antibody that the albumen of described gene code is special.