CN114479143A - Preparation method and application of edible anthocyanin preservative film for shrimps with preservation effect - Google Patents

Preparation method and application of edible anthocyanin preservative film for shrimps with preservation effect Download PDF

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CN114479143A
CN114479143A CN202210205980.3A CN202210205980A CN114479143A CN 114479143 A CN114479143 A CN 114479143A CN 202210205980 A CN202210205980 A CN 202210205980A CN 114479143 A CN114479143 A CN 114479143A
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film
edible
anthocyanin
solution
chitosan
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孔保华
王强
李香澳
徐舰航
陈倩
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Northeast Agricultural University
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    • CCHEMISTRY; METALLURGY
    • C08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08JWORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J5/00Manufacture of articles or shaped materials containing macromolecular substances
    • C08J5/18Manufacture of films or sheets
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23BPRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
    • A23B4/00General methods for preserving meat, sausages, fish or fish products
    • A23B4/14Preserving with chemicals not covered by groups A23B4/02 or A23B4/12
    • A23B4/18Preserving with chemicals not covered by groups A23B4/02 or A23B4/12 in the form of liquids or solids
    • A23B4/20Organic compounds; Microorganisms; Enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • CCHEMISTRY; METALLURGY
    • C08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08JWORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2305/00Characterised by the use of polysaccharides or of their derivatives not provided for in groups C08J2301/00 or C08J2303/00
    • C08J2305/08Chitin; Chondroitin sulfate; Hyaluronic acid; Derivatives thereof
    • CCHEMISTRY; METALLURGY
    • C08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08KUse of inorganic or non-macromolecular organic substances as compounding ingredients
    • C08K5/00Use of organic ingredients
    • C08K5/04Oxygen-containing compounds
    • C08K5/15Heterocyclic compounds having oxygen in the ring
    • C08K5/151Heterocyclic compounds having oxygen in the ring having one oxygen atom in the ring
    • C08K5/1545Six-membered rings

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  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Manufacturing & Machinery (AREA)
  • Chemical Kinetics & Catalysis (AREA)
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  • Medicinal Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Materials Engineering (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Food Science & Technology (AREA)
  • General Preparation And Processing Of Foods (AREA)

Abstract

The invention provides an edible anthocyanin preservative film for prawns with a preservative effect according to the performance characteristics of chitosan and blueberry anthocyanin, has the characteristics of greenness, environmental protection, no toxicity, harmlessness and edibility, and also provides a preparation method of the edible anthocyanin preservative film. The edible film can be applied to inner packaging of seafood products such as fish, shrimp and the like. The chitosan solution is used as the raw material in the experiment, and is the only alkaline polysaccharide in nature, so that the chitosan solution has the performances of no toxicity, degradability, capability of being adsorbed on mucosa, high biological activity and the like. The edible film prepared by the invention has good grease oxidation resistance and antibacterial property, and is a safe, nontoxic and biodegradable natural preservative film. The preparation method of the edible film disclosed by the invention is easy to operate, short in production period and high in application and popularization values.

Description

Preparation method and application of edible anthocyanin preservative film for shrimps with preservation effect
Technical Field
The invention relates to a preparation method and application of an edible anthocyanin preservative film for prawns with a preservation effect, and belongs to the field of food materials.
Background
The preservative film, which is a material technology, is popular in the 40 th 19 th century, and along with the improvement of living standard, the improvement of the quality requirement of people on life prompts the application of the preservative film to be wider. The preservative film has proper oxygen permeability and moisture permeability, adjusts the content of oxygen and moisture around the preserved product, and blocks dust, thereby achieving the effect of prolonging the preservation period of food. Under the era of gradually improved economic development, the requirements on the quality of food are improved, aquatic products become obvious on dining tables of people, and shrimps contain more high-quality protein and essential amino acid required by human bodies and are a source of excellent protein, so the shrimps are popular with the people. However, due to the unique physiological and biochemical characteristics of the shrimps, the shrimps are very easy to be polluted in the processing process, and the shrimps are corroded by microorganisms and cannot have a long-time fresh-keeping effect, so that the shrimps become common dishes on a dining table and are limited. Chitosan is a basic polysaccharide which is rich in resources, safe and nontoxic, and with the continuous development of modern science and technology, a nano material also becomes a mainstream research trend, and chitosan nanoparticles are applied to an edible composite film as a nano filler due to higher antibacterial property so as to increase and improve the mechanical property and barrier property of the edible composite film. The blueberry anthocyanin edible film has the advantages that the oxidation resistance, the water resistance and the bacterial inhibition delay the corrosion of shrimps, the blueberry anthocyanin is added into the chitosan film solution with good film forming property, degradability and high mechanical strength, and the film barrier for delaying the respiration and isolating the contact with microorganisms is formed, so that the fresh-keeping time of the shrimps can be effectively prolonged, a larger market prospect is created for the development of the shrimp food industry, and the requirements of people on the shrimps are met.
Disclosure of Invention
According to the performance characteristics of chitosan and blueberry anthocyanin, the invention provides the edible preservative film with the function of indicating the preservation of shrimps, and the edible preservative film has the characteristics of environmental protection, no toxicity, no harm and edible performance; also provides a preparation method thereof.
In order to achieve the purpose, the invention adopts the following technical scheme: firstly, respectively preparing a proanthocyanidin aqueous solution with the mass concentration of 0.5-1.5% and a chitosan aqueous solution with the mass concentration of 1-2%, then mixing the proanthocyanidin and the chitosan solution according to the dry weight ratio of 1:50-3:50 under the stirring state, fully and uniformly stirring to form a film forming solution, and then preparing the film by film forming. The film-making method comprises the steps of pouring the film-forming solution into a film-making mold, naturally drying in an oven at 25 ℃, and then uncovering the film.
An edible preservative film with a shrimp preservation function comprises the following raw materials: chitosan nanoparticles, anthocyanin and glacial acetic acid. The manufacturing process comprises the following steps:
(1) firstly, 20g of acetic acid is dissolved in 1000ml of beaker to prepare 2 percent acetic acid-containing deionized water;
(2) taking 60ml of deionized water obtained in the step (1), and dissolving 0.6g of chitosan in the 2% acetic acid-containing deionized water to prepare 1% chitosan coating solution;
(3) dissolving in a magnetic stirrer at 50 deg.C for 20min to obtain gel;
(4) adding 0.3mg/ml anthocyanin into the gel solution;
(5) after the (4) and the gel solution are completely dissolved, homogenizing for 5min at the rotating speed of 10000r/min by using a high-speed homogenizer to obtain a membrane liquid of the composite edible membrane;
(6) uniformly pouring the film liquid on a disposable polyethylene disc by a casting coating method, drying in an oven at the temperature of 25 ℃ for 12h to form a film, and then removing the film for use.
The edible film can be applied to inner packaging of seafood products such as fish, shrimp and the like.
Compared with the existing edible film, the material prepared by the invention has the following advantages:
the raw material adopted in the experiment is chitosan solution which is the only alkaline polysaccharide in nature, so that the chitosan solution has the performances of no toxicity, degradability, capability of being adsorbed on mucosa, high biological activity and the like.
The edible film prepared by the invention has good grease oxidation resistance and antibacterial property, and is a safe, nontoxic and biodegradable natural preservative film.
The preparation method of the edible film disclosed by the invention is easy to operate, short in production period and high in application and popularization values.
Detailed Description
The technical solutions of the present invention are further described below with reference to the following embodiments of the present invention, which are used for explaining the present invention and do not limit the present invention.
Example 1
Respectively preparing a procyanidine aqueous solution with the mass concentration of 0.5% and a chitosan aqueous solution with the mass concentration of 0.5%, then mixing the procyanidine and the collagen solution according to the dry weight ratio of 1:50 in a stirring state, fully and uniformly stirring to form a film forming solution, then pouring the film forming solution into a tetrafluoroethylene mold with the diameter, and putting the tetrafluoroethylene mold into an oven with the temperature of 25 ℃ for drying to form the film.
The preparation process comprises the following steps:
(1) firstly, 20g of acetic acid is dissolved in 1000ml of beaker to prepare 2 percent acetic acid-containing deionized water;
(2) taking 60ml of deionized water obtained in the step (1), and dissolving 0.3g of chitosan in the 2% acetic acid-containing deionized water to prepare 1% chitosan coating solution;
(3) dissolving in a magnetic stirrer at 50 deg.C for 20min to obtain gel;
(4) adding 0.1mg/ml anthocyanin into the gel solution;
(5) after the (4) and the gel solution are completely dissolved, homogenizing for 5min at the rotating speed of 10000r/min by using a high-speed homogenizer to obtain a membrane liquid of the composite edible membrane;
(6) uniformly pouring the film liquid on a disposable polyethylene disc by a casting coating method, drying in an oven at the temperature of 25 ℃ for 12h, and removing the film to use after film forming.
Example 2
Respectively preparing a procyanidine aqueous solution with the mass concentration of 1% and a chitosan aqueous solution with the mass concentration of 1%, then mixing the procyanidine and the collagen solution according to the dry weight ratio of 2:50 under the stirring state, fully and uniformly stirring to form a film forming solution, then pouring the film forming solution into a tetrafluoroethylene mold with the diameter, and drying in an oven at 25 ℃ to form the film.
The preparation process comprises the following steps:
(1) firstly, 20g of acetic acid is dissolved in 1000ml of beaker to prepare 2 percent acetic acid-containing deionized water;
(2) taking 60ml of deionized water obtained in the step (1), and dissolving 0.6g of chitosan in the 2% acetic acid-containing deionized water to prepare 1% chitosan coating solution;
(3) dissolving in a magnetic stirrer at 50 deg.C for 20min to obtain gel;
(4) adding 0.2mg/ml anthocyanin into the gel solution;
(5) after the (4) and the gel solution are completely dissolved, homogenizing for 5min at the rotating speed of 10000r/min by using a high-speed homogenizer to obtain a membrane liquid of the composite edible membrane;
(6) uniformly pouring the film liquid on a disposable polyethylene disc by a casting coating method, drying in an oven at the temperature of 25 ℃ for 12h, and removing the film to use after film forming.
Example 3
Respectively preparing a procyanidine aqueous solution with the mass concentration of 1.5% and a chitosan aqueous solution with the mass concentration of 1.5%, then mixing the procyanidine and the collagen solution according to the dry weight ratio of 3:50 in a stirring state, fully and uniformly stirring to form a film forming solution, then pouring the film forming solution into a tetrafluoroethylene mold with the diameter, and putting the tetrafluoroethylene mold into an oven with the temperature of 25 ℃ for drying to form the film.
The preparation process comprises the following steps:
(1) firstly, 20g of acetic acid is dissolved in 1000ml of beaker to prepare 2 percent acetic acid-containing deionized water;
(2) taking 60ml of deionized water obtained in the step (1), and dissolving 0.9g of chitosan in the 2% acetic acid-containing deionized water to prepare 1% chitosan coating solution;
(3) dissolving in a magnetic stirrer at 50 deg.C for 20min to obtain gel;
(4) adding 0.3mg/ml anthocyanin into the gel solution;
(5) after the (4) and the gel solution are completely dissolved, homogenizing for 5min at the rotating speed of 10000r/min by using a high-speed homogenizer to obtain a membrane liquid of the composite edible membrane;
(6) uniformly pouring the film liquid on a disposable polyethylene disc by a casting coating method, drying in an oven at the temperature of 25 ℃ for 12h, and removing the film to use after film forming.
Example 4
Respectively preparing a procyanidine aqueous solution with the mass concentration of 2% and a chitosan aqueous solution with the mass concentration of 2%, then mixing the procyanidine and the collagen solution according to the dry weight ratio of 4:50 under the stirring state, fully and uniformly stirring to form a film forming solution, then pouring the film forming solution into a tetrafluoroethylene mold with the diameter, and drying in an oven at 25 ℃ to form the film. The preparation process comprises the following steps:
(1) firstly, 20g of acetic acid is dissolved in 1000ml of beaker to prepare 2 percent acetic acid-containing deionized water;
(2) taking 60ml of deionized water obtained in the step (1), and dissolving 1.2g of chitosan in the 2% acetic acid-containing deionized water to prepare a 2% chitosan coating solution;
(3) dissolving in a magnetic stirrer at 50 deg.C for 20min to obtain gel;
(4) adding 0.4mg/ml anthocyanin into the gel solution;
(5) after the (4) and the gel solution are completely dissolved, homogenizing for 5min at the rotating speed of 10000r/min by using a high-speed homogenizer to obtain a membrane liquid of the composite edible membrane;
(6) uniformly pouring the film liquid on a disposable polyethylene disc by a casting coating method, drying in an oven at the temperature of 25 ℃ for 12h, and removing the film to use after film forming.
Table 1 below shows the results of the fresh keeping performance analysis of shrimps using the anthocyanin edible films of examples 1 to 4 of the present invention and comparative example.
TABLE 1
Figure BDA0003523091910000031
The result of the prawn preservation performance analysis of the anthocyanin edible films of the examples 1 to 4 and the comparative example can obtain that the prawn preservation performance of the anthocyanin edible films of the examples 1 to 4 is obviously better than that of the comparative example. The texture of shrimp tissue is affected by myofibrillar and connective tissue protein, and after the shrimp die, microorganisms breed on the body surface, and the shrimp meat texture is changed by enzyme with the function of hydrolyzing protein and the putrefaction function of microorganisms.
Finally, it should be noted that: the above embodiments are only used to help understanding the technical solutions and core ideas of the present invention, and are not limited thereto; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: it is possible to modify the solutions described in the embodiments or to substitute some or all of the technical features of the embodiments, and these modifications or substitutions also fall within the scope of the claims of the present invention.

Claims (3)

1. A preparation method and application of an edible anthocyanin preservative film for prawns with a preservation effect comprise the following steps: firstly, respectively preparing a proanthocyanidin aqueous solution with the mass concentration of 0.5-1.5% and a chitosan aqueous solution with the mass concentration of 1-2%, then mixing the proanthocyanidin and the chitosan solution according to the dry weight ratio of 1:50-3:50 under the stirring state, fully and uniformly stirring to form a film forming solution, and then preparing the film by film forming. The film-making method comprises the steps of pouring the film-forming solution into a film-making mold, naturally drying in an oven at 25 ℃, and then uncovering the film.
The manufacturing process comprises the following steps:
(1) firstly, 20g of acetic acid is dissolved in 1000ml of beaker to prepare 2 percent acetic acid-containing deionized water;
(2) taking 60ml of deionized water obtained in the step (1), and dissolving 0.6g of chitosan in the 2% acetic acid-containing deionized water to prepare 1% chitosan coating solution;
(3) dissolving in a magnetic stirrer at 50 deg.C for 20min to obtain gel;
(4) adding 0.3mg/ml anthocyanin into the gel solution;
(5) after the (4) and the gel solution are completely dissolved, homogenizing for 5min at the rotating speed of 10000r/min by using a high-speed homogenizer to obtain a membrane liquid of the composite edible membrane;
(6) uniformly pouring the film liquid on a disposable polyethylene disc by a casting coating method, drying in an oven at the temperature of 25 ℃ for 12h to form a film, and then removing the film for use.
2. The anthocyanin edible film prepared in the embodiments 1 to 4 of the invention is systematically evaluated for the effect of the prawn preservation performance through texture measurement, and the prawn which does not utilize the anthocyanin edible film in the comparative example is used as a reference.
3. The result of the prawn preservation performance analysis of the anthocyanin edible films of the examples 1 to 4 and the comparative example can obtain that the prawn preservation performance of the anthocyanin edible films of the examples 1 to 4 is obviously better than that of the comparative example. The texture of shrimp tissue is affected by myofibrillar and connective tissue protein, and after the shrimp die, microorganisms breed on the body surface, and the shrimp meat texture is changed by enzyme with the function of hydrolyzing protein and the putrefaction function of microorganisms.
CN202210205980.3A 2022-02-28 2022-02-28 Preparation method and application of edible anthocyanin preservative film for shrimps with preservation effect Pending CN114479143A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116023716A (en) * 2022-10-31 2023-04-28 华中农业大学 Layer-by-layer nanoparticle filled fresh-keeping-indicating composite film, preparation method and application

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030091851A1 (en) * 2001-10-31 2003-05-15 National University Of Singapore Method of preparing chitin films
CN105111474A (en) * 2015-09-11 2015-12-02 江苏大学 Method for preparing chitosan and gelatin composite membrane based on anthocyanidin
CN111234325A (en) * 2020-03-13 2020-06-05 武汉轻工大学 Preparation method of food packaging film and milk packaging structure
CN113861470A (en) * 2021-10-26 2021-12-31 东北农业大学 Preparation method of edible gellan gum-chitosan-anthocyanin film
CN113968986A (en) * 2021-11-09 2022-01-25 浙江工商大学 Preparation method of purple cabbage anthocyanin-collagen chitosan composite intelligent membrane

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030091851A1 (en) * 2001-10-31 2003-05-15 National University Of Singapore Method of preparing chitin films
CN105111474A (en) * 2015-09-11 2015-12-02 江苏大学 Method for preparing chitosan and gelatin composite membrane based on anthocyanidin
CN111234325A (en) * 2020-03-13 2020-06-05 武汉轻工大学 Preparation method of food packaging film and milk packaging structure
CN113861470A (en) * 2021-10-26 2021-12-31 东北农业大学 Preparation method of edible gellan gum-chitosan-anthocyanin film
CN113968986A (en) * 2021-11-09 2022-01-25 浙江工商大学 Preparation method of purple cabbage anthocyanin-collagen chitosan composite intelligent membrane

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116023716A (en) * 2022-10-31 2023-04-28 华中农业大学 Layer-by-layer nanoparticle filled fresh-keeping-indicating composite film, preparation method and application
CN116023716B (en) * 2022-10-31 2024-03-29 华中农业大学 Layer-by-layer nanoparticle filled fresh-keeping-indicating composite film, preparation method and application

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Application publication date: 20220513