CN104719429B - The method that jelly-fish fresh-keeping and processing are carried out using hurdle technology - Google Patents
The method that jelly-fish fresh-keeping and processing are carried out using hurdle technology Download PDFInfo
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Abstract
Description
技术领域technical field
本发明涉及海蜇的保鲜及加工方法,更具体地说,涉及刚捕捞的新鲜海蜇以及盐渍海蜇的保鲜及加工方法。The invention relates to a fresh-keeping and processing method of jellyfish, more specifically, to a fresh-keeping and processing method of just caught fresh jellyfish and salted jellyfish.
背景技术Background technique
海蜇是海洋中分布广泛的腔肠动物之一,也是我国海水养殖业常见的经济型水产品,2013年全国海蜇海水养殖产量已达到66513t。海蜇具有很高的食用价值和药用价值,其高蛋白低脂肪的特点及独特的口感得到了很多女性消费者的青睐。此外,海蜇还具有一定的药用价值,本草纲目中记载海蜇性咸、平,入肝肾,可消热化痰、消肿解毒、软坚散结,治热痰、气管炎、哮喘、口噪咽干、阴虚便秘、淋巴结核、高血压等症。对于海蜇的研究表明海蜇体内含有多种生物活性物质,如:在海蜇胶原蛋白的提取及活性研究方面,郝更新等人发表了“海蜇皮营养成分分析及胶原蛋白的提取”(《农产品加工:学刊(下)2011年4期),陶字等人发表了“不同方法提取沙海蜇皮胶原蛋白的对比分析”(《食品工业科技》2012年14期),刘磊等人发表的“海蜇胶原蛋白的提取及纯化”(《天然产物研究与开发》2011年1期),丁进锋等人对从海蜇中提取的胶原蛋白活性进行了研究,证实了海蜇胶原具有明显的促愈合作用(《食品科学》2010年24期)。在对海蜇胶原肽的研究方面,丁进锋等的研究比较多,发表了“海蜇胶原蛋白肽的免疫活性的研究”(《水产科学》2011年6期),“海蜇胶原蛋白肽的降血脂及抗氧化作用的研究”(《天然产物研究与开发》2012年24期)。任国艳等人对于海蜇糖蛋白的研究较多,先后发表了“海蜇头糖蛋白分离纯化及免疫活性研究”、“海蜇糖蛋白及其糖肽的免疫活性”、“海蜇糖肽对小鼠脾脏细胞因子mRNA表达的影响”(《食品科学》2011年17期;2013年17期;2013年23期)。在专利方面,中国科学院海洋研究所2007年申报了“一种从海蜇触手中提取毒素的方法”(申请号200710016372.3),山东好当家海洋发展股份有限公司2008年申报了“一种海蜇活性肽酸奶及其制备方法”(申请号200810158085.0),李亚有2011年申报了“一种海蜇降压肽的制备方法”(申请号201110256019.9)等。Jellyfish is one of the coelenterates widely distributed in the ocean, and it is also a common economical aquatic product in my country's mariculture industry. In 2013, the national jellyfish mariculture production reached 66,513 tons. Jellyfish has high edible value and medicinal value, and its high protein and low fat characteristics and unique taste have won the favor of many female consumers. In addition, jellyfish also has a certain medicinal value. It is recorded in the Compendium of Materia Medica that jellyfish is salty and flat, and enters the liver and kidneys. Dry throat, Yin deficiency, constipation, lymphatic tuberculosis, high blood pressure and other diseases. The research on jellyfish shows that jellyfish contains multiple biologically active substances, such as: in terms of the extraction and activity research of jellyfish collagen, Hao Gengxin and others published "Jellyfish Skin Nutrient Component Analysis and Collagen Extraction" ("Agricultural Product Processing: Academic Journal (Part 2, 2011, Issue 4), Tao Zi et al. published "Comparative Analysis of Extracting Collagen from Sand Jellyfish Skin by Different Methods" ("Food Industry Science and Technology" 2012 Issue 14), Liu Lei et al. published "Jellyfish Collagen Extraction and purification of protein" ("Natural Product Research and Development" 2011 Issue 1), Ding Jinfeng and others studied the activity of collagen extracted from jellyfish, and confirmed that jellyfish collagen has obvious healing effect ("Food Science "2010 Issue 24). In terms of research on jellyfish collagen peptides, Ding Jinfeng et al. have done a lot of research, and published "Research on the Immunological Activity of Jellyfish Collagen Peptides" ("Aquatic Science" 2011 Issue 6), "Jellyfish Collagen Peptides in Lowering Blood Lipid and Anti- Research on Oxidation" ("Natural Product Research and Development" 2012 Issue 24). Ren Guoyan and others have done a lot of research on jellyfish glycoprotein, and successively published "Research on the Isolation, Purification and Immunological Activity of Jellyfish Head Glycoprotein", "Immunological Activity of Jellyfish Glycoprotein and Its Glycopeptide", "Jellyfish Glycopeptide Effect on Mice Spleen The influence of cytokine mRNA expression" ("Food Science" 2011, 17; 2013, 17; 2013, 23). In terms of patents, the Institute of Oceanology, Chinese Academy of Sciences declared "a method for extracting toxins from jellyfish tentacles" in 2007 (application number 200710016372.3), and Shandong Haodangjia Ocean Development Co., Ltd. declared "a jellyfish active peptide yoghurt" in 2008. and its preparation method” (application number 200810158085.0), Li Yayou declared “a preparation method of jellyfish antihypertensive peptide” (application number 201110256019.9) in 2011, etc.
海蜇的成熟季节一般在7-9月,新鲜海蜇味道鲜美,营养丰富,沿海的渔民对于新鲜海蜇的烹调方式有很多,如:炒、炖、炸、凉拌等,然而这些美味对于其它地区的消费者来说很难实现,这是由于新鲜海蜇体内含有大量的水分,打捞上岸后若不及时进行加工处理,会迅速自溶,水分大量流失,造成品质劣化。目前对于新鲜海蜇的处理方法主要是盐矾加工,盐矾的加入能够使海蜇组织迅速脱水,蛋白凝固变性,达到长期保存的目的,但食用盐矾海蜇会造成铝的摄入,因此,代替盐矾处理,开发新的海蜇保鲜方法也是目前研究的热点,如刘日雪2013年申报了“一种海蜇的腌制方法(申请号201310206542.X)”。除此之外,对于新鲜海蜇的保鲜方法还鲜有报道。The ripening season of jellyfish is generally from July to September. Fresh jellyfish is delicious and nutritious. Coastal fishermen cook fresh jellyfish in many ways, such as: frying, stewing, frying, cold dressing, etc. However, these delicacies are not suitable for consumption in other regions. This is because fresh jellyfish contains a lot of water. If it is not processed in time after being salvaged, it will autolyze quickly, and a large amount of water will be lost, resulting in quality deterioration. At present, the processing method for fresh jellyfish is mainly salt alum processing. The addition of salt alum can quickly dehydrate the jellyfish tissue, coagulate and denature the protein, and achieve the purpose of long-term preservation. However, eating salt alum jellyfish will cause aluminum intake. Therefore, instead of salt Alum treatment, the development of new jellyfish fresh-keeping method is also the focus of current research, such as Liu Rixue declared "a kind of jellyfish pickling method (application number 201310206542.X)" in 2013. In addition, there are few reports for the preservation method of fresh jellyfish.
随着人们对食品保鲜研究的深入,对于保鲜理论也有了新的认识,目前的一些研究普遍认为采用多种手段结合的方式可有效延长食品保质期。栅栏因子理论认为,食品要达到可贮性与卫生安全性,其内部必须存在能够阻止食品所含腐败菌和病原菌生长繁殖的因子,这些因子通过临时和永久性地打破微生物的内平衡(微生物处于正常状态下内部环境的稳定和统一),保持食品品质。这些因子被称为栅栏因子。这些因子及其互作效应决定了食品的微生物稳定性,这就是栅栏效应。目前采用栅栏技术对食品进行保鲜的实例也比较多,例如“一种利用栅栏技术的鸡肉保鲜方法”(申请号201110076318.4)、“一种有机油焖笋的防腐保鲜加工工艺”(申请号:200610164448.2)。With the in-depth research on food preservation, people have new understanding of the theory of preservation. Some current studies generally believe that the combination of various methods can effectively prolong the shelf life of food. The fence factor theory holds that in order to achieve storability and hygienic safety in food, there must be factors that can prevent the growth and reproduction of spoilage bacteria and pathogenic bacteria contained in food. These factors break the internal balance of microorganisms temporarily and permanently (the microorganisms are in Stability and unity of the internal environment under normal conditions) to maintain food quality. These factors are called fence factors. These factors and their interaction effects determine the microbial stability of food, which is the fence effect. There are also many examples of using fence technology to keep food fresh at present, such as "a chicken fresh-keeping method utilizing fence technology" (application number 201110076318.4), "an antiseptic and fresh-keeping processing technology for braised bamboo shoots in organic oil" (application number: 200610164448.2 ).
冷冻是一种比较传统的保鲜方法,也是水产品常用的保鲜方法。低温环境下微生物和酶的活性被抑制,从而实现水产品的保鲜。常见的水产品如鱼类、贝类、虾蟹等都可以采用冷冻进行保鲜,但很少有对新鲜海蜇进行冷冻保鲜的报道,这是因为新鲜海蜇的含水量极高,冻融过程中海蜇的水分会以冰晶的形式流失,造成海蜇口感的改变及水溶性营养成分的流失。Freezing is a relatively traditional fresh-keeping method, and it is also a commonly used fresh-keeping method for aquatic products. The activity of microorganisms and enzymes is inhibited in a low-temperature environment, thereby realizing the preservation of aquatic products. Common aquatic products such as fish, shellfish, shrimp and crab can be kept fresh by freezing, but there are few reports on fresh jellyfish by freezing and keeping fresh. This is because the water content of fresh jellyfish is extremely high. The water in the jellyfish will be lost in the form of ice crystals, resulting in a change in the taste of the jellyfish and a loss of water-soluble nutrients.
发明内容Contents of the invention
本发明的目的在于采用栅栏技术对新鲜海蜇及盐渍海蜇进行保鲜及加工,对于新鲜海蜇,将漂洗、热烫、酸处理、低温保藏作为栅栏因子,同时,为减少保藏过程中海蜇水分的流失,在此基础上利用浓度梯度的食用酒精溶液对海蜇进行浸泡,降低海蜇组织的冻结点,减少冷冻对海蜇组织造成的损伤,实现新鲜海蜇的长期保存。The purpose of the present invention is to adopt barrier technology to preserve and process fresh jellyfish and salted jellyfish. For fresh jellyfish, rinsing, blanching, acid treatment, and low-temperature preservation are used as barrier factors. On this basis, the jellyfish is soaked in the edible alcohol solution with a concentration gradient, the freezing point of the jellyfish tissue is lowered, the damage caused by freezing to the jellyfish tissue is reduced, and the long-term preservation of the fresh jellyfish is realized.
对于新鲜海蜇原料,为了达到上述目的,本发明提供了一种利用栅栏技术进行海蜇保鲜及加工的方法,包括如下步骤:For fresh jellyfish raw materials, in order to achieve the above object, the present invention provides a method for utilizing fence technology to preserve and process jellyfish, comprising the following steps:
S1:前处理:新鲜海蜇,按伞、胃柱、肩板、口腕、生殖腺进行分割;S1: Pretreatment: fresh jellyfish, divided according to umbrella, stomach column, shoulder plate, mouth wrist and gonad;
S2:漂洗:纯净水冲洗2~5次;S2: Rinse: rinse with pure water for 2 to 5 times;
S3:热烫减菌:在60~80℃的热水中热烫,热烫时间为:30~60s;S3: blanching to reduce bacteria: blanching in hot water at 60-80°C, blanching time: 30-60s;
S4:以下方式择一进行酸处理抑菌:S4: Choose one of the following methods for acid treatment to inhibit bacteria:
方式1:醋酸溶液浸泡,将海蜇置于醋酸含量为0.03~0.15g/100mL的溶液中浸泡,料液比为1:5~1:20,浸泡时间为3~5min;Method 1: Soak in acetic acid solution, soak the jellyfish in a solution with an acetic acid content of 0.03-0.15g/100mL, the ratio of solid to liquid is 1:5-1:20, and the soaking time is 3-5 minutes;
方式2:柠檬酸溶液浸泡,将海蜇置于柠檬酸含量为0.05~0.2g/100mL的溶液中进行浸泡,料液比为1:5~1:20,浸泡时间为3~5min;Method 2: Soaking in citric acid solution, soaking the jellyfish in a solution with a citric acid content of 0.05-0.2g/100mL, the solid-liquid ratio is 1:5-1:20, and the soaking time is 3-5 minutes;
S5:浓度梯度抗冻处理S5: concentration gradient antifreeze treatment
将海蜇在体积比5%、10%、15%、20%的梯度食用酒精溶液中分别浸泡30min,料液比为1:2~1:10;Soak the jellyfish for 30 minutes in gradient edible alcohol solutions with a volume ratio of 5%, 10%, 15%, and 20%, respectively, with a solid-liquid ratio of 1:2 to 1:10;
S6:贮藏S6: Storage
将抗冻处理后的海蜇与体积比为20%的食用酒精水溶液按照质量比为1:1混合包装后放入-10~-18℃低温环境中进行保藏。The antifreeze-treated jellyfish and 20% edible alcohol aqueous solution by volume are mixed and packaged according to a mass ratio of 1:1, and then stored in a low-temperature environment of -10 to -18°C.
对于盐渍海蜇原料,为了达到上述目的,本发明提供了一种利用栅栏技术进行海蜇保鲜及加工的方法,包括如下步骤:For the raw material of salted jellyfish, in order to achieve the above object, the present invention provides a method for fresh-keeping and processing jellyfish utilizing fence technology, comprising the following steps:
S1:海蜇加工废弃物的酶解S1: Enzymatic hydrolysis of jellyfish processing waste
采用胰蛋白酶对盐渍海蜇加工废弃物冻干粉进行酶解,酶解工艺为:按照海蜇冻干粉质量30~50倍比例加入去离子水,振荡混合,然后用6mol/L HCl和6mol/L NaOH调节混合浆液pH至pH8.0,在37℃下保温10min后,根据海蜇冻干粉质量以每克冻干粉加入3000U酶活力单位加入胰蛋白酶进行酶解反应,反应过程中用酸和碱调节始终保持pH8.0,酶解时间为3~5h;酶解结束后,混合浆液在95~100℃沸水浴中加热10min;再调节浆液pH至中性;最后将混合浆液在4℃下7000r/min离心20min,取上清液为海蜇酶解物,真空冷冻干燥后,-20~-80℃保存备用;Use trypsin to enzymatically hydrolyze the freeze-dried powder of salted jellyfish processing waste. The enzymatic hydrolysis process is: add deionized water according to the ratio of 30 to 50 times the mass of jellyfish freeze-dried powder, shake and mix, and then use 6mol/L HCl and 6mol/L Adjust the pH of the mixed slurry to pH8.0 with L NaOH, and after incubating at 37°C for 10 minutes, add 3000 U of enzyme activity units per gram of freeze-dried jellyfish powder according to the quality of jellyfish freeze-dried powder and add trypsin for enzymatic hydrolysis reaction. Alkali adjustment always keeps the pH at 8.0, and the enzymolysis time is 3 to 5 hours; after the enzymolysis, the mixed slurry is heated in a boiling water bath at 95 to 100°C for 10 minutes; then the pH of the slurry is adjusted to neutral; finally, the mixed slurry is heated at 4°C Centrifuge at 7000r/min for 20min, take the supernatant as jellyfish hydrolyzate, vacuum freeze-dry, and store at -20~-80℃ for later use;
S2:原料的前处理S2: Pretreatment of raw materials
盐渍海蜇分为海蜇伞和海蜇头,海蜇伞加工前切丝,海蜇头加工前切成大小约为2cm*2cm小块,采用流动水对海蜇进行脱盐至无卤味;Salted jellyfish is divided into jellyfish umbrella and jellyfish head. The jellyfish umbrella is cut into shreds before processing, and the jellyfish head is cut into small pieces with a size of about 2cm*2cm before processing. The jellyfish is desalted with running water until it has no halogen taste;
S3:漂洗:采用纯净水冲洗2~5次;S3: Rinse: use pure water to rinse 2 to 5 times;
S4:热烫减菌:在50~70℃的水中热烫,30~60s;S4: blanching to reduce bacteria: blanching in water at 50-70°C for 30-60s;
S5:以下方式择一进行酸处理抑菌:S5: Choose one of the following methods for acid treatment to inhibit bacteria:
方式1、醋酸溶液浸泡,将海蜇置于醋酸含量为0.03~0.15g/100mL的溶液中浸泡,料液比为1:5~1:20,浸泡时间为3~5min;Method 1. Soak in acetic acid solution. Soak the jellyfish in a solution with an acetic acid content of 0.03-0.15g/100mL. The ratio of solid to liquid is 1:5-1:20, and the soaking time is 3-5 minutes;
方式2、柠檬酸溶液浸泡,将海蜇置于柠檬酸含量为0.05~0.2g/100mL的溶液中进行浸泡,料液比为1:5~1:20,浸泡时间为3~5min;Method 2. Soak in citric acid solution. Soak the jellyfish in a solution with a citric acid content of 0.05-0.2g/100mL. The solid-liquid ratio is 1:5-1:20, and the soaking time is 3-5 minutes;
S6:添加保鲜剂抑菌S6: Add preservatives to inhibit bacteria
将山梨酸钾与S1所得海蜇酶解物按照质量比为1:1进行复配得复配保鲜剂,将所述复配保鲜剂溶解后与S5所得海蜇产品按照添加剂含量/产品含量为1~2g/kg混合包装,封口;Compound potassium sorbate and jellyfish enzymatic hydrolyzate obtained in S1 according to the mass ratio of 1:1 to obtain a compound preservative, dissolve the compound preservative and combine it with the jellyfish product obtained in S5 according to the additive content/product content of 1~ 2g/kg mixed packaging, sealed;
S7:紫外照射灭菌:将步骤S6得到的包装好的产品置于30~40w紫外灯下照射1~3h;S7: Sterilize by ultraviolet irradiation: irradiate the packaged product obtained in step S6 under a 30-40w ultraviolet lamp for 1-3 hours;
S8:贮藏:将产品置于4~10℃中冷藏保存。S8: Storage: Store the product in refrigerated storage at 4-10°C.
其中,针对盐渍海蜇原料,上述步骤S1所述盐渍海蜇加工废弃物冻干粉的制备过程为:海蜇加工过程中被丢弃的部位,包括海蜇胃柱、丝状附属器,或海蜇伞中间厚度超过1cm部位,进行真空冷冻干燥粉碎后得到冻干粉。也就是说,本发明盐渍海蜇加工废弃物主要是海蜇加工过程中经常被丢弃的部位,如:海蜇胃柱、丝状附属器等,或海蜇伞中间厚度超过1cm部位,此处海蜇的脆性较差,韧性大,不适合被加工成海蜇丝,将这些部位进行真空冷冻干燥粉碎后得到冻干粉。Among them, for the salted jellyfish raw material, the preparation process of the salted jellyfish processing waste freeze-dried powder described in the above step S1 is: the discarded parts during the jellyfish processing process, including the jellyfish stomach column, filamentous appendages, or the center of the jellyfish umbrella Parts with a thickness exceeding 1 cm are subjected to vacuum freeze-drying and pulverization to obtain freeze-dried powder. That is to say, the salted jellyfish processing waste of the present invention is mainly the parts that are often discarded in the jellyfish processing process, such as: jellyfish stomach column, filamentous appendages, etc., or the middle thickness of the jellyfish umbrella exceeds 1cm, where the jellyfish is brittle. Poor, strong toughness, not suitable for processing into shredded jellyfish, these parts are subjected to vacuum freeze-drying and crushed to obtain freeze-dried powder.
本发明的技术创新在于首次将栅栏技术应用于新鲜海蜇的保鲜,采用多种栅栏因子结合的方式对新鲜海蜇进行保鲜,保鲜效果更好,海蜇的保存时间更长。同时,由于海蜇原料特殊,含水量极高,目前尚未出现新鲜海蜇低温保鲜的报道,本发明为减少冷冻对海蜇组织造成的损伤,在冷冻前加入梯度乙醇浸泡处理,目的是降低海蜇组织的冻结点,梯度浸泡还可避免组织的大量脱水,经此方法处理的海蜇冷冻流失较少,可较好地保留新鲜海蜇的口感和含水量。The technical innovation of the present invention is that the barrier technology is applied to the preservation of fresh jellyfish for the first time, and the preservation of fresh jellyfish is carried out by combining multiple barrier factors, which has better preservation effect and longer storage time of jellyfish. At the same time, due to the special raw materials of jellyfish and extremely high water content, there has not been any report on low-temperature preservation of fresh jellyfish. In order to reduce the damage caused by freezing to jellyfish tissue, the present invention adds gradient ethanol soaking treatment before freezing, and the purpose is to reduce the freezing of jellyfish tissue. Point, gradient immersion can also avoid a large amount of tissue dehydration, the jellyfish treated by this method has less freezing loss, and can better retain the taste and water content of fresh jellyfish.
对于盐渍海蜇,将漂洗、热烫、酸处理、紫外照射、复配保鲜剂作为栅栏因子,通过多种栅栏因子的结合作用减少添加剂的使用量,并达到延长产品保质期的目的。For salted jellyfish, rinsing, blanching, acid treatment, ultraviolet radiation, and compound preservatives are used as barrier factors, and the combination of multiple barrier factors reduces the amount of additives used and prolongs the shelf life of products.
本发明的技术创新在于将栅栏技术应用于盐渍海蜇的加工工艺中,多种手段的联合使用可有效延长产品保质期。同时,将海蜇加工边角料进行加工利用,制备生物保鲜剂,生物保鲜剂与化学保鲜剂的复配使用可有效抑制海蜇贮藏过程中微生物的生长,同时减少了化学添加剂的使用量,产品食用更加安全。The technical innovation of the invention lies in the application of fence technology to the processing technology of salted jellyfish, and the combined use of multiple means can effectively prolong the shelf life of the product. At the same time, the leftovers of jellyfish processing are processed and utilized to prepare biological preservatives. The compound use of biological preservatives and chemical preservatives can effectively inhibit the growth of microorganisms during jellyfish storage, and at the same time reduce the use of chemical additives, making the product safer to eat .
具体实施方式detailed description
实施例1:Example 1:
1.新鲜海蜇打捞上岸后,迅速按伞、胃柱、肩板、口腕、生殖腺进行分割;1. After the fresh jellyfish is salvaged, it is quickly divided according to the umbrella, stomach column, shoulder plate, mouth wrist and gonad;
2.将分割好的海蜇放入冷水中进行简单的清洗;2. Put the divided jellyfish into cold water for simple cleaning;
3.采用纯净水对海蜇进行冲洗2次;3. Rinse the jellyfish twice with pure water;
4.将冲洗后的海蜇在60℃的热水中热烫60s;4. Blanch the rinsed jellyfish in hot water at 60°C for 60 seconds;
5.将热烫后的海蜇置于醋酸含量为0.1g/100mL的溶液中浸泡,料液比为1:5,浸泡时间为5min;5. Soak the blanched jellyfish in a solution with an acetic acid content of 0.1g/100mL, the ratio of solid to liquid is 1:5, and the soaking time is 5 minutes;
6.将处理后的海蜇分别浸泡在5%、10%、15%、20%的梯度食用酒精溶液中,每个溶液中浸泡30min,料液比为1:5;6. Soak the treated jellyfish in 5%, 10%, 15%, and 20% gradient edible alcohol solutions, soak in each solution for 30 minutes, and the ratio of solid to liquid is 1:5;
7.将抗冻处理后的海蜇连同抗冻液(体积比为20%的食用酒精水溶液)按照质量比为1:1一同包装后放入-18℃低温环境下进行冷冻保藏。7. Pack the antifreeze-treated jellyfish together with the antifreeze solution (20% edible alcohol aqueous solution by volume) according to the mass ratio of 1:1 and put them into a low-temperature environment of -18°C for cryopreservation.
反例1:Counter example 1:
1.新鲜海蜇打捞上岸后,迅速按伞、胃柱、肩板、口腕、生殖腺进行分割;1. After the fresh jellyfish is salvaged, it is quickly divided according to the umbrella, stomach column, shoulder plate, mouth wrist and gonad;
2.将分割好的海蜇放入冷水中进行简单的清洗;2. Put the divided jellyfish into cold water for simple cleaning;
3.采用纯净水对海蜇进行冲洗2次;3. Rinse the jellyfish twice with pure water;
4.将冲洗后的海蜇在60℃的热水中热烫60s;4. Blanch the rinsed jellyfish in hot water at 60°C for 60 seconds;
5.将处理后的海蜇分别浸泡在5%、10%、15%、20%的梯度食用酒精溶液中,每个溶液中浸泡30min,料液比为1:5;5. Soak the treated jellyfish in 5%, 10%, 15%, and 20% gradient edible alcohol solutions, soak in each solution for 30 minutes, and the ratio of solid to liquid is 1:5;
6.将抗冻处理后的海蜇连同抗冻液(体积比为20%的食用酒精水溶液)按照质量比为1:1一同包装后放入-18℃低温环境下进行冷冻保藏。6. Pack the antifreeze-treated jellyfish together with the antifreeze solution (20% edible alcohol aqueous solution by volume) according to the mass ratio of 1:1 and put them into a low temperature environment of -18°C for cryopreservation.
与反例1相比,实例1增加了醋酸溶液的浸泡,采用GB4789.2-2010菌落总数的测定方法测定产品中微生物数量从1000~3000cfu/g减少至30~200cfu/g,并采用贮藏实验研究发现此步骤可有效抑制产品贮藏过程中微生物的增长。Compared with negative example 1, Example 1 increased the immersion in acetic acid solution, and the number of microorganisms in the product was reduced from 1000-3000cfu/g to 30-200cfu/g using the determination method of the total number of bacterial colonies in GB4789.2-2010, and the storage experiment was used to study This step was found to be effective in inhibiting microbial growth during product storage.
实施例2:Example 2:
1.新鲜海蜇打捞上岸后,迅速按伞、胃柱、肩板、口腕、生殖腺进行分割;1. After the fresh jellyfish is salvaged, it is quickly divided according to the umbrella, stomach column, shoulder plate, mouth wrist and gonad;
2.将分割好的海蜇放入冷水中进行简单的清洗;2. Put the divided jellyfish into cold water for simple cleaning;
3.采用纯净水对海蜇进行冲洗2次;3. Rinse the jellyfish twice with pure water;
4.将冲洗后的海蜇在70℃的热水中热烫30s;4. Blanch the rinsed jellyfish in hot water at 70°C for 30 seconds;
5.将热烫后的海蜇置于柠檬酸含量为0.1g/100mL的溶液中进行浸泡,料液比为1:10,浸泡时间为5min;5. Soak the blanched jellyfish in a solution with a citric acid content of 0.1g/100mL, the ratio of solid to liquid is 1:10, and the soaking time is 5 minutes;
6.将处理后的海蜇分别浸泡在5%、10%、15%、20%的梯度食用酒精溶液中,每个溶液中浸泡30min,料液比为1:5;6. Soak the treated jellyfish in 5%, 10%, 15%, and 20% gradient edible alcohol solutions, soak in each solution for 30 minutes, and the ratio of solid to liquid is 1:5;
7.将抗冻处理后的海蜇连同抗冻液(体积比为20%的食用酒精水溶液)按照质量比为1:1一同包装后放入-18℃低温环境下进行冷冻保藏。7. Pack the antifreeze-treated jellyfish together with the antifreeze solution (20% edible alcohol aqueous solution by volume) according to the mass ratio of 1:1 and put them into a low-temperature environment of -18°C for cryopreservation.
反例2:Counter example 2:
1.新鲜海蜇打捞上岸后,迅速按伞、胃柱、肩板、口腕、生殖腺进行分割;1. After the fresh jellyfish is salvaged, it is quickly divided according to the umbrella, stomach column, shoulder plate, mouth wrist and gonad;
2.将分割好的海蜇放入冷水中进行简单的清洗;2. Put the divided jellyfish into cold water for simple cleaning;
3.采用纯净水对海蜇进行冲洗2次;3. Rinse the jellyfish twice with pure water;
4.将冲洗后的海蜇在70℃的热水中热烫30s;4. Blanch the rinsed jellyfish in hot water at 70°C for 30 seconds;
5.将热烫后的海蜇置于柠檬酸含量为0.1g/100mL的溶液中进行浸泡,料液比为1:10,浸泡时间为5min;5. Soak the blanched jellyfish in a solution with a citric acid content of 0.1g/100mL, the ratio of solid to liquid is 1:10, and the soaking time is 5 minutes;
6.将处理后的海蜇包装后放入-18℃低温环境下进行冷冻保藏。6. Pack the processed jellyfish and put it into a low-temperature environment of -18°C for frozen storage.
与反例2相比,实例2增加了梯度食用酒精浸泡,采用降温曲线测定的方法得到海蜇的冻结点从0℃减少到-3℃~-15℃,冻结点的降低有利于减少低温及冻融过程对组织造成的破坏。Compared with the negative example 2, example 2 increases the gradient edible alcohol immersion, and the freezing point of jellyfish is reduced from 0°C to -3°C to -15°C by using the method of temperature-dropping curve measurement. The reduction of freezing point is beneficial to reduce low temperature and freeze-thaw. Damage caused by the process to the organization.
实施例3:Example 3:
1.对海蜇加工废弃物(海蜇废弃物主要是海蜇加工过程中经常被丢弃的部位,如:海蜇胃柱、丝状附属器等,或海蜇伞中间厚度超过1cm部位,此处海蜇的脆性较差,韧性大,不适合被加工成海蜇丝)进行冻干,粉碎;1. Jellyfish processing waste (jellyfish waste is mainly the parts that are often discarded during jellyfish processing, such as: jellyfish stomach column, filamentous appendages, etc., or the part where the thickness of the jellyfish umbrella exceeds 1cm, where the jellyfish is more brittle Poor, strong toughness, not suitable for being processed into jellyfish shreds) freeze-dried and crushed;
2.采用胰蛋白酶(最适pH=8.0,最适温度为37℃)对盐渍海蜇加工废弃物冻干粉进行酶解,酶解条件为:按照海蜇冻干粉质量30倍比例加入去离子水,振荡混合,然后用6mol/L HCl和6mol/L NaOH调节混合浆液pH至胰蛋白酶最适pH8.0,在胰蛋白酶最适温度(37℃)下保温10min后,根据海蜇冻干粉质量以每克冻干粉加入3000U酶活力单位加入胰蛋白酶进行酶解反应,反应过程中用酸和碱调节pH始终在最适pH8.0,酶解时间为3h;酶解结束后,混合浆液在100℃沸水浴中加热10min;再调节浆液pH至中性;最后将混合浆液在4℃下7000r/min离心20min,取上清液为海蜇酶解物,真空冷冻干燥后,-80℃保存备用;2. Use trypsin (optimum pH = 8.0, optimum temperature 37°C) to enzymatically hydrolyze the freeze-dried powder of salted jellyfish processing waste. The enzymatic hydrolysis condition is: add deionization in proportion to 30 times the mass of jellyfish freeze-dried powder water, shake and mix, and then use 6mol/L HCl and 6mol/L NaOH to adjust the pH of the mixed slurry to the optimum pH of trypsin at 8.0, and after incubating at the optimum temperature of trypsin (37°C) for 10min, according to the quality of jellyfish freeze-dried powder Add 3000U enzyme activity units per gram of freeze-dried powder and add trypsin for enzymolysis reaction. During the reaction process, use acid and alkali to adjust the pH to always be at the optimum pH 8.0, and the enzymolysis time is 3 hours; after the enzymolysis, the mixed slurry is in Heat in a boiling water bath at 100°C for 10 minutes; then adjust the pH of the slurry to neutral; finally centrifuge the mixed slurry at 7000r/min at 4°C for 20 minutes, take the supernatant as jellyfish hydrolyzate, vacuum freeze-dry, and store at -80°C for later use ;
3.将酶解物冻干粉与山梨酸钾按照1:1混合即得到复配保鲜剂;3. Mix the freeze-dried powder of the enzymatic hydrolyzate and potassium sorbate at a ratio of 1:1 to obtain a compound preservative;
4.对盐渍海蜇原料按海蜇伞和海蜇头进行分割,海蜇伞加工前切丝,海蜇头加工前切成大小约为2cm*2cm小块,采用流动水对海蜇进行脱盐至无卤味;4. Divide the raw materials of salted jellyfish according to jellyfish umbrella and jellyfish head, shred the jellyfish umbrella before processing, and cut the jellyfish head into small pieces with a size of about 2cm*2cm before processing, and use running water to desalt the jellyfish until it has no halogen taste;
5.采用纯净水对脱盐海蜇进行冲洗,冲洗2次;5. Rinse the desalted jellyfish with pure water for 2 times;
6.将冲洗后的海蜇在60℃的热水中热烫30s;6. Blanch the rinsed jellyfish in hot water at 60°C for 30 seconds;
7.将热烫后的海蜇置于醋酸含量为0.1g/100mL的溶液中浸泡,料液比为1:5,浸泡时间为5min;7. Soak the blanched jellyfish in a solution with an acetic acid content of 0.1g/100mL, the ratio of solid to liquid is 1:5, and the soaking time is 5 minutes;
8.使用纯净水将复配保鲜剂溶解后与得到的海蜇产品按照添加剂含量/产品含量为1g/kg混合包装,常压封口;8. Use purified water to dissolve the compound preservative and mix with the obtained jellyfish product according to the additive content/product content of 1g/kg, and seal under normal pressure;
9.对产品进行紫外照射杀菌,紫外灯功率为30w,杀菌时间为1h;9. Sterilize the product by ultraviolet irradiation, the power of the ultraviolet lamp is 30w, and the sterilization time is 1h;
10.将产品置于冷藏(4℃)环境下保存。10. Store the product in a refrigerated (4°C) environment.
反例3:Counter example 3:
1.对盐渍海蜇原料按海蜇伞和海蜇头进行分割,海蜇伞加工前切丝,海蜇头加工前切成大小约为2cm*2cm小块,采用流动水对海蜇进行脱盐至无卤味;1. Divide the raw materials of salted jellyfish according to jellyfish umbrella and jellyfish head, shred the jellyfish umbrella before processing, and cut the jellyfish head into small pieces with a size of about 2cm*2cm before processing, and use running water to desalt the jellyfish until it has no halogen taste;
2.采用纯净水对脱盐海蜇进行冲洗,冲洗2次;2. Rinse the desalted jellyfish with pure water for 2 times;
3.将冲洗后的海蜇在60℃的热水中热烫30s;3. Blanch the rinsed jellyfish in hot water at 60°C for 30 seconds;
4.将热烫后的海蜇置于醋酸含量为0.1g/100mL的溶液中浸泡,料液比为1:5,浸泡时间为5min;4. Soak the blanched jellyfish in a solution with an acetic acid content of 0.1g/100mL, the ratio of solid to liquid is 1:5, and the soaking time is 5 minutes;
5.使用纯净水将山梨酸钾溶解后按照添加剂含量/产品含量为1g/kg混合包装,常压封口。5. Potassium sorbate is dissolved in purified water, then mixed and packaged according to the additive content/product content of 1g/kg, and sealed under normal pressure.
6.对产品进行紫外照射杀菌,紫外灯功率为30w,杀菌时间为1h;6. Sterilize the product by ultraviolet irradiation, the power of the ultraviolet lamp is 30w, and the sterilization time is 1h;
7.将产品置于冷藏(4℃)环境下保存。7. Store the product in a refrigerated (4°C) environment.
与反例3相比,实例3采用复配保鲜剂对海蜇进行保鲜,采用GB4789.2的方法测定产品中微生物水平均符合即食海蜇出厂检验标准,但实施例3中的山梨酸钾的使用量减少了50%,提高了产品的食用安全性。Compared with negative example 3, example 3 adopts compound antistaling agent to keep jellyfish fresh, and adopts the method of GB4789.2 to measure the level of microorganisms in the product, which meets the factory inspection standard of instant jellyfish, but the usage of potassium sorbate in example 3 is reduced Increased by 50%, improving the food safety of the product.
实施例4:Example 4:
1.对海蜇加工废弃物(海蜇废弃物主要是海蜇加工过程中经常被丢弃的部位,如:海蜇胃柱、丝状附属器等,或海蜇伞中间厚度超过1cm部位,此处海蜇的脆性较差,韧性大,不适合被加工成海蜇丝)进行冻干,粉碎;1. Jellyfish processing waste (jellyfish waste is mainly the parts that are often discarded during jellyfish processing, such as: jellyfish stomach column, filamentous appendages, etc., or the part where the thickness of the jellyfish umbrella exceeds 1cm, where the jellyfish is more brittle Poor, strong toughness, not suitable for being processed into jellyfish shreds) freeze-dried and crushed;
2.采用胰蛋白酶(最适pH=8.0,最适温度为37℃)对盐渍海蜇加工废弃物冻干粉进行酶解,酶解条件为:按照海蜇冻干粉质量40倍比例加入去离子水,振荡混合,然后用6mol/L HCl和6mol/L NaOH调节混合浆液pH至胰蛋白酶最适pH8.0,在胰蛋白酶最适温度(37℃)下保温10min后,根据海蜇冻干粉质量以每克冻干粉加入3000U酶活力单位加入胰蛋白酶进行酶解反应,反应过程中用酸和碱调节pH始终在最适pH8.0,酶解时间为3h;酶解结束后,混合浆液在100℃沸水浴中加热10min;再调节浆液pH至中性;最后将混合浆液在4℃下7000r/min离心20min,取上清液为海蜇酶解物,真空冷冻干燥后,-80℃保存备用;2. Use trypsin (optimum pH = 8.0, optimum temperature 37°C) to enzymatically hydrolyze the freeze-dried powder of salted jellyfish processing waste. The enzymatic hydrolysis condition is: add deionization in proportion to 40 times the mass of jellyfish freeze-dried powder water, shake and mix, and then use 6mol/L HCl and 6mol/L NaOH to adjust the pH of the mixed slurry to the optimum pH of trypsin at 8.0, and after incubating at the optimum temperature of trypsin (37°C) for 10min, according to the quality of jellyfish freeze-dried powder Add 3000U enzyme activity units per gram of freeze-dried powder and add trypsin for enzymolysis reaction. During the reaction process, use acid and alkali to adjust the pH to always be at the optimum pH 8.0, and the enzymolysis time is 3 hours; after the enzymolysis, the mixed slurry is in Heat in a boiling water bath at 100°C for 10 minutes; then adjust the pH of the slurry to neutral; finally centrifuge the mixed slurry at 7000r/min at 4°C for 20 minutes, take the supernatant as jellyfish hydrolyzate, vacuum freeze-dry, and store at -80°C for later use ;
3.将酶解物冻干粉与山梨酸钾按照1:1混合即得到复配保鲜剂;3. Mix the freeze-dried powder of the enzymatic hydrolyzate and potassium sorbate at a ratio of 1:1 to obtain a compound preservative;
4.对盐渍海蜇原料按海蜇伞和海蜇头进行分割,海蜇伞加工前切丝,海蜇头加工前切成大小约为2cm*2cm小块,采用流动水对海蜇进行脱盐至无卤味;4. Divide the raw materials of salted jellyfish according to jellyfish umbrella and jellyfish head, shred the jellyfish umbrella before processing, and cut the jellyfish head into small pieces with a size of about 2cm*2cm before processing, and use running water to desalt the jellyfish until it has no halogen taste;
5.采用纯净水对脱盐海蜇进行冲洗,冲洗2次;5. Rinse the desalted jellyfish with pure water for 2 times;
6.将冲洗后的海蜇在60℃的热水中热烫60s;6. Blanch the rinsed jellyfish in hot water at 60°C for 60 seconds;
7.将热烫后的海蜇置于柠檬酸含量为0.1g/100mL的溶液中进行浸泡,料液比为1:10,浸泡时间为5min;7. Soak the blanched jellyfish in a solution with a citric acid content of 0.1g/100mL, the ratio of solid to liquid is 1:10, and the soaking time is 5 minutes;
8.使用纯净水将复配保鲜剂溶解后与得到的海蜇产品按照添加剂含量/产品含量为1g/kg混合包装,常压封口;;8. Use purified water to dissolve the compound preservative and mix it with the obtained jellyfish product according to the additive content/product content of 1g/kg, and seal it under normal pressure;
9.对产品进行紫外照射杀菌,紫外灯功率为40w,杀菌时间为3h;9. Sterilize the product by ultraviolet irradiation, the power of the ultraviolet lamp is 40w, and the sterilization time is 3h;
10.将产品置于冷藏(4℃)环境下保存。10. Store the product in a refrigerated (4°C) environment.
反例4:Counter example 4:
1.对海蜇加工废弃物(海蜇废弃物主要是海蜇加工过程中经常被丢弃的部位,如:海蜇胃柱、丝状附属器等,或海蜇伞中间厚度超过1cm部位,此处海蜇的脆性较差,韧性大,不适合被加工成海蜇丝)进行冻干,粉碎;1. Jellyfish processing waste (jellyfish waste is mainly the parts that are often discarded during jellyfish processing, such as: jellyfish stomach column, filamentous appendages, etc., or the part where the thickness of the jellyfish umbrella exceeds 1cm, where the jellyfish is more brittle Poor, strong toughness, not suitable for being processed into jellyfish shreds) freeze-dried and crushed;
2.采用胰蛋白酶(最适pH=8.0,最适温度为37℃)对盐渍海蜇加工废弃物冻干粉进行酶解,酶解条件为:按照海蜇冻干粉质量40倍比例加入去离子水,振荡混合,然后用6mol/L HCl和6mol/L NaOH调节混合浆液pH至胰蛋白酶最适pH8.0,在胰蛋白酶最适温度(37℃)下保温10min后,根据海蜇冻干粉质量以每克冻干粉加入3000U酶活力单位加入胰蛋白酶进行酶解反应,反应过程中用酸和碱调节pH始终在最适pH8.0,酶解时间为3h;酶解结束后,混合浆液在100℃沸水浴中加热10min;再调节浆液pH至中性;最后将混合浆液在4℃下7000r/min离心20min,取上清液为海蜇酶解物,真空冷冻干燥后,-80℃保存备用;2. Use trypsin (optimum pH = 8.0, optimum temperature 37°C) to enzymatically hydrolyze the freeze-dried powder of salted jellyfish processing waste. The enzymatic hydrolysis condition is: add deionization in proportion to 40 times the mass of jellyfish freeze-dried powder water, shake and mix, and then use 6mol/L HCl and 6mol/L NaOH to adjust the pH of the mixed slurry to the optimum pH of trypsin at 8.0, and after incubating at the optimum temperature of trypsin (37°C) for 10min, according to the quality of jellyfish freeze-dried powder Add 3000U enzyme activity units per gram of freeze-dried powder and add trypsin for enzymolysis reaction. During the reaction process, use acid and alkali to adjust the pH to always be at the optimum pH 8.0, and the enzymolysis time is 3 hours; after the enzymolysis, the mixed slurry is in Heat in a boiling water bath at 100°C for 10 minutes; then adjust the pH of the slurry to neutral; finally centrifuge the mixed slurry at 7000r/min at 4°C for 20 minutes, take the supernatant as jellyfish hydrolyzate, vacuum freeze-dry, and store at -80°C for later use ;
3.将酶解物冻干粉与山梨酸钾按照1:1混合即得到复配保鲜剂;3. Mix the freeze-dried powder of the enzymatic hydrolyzate and potassium sorbate at a ratio of 1:1 to obtain a compound preservative;
4.对盐渍海蜇原料按海蜇伞和海蜇头进行分割,海蜇伞加工前切丝,海蜇头加工前切成大小约为2cm*2cm小块,采用流动水对海蜇进行脱盐至无卤味;4. Divide the raw materials of salted jellyfish according to jellyfish umbrella and jellyfish head, shred the jellyfish umbrella before processing, and cut the jellyfish head into small pieces with a size of about 2cm*2cm before processing, and use running water to desalt the jellyfish until it has no halogen taste;
5.采用纯净水对脱盐海蜇进行冲洗,冲洗2次;5. Rinse the desalted jellyfish with pure water for 2 times;
6.将冲洗后的海蜇在60℃的热水中热烫60s;6. Blanch the rinsed jellyfish in hot water at 60°C for 60 seconds;
7.将热烫后的海蜇置于柠檬酸含量为0.1g/100mL的溶液中进行浸泡,料液比为1:10,浸泡时间为5min;7. Soak the blanched jellyfish in a solution with a citric acid content of 0.1g/100mL, the ratio of solid to liquid is 1:10, and the soaking time is 5 minutes;
8.使用纯净水将复配保鲜剂溶解后与得到的海蜇产品按照添加剂含量/产品含量为1g/kg混合包装,常压封口;;8. Use pure water to dissolve the compound preservative and mix with the obtained jellyfish product according to the additive content/product content of 1g/kg, and seal under normal pressure;
9.将产品置于冷藏(4℃)环境下保存。9. Store the product in a refrigerated (4°C) environment.
与反例4相比,实施例4在使用其他栅栏技术的基础上采用紫外照射对产品进行灭菌,产品中的微生物数量可达到30cfu/g以下。Compared with Counter-Example 4, Example 4 uses ultraviolet radiation to sterilize the product on the basis of using other barrier technologies, and the number of microorganisms in the product can reach below 30cfu/g.
本发明的技术创新在于首次将栅栏技术应用于新鲜海蜇的保鲜,采用多种栅栏因子结合的方式对新鲜海蜇进行保鲜,海蜇样品贮藏前的菌落总数可减少至原来的5%~20%,海蜇的保存时间可延长至6~9个月。同时,由于海蜇原料特殊,含水量极高,目前尚未出现新鲜海蜇低温保鲜的报道,本发明为减少冷冻对海蜇组织造成的损伤,在冷冻前加入梯度乙醇浸泡处理,目的是降低海蜇组织的冻结点,梯度浸泡还可避免组织的大量脱水,经此方法处理的海蜇冷冻流失较少,可较好地保留新鲜海蜇的口感和含水量。The technical innovation of the present invention is that the barrier technology is applied to the preservation of fresh jellyfish for the first time, and the fresh jellyfish is preserved by adopting a combination of various barrier factors. The storage time can be extended to 6-9 months. At the same time, due to the special raw materials of jellyfish and extremely high water content, there has not been any report on low-temperature preservation of fresh jellyfish. In order to reduce the damage caused by freezing to jellyfish tissue, the present invention adds gradient ethanol soaking treatment before freezing, and the purpose is to reduce the freezing of jellyfish tissue. Point, gradient immersion can also avoid a large amount of tissue dehydration, the jellyfish treated by this method has less freezing loss, and can better retain the taste and water content of fresh jellyfish.
对于盐渍海蜇,本发明的技术创新在于将海蜇加工边角料进行加工利用,制备生物保鲜剂,生物保鲜剂与化学保鲜剂的复配使用可有效抑制海蜇贮藏过程中微生物的生长,同时,多种栅栏因子的协同作用减少了化学添加剂的使用量,产品食用更加安全。For salted jellyfish, the technical innovation of the present invention is to process and utilize jellyfish processing leftovers to prepare biological preservatives. The compound use of biological preservatives and chemical preservatives can effectively inhibit the growth of microorganisms during jellyfish storage. At the same time, various The synergistic effect of barrier factors reduces the amount of chemical additives used, and the product is safer to eat.
以上所述,仅为本发明较佳的具体实施方式,但本发明的保护范围并不局限于此,任何熟悉本技术领域的技术人员在本发明披露的技术范围内,根据本发明的技术方案及其发明构思加以等同替换或改变,都应涵盖在本发明的保护范围之内。The above is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Anyone familiar with the technical field within the technical scope disclosed in the present invention, according to the technical solution of the present invention Any equivalent replacement or change of the inventive concepts thereof shall fall within the protection scope of the present invention.
Claims (1)
- A kind of 1. method that jelly-fish fresh-keeping and processing are carried out using hurdle technology, it is characterised in that for fresh jellyfish raw material, bag Include following steps:S1:Pre-treatment:Fresh jellyfish, split by umbrella, stomach post, scapulet, oral arm, gonad;S2:Rinsing:Pure water rinses 2~5 times;S3:Blanching bacteria reducing:The blanching in 60~80 DEG C of hot water, blanching treatment time are:30~60s;S4:It is antibacterial that in the following manner selects a carry out acid treatment:Mode 1:Acetum is soaked, and jellyfish is placed in the solution that acetic acid content is 0.03~0.15g/100mL and soaked, feed liquid Than for 1:5~1:20, soak time is 3~5min;Mode 2:Citric acid solution is soaked, and jellyfish is placed in the solution that citric acid content is 0.05~0.2g/100mL and soaked Bubble, solid-liquid ratio 1:5~1:20, soak time is 3~5min;S5:The freeze proof processing of concentration gradientJellyfish is separately immersed in the gradient edible alcohol solution that volume ratio is 5%, 10%, 15%, 20%, soak time is 30min, solid-liquid ratio 1:2~1:10;S6:StorageAccording to mass ratio it is 1 by edible alcohol water solution that the jellyfish after freeze proof processing and volume ratio are 20%:After 1 is hybrid packed It is put into -10~-18 DEG C of low temperature environments and carries out preservation.
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