CN114478687A - Hippocampus protein crude extraction process - Google Patents
Hippocampus protein crude extraction process Download PDFInfo
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- CN114478687A CN114478687A CN202210337535.2A CN202210337535A CN114478687A CN 114478687 A CN114478687 A CN 114478687A CN 202210337535 A CN202210337535 A CN 202210337535A CN 114478687 A CN114478687 A CN 114478687A
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- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 63
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 63
- 238000000605 extraction Methods 0.000 title claims abstract description 35
- 210000001320 hippocampus Anatomy 0.000 title claims description 54
- 239000000843 powder Substances 0.000 claims abstract description 85
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 75
- 239000000243 solution Substances 0.000 claims abstract description 65
- 101000831205 Danio rerio Dynein axonemal assembly factor 11 Proteins 0.000 claims abstract description 52
- 102100024282 Dynein axonemal assembly factor 11 Human genes 0.000 claims abstract description 52
- 101000831210 Homo sapiens Dynein axonemal assembly factor 11 Proteins 0.000 claims abstract description 52
- 241001559542 Hippocampus hippocampus Species 0.000 claims abstract description 50
- 230000000971 hippocampal effect Effects 0.000 claims abstract description 50
- 239000011259 mixed solution Substances 0.000 claims abstract description 34
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 27
- 238000001035 drying Methods 0.000 claims abstract description 26
- 238000002137 ultrasound extraction Methods 0.000 claims abstract description 21
- 238000003756 stirring Methods 0.000 claims abstract description 18
- 235000019750 Crude protein Nutrition 0.000 claims abstract description 9
- 238000004140 cleaning Methods 0.000 claims abstract description 9
- 238000001914 filtration Methods 0.000 claims abstract description 9
- 238000000227 grinding Methods 0.000 claims abstract description 9
- 230000007935 neutral effect Effects 0.000 claims abstract description 9
- 230000001954 sterilising effect Effects 0.000 claims abstract description 9
- 239000012535 impurity Substances 0.000 claims abstract description 4
- 238000006555 catalytic reaction Methods 0.000 claims description 8
- 238000002425 crystallisation Methods 0.000 claims description 8
- 230000008025 crystallization Effects 0.000 claims description 8
- 238000005238 degreasing Methods 0.000 claims description 8
- 238000002156 mixing Methods 0.000 claims description 8
- 239000002002 slurry Substances 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 2
- 235000018102 proteins Nutrition 0.000 abstract description 53
- 235000021245 dietary protein Nutrition 0.000 abstract description 2
- 238000010298 pulverizing process Methods 0.000 abstract description 2
- 239000003960 organic solvent Substances 0.000 description 15
- 238000002791 soaking Methods 0.000 description 4
- 239000003814 drug Substances 0.000 description 3
- 239000004519 grease Substances 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000000857 drug effect Effects 0.000 description 2
- 238000005728 strengthening Methods 0.000 description 2
- 206010011224 Cough Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 238000007696 Kjeldahl method Methods 0.000 description 1
- 238000000944 Soxhlet extraction Methods 0.000 description 1
- 238000007792 addition Methods 0.000 description 1
- 208000006673 asthma Diseases 0.000 description 1
- 230000003467 diminishing effect Effects 0.000 description 1
- 238000001599 direct drying Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 229910017053 inorganic salt Inorganic materials 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 229940126680 traditional chinese medicines Drugs 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
- C07K1/303—Extraction; Separation; Purification by precipitation by salting out
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention discloses a crude extraction process of hippocampal protein, which relates to the field of extraction of food protein and comprises the following steps: pretreating the sea horse, cleaning impurities, sterilizing, drying, pulverizing, grinding, adding absolute ethyl alcohol, placing the mixed sea horse powder in an ultrasonic extraction device, centrifuging the solution after ultrasonic extraction by using a centrifugal machine, filtering, adding NaOH solution, fully stirring, adjusting the pH value of the extracting solution to be neutral, carrying out low-temperature concentration on the crude protein extracting solution, adding absolute ethyl alcohol of the same amount of a body machine into the concentrated solution, slowly adding NaCI after uniformly stirring to obtain a mixed solution of sea horse protein, removing the mixed solution through a filter, and drying the obtained sea horse protein powder.
Description
Technical Field
The invention relates to the field of extraction of food protein, in particular to a crude extraction process of hippocampal protein.
Background
One of the famous and precious traditional Chinese medicines of the hippocampus has high important use value, and the hippocampus has a long history like medicines, and is mainly used for tonifying kidney and strengthening yang, strengthening heart and tonifying spleen, relieving cough and asthma, diminishing inflammation and relieving pain and the like.
However, the existing hippocampus is generally taken as a medicine in a wine soaking mode, so that the effective components in the hippocampus cannot effectively play a role, the wine soaking mode has long soaking time and greatly reduced medicine effect, and a large number of people do not drink the wine, so that the utilization rate of the hippocampus is greatly reduced in the wine soaking mode of the hippocampus.
Disclosure of Invention
In order to overcome the technical problem of low drug effect utilization rate of the hippocampus in the prior art, the invention provides a crude extraction process of hippocampus protein, which is used for extracting hippocampus protein, improving the utilization rate of the hippocampus and enhancing drug effect.
The technical scheme adopted by the invention for solving the technical problems is as follows: a crude extraction process of hippocampal protein comprises the following steps:
(1) pretreatment of the hippocampus: cleaning the sea horse to remove impurities, sterilizing, drying, pulverizing, and grinding to obtain sea horse powder.
(2) Degreasing: adding absolute ethyl alcohol which accounts for 3-5 times of the mass of the dry powder of the sea horse powder into the sea horse powder after the step (1), uniformly mixing, then placing the sea horse powder mixed with the slurry into an ultrasonic extraction device, setting the ultrasonic frequency to be 25KHz-35KHz, setting the temperature in the extraction device to be 40-45 ℃, centrifuging the solution after the ultrasonic extraction by using a centrifugal machine, and then filtering to obtain the degreased sea horse powder.
(3) Primary extraction: drying the degreased hippocampus powder obtained in the step (2) at low temperature, adding NaOH solution with the concentration of 0.05-0.08mol/L into the dried hippocampus powder, fully stirring, and adding NaOH solution according to the proportion that the hippocampus powder/NaOH solution is equal to 1: 10-20g/mol, and placing the stirred and mixed solution into an ultrasonic reactor for ultrasonic catalysis for 3-5min to obtain a primary extracting solution of the hippocampal protein.
(4) Separating out and extracting: and (3) adjusting the pH value of the crude hippocampal protein extract obtained in the step (3) to be neutral, carrying out low-temperature concentration on the crude protein extract, stopping concentration when the solution is concentrated to the beginning of crystallization, adding absolute ethyl alcohol with the same amount of an organic solvent into the concentrated solution, stirring uniformly, slowly adding NaCI until NaCI is not dissolved, adding absolute ethyl alcohol with 1/5 organic solvent, and dissolving excessive NaCI to obtain a mixed solution of the hippocampal protein.
(5) And (4) passing the mixed solution of the hippocampal protein in the step (4) through a filter, removing the mixed solution, and drying the obtained hippocampal protein powder.
Further, the water content of the dry hippocampus powder in the step (1) is lower than 5%.
Furthermore, the ultrasonic power of the ultrasonic extraction device is 25KHz-35 KHz.
Further, the rotating speed of the centrifugal machine is 3000-.
The invention has the beneficial effects that:
(1) the invention uses the ultrasonic extraction method to replace the traditional extraction method to separate the grease, mainly utilizes the penetrability of the ultrasonic and the high-frequency principle to enable the grease to be dissociated from the hippocampus powder, and the grease and partial organic matters are dissolved in absolute ethyl alcohol, thereby being beneficial to improving the purity of the hippocampus protein in the next extraction step;
(2) the present invention adopts organic solvent and inorganic salt precipitation mode to precipitate hippocampal protein in solution, so that the impurity is still remained in the solution to precipitate hippocampal protein and raise the purity of hippocampal protein.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Example 1
A crude extraction process of hippocampal protein comprises the following steps:
(1) pretreatment of the hippocampus: cleaning the sea horse, sterilizing, drying, crushing and grinding to obtain sea horse powder with water content lower than 5%.
(2) Degreasing: adding absolute ethyl alcohol which accounts for 3 times of the mass of the dry powder of the sea horse powder into the sea horse powder after the step (1), uniformly mixing, then placing the sea horse powder mixed with the slurry into an ultrasonic extraction device, wherein the ultrasonic frequency is 25KHz, the temperature in the extraction device is set to be 40 ℃, centrifuging the solution after ultrasonic extraction by using a centrifugal machine, and then filtering to obtain the degreased sea horse powder.
(3) Primary extraction: drying the degreased hippocampus powder obtained in the step (2) at low temperature, adding NaOH solution with the concentration of 0.05-0.08mol/L into the dried hippocampus powder, fully stirring, and adding NaOH solution according to the proportion that the hippocampus powder/NaOH solution is equal to 1: 10g/mol, and placing the stirred and mixed solution into an ultrasonic reactor for ultrasonic catalysis for 3-5min to obtain a primary extracting solution of the hippocampus protein.
(4) Separating out and extracting: and (3) adjusting the pH value of the crude hippocampal protein extract obtained in the step (3) to be neutral, carrying out low-temperature concentration on the crude protein extract, stopping concentration when the solution is concentrated to the beginning of crystallization, adding absolute ethyl alcohol with the same amount of an organic solvent into the concentrated solution, stirring uniformly, slowly adding NaCI until NaCI is not dissolved, adding absolute ethyl alcohol with 1/5 organic solvent, and dissolving excessive NaCI to obtain a mixed solution of the hippocampal protein.
(5) And (4) passing the mixed solution of the hippocampal protein in the step (4) through a filter, removing the mixed solution, and drying the obtained hippocampal protein powder.
Example 2
A crude extraction process of hippocampal protein comprises the following steps:
(1) pretreatment of the hippocampus: cleaning the sea horse, sterilizing, drying, crushing and grinding to obtain sea horse powder with water content lower than 5%.
(2) Degreasing: adding absolute ethyl alcohol which accounts for 3 times of the mass of the dry powder of the sea horse powder into the sea horse powder after the step (1), uniformly mixing, then placing the sea horse powder mixed with the slurry into an ultrasonic extraction device, wherein the ultrasonic frequency is 25KHz, the temperature in the extraction device is set to be 40 ℃, centrifuging the solution after ultrasonic extraction by using a centrifugal machine, and then filtering to obtain the degreased sea horse powder.
(3) Primary extraction: drying the degreased hippocampus powder obtained in the step (2) at low temperature, adding NaOH solution with the concentration of 0.05-0.08mol/L into the dried hippocampus powder, fully stirring, and adding NaOH solution according to the proportion that the hippocampus powder/NaOH solution is equal to 1: 20g/mol, and placing the stirred and mixed solution into an ultrasonic reactor for ultrasonic catalysis for 3-5min to obtain a primary extracting solution of the hippocampus protein.
(4) Separating out and extracting: and (3) adjusting the pH value of the crude hippocampal protein extract obtained in the step (3) to be neutral, carrying out low-temperature concentration on the crude protein extract, stopping concentration when the solution is concentrated to the beginning of crystallization, adding absolute ethyl alcohol with the same amount of an organic solvent into the concentrated solution, stirring uniformly, slowly adding NaCI until NaCI is not dissolved, adding absolute ethyl alcohol with 1/5 organic solvent, and dissolving excessive NaCI to obtain a mixed solution of the hippocampal protein.
(5) And (4) passing the mixed solution of the hippocampal protein in the step (4) through a filter, removing the mixed solution, and drying the obtained hippocampal protein powder.
Example 3
A crude extraction process of hippocampal protein comprises the following steps:
(1) pretreatment of hippocampus: cleaning the sea horse, sterilizing, drying, crushing and grinding to obtain sea horse powder with water content lower than 5%.
(2) Degreasing: adding absolute ethyl alcohol which accounts for 5 times of the mass of the dry powder of the sea horse powder into the sea horse powder after the step (1), uniformly mixing, then placing the sea horse powder mixed with the slurry into an ultrasonic extraction device, wherein the ultrasonic frequency is 25KHz, the temperature in the extraction device is set to be 40 ℃, centrifuging the solution after ultrasonic extraction by using a centrifugal machine, and then filtering to obtain the degreased sea horse powder.
(3) Primary extraction: drying the degreased hippocampus powder obtained in the step (2) at low temperature, adding NaOH solution with the concentration of 0.05-0.08mol/L into the dried hippocampus powder, fully stirring, and adding NaOH solution according to the proportion that the hippocampus powder/NaOH solution is equal to 1: 20g/mol, and placing the stirred and mixed solution into an ultrasonic reactor for ultrasonic catalysis for 3-5min to obtain a primary extracting solution of the hippocampus protein.
(4) Separating out and extracting: and (3) adjusting the pH value of the crude hippocampal protein extract obtained in the step (3) to be neutral, carrying out low-temperature concentration on the crude protein extract, stopping concentration when the solution is concentrated to the beginning of crystallization, adding absolute ethyl alcohol with the same amount of an organic solvent into the concentrated solution, stirring uniformly, slowly adding NaCI until NaCI is not dissolved, adding absolute ethyl alcohol with 1/5 organic solvent, and dissolving excessive NaCI to obtain a mixed solution of the hippocampal protein.
(5) And (4) passing the mixed solution of the hippocampal protein in the step (4) through a filter, removing the mixed solution, and drying the obtained hippocampal protein powder.
Example 4
A crude extraction process of hippocampal protein comprises the following steps:
(1) pretreatment of the hippocampus: cleaning the sea horse, sterilizing, drying, crushing and grinding to obtain sea horse powder with water content lower than 5%.
(2) Degreasing: adding absolute ethyl alcohol which accounts for 5 times of the mass of the dry powder of the sea horse powder into the sea horse powder after the step (1), uniformly mixing, then placing the sea horse powder mixed with the slurry into an ultrasonic extraction device, setting the ultrasonic frequency to be 35KHz, setting the temperature in the extraction device to be 40 ℃, centrifuging the solution after ultrasonic extraction by using a centrifugal machine, and then filtering to obtain the degreased sea horse powder.
(3) Primary extraction: drying the degreased hippocampus powder obtained in the step (2) at low temperature, adding NaOH solution with the concentration of 0.05-0.08mol/L into the dried hippocampus powder, fully stirring, and adding NaOH solution according to the proportion that the hippocampus powder/NaOH solution is equal to 1: 20g/mol, and placing the stirred and mixed solution into an ultrasonic reactor for ultrasonic catalysis for 3-5min to obtain a primary extracting solution of the hippocampus protein.
(4) Separating out and extracting: and (3) adjusting the pH value of the crude hippocampal protein extract obtained in the step (3) to be neutral, carrying out low-temperature concentration on the crude protein extract, stopping concentration when the solution is concentrated to the beginning of crystallization, adding absolute ethyl alcohol with the same amount of an organic solvent into the concentrated solution, stirring uniformly, slowly adding NaCI until NaCI is not dissolved, adding absolute ethyl alcohol with 1/5 organic solvent, and dissolving excessive NaCI to obtain a mixed solution of the hippocampal protein.
(5) And (4) passing the mixed solution of the hippocampal protein in the step (4) through a filter, removing the mixed solution, and drying the obtained hippocampal protein powder.
Example 5
A crude extraction process of hippocampal protein comprises the following steps:
(1) pretreatment of hippocampus: cleaning the sea horse, sterilizing, drying, crushing and grinding to obtain sea horse powder with water content lower than 5%.
(2) Degreasing: adding absolute ethyl alcohol which accounts for 5 times of the mass of the dry powder of the sea horse powder into the sea horse powder after the step (1), uniformly mixing, then placing the sea horse powder mixed with the slurry into an ultrasonic extraction device, setting the ultrasonic frequency to be 35KHz, setting the temperature in the extraction device to be 45 ℃, centrifuging the solution after ultrasonic extraction by using a centrifugal machine, and then filtering to obtain the degreased sea horse powder.
(3) Primary extraction: drying the degreased hippocampus powder obtained in the step (2) at low temperature, adding NaOH solution with the concentration of 0.05-0.08mol/L into the dried hippocampus powder, fully stirring, and adding NaOH solution according to the proportion that the hippocampus powder/NaOH solution is equal to 1: 20g/mol, and placing the stirred and mixed solution into an ultrasonic reactor for ultrasonic catalysis for 3-5min to obtain a primary extracting solution of the hippocampus protein.
(4) Separating out and extracting: and (3) adjusting the pH value of the crude hippocampal protein extract obtained in the step (3) to be neutral, carrying out low-temperature concentration on the crude protein extract, stopping concentration when the solution is concentrated to the beginning of crystallization, adding absolute ethyl alcohol with the same amount of an organic solvent into the concentrated solution, stirring uniformly, slowly adding NaCI until NaCI is not dissolved, adding absolute ethyl alcohol with 1/5 organic solvent, and dissolving excessive NaCI to obtain a mixed solution of the hippocampal protein.
(5) And (4) passing the mixed solution of the hippocampal protein in the step (4) through a filter, removing the mixed solution, and drying the obtained hippocampal protein powder.
Example 6
A crude extraction process of hippocampal protein comprises the following steps:
(1) pretreatment of the hippocampus: cleaning the sea horse, sterilizing, drying, crushing and grinding to obtain sea horse powder with water content lower than 5%.
(2) Degreasing: adding absolute ethyl alcohol which accounts for 5 times of the mass of the dry powder of the sea horse powder into the sea horse powder after the step (1), uniformly mixing, then placing the sea horse powder mixed with the slurry into an ultrasonic extraction device, setting the ultrasonic frequency to be 35KHz, setting the temperature in the extraction device to be 45 ℃, centrifuging the solution after ultrasonic extraction by using a centrifugal machine, and then filtering to obtain the degreased sea horse powder.
(3) Primary extraction: drying the degreased hippocampus powder obtained in the step (2) at low temperature, adding NaOH solution with the concentration of 0.05-0.08mol/L into the dried hippocampus powder, fully stirring, and adding NaOH solution according to the proportion that the hippocampus powder/NaOH solution is equal to 1: 10g/mol, and placing the stirred and mixed solution into an ultrasonic reactor for ultrasonic catalysis for 3-5min to obtain a primary extracting solution of the hippocampus protein.
(4) Separating out and extracting: and (3) adjusting the pH value of the crude hippocampal protein extract obtained in the step (3) to be neutral, carrying out low-temperature concentration on the crude protein extract, stopping concentration when the solution is concentrated to the beginning of crystallization, adding absolute ethyl alcohol with the same amount of an organic solvent into the concentrated solution, stirring uniformly, slowly adding NaCI until NaCI is not dissolved, adding absolute ethyl alcohol with 1/5 organic solvent, and dissolving excessive NaCI to obtain a mixed solution of the hippocampal protein.
(5) And (4) passing the mixed solution of the hippocampal protein in the step (4) through a filter, removing the mixed solution, and drying the obtained hippocampal protein powder.
Performance evaluation:
1. content determination of extracted protein: kjeldahl method.
2. And (3) fat content determination: soxhlet extraction.
3. And (3) ash content determination: refer to GB5009.4-2010 burning constant repeats.
4. And (3) moisture determination: refer to GB5009.3-2016 for direct drying.
The proteins extracted in examples 1-6 were subjected to potentiostatic testing, the results of which are shown in Table 1:
of course, the above description is not limited to the above examples, and the undescribed technical features of the present invention can be implemented by or using the prior art, and will not be described herein again; the above embodiments are merely for illustrating the technical solutions of the present invention and not for limiting the present invention, and the present invention has been described in detail with reference to the preferred embodiments, and those skilled in the art should understand that changes, modifications, additions or substitutions which are made by those skilled in the art within the spirit of the present invention are also within the scope of the claims of the present invention.
Claims (4)
1. A crude extraction process of hippocampal protein is characterized by comprising the following steps: the method comprises the following steps: (1) pretreatment of the hippocampus: cleaning impurities of the sea horse, sterilizing, drying, crushing and grinding to obtain sea horse powder, (2) degreasing: adding absolute ethyl alcohol which accounts for 3-5 times of the mass of the dry powder of the sea horse powder into the sea horse powder after the step (1), uniformly mixing, then placing the sea horse powder mixed with the slurry into an ultrasonic extraction device, wherein the ultrasonic frequency is 25KHz-35KHz, the temperature in the extraction device is set to be 40-45 ℃, centrifuging the solution after the ultrasonic extraction by using a centrifugal machine, and then filtering to obtain degreased sea horse powder, (3) preliminarily extracting: drying the degreased hippocampus powder obtained in the step (2) at low temperature, adding NaOH solution with the concentration of 0.05-0.08mol/L into the dried hippocampus powder, fully stirring, and adding NaOH solution according to the proportion that the hippocampus powder/NaOH solution is equal to 1: 10-20g/mol, placing the stirred and mixed solution into an ultrasonic reactor for ultrasonic catalysis for 3-5min to obtain a primary extracting solution of the hippocampal protein, and (4) separating out and extracting: adjusting the pH value of the crude hippocampal protein extract obtained in the step (3) to be neutral, carrying out low-temperature concentration on the crude protein extract, stopping concentration when the solution is concentrated to the beginning of crystallization, adding absolute ethyl alcohol with the same amount of a body machine into the concentrated solution, stirring uniformly, slowly adding NaCI until NaCI is not dissolved, adding absolute ethyl alcohol with 1/5 body machines until excessive NaCI is dissolved, obtaining mixed solution of the hippocampal protein, (5) passing the mixed solution of the hippocampal protein obtained in the step (4) through a filter, removing the mixed solution, and then drying the obtained hippocampal protein powder.
2. The crude extraction process of hippocampal proteins of claim 1, wherein: the water content of the dry hippocampus powder in the step (1) is lower than 5%.
3. The crude extraction process of hippocampal proteins of claim 1, wherein: the ultrasonic power of the ultrasonic extraction device is 25KHz-35 KHz.
4. The crude extraction process of hippocampal proteins of claim 1, wherein: the rotating speed of the centrifuge is 3000-.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103923201A (en) * | 2014-04-01 | 2014-07-16 | 宁波大学 | Preparation method of hippocampus active glycoprotein |
| CN104152517A (en) * | 2013-05-15 | 2014-11-19 | 上海海洋大学 | Method for extracting short peptides from sea horses |
| CN109371090A (en) * | 2018-12-25 | 2019-02-22 | 浙江鼎益生物科技有限公司 | A kind of process for extracting bread worm protein |
| CN208790164U (en) * | 2018-08-28 | 2019-04-26 | 深圳市科芙海洋科技有限公司 | A kind of hippocampal protein biological reagent extraction element |
| KR20210038157A (en) * | 2019-09-30 | 2021-04-07 | 제주대학교 산학협력단 | Composition for preventing or treating inflammatory disease comprising Hippocampus abdominalis extract or fractions thereof |
-
2022
- 2022-04-01 CN CN202210337535.2A patent/CN114478687A/en active Pending
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|---|---|---|---|---|
| CN104152517A (en) * | 2013-05-15 | 2014-11-19 | 上海海洋大学 | Method for extracting short peptides from sea horses |
| CN103923201A (en) * | 2014-04-01 | 2014-07-16 | 宁波大学 | Preparation method of hippocampus active glycoprotein |
| CN208790164U (en) * | 2018-08-28 | 2019-04-26 | 深圳市科芙海洋科技有限公司 | A kind of hippocampal protein biological reagent extraction element |
| CN109371090A (en) * | 2018-12-25 | 2019-02-22 | 浙江鼎益生物科技有限公司 | A kind of process for extracting bread worm protein |
| KR20210038157A (en) * | 2019-09-30 | 2021-04-07 | 제주대학교 산학협력단 | Composition for preventing or treating inflammatory disease comprising Hippocampus abdominalis extract or fractions thereof |
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| Title |
|---|
| YUTING SU: "Study on the extraction and purifi cation of glycoprotein from the yellow seahorse, Hippocampus kuda Bleeker", 《FOOD SCI NUTR》, vol. 3, no. 4, pages 302 - 312 * |
| 徐永健: "海马糖蛋白结构的初步鉴定及其 抗氧化特性分析", 《中国海洋药物》, vol. 36, no. 6, pages 1 * |
| 胡佳瑶: "海马总蛋白提取及其酶解条件优化", 《生物技术进展》, vol. 7, no. 4, pages 310 - 314 * |
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