CN103193861A - Method for extracting protein from waste liquid generated from production of sodium heparin - Google Patents
Method for extracting protein from waste liquid generated from production of sodium heparin Download PDFInfo
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- CN103193861A CN103193861A CN2013101112527A CN201310111252A CN103193861A CN 103193861 A CN103193861 A CN 103193861A CN 2013101112527 A CN2013101112527 A CN 2013101112527A CN 201310111252 A CN201310111252 A CN 201310111252A CN 103193861 A CN103193861 A CN 103193861A
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Abstract
The invention provides a method for extracting protein from a waste liquid generated from production of sodium heparin. According to the method, crude protein and high-concentration protein are obtained through the technological process of PH value adjustment, settling, centrifugal separation and concentration. The method has the beneficial effects that (1) the waste liquid is recovered for extracting protein amino acids and the waste is turned into a high-protein animal feed which can be used for development of the breeding industry, so that great economic benefit is obtained; (2) compliance of recovered waste liquid with relevant standards protein can be met as protein in the recovered waste liquid is extracted, so that the discharging of the waste liquid does not result in environmental pollution, and the method has social benefit and is beneficial to the living environment of people.
Description
Technical field
The present invention relates to a kind of from the waste liquid of producing heparin sodium method for extracting proteins.
Background technology
Heparin sodium is a kind of material that extracts from pig intestinal mucosa, after mucous membrane of small intestine becomes liquid by enzymolysis, in liquid, add and resin dedicated absorption of heparin sodium namely get heparin sodium, absorption back waste liquid directly discharges, or carry out biochemical treatment, be difficult to up to standardly owing to contain a large amount of Argine Monohydrochloride biochemical treatments in the waste liquid, be easy to cause environmental pollution, also caused resource to waste greatly simultaneously.
Summary of the invention
The present invention proposes a kind of from the waste liquid of producing heparin sodium method for extracting proteins, solved deficiency of the prior art, reclaim by waste liquid, can extract protein, make refuse become the high protein animal-feed, neither cause environmental pollution, also can be used for developing aquaculture, both had an economic benefit, social benefit was also arranged.
Technical scheme of the present invention be achieved in that a kind of from the waste liquid of producing heparin sodium method for extracting proteins, it may further comprise the steps:
(1) pH value with waste liquid is adjusted to 8-10;
(2) add precipitation agent in waste liquid, dissolving is under agitation reacted 30-60min fully then under stirring condition, close the stirring standing demix then;
(3) waste liquid behind the standing demix is carried out centrifugation, namely get crude protein behind the isolated solid drying, collect isolated supernatant liquid, described crude protein is high protein feed, can be directly used in the aquaculture;
(4) isolated supernatant liquid is pumped in the nanofiltration membrane of 150-300 molecular weight and concentrates, when being concentrated into the 1/50-1/40 of former isolated supernatant liquid volume, collect concentrated solution, in concentrated solution, add alcohol, place precipitation 24-28h when making its ethanol concn reach 35-45%, supernatant liquid is removed in siphon, and lower sediment is collected the protein that is drying to obtain high-content, can be used in the aquaculture equally.
Further, the used reagent of waste liquid pH value adjustment is the sodium hydroxide of 30-40% in the described step (1).
Further, the add-on of precipitation agent is the 0.1-0.3% of waste liquid quality in the described step (2).
Further, used precipitation agent is calcium chloride or aluminum chloride in the described step (2).
The rotating speed of used whizzer is 4000-5000r/min when further, carrying out centrifugation in the described step (3).
Further, adding alcohol in the described step (4) is the alcohol of 90-95%.
Compared with prior art, the invention has the beneficial effects as follows:
(1) reclaims by waste liquid, extract Argine Monohydrochloride, make refuse become the high protein animal-feed, can be used for developing aquaculture, very large economy benefit is arranged;
(2) reclaim by waste liquid, the proteins extraction in the former waste liquid is come out, it is carried out biochemical treatment no longer be difficult to up to standardly, in discharging, can not cause environmental pollution like this, have social benefit, favourable to people's living environment.
Embodiment
Below the technical scheme among the present invention is clearly and completely described, obviously, described embodiment only is several embodiment wherein of the present invention, rather than whole embodiment.Based on the embodiment among the present invention, those of ordinary skills belong to the scope of protection of the invention not making the every other embodiment that obtains under the creative work prerequisite.
Embodiment 1:
A kind of from the waste liquid of producing heparin sodium method for extracting proteins, it may further comprise the steps:
(1) pH value of 10000Kg waste liquid is adjusted to 8 with 30% sodium hydroxide;
(2) add the calcium chloride of waste liquid quality 0.1% in the waste liquid, namely the quality of used calcium chloride is 10Kg, and under agitation dissolving is reacted 30min fully then under stirring condition then, closes the stirring standing demix then;
(3) waste liquid behind the standing demix is carried out centrifugation, the centrifugal rotational speed of whizzer is 4000r/min, namely gets 35.2% crude protein 352.8Kg behind the isolated solid drying, collects isolated supernatant liquid;
(4) isolated supernatant liquid is pumped in the nanofiltration membrane of 150 molecular weight and concentrates, be concentrated into 1/40 o'clock of former isolated supernatant liquid volume, collect concentrated solution, the alcohol of adding 90% in concentrated solution, make its ethanol concn reach at 45% o'clock and place precipitation 26h, supernatant liquid is removed in siphon, collects lower sediment, with the protein 151.4Kg that namely gets 53.5% after the lower sediment drying.
Embodiment 2:
A kind of from the waste liquid of producing heparin sodium method for extracting proteins, it may further comprise the steps:
(1) pH value of 10000Kg waste liquid is adjusted to 8.5 with 35% sodium hydroxide;
(2) add the calcium chloride of waste liquid quality 0.2% in the waste liquid, namely the quality of used calcium chloride is 10Kg, and under agitation dissolving is reacted 40min fully then under stirring condition then, closes the stirring standing demix then;
(3) waste liquid behind the standing demix is carried out centrifugation, the centrifugal rotational speed of whizzer is 4500r/min, namely gets 37.8% crude protein 349.5Kg behind the isolated solid drying, collects isolated supernatant liquid;
(4) isolated supernatant liquid is pumped in the nanofiltration membrane of 200 molecular weight and concentrates, be concentrated into 1/45 o'clock of former isolated supernatant liquid volume, collect concentrated solution, the alcohol of adding 95% in concentrated solution, make its ethanol concn reach at 40% o'clock and place precipitation 24h, supernatant liquid is removed in siphon, collects lower sediment, with the protein 150.2Kg that namely gets 56.5% after the lower sediment drying.
Embodiment 3:
A kind of from the waste liquid of producing heparin sodium method for extracting proteins, it may further comprise the steps:
(1) pH value of 10000Kg waste liquid is adjusted to 9.5 with 40% sodium hydroxide;
(2) add the aluminum chloride of waste liquid quality 0.3% in the waste liquid, dissolving is under agitation reacted 60min fully then under stirring condition, close the stirring standing demix then;
(3) waste liquid behind the standing demix is carried out centrifugation, the centrifugal rotational speed of whizzer is 5000r/min, namely gets 36.8% crude protein 353.6Kg behind the isolated solid drying, collects isolated supernatant liquid;
(4) isolated supernatant liquid is pumped in the nanofiltration membrane of 300 molecular weight and concentrates, be concentrated into 1/50 o'clock of former isolated supernatant liquid volume, collect concentrated solution, the alcohol of adding 90% in concentrated solution, make its ethanol concn reach at 35% o'clock and place precipitation 28h, supernatant liquid is removed in siphon, collects lower sediment, with the protein 151.8Kg that namely gets 55.8% after the lower sediment drying.
The above only is preferred embodiment of the present invention, and is in order to limit the present invention, within the spirit and principles in the present invention not all, any modification of doing, is equal to replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (6)
1. method for extracting proteins from the waste liquid of producing heparin sodium, it is characterized in that: it may further comprise the steps:
(1) pH value with waste liquid is adjusted to 8-10;
(2) add precipitation agent in waste liquid, dissolving is under agitation reacted 30-60min fully then under stirring condition, close the stirring standing demix then;
(3) waste liquid behind the standing demix is carried out centrifugation, namely get crude protein behind the isolated solid drying, collect isolated supernatant liquid;
(4) isolated supernatant liquid is pumped in the nanofiltration membrane of 150-300 molecular weight and concentrates, when being concentrated into the 1/50-1/40 of former isolated supernatant liquid volume, collect concentrated solution, in concentrated solution, add alcohol, place precipitation 24-28h when making its ethanol concn reach 35-45%, supernatant liquid is removed in siphon, lower sediment is collected the protein that is drying to obtain high-content.
2. according to a kind of described in the claim 1 method for extracting proteins from the waste liquid of producing heparin sodium, it is characterized in that: to adjust used reagent be the sodium hydroxide of 30-40% to the waste liquid pH value in the described step (1).
3. according to a kind of described in the claim 1 method for extracting proteins from the waste liquid of producing heparin sodium, it is characterized in that: the add-on of precipitation agent is the 0.1-0.3% of waste liquid quality in the described step (2).
4. according to a kind of described in the claim 3 method for extracting proteins from the waste liquid of producing heparin sodium, it is characterized in that: used precipitation agent is calcium chloride or aluminum chloride in the described step (2).
5. according to a kind of described in the claim 1 method for extracting proteins from the waste liquid of producing heparin sodium, it is characterized in that: the rotating speed of used whizzer is 4000-5000r/min when carrying out centrifugation in the described step (3).
6. according to a kind of described in the claim 1 method for extracting proteins from the waste liquid of producing heparin sodium, it is characterized in that: adding alcohol in the described step (4) is the alcohol of 90-95%.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310111252.7A CN103193861B (en) | 2013-04-01 | 2013-04-01 | Method for extracting protein from waste liquid generated from production of sodium heparin |
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| CN201310111252.7A CN103193861B (en) | 2013-04-01 | 2013-04-01 | Method for extracting protein from waste liquid generated from production of sodium heparin |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103936888A (en) * | 2014-02-21 | 2014-07-23 | 黄�良 | Method used for waste liquor recovery, and heparin sodium and feed protein extraction after extraction of heparin sodium via enzymolysis |
| CN104262509A (en) * | 2014-09-25 | 2015-01-07 | 韩敬冰 | Production process for secondary extraction of heparin sodium from enzymolysis adsorption waste liquid |
| CN106538841A (en) * | 2016-10-26 | 2017-03-29 | 上海新农饲料股份有限公司 | A kind of dried porcine saluble product and its production method |
| CN110217911A (en) * | 2019-06-25 | 2019-09-10 | 广元市海鹏生物科技有限公司 | A kind of heparin sodium wastewater treatment process of high efficiente callback protein |
| CN113831389A (en) * | 2021-10-26 | 2021-12-24 | 重庆望业生物制药有限公司 | Method for preparing compound protein amino acid product by using waste liquid from animal viscera extraction |
| CN116235897A (en) * | 2023-03-14 | 2023-06-09 | 大连海洋大学 | Novel low-energy-consumption and environment-friendly fish meal production process and system |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102295711A (en) * | 2011-08-23 | 2011-12-28 | 湖北宝迪农业科技有限公司 | Technology for extracting heparin sodium from pig lungs with polypeptide protein powder as combined product |
| CN102731683A (en) * | 2012-07-17 | 2012-10-17 | 湖北亿诺瑞生物制药有限公司 | Method of separating natural low molecular heparin from heparin waste liquor |
| CN102876763A (en) * | 2012-09-27 | 2013-01-16 | 四川茂森生物科技有限公司 | Method for comprehensively utilizing waste residues and liquid waste of animal biochemical products |
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2013
- 2013-04-01 CN CN201310111252.7A patent/CN103193861B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102295711A (en) * | 2011-08-23 | 2011-12-28 | 湖北宝迪农业科技有限公司 | Technology for extracting heparin sodium from pig lungs with polypeptide protein powder as combined product |
| CN102731683A (en) * | 2012-07-17 | 2012-10-17 | 湖北亿诺瑞生物制药有限公司 | Method of separating natural low molecular heparin from heparin waste liquor |
| CN102876763A (en) * | 2012-09-27 | 2013-01-16 | 四川茂森生物科技有限公司 | Method for comprehensively utilizing waste residues and liquid waste of animal biochemical products |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103936888A (en) * | 2014-02-21 | 2014-07-23 | 黄�良 | Method used for waste liquor recovery, and heparin sodium and feed protein extraction after extraction of heparin sodium via enzymolysis |
| CN104262509A (en) * | 2014-09-25 | 2015-01-07 | 韩敬冰 | Production process for secondary extraction of heparin sodium from enzymolysis adsorption waste liquid |
| CN106538841A (en) * | 2016-10-26 | 2017-03-29 | 上海新农饲料股份有限公司 | A kind of dried porcine saluble product and its production method |
| CN110217911A (en) * | 2019-06-25 | 2019-09-10 | 广元市海鹏生物科技有限公司 | A kind of heparin sodium wastewater treatment process of high efficiente callback protein |
| CN113831389A (en) * | 2021-10-26 | 2021-12-24 | 重庆望业生物制药有限公司 | Method for preparing compound protein amino acid product by using waste liquid from animal viscera extraction |
| CN116235897A (en) * | 2023-03-14 | 2023-06-09 | 大连海洋大学 | Novel low-energy-consumption and environment-friendly fish meal production process and system |
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| Publication number | Publication date |
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| CN103193861B (en) | 2015-06-24 |
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