CN114478621B - Process for extracting enzymatic soybean phospholipids - Google Patents
Process for extracting enzymatic soybean phospholipids Download PDFInfo
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- CN114478621B CN114478621B CN202210085101.8A CN202210085101A CN114478621B CN 114478621 B CN114478621 B CN 114478621B CN 202210085101 A CN202210085101 A CN 202210085101A CN 114478621 B CN114478621 B CN 114478621B
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- 239000008347 soybean phospholipid Substances 0.000 title claims abstract description 42
- 238000000034 method Methods 0.000 title claims abstract description 28
- 230000002255 enzymatic effect Effects 0.000 title description 3
- 235000010469 Glycine max Nutrition 0.000 claims abstract description 40
- 244000068988 Glycine max Species 0.000 claims abstract description 40
- 238000001471 micro-filtration Methods 0.000 claims abstract description 28
- 239000012528 membrane Substances 0.000 claims abstract description 27
- JLPULHDHAOZNQI-ZTIMHPMXSA-N 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCC\C=C/C\C=C/CCCCC JLPULHDHAOZNQI-ZTIMHPMXSA-N 0.000 claims abstract description 25
- 238000001035 drying Methods 0.000 claims abstract description 23
- 239000006228 supernatant Substances 0.000 claims abstract description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 17
- 238000001914 filtration Methods 0.000 claims abstract description 15
- 239000007788 liquid Substances 0.000 claims abstract description 14
- 239000000839 emulsion Substances 0.000 claims abstract description 12
- 230000007071 enzymatic hydrolysis Effects 0.000 claims abstract description 12
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims abstract description 12
- 239000011259 mixed solution Substances 0.000 claims abstract description 11
- 238000010438 heat treatment Methods 0.000 claims abstract description 9
- 108091005658 Basic proteases Proteins 0.000 claims abstract description 8
- 239000003921 oil Substances 0.000 claims abstract description 8
- 108090000604 Hydrolases Proteins 0.000 claims abstract description 6
- 102000004157 Hydrolases Human genes 0.000 claims abstract description 6
- 230000036571 hydration Effects 0.000 claims abstract description 6
- 238000006703 hydration reaction Methods 0.000 claims abstract description 6
- 239000000413 hydrolysate Substances 0.000 claims abstract description 6
- 238000002156 mixing Methods 0.000 claims abstract description 5
- 238000004140 cleaning Methods 0.000 claims abstract description 4
- 238000012216 screening Methods 0.000 claims abstract description 4
- 238000007599 discharging Methods 0.000 claims description 44
- 238000005192 partition Methods 0.000 claims description 30
- 230000000903 blocking effect Effects 0.000 claims description 22
- 238000003860 storage Methods 0.000 claims description 6
- 239000000843 powder Substances 0.000 abstract description 17
- 239000000463 material Substances 0.000 description 9
- 235000019198 oils Nutrition 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 230000003028 elevating effect Effects 0.000 description 5
- 230000033001 locomotion Effects 0.000 description 5
- 238000000605 extraction Methods 0.000 description 4
- 229940083466 soybean lecithin Drugs 0.000 description 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 238000005054 agglomeration Methods 0.000 description 2
- 230000002776 aggregation Effects 0.000 description 2
- 239000006185 dispersion Substances 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- PORPENFLTBBHSG-MGBGTMOVSA-N 1,2-dihexadecanoyl-sn-glycerol-3-phosphate Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP(O)(O)=O)OC(=O)CCCCCCCCCCCCCCC PORPENFLTBBHSG-MGBGTMOVSA-N 0.000 description 1
- TZCPCKNHXULUIY-RGULYWFUSA-N 1,2-distearoyl-sn-glycero-3-phosphoserine Chemical compound CCCCCCCCCCCCCCCCCC(=O)OC[C@H](COP(O)(=O)OC[C@H](N)C(O)=O)OC(=O)CCCCCCCCCCCCCCCCC TZCPCKNHXULUIY-RGULYWFUSA-N 0.000 description 1
- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 description 1
- CFWRDBDJAOHXSH-SECBINFHSA-N 2-azaniumylethyl [(2r)-2,3-diacetyloxypropyl] phosphate Chemical compound CC(=O)OC[C@@H](OC(C)=O)COP(O)(=O)OCCN CFWRDBDJAOHXSH-SECBINFHSA-N 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- ZWZWYGMENQVNFU-UHFFFAOYSA-N Glycerophosphorylserin Natural products OC(=O)C(N)COP(O)(=O)OCC(O)CO ZWZWYGMENQVNFU-UHFFFAOYSA-N 0.000 description 1
- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- OEYIOHPDSNJKLS-UHFFFAOYSA-N choline Chemical compound C[N+](C)(C)CCO OEYIOHPDSNJKLS-UHFFFAOYSA-N 0.000 description 1
- 229960001231 choline Drugs 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 238000004945 emulsification Methods 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 229960000367 inositol Drugs 0.000 description 1
- -1 inositol phospholipids Chemical class 0.000 description 1
- 229940067606 lecithin Drugs 0.000 description 1
- 239000000787 lecithin Substances 0.000 description 1
- 235000010445 lecithin Nutrition 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005374 membrane filtration Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000037257 muscle growth Effects 0.000 description 1
- 230000008271 nervous system development Effects 0.000 description 1
- 239000012454 non-polar solvent Substances 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 150000003904 phospholipids Chemical class 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 235000012424 soybean oil Nutrition 0.000 description 1
- 239000003549 soybean oil Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000009736 wetting Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
- C07F9/02—Phosphorus compounds
- C07F9/06—Phosphorus compounds without P—C bonds
- C07F9/08—Esters of oxyacids of phosphorus
- C07F9/09—Esters of phosphoric acids
- C07F9/10—Phosphatides, e.g. lecithin
- C07F9/103—Extraction or purification by physical or chemical treatment of natural phosphatides; Preparation of compositions containing phosphatides of unknown structure
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Beans For Foods Or Fodder (AREA)
Abstract
The invention discloses a process for extracting enzymatic hydrolysis soybean phospholipids, which comprises the following steps: A. screening full soybeans, cleaning the soybeans, and standing and drying; B. heating and crushing the dried soybeans, mixing the crushed soybeans with water to obtain a mixed solution, adding alkaline protease into the mixed solution for enzymolysis, and centrifuging after enzymolysis to obtain free oil, emulsion, hydrolysate and residues; C. adding carboxylic acid hydrolase into the emulsion obtained in the step B for enzymolysis, and then adding water for hydration to obtain supernatant; D. drying and filtering the supernatant liquid simultaneously by using a microfiltration membrane filter to obtain soybean phospholipid; the process can make the soybean powder fully react during enzymolysis, and improve the utilization rate of components in the soybean.
Description
Technical Field
The invention relates to the technical field of soybean phospholipid extraction, in particular to a process for extracting enzymatic hydrolysis soybean phospholipid.
Background
The soybean phospholipids are extracted from the oil residue of soybean oil production, and are esters composed of glycerol, fatty acid, choline or cholic amine, and can be dissolved in oil and nonpolar solvent. Soybean phospholipids are complex in composition and mainly contain lecithin (about 34.2%), cephalin (about 19.7%), inositol phospholipids (about 16.0%), phosphatidylserine (about 15.8%), phosphatidic acid (about 3.6%) and other phospholipids (about 10.7%). Is a light yellow to brown viscous liquid or a white to light brown solid powder.
The soybean lecithin not only has strong emulsification, wetting and dispersion effects, but also plays an important role in promoting in-vivo fat metabolism, muscle growth, nervous system development, in-vivo oxidation injury resistance and the like.
At present, in the process of extracting soybeans by adopting an enzymolysis method, the problem of insufficient reaction between soybean powder and enzyme often occurs, so that the components in the soybeans are wasted.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides a process for extracting enzymatic hydrolysis soybean lecithin, which can lead soybean powder to fully react during enzymatic hydrolysis and improve the utilization rate of components in the soybean.
A process for extracting enzymatic hydrolysis soybean phospholipids comprises the following steps:
A. screening full soybeans, cleaning the soybeans, and standing and drying;
B. heating and crushing the dried soybeans, mixing the crushed soybeans with water to obtain a mixed solution, adding alkaline protease into the mixed solution for enzymolysis, and centrifuging after enzymolysis to obtain free oil, emulsion, hydrolysate and residues;
C. adding carboxylic acid hydrolase into the emulsion obtained in the step B for enzymolysis, and then adding water for hydration to obtain supernatant;
D. drying and filtering the supernatant liquid simultaneously by using a microfiltration membrane filter to obtain the soybean phospholipid.
Preferably, the microfiltration membrane filter comprises a box body with an open top and a plurality of drying and filtering units, wherein an upper partition plate, a middle partition plate and a lower partition plate are arranged in the box body, the upper partition plate, the middle partition plate and the lower partition plate divide the inner cavity of the box body into a top cavity, an upper cavity, a middle cavity and a lower cavity, a rotating shaft is rotatably arranged in the middle cavity, a second gear is sleeved on the rotating shaft, a motor is arranged in the middle cavity, a first gear is connected to an output shaft of the motor, and the first gear is meshed with the second gear;
a plurality of drying and filtering units are horizontally distributed, each drying and filtering unit comprises a sphere, a feeding pipe, a discharging pipe, a first lifting device, a second lifting device, an air inlet box, an air inlet pipe, an air inlet machine, a storage box and a plurality of air exhaust pipe groups,
the sphere is fixed on the rotating shaft, the rotating shaft penetrates through the sphere, a plurality of through holes are formed in the sphere, the micro-filtration membrane is covered on the inner wall of the sphere, the feeding pipe is fixed on the top end of the sphere, the discharging pipe is fixed on the bottom end of the sphere, the feeding valve is arranged on the feeding pipe, the discharging valve is arranged on the discharging pipe,
the rotating shaft is internally provided with a plurality of cavities, the cavities are in one-to-one correspondence with the spheres, one part of the cavities is positioned in the spheres, the other part of the cavities is positioned outside the spheres, a plurality of exhaust pipe groups are arranged on the rotating shaft and are horizontally distributed, the exhaust pipe groups are positioned in the spheres, at least one exhaust pipe is arranged in the exhaust pipe groups, the exhaust pipe is communicated with the cavities,
the air inlet box is fixed in the middle cavity through the supporting component, the rotating shaft rotates to penetrate through the left wall and the right wall of the air inlet box, the air inlet box is positioned outside the sphere, the side wall of the cavity is provided with an air inlet hole, the air inlet hole is positioned in the inner cavity of the air inlet box, the output end of the air inlet machine is connected with the air inlet pipe, the air inlet pipe is internally provided with a heating pipe, the air inlet pipe is communicated with the inner cavity of the air inlet box,
the upper partition plate is provided with a plurality of liquid discharge holes, the liquid discharge holes are in one-to-one correspondence with the feeding pipes, the first lifting device is arranged in the upper cavity, the output end of the first lifting device is connected with the feeding pipes, the top ends of the feeding pipes enter the liquid discharge holes, the bottom ends of the feeding pipes penetrate through the middle partition plate to enter the middle cavity, and the bottom ends of the feeding pipes can enter the feeding pipes;
the receiver all sets up in the lower intracavity with second elevating gear, and second elevating gear's output is connected with row material pipe, and row material pipe's bottom is located the receiver, and row material pipe's top passes down the baffle and gets into in the chamber, row material pipe top can get into the discharging intraductal.
Preferably, the feeding valve comprises an upper blocking block, an upper fixing plate, an upper spring and an upper connecting rod, wherein the upper fixing plate is fixed on the outer wall of the feeding pipe through the upper connecting rod, the upper blocking block movably penetrates through the right wall of the feeding pipe, the upper spring is connected between the right wall of the upper blocking block and the left wall of the upper fixing plate, the left wall of the upper blocking block is integrally inclined downwards in the direction from the right side to the left side, and the bottom end of the feeding pipe moves downwards to prop against the upper blocking block to move to the right side.
Preferably, the discharging valve comprises a lower plugging block, a lower fixing plate, a lower spring and a lower connecting rod, wherein the lower fixing plate is fixed on the outer wall of the discharging pipe through the lower connecting rod, the lower plugging block movably penetrates through the right wall of the discharging pipe, the lower spring is connected between the right wall of the lower plugging block and the left wall of the lower fixing plate, the left wall of the lower plugging block is integrally inclined downwards in the direction from left side to right side, and the top end of the discharging pipe moves upwards to prop against the lower plugging block to move to the right side.
Preferably, the middle cavity left wall is provided with drive arrangement, and drive arrangement's output is connected with the fixed plate, and drive arrangement can drive the fixed plate and control the motion, is provided with two mounting panels on the fixed plate, and two mounting panels distribute along the fore-and-aft direction, are provided with clamping unit on the mounting panel, and clamping unit on two mounting panels can carry out the centre gripping to leftmost discharging pipe.
Preferably, the clamping unit comprises an air cylinder and clamping plates, the air cylinder is fixed on the mounting plate, the output end of the air cylinder is connected with the clamping plates, and the two clamping plates can clamp the discharging pipe.
Preferably, the exhaust pipe group comprises a plurality of exhaust pipes, and the exhaust pipes in the exhaust pipe group are distributed around the circumference of the rotating shaft.
Preferably, the plurality of exhaust pipes in the exhaust pipe group are uniformly distributed around the circumference of the rotating shaft.
The beneficial effects of the invention are as follows: in the technical scheme, firstly, the soybeans with full grains are screened, cleaned, placed and dried; heating and crushing the dried soybeans, mixing the crushed soybeans with water to obtain a mixed solution, adding alkaline protease into the mixed solution for enzymolysis, and centrifuging after enzymolysis to obtain free oil, emulsion, hydrolysate and residues; adding carboxylic acid hydrolase into the obtained emulsion for enzymolysis, and then adding water for hydration to obtain supernatant; finally, drying and filtering the supernatant fluid simultaneously by using a microfiltration membrane filter to obtain soybean phospholipid; according to the technical scheme, the soybean powder is dried in the process of grinding the soybean, so that the formed soybean powder is prevented from caking, the soybean powder can be fully mixed with water when the soybean powder is mixed with the water, and then alkaline protease is added for enzymolysis, so that the soybean powder can be fully subjected to enzymolysis, the soybean powder can be fully reacted during enzymolysis, and the utilization rate of components in the soybean is improved.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. Like elements or portions are generally identified by like reference numerals throughout the several figures. In the drawings, elements or portions thereof are not necessarily drawn to scale.
FIG. 1 is a front cross-sectional view of the present invention;
FIG. 2 is an enlarged schematic view of the structure of the position A in FIG. 1;
fig. 3 is an enlarged schematic view of the B position in fig. 1.
In the drawing, a 1-box body, a 2-upper partition plate, a 3-middle partition plate, a 4-lower partition plate, a 5-rotating shaft, a 6-motor, a 7-first gear, an 8-second gear, a 9-sphere, a 10-feeding pipe, an 11-discharging pipe, a 12-feeding pipe, a 13-discharging pipe, a 14-first lifting device, a 15-second lifting device, a 16-air inlet box, a 17-air inlet pipe, a 18-air inlet machine, a 19-exhaust pipe, a 20-driving device, a 21-fixing plate, a 22-mounting plate, a 23-clamping plate, a 24-cavity, a 25-storage box, a 26-upper sealing block, a 27-upper fixing plate, a 28-upper spring, a 29-upper connecting rod, a 30-lower sealing block, a 31-lower fixing plate, a 32-lower spring and a 33-lower connecting rod.
Detailed Description
Embodiments of the technical scheme of the present invention will be described in detail below with reference to the accompanying drawings. The following examples are only for more clearly illustrating the technical aspects of the present invention, and thus are merely examples, and are not intended to limit the scope of the present invention.
It is noted that unless otherwise indicated, technical or scientific terms used herein should be given the ordinary meaning as understood by one of ordinary skill in the art to which this invention pertains.
Example 1
As shown in fig. 1-3, the present embodiment provides a process for extracting enzymatic soybean phospholipids, which comprises the following steps:
A. screening full soybeans, cleaning the soybeans, and standing and drying;
B. heating and crushing the dried soybeans, mixing the crushed soybeans with water to obtain a mixed solution, adding alkaline protease into the mixed solution for enzymolysis, and centrifuging after enzymolysis to obtain free oil, emulsion, hydrolysate and residues;
C. adding carboxylic acid hydrolase into the emulsion obtained in the step B for enzymolysis, and then adding water for hydration to obtain supernatant;
D. drying and filtering the supernatant liquid simultaneously by using a microfiltration membrane filter to obtain the soybean phospholipid.
In the traditional process for extracting soybean lecithin by enzymolysis, soybean is crushed into powder, then water is added to mix to obtain a mixed solution, alkaline protease is added to carry out enzymolysis to separate free oil, emulsion, hydrolysate and residues, then carboxylic acid hydrolase is used for enzymolysis of the emulsion, supernatant is obtained after hydration, and microfiltration membrane filtration is carried out on the supernatant to obtain soybean lecithin.
In this embodiment, the soybean powder is dried in the process of pulverizing the soybean, so that the formed soybean powder is not agglomerated, the soybean powder can be fully mixed with water when being mixed with the water, and then alkaline protease is added for enzymolysis, so that the soybean powder can be fully subjected to enzymolysis.
The microfiltration membrane filter in the embodiment comprises a box body 1 with an open top and a plurality of drying and filtering units, wherein an upper partition plate 2, a middle partition plate 3 and a lower partition plate 4 are arranged in the box body 1, the upper partition plate 2, the middle partition plate 3 and the lower partition plate 4 divide the inner cavity of the box body into a top cavity, an upper cavity, a middle cavity and a lower cavity, a rotating shaft 5 is rotatably arranged in the middle cavity, a second gear 8 is sleeved on the rotating shaft 5, a motor 6 is arranged in the middle cavity, a first gear 7 is connected to an output shaft of the motor 6, and the first gear 7 is meshed with the second gear 8;
a plurality of drying and filtering units are horizontally distributed, each drying and filtering unit comprises a sphere 9, a feed pipe 10, a discharge pipe 11, a feed pipe 12, a discharge pipe 13, a first lifting device 14, a second lifting device 15, an air inlet box 16, an air inlet pipe 17, an air inlet fan 18, a storage box 25 and a plurality of air exhaust pipe groups,
the sphere 9 is fixed on the rotating shaft 5, the rotating shaft 5 passes through the sphere 9, a plurality of through holes are formed in the sphere 9, the micro-filtration membrane is covered on the inner wall of the sphere 9, the feeding pipe 10 is fixed at the top end of the sphere 9, the discharging pipe 11 is fixed at the bottom end of the sphere 9, the feeding pipe 10 is provided with a feeding valve, the discharging pipe 11 is provided with a discharging valve,
the rotating shaft 5 is internally provided with a plurality of cavities 24, the cavities 24 are in one-to-one correspondence with the spheres 9, one part of the cavities 24 is positioned in the spheres 9, the other part of the cavities 24 is positioned outside the spheres 9, a plurality of exhaust pipe groups are arranged on the rotating shaft 5 and are horizontally distributed, the exhaust pipe groups are positioned in the spheres 9, at least one exhaust pipe 19 is arranged in the exhaust pipe groups, the exhaust pipe 19 is communicated with the cavities 24,
the air inlet box 16 is fixed in the middle cavity through the supporting component, the rotating shaft 5 rotates to penetrate through the left wall and the right wall of the air inlet box 16, the air inlet box 16 is positioned outside the sphere 9, the side wall of the cavity 24 is provided with an air inlet hole, the air inlet hole is positioned in the inner cavity of the air inlet box 16, the output end of the air inlet machine 18 is connected with the air inlet pipe 17, the air inlet pipe 17 is internally provided with a heating pipe, the air inlet pipe 17 is communicated with the inner cavity of the air inlet box 16,
the upper partition plate 2 is provided with a plurality of liquid discharge holes, the liquid discharge holes are in one-to-one correspondence with the feeding pipes 12, the first lifting device 14 is arranged in the upper cavity, the output end of the first lifting device 14 is connected with the feeding pipes 12, the top ends of the feeding pipes 12 enter the liquid discharge holes, the bottom ends of the feeding pipes 12 penetrate through the middle partition plate 3 to enter the middle cavity, and the bottom ends of the feeding pipes 12 can enter the feeding pipes 10;
the receiver 25 and the second elevating gear 15 all set up in the lower chamber, and the output and the row material pipe 13 of second elevating gear 15 are connected, and the bottom of row material pipe 13 is located receiver 25, and the top of row material pipe 13 passes lower baffle 4 and gets into in the chamber, and row material pipe 13 top can get into in the discharging pipe 11.
In the process of extracting soybean phospholipids, the last step adopts a microfiltration membrane for filtration, and the soybean phospholipids are separated from the supernatant, in the step, the current working mode is that the microfiltration membrane is laid on a filter plate, then the supernatant is poured above the microfiltration membrane, so that water passes through the microfiltration membrane, and the soybean phospholipids are positioned on the microfiltration membrane, thereby realizing the separation of the soybean phospholipids. 2. When soybean phospholipid accumulated on the microfiltration membrane, because moisture can not be dried rapidly, soybean phospholipid can be agglomerated at the moment, the blockage of the microfiltration membrane is more serious after agglomeration, and soybean phospholipid is crushed and dried independently after agglomeration, so that the agglomerated soybean phospholipid is crushed, and the soybean phospholipid is ensured to be in a powdery state, so that the extraction efficiency of the traditional mode in the soybean phospholipid extraction process is very low.
In this embodiment, be provided with baffle 2, middle partition plate 3 and baffle 4 down in the box 1, go up baffle 2, middle partition plate 3 and baffle 4 divide into top chamber, upper chamber, well chamber and lower chamber with the inner chamber of box down, and motor 6 can drive pivot 5 rotation, and motor 6 adopts motor 6 that can idle running when not working.
The specific process principle is as follows, in the initial state, the bottom end of the feeding pipe 12 enters the feeding pipe 10, the top end of the discharging pipe 13 enters the discharging pipe 11, the feeding valve is in an open state, the discharging valve is in a closed state, supernatant is added into the top cavity at the moment, the supernatant enters the sphere 9 after passing through the feeding pipe 12 and the feeding pipe 10, then the feeding valve is closed, the first lifting device 14 is started to drive the feeding pipe 12 to move upwards, the feeding pipe 12 is separated from the feeding pipe 10, the second lifting device 15 is started to drive the discharging pipe 13 to move downwards, the discharging pipe 13 is separated from the discharging pipe 11, then the motor 6 is started, the output shaft of the motor 6 rotates to drive the rotating shaft 5 to rotate to drive the sphere 9 to rotate, meanwhile, the air inlet machine 18 is opened to inject air into the air inlet pipe 17, and the air is heated in the air inlet pipe 17 through the heating pipe, the heated air enters the air inlet box 16 through the air inlet pipe 17, then enters the cavity 24 through the air inlet hole, and is discharged into the sphere 9 through the exhaust pipe 19 to heat the sphere 9, so that even if the rotating shaft 5 rotates, the injection of hot air is not influenced, the rotating shaft 5 rotates to drive the sphere 9 to rotate, the supernatant in the sphere 9 is firstly driven to move, and simultaneously, all parts of the micro-filtration membrane covered by the inner wall of the sphere 9 are sequentially positioned at the bottom, so that the full utilization of the whole area of the micro-filtration membrane is realized, the situation that water molecules cannot be discharged due to the blockage of the micro-filtration membrane at the bottom can not occur, the injected hot air rapidly dries soybean phospholipids in the sphere 9 to prevent the soybean phospholipids from caking, and the exhaust pipe 19 can stir the supernatant in the rotating process to further prevent the soybean phospholipids from caking, the exhaust pipe 19 and hot air cooperation can make the supernatant that gets into on the spheroid 9 constantly move and discharge through the microfiltration membrane after each position and the microfiltration membrane contact of microfiltration membrane, accelerate the hydrone and pass through the microfiltration membrane, make hydrone exhaust speed faster, hot air that simultaneously pours into can provide pressure, force the hydrone to pass through the microfiltration membrane fast, further accelerate hydrone from the speed of microfiltration membrane exhaust, after the hydrone is discharged more, the effect of wind-force and the stirring effect of exhaust pipe 19 can drive soybean phospholipid constantly fly up the dispersion in spheroid 9, guarantee that soybean phospholipid after the drying is in powder state completely, soybean phospholipid that extracts after the drying of this device can be complete into powder, and do not need to process crushing and stoving again, and because spheroid 9, the rotation of exhaust pipe 19 and hot air's injection, make whole stoving separation's process efficiency higher, very big promotion soybean phospholipid's extraction efficiency, after the stoving is accomplished, motor 6 stops, when spheroid 9 rotates to rest state, start second elevating gear 15 drive discharge pipe 13 and get into valve 11, the exhaust pipe 11 is opened, the soybean phospholipid is gone into in the heat pipe 13 according to the effect of drying, the time, the soybean phospholipid is discharged from the exhaust pipe is discharged into in the volume of the drying pipe 13, the volume is realized in order to realize that the soybean phospholipid is discharged through the exhaust pipe 13, the exhaust pipe is discharged in the volume of the time of the drying pipe is discharged into the volume is in the volume of the top-concrete exhaust pipe 13, the soybean phospholipid is discharged through the exhaust pipe 13, and the air can be separated.
In this embodiment, the feeding valve includes an upper blocking block 26, an upper fixing plate 27, an upper spring 28 and an upper connecting rod 29, the upper fixing plate 27 is fixed on the outer wall of the feeding pipe 10 through the upper connecting rod 29, the upper blocking block 26 movably penetrates through the right wall of the feeding pipe 10, the upper spring 28 is connected between the right wall of the upper blocking block 26 and the left wall of the upper fixing plate 27, the left wall of the upper blocking block 26 is integrally inclined downwards from the right side to the left side, and the bottom end of the feeding pipe 12 moves downwards to prop against the upper blocking block 26 to move to the right side. When the feeding valve needs to be opened, the first lifting device 14 is started to drive the feeding pipe 12 to move downwards to prop against the upper blocking block 26 to move rightwards, so that the feeding pipe 12 and the feeding pipe 10 can be communicated, the feeding valve is arranged, the feeding valve does not need to be opened manually, an electromagnetic valve is not needed, a complicated circuit is not needed to influence the movement of the ball 9, the upper spring 28 can move only by being subjected to larger force, and the tightness of the ball 9 to the feeding pipe 10 in the rotating process is guaranteed.
In this embodiment, the discharging valve includes lower shutoff piece 30, lower fixed plate 31, lower spring 32 and lower connecting rod 33, lower fixed plate 31 is fixed in the outer wall of discharging pipe 11 through lower connecting rod 33, and lower shutoff piece 30 activity runs through the right wall of discharging pipe 11, is connected with lower spring 32 between the right wall of lower shutoff piece 30 and the left wall of lower fixed plate 31, and the left wall of lower shutoff piece 30 is whole downward sloping from left side to the direction on right side, and the top upward motion of discharging pipe 13 can support lower shutoff piece 30 right side motion. When the discharging valve is required to be opened, the second lifting device 15 is started to drive the discharging pipe 13 to move upwards to prop against the lower blocking block 30 to move rightwards, so that the communication between the discharging pipe 13 and the discharging pipe 11 can be realized, the discharging valve is arranged, the discharging valve does not need to be opened manually, an electromagnetic valve is not required, a complicated circuit is not required to influence the movement of the ball 9, the lower spring 32 is required to be subjected to larger force to move, and the tightness of the ball 9 to the discharging pipe 11 is ensured in the rotating process.
In this embodiment, the left wall of the middle cavity is provided with a driving device 20, an output end of the driving device 20 is connected with a fixing plate 21, the driving device 20 can drive the fixing plate 21 to move left and right, two mounting plates 22 are arranged on the fixing plate 21, the two mounting plates 22 are distributed along the front and rear directions, clamping units are arranged on the mounting plates 22, and the clamping units on the two mounting plates 22 can clamp the leftmost discharging pipe 11.
In this embodiment, the clamping unit includes an air cylinder and a clamping plate 23, the air cylinder is fixed on the mounting plate 22, the output end of the air cylinder is connected with the clamping plate 23, and the two clamping plates 23 can clamp the discharging pipe 11.
When spheroid 9 rotates the in-process, mounting panel 22 and clamping unit are located the left side of left side discharging pipe 11, do not influence the rotation of spheroid 9, when the stoving finishes and need the row material, start drive arrangement and drive fixed plate 21 and move to the right, and then drive mounting panel 22 and clamping unit and move to the right, then start two cylinders, drive two splint 23 and move in opposite directions and carry out the centre gripping to left side discharging pipe 11, thereby realize the location of spheroid 9, in the discharging pipe 11 of follow-up discharging pipe 13 gets into of being convenient for, and the pan feeding pipe 12 gets into in the inlet pipe 10 when the supernatant fluid is poured into in the follow-up.
The exhaust pipe set in this embodiment includes a plurality of exhaust pipes 19, and the plurality of exhaust pipes 19 in the exhaust pipe set are distributed around the circumference of the rotating shaft 5.
In this embodiment, the exhaust pipes 19 in the exhaust pipe group are uniformly distributed around the circumference of the rotating shaft 5.
Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present invention, and not for limiting the same; although the invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some or all of the technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit of the invention, and are intended to be included within the scope of the appended claims and description.
Claims (7)
1. The process for extracting the enzymatic hydrolysis soybean phospholipids is characterized by comprising the following steps of:
A. screening full soybeans, cleaning the soybeans, and standing and drying;
B. heating and crushing the dried soybeans, mixing the crushed soybeans with water to obtain a mixed solution, adding alkaline protease into the mixed solution for enzymolysis, and centrifuging after enzymolysis to obtain free oil, emulsion, hydrolysate and residues;
C. adding carboxylic acid hydrolase into the emulsion obtained in the step B for enzymolysis, and then adding water for hydration to obtain supernatant;
D. drying and filtering the supernatant liquid simultaneously by using a microfiltration membrane filter to obtain soybean phospholipid;
the microfiltration membrane filter comprises a box body (1) with an open top and a plurality of drying and filtering units, wherein an upper partition plate (2), a middle partition plate (3) and a lower partition plate (4) are arranged in the box body (1), the upper partition plate (2), the middle partition plate (3) and the lower partition plate (4) divide the inner cavity of the box body into a top cavity, an upper cavity, a middle cavity and a lower cavity, a rotating shaft (5) is rotatably arranged in the middle cavity, a second gear (8) is sleeved on the rotating shaft (5), a motor (6) is arranged in the middle cavity, a first gear (7) is connected to an output shaft of the motor (6), and the first gear (7) is meshed with the second gear (8);
a plurality of drying and filtering units are horizontally distributed, each drying and filtering unit comprises a sphere (9), a feed pipe (10), a discharge pipe (11), a feed pipe (12), a discharge pipe (13), a first lifting device (14), a second lifting device (15), an air inlet box (16), an air inlet pipe (17), an air inlet machine (18), a storage box (25) and a plurality of exhaust pipe groups,
the ball body (9) is fixed on the rotating shaft (5), the rotating shaft (5) penetrates through the ball body (9), a plurality of through holes are formed in the ball body (9), the micro-filtration membrane is covered on the inner wall of the ball body (9), the feeding pipe (10) is fixed at the top end of the ball body (9), the discharging pipe (11) is fixed at the bottom end of the ball body (9), the feeding valve is arranged on the feeding pipe (10), the discharging valve is arranged on the discharging pipe (11),
a plurality of cavities (24) are arranged in the rotating shaft (5), the cavities (24) are in one-to-one correspondence with the spheres (9), one part of the cavities (24) is positioned in the spheres (9), the other part of the cavities (24) is positioned outside the spheres (9), a plurality of exhaust pipe groups are arranged on the rotating shaft (5) and are horizontally distributed, a plurality of exhaust pipe groups are positioned in the spheres (9), at least one exhaust pipe (19) is arranged in each exhaust pipe group, the exhaust pipe (19) is communicated with the cavities (24),
the air inlet box (16) is fixed in the middle cavity through the supporting component, the rotating shaft (5) rotates to penetrate through the left wall and the right wall of the air inlet box (16), the air inlet box (16) is positioned outside the sphere (9), the side wall of the cavity (24) is provided with an air inlet hole, the air inlet hole is positioned in the inner cavity of the air inlet box (16), the output end of the air inlet machine (18) is connected with the air inlet pipe (17), a heating pipe is arranged in the air inlet pipe (17), the air inlet pipe (17) is communicated with the inner cavity of the air inlet box (16),
the upper partition plate (2) is provided with a plurality of liquid discharge holes, the liquid discharge holes are in one-to-one correspondence with the feeding pipes (12), the first lifting device (14) is arranged in the upper cavity, the output end of the first lifting device (14) is connected with the feeding pipes (12), the top ends of the feeding pipes (12) enter the liquid discharge holes, the bottom ends of the feeding pipes (12) penetrate through the middle partition plate (3) to enter the middle cavity, and the bottom ends of the feeding pipes (12) can enter the feeding pipes (10);
the storage box (25) and the second lifting device (15) are arranged in the lower cavity, the output end of the second lifting device (15) is connected with the discharge pipe (13), the bottom end of the discharge pipe (13) is located in the storage box (25), the top end of the discharge pipe (13) penetrates through the lower partition plate (4) to enter the middle cavity, and the top end of the discharge pipe (13) can enter the discharge pipe (11).
2. The process for extracting enzymatic hydrolysis soybean phospholipids according to claim 1, wherein the feed valve comprises an upper blocking block (26), an upper fixing plate (27), an upper spring (28) and an upper connecting rod (29), the upper fixing plate (27) is fixed on the outer wall of the feed pipe (10) through the upper connecting rod (29), the upper blocking block (26) movably penetrates through the right wall of the feed pipe (10), the upper spring (28) is connected between the right wall of the upper blocking block (26) and the left wall of the upper fixing plate (27), the left wall of the upper blocking block (26) is integrally inclined downwards in the direction from the right side to the left side, and the bottom end of the feed pipe (12) moves downwards to abut against the upper blocking block (26) to move to the right side.
3. The process for extracting the enzymatic hydrolysis soybean phospholipids according to claim 1, wherein the discharge valve comprises a lower blocking block (30), a lower fixing plate (31), a lower spring (32) and a lower connecting rod (33), the lower fixing plate (31) is fixed on the outer wall of the discharge pipe (11) through the lower connecting rod (33), the lower blocking block (30) movably penetrates through the right wall of the discharge pipe (11), the lower spring (32) is connected between the right wall of the lower blocking block (30) and the left wall of the lower fixing plate (31), the left wall of the lower blocking block (30) integrally inclines downwards in the direction from left side to right side, and the top end of the discharge pipe (13) moves upwards to resist the lower blocking block (30) to move to the right side.
4. The process for extracting enzymatic hydrolysis soybean phospholipids according to claim 1, characterized in that a driving device (20) is arranged on the left wall of the middle cavity, the output end of the driving device (20) is connected with a fixed plate (21), the driving device (20) can drive the fixed plate (21) to move left and right, two mounting plates (22) are arranged on the fixed plate (21), the two mounting plates (22) are distributed along the front and rear directions, clamping units are arranged on the mounting plates (22), and the clamping units on the two mounting plates (22) can clamp the leftmost discharging pipe (11).
5. The process for extracting enzymatic hydrolysis soybean phospholipids according to claim 4, wherein the clamping unit comprises a cylinder and clamping plates (23), the cylinder is fixed on the mounting plate (22), the output end of the cylinder is connected with the clamping plates (23), and the two clamping plates (23) can clamp the discharge pipe (11).
6. A process for extracting enzymatic hydrolysis soybean phospholipids according to claim 1, characterized in that the exhaust stack comprises a plurality of exhaust pipes (19), the exhaust pipes (19) in the exhaust stack being circumferentially distributed around the rotating shaft (5).
7. A process for extracting enzymatic hydrolysis soybean phospholipids according to claim 1, characterized in that the exhaust pipes (19) in the exhaust pipe group are uniformly distributed around the circumference of the rotating shaft (5).
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| CN202210085101.8A CN114478621B (en) | 2022-01-25 | 2022-01-25 | Process for extracting enzymatic soybean phospholipids |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1733775A (en) * | 2005-08-10 | 2006-02-15 | 武汉湖工大膜技术开发有限公司 | Method for phosphatide extraction from soybean vegetable oil |
| CN102827685A (en) * | 2012-09-17 | 2012-12-19 | 东北农业大学 | Method for extracting soybean phospholipids through aqueous enzymatic method |
| KR101712453B1 (en) * | 2016-05-31 | 2017-03-07 | 주식회사 토디팜코리아 | Method for preparing bean curd with nipa fruticans wurmb and bean curd by the method |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2901488B1 (en) * | 2006-05-23 | 2008-08-15 | Otv Sa | AERATION DEVICE FOR IMMERSION MEMBRANE-BASED WATER FILTRATION SYSTEM INCLUDING FLOOR WITH GAS INJECTION MEANS AND AT LEAST ONE PRESSURE BALANCING SYSTEM |
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2022
- 2022-01-25 CN CN202210085101.8A patent/CN114478621B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1733775A (en) * | 2005-08-10 | 2006-02-15 | 武汉湖工大膜技术开发有限公司 | Method for phosphatide extraction from soybean vegetable oil |
| CN102827685A (en) * | 2012-09-17 | 2012-12-19 | 东北农业大学 | Method for extracting soybean phospholipids through aqueous enzymatic method |
| KR101712453B1 (en) * | 2016-05-31 | 2017-03-07 | 주식회사 토디팜코리아 | Method for preparing bean curd with nipa fruticans wurmb and bean curd by the method |
Non-Patent Citations (1)
| Title |
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| 大豆中磷脂的提取技术研究;高林霞等;安全与环境工程;20060630;13(2);第98-99, 102页 * |
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