CN114478621A - Process for extracting enzymolysis soybean phospholipid - Google Patents
Process for extracting enzymolysis soybean phospholipid Download PDFInfo
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- CN114478621A CN114478621A CN202210085101.8A CN202210085101A CN114478621A CN 114478621 A CN114478621 A CN 114478621A CN 202210085101 A CN202210085101 A CN 202210085101A CN 114478621 A CN114478621 A CN 114478621A
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- 239000008347 soybean phospholipid Substances 0.000 title claims abstract description 31
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- 230000008569 process Effects 0.000 title claims abstract description 24
- 235000010469 Glycine max Nutrition 0.000 claims abstract description 41
- 244000068988 Glycine max Species 0.000 claims abstract description 39
- 238000001471 micro-filtration Methods 0.000 claims abstract description 30
- 239000012528 membrane Substances 0.000 claims abstract description 29
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 23
- 239000006228 supernatant Substances 0.000 claims abstract description 21
- 238000001035 drying Methods 0.000 claims abstract description 20
- 238000001914 filtration Methods 0.000 claims abstract description 14
- 229940083466 soybean lecithin Drugs 0.000 claims abstract description 14
- 239000000839 emulsion Substances 0.000 claims abstract description 12
- 238000010438 heat treatment Methods 0.000 claims abstract description 10
- 239000011259 mixed solution Substances 0.000 claims abstract description 10
- 108091005658 Basic proteases Proteins 0.000 claims abstract description 8
- 239000003921 oil Substances 0.000 claims abstract description 8
- 238000002156 mixing Methods 0.000 claims abstract description 7
- 238000000926 separation method Methods 0.000 claims abstract description 7
- 230000036571 hydration Effects 0.000 claims abstract description 6
- 238000006703 hydration reaction Methods 0.000 claims abstract description 6
- 239000000413 hydrolysate Substances 0.000 claims abstract description 6
- 108090000604 Hydrolases Proteins 0.000 claims abstract description 5
- 102000004157 Hydrolases Human genes 0.000 claims abstract description 5
- 238000004140 cleaning Methods 0.000 claims abstract description 4
- 238000012216 screening Methods 0.000 claims abstract description 4
- 238000007599 discharging Methods 0.000 claims description 48
- 238000005192 partition Methods 0.000 claims description 27
- 239000000463 material Substances 0.000 claims description 23
- 230000000903 blocking effect Effects 0.000 claims description 14
- 239000007788 liquid Substances 0.000 claims description 11
- 230000003028 elevating effect Effects 0.000 claims description 7
- 238000003860 storage Methods 0.000 claims description 5
- 230000002255 enzymatic effect Effects 0.000 claims 4
- 239000000843 powder Substances 0.000 abstract description 16
- 230000033001 locomotion Effects 0.000 description 11
- 235000019198 oils Nutrition 0.000 description 6
- 239000000203 mixture Substances 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 230000009471 action Effects 0.000 description 3
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- 150000003904 phospholipids Chemical class 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- PORPENFLTBBHSG-MGBGTMOVSA-N 1,2-dihexadecanoyl-sn-glycerol-3-phosphate Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP(O)(O)=O)OC(=O)CCCCCCCCCCCCCCC PORPENFLTBBHSG-MGBGTMOVSA-N 0.000 description 1
- TZCPCKNHXULUIY-RGULYWFUSA-N 1,2-distearoyl-sn-glycero-3-phosphoserine Chemical compound CCCCCCCCCCCCCCCCCC(=O)OC[C@H](COP(O)(=O)OC[C@H](N)C(O)=O)OC(=O)CCCCCCCCCCCCCCCCC TZCPCKNHXULUIY-RGULYWFUSA-N 0.000 description 1
- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 description 1
- FGRBYDKOBBBPOI-UHFFFAOYSA-N 10,10-dioxo-2-[4-(N-phenylanilino)phenyl]thioxanthen-9-one Chemical compound O=C1c2ccccc2S(=O)(=O)c2ccc(cc12)-c1ccc(cc1)N(c1ccccc1)c1ccccc1 FGRBYDKOBBBPOI-UHFFFAOYSA-N 0.000 description 1
- 102000013392 Carboxylesterase Human genes 0.000 description 1
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- ZWZWYGMENQVNFU-UHFFFAOYSA-N Glycerophosphorylserin Natural products OC(=O)C(N)COP(O)(=O)OCC(O)CO ZWZWYGMENQVNFU-UHFFFAOYSA-N 0.000 description 1
- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 description 1
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- 238000006243 chemical reaction Methods 0.000 description 1
- OEYIOHPDSNJKLS-UHFFFAOYSA-N choline Chemical compound C[N+](C)(C)CCO OEYIOHPDSNJKLS-UHFFFAOYSA-N 0.000 description 1
- 229960001231 choline Drugs 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
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- 230000007547 defect Effects 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 238000004945 emulsification Methods 0.000 description 1
- 230000007071 enzymatic hydrolysis Effects 0.000 description 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 229960000367 inositol Drugs 0.000 description 1
- -1 inositol phospholipids Chemical class 0.000 description 1
- 229940067606 lecithin Drugs 0.000 description 1
- 239000000787 lecithin Substances 0.000 description 1
- 235000010445 lecithin Nutrition 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005374 membrane filtration Methods 0.000 description 1
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- 238000012986 modification Methods 0.000 description 1
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- 239000012454 non-polar solvent Substances 0.000 description 1
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- 238000007254 oxidation reaction Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
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- 230000001737 promoting effect Effects 0.000 description 1
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 235000012424 soybean oil Nutrition 0.000 description 1
- 239000003549 soybean oil Substances 0.000 description 1
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- 238000003756 stirring Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
- C07F9/02—Phosphorus compounds
- C07F9/06—Phosphorus compounds without P—C bonds
- C07F9/08—Esters of oxyacids of phosphorus
- C07F9/09—Esters of phosphoric acids
- C07F9/10—Phosphatides, e.g. lecithin
- C07F9/103—Extraction or purification by physical or chemical treatment of natural phosphatides; Preparation of compositions containing phosphatides of unknown structure
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Beans For Foods Or Fodder (AREA)
Abstract
The invention discloses a process for extracting enzymolysis soybean lecithin, which comprises the following steps: A. screening plump soybeans, cleaning the soybeans, and standing for drying; B. heating and crushing the dried soybeans, mixing the crushed soybeans with water to obtain a mixed solution, adding alkaline protease into the mixed solution for enzymolysis, and performing centrifugal separation after enzymolysis to obtain free oil, emulsion, hydrolysate and residues; C. b, adding carboxylic acid hydrolase into the emulsion obtained in the step B for enzymolysis, and adding water for hydration to obtain supernate; D. drying and filtering the supernatant by using a microfiltration membrane filter to obtain soybean phospholipids; the process can make soybean powder fully react during enzymolysis, and improve the utilization rate of the components in the soybean.
Description
Technical Field
The invention relates to the technical field of extraction of soybean phospholipid, and in particular relates to a process for extracting enzymolysis soybean phospholipid.
Background
Soy phospholipids are products extracted from the oil foot from the production of soybean oil, are esters of glycerol, fatty acids, choline or cholamine, and are soluble in oils and nonpolar solvents. Soy phospholipids are complex in composition and contain mostly lecithin (about 34.2%), cephalins (about 19.7%), inositol phospholipids (about 16.0%), phosphatidylserine (about 15.8%), phosphatidic acid (about 3.6%) and other phospholipids (about 10.7%). As a pale yellow to brown viscous liquid or as a white to pale brown solid powder.
The soybean lecithin not only has stronger emulsification, moistening and dispersing functions, but also plays an important role in promoting fat metabolism in vivo, muscle growth, nervous system development, oxidation damage resistance in vivo and the like.
At present, in the process of extracting soybeans by adopting an enzymatic hydrolysis method, the problem of insufficient reaction between soybean powder and enzyme often occurs, so that the ingredients in the soybeans are wasted.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides the process for extracting the soybean lecithin by enzymolysis, which can ensure that soybean powder fully reacts during enzymolysis and improve the utilization rate of the components in the soybeans.
A process for extracting enzymolysis soybean phospholipid comprises the following steps:
A. screening plump soybeans, cleaning the soybeans, and standing for drying;
B. heating and crushing the dried soybeans, mixing the crushed soybeans with water to obtain a mixed solution, adding alkaline protease into the mixed solution for enzymolysis, and performing centrifugal separation after enzymolysis to obtain free oil, emulsion, hydrolysate and residues;
C. b, adding carboxylic acid hydrolase into the emulsion obtained in the step B for enzymolysis, and adding water for hydration to obtain supernatant;
D. and (4) simultaneously drying and filtering the supernatant by using a microfiltration membrane filter to obtain the soybean phospholipids.
Preferably, the microfiltration membrane filter comprises a box body with an open top and a plurality of drying and filtering units, an upper partition plate, a middle partition plate and a lower partition plate are arranged in the box body, an inner cavity of the box body is divided into a top cavity, an upper cavity, a middle cavity and a lower cavity by the upper partition plate, the middle partition plate and the lower partition plate, a rotating shaft is rotatably arranged in the middle cavity, a second gear is sleeved on the rotating shaft, a motor is arranged in the middle cavity, a first gear is connected to an output shaft of the motor, and the first gear is meshed with the second gear;
the drying and filtering units are distributed horizontally and comprise spheres, a feeding pipe, a discharging pipe, a first lifting device, a second lifting device, an air inlet box, an air inlet pipe, an air inlet machine, a storage box and a plurality of air exhaust pipe groups,
the ball body is fixed on the rotating shaft, the rotating shaft penetrates through the ball body, a plurality of through holes are formed in the ball body, the inner wall of the ball body is covered with a microfiltration membrane, the feeding pipe is fixed at the top end of the ball body, the discharging pipe is fixed at the bottom end of the ball body, a feeding valve is arranged on the feeding pipe, a discharging valve is arranged on the discharging pipe,
a plurality of cavities are arranged in the rotating shaft, the cavities correspond to the spheres one by one, one part of the cavities is positioned in the spheres, the other part of the cavities is positioned outside the spheres, a plurality of exhaust pipe groups are all arranged on the rotating shaft and are distributed horizontally, a plurality of exhaust pipe groups are all positioned in the spheres and are provided with at least one exhaust pipe, the exhaust pipes are communicated with the cavities,
the air inlet box is fixed in the middle cavity through the supporting component, the rotating shaft rotatably penetrates through the left wall and the right wall of the air inlet box, the air inlet box is positioned outside the sphere, the side wall of the cavity is provided with an air inlet hole, the air inlet hole is positioned in the inner cavity of the air inlet box, the output end of the air inlet machine is connected with an air inlet pipe, a heating pipe is arranged in the air inlet pipe, the air inlet pipe is communicated with the inner cavity of the air inlet box,
the upper partition plate is provided with a plurality of liquid discharge holes, the liquid discharge holes correspond to the feeding pipes one by one, the first lifting device is arranged in the upper cavity, the output end of the first lifting device is connected with the feeding pipes, the top ends of the feeding pipes enter the liquid discharge holes, the bottom ends of the feeding pipes penetrate through the middle partition plate and enter the middle cavity, and the bottom ends of the feeding pipes can enter the feeding pipes;
the receiver all sets up in the intracavity down with second elevating gear, and second elevating gear's output and row material union coupling arrange the bottom of material pipe and be located the receiver box, arrange the top of material pipe and pass down the baffle and get into the cavity, arrange the material pipe top and can get into in the discharging tube.
Preferably, feed valve includes plugging block, an upper fixed plate, top spring and last connecting rod, an upper fixed plate is fixed in the outer wall of inlet pipe through last connecting rod, goes up the right wall that plugging block activity runs through the inlet pipe, is connected with the top spring between the right wall of last plugging block and an upper fixed plate's the left wall, goes up the left wall of plugging block and follows the right side and whole downward sloping in left direction, and the bottom downstream of pan feeding pipe can support and hold the motion of plugging block to the right side.
Preferably, discharge valve includes lower plugging block, bottom plate, lower spring and lower connecting rod, the bottom plate is fixed in the outer wall of discharging pipe through lower connecting rod, and the right wall of discharging pipe is run through in lower plugging block activity, is connected with the lower spring between the right wall of lower plugging block and the left wall of bottom plate, and the left wall of lower plugging block is followed the left side and is gone up whole downward sloping in the direction on the right side, and the top upward movement of arranging the material pipe can support and hold down plugging block to the right side motion.
Preferably, the left wall of the middle cavity is provided with a driving device, the output end of the driving device is connected with a fixing plate, the driving device can drive the fixing plate to move left and right, the fixing plate is provided with two mounting plates, the two mounting plates are distributed along the front and back direction, the mounting plates are provided with clamping units, and the clamping units on the two mounting plates can clamp the discharge pipe at the leftmost side.
Preferably, the clamping unit includes cylinder and splint, and the cylinder is fixed on the mounting panel, and the output and the splint of cylinder are connected, and two splint can carry out the centre gripping to the discharging pipe.
Preferably, the exhaust pipe group comprises a plurality of exhaust pipes, and the plurality of exhaust pipes in the exhaust pipe group are distributed around the circumference of the rotating shaft.
Preferably, a plurality of exhaust ducts in the exhaust duct group are uniformly distributed around the circumference of the rotating shaft.
The invention has the beneficial effects that: in the technical scheme, soybeans with plump particles are screened, cleaned and placed for drying; then heating and crushing the dried soybeans, mixing the crushed soybeans with water to obtain a mixed solution, adding alkaline protease into the mixed solution for enzymolysis, and performing centrifugal separation after enzymolysis to obtain free oil, emulsion, hydrolysate and residues; adding carboxylic acid hydrolase into the obtained emulsion for enzymolysis, and adding water for hydration to obtain supernatant; finally, simultaneously drying and filtering the supernatant by using a microfiltration membrane filter to obtain soybean lecithin; carry out kibbling in-process to the soybean among this technical scheme and dry simultaneously, guarantee that the soybean powder that forms can not agglomerate, the soybean powder can be abundant mix with water when mixing with water like this, then add alkaline protease and carry out the enzymolysis and can make the abundant enzymolysis of soybean powder, this technology can be so that the soybean powder fully reacts when the enzymolysis, improves the utilization ratio of composition in the soybean.
Drawings
In order to more clearly illustrate the detailed description of the invention or the technical solutions in the prior art, the drawings that are needed in the detailed description of the invention or the prior art will be briefly described below. Throughout the drawings, like elements or portions are generally identified by like reference numerals. In the drawings, elements or portions are not necessarily drawn to scale.
FIG. 1 is a front sectional view of the present invention;
FIG. 2 is an enlarged schematic view of the position A in FIG. 1;
fig. 3 is an enlarged schematic view of a position B in fig. 1.
In the figure, 1-box, 2-upper partition, 3-middle partition, 4-lower partition, 5-shaft, 6-motor, 7-first gear, 8-second gear, 9-sphere, 10-inlet pipe, 11-outlet pipe, 12-inlet pipe, 13-outlet pipe, 14-first lifting device, 15-second lifting device, 16-inlet box, 17-inlet pipe, 18-inlet fan, 19-outlet pipe, 20-driving device, 21-fixed plate, 22-mounting plate, 23-clamping plate, 24-cavity, 25-storage box, 26-upper block, 27-upper fixed plate, 28-upper spring, 29-upper connecting rod, 30-lower block, 31-lower fixed plate, 32-lower spring, 33-lower connecting rod.
Detailed Description
Embodiments of the present invention will be described in detail below with reference to the accompanying drawings. The following examples are only for illustrating the technical solutions of the present invention more clearly, and therefore are only examples, and the protection scope of the present invention is not limited thereby.
It is to be noted that, unless otherwise specified, technical or scientific terms used herein shall have the ordinary meaning as understood by those skilled in the art to which the invention pertains.
Example 1
As shown in fig. 1 to fig. 3, the present embodiment provides a process for extracting enzymatically hydrolyzed soybean phospholipids, which includes the following steps:
A. screening plump soybeans, cleaning the soybeans, and standing and drying;
B. heating and crushing the dried soybeans, mixing the crushed soybeans with water to obtain a mixed solution, adding alkaline protease into the mixed solution for enzymolysis, and performing centrifugal separation after enzymolysis to obtain free oil, emulsion, hydrolysate and residues;
C. b, adding carboxylic acid hydrolase into the emulsion obtained in the step B for enzymolysis, and adding water for hydration to obtain supernatant;
D. and (4) simultaneously drying and filtering the supernatant by using a microfiltration membrane filter to obtain the soybean phospholipids.
In the traditional process for extracting soybean phospholipid by enzymolysis, soybeans are crushed into powder, water is added into the powder to be mixed to obtain mixed liquid, then alkaline protease is added to perform enzymolysis to separate out free oil, emulsion, hydrolysate and residues, then the emulsion is subjected to enzymolysis by using carboxylesterase, supernatant is obtained after hydration, and the supernatant is subjected to microfiltration membrane filtration to obtain the soybean phospholipid.
Carry out kibbling in-process to the soybean and dry simultaneously in this embodiment, guarantee that the soybean powder that forms can not agglomerate, soybean powder can be abundant mix with water when mixing with water like this, then add alkaline protease and carry out the enzymolysis and can make the abundant enzymolysis of soybean powder, this technology can be so that soybean powder fully reacts when the enzymolysis, improves the utilization ratio of composition in the soybean.
The microfiltration membrane filter comprises a box body 1 with an open top and a plurality of drying and filtering units, wherein an upper partition plate 2, a middle partition plate 3 and a lower partition plate 4 are arranged in the box body 1, the inner cavity of the box body is divided into a top cavity, an upper cavity, a middle cavity and a lower cavity by the upper partition plate 2, the middle partition plate 3 and the lower partition plate 4, a rotating shaft 5 is rotatably arranged in the middle cavity, a second gear 8 is sleeved on the rotating shaft 5, a motor 6 is arranged in the middle cavity, a first gear 7 is connected to an output shaft of the motor 6, and the first gear 7 is meshed with the second gear 8;
the drying and filtering units are distributed horizontally, each drying and filtering unit comprises a sphere 9, a feeding pipe 10, a discharging pipe 11, a feeding pipe 12, a discharging pipe 13, a first lifting device 14, a second lifting device 15, an air inlet box 16, an air inlet pipe 17, an air inlet machine 18, a storage box 25 and a plurality of air exhaust pipe groups,
the ball body 9 is fixed on the rotating shaft 5, the rotating shaft 5 penetrates through the ball body 9, a plurality of through holes are formed in the ball body 9, the inner wall of the ball body 9 is covered with a microfiltration membrane, the feeding pipe 10 is fixed at the top end of the ball body 9, the discharging pipe 11 is fixed at the bottom end of the ball body 9, the feeding pipe 10 is provided with a feeding valve, the discharging pipe 11 is provided with a discharging valve,
the rotating shaft 5 is internally provided with a plurality of cavities 24, the cavities 24 correspond to the spheres 9 one by one, one part of the cavity 24 is positioned in the sphere 9, the other part of the cavity 24 is positioned outside the sphere 9, a plurality of exhaust pipe groups are all arranged on the rotating shaft 5 and are distributed horizontally, a plurality of exhaust pipe groups are all positioned in the sphere 9, at least one exhaust pipe 19 is arranged in each exhaust pipe group, the exhaust pipes 19 are communicated with the cavities 24,
the air inlet box 16 is fixed in the middle cavity through a support component, the rotating shaft 5 rotatably penetrates through the left wall and the right wall of the air inlet box 16, the air inlet box 16 is positioned outside the sphere 9, the side wall of the cavity 24 is provided with an air inlet hole which is positioned in the inner cavity of the air inlet box 16, the output end of the air inlet machine 18 is connected with an air inlet pipe 17, a heating pipe is arranged in the air inlet pipe 17, the air inlet pipe 17 is communicated with the inner cavity of the air inlet box 16,
the upper partition plate 2 is provided with a plurality of liquid discharge holes, the liquid discharge holes correspond to the feeding pipes 12 one by one, the first lifting device 14 is arranged in the upper cavity, the output end of the first lifting device 14 is connected with the feeding pipes 12, the top ends of the feeding pipes 12 enter the liquid discharge holes, the bottom ends of the feeding pipes 12 penetrate through the middle partition plate 3 and enter the middle cavity, and the bottom ends of the feeding pipes 12 can enter the feeding pipes 10;
In the process of extracting the soybean phospholipids, a microfiltration membrane is adopted for filtration in the last step, the soybean phospholipids are separated from the supernatant, in the step, the current working mode is to lay the microfiltration membrane on a filter plate, then the supernatant is poured above the microfiltration membrane, so that the moisture passes through the microfiltration membrane, and the soybean phospholipids are positioned on the microfiltration membrane, thereby realizing the separation of the soybean phospholipids, and the method has the problems that 1. when a large amount of soybean phospholipids are covered on the microfiltration membrane, the soybean phospholipids cover the pores of the microfiltration membrane, and the moisture in the supernatant can not be discharged at the moment, so that the further filtration can be realized by manual loosening. 2. When soybean lecithin accumulated on microfiltration membrane, because moisture can not be quick stoving, soybean lecithin also can agglomerate this moment, and blocking to microfiltration membrane after the agglomeration is more serious, and follow-up needs are smashed and are dried soybean lecithin alone again simultaneously to carrying out the breakage to the soybean lecithin of agglomerating, guaranteeing that soybean lecithin is powdered, consequently adopt traditional mode to extract at the in-process of soybean lecithin extraction efficiency very low.
In this embodiment, an upper partition plate 2, a middle partition plate 3 and a lower partition plate 4 are arranged in the box body 1, the inner cavity of the box body is divided into a top cavity, an upper cavity, a middle cavity and a lower cavity by the upper partition plate 2, the middle partition plate 3 and the lower partition plate 4, the motor 6 can drive the rotating shaft 5 to rotate, and the motor 6 can idle when not working.
The specific process principle is as follows, when in an initial state, the bottom end of a feeding pipe 12 enters a feeding pipe 10, the top end of a discharging pipe 13 enters a discharging pipe 11, a feeding valve is in an open state, a discharging valve is in a closed state, supernatant is added into a top cavity at the moment, the supernatant enters a sphere 9 after passing through the feeding pipe 12 and the feeding pipe 10, then the feeding valve is closed, a first lifting device 14 is started to drive the feeding pipe 12 to move upwards, the feeding pipe 12 is separated from the feeding pipe 10, a second lifting device 15 is started to drive the discharging pipe 13 to move downwards, the discharging pipe 13 is separated from the discharging pipe 11, then a motor 6 is started, an output shaft of the motor 6 rotates to drive a rotating shaft 5 to rotate, the rotating shaft 5 rotates to drive the sphere 9 to rotate, an air inlet machine 18 is started simultaneously, the air inlet machine 18 injects air into an air inlet pipe 17, the air is heated in the air inlet pipe 17 through a heating pipe, and the heated air inlet pipe 17 enters an air inlet box 16, then enters the cavity 24 through the air inlet, and is discharged into the sphere 9 through the exhaust pipe 19 to heat the sphere 9, so that the injection of hot air is not influenced even if the rotating shaft 5 rotates, the supernatant in the sphere 9 is firstly driven to move in the process that the rotating shaft 5 rotates to drive the sphere 9 to rotate, meanwhile, each part of the microfiltration membrane covered on the inner wall of the sphere 9 is positioned at the bottom in turn, the full utilization of the whole area of the microfiltration membrane is realized, the condition that water molecules cannot be discharged due to the blockage of the microfiltration membrane at the bottom is avoided, meanwhile, the soybean phospholipid in the sphere 9 is quickly dried by the injected hot air to prevent the soybean phospholipid from caking, the supernatant can be stirred in the rotating process of the exhaust pipe 19, the soybean phospholipid is further prevented from caking, the exhaust pipe 19 and the hot air are matched to enable the supernatant entering the sphere 9 to continuously move to be contacted with the microfiltration membrane at each position of the microfiltration membrane and then discharged through the microfiltration membrane, accelerating the water molecules to pass through the microfiltration membrane, enabling the water molecule discharging speed to be higher, meanwhile, the injected hot air can provide pressure, forcing the water molecules to rapidly pass through the microfiltration membrane, further accelerating the water molecule discharging speed from the microfiltration membrane, after the water molecules are more discharged, the soybean phospholipids can be driven to continuously fly and disperse in the sphere 9 under the action of wind force and the stirring action of the exhaust pipe 19, ensuring that the dried soybean phospholipids are completely in a powder state, the soybean phospholipids extracted after being dried and dried by the device can be completely in a powder state, and the processing, crushing and drying are not needed, and because the sphere 9, the rotation of the exhaust pipe 19 and the injection of the hot air, the efficiency of the whole drying and separating process is higher, the extraction efficiency of the soybean phospholipids is greatly improved, after the drying is finished, the motor 6 stops, at the moment, when the sphere 9 rotates to a static state, the second lifting device 15 is started to drive the material discharging pipe 13 to enter the material discharging pipe 11, the material discharging valve is opened, soybean lecithin in the ball body 9 falls down through the material discharging pipe 11 and the material discharging pipe 13 under the action of gravity to enter the storage box 25, and discharged water in the ball body 9 enters the middle cavity and then is discharged, so that the separation of the soybean lecithin and water molecules is realized, and when the device is used specifically, the wind power of the air inlet machine 18 and the power of the heating pipe can be adjusted according to the amount of injected supernatant.
In this embodiment the feed valve includes plugging block 26, an upper fixed plate 27, goes up spring 28 and last connecting rod 29, upper fixed plate 27 is fixed in the outer wall of inlet pipe 10 through last connecting rod 29, goes up the right wall that plugging block 26 activity runs through inlet pipe 10, goes up and is connected with spring 28 between the right wall of plugging block 26 and the left wall of upper fixed plate 27, goes up the left wall of plugging block 26 and follows the whole downward sloping in left side direction from the right side, and the bottom downstream of pan feeding pipe 12 can support and hold the motion of plugging block 26 to the right side. When the feeding valve needs to be opened, the first lifting device 14 is started to drive the feeding pipe 12 to move downwards to abut against the upper blocking block 26 to move rightwards, so that the feeding pipe 12 and the feeding pipe 10 can be communicated, the feeding valve does not need to be manually opened, an electromagnetic valve does not need to be adopted, a complex circuit is not needed to influence the movement of the ball body 9, the upper spring 28 needs to be moved by a large force, and the sealing performance of the ball body 9 on the rotating process to the feeding pipe 10 is guaranteed.
In this embodiment the discharge valve includes lower shutoff piece 30, bottom plate 31, lower spring 32 and lower connecting rod 33, bottom plate 31 is fixed in the outer wall of discharging pipe 11 through lower connecting rod 33, and the activity of lower shutoff piece 30 runs through the right wall of discharging pipe 11, is connected with lower spring 32 between the right wall of lower shutoff piece 30 and the left wall of bottom plate 31, and the left wall of lower shutoff piece 30 is followed the left side and is gone up whole downward sloping to the direction on the right side, and the top upward movement of arranging material pipe 13 can support and hold lower shutoff piece 30 and move to the right side. When the discharge valve is opened to needs, start second elevating gear 15 and drive row material pipe 13 upward movement and support blocking piece 30 and move right under holding and can realize arranging the intercommunication of material pipe 13 and discharging pipe 11, so set up the discharge valve and do not need the manual work to open, also need not adopt the solenoid valve, do not need complicated circuit to influence the motion of spheroid 9, here lower spring 32 need receive great power just can the motion, guarantee spheroid 9 leakproofness to discharging pipe 11 of blocking piece 30 under rotatory in-process like this.
In this embodiment the lumen left wall is provided with drive arrangement 20, and drive arrangement 20's output is connected with fixed plate 21, and drive arrangement 20 can drive fixed plate 21 side-to-side motion, is provided with two mounting panels 22 on the fixed plate 21, and two mounting panels 22 distribute along the fore-and-aft direction, are provided with the centre gripping unit on the mounting panel 22, and the centre gripping unit on two mounting panels 22 can carry out the centre gripping to leftmost discharging pipe 11.
In this embodiment the clamping unit includes cylinder and splint 23, and the cylinder is fixed on mounting panel 22, and the output and the splint 23 of cylinder are connected, and two splint 23 can carry out the centre gripping to discharging pipe 11.
When spheroid 9 rotates the in-process, mounting panel 22 and centre gripping unit are located the left side of leftmost discharging pipe 11, do not influence spheroid 9's rotation, when the stoving finishes to arrange the material, start drive arrangement and drive fixed plate 21 and move right, and then drive mounting panel 22 and centre gripping unit and move right, then start two cylinders, it carries out the centre gripping to leftmost discharging pipe 11 to drive two splint 23 motion in opposite directions, thereby realize spheroid 9's location, be convenient for follow-up row material pipe 13 gets into in discharging pipe 11, and during follow-up injection supernatant pan feeding pipe 12 gets into in the inlet pipe 10.
The exhaust duct group in this embodiment includes a plurality of exhaust ducts 19, and the plurality of exhaust ducts 19 in the exhaust duct group are distributed around the circumference of the rotating shaft 5.
In this embodiment, the plurality of exhaust ducts 19 in the exhaust duct group are uniformly distributed around the circumference of the rotating shaft 5.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the present invention, and they should be construed as being included in the following claims and description.
Claims (8)
1. A process for extracting enzymolysis soybean phospholipid is characterized by comprising the following steps:
A. screening plump soybeans, cleaning the soybeans, and standing for drying;
B. heating and crushing the dried soybeans, mixing the crushed soybeans with water to obtain a mixed solution, adding alkaline protease into the mixed solution for enzymolysis, and performing centrifugal separation after enzymolysis to obtain free oil, emulsion, hydrolysate and residues;
C. b, adding carboxylic acid hydrolase into the emulsion obtained in the step B for enzymolysis, and adding water for hydration to obtain supernatant;
D. and (4) simultaneously drying and filtering the supernatant by using a microfiltration membrane filter to obtain the soybean phospholipids.
2. The process for extracting enzymatic soybean phospholipid as defined in claim 1, wherein the microfiltration membrane filter comprises a box body (1) with an open top and a plurality of drying and filtering units, an upper baffle (2), a middle baffle (3) and a lower baffle (4) are arranged in the box body (1), the inner cavity of the box body is divided into a top cavity, an upper cavity, a middle cavity and a lower cavity by the upper baffle (2), the middle baffle (3) and the lower baffle (4), a rotating shaft (5) is rotatably arranged in the middle cavity, a second gear (8) is sleeved on the rotating shaft (5), a motor (6) is arranged in the middle cavity, a first gear (7) is connected to an output shaft of the motor (6), and the first gear (7) is meshed with the second gear (8);
the drying and filtering units are distributed horizontally and comprise a sphere (9), a feeding pipe (10), a discharging pipe (11), a feeding pipe (12), a discharging pipe (13), a first lifting device (14), a second lifting device (15), an air inlet box (16), an air inlet pipe (17), an air inlet machine (18), a storage box (25) and a plurality of air exhaust pipe groups,
the ball body (9) is fixed on the rotating shaft (5), the rotating shaft (5) penetrates through the ball body (9), a plurality of through holes are formed in the ball body (9), a micro-filtration membrane covers the inner wall of the ball body (9), the feeding pipe (10) is fixed at the top end of the ball body (9), the discharging pipe (11) is fixed at the bottom end of the ball body (9), a feeding valve is arranged on the feeding pipe (10), a discharging valve is arranged on the discharging pipe (11),
a plurality of cavities (24) are arranged in the rotating shaft (5), the cavities (24) correspond to the spheres (9) one by one, one part of the cavity (24) is positioned in the sphere (9), the other part of the cavity (24) is positioned outside the sphere (9), a plurality of exhaust pipe groups are all arranged on the rotating shaft (5), the exhaust pipe groups are horizontally distributed and are all positioned in the sphere (9), at least one exhaust pipe (19) is arranged in each exhaust pipe group, the exhaust pipes (19) are communicated with the cavities (24),
the air inlet box (16) is fixed in the middle cavity through the supporting component, the rotating shaft (5) rotatably penetrates through the left wall and the right wall of the air inlet box (16), the air inlet box (16) is positioned outside the sphere (9), the side wall of the cavity (24) is provided with an air inlet hole, the air inlet hole is positioned in the inner cavity of the air inlet box (16), the output end of the air inlet machine (18) is connected with an air inlet pipe (17), a heating pipe is arranged in the air inlet pipe (17), the air inlet pipe (17) is communicated with the inner cavity of the air inlet box (16),
a plurality of liquid discharge holes are formed in the upper partition plate (2), the liquid discharge holes correspond to the feeding pipes (12) one by one, the first lifting device (14) is arranged in the upper cavity, the output end of the first lifting device (14) is connected with the feeding pipes (12), the top ends of the feeding pipes (12) enter the liquid discharge holes, the bottom ends of the feeding pipes (12) penetrate through the middle partition plate (3) and enter the middle cavity, and the bottom ends of the feeding pipes (12) can enter the feeding pipes (10);
receiver (25) all set up in the intracavity under with second elevating gear (15), and the output of second elevating gear (15) is connected with row material pipe (13), and the bottom of arranging material pipe (13) is located receiver (25), and baffle (4) get into the intracavity under passing on the top of arranging material pipe (13), arrange material pipe (13) top and can get into discharging pipe (11).
3. The process for extracting enzymatic soybean phospholipid as defined in claim 2, wherein the feeding valve comprises an upper blocking block (26), an upper fixing plate (27), an upper spring (28) and an upper connecting rod (29), the upper fixing plate (27) is fixed on the outer wall of the feeding pipe (10) through the upper connecting rod (29), the upper blocking block (26) movably penetrates through the right wall of the feeding pipe (10), the upper spring (28) is connected between the right wall of the upper blocking block (26) and the left wall of the upper fixing plate (27), the left wall of the upper blocking block (26) is integrally inclined downwards from the right side to the left side, and the bottom end of the feeding pipe (12) moves downwards to support the upper blocking block (26) to move towards the right side.
4. The process for extracting enzymatic soybean phospholipid as defined in claim 2, wherein the discharging valve comprises a lower blocking block (30), a lower fixing plate (31), a lower spring (32) and a lower connecting rod (33), the lower fixing plate (31) is fixed on the outer wall of the discharging pipe (11) through the lower connecting rod (33), the lower blocking block (30) movably penetrates through the right wall of the discharging pipe (11), the lower spring (32) is connected between the right wall of the lower blocking block (30) and the left wall of the lower fixing plate (31), the left wall of the lower blocking block (30) integrally inclines downwards from the left side to the right side, and the top end of the discharging pipe (13) moves upwards to support the lower blocking block (30) to move rightwards.
5. The process for extracting enzymolysis soybean phospholipid as claimed in claim 2, wherein the driving device (20) is arranged on the left wall of the middle cavity, the output end of the driving device (20) is connected with the fixing plate (21), the driving device (20) can drive the fixing plate (21) to move left and right, the fixing plate (21) is provided with two mounting plates (22), the two mounting plates (22) are distributed along the front and back direction, the mounting plates (22) are provided with clamping units, and the clamping units on the two mounting plates (22) can clamp the leftmost discharging pipe (11).
6. The process for extracting enzymatic soybean phospholipid according to claim 5, wherein the clamping unit comprises an air cylinder and clamping plates (23), the air cylinder is fixed on the mounting plate (22), the output end of the air cylinder is connected with the clamping plates (23), and the discharging pipe (11) can be clamped by the two clamping plates (23).
7. The process for extracting soybean lecithin subjected to enzymolysis according to claim 2, wherein the exhaust pipe group comprises a plurality of exhaust pipes (19), and the plurality of exhaust pipes (19) in the exhaust pipe group are distributed around the circumference of the rotating shaft (5).
8. The process for extracting soybean lecithin as claimed in claim 2, wherein the plurality of exhaust ducts (19) in the exhaust duct group are uniformly distributed around the circumference of the rotary shaft (5).
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| CN202210085101.8A CN114478621B (en) | 2022-01-25 | 2022-01-25 | Process for extracting enzymatic soybean phospholipids |
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| CN202210085101.8A CN114478621B (en) | 2022-01-25 | 2022-01-25 | Process for extracting enzymatic soybean phospholipids |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1733775A (en) * | 2005-08-10 | 2006-02-15 | 武汉湖工大膜技术开发有限公司 | Method for phosphatide extraction from soybean vegetable oil |
| US20090255872A1 (en) * | 2006-05-23 | 2009-10-15 | Otv Sa | Aerating device for a water filtering system with immersed membranes, including a floor provided with means for injecting a gas and at least one pressure balancing system |
| CN102827685A (en) * | 2012-09-17 | 2012-12-19 | 东北农业大学 | Method for extracting soybean phospholipids through aqueous enzymatic method |
| KR101712453B1 (en) * | 2016-05-31 | 2017-03-07 | 주식회사 토디팜코리아 | Method for preparing bean curd with nipa fruticans wurmb and bean curd by the method |
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2022
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| CN1733775A (en) * | 2005-08-10 | 2006-02-15 | 武汉湖工大膜技术开发有限公司 | Method for phosphatide extraction from soybean vegetable oil |
| US20090255872A1 (en) * | 2006-05-23 | 2009-10-15 | Otv Sa | Aerating device for a water filtering system with immersed membranes, including a floor provided with means for injecting a gas and at least one pressure balancing system |
| CN102827685A (en) * | 2012-09-17 | 2012-12-19 | 东北农业大学 | Method for extracting soybean phospholipids through aqueous enzymatic method |
| KR101712453B1 (en) * | 2016-05-31 | 2017-03-07 | 주식회사 토디팜코리아 | Method for preparing bean curd with nipa fruticans wurmb and bean curd by the method |
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