CN114467927A - Cut rose flower preservative solution and preparation process thereof - Google Patents

Cut rose flower preservative solution and preparation process thereof Download PDF

Info

Publication number
CN114467927A
CN114467927A CN202210280288.7A CN202210280288A CN114467927A CN 114467927 A CN114467927 A CN 114467927A CN 202210280288 A CN202210280288 A CN 202210280288A CN 114467927 A CN114467927 A CN 114467927A
Authority
CN
China
Prior art keywords
solution
cut
fresh
preservative solution
flower
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202210280288.7A
Other languages
Chinese (zh)
Inventor
闫辉
刘兰晶
邓婷婷
王群
钱保全
何鑫宇
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shandong Water Conservancy Vocational College
Original Assignee
Shandong Water Conservancy Vocational College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shandong Water Conservancy Vocational College filed Critical Shandong Water Conservancy Vocational College
Priority to CN202210280288.7A priority Critical patent/CN114467927A/en
Publication of CN114467927A publication Critical patent/CN114467927A/en
Pending legal-status Critical Current

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
    • A01N3/02—Keeping cut flowers fresh chemically

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Plant Pathology (AREA)
  • Toxicology (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a cut rose flower preservative solution, which takes a solution of 100 parts of distilled water (by weight) as a reference, and comprises the following components in percentage by volume: sucrose 2% and Ca2+1% concentration of Salicylic Acid (SA) in the solution was set to 50 mg. L‑1The concentration of benzalkonium chloride (BKC) in the solution was set at 50 mg.L‑1. The invention passes Ca2+Salicylic acid and benzalkonium chloride are combined as A2B2C2，1.0％Ca2+，50mg·L‑1SA，50mg·L‑1BKC, 2% of cane sugar and distilled water are used for preparing cut flower preservative solution, the cut flower vase fresh-keeping time of the Chinese rose subjected to treatment by the preservative solution is greatly prolonged, the MDA accumulation content later stage is low, the cell membrane stability is strong, the antioxidant enzyme activity is high, the promotion and the application are convenient, the problem of peculiar smell caused by oxidation and decay of cut flower cuts can be avoided by the pure preservative solution, and the pure preservative solution is matched with carbonic acid crystals to release CO2So as to keep the activity of the cut rose flowers.

Description

Cut rose flower preservative solution and preparation process thereof
Technical Field
The invention relates to the technical field of cut flower preservation, in particular to a Chinese rose cut flower preservative solution and a preparation process thereof.
Background
With the improvement of living standard of people, home decoration is popularized. People can usually put flower decoration space indoors, and the aesthetic feeling is improved. For example, the cut flowers of Chinese rose are inserted into the bottle body, and in order to prolong the freshness of the cut flowers, the cut flowers need to be soaked in a fresh-keeping solution. During the bottle-inserting process, water metabolism has a great influence on flowering senescence. The moisture balance of the cut flower means that the cut flower is kept in a good state among moisture absorption, transportation and transpiration, and mainly depends on the relative sizes of the water absorption capacity and the transpiration capacity. Because the in-vitro cut flower loses the active water absorption capacity, the promotion of the passive water absorption taking the transpiration tension as the power is important for prolonging the service life of the cut flower. The reduction of the water absorption capacity of the Chinese rose cut flower is caused by various factors, and the specific reasons for causing the blockage are as follows: (1) blockage by wound reaction at the base of the stem; (2) the cut surface can secrete milk or other substances to cause blockage; (3) when the cutting is carried out, air enters the guide pipe to form an air plug; (4) clogging by deposition of gummy sugars in the xylem; (5) substantial catheter cavitation within the stem is caused by blockage of the base of the incision by bacteria or large particulate matter. In addition, the blooming time of the cut rose flowers is affected by substance metabolism, changes in antioxidant enzyme activity, changes in endogenous hormones, changes in cell membranes, influence of sugar, and the like.
The existing cut flower fresh-keeping liquid is inconvenient for cut flower absorption, leads to water loss and withering of the cut flower, and reduces fresh activity, thereby shortening the flowering phase and failing to achieve the effect of natural and full petals when the petals bloom.
Therefore, it is necessary to invent a cut rose flower preservative solution and a preparation process thereof to solve the problems.
Disclosure of Invention
The invention aims to provide a cut rose flower preservative solution and a preparation process thereof, and aims to solve the problems that the existing cut flower preservative solution is inconvenient for cut flower absorption, so that cut flowers lose water and wither, the freshness activity is reduced, the flowering phase is shortened, and the natural and full effects cannot be achieved when petals bloom.
In order to achieve the above purpose, the invention provides the following technical scheme: a cut rose flower preservative solution comprises the following components in percentage by volume based on 100 parts of distilled water (by weight): sucrose 2% and Ca2+0.5% -1.5%, and the solution contains Salicylic Acid (SA) and benzalkonium chloride (BKC), and the concentration of the Salicylic Acid (SA) in the solution is set to be 25 mg.L-1-75mg·L-1The benzalkonium chloride (BKC) is in theThe concentration in the solution was set at 25 mg.L-1-100mg·L-1。
Preferably, the solution comprises, in volume percent, based on 100 parts by weight of distilled water: sucrose 2% and Ca 2+1％。
Preferably, the concentration of Salicylic Acid (SA) in the solution is set to 50 mg.L based on 100 parts by weight of a solution of distilled water-1The concentration of benzalkonium chloride (BKC) in the solution was set at 50 mg.L-1。
Preferably, the Ca2+The content is adjusted by adding calcium chloride into the fresh-keeping liquid.
Preferably, the pH value of the preservation solution is adjusted by plant acid, and the pH value of the preservation solution is less than or equal to 4 after the pH value is adjusted.
A preparation process of a cut rose flower preservative solution comprises the following production steps:
the method comprises the following steps: taking 100 parts of distilled water, taking sucrose and calcium chloride according to a volume ratio, mixing the raw materials, placing the mixture in a sealed container, heating and stirring the mixture to dissolve the sucrose and the calcium chloride in the distilled water;
step two: adding Salicylic Acid (SA) and benzalkonium chloride (BKC) into the above solution, and mixing under stirring;
step three: testing the pH value of the fresh-keeping liquid by using a pH monitor, and adjusting the pH value of the fresh-keeping liquid by using plant acid to enable the fresh-keeping liquid to be in a proper acidic state;
step four: filtering the prepared fresh-keeping liquid, removing impurities contained in the fresh-keeping liquid, and further performing disinfection and sterilization treatment by using an ultraviolet lamp;
step five: the treated fresh-keeping liquid is added with carbonic acid crystals in the using process.
Preferably, in the fourth step, a microfiltration water purifier is adopted to filter the fresh-keeping liquid.
Preferably, the adding time interval of the carbonic acid crystals is 1d, and the adding amount of the carbonic acid crystals is one percent of the volume of the preservative solution.
In the technical scheme, the invention provides the following technical effects and advantages:
by Ca2+Salicylic acid and benzalkonium chloride are combined as A2B2C2，1.0％Ca2+，50mg·L-1SA，50mg·L-1BKC, 2% of cane sugar and distilled water are used for preparing cut flower preservative solution, the cut flower vase fresh-keeping time of the Chinese rose subjected to treatment by the preservative solution is greatly prolonged, the MDA accumulation content later stage is low, the cell membrane stability is strong, the antioxidant enzyme activity is high, the promotion and the application are convenient, the problem of peculiar smell caused by oxidation and decay of cut flower cuts can be avoided by the pure preservative solution, and the pure preservative solution is matched with carbonic acid crystals to release CO2The cut rose flowers are convenient to carry out photosynthesis, the cut is promoted to absorb moisture and nutrition, the vigor is kept, and the flowers are naturally full.
Drawings
In order to more clearly illustrate the embodiments of the present application or technical solutions in the prior art, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments described in the present invention, and other drawings can be obtained by those skilled in the art according to the drawings.
FIG. 1 shows Ca of the present invention2+Influence graph to water absorption amount of black magic cut flower during inserting period;
FIG. 2 shows Ca of the present invention2+Influence diagram of water absorption during the cut film vase inserting period;
FIG. 3 is a graph showing the effect of salicylic acid on the amount of water absorbed during the insertion of a magic black vase according to the present invention;
FIG. 4 is a graph showing the effect of salicylic acid according to the present invention on water uptake during cutting of a movie star vase;
FIG. 5 shows Ca of the present invention2+Salicylic acid and benzalkonium chloride on the water absorption capacity of the black magic cutting vase during the cutting process;
FIG. 6 shows Ca of the present invention2+Salicylic acid and benzalkonium chloride on the water absorption capacity of the star cut vase during the vase period;
FIG. 7 is a graph showing the effect of the novel formulation and refreshment of the present invention on the activities of SOD, POD and CAT of petals during the insertion of a black magic cut flower;
FIG. 8 is a graph showing the effect of novel formulations and availability of the present invention on petal SOD, POD and CAT activities during the vase of a photoscut vase.
Detailed Description
In order to make the technical solutions of the present invention better understood, those skilled in the art will now describe the present invention in further detail with reference to the accompanying drawings.
The invention provides a cut rose flower preservative solution which comprises a solution based on 100 parts by weight of distilled water, and the solution comprises the following components in percentage by volume: sucrose 2% and Ca2+0.5% -1.5%, and the solution contains Salicylic Acid (SA) and benzalkonium chloride (BKC), and the concentration of the Salicylic Acid (SA) in the solution is set to be 25 mg.L-1-75mg·L-1The concentration of benzalkonium chloride (BKC) in the solution is set to 25 mg.L-1-100mg·L-1。
Further, in the above technical means, the Ca2+The content is adjusted by adding calcium chloride into the fresh-keeping liquid.
Further, in the above technical scheme, the pH value of the preservation solution is adjusted by plant acid, and the pH value of the preservation solution is less than or equal to 4 after the pH value is adjusted.
A preparation process of a cut rose flower preservative solution comprises the following production steps:
the method comprises the following steps: taking 100 parts of distilled water, taking sucrose and calcium chloride according to a volume ratio, mixing the raw materials, placing the mixture in a sealed container, heating and stirring the mixture to dissolve the sucrose and the calcium chloride in the distilled water;
step two: adding Salicylic Acid (SA) and benzalkonium chloride (BKC) into the above solution, and mixing under stirring;
step three: testing the pH value of the fresh-keeping liquid by using a pH monitor, and adjusting the pH value of the fresh-keeping liquid by using plant acid to enable the fresh-keeping liquid to be in a proper acidic state;
step four: filtering the prepared fresh-keeping liquid by adopting a microfiltration water purifier, removing impurities contained in the fresh-keeping liquid, and further performing disinfection and sterilization treatment by using an ultraviolet lamp;
step five: adding carbonic acid crystals into the treated fresh-keeping liquid in the using process, wherein the adding interval time of the carbonic acid crystals is 1d, and the adding amount of the carbonic acid crystals is one percent of the volume of the fresh-keeping liquid.
As shown in FIGS. 1-8, treatment experiments were divided into 6 groups of 0, 2, 4, 6, 8 and 10d with 2% sucrose compared to water treatment, and 5 flowers were taken each time for the assay, which was repeated 3 times. When the activity of sugar and related enzyme is measured, the petals with damage on the outer layer are peeled off, the physiological indexes of the petals without damage are measured, and the average value is calculated.
For the cut flowers of two varieties of China rose, black magic and shadow star, the experiment is set as 3 treatments, distilled water + 2% sucrose is used as a control, and Ca is used2+The concentration gradient was 0.5% for treatment 1, 1.0% for treatment 2 and 1.5% for treatment 3, the experiment was divided into 6 times of 0, 2, 4, 6, 8 and 10d, 3 times for each index measurement, and 10 cut flowers were taken for each time. Each group of treatments was placed in a 2L plastic water cup with a total volume of preservative solution of 1L and sealed with a preservative film to prevent moisture evaporation. The treatment method specifically comprises the following steps:
Figure BDA0003557577480000041
Figure BDA0003557577480000051
for the cut flowers of two varieties of China rose, namely magic black and ghost star, the test is set as 3 treatments, distilled water and 2% sucrose are used as controls, and the concentration gradient of salicylic acid is set as treatment 1 and is 25 mg.L-1Treatment 2 was 50 mg. L-1Treatment 3 was 75 mg. L-1Each treatment was repeated 3 times with 10 cut flowers each. Each group of treatments was placed in a 2L plastic water cup with a total volume of 1L of preservative solution and sealed with a preservative film to prevent moisture evaporation. Measuring the water balance and freshness every day from the day when the cut flower is inserted into the solution
Figure BDA0003557577480000052
Rate of change of weight. The treatment method specifically comprises the following steps:
the cut flowers of the two varieties of China rose, Heijiu and Shaxing, were treated in the following 7 treatments, 10 flowers each, 3 times each, and the index was determined. The treatment method specifically comprises the following steps:
Figure BDA0003557577480000053
adding Ca into flos Rosae chinensis of two varieties including Heijialus and ghost star2+SA and BKC, the processing method is as follows: factor A is Ca2+(0.5%, 1.0%, 1.5%), and the factor B is salicylic acid (0.5, 1.0, 1.5 mmol. L.)-1) Factor C benzalkonium chloride (25, 50, 75 mg. L)-1) The orthogonal analysis experiment is carried out in three factors and three levels. The cut flowers of both varieties of roses were treated according to the following 10 treatments, each treatment was repeated 3 times, each repetition being 10 flowers. The treatment method specifically comprises the following steps:
Figure BDA0003557577480000054
Figure BDA0003557577480000061
during the process of each experiment contrast item, the diameter of a corolla, the water absorption capacity and the water balance value, the bent stem index, the soluble reducing sugar of petals, the soluble protein of the petals, the permeability of cell membranes of the petals, the respiration rate of plant cells, the total number of microbial colonies in a vase solution, the monosaccharide content in the petals and the activity of related enzyme of petal sugar metabolism are measured, and the method specifically comprises the following steps:
measuring the diameter of the crown of each cut flower in each treatment when the flower is opened by using a ruler every other day from the day of the cut flower treatment, and taking the average value of the diameters;
and measuring the water balance value and the fresh weight change rate every other day from the day of cut flower treatment, weighing the bottle plus the solution, and obtaining the difference between two continuous weighing results, namely the water absorption capacity of the solution in the period of time. Similarly weighing the flower branches, the solution and the bottle weight, wherein the difference of the two times is the water loss amount, and the difference between the water absorption amount and the water loss amount is the water balance value;
the bent stem degree is classified according to the included angle between the flower stalk and the vertical direction, the included angle is less than or equal to 45 degrees, and the bent stem is grade 1; 2-level bent stems with included angles larger than 45 degrees and smaller than 90 degrees; the included angle is more than or equal to 90 degrees, and the bent stem is 3-grade. The bent stem index is the bent stem grade number (the bent stem number/the total number);
measuring the content of soluble sugar by using a 3, 5-dinitrosalicylic acid (DNS) method, weighing 1.0104g of analytically pure glucose, placing the analytically pure glucose into a small beaker, adding a small amount of distilled water for dissolving, transferring the beaker into a 1000ml volumetric flask, using the distilled water for fixing the volume to 1000ml, uniformly mixing, and storing the beaker in a refrigerator at 4 ℃. Weighing 6.3g of DNS and 262mL of 2M NaOH solution, adding the DNS and the 2M NaOH solution into 500mL of hot water containing 185g of potassium sodium tartrate, adding 5g of crystalline phenol and 5g of sodium sulfite, stirring for dissolving, cooling, adding distilled water to reach the constant volume of 1000mL, and storing in a brown bottle;
taking 7 25mL graduated test tubes, and adding 1 mg/mL test tubes-1The glucose standard solution, distilled water and DNS are prepared into reaction solutions with different glucose contents. Shaking each tube, boiling in water bath for 5min, taking out, cooling to room temperature, diluting with distilled water to 25mL again, shaking, comparing with ultraviolet spectrophotometer at wavelength of 540nm to obtain its absorbance value (see table below), and making standard curve: y is 0.5450 x-0.0433;
Figure BDA0003557577480000071
the method for measuring the soluble protein of the petals of the Chinese rose cut flower adopts a Coomassie brilliant blue G-250 staining method, and the content (Mg/G) of the protein in a sample is C × VTV1 × FW × 1000, wherein C: standard curve values (μ g); VT: total volume of extract (mL); FW: fresh weight of sample (g); v1: sample addition (mL) for measurement;
the permeability of the cell membrane of the petals of the Chinese rose cut flower is measured by a conductivity measuring method, and the relative external permeability (%) of the electrolyte is (treatment conductivity-blank conductivity)/(boiling conductivity-blank conductivity). times.100;
the respiration rate of the Chinese rose cut flower is measured by adopting an airflow method, the Chinese rose cut flower is sealed in a plastic box, and a T-H-110 atmospheric sampling instrument is used for measuring;
measuring the total number of bacterial colonies, namely preparing a sample to be detected into 3 different 10-time incremental dilutions, then respectively taking out 1mL of each dilution, placing the diluted sample in a sterilization plate, mixing the diluted sample with a nutrient agar culture medium, culturing the diluted sample for a certain time (generally 48 hours) at a certain temperature, recording the number of bacterial colonies formed in each plate, and calculating the total number of bacterial colonies contained in each mL of original sample according to the dilution times; the basic operations generally include: dilution of sample-pour plate-incubate 48 hours-count report;
determining the content of soluble sugar by adopting an anthrone colorimetric method; detecting the contents of sucrose, fructose and glucose by a Waters2695 high performance liquid chromatograph, weighing about 2g of petals, putting the petals into a mortar to kill enzyme for 30s in a microwave oven, adding 2mL of 90% ethanol for grinding, washing the mortar for 2 times by using 1mL of 90% ethanol, centrifuging for 15min by 10000g, extracting residues for 1 time by using 6mL of 90% ethanol again, combining supernate, fixing the volume to 25mL, taking 1mL of the residues, and filtering the 1mL of the residues through a 0.45-micrometer filter membrane to be detected. Chromatographic conditions are as follows: equipped with a Waters2414 differential detector, AgilentNH2 column (4.6 ID. times.250 mm), acetonitrile and double distilled water (75:25) as mobile phase at a flow rate of 1.3 mL. min-1The column temperature is 37 ℃, the sample injection amount is 10 mu L, the detection is carried out at the wavelength of 210nm, and the used standard samples are analytically pure sucrose, fructose and glucose. Sucrose Degradation Index (SDI) with hexose/sucrose [ (G + F)/(S)]Represents;
enzyme extraction reference and incorporation methods of Keller, Ludlow (1993) and smithnan (2009); assay reference for Acid Invertase (AI) and Neutral Invertase (NI) in combination with the methods of Ranwala (1991) and smian (2009); sucrose Synthase (SS) and Sucrose Phosphate Synthase (SPS) assay reference and combined with the methods of Rufty (1983) and smith (2009);
experiments show that: sucrose, Ca2+Salicylic acid and benzalkonium chloride can influence the cut rose flowers and prolong the freshness of the cut rose flowers;
as can be seen from the vial run-time, Ca was treated at different concentrations2+Has remarkable prolonging effect on black magic of Chinese rose cut flower and photosites, the prolonging time is 1-1.5 days, and the vase of photosites is inserted due to different varieties of cut flowersThe service life of the flower is longer than that of black magic, the Chinese rose cut-flower black magic and the shadow star maximum flower diameter are not greatly changed by different treatments, but the bent stem phenomenon is slightly changed, the bent stem index is reduced by 0.3, and the data are as follows:
Figure BDA0003557577480000081
the cut rose flowers treated by salicylic acid can effectively prolong the bottle-inserting life of the cut rose flowers, the black magic bottle-inserting life can be prolonged by 1d, and the shadow can be prolonged by 2d, wherein the bottle-inserting life of the two cut rose flowers under the treatment condition of 2 is longest, and the black magic and the shadow respectively reach 9.4d and 11.2 d; under the treatment of salicylic acid, the maximum open flower diameter of the magic black and the ghost does not obviously change, but the full-bloom period of the magic black and the ghost is prolonged, and the open time of the cut flowers can be prolonged, so that the ornamental value of the cut flowers is improved. As can be seen from the data, the improvement of the bottle-inserting quality of black magic and shadow star by the treatment 2 is most prominent, and the specific steps are as follows:
Figure BDA0003557577480000091
the BKC treatment can effectively prolong the vase life of the cut flower, and takes the treatment 5 as the optimal treatment, and the BKC treatment also has obvious influence on the maximum flower diameter of the cut flower. The maximum flower diameter of the 'shadow star' CK is only 6.1cm, the maximum flower diameter of 8-HQ treatment can reach 6.4cm, the maximum flower diameter of BKC treatment can reach 6.9cm, and is 0.8cm larger than the CK. The maximum flower diameter of the 'Heijie' CK is only 5.4cm, the maximum flower diameter of 8-HQ treatment can reach 5.6cm, the maximum flower diameter of BKC treatment can reach 7.5cm, and is 2.1cm larger than CK, which shows that BKC can effectively improve the maximum flower diameter of cut flowers, the cut flowers of China rose treated by BKC can reach the maximum flower diameter 2-3 days earlier than other treatments, and the days of full bloom can be prolonged. The two varieties of cut flowers treated by CK reach the maximum flower diameter respectively on 8 th and 5 th days, the cut flowers treated by 8-HQ reach the maximum flower diameter time respectively on about 7 th day and 6 th day, while the cut flowers treated by BKC reach the maximum flower diameter already on about 5 th day and fourth day, the duration of the full-bloom period reaches about 7-8 days, and the difference with other treatments is obvious, and the method specifically comprises the following steps:
Figure BDA0003557577480000101
the different treatments have obvious influence on the vase service life of the cut rose black magic, the vase service life of the cut rose treated by CK is the shortest and is only 9.5d, and the vase service life of the cut rose treated by CK is the longest and is 4.6d longer than that of CK. In general, the treatment by adding the preservative remarkably improves the service life of the Chinese rose cut flowers, and the difference of each treatment compared with CK reaches a remarkable level. In the treatment of rose cut flower ghost, the vase life of treatment 5 is also the longest, reaches 13.6d, is prolonged by 4.6d compared with CK treatment, and the vase life difference of each treatment is obvious compared with CK. The maximum open flower diameter of the black magic cut flower is 7.34cm of treatment 5, and the treatments 2, 4, 5 and 9 which have better effects on improving the open flower diameter of the cut flower are treatments, wherein the maximum open flower diameter of the black magic cut flower exceeds 7cm, and the treatments are compared with CK. The maximum diameter of the cut flowers opened by the sunflowers was also significantly affected by each treatment, and the maximum diameter of the cut flowers opened by the sunflowers in treatments 4, 5 and 6 was 7.11, 7.10 and 7.13, while the maximum diameter of the cut flowers opened by the sunflowers in the CK treatment was 6.31, which was increased by 0.8 cm. On the 8 th day of the black magic cut flower treatment, the bent stem index of CK reaches 3.0, the bent stem index of other treatments is less than 3.0, the difference of the treatments is obvious compared with CK, and the effects of treatments 2, 4, 5, 6 and 9 are the best, namely 2.0, 1.9, 2.0 and 2.1 respectively. The bent stem index of the cut movie star flower is also 3.0 on the 8 th day of treatment, the maximum bent stem index is achieved, the most obvious effects in each treatment are treatment 4 and treatment 5, the bent stem index is only 1.7 and 1.8, the difference is obvious compared with CK, and the influence of different treatments on the cut rose flower vase quality is specifically as follows:
Figure BDA0003557577480000111
Figure BDA0003557577480000121
Ca2+the influence of salicylic acid and benzalkonium chloride on the cutting quality of the Chinese rose flower is as follows:
Figure BDA0003557577480000122
through the data recorded by the experiment, the black magic cut flower orthogonal test result is as follows:
Figure BDA0003557577480000123
Figure BDA0003557577480000131
the shadow cut orthogonal test result is as follows:
Figure BDA0003557577480000132
Figure BDA0003557577480000141
it can be seen that the main and secondary sequence of the influence of the test factors on the service life of the black magic cut flower vase is RC(1.31)>RA(1.14)>RB(0.98) the most preferable combination is A2B2C2Since the optimum combination does not appear in the treatment test, a verification test is required again, during which A is carried out2B2C2I.e. the best treatment, the combination is also unified with the single factor test results. The shadow star cut-flower test is the same as the analysis of black magic cut-flower result, and the main and secondary sequence of the influence of the service life of the inserted bottle is RC(1.27)>RA(1.16)>RB(0.93), the most preferred combination is A2B2C2And the test result is also unified with the single-factor test result. Influencing factor C is benzalkonium chloride treatment, whichThe method effectively inhibits the propagation of bacterial and mould in the fresh-keeping liquid and prevents bacterial colonies from corroding stems, so that the method is smooth in conduit, and improves the moisture absorption and transportation and becomes the most influential factor of the three factors; factor A is Ca2+，Ca2+The permeability of cell membranes can be reduced, the exosmosis of protein and sugar is reduced, and the effect of endurance power is provided for the cut flower during the vase period; factor C is salicylic acid, and the effect is not prominent in this test;
the best formula is 1.0 percent of Ca2+，50mg·L-1SA，50mg·L-1The optimum formula is compared with the influence of the fresh fruit to the vase quality of the Chinese rose cut flowers by BKC and 2% sucrose, and the optimum formula is as follows:
Figure BDA0003557577480000142
the cut flowers of the two varieties processed by the new formula have long service life and can be processed with good freshness, but the two varieties have mutual advantages on other indexes. The bottle cut life is taken as the most important quality index of the cut flower and is the most convincing index, the purpose of the fresh-keeping liquid is to prolong the ornamental period of the cut flower and improve the value of the cut flower, thereby also achieving certain economic benefit, and the treatment effect of the new formula on the cut rose flower is better than the profitable and fresh effect on the whole;
Ca2+the data of the salicylic acid and the benzalkonium chloride in the matching processing shows that the primary and secondary sequences of the influence of 3 factors on the 'black magic' and 'shadow star' cut flowers are RC>RA>RBIt is explained that benzalkonium chloride is the most important factor, Ca2+Secondly, salicylic acid has less influence, and the optimal combination is A2B2C2(1.0％Ca2+，50mg·L-1SA，50mg·L-1BKC) was consistent with the results of the single factor treatment and was verified, so the combination was the best combination of the cut rose flower preservative formulation, the ingredient of which was (1.0% Ca)2+，50mg·L-1SA，50mg·L-1BKC, 2% sucrose). In the comparison test of the new formula and the profitable fresh, the cut rose flowers treated by the new formula have longer service life than the profitable freshCompared with the long service life, the later stage of MDA accumulation content is lower, the stability of cell membranes is stronger, the activity of antioxidase is higher, and the result shows that 1.0 percent of Ca2+、50mg·L-1SA、50mg·L-1The BKC and the 2% sucrose can be popularized and applied as a preservative formula.
While certain exemplary embodiments of the present invention have been described above by way of illustration only, it will be apparent to those of ordinary skill in the art that the described embodiments may be modified in various different ways without departing from the spirit and scope of the invention. Accordingly, the drawings and description are illustrative in nature and should not be construed as limiting the scope of the invention.

Claims (8)

1. The cut rose flower preservative solution is characterized by comprising the following components in parts by weight: based on a solution of 100 parts by weight of distilled water, the solution comprises, in volume percent: sucrose 2% and Ca2+0.5% -1.5%, and the solution contains Salicylic Acid (SA) and benzalkonium chloride (BKC), and the concentration of the Salicylic Acid (SA) in the solution is set to be 25 mg.L-1-75mg·L-1The concentration of benzalkonium chloride (BKC) in the solution is set to 25 mg.L-1-100mg·L-1。
2. The cut rose flower preservative solution according to claim 1, wherein the preservative solution comprises: based on a solution of 100 parts by weight of distilled water, the solution comprises, in volume percent: sucrose 2% and Ca2+1％。
3. The cut rose flower preservative solution according to claim 1, wherein the preservative solution comprises: the concentration of Salicylic Acid (SA) in the solution was set to 50 mg.L based on 100 parts by weight of distilled water-1The concentration of benzalkonium chloride (BKC) in the solution was set at 50 mg.L-1。
4. The cut rose flower preservative solution according to claim 1, wherein the preservative solution comprises: the Ca2+The content is determined by adding chlorine into the fresh-keeping liquidCalcium transforming and regulating.
5. The cut rose flower preservative solution according to claim 1, wherein the preservative solution comprises: the pH value of the preservation solution is adjusted by plant acid, and the pH value of the preservation solution is less than or equal to 4 after the pH value is adjusted.
6. The process for preparing the fresh-keeping solution for the cut rose flowers according to any one of claims 1 to 5, wherein the fresh-keeping solution comprises the following components: the production steps are as follows:
the method comprises the following steps: taking 100 parts of distilled water, taking sucrose and calcium chloride according to a volume ratio, mixing the raw materials, placing the mixture in a sealed container, heating and stirring the mixture to dissolve the sucrose and the calcium chloride in the distilled water;
step two: adding Salicylic Acid (SA) and benzalkonium chloride (BKC) into the above solution, and mixing under stirring;
step three: testing the pH value of the fresh-keeping liquid by using a pH monitor, and adjusting the pH value of the fresh-keeping liquid by using plant acid to enable the fresh-keeping liquid to be in a proper acidic state;
step four: filtering the prepared fresh-keeping liquid, removing impurities contained in the fresh-keeping liquid, and further performing disinfection and sterilization treatment by using an ultraviolet lamp;
step five: the treated fresh-keeping liquid is added with carbonic acid crystals in the using process.
7. The preparation process of the cut rose flower preservative solution according to any one of claims 6, wherein the preparation process comprises the following steps: and in the fourth step, a microfiltration water purifier is adopted to filter the fresh-keeping liquid.
8. The preparation process of the cut rose flower preservative solution according to any one of claims 6, wherein the preparation process comprises the following steps: the adding time interval of the carbonic acid crystals is 1d, and the adding amount of the carbonic acid crystals is one percent of the volume of the fresh-keeping liquid.
CN202210280288.7A 2022-03-22 2022-03-22 Cut rose flower preservative solution and preparation process thereof Pending CN114467927A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202210280288.7A CN114467927A (en) 2022-03-22 2022-03-22 Cut rose flower preservative solution and preparation process thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202210280288.7A CN114467927A (en) 2022-03-22 2022-03-22 Cut rose flower preservative solution and preparation process thereof

Publications (1)

Publication Number Publication Date
CN114467927A true CN114467927A (en) 2022-05-13

Family

ID=81488712

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202210280288.7A Pending CN114467927A (en) 2022-03-22 2022-03-22 Cut rose flower preservative solution and preparation process thereof

Country Status (1)

Country Link
CN (1) CN114467927A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115119834A (en) * 2022-08-02 2022-09-30 徐州生物工程职业技术学院 Fresh cut flower preservative for peony and preparation method thereof
CN116098145A (en) * 2023-04-11 2023-05-12 云南建投物流有限公司 Method for prolonging storage period of cut rose flowers

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105994257A (en) * 2016-06-16 2016-10-12 广德县建村花木种植家庭农场 Long-acting cut flower preservation method
CN106359370A (en) * 2016-08-30 2017-02-01 惠州学院 Cut flower preserving tablets and preparation method thereof
CN107996564A (en) * 2017-12-21 2018-05-08 常州大学 A kind of fresh cut-flower antistaling agent formula

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105994257A (en) * 2016-06-16 2016-10-12 广德县建村花木种植家庭农场 Long-acting cut flower preservation method
CN106359370A (en) * 2016-08-30 2017-02-01 惠州学院 Cut flower preserving tablets and preparation method thereof
CN107996564A (en) * 2017-12-21 2018-05-08 常州大学 A kind of fresh cut-flower antistaling agent formula

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
LONG LI 等: "Benzalkonium chloride treatments improve water relations of cut roses", 《ACTA ECOLOGICA SINICA》 *
李龙: "月季切花‘黑魔术’与‘影星’品种保鲜技术及其效应的研究", 《山东农业大学硕士学位论文》 *
赵滢 等: "CaCl2对月季切花保鲜效果的研究", 《杨凌职业技术学院学报》 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115119834A (en) * 2022-08-02 2022-09-30 徐州生物工程职业技术学院 Fresh cut flower preservative for peony and preparation method thereof
CN116098145A (en) * 2023-04-11 2023-05-12 云南建投物流有限公司 Method for prolonging storage period of cut rose flowers
CN116098145B (en) * 2023-04-11 2023-06-20 云南建投物流有限公司 Method for prolonging storage period of cut rose flowers

Similar Documents

Publication Publication Date Title
Torre et al. Water loss and postharvest characteristics of cut roses grown at high or moderate relative air humidity
CN114467927A (en) Cut rose flower preservative solution and preparation process thereof
YE et al. Effects of active modified atmosphere packaging on postharvest quality of shiitake mushrooms (Lentinula edodes) stored at cold storage
Guehl et al. Planting stress, water status and non-structural carbohydrate concentrations in Corsican pine seedlings
Jiang et al. Biochemical and physiological changes involved in browning of litchi fruit caused by water loss
CN104222265B (en) A kind of complex antistaling agent and the application in preserving fruit and vegetable utilizing thereof
Dayer et al. Carbohydrate reserve status of M albec grapevines after several years of regulated deficit irrigation and crop load regulation
CN106613702B (en) Method for relieving drought stress of kiwi fruit trees
Doi et al. Water relations of cut roses as influenced by vapor pressure deficits and temperatures
CN106258079B (en) A kind of Kiwi berry seedling breeding method
Goszczynska et al. Long-term cool storage of bud-cut carnations
CN115804372A (en) Water lily cut flower fresh-keeping liquid, fresh-keeping method and application
CN114600874B (en) Use method of Chinese rose cut vase solution
KR20200134409A (en) Preparation method of fermented material of sprout ginseng using magnetized water
CN105505778A (en) Ultralow temperature preservation method for pleurotus eryngii original mother strains
CN109122058A (en) A kind of method for storing and refreshing of Pleurotus eryngii
CN109043101A (en) The preparation method of dried mango
CN107916194A (en) A kind of method that elaeagnus conferta fruits fermented wine is prepared with brown sugar and elaeagnus conferta fruits
Sukhvibul et al. Medium and long term storage of Anigozanthos manglesii (D. Don) pollen
CN112120015A (en) Cut flower preservative and application thereof in preservation of cut lily flowers
Kirk et al. Low-pressure storage of Hibiscus cuttings. Effect on stomatal opening and rooting
DARMAN et al. Development of water melon (Citrullus vulgaris L.) red wine
Beruto et al. Plant development of Ranunculus asiaticus L. tuberous roots is affected by different temperature and oxygen conditions during storage period
Saltveit Exposure to alcohol vapours reduces chilling-induced injury of excised cucumber cotyledons, but not of seedlings or excised hypocotyl segments
El-Quesni et al. Effect of some chemical preservative solutions on water relation and vase life of Schefflera arboricola cut foliage

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination