CN109122058A - A kind of method for storing and refreshing of Pleurotus eryngii - Google Patents
A kind of method for storing and refreshing of Pleurotus eryngii Download PDFInfo
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- CN109122058A CN109122058A CN201810828249.XA CN201810828249A CN109122058A CN 109122058 A CN109122058 A CN 109122058A CN 201810828249 A CN201810828249 A CN 201810828249A CN 109122058 A CN109122058 A CN 109122058A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/70—Harvesting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/80—Accessories for use after harvesting, e.g. scrapers
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/04—Freezing; Subsequent thawing; Cooling
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/14—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
- A23B7/144—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of gases, e.g. fumigation; Compositions or apparatus therefor
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Abstract
The invention discloses a kind of method for storing and refreshing of Pleurotus eryngii.This method retains partial medium when being picking Pleurotus eryngii, is then stored with after the fresh-keeping valve bag sealing of PE-LD in 3-5 DEG C.Method of the invention can effectively maintain the hardness and toughness, the generation for reducing lignin of Pleurotus eryngii, maintain the distinctive tender and crisp mouthfeel of Pleurotus eryngii；And reduce anaphase storage mushroom body malonaldehyde (MDA), superoxide anion (O2-) production quantity, catalase (CAT) activity is promoted to increase, the abnormal metabolisms such as cell membrane lipid peroxidating, accumulated active oxygen caused by environment stress are reduced, the ageing process of storage period Pleurotus eryngii has effectively been delayed；The loss amount for reducing soluble protein, reduced sugar, flavonoids simultaneously, maintains the nutritional ingredient of storage period Pleurotus eryngii to the greatest extent.The present invention extends Pleurotus eryngii shelf life significant effect, and low in cost, easy to operate, easy to spread, provides a kind of new method and preservation technology new approaches for the storage fresh-keeping of the edible mushrooms such as Pleurotus eryngii.
Description
Technical field
The invention belongs to edible fungi storage technical field of preservation of fresh.More particularly to a kind of method for storing and refreshing of Pleurotus eryngii.
Background technique
Pleurotus eryngii (Pleurotuseryngii) is that exploitation cultivation successfully integrates edible, medicinal, dietotherapy rare
Edible fungus variety.Research shows that Pleurotus eryngii is full of nutrition, and contain various bioactive substances, such as single note, polyphenol,
Peptide, steroid substance, polysaccharide and dietary fiber etc., these bioactive substances can be used for preventing and mitigating human diseases such as cancer
Disease, diseases associated with inflammation, hyperlipidemia, diabetes etc..The polysaccharide extracted from Pleurotus eryngii has been proved to have various biologies
The effects of activity such as strengthen immunity, anticancer, reducing blood lipid, anti-oxidant and protection liver.With the rapid development of the factorial production,
The yield of Pleurotus eryngii is significantly promoted, and is a kind of Rare edible fungus of great development prospect.In recent years, as Pleurotus eryngii market needs
The popularization of the increase and factory culture asked, Pleurotus eryngii yield are substantially improved, and the preservation and freshness of Pleurotus eryngii has become its industry can
Key link in sustainable development.Mature Pleurotus eryngii water content is high, organize it is very tender and crisp, under room temperature 3 days outside it after tradition picking
Sight just shows the phenomenon that brown stain, softening, corruption, physiologically then shows as dehydration, the reduction of nutriment ingredient, toughness increase etc.
A series of variation in turn results in the appreciably shorter phenomenon of Pleurotus eryngii shelf life.
Currently, the fresh-keeping skill that edible fungus fresh-keeping technology mostly uses the basic fundamental means of cryopreservation to combine other controllable
Art extends the shelf life of edible mushroom, such as Zhao Chunyan uses a kind of ethylene receptor inhibitor, that is, 1- methyl cyclopropene (1-
Methylcyclopropene, 1-MCP) it handles and adopts rear Pleurotus eryngii to slow down weight-loss ratio, brown stain and the softening degree of Pleurotus eryngii；
Using the method for single hybridization, the breeding long shelf life bacterial strain in strain of Pleurotus eryngii carries out commercialization cultivation to Min KeunKima etc.；
Xie Liyuan etc. optimizes the gas ratio of storage using gas methods in controlled atmospheric packing technology artificial adjustment LDPE preservative film.
But early period can occur Pleurotus eryngii hardness and toughness is significant in storage in existing reported Pleurotus eryngii preservation method
The phenomenon that rising, it is characterized in that content of lignin is significantly increased, there is apparent lignifying in tissue, and Pleurotus eryngii is caused to lose its spy
The tender and crisp mouthfeel having makes it lose edible and commercial value.And adopting rear lignifying is by low temperature, mechanical damage or germ infection etc.
Lignin abnormal accumulation caused by a kind of body itself defense reaction caused by external cause, and cell membrane lipid caused by environment stress
The abnormal metabolisms such as peroxidating, accumulated active oxygen are considered as that activation gardening product adopts an important factor for rear lignifying occurs.At present
It focuses mostly on the gardening products such as fruits and vegetables about the research for adopting rear lignifying both at home and abroad, although being seen in cryopreservation on edible mushroom
The variation of quality caused by lignifying is observed, but not yet carries out further investigation to it.
Summary of the invention
The technical problem to be solved by the present invention is to overcome the defects and deficiency of existing Pleurotus eryngii method for storing and refreshing, provide one
Kind delays damage stress to cause a series of method for storing and refreshing of the quality deteriorations occurred after Pleurotus eryngii is adopted, retains Pleurotus eryngii part
Culture medium and with the freshness protection package of the PE-LD of suitable depth sealing pack after in 4 DEG C of cryopreservations, can effectively keep Pleurotus eryngii peculiar
Tender and crisp mouthfeel, delay Pleurotus eryngii ageing process, maintain Pleurotus eryngii nutritional ingredient；And this method is low in cost, it is easy to operate, easily
In popularization, Shelf-life significant effect.
Above-mentioned purpose of the present invention is achieved through the following technical solutions:
A kind of method for storing and refreshing of Pleurotus eryngii is to retain partial medium when picking Pleurotus eryngii, in cryopreservation after sealing.
Preferably, the method for storing and refreshing of the Pleurotus eryngii is to retain partial medium when picking Pleurotus eryngii, uses PE-LD
After fresh-keeping valve bag sealing, in cryopreservation, it can preferably inhibit to adopt the weight-loss ratio of rear Pleurotus eryngii, hardness, conductivity and third
The increase of dialdehyde content reaches more preferably storage effect.
It is highly preferred that the method for storing and refreshing of the Pleurotus eryngii is to retain partial medium when picking Pleurotus eryngii, PE- is used
After the fresh-keeping valve bag sealing of LD, stored in 3-5 DEG C.
More specifically, as a kind of selectable preferred operations scheme, the method for storing and refreshing packet of Pleurotus eryngii of the invention
Include following operating procedure:
(1) it picks: mature Pleurotus eryngii fructification is picked together together with part mycelia, the mycelia picked retains a part culture
Base；
(2) it handles: the Pleurotus eryngii after picking is fitted into the fresh-keeping valve bag of PE-LD, gas in bag is naturally, sealing freshness protection package；
(3) it stores: being stored in 3-5 DEG C of freezer.
The present invention by after retaining Pleurotus eryngii partial medium, being packed with the freshness protection package sealing of the PE-LD of suitable depth in 4
DEG C storage mode, Pleurotus eryngii caused by the external causes such as the mechanical damage of traditional Softening itself can be reduced to the greatest extent and defendd
Lignin abnormal accumulation caused by reaction, keeps the tender and crisp mouthfeel of Pleurotus eryngii, and reduce cell membrane lipid caused by environment stress
The abnormal metabolisms such as peroxidating, accumulated active oxygen；Effectively inhibit to reduce anaphase storage mushroom body malonaldehyde (MDA), superoxide anion
(O2-) production quantity promotes the activity of catalase (CAT) antioxidase to increase, reduces cell membrane caused by environment stress
The abnormal metabolisms such as lipid peroxidation, accumulated active oxygen have effectively delayed the ageing process of storage period Pleurotus eryngii；Apricot is reduced simultaneously
The respiratory intensity and degree of dehydration of abalone mushroom delay mushroom body aging, and effective Restrain browning occurs, and keep mushroom body surface color.
In addition, particularly preferably, to avoid mushroom body by any machinery damage in this method, in Pleurotus eryngii picking process
Wound, artificial wound etc..
Preferably, the Softening of Pleurotus eryngii are as follows: remove Growth of Pleurotus eryngii container (such as growth bacterium bag) simultaneously from the bottom to top
Culture medium is removed to mycelia part, and retains the culture medium of this part mycelia and mycelia.It can utmostly reduce in this way
For Pleurotus eryngii in the damage of traditional picking process, the integrality for keeping it biologically individual effectively delays damage stress to cause
The generation of quality deterioration, the hardness and toughness, reduction lignin that maintain apricot Bao that Pleurotus eryngii occurs after adopting, maintains Pleurotus eryngii spy
The tender and crisp mouthfeel having.
It is highly preferred that needing to select fructification length for 10-15cm, diameter 45- by cleaning, sorting after Pleurotus eryngii picking
55mm, color be pure white, any surface finish, no disease and pests harm, non-parachute-opening, the Pleurotus eryngii without any mechanical damage.
And if Pleurotus eryngii is picked and stored again after needing to be transported to predetermined processing place, by the apricot Bao before picking
Mushroom connects culture vessel (such as culture bacterium bag), and low temperature is transported to predetermined processing place together.
Preferably, the holding conditions of the Pleurotus eryngii preservation and freshness are 4 DEG C.With the most frequently used at present, most effective low temperature storage
Hiding basic mode further decreases the respiratory intensity and degree of dehydration of Pleurotus eryngii in storage, delays mushroom body aging, effectively presses down
Brown stain processed occurs, and keeps mushroom body surface color；The experimental results showed that, 1 DEG C and 4 DEG C storage can preferably keep Pleurotus eryngii herein
The activity level of cat catalase antioxidase effectively inhibits the rising of malonaldehyde (MDA), superoxide anion (O2-), and
The content of the nutriments such as soluble protein, flavonoids can be significantly maintained, but Pleurotus eryngii content of lignin is significantly high when 1 DEG C of storage
It is stored in 4 DEG C, anaphase storage mushroom body toughness is also higher, and the edible value of Pleurotus eryngii is with regard to relative drop.Therefore, it is stored using 4 DEG C
As preferred.
Preferably, freshness protection package used in the Pleurotus eryngii preservation and freshness is the fresh-keeping valve bag of PE-LD with a thickness of 0.04-
0.06 mm, more preferably 0.05 mm.
Preferably, it is 20 × 30 cm that the fresh-keeping valve bag specification of PE-LD, which is the more preferable specification of 15-25 × 25-35 cm()；It is excellent
Selection of land, dress Pleurotus eryngii 1-4 in a bag (more preferable number is 3).
This seminar the study found that compared to 0.03 mm thickness PE freshness protection package sealing packaging and 1.5% chitosan coating,
0.05 mm PE packing processes storage effect becomes apparent, and can more effectively keep the quality of Pleurotus eryngii.PE-LD(high forces down close
Spend polyethylene) it is nontoxic, tasteless, toughness and cold resistance are preferable, while its freshness protection package is small to the permeability of vapor and air, water suction
Property it is low, in addition can simplify the sealing operation of storage using valve bag, reduce human cost；Pass through suitable depth and material
Freshness protection package packaging is conducive to its spontaneous controlled-atmosphere and acts on, can effectively inhibit the respiration of Pleurotus eryngii；It is wrapped up compared to common preservative film
Packaging, it is slightly higher that PE-LD seals packing processes degree of lignification, but more can preferably keep the activity level of CAT antioxidase, has
Effect inhibits the rising of malonaldehyde (MDA), superoxide anion (O2-), and soluble protein, flavonoids etc. can more significantly be maintained to seek
The content for supporting substance, so that the fresh-keeping effect of Pleurotus eryngii be effectively ensured.
The invention has the following advantages:
The storage that damage stress can be delayed to cause a series of quality deteriorations occurred after Pleurotus eryngii is adopted the present invention provides one kind is protected
Fresh method retains Pleurotus eryngii partial medium and stores after being packed with the sealing of the freshness protection package of the PE-LD of suitable depth in 4 DEG C of low temperature
Hiding can effectively keep the distinctive tender and crisp mouthfeel of Pleurotus eryngii, delay Pleurotus eryngii ageing process, maintain Pleurotus eryngii nutritional ingredient.The party
In method, retain the mode of Pleurotus eryngii partial medium, the mechanical damage of traditional conventional Softening can be reduced to the greatest extent
Lignin abnormal accumulation caused by Pleurotus eryngii itself defense reaction caused by equal external causes, keeps the tender and crisp mouthfeel of Pleurotus eryngii, and
Reduce the abnormal metabolisms such as cell membrane lipid peroxidating, accumulated active oxygen caused by environment stress；4 DEG C of cryopreservation can be compared with simultaneously
The activity level of Pleurotus eryngii cat catalase antioxidase, effectively inhibition malonaldehyde (MDA), superoxide anion are kept well
(O2-) rising, the significantly content of maintenance soluble protein, the nutriments such as flavonoids, can more significantly inhibit Pleurotus eryngii toughness,
The rising of content of lignin guarantees the edible value of Pleurotus eryngii；In addition, the PE-LD freshness protection package of suitable depth seals packaging, it is conducive to
The spontaneous controlled-atmosphere of Pleurotus eryngii acts on, and further can effectively inhibit the respiration of Pleurotus eryngii, CAT can be kept more significantly anti-oxidant
The activity level of enzyme, effectively inhibits the rising of malonaldehyde (MDA), superoxide anion (O2-), and can more significantly remain soluble
The content of the nutriments such as albumen, flavonoids, so that the fresh-keeping effect of Pleurotus eryngii be effectively ensured.
Pleurotus eryngii method for storing and refreshing of the invention extends Pleurotus eryngii shelf life significant effect, and low in cost, operation side
Just, easy to spread, a kind of new method is provided for the storage fresh-keeping of Pleurotus eryngii, also provides one for the preservation technology of edible mushroom
Kind new approaches.
Detailed description of the invention
Fig. 1 is embodiment and comparative example Pleurotus eryngii firmness change curve graph.
Fig. 2 is embodiment and comparative example Pleurotus eryngii change in toughness curve graph.
Fig. 3 is embodiment and comparative example Pleurotus eryngii content of lignin change curve.
Fig. 4 is embodiment and comparative example Pleurotus eryngii malonaldehyde (MDA) change curve.
Fig. 5 is embodiment and comparative example Pleurotus eryngii superoxide anion (O2-) change curve.
Fig. 6 is embodiment and comparative example Pleurotus eryngii catalase (CAT) change curve.
Fig. 7 is embodiment and comparative example Pleurotus eryngii soluble protein change curve.
Fig. 8 is embodiment and comparative example Pleurotus eryngii reduced sugar change curve.
Fig. 9 is embodiment and comparative example Pleurotus eryngii flavonoids change curve.
Specific embodiment
The present invention is further illustrated below in conjunction with Figure of description and specific embodiment, but embodiment is not to the present invention
It limits in any form.Unless stated otherwise, the present invention uses material, method and apparatus is the art routine materials
Material, method and apparatus.
Unless stated otherwise, following embodiment material therefor and equipment are commercially available.
Following embodiment material therefor of the present invention is as follows:
(1) experimental material: Pleurotus eryngii is purchased from Guangzhou blue sky agricultural Co., Ltd；Freshness protection package: 20 × 30cm of specification, thickness 0.05mm
The fresh-keeping valve bag of PE-LD.
(2) key instrument and equipment: electronic balance DT500；Uv analyzer；Digital display thermostat water bath HH-2；It can be seen that point
Light photometer；Universal16R high speed freezing centrifuge；A-XT plus type Texture instrument (SMS company of Britain).
(3) Pleurotus eryngii before picking the transport of Pleurotus eryngii and selection method: is loaded on modeling together with complete culture bacterium bag
In strand foam case, Agricultural University Of South China is transported to the low temperature lorry that can freeze and is handled and is stored again after required location in the school.
The fructification standard for the mature Pleurotus eryngii selected be length be 10~15cm, diameter about 45~55mm, color are pure white, surface light
Clean, no disease and pests harm, non-parachute-opening, the Pleurotus eryngii without any mechanical damage.
The measuring method of Testing index is as follows in following embodiment of the present invention:
(1) hardness, toughness measurement
It by fructification decaptitating, truncates, mushroom handle portion removes case-hardening part, takes middle section fructification, is cut into 2cm × 2cm
× 1cm size.Texture profile (texture profile analysis, TPA) is referring to Zivanovic etc.
(Zivanovicet al., 2000；Mohapatra et al., 2010) method, use TA-XT plus type Texture instrument
P/36R probe in (SMS company of Britain) carries out TPA test, location parameter toughness (Hardness).Puncture test reference
(the Zivanovic such as Zivanovicet al., 2000) and straight with TA-XT plus type Texture instrument (SMS company of Britain) selection P/5(
2 mm of diameter) probe progress puncture test, the parameter of acquisition is hardness (solidity firmness).Each Pleurotus eryngii takes in the middle part of mushroom body
Same orientation and the sample of size detected, 10 are parallel, and results are averaged.
(2) content of lignin measures
Lignin is referring to (Bruceet al., 1989) method be measured.1g preservation material is weighed, 5mL95% ethyl alcohol is added
Grinding is centrifuged 10min through 5000 × g, and sediment is collected sediment and made it dry, dried object is dissolved in 95% ethyl alcohol flushing 3 times
Then plus 0.9mL 2mol/L it in 25% acetyl bromide glacial acetic acid solution (V/V), jumps a queue in 70 DEG C of thermostat water baths and keeps the temperature 30min,
NaOH terminates reaction, then plus 5mL glacial acetic acid and 0.1mL 7.5mol/L azanol hydrochloric acid, and 10mL is settled to glacial acetic acid,
1000 × g is centrifuged 7min, and supernatant is taken to measure absorption value at 280nm, indicates wood with absorption value of every g fresh weight at 280nm
Lignin content.
(3) malonaldehyde (MDA) assay
Using thiobarbituricacidα- (TBA) colorimetric method (Dong Shugang, 2006).Take the trichloroacetic acid of 2 g sample 5.0 mL, 5 %
The grinding of solution (m/v) ice bath, 4 DEG C of 10000 rpm are centrifuged 15 min, take 2.0 mL supernatants that the sulphur of 2.0 mL, 0.67 % is added
For the solution of trichloroacetic acid (m/v) of barbiturates (10% solution of trichloroacetic acid is prepared) 5%, 5 min of boiling water bath is rapid after taking-up
It is cooling with tap water, take supernatant to measure the light absorption value at 532 nm, 600 nm respectively.As a result it is calculated according to the following formula:
C(μm of ol/g)=V1/1.55 × 10 (A532-A600)-1•a•FW
Wherein: V1: extracting solution total amount (mL), a: measurement extracting solution total amount (mL), FW: material fresh weight (g), 1.55 × 10-1: MDA
Micromole's extinction coefficient.
(4) superoxide anion (O2-) measures
Superoxide anion (O2-) production quantity refers to (Chaitanyaet al., 1994) method and improved.
The drafting of nitrite anions standard curve: taking 8 plug test tube, and number is separately added into 1 mL system concentration
NaNO2(0,5,10,15,20 and 25 μm of ol/L), is separately added into the p-aminobenzene sulfonic acid and 1.0 of 1.0 mL, 17.0 mmol/L
The naphthylamines of 7.0 mmol/L of mL, keeps the temperature 20 min at 25 DEG C, uses No. 0 pipe as blank control, measures OD530 value immediately, with
Nitrate concentration and the OD530 value measured function construction each other, are made nitrite anions standard curve.
Sample measurement: Pleurotus eryngii sample 1g is taken, 2.5 mL, 100 mmol/L pH7.8 phosphate buffer solution is added
(2.5mL0.1 mmol/L EDTA and 1% polyvinylpyrrolidone, PVPP) after being ground into homogenate, is centrifuged 20 in 10000 × g
min.0.5 mL supernatant is taken, the distilled water of 0.1 mol/L pH7.8 phosphate buffer solution of 0.25mL, 0.25mL are separately added into
It with the hydroxylamine hydrochloride of 1.0 mL, 1 mmol/L, shakes up, 1 h is kept the temperature at 25 DEG C, 2 mL ether are added, mix well in 10000
× g is centrifuged 5min, draws 1 mL of water phase of lower layer；Then the p-aminobenzene sulfonic acid and 1.0 of 1.0 mL, 17.0 mmol/L is added
The naphthylamines of 7.0 mmol/L of mL, keeps the temperature 20 min at 25 DEG C, operates according to method identical with production standard curve, immediately
Measure OD530 value.The production quantity nmol/min/g of O2- in every g sample is calculated by standard curve.
(5) catalase (CAT) determination of activity
CAT crude enzyme liquid extracts: 2g sample taken, the kaliumphosphate buffer of 4 mL 50mmol/L (containing 1%PVPP, pH7.8) is added,
Ice bath grinding homogenate is centrifuged 20 min in 4 DEG C, 10000 r/min, and supernatant is zyme extract, 4 DEG C of preservations.
CAT enzyme activity is referring to (Kato et al., 1987) method measurement.With H2O2For substrate, at 25 DEG C of measurement, 240
Absorbance change at nm.Reaction system is the kaliumphosphate buffer (the same SOD of pH) of 1.5 mL, 0.05 mol/L, 1.0 mL
The H of distilled water, 0.5 mL, 0.3 %2O2Solution (being eventually adding, to start reaction, and timing immediately) and 0.01/0.005mL
Crude enzyme liquid measures the absorbance change situation of (30 sec of interval) in 3 min at 240 nm, with year-on-year buffer diluent system generation
It is used as blank control for crude enzyme liquid (potassium phosphate 2mL, distilled water 1.33mL), reduces by 1.00 as CAT per minute using A240
Unit of activity (U), last energy value are expressed as U/g.
A=[(△ A240/V survey) × V is total]/FW
Wherein, △ A240: the A240 of reduction per minute, V are surveyed: crude enzyme liquid amount (mL) are taken when measurement, V is total: thick zyme extract volume
(mL), FW: sample fresh weight (g).
(6) soluble protein measures
The measurement of soluble protein can refer to Bradford(1976) and Gao Junfeng (2006) method.
The production of protein standard curve: weighing 10 mg bovine serum albumin(BSA)s, is dissolved in distilled water and is settled to 100 mL, system
Obtain the bovine serum albumin(BSA) standard solution of 100 μ g/mL.Take 6 plug test tube, number, respectively draw 0,0.2,0.4,
0.6, the bovine serum albumin(BSA) standard solution of 0.8,1.0 mL, 100 μ g/mL is in test tube, adds distilled water to 1 mL, then plus
Enter 3mL Coomassie brilliant blue, be stored at room temperature 2 min, use No. 0 pipe as blank control, absorbance is measured at 595 nm, repeats 3
It is secondary.
Sample treatment: weighing 2 g samples in mortar, and 5 mL distilled water are added and are fully ground, 4 DEG C, 10000 rpm centrifugation
15 min obtain crude extract.0.2 mL supernatant is drawn, 0.8 mL distilled water and 3 mL Coomassie brilliant blue reagents are added, is mixed, room
Temperature stands 2 min.It is operated according to method identical with production standard curve, measures light absorption value at 595 nm, be repeated 3 times.According to
Standard curve calculates soluble protein content (mg/g) in every g sample.
(7) content of reducing sugar measures
Using 3,5- dinitrosalicylic acid (DNS) colorimetric method (Yang Xin's beauty, 1998).
The preparation of glucose standard curve: taking 7 plug test tube, number, respectively draw 0,0.2,0.4,0.6,
0.8,1.0, the glucose standards solution of 1 mg/mL of 1.2mL is added to 2 mL with distilled water in test tube, DNS reagent is added
2mL is sufficiently mixed uniformly, 5 min is heated in boiling water bath, is immediately placed in the beaker for fill cold water after taking-up and is cooled to room
Temperature, then 25 mL are settled to distilled water, it mixes.At 540 nm of wavelength, uses No. 0 pipe as blank control, measures absorbance,
Draw standard curve.
Sample treatment: weighing 2 g samples in mortar, and 5 mL distilled water are added and are fully ground, 4 DEG C, 10000 rpm centrifugation
15 min obtain crude extract.1.0 mL supernatants are drawn, 2 mL DNS reagents (stablizing in refrigerator one week), boiling water bath are added
5 min add distilled water to be settled to 25 mL.It is operated according to method identical with production standard curve, uses No. 0 pipe as blank pair
According to light absorption value at 540 nm of measurement calculates reducing sugar content (mg/g) in every g sample according to standard curve.
(8) flavonoids
The measurement of Flavonoid Content uses the method (Lin of Lin and Tang et al.et al., 2007).Flavonoids is expressed as
The equivalent of rutin.Rutin solution be used as make standard curve, concentration gradient 0.012,0024,0.048,0.072,
0.096mg/mL, the ethyl alcohol (v/v) that solvent is 70%.1mL standard solution or extract are by the 5%NaNO with 0.3 mL2(w/v).
The 6min and then 10% Al (NO that 0.1 mL is added3)3(w/v), 6 min are placed again.And then it is added 4 mL's 4%
NaOH solution is then diluted to 10 mL with 70% ethyl alcohol (v/v).After 12min, the measurement reaction mixing under the wavelength of 510nm
The absorbance of object.The amount of total flavonoids is expressed as the rutin milliequivalent of every gram of extract.
Embodiment 1
The mature Growth of Pleurotus eryngii bacterium bag selected is removed into culture medium to mycelia part from the bottom to top, and retains this part mycelia
Culture medium, this picking process avoids mushroom body by any mechanical damage, artificial wound etc., the Pleurotus eryngii handled well is packed into
In the fresh-keeping valve bag of PE-LD, 3 every bag, gas in bag is naturally, sealing freshness protection package is stored in the environment of 4 DEG C.It was store until 21 days
Hiding terminates.
3 repetitions are at least arranged in each processing, and every 3d measures an index, and each index is at least measured 3 times, as a result made even
Mean value.Testing index includes: hardness, toughness, content of lignin, malonaldehyde (MDA), superoxide anion (O2-), catalase
(CAT), soluble protein, reduced sugar, flavonoids.
Reference examples 1
The mature Pleurotus eryngii fructification part selected is picked from stem, is modified processing, stem after adopting as early as possible
It cuts smooth, not allow culture medium to be adhered on mushroom body as far as possible, while rejecting mushroom body sundries.The Pleurotus eryngii handled well is packed into
In the fresh-keeping valve bag of PE-LD, 3 every bag, gas in bag is naturally, sealing freshness protection package is stored in the environment of 4 DEG C.It was store until 21 days
Hiding terminates.
3 repetitions are at least arranged in each processing, and every 3d measures an index, and each index is at least measured 3 times, as a result made even
Mean value.Testing index includes: hardness, toughness, content of lignin, malonaldehyde (MDA), superoxide anion (O2-), catalase
(CAT), soluble protein, reduced sugar, flavonoids.
Reference examples 2
The mature Pleurotus eryngii fructification part selected is picked from stem, is modified processing, stem after adopting as early as possible
It cuts smooth, not allow culture medium to be adhered on mushroom body as far as possible, while rejecting mushroom body sundries.By the Pleurotus eryngii handled well to protect
Fresh film wraps up 3 layers, stores in the environment of 4 DEG C.Until storage in 21 days terminates.
3 repetitions are at least arranged in each processing, and every 3d measures an index, and each index is at least measured 3 times, as a result made even
Mean value.Testing index includes: hardness, toughness, content of lignin, malonaldehyde (MDA), superoxide anion (O2-), catalase
(CAT), soluble protein, reduced sugar, flavonoids.
Reference examples 3
The mature Pleurotus eryngii fructification part selected is picked from stem, is modified processing, stem after adopting as early as possible
It cuts smooth, not allow culture medium to be adhered on mushroom body as far as possible, while rejecting mushroom body sundries.The Pleurotus eryngii handled well is packed into
In the fresh-keeping valve bag of PE-LD, 3 every bag, gas in bag is naturally, sealing freshness protection package is stored in the environment of 1 DEG C.It was store until 21 days
Hiding terminates.
3 repetitions are at least arranged in each processing, and every 3d measures an index, and each index is at least measured 3 times, as a result made even
Mean value.Testing index includes: hardness, toughness, content of lignin, malonaldehyde (MDA), superoxide anion (O2-), catalase
(CAT), soluble protein, reduced sugar, flavonoids.
The index test result of above embodiments and comparative example:
As a result as shown in figs 1-9, the final result of comprehensive each factor, preservation method effect of the invention is best, can effectively maintain
The hardness and toughness of apricot Bao, the generation for reducing lignin, maintain the distinctive tender and crisp mouthfeel of Pleurotus eryngii；And reduce anaphase storage mushroom
Body malonaldehyde (MDA), superoxide anion (O2-) production quantity promote catalase (CAT) activity to increase, reduce environment stress
The abnormal metabolisms such as caused cell membrane lipid peroxidating, accumulated active oxygen, effectively delayed the aging of storage period Pleurotus eryngii into
Journey；The loss amount for reducing soluble protein, reduced sugar, flavonoids simultaneously, maintains storage period Pleurotus eryngii to the greatest extent
Nutritional ingredient.
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the present invention,
It should be equivalent substitute mode, be included within the scope of the present invention.
Claims (10)
1. a kind of method for storing and refreshing of Pleurotus eryngii, which is characterized in that picking Pleurotus eryngii when retain partial medium, after sealing in
Cryopreservation.
2. the method for storing and refreshing of Pleurotus eryngii according to claim 1, which is characterized in that retain part when picking Pleurotus eryngii and train
Base is supported, after the fresh-keeping valve bag sealing of PE-LD, in cryopreservation.
3. the method for storing and refreshing of Pleurotus eryngii according to claim 2, which is characterized in that retain part when picking Pleurotus eryngii and train
Base is supported, after the fresh-keeping valve bag sealing of PE-LD, is stored in 3-5 DEG C.
4. the method for storing and refreshing of Pleurotus eryngii according to claim 3, which comprises the following steps:
(1) it picks: Pleurotus eryngii fructification is picked together together with part mycelia, the mycelia picked retains culture medium；
(2) it handles: the Pleurotus eryngii after picking is fitted into the fresh-keeping valve bag of PE-LD, seal；
(3) it stores: being stored in 3-5 DEG C.
5. the method for storing and refreshing of -4 any Pleurotus eryngiis according to claim 1, it is characterised in that: in Pleurotus eryngii picking process
Mushroom body is avoided to be damaged.
6. the method for storing and refreshing of -4 any Pleurotus eryngiis according to claim 1, it is characterised in that: the Softening of Pleurotus eryngii
Are as follows: Growth of Pleurotus eryngii container is removed from the bottom to top and removes culture medium to mycelia part, and retains this part mycelia and mycelia
Culture medium.
7. the method for storing and refreshing of -4 any Pleurotus eryngiis according to claim 1, it is characterised in that: Pleurotus eryngii picking after need through
Removal of impurities, sorting are crossed, selects fructification length for 10-15cm, diameter 45-55mm, color are pure white, any surface finish, no disease and pests harm, not
Parachute-opening, the Pleurotus eryngii without any mechanical damage.
8. according to the method for storing and refreshing of any Pleurotus eryngii of claim 2-4, it is characterised in that: the PE-LD is fresh-keeping certainly
The thickness 0.04-0.06 mm of envelope.
9. the method for storing and refreshing of Pleurotus eryngii according to claim 6, it is characterised in that: the fresh-keeping valve bag specification of PE-LD is
15-25 × 25-35 cm, dress Pleurotus eryngii 1-4 in a bag.
10. the method for storing and refreshing of -4 any Pleurotus eryngiis according to claim 1, it is characterised in that: the storage is at 4 DEG C
Storage.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110122162A (en) * | 2019-04-29 | 2019-08-16 | 成都市农林科学院 | Agrocybe processing method suitable for different distance transport |
| CN113142298A (en) * | 2021-05-06 | 2021-07-23 | 华南农业大学 | Method for delaying lignification of pleurotus eryngii and application |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1125767A (en) * | 1994-12-31 | 1996-07-03 | 般若 | Fresh-keeping method of artificially cultivated commodity Chinese caterpillar fungus and its product |
| JPH10313679A (en) * | 1997-05-17 | 1998-12-02 | Matsushiyu Tec:Kk | Pot for quickly sterilizing mushroom culture medium without causing nonuniformity |
| CN106720239A (en) * | 2016-11-18 | 2017-05-31 | 四川省农业科学院土壤肥料研究所 | The storage practice of pleurotus eryngii |
| CN107857921A (en) * | 2017-10-11 | 2018-03-30 | 中国药科大学 | A kind of preservation method of mushroom |
-
2018
- 2018-07-25 CN CN201810828249.XA patent/CN109122058A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1125767A (en) * | 1994-12-31 | 1996-07-03 | 般若 | Fresh-keeping method of artificially cultivated commodity Chinese caterpillar fungus and its product |
| JPH10313679A (en) * | 1997-05-17 | 1998-12-02 | Matsushiyu Tec:Kk | Pot for quickly sterilizing mushroom culture medium without causing nonuniformity |
| CN106720239A (en) * | 2016-11-18 | 2017-05-31 | 四川省农业科学院土壤肥料研究所 | The storage practice of pleurotus eryngii |
| CN107857921A (en) * | 2017-10-11 | 2018-03-30 | 中国药科大学 | A kind of preservation method of mushroom |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110122162A (en) * | 2019-04-29 | 2019-08-16 | 成都市农林科学院 | Agrocybe processing method suitable for different distance transport |
| CN110122162B (en) * | 2019-04-29 | 2021-08-03 | 成都市农林科学院 | Agrocybe cylindracea treatment method suitable for different-distance transportation |
| CN113142298A (en) * | 2021-05-06 | 2021-07-23 | 华南农业大学 | Method for delaying lignification of pleurotus eryngii and application |
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